DE69310179T2 - Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden - Google Patents
Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotidenInfo
- Publication number
- DE69310179T2 DE69310179T2 DE69310179T DE69310179T DE69310179T2 DE 69310179 T2 DE69310179 T2 DE 69310179T2 DE 69310179 T DE69310179 T DE 69310179T DE 69310179 T DE69310179 T DE 69310179T DE 69310179 T2 DE69310179 T2 DE 69310179T2
- Authority
- DE
- Germany
- Prior art keywords
- polynucleotide
- primer
- sequences
- sequence
- blocker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92307992A | 1992-07-31 | 1992-07-31 | |
| PCT/US1993/007051 WO1994003637A1 (en) | 1992-07-31 | 1993-07-30 | Method for introducing defined sequences at the 3' end of polynucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69310179D1 DE69310179D1 (de) | 1997-05-28 |
| DE69310179T2 true DE69310179T2 (de) | 1997-07-31 |
Family
ID=25448085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69310179T Expired - Fee Related DE69310179T2 (de) | 1992-07-31 | 1993-07-30 | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US5679512A (de) |
| EP (1) | EP0652973B1 (de) |
| JP (1) | JPH07509365A (de) |
| AT (1) | ATE152180T1 (de) |
| CA (1) | CA2141450A1 (de) |
| DE (1) | DE69310179T2 (de) |
| DK (1) | DK0652973T3 (de) |
| ES (1) | ES2104160T3 (de) |
| WO (1) | WO1994003637A1 (de) |
Families Citing this family (87)
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|---|---|---|---|---|
| KR970706902A (ko) * | 1995-09-12 | 1997-12-01 | 로드릭 리차드 제이 | Dna 증폭 및 검정 방법 및 장치(device and method for dna amplification and assay) |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| US6518017B1 (en) * | 1997-10-02 | 2003-02-11 | Oasis Biosciences Incorporated | Combinatorial antisense library |
| US20030165888A1 (en) * | 2001-07-18 | 2003-09-04 | Brown Bob D. | Oligonucleotide probes and primers comprising universal bases for diagnostic purposes |
| CN1280619A (zh) * | 1997-11-28 | 2001-01-17 | 根瑟特公司 | 沙眼衣原体的基因组序列和多肽,其片段以及其用途,特别是用于诊断、预防和治疗感染 |
| US6289229B1 (en) * | 1998-01-20 | 2001-09-11 | Scimed Life Systems, Inc. | Readable probe array for in vivo use |
| EP1051515A2 (de) * | 1998-01-27 | 2000-11-15 | Cytocell Limited | Modifizierte nuklein-säure sonden und dessen verwendungen |
| AU777499B2 (en) * | 1999-04-08 | 2004-10-21 | Gen-Probe Incorporated | Antisense oligonucleotides comprising universal and/or degenerate bases |
| WO2000061807A1 (en) * | 1999-04-08 | 2000-10-19 | Oasis Biosciences, Inc. | Amplification and sequencing primer pairs and use thereof |
| US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| PT1218542E (pt) | 1999-09-13 | 2004-08-31 | Nugen Technologies Inc | Metodos e composicoes para amplificacao isotermica linear de sequencias polinucleotidicas |
| CN1498274A (zh) | 2000-06-26 | 2004-05-19 | Ŧب������˾ | 基于转录的核酸扩增方法和组合物 |
| US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| US6994963B1 (en) * | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
| WO2002029117A2 (en) | 2000-10-06 | 2002-04-11 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
| EP1366191A2 (de) | 2000-12-11 | 2003-12-03 | Alexion Pharmaceuticals, Inc. | 'nested' haarnadelförmige oligonukleotide zur amplifizierung von nukleinsäure |
| CA2430329A1 (en) | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| US6946251B2 (en) | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
| JP2005508135A (ja) * | 2001-03-09 | 2005-03-31 | ニューゲン テクノロジーズ， インコーポレイテッド | Ｒｎａ配列の増幅のための方法および組成物 |
| WO2003016546A1 (en) * | 2001-08-21 | 2003-02-27 | Flinders Technologies Pty Ltd. | Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genome mixtures |
| US7414111B2 (en) * | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
| JP4439262B2 (ja) * | 2001-09-19 | 2010-03-24 | アレクシオン ファーマシューティカルズ， インコーポレイテッド | 操作されたテンプレートおよび単一プライマー増幅におけるそれらの使用 |
| US7045319B2 (en) * | 2001-10-30 | 2006-05-16 | Ribomed Biotechnologies, Inc. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| US20040054162A1 (en) * | 2001-10-30 | 2004-03-18 | Hanna Michelle M. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| WO2003078645A2 (en) * | 2002-03-11 | 2003-09-25 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3’ single stranded portion and uses of these complexes for recombination |
| WO2004011665A2 (en) * | 2002-05-17 | 2004-02-05 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilization of nucleic acids |
| WO2004048594A2 (en) | 2002-11-21 | 2004-06-10 | Epicentre Technologies | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| WO2004092418A2 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| US20050214796A1 (en) * | 2003-10-29 | 2005-09-29 | Hanna Michelle M | Compositions, methods and detection technologies for reiterative oligonucleotide synthesis |
| WO2005065321A2 (en) | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| CA2598307C (en) | 2005-02-26 | 2014-12-30 | Basf Plant Science Gmbh | Expression cassettes for seed-preferential expression in plants |
| CA2621267A1 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
| DE102006020885A1 (de) * | 2006-05-05 | 2007-11-08 | Qiagen Gmbh | Einführung von Sequenzelementen in Nukleinsäuren |
| US20100022403A1 (en) * | 2006-06-30 | 2010-01-28 | Nurith Kurn | Methods for fragmentation and labeling of nucleic acids |
| EP2173872B1 (de) * | 2007-06-29 | 2014-04-02 | CellScript, Inc. | Copy-dna und sense-rna |
| US20090203531A1 (en) * | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
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| CA2724343A1 (en) | 2008-05-15 | 2009-11-19 | Ribomed Biotechnologies, Inc. | Methods and reagents for detecting cpg methylation with a methyl cpg binding protein (mbp) |
| US8211644B2 (en) * | 2008-07-13 | 2012-07-03 | Ribomed Biotechnologies, Inc. | Molecular beacon-based methods for detection of targets using abscription |
| JP2012520086A (ja) | 2009-03-15 | 2012-09-06 | リボムド バイオテクノロジーズ インク | アブスクリプションに基づく分子検出 |
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| DE19177059T1 (de) | 2010-10-01 | 2021-10-07 | Modernatx, Inc. | N1-methyl-pseudouracile enthältendes ribonucleinsäuren sowie ihre verwendungen |
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| MX2014007233A (es) | 2011-12-16 | 2015-02-04 | Moderna Therapeutics Inc | Composiciones de nucleosidos, nucleotidos y acidos nucleicos modificados. |
| EP3578697B1 (de) | 2012-01-26 | 2024-03-06 | Tecan Genomics, Inc. | Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und hocheffizienten erzeugung einer bibliothek |
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| US10501512B2 (en) | 2012-04-02 | 2019-12-10 | Modernatx, Inc. | Modified polynucleotides |
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| CA3075505A1 (en) | 2017-09-11 | 2019-03-14 | Twist Bioscience Corporation | Gpcr binding proteins and synthesis thereof |
| GB2583590A (en) | 2017-10-20 | 2020-11-04 | Twist Bioscience Corp | Heated nanowells for polynucleotide synthesis |
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| JP7191448B2 (ja) | 2018-01-04 | 2022-12-19 | ツイスト バイオサイエンス コーポレーション | Ｄｎａベースのデジタル情報ストレージ |
| AU2019270243A1 (en) | 2018-05-18 | 2021-01-07 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| KR20210148122A (ko) * | 2019-02-25 | 2021-12-07 | 트위스트 바이오사이언스 코포레이션 | 차세대 시퀀싱을 위한 조성물 및 방법 |
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1993
- 1993-07-30 AT AT93917347T patent/ATE152180T1/de active
- 1993-07-30 ES ES93917347T patent/ES2104160T3/es not_active Expired - Lifetime
- 1993-07-30 EP EP93917347A patent/EP0652973B1/de not_active Expired - Lifetime
- 1993-07-30 CA CA002141450A patent/CA2141450A1/en not_active Abandoned
- 1993-07-30 DE DE69310179T patent/DE69310179T2/de not_active Expired - Fee Related
- 1993-07-30 WO PCT/US1993/007051 patent/WO1994003637A1/en active IP Right Grant
- 1993-07-30 DK DK93917347.2T patent/DK0652973T3/da active
- 1993-07-30 JP JP6505390A patent/JPH07509365A/ja not_active Ceased
- 1993-10-20 US US08/140,349 patent/US5679512A/en not_active Expired - Fee Related
-
1995
- 1995-06-07 US US08/475,236 patent/US5683879A/en not_active Expired - Fee Related
- 1995-06-07 US US08/479,745 patent/US6030774A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ATE152180T1 (de) | 1997-05-15 |
| US5679512A (en) | 1997-10-21 |
| US5683879A (en) | 1997-11-04 |
| EP0652973B1 (de) | 1997-04-23 |
| US6030774A (en) | 2000-02-29 |
| ES2104160T3 (es) | 1997-10-01 |
| WO1994003637A1 (en) | 1994-02-17 |
| DE69310179D1 (de) | 1997-05-28 |
| CA2141450A1 (en) | 1994-02-17 |
| JPH07509365A (ja) | 1995-10-19 |
| EP0652973A1 (de) | 1995-05-17 |
| DK0652973T3 (da) | 1997-09-15 |
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Owner name: DADE BEHRING MARBURG GMBH, 35041 MARBURG, DE |
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| 8339 | Ceased/non-payment of the annual fee |