DE60227361D1 - Computer vermitteltes assembly von polynucleotiden kodierend für ein zielgerichtetes polypeptide - Google Patents

Computer vermitteltes assembly von polynucleotiden kodierend für ein zielgerichtetes polypeptide

Info

Publication number
DE60227361D1
DE60227361D1 DE60227361T DE60227361T DE60227361D1 DE 60227361 D1 DE60227361 D1 DE 60227361D1 DE 60227361 T DE60227361 T DE 60227361T DE 60227361 T DE60227361 T DE 60227361T DE 60227361 D1 DE60227361 D1 DE 60227361D1
Authority
DE
Germany
Prior art keywords
polynucleotides encoding
polypeptide
target
polynucleotide
targeted polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60227361T
Other languages
English (en)
Inventor
Glen A Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Biotech Inc
Original Assignee
Centocor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centocor Inc filed Critical Centocor Inc
Application granted granted Critical
Publication of DE60227361D1 publication Critical patent/DE60227361D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/20—Sequence assembly
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00695—Synthesis control routines, e.g. using computer programs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/007—Simulation or vitual synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
DE60227361T 2001-01-19 2002-01-18 Computer vermitteltes assembly von polynucleotiden kodierend für ein zielgerichtetes polypeptide Expired - Lifetime DE60227361D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26269301P 2001-01-19 2001-01-19
PCT/US2002/001649 WO2002081490A2 (en) 2001-01-19 2002-01-18 Computer-directed assembly of a polynucleotide encoding a target polypeptide

Publications (1)

Publication Number Publication Date
DE60227361D1 true DE60227361D1 (de) 2008-08-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE60227361T Expired - Lifetime DE60227361D1 (de) 2001-01-19 2002-01-18 Computer vermitteltes assembly von polynucleotiden kodierend für ein zielgerichtetes polypeptide

Country Status (13)

Country Link
US (9) US20030138782A1 (de)
EP (1) EP1385950B1 (de)
JP (1) JP2004533228A (de)
KR (1) KR100860291B1 (de)
AT (1) ATE399857T1 (de)
AU (1) AU2008201007A1 (de)
CA (1) CA2433463A1 (de)
DE (1) DE60227361D1 (de)
DK (1) DK1385950T3 (de)
ES (1) ES2309175T3 (de)
MX (1) MXPA03006344A (de)
PT (1) PT1385950E (de)
WO (1) WO2002081490A2 (de)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7932025B2 (en) * 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
JP4093157B2 (ja) * 2003-09-17 2008-06-04 株式会社日立製作所 分散検査装置及びホスト検査装置
EP2441769B1 (de) * 2004-05-04 2014-05-07 Repligen Corporation Design, Synthese und Anordnung von synthetischen Nukleinsäuren
US8825411B2 (en) 2004-05-04 2014-09-02 Dna Twopointo, Inc. Design, synthesis and assembly of synthetic nucleic acids
WO2007070726A2 (en) * 2005-12-16 2007-06-21 Gene Oracle, Inc. A gene synthesis kit
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US7872120B2 (en) * 2007-08-10 2011-01-18 Venkata Chalapathi Rao Koka Methods for synthesizing a collection of partially identical polynucleotides
EP2285974A2 (de) 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Gensynthese durch konvergentes zusammenfügen von oligonukleotid-bauteilsätzen
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8158391B2 (en) 2009-05-06 2012-04-17 Dna Twopointo, Inc. Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain
JP6155418B2 (ja) 2009-09-02 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 多重エマルジョンの合体による、流体を混合するためのシステム
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US20110224086A1 (en) * 2010-03-09 2011-09-15 Jose Pardinas Methods and Algorithms for Selecting Polynucleotides For Synthetic Assembly
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA3024250C (en) 2010-11-01 2022-01-04 Bio-Rad Laboratories, Inc. System for forming emulsions
IL280133B2 (en) 2010-11-12 2023-04-01 Gen9 Inc Methods and devices for the synthesis of nucleic acids
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
CN103534360A (zh) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 借助对信号的组合使用进行的多重数字分析
EP3789498A1 (de) 2011-04-25 2021-03-10 Bio-rad Laboratories, Inc. Verfahren zur nukleinsäureanalyse
ES2737957T3 (es) 2011-08-26 2020-01-17 Gen9 Inc Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
US9678948B2 (en) 2012-06-26 2017-06-13 International Business Machines Corporation Real-time message sentiment awareness
US9104656B2 (en) * 2012-07-03 2015-08-11 International Business Machines Corporation Using lexical analysis and parsing in genome research
US9460083B2 (en) 2012-12-27 2016-10-04 International Business Machines Corporation Interactive dashboard based on real-time sentiment analysis for synchronous communication
US9690775B2 (en) 2012-12-27 2017-06-27 International Business Machines Corporation Real-time sentiment analysis for synchronous communication
US10167366B2 (en) 2013-03-15 2019-01-01 Melior Innovations, Inc. Polysilocarb materials, methods and uses
ES2943498T3 (es) 2013-08-05 2023-06-13 Twist Bioscience Corp Genotecas sintetizadas de novo
WO2016019387A1 (en) * 2014-08-01 2016-02-04 The Regents Of The University Of California One-pot multiplex gene synthesis
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
GB2557529A (en) 2015-09-18 2018-06-20 Twist Bioscience Corp Oligonucleic acid variant libraries and synthesis thereof
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
CN109996876A (zh) 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11202002194UA (en) 2017-09-11 2020-04-29 Twist Bioscience Corp Gpcr binding proteins and synthesis thereof
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
SG11202002427UA (en) 2017-10-13 2020-04-29 Ribbon Biolabs Gmbh A novel method for synthesis of polynucleotides using a diverse library of oligonucleotides
KR20240024357A (ko) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
KR20200106067A (ko) 2018-01-04 2020-09-10 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
WO2020208234A1 (en) 2019-04-10 2020-10-15 Ribbon Biolabs Gmbh A library of polynucleotides
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US20230037041A1 (en) * 2019-12-30 2023-02-02 Yuandian Biolabs Co., Ltd. Apparatus and method for preparing nucleic acid sequences using enzyme
US20230056396A1 (en) * 2020-01-17 2023-02-23 Asklepios Biopharmaceutical, Inc. Systems and methods for synthetic regulatory sequence design or production
GB2619548A (en) * 2022-06-10 2023-12-13 Nunabio Ltd Nucleic acid and gene synthesis

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652639A (en) 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
EP0316018A3 (de) * 1985-03-29 1989-07-26 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
JPH03505966A (ja) * 1988-07-14 1991-12-26 ベイラー カレッジ オブ メディシン 生物高分子の固相組立及び再構築方法
US5198346A (en) * 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
ATE143696T1 (de) * 1989-02-28 1996-10-15 Canon Kk Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5837528A (en) * 1989-06-22 1998-11-17 Hoffmann La Roche, Inc. Bacterial strains which overproduce riboflavin
AU644619B2 (en) * 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
US5747334A (en) * 1990-02-15 1998-05-05 The University Of North Carolina At Chapel Hill Random peptide library
DK0550696T3 (da) * 1990-09-28 2001-03-19 Hoffmann La Roche Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus
EP0562025B1 (de) 1990-12-06 2001-02-07 Affymetrix, Inc. (a Delaware Corporation) Verbindungen und ihre Verwendung in einer binären Synthesestrategie
EP0823486A3 (de) * 1991-06-27 2004-02-11 Genelabs Technologies, Inc. Verfahren zur Hemmung der Bindung von DNA-Bindungsproteinen mit Duplex-DNA
AU2580892A (en) * 1991-09-05 1993-04-05 Isis Pharmaceuticals, Inc. Determination of oligonucleotides for therapeutics, diagnostics and research reagents
AU669489B2 (en) * 1991-09-18 1996-06-13 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
DE69233331T3 (de) * 1991-11-22 2007-08-30 Affymetrix, Inc., Santa Clara Kombinatorische Strategien zur Polymersynthese
US5783384A (en) * 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
WO1993024650A1 (en) * 1992-05-28 1993-12-09 Monash University Therapeutic compositions
US5807683A (en) * 1992-11-19 1998-09-15 Combichem, Inc. Combinatorial libraries and methods for their use
WO1994012632A1 (en) * 1992-11-27 1994-06-09 University College London Improvements in nucleic acid synthesis by pcr
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
EP0705334A1 (de) * 1993-06-14 1996-04-10 Basf Aktiengesellschaft Strenge kontrolle der genexpression in eukaryotischen zellen durch auf tetrazyklin ansprechende promotoren
US5591578A (en) * 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
WO1995019567A1 (en) * 1994-01-13 1995-07-20 The Trustees Of Columbia University In The City Of New York Synthetic receptors, libraries and uses thereof
US5738996A (en) * 1994-06-15 1998-04-14 Pence, Inc. Combinational library composition and method
US5663046A (en) * 1994-06-22 1997-09-02 Pharmacopeia, Inc. Synthesis of combinatorial libraries
FR2732693B1 (fr) * 1995-04-06 1997-05-09 Bio Veto Tests Bvt Temoin de revelation de contaminants et procede d'application a la realisation d'un antibiogramme directement effectue sur prelevement
US5807754A (en) * 1995-05-11 1998-09-15 Arqule, Inc. Combinatorial synthesis and high-throughput screening of a Rev-inhibiting arylidenediamide array
US6110457A (en) * 1995-12-08 2000-08-29 St. Louis University Live attenuated vaccines based on cp45 HPIV-3 strain and method to ensure attenuation in such vaccines
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
AU9393398A (en) * 1997-09-16 1999-04-05 Egea Biosciences, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
US7321828B2 (en) * 1998-04-13 2008-01-22 Isis Pharmaceuticals, Inc. System of components for preparing oligonucleotides
NZ510230A (en) * 1998-08-25 2004-01-30 Scripps Research Inst Predicting protein function by electronic comparison of functional site descriptors
AU3210200A (en) * 1999-01-19 2000-08-01 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1728860B1 (de) * 1999-02-19 2010-01-27 febit holding GmbH Verfahren zur Herstellung von Polymeren
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

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Publication number Publication date
US20030138782A1 (en) 2003-07-24
US7966338B2 (en) 2011-06-21
ATE399857T1 (de) 2008-07-15
ES2309175T3 (es) 2008-12-16
US20050118628A1 (en) 2005-06-02
US20050053997A1 (en) 2005-03-10
KR20030077582A (ko) 2003-10-01
DK1385950T3 (da) 2008-11-03
AU2008201007A1 (en) 2008-05-15
WO2002081490A3 (en) 2003-11-27
US20050191648A1 (en) 2005-09-01
MXPA03006344A (es) 2004-12-03
KR100860291B1 (ko) 2008-09-25
JP2004533228A (ja) 2004-11-04
US20060154283A1 (en) 2006-07-13
EP1385950A2 (de) 2004-02-04
US20050244841A1 (en) 2005-11-03
WO2002081490A8 (en) 2004-05-21
CA2433463A1 (en) 2002-10-17
WO2002081490A2 (en) 2002-10-17
US20070054277A1 (en) 2007-03-08
US20040241650A1 (en) 2004-12-02
EP1385950B1 (de) 2008-07-02
PT1385950E (pt) 2008-08-12
US20050221340A1 (en) 2005-10-06

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