DK0550696T3 - Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus - Google Patents

Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Info

Publication number
DK0550696T3
DK0550696T3 DK91920000T DK91920000T DK0550696T3 DK 0550696 T3 DK0550696 T3 DK 0550696T3 DK 91920000 T DK91920000 T DK 91920000T DK 91920000 T DK91920000 T DK 91920000T DK 0550696 T3 DK0550696 T3 DK 0550696T3
Authority
DK
Denmark
Prior art keywords
pct
nucleic acid
enzyme
thermosipho africanus
purified thermostable
Prior art date
Application number
DK91920000T
Other languages
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Richard D Abramson
I Lawrence Greenfield
Fred L Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0550696T3 publication Critical patent/DK0550696T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
DK91920000T 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus DK0550696T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59049090A 1990-09-28 1990-09-28
PCT/US1991/007076 WO1992006202A1 (en) 1990-09-28 1991-09-26 PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(THERMOSIPHO AFRICANUS)

Publications (1)

Publication Number Publication Date
DK0550696T3 true DK0550696T3 (da) 2001-03-19

Family

ID=24362481

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91920000T DK0550696T3 (da) 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Country Status (10)

Country Link
US (1) US5968799A (da)
EP (1) EP0550696B1 (da)
JP (1) JP2582980B2 (da)
AT (1) ATE197718T1 (da)
AU (1) AU658378B2 (da)
CA (1) CA2092317A1 (da)
DE (1) DE69132477T2 (da)
DK (1) DK0550696T3 (da)
ES (1) ES2152923T3 (da)
WO (1) WO1992006202A1 (da)

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US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
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US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
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US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2005030995A1 (en) * 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
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ES2692894T3 (es) 2011-06-08 2018-12-05 Life Technologies Corporation Diseño y desarrollo de nuevos detergentes para su uso en sistemas de PCR
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
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US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
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Also Published As

Publication number Publication date
WO1992006202A1 (en) 1992-04-16
JP2582980B2 (ja) 1997-02-19
EP0550696B1 (en) 2000-11-22
EP0550696A1 (en) 1993-07-14
CA2092317A1 (en) 1992-03-29
AU8907791A (en) 1992-04-28
DE69132477T2 (de) 2001-06-13
US5968799A (en) 1999-10-19
ES2152923T3 (es) 2001-02-16
ATE197718T1 (de) 2000-12-15
AU658378B2 (en) 1995-04-13
JPH06504196A (ja) 1994-05-19
DE69132477D1 (en) 2000-12-28

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