DE60140317D1 - Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden - Google Patents
Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotidenInfo
- Publication number
- DE60140317D1 DE60140317D1 DE60140317T DE60140317T DE60140317D1 DE 60140317 D1 DE60140317 D1 DE 60140317D1 DE 60140317 T DE60140317 T DE 60140317T DE 60140317 T DE60140317 T DE 60140317T DE 60140317 D1 DE60140317 D1 DE 60140317D1
- Authority
- DE
- Germany
- Prior art keywords
- polynucleotides
- kits
- improving
- hybridization rates
- complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25553500P | 2000-12-14 | 2000-12-14 | |
PCT/US2001/048592 WO2002048404A2 (en) | 2000-12-14 | 2001-12-07 | Method and kit for enhancing the association rates of polynucleotides |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60140317D1 true DE60140317D1 (de) | 2009-12-10 |
Family
ID=22968766
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60140317T Expired - Lifetime DE60140317D1 (de) | 2000-12-14 | 2001-12-07 | Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden |
Country Status (8)
Country | Link |
---|---|
US (2) | US7374945B2 (de) |
EP (1) | EP1377679B1 (de) |
JP (1) | JP2004537263A (de) |
AT (1) | ATE447043T1 (de) |
AU (2) | AU3090102A (de) |
CA (1) | CA2436857A1 (de) |
DE (1) | DE60140317D1 (de) |
WO (1) | WO2002048404A2 (de) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040265823A1 (en) * | 2001-08-24 | 2004-12-30 | Atsushi Maruyama | Method of judging mismatch between single-stranded nucleic acid molecules |
US6919180B2 (en) * | 2002-03-22 | 2005-07-19 | Sigma Genosys, L.P. | Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents |
DE10237284A1 (de) * | 2002-08-14 | 2004-03-04 | Advalytix Ag | Nukleinsäurenachweis |
MXPA05013658A (es) * | 2003-06-11 | 2006-03-02 | Hybridon Inc | Oligonucleotidos inmunomoduladores estabilizados. |
JP5020825B2 (ja) * | 2004-12-08 | 2012-09-05 | ジェン−プローブ・インコーポレーテッド | 複数型のヒトパピローマウイルスに由来する核酸の検出 |
ES2381279T3 (es) * | 2005-05-06 | 2012-05-24 | Gen-Probe Incorporated | Métodos y productos para la captura de ácido nucleico diana |
US7488581B2 (en) * | 2006-03-20 | 2009-02-10 | Kabushiki Kaisha Toshiba | Method for detecting a target nucleic acid sequence |
ES2668555T3 (es) | 2008-05-27 | 2018-05-18 | Dako Denmark A/S | Composiciones y métodos para la detección de aberraciones cromosómicas con tampones de hibridación novedosos |
WO2010097655A1 (en) * | 2009-02-26 | 2010-09-02 | Dako Denmark A/S | Compositions and methods for rna hybridization applications |
EP2761028A1 (de) | 2011-09-30 | 2014-08-06 | Dako Denmark A/S | Hybridisierungszusammensetzungen und -verfahren mit formamid |
CA2850509C (en) | 2011-10-14 | 2023-08-01 | President And Fellows Of Harvard College | Sequencing by structure assembly |
EP3252173A1 (de) | 2011-10-21 | 2017-12-06 | Dako Denmark A/S | Hybridisierungszusammensetzungen und -verfahren |
EP4249605A3 (de) | 2011-12-22 | 2023-11-15 | President And Fellows Of Harvard College | Verfahren zum nachweis von analyten |
US11021737B2 (en) | 2011-12-22 | 2021-06-01 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
KR102282990B1 (ko) | 2013-06-04 | 2021-07-28 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Rna-가이드된 전사 조절 |
GB2559526B (en) | 2015-11-03 | 2021-02-17 | Harvard College | Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix |
CN116200465A (zh) | 2016-04-25 | 2023-06-02 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
GB2570412A (en) * | 2016-08-31 | 2019-07-24 | Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
GB2569252A (en) | 2016-08-31 | 2019-06-12 | Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
CN109789167A (zh) | 2017-04-14 | 2019-05-21 | 哈佛学院董事及会员团体 | 用于产生细胞衍生的微丝网络的方法 |
EP3618748B1 (de) * | 2017-05-05 | 2023-10-25 | Stryker European Operations Limited | Chirurgisches navigationssystem |
CN112770776A (zh) | 2018-07-30 | 2021-05-07 | 瑞德库尔有限责任公司 | 用于样品处理或分析的方法和*** |
WO2020028631A1 (en) * | 2018-08-01 | 2020-02-06 | Gen-Probe Incorporated | Compositions and methods for detecting nucleic acids of epstein-barr virus |
CN109988842B (zh) * | 2019-04-19 | 2022-12-06 | 上海吉玛制药技术有限公司 | 一种寡核苷酸标记探针原位检测pdl1的试剂组与应用 |
JP7394301B2 (ja) * | 2021-12-10 | 2023-12-08 | 日東紡績株式会社 | 二本鎖核酸の融解温度上昇化剤及びその用途 |
KR20240099469A (ko) * | 2021-12-10 | 2024-06-28 | 니토 보세키 가부시기가이샤 | 핵산의 대상 염기 서열 중에 있어서의 변이를 검출하기 위한 방법, 핵산의 증폭을 선택적으로 저해하는 방법, 및 이것들을 실시하기 위한 키트 |
Family Cites Families (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5132207A (en) | 1984-07-05 | 1992-07-21 | Gen-Probe Incorporated | Accelerated nucleic acid reassociation method |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
JPS638396A (ja) * | 1986-06-30 | 1988-01-14 | Wakunaga Pharmaceut Co Ltd | ポリ標識化オリゴヌクレオチド誘導体 |
US4834854A (en) | 1987-08-20 | 1989-05-30 | Fuji Photo Film Co., Ltd. | Method of making an electrophoresis medium |
US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
JPH081095Y2 (ja) | 1987-09-22 | 1996-01-17 | 富士ゼロックス株式会社 | 画像記録装置 |
US5753437A (en) * | 1987-10-13 | 1998-05-19 | The United States Of America As Represented By The Department Of Health & Human Services | Method of diagnosing cancer susceptibility or metastatic potential |
US5589335A (en) | 1989-04-05 | 1996-12-31 | Amoco Corporation | Hybridization promotion reagents |
CA2069948A1 (en) * | 1989-12-01 | 1991-06-02 | Brian Wylie Pontius | Promotion of high specificity molecular assembly |
US5200314A (en) | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
GB9114180D0 (en) | 1991-07-01 | 1991-08-21 | Amersham Int Plc | Enhancement of polynucleotide hybridisation |
US6030954A (en) | 1991-09-05 | 2000-02-29 | University Of Connecticut | Targeted delivery of poly- or oligonucleotides to cells |
WO1995006056A1 (en) * | 1993-08-20 | 1995-03-02 | University Of Medicine & Dentistry Of New Jersey | Bridged polycationic polymer-oligonucleotide conjugates and methods for preparing same |
US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
US5731148A (en) * | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
US6017700A (en) | 1995-08-04 | 2000-01-25 | Bayer Corporation | Cationic oligonucleotides, and related methods of synthesis and use |
US5837466A (en) | 1996-12-16 | 1998-11-17 | Vysis, Inc. | Devices and methods for detecting nucleic acid analytes in samples |
US6153596A (en) | 1996-12-18 | 2000-11-28 | Emory University | Polycationic oligomers |
US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
JP2001514859A (ja) * | 1997-09-04 | 2001-09-18 | バイエル コーポレイション | クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法 |
US6255476B1 (en) * | 1999-02-22 | 2001-07-03 | Pe Corporation (Ny) | Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports |
CA2270106C (en) * | 1999-04-23 | 2006-03-14 | Yousef Haj-Ahmad | Nucleic acid purification and process |
US6281005B1 (en) | 1999-05-14 | 2001-08-28 | Copernicus Therapeutics, Inc. | Automated nucleic acid compaction device |
US6287774B1 (en) | 1999-05-21 | 2001-09-11 | Caliper Technologies Corp. | Assay methods and system |
AU6093300A (en) | 1999-07-14 | 2001-02-05 | Genometrix Genomics Incorporated | Arrays comprising non-covalently associated nucleic acid probes and methods for making and using them |
US6297016B1 (en) * | 1999-10-08 | 2001-10-02 | Applera Corporation | Template-dependent ligation with PNA-DNA chimeric probes |
US6380377B1 (en) * | 2000-07-14 | 2002-04-30 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
US6448407B1 (en) * | 2000-11-01 | 2002-09-10 | Pe Corporation (Ny) | Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis |
US20040265823A1 (en) * | 2001-08-24 | 2004-12-30 | Atsushi Maruyama | Method of judging mismatch between single-stranded nucleic acid molecules |
JP5034027B2 (ja) * | 2005-10-03 | 2012-09-26 | 株式会社Moresco | パーフルオロポリエーテル化合物、およびこれを用いた潤滑剤ならびに磁気ディスク |
-
2001
- 2001-12-07 AU AU3090102A patent/AU3090102A/xx active Pending
- 2001-12-07 WO PCT/US2001/048592 patent/WO2002048404A2/en active Application Filing
- 2001-12-07 EP EP01991158A patent/EP1377679B1/de not_active Expired - Lifetime
- 2001-12-07 JP JP2002550118A patent/JP2004537263A/ja active Pending
- 2001-12-07 DE DE60140317T patent/DE60140317D1/de not_active Expired - Lifetime
- 2001-12-07 US US10/020,596 patent/US7374945B2/en not_active Expired - Fee Related
- 2001-12-07 CA CA002436857A patent/CA2436857A1/en not_active Abandoned
- 2001-12-07 AT AT01991158T patent/ATE447043T1/de not_active IP Right Cessation
- 2001-12-07 AU AU2002230901A patent/AU2002230901B2/en not_active Ceased
-
2003
- 2003-11-12 US US10/712,525 patent/US20040077014A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20020164614A1 (en) | 2002-11-07 |
EP1377679A2 (de) | 2004-01-07 |
JP2004537263A (ja) | 2004-12-16 |
AU2002230901B2 (en) | 2006-09-07 |
US7374945B2 (en) | 2008-05-20 |
AU3090102A (en) | 2002-06-24 |
ATE447043T1 (de) | 2009-11-15 |
WO2002048404A2 (en) | 2002-06-20 |
CA2436857A1 (en) | 2002-06-20 |
WO2002048404A3 (en) | 2003-10-23 |
US20040077014A1 (en) | 2004-04-22 |
EP1377679B1 (de) | 2009-10-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE60140317D1 (de) | Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden | |
ATE419388T1 (de) | Nukleinsäureanreicherung | |
DE50109303D1 (de) | Polysiloxanpolymere, verfahren zu ihrer herstellung und ihre verwendung | |
DE69937108D1 (de) | Verbindungen und Methoden zum Nachweis von Biomolekülen | |
ATE262595T1 (de) | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen | |
WO2002038806A3 (de) | Nachweis von nukleinsäure-polymorphismen | |
ATE304556T1 (de) | Verfahren zur herstellung von amorphen alpha- olefinpolymeren, diese enthaltende zusammensetzungen und verfahren zur herstellung von verbrückten liganden | |
DE60138491D1 (de) | System und verfahren zur dynamischen paketauswahl für bluetooth | |
ATE515575T1 (de) | Verfahren und sonden für den nachweis von krebs | |
DE69427103T2 (de) | Verfahren, Reagenzien und Kits zur Untersuchung von Neisseria gonorrhoeae und Chlamydia trachomatis | |
DE69718054T2 (de) | Verfahren zur aufreinigung von dns | |
DE60332382D1 (de) | Zentrifugalvorrichtung und verfahren zur durchführung von bindungsversuchen | |
DE60139553D1 (de) | Zusammensetzungen, testsätze und methoden zur identifizierung und modulation von typ i diabetes | |
ATE378657T1 (de) | Verfahren zur automatischen sperrung von öffnungen bei einem kraftfahrzeug | |
DE502004007244D1 (de) | Verfahren zum spezifischen schnellnachweis getr nkesch dlicher mikroorganismen | |
WO2001029262A3 (en) | Genotyping reagents, kits and methods of use thereof | |
CA2210769A1 (fr) | Detection de bacteries du genre listeria selon des techniques d'hybridation de sondes nucleiques | |
ATE476526T1 (de) | Verfahren zur geno- und pathotypisierung von pseudomonas aeruginosa | |
DE60329929D1 (de) | Molekulare identifizierung von bakterien des genus streptococcus und verwandte genus | |
DE50110172D1 (de) | Verfahren zur altersbestimmung von individuen | |
DE60123481D1 (de) | Verfahren zur durchführung einer subtraktiven hybridisierung | |
ATE312204T1 (de) | Methoden und zusammensetzungen zur detektion von spezies des mycobacterium avium-komplexes | |
ATE394507T1 (de) | Verfahren zum nachweis von polynukleotiden | |
ATE399214T1 (de) | Verfahren und kit zur identifizierung antibiotika-resistenter bakterienstämme | |
ATE265543T1 (de) | Verfahren zur identifizierung von verbindungen, die den anaphase-fördernden komplex hemmen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |