DE60045059D1 - Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten - Google Patents

Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten

Info

Publication number
DE60045059D1
DE60045059D1 DE60045059T DE60045059T DE60045059D1 DE 60045059 D1 DE60045059 D1 DE 60045059D1 DE 60045059 T DE60045059 T DE 60045059T DE 60045059 T DE60045059 T DE 60045059T DE 60045059 D1 DE60045059 D1 DE 60045059D1
Authority
DE
Germany
Prior art keywords
methods
probes
analyzing
concentration
determining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60045059T
Other languages
English (en)
Inventor
Ryuichiro Kurane
Takahiro Kanagawa
Yoichi Kamagata
Shinya Kurata
Kazutaka Yamada
Toyokazu Yokomaku
Osamu Koyama
Kenta Furusho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Nippon Steel Kankyo Engineering Co Ltd
Original Assignee
National Institute of Advanced Industrial Science and Technology AIST
Nippon Steel Kankyo Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute of Advanced Industrial Science and Technology AIST, Nippon Steel Kankyo Engineering Co Ltd filed Critical National Institute of Advanced Industrial Science and Technology AIST
Application granted granted Critical
Publication of DE60045059D1 publication Critical patent/DE60045059D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
DE60045059T 1999-04-20 2000-04-20 Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten Expired - Lifetime DE60045059D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11160199 1999-04-20

Publications (1)

Publication Number Publication Date
DE60045059D1 true DE60045059D1 (de) 2010-11-18

Family

ID=14565499

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60045059T Expired - Lifetime DE60045059D1 (de) 1999-04-20 2000-04-20 Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten

Country Status (4)

Country Link
US (6) US6699661B1 (de)
EP (1) EP1046717B1 (de)
CA (2) CA2644688C (de)
DE (1) DE60045059D1 (de)

Families Citing this family (132)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673908B1 (en) * 1999-02-22 2004-01-06 Nuvelo, Inc. Tumor necrosis factor receptor 2
CA2644688C (en) 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
WO2001038585A2 (en) * 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
EP1295941B1 (de) * 2000-06-27 2009-12-16 National Institute of Advanced Industrial Science and Technology Neue nukleinsäuresonden und verfahren zum testen von nukleinsäuren unter deren verwendung
ES2267803T3 (es) 2000-08-11 2007-03-16 University Of Utah Research Foundation Sondas de oligonucleotidos marcados individuales.
US7273700B2 (en) 2001-03-27 2007-09-25 National Institute Of Advanced Industrial Science And Technology Nucleic acid probe and novel method of assaying nucleic acid using the same
EP1251183A3 (de) * 2001-04-18 2003-12-10 Exiqon A/S Verbesserte Helfersonden zur Detektion einer Zielsequenz mittels Fangoligonukleotid
US20020197611A1 (en) * 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
DE60230787D1 (de) * 2001-08-31 2009-02-26 Gen Probe Inc Sonden mit affinitätsverschiebung zur quantifizierung von analytenpolynukleotiden
US20050079501A1 (en) * 2002-01-25 2005-04-14 Hisashi Koike Method and apparatus for detecting nucleic acid data
US7081339B2 (en) * 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
US7445893B2 (en) * 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
EP1550717B1 (de) * 2002-05-31 2010-09-08 Nippon Steel Kankyo Engineering Co., Ltd. Neues verfahren zum testen von nukleinsäure unter verwendung von markiertem nukleotid
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー/アクセプター部分が相補鎖上となる標的核酸検出のmet/fretに基づく方法
DE10231684A1 (de) * 2002-07-12 2004-02-12 Micronas Holding Gmbh Verfahren zur Bestimmung der Zahl von Rezeptoren auf einem Träger sowie Biosensor herstellbar nach diesem Verfahren
EP1403382A3 (de) * 2002-08-06 2004-05-06 Roche Diagnostics GmbH Verbesserte Proben für FRET
EP1389638A1 (de) * 2002-08-06 2004-02-18 Roche Diagnostics GmbH Verbesserte Proben für FRET
EP1405920A3 (de) * 2002-10-02 2004-04-14 Roche Diagnostics GmbH Verbesserte FRET Methode
US20070184453A1 (en) * 2002-10-02 2007-08-09 Roche Molecular Systems, Inc Fret process
EP1437416A4 (de) * 2002-10-24 2004-07-14 Ben Gao Verfahren und geräte zur überwachung der nukleinsäurehybridisierung auf einem dna-chip unter verwendung vierdimensionaler parameter
AU2003291144A1 (en) * 2002-11-21 2004-06-18 Primera Biosystems Sampling method and apparatus for amplification reaction analysis
US20050089875A1 (en) * 2002-11-21 2005-04-28 Sention Inc. Quantitative analysis of expression profiling information produced at various stages of an amplification process
US7885889B2 (en) * 2002-12-30 2011-02-08 Fannie Mae System and method for processing data pertaining to financial assets
US7238792B2 (en) * 2003-03-18 2007-07-03 Washington State University Research Foundation Foldable polymers as probes
WO2004104229A2 (en) * 2003-05-16 2004-12-02 Vickery Michael C L Internal control nucleic acid molecule for nucleic acid amplification systems
US20040241667A1 (en) * 2003-05-30 2004-12-02 Chesk William G. Pulse-jet ejection head diagnostic system
WO2005043114A2 (en) * 2003-10-22 2005-05-12 Ron Broide Oligonucleotide probe sets and uses thereof
WO2005049794A2 (en) 2003-11-13 2005-06-02 University Of Georgia Research Foundation, Inc. Methods of characterizing infectious bursal disease virus
EP1698897B1 (de) * 2003-12-19 2010-03-17 Nippon Steel Kankyo Engineering Co., Ltd. Neue gemische zum testen von nukleinsäuren, neues verfahren zum testen von nukleinsäuren unter verwendung davon sowie dafür zu verwendende nukleinsäuresonde
WO2005103249A1 (ja) 2004-04-26 2005-11-03 Wako Pure Chemical Industries, Ltd. 結核菌検出用プライマー及びプローブ、並びにこれを用いたヒト型結核菌の検出方法
US20060110724A1 (en) 2004-11-19 2006-05-25 Burkhardt William Iii Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
WO2006074222A2 (en) * 2005-01-03 2006-07-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
EP2333561A3 (de) * 2005-03-10 2014-06-11 Gen-Probe Incorporated System zum Durchführen von Tests in mehreren Formaten
EP1712639B1 (de) 2005-04-06 2008-08-27 Maurice Stroun Methode zur Krebsdiagnose mittels Nachweis von DNA und RNA im Kreislauf
ES2357902T3 (es) 2005-05-13 2011-05-03 Wako Pure Chemical Industries, Ltd. Cebadores, sondas, procedimientos y usos de los mismos para la detección de mycobacteriium kansasii.
JP2007054048A (ja) * 2005-07-27 2007-03-08 Sony Corp ハイブリダイゼーション検出方法
ATE553220T1 (de) 2006-03-13 2012-04-15 Wako Pure Chem Ind Ltd Verfahren zur erkennung eines mutierten gens
EP1999272B1 (de) * 2006-03-21 2017-11-01 Koninklijke Philips N.V. Mikroelektronische sensorvorrichtung mit sensoranordnung
CN101432426B (zh) 2006-05-02 2012-09-19 和光纯药工业株式会社 胞内分枝杆菌检测用引物和探针、以及使用该引物和探针的胞内分枝杆菌的检测方法
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
CN104531684A (zh) 2006-12-18 2015-04-22 和光纯药工业株式会社 鸟分枝杆菌检测用引物和探针、以及使用它们检测鸟分枝杆菌的方法
JP5026090B2 (ja) * 2007-01-09 2012-09-12 浜松ホトニクス株式会社 免疫クロマト試験片の測定装置
JP4860489B2 (ja) * 2007-01-09 2012-01-25 浜松ホトニクス株式会社 免疫クロマト試験片の測定方法
JP2008180677A (ja) * 2007-01-26 2008-08-07 Konica Minolta Medical & Graphic Inc マイクロチップ検査システム、マイクロチップ検査装置及びプログラム
EP1997907A1 (de) 2007-06-01 2008-12-03 Friesland Brands B.V. Bifidobakterien
US8460874B2 (en) * 2007-07-03 2013-06-11 Genaphora Ltd. Use of RNA/DNA chimeric primers for improved nucleic acid amplification reactions
EP2482076A1 (de) 2007-07-31 2012-08-01 Osaka University Zusammensetzung zum Messen der Bindeaffinität zwischen Nukleinsäure und Testsubstanz und deren Verwendung
WO2009098485A1 (en) * 2008-02-07 2009-08-13 Forensic Sciences Service Ltd Improvements in and relating to analysis
EP2546344A3 (de) 2008-02-08 2013-04-03 Wako Pure Chemical Industries, Ltd. Primer und Sonde zur Detektion von Chlamydophila caviae sowie Verfahren zur Detektion von Chlamydophila caviae damit
ES2650066T3 (es) 2008-05-28 2018-01-16 Wako Pure Chemical Industries, Ltd. Cebador y sonda para la detección de Mycobacterium intracellulare, y método para la detección de Mycobacterium intracellulare empleando el cebador o la sonda
CA2735250A1 (en) * 2008-07-30 2010-02-04 Nippon Steel Kankyo Engineering Co., Ltd. Universal nucleic acid probe set and method for utilization thereof
CA2735246C (en) * 2008-07-30 2015-02-24 Nippon Steel Kankyo Engineering Co., Ltd. Nucleic acid probe set and method of using the same
EP2166470A1 (de) * 2008-09-19 2010-03-24 Corbett Research Pty Ltd Analyse von Schmelzkurven, insbesondere dsDNA- und Proteinschmelzkurven
WO2010091406A2 (en) * 2009-02-09 2010-08-12 Forensic Science Service Limited Improvements in and relating to devices
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
AU2010276236B2 (en) * 2009-07-21 2014-03-20 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
WO2011052754A1 (ja) 2009-10-30 2011-05-05 アークレイ株式会社 Egfr遺伝子多型検出用プローブおよびその用途
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2557156B1 (de) 2010-03-23 2015-02-11 Wako Pure Chemical Industries, Ltd. Primer und sonde zur detektion von chlamydia trachomatis und verfahren zur detektion von chlamydia trachomatis damit
JP5843487B2 (ja) 2010-06-01 2016-01-13 アークレイ株式会社 Vkorc1遺伝子の−1639番目の塩基の多型検出法、ならびにそのための核酸プローブおよびキット
JP5940771B2 (ja) 2010-07-12 2016-06-29 アークレイ株式会社 abl遺伝子多型の検出用プローブおよびその用途
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
DK2623613T3 (en) * 2010-09-21 2016-10-03 Population Genetics Tech Ltd Increasing the reliability of the allele-indications by molecular counting
CN103443625B (zh) * 2010-10-21 2017-12-05 耐克思乐生物科学有限责任公司 成像细胞仪的内部聚焦参考珠
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
EP2697396B1 (de) * 2011-04-12 2019-11-13 Michael Okura Verfahren und vorrichtung für eine hochpräzise genomanalyseplattform mit hybridisierung und verbesserter dissoziation
KR101660322B1 (ko) 2011-04-28 2016-09-29 아크레이 가부시키가이샤 NPM1 유전자의 exon12 변이의 검출용 프로브 및 그 용도
EP2530166B1 (de) * 2011-05-31 2014-07-16 ARKRAY, Inc. Verfahren zum Nachweis mehrerer Nukleotid-Polymorphismen auf einer einzelnen Wellenlänge mit mehreren mit fluoreszierendem Farbstoff modifizierten Oligonukleotiden mit derselben oder einer nahen Nachweiswellenlänge
US8981318B1 (en) 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
SG11201405274WA (en) 2012-02-27 2014-10-30 Cellular Res Inc Compositions and kits for molecular counting
ES2741099T3 (es) 2012-02-28 2020-02-10 Agilent Technologies Inc Método de fijación de una secuencia de recuento para una muestra de ácido nucleico
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
FR2994740B1 (fr) * 2012-08-21 2015-03-27 Horiba Abx Sas Procede de compensation du vieillissement d'un reactif lors de mesures de fluorescence sur des particules, et dispositif d'analyse biologique mettant en œuvre le procede.
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
IL269097B2 (en) 2012-09-04 2024-01-01 Guardant Health Inc Systems and methods for detecting rare mutations and changes in number of copies
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR20230074639A (ko) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和***
CA2934822A1 (en) 2013-12-28 2015-07-02 Guardant Health, Inc. Methods and systems for detecting genetic variants
US9470305B2 (en) * 2014-01-24 2016-10-18 Honda Motor Co., Ltd. Variable speed control systems and methods for walk-behind working machines
JP6495333B2 (ja) 2014-12-19 2019-04-03 栄研化学株式会社 一塩基多型検出用オリゴヌクレオチドプローブ及び一塩基多型検出方法
ES2824700T3 (es) 2015-02-19 2021-05-13 Becton Dickinson Co Análisis unicelular de alto rendimiento que combina información proteómica y genómica
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
US11302416B2 (en) 2015-09-02 2022-04-12 Guardant Health Machine learning for somatic single nucleotide variant detection in cell-free tumor nucleic acid sequencing applications
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
EP3415636B1 (de) 2016-02-09 2020-11-25 Eiken Kagaku Kabushiki Kaisha Verfahren zum nachweis einer zielnukleinsäure und darin verwendete nukleinsäuresonde
CA3019589A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
WO2018057887A1 (en) * 2016-09-23 2018-03-29 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Methods for quantification of polynucleotides
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
KR20210158870A (ko) 2016-09-30 2021-12-31 가던트 헬쓰, 인크. 무세포 핵산의 다중-해상도 분석 방법
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
WO2018089378A1 (en) 2016-11-08 2018-05-17 Cellular Research, Inc. Methods for expression profile classification
SG11201903158RA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for cell label classification
WO2018102346A1 (en) 2016-11-29 2018-06-07 The Regents Of The University Of California Antimicrobial susceptibility testing device and method for use with portable electronic devices
JP7104048B2 (ja) 2017-01-13 2022-07-20 セルラー リサーチ, インコーポレイテッド 流体チャネルの親水性コーティング
WO2018136474A1 (en) * 2017-01-17 2018-07-26 The Regents Of The University Of California Mobile phone based fluorescent multi-well plate reader
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
WO2018162986A2 (en) 2017-03-09 2018-09-13 Elitechgroup B.V. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
EP3668884B1 (de) 2017-08-17 2022-03-09 ELITechGroup Inc. Duplex stabilisierende fluorescenz quencher für nuklein säure sonden.
EP3728636A1 (de) 2017-12-19 2020-10-28 Becton, Dickinson and Company Mit oligonukleotiden assoziierte teilchen
US10871485B2 (en) 2018-04-13 2020-12-22 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
EP4234717A3 (de) 2018-05-03 2023-11-01 Becton, Dickinson and Company Multiomics-probenanalyse mit hohem durchsatz
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
EP3861134A1 (de) 2018-10-01 2021-08-11 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322A3 (de) 2019-01-23 2023-09-20 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
JP2022519045A (ja) 2019-01-31 2022-03-18 ガーダント ヘルス, インコーポレイテッド 無細胞dnaを単離するための組成物および方法
US20220145360A1 (en) * 2019-03-15 2022-05-12 National Institute Of Advanced Industrial Science And Technology Nucleic acid amplification method
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP4004231A1 (de) 2019-07-22 2022-06-01 Becton, Dickinson and Company Test für einzelzell-chromatin-immunpräzipitationssequenzierung
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5030557A (en) * 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
US4945955A (en) * 1988-03-23 1990-08-07 Burr-Brown Corporation Hazardous waste removal devices
US4951990A (en) * 1989-10-03 1990-08-28 The United States Of America As Represented By The Secretary Of The Navy Fifty five gallon drum handling apparatus
US5712125A (en) * 1990-07-24 1998-01-27 Cemv Bioteknik Ab Competitive PCR for quantitation of DNA
US5199472A (en) * 1991-02-06 1993-04-06 Gilco Sales, Inc. Volumetric closed chemical transfer system
GB9119735D0 (en) * 1991-09-16 1991-10-30 Secr Defence Gene probe biosensor method
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5859231A (en) * 1993-09-03 1999-01-12 Duke University Synthesis of oligonucleotides with boranophosphonate linkages
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5766889A (en) 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
EP0803058B1 (de) * 1994-12-30 2008-11-05 Georgetown University Fluoreszenztest zum nachweis der spaltung einer nukleinsäure
US5775542A (en) * 1995-06-07 1998-07-07 Watson Machinery Internationl Self contained drum dumping and hot melt holding tank and method of unloading, melting and dispensing a slug of hot melt material
US5700646A (en) 1995-09-15 1997-12-23 The United States Of America As Represented By The Secretary Of The Army Comprehensive identification scheme for pathogens
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
EP0892808B1 (de) * 1996-04-12 2008-05-14 PHRI Properties, Inc. Sonden, kits und assays
PT912766E (pt) * 1996-06-04 2009-07-16 Univ Utah Res Found Monitorização da hibridização durante a pcr
US5874587A (en) * 1996-06-25 1999-02-23 The Rockefeller University Imidazolium cations, processes for their preparation, and uses therefor
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
AU9220498A (en) * 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6245522B1 (en) * 1999-03-26 2001-06-12 The Regents Of The University Of California Master molecular rheostat switch for cell signaling
CA2644688C (en) 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
US6435226B1 (en) * 1999-12-08 2002-08-20 Mark D. Shaw Method and apparatus for breaching and venting sealed inner containers within a drum
FR2818261B1 (fr) * 2000-12-19 2003-02-21 Dietrich Yves Installation de vidange de futs et procede d'utilisation de ladite installation
US6484761B2 (en) * 2001-04-27 2002-11-26 International Business Machines Corporation Venting device for hazardous material containers
US20030047231A1 (en) * 2001-09-07 2003-03-13 Kerbel Darrell A. Drum enclosure for containing particles
US6685758B1 (en) * 2001-10-23 2004-02-03 Sprague Air Controls, Inc. Material disposal system
US6609870B2 (en) * 2001-10-23 2003-08-26 Memc Electronic Materials, Inc. Granular semiconductor material transport system and process
US6941979B1 (en) * 2003-06-16 2005-09-13 Larry Ray Potts Container drilling apparatus for non-intrusive perforation of pressurized containers

Also Published As

Publication number Publication date
US6492121B2 (en) 2002-12-10
US7429655B2 (en) 2008-09-30
EP1046717B1 (de) 2010-10-06
CA2644688C (en) 2012-11-13
US7094540B2 (en) 2006-08-22
US6495326B2 (en) 2002-12-17
US20030082592A1 (en) 2003-05-01
CA2644688A1 (en) 2000-10-20
US20040063137A1 (en) 2004-04-01
EP1046717A9 (de) 2001-06-27
US20060177856A1 (en) 2006-08-10
US6699661B1 (en) 2004-03-02
US20010000175A1 (en) 2001-04-05
CA2304260C (en) 2009-03-24
EP1046717A2 (de) 2000-10-25
EP1046717A3 (de) 2004-04-21
US7419786B2 (en) 2008-09-02
CA2304260A1 (en) 2000-10-20
US20010000148A1 (en) 2001-04-05

Similar Documents

Publication Publication Date Title
DE60045059D1 (de) Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
DE69808740D1 (de) Verfahren und kit zur quantifizierung und nukleinsäuresequenzierung von nukleinsäureanalyten in einer probe
DE69714630D1 (de) Biosensor und Verfahren zur quantitaven Analyse von biochemischen Substraten
DE69533884D1 (de) Gerät und Verfahren zur Bestimmung der Konzentration der Zielnukleinsäure in PCR
DE59704930D1 (de) Verfahren und Messanordnung zur optischen Bestimmung der totalen Hämoglobinkonzentration
DE69713599D1 (de) Verfahren zur bestimmung von nukleinsäure-sequenzen und diagnostische anwendungen derselben
ATE227847T1 (de) Teststreifen zur visuellen ermittlung der blutglukose-konzentration
DE60035733D1 (de) Einrichtung und Verfahren zur quantitativen Bestimmung der Aenderung in einem Elektrokardiogrammsignal
DE69523693D1 (de) Analysevorrichtung und Verfahren zur Analyse von biologischer Flüssigkeit
DE69942523D1 (de) Verfahren und vorrichtung zur analyse von seismischen daten
DE69823682D1 (de) Verfahren und gerät zur analyse von daten
DE69223848D1 (de) Biosensor und Verfahren zur quantitativen Analyse
DE69222272D1 (de) Biosensor und Verfahren zur quantitativen Analyse
DE69837544D1 (de) Testgerät und methode zur entdeckung von analyten in proben
DE69728017D1 (de) Vorrichtungen und Verfahren zur Erkennung von mehreren Analyten in Proben
DE60208278D1 (de) System und Verfahren zum Testen von Nukleinsäuremolekülen
DE69918401D1 (de) Gerät und Verfahren zur Messung von lebenden Körperinformationen und des Körperfetts und Programmaufzeichnungsträger
DE69936553D1 (de) Verfahren zur Konzentrationsmessung von NOx
ATA59196A (de) Vorrichtung und verfahren zur bestimmung der konzentration von hämoglobinderivaten in einer unverdünnten unhämolysierten vollblutprobe
DE59506053D1 (de) Testset zur analyse von körperflüssigkeiten, verfahren zu seiner herstellung und analyseverfahren
DE69918123D1 (de) Verfahren und reagentiensatz zur erkennung von protein-protein wechselwirkungen
DE50010710D1 (de) Verfahren zur bestimmung von substanzen mittels der evaneszenzfeldmethode
DE69831972D1 (de) Verfahren zur bereitstellung und ermittlung von daten
DE10227599B4 (de) Verfahren und Reagenz zur spezifischen Identifizierung und Quantifizierung von einem oder mehreren Proteinen in einer Probe
ATE359512T1 (de) Biochemisches verfahren und vorrichtung zur bestimmung von eigenschaften von proteinen