SG11201405274WA - Compositions and kits for molecular counting - Google Patents

Compositions and kits for molecular counting

Info

Publication number
SG11201405274WA
SG11201405274WA SG11201405274WA SG11201405274WA SG11201405274WA SG 11201405274W A SG11201405274W A SG 11201405274WA SG 11201405274W A SG11201405274W A SG 11201405274WA SG 11201405274W A SG11201405274W A SG 11201405274WA SG 11201405274W A SG11201405274W A SG 11201405274WA
Authority
SG
Singapore
Prior art keywords
kits
compositions
molecular counting
counting
molecular
Prior art date
Application number
SG11201405274WA
Inventor
Glenn K Fu
Stephen P A Fodor
Julie Wilhelmy
Original Assignee
Cellular Res Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellular Res Inc filed Critical Cellular Res Inc
Publication of SG11201405274WA publication Critical patent/SG11201405274WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
SG11201405274WA 2012-02-27 2013-02-27 Compositions and kits for molecular counting SG11201405274WA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261603921P 2012-02-27 2012-02-27
US201261745385P 2012-12-21 2012-12-21
PCT/US2013/028103 WO2013130674A1 (en) 2012-02-27 2013-02-27 Compositions and kits for molecular counting

Publications (1)

Publication Number Publication Date
SG11201405274WA true SG11201405274WA (en) 2014-10-30

Family

ID=49083250

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201405274WA SG11201405274WA (en) 2012-02-27 2013-02-27 Compositions and kits for molecular counting

Country Status (11)

Country Link
US (3) US10941396B2 (en)
EP (2) EP2820158B1 (en)
JP (2) JP6375230B2 (en)
CN (1) CN104364392B (en)
AU (2) AU2013226081B2 (en)
CA (2) CA2865575C (en)
ES (2) ES2904816T3 (en)
GB (2) GB2513024B (en)
HK (2) HK1194109A1 (en)
SG (1) SG11201405274WA (en)
WO (1) WO2013130674A1 (en)

Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
DK2414547T3 (en) 2009-04-02 2014-06-16 Fluidigm Corp Multiprimer amplification method for bar coding of target nucleic acids
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2013096851A1 (en) 2011-12-22 2013-06-27 President And Fellows Of Harvard College Compositions and methods for analyte detection
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
ES2776673T3 (en) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Methods and uses for molecular tags
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
DE202013012824U1 (en) 2012-09-04 2020-03-10 Guardant Health, Inc. Systems for the detection of rare mutations and a copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
JP2017504307A (en) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド Method and system for digitally counting features on an array
CA2926436A1 (en) 2013-10-07 2015-04-16 Judith Murrah Multimode image and spectral reader
JP6525473B2 (en) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド Compositions and methods for identifying replicate sequencing leads
ES2822125T3 (en) 2013-12-28 2021-04-29 Guardant Health Inc Methods and systems to detect genetic variants
JP6484636B2 (en) 2014-01-16 2019-03-13 イラミーナ インコーポレーテッド Amplicon preparation and sequencing on solid supports
WO2015123608A1 (en) * 2014-02-13 2015-08-20 Cellular Research Methods and compositions for array-based counting
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
CA2940655C (en) 2014-03-18 2020-07-07 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US9897597B2 (en) * 2014-04-23 2018-02-20 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US20160017320A1 (en) * 2014-07-15 2016-01-21 Qiagen Sciences, Llc Semi-random barcodes for nucleic acid analysis
CA2997906A1 (en) * 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
WO2016090148A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of cell secretion profiles
EP3766988B1 (en) 2015-02-19 2024-02-14 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
CN107208158B (en) * 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
WO2016160844A2 (en) * 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) * 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
JP6698708B2 (en) * 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション Methods, systems, compositions, kits, devices, and computer-readable media for molecular tagging
WO2016210191A1 (en) * 2015-06-23 2016-12-29 Tupac Bio, Inc. Computer-implemented method for designing synthetic dna, and terminal, system and computer-readable medium for the same
US10619186B2 (en) 2015-09-11 2020-04-14 Cellular Research, Inc. Methods and compositions for library normalization
WO2017079406A1 (en) 2015-11-03 2017-05-11 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
GB201520341D0 (en) * 2015-11-18 2015-12-30 Randox Lab Ltd And Randox Teoranta Improvements relating to substrates for the attachment of molecules
EP3390658B1 (en) 2015-12-16 2022-08-03 Standard BioTools Inc. High-level multiplex amplification
CN117174167A (en) 2015-12-17 2023-12-05 夸登特健康公司 Method for determining tumor gene copy number by analyzing cell-free DNA
JP6685138B2 (en) 2016-01-27 2020-04-22 シスメックス株式会社 Quality control method for nucleic acid amplification, quality control reagent and reagent kit therefor
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
CN109070130B (en) 2016-04-11 2022-03-22 亚普蒂恩（B V I）公司 Method for marking cellulose products
CN109415761B (en) 2016-04-25 2022-09-20 哈佛学院董事及会员团体 Hybrid chain reaction method for in situ molecular detection
AU2017261189B2 (en) 2016-05-02 2023-02-09 Becton, Dickinson And Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
JP7046007B2 (en) * 2016-05-26 2022-04-01 ベクトン・ディキンソン・アンド・カンパニー How to adjust the molecular label count
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
KR101915701B1 (en) * 2016-06-16 2018-11-07 한국한의학연구원 Method for measuring mutation rate
WO2018045181A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
JP7057348B2 (en) 2016-08-31 2022-04-19 プレジデント アンド フェローズ オブ ハーバード カレッジ A method of combining biomolecule detection with a single assay using fluorescent in situ sequencing
JP7091348B2 (en) * 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10460828B2 (en) * 2016-09-30 2019-10-29 Lifeos Genomics Corporation Method of nucleic acid fragment detection
CN106498040B (en) * 2016-10-12 2018-10-23 浙江大学 A kind of molecular labeling microballon and the unicellular sequencing approach of high throughput based on the molecular labeling microballon
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
EP3440456B1 (en) 2016-10-24 2020-12-23 Ontera Inc. Fractional abundance of polynucleotide sequences in a sample
JP7232180B2 (en) 2016-11-08 2023-03-02 ベクトン・ディキンソン・アンド・カンパニー Methods of expression profile classification
CN117056774A (en) 2016-11-08 2023-11-14 贝克顿迪金森公司 Methods for cell marker classification
KR102489902B1 (en) 2016-11-11 2023-01-19 바이오 래드 래버러토리스 인코오포레이티드 Methods of processing nucleic acid samples
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
EP3538671A1 (en) * 2016-11-11 2019-09-18 Roche Innovation Center Copenhagen A/S Therapeutic oligonucleotides capture and detection
CN110226084A (en) 2016-11-22 2019-09-10 伊索普莱克西斯公司 For the systems, devices and methods of cell capture and its manufacturing method
EP3513346B1 (en) * 2016-11-30 2020-04-15 Microsoft Technology Licensing, LLC Dna random access storage system via ligation
US20180179577A1 (en) * 2016-12-22 2018-06-28 Jiaming HU Lipid-polymer Hybrid Nanoparticle Biochip and Application Thereof
JP7104048B2 (en) 2017-01-13 2022-07-20 セルラー リサーチ， インコーポレイテッド Hydrophilic coating of fluid channels
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US11085071B2 (en) * 2017-02-01 2021-08-10 Spot Biosystems Ltd. Highly stable and specific molecular beacons encapsulated in cationic lipoplex nanoparticles and application thereof
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
US20180291443A1 (en) * 2017-04-11 2018-10-11 Nugen Technologies, Inc. Library Quantitation And Qualification
JP6983906B2 (en) * 2017-04-11 2021-12-17 ニューゲン テクノロジーズ， インコーポレイテッド Quantitative and qualitative library
EP3448396A4 (en) 2017-04-14 2020-01-22 President and Fellows of Harvard College Methods for generation of cell-derived microfilament network
EP3635135A1 (en) 2017-06-05 2020-04-15 Becton, Dickinson and Company Sample indexing for single cells
JP2019024453A (en) * 2017-08-02 2019-02-21 株式会社リコー Rna concentration quantitative method, rna concentration quantitative device, and rna concentration quantitative apparatus
WO2019046307A1 (en) 2017-08-29 2019-03-07 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
CN107447055A (en) * 2017-09-19 2017-12-08 中国动物疫病预防控制中心 Pig parvoviral droplet digital pcr absolute quantitation detection kit
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111492068A (en) 2017-12-19 2020-08-04 贝克顿迪金森公司 Particles associated with oligonucleotides
EP3543351B1 (en) 2018-03-19 2022-08-10 Ricoh Company, Ltd. Nucleic acid sample-contained container, method for producing nucleic acid sample-contained container, and nucleic acid sample
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
ES2945191T3 (en) 2018-05-03 2023-06-29 Becton Dickinson Co High-throughput multi-omics sample analysis
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
JP2022502027A (en) * 2018-09-24 2022-01-11 セカンド ゲノム インコーポレイテッド Compositions, systems, instruments, and methods for validating microbial flora sequence processing and differential abundance analysis via multiple BESPOKE spike-in mixtures.
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (en) 2019-01-23 2023-06-29 Becton Dickinson Co Antibody Associated Oligonucleotides
WO2020178772A1 (en) * 2019-03-04 2020-09-10 King Abdullah University Of Science And Technology Compositions and methods of labeling nucleic acids and sequencing and analysis thereof
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11578322B2 (en) 2019-05-07 2023-02-14 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
KR20220033484A (en) 2019-06-14 2022-03-16 바이오 래드 래버러토리스 인코오포레이티드 Systems and Methods for Automated Single Cell Processing and Analysis
US11578354B2 (en) * 2019-07-16 2023-02-14 Meliolabs Inc. Methods and devices for single-cell based digital high resolution melt
CN114051534A (en) 2019-07-22 2022-02-15 贝克顿迪金森公司 Single cell chromatin immunoprecipitation sequencing assay
WO2021072306A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
JP2023500679A (en) 2019-11-08 2023-01-10 ベクトン・ディキンソン・アンド・カンパニー Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN114096678A (en) * 2020-07-31 2022-02-25 北京寻因生物科技有限公司 Multiple nucleic acid co-labeling support, and preparation method and application thereof
JP2023545555A (en) 2020-10-19 2023-10-30 バイオ－ラド ラボラトリーズ，インコーポレイティド Systems and methods for rapid multiplexed sample processing with application to nucleic acid amplification assays
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN113337585A (en) * 2021-07-23 2021-09-03 苏州英泽生物医药科技有限公司 Small RNA quantitative detection method

Family Cites Families (614)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
US4510244A (en) 1980-04-17 1985-04-09 The Board Of Trustees Of The Leland Stanford Jr. University Cell labeling with antigen-coupled microspheres
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4725536A (en) 1985-09-19 1988-02-16 Genetics Institute, Inc. Reagent polynucleotide complex with multiple target binding regions, and kit and methods
US6150517A (en) 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Method and kits for the amplification and detection of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5656731A (en) 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
JP2650159B2 (en) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー Nucleic acid amplification method
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
AU632760B2 (en) 1988-07-26 1993-01-14 Genelabs Technologies, Inc. Rna and dna amplification techniques
AU4829690A (en) 1988-12-16 1990-07-10 Siska Diagnostics, Inc. Self-sustained, sequence replication system
US5856092A (en) 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5925525A (en) 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
JPH06500471A (en) 1990-08-24 1994-01-20 ザ・ユニバーシティ・オブ・テネシー・リサーチ・コーポレーション DNA amplification fingerprinting
JP3164814B2 (en) 1990-08-27 2001-05-14 科学技術振興事業団 Preparation of homogenized cDNA library
WO1992007095A1 (en) 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
DK0834575T3 (en) 1990-12-06 2002-04-02 Affymetrix Inc A Delaware Corp Identification of nucleic acids in samples
JPH04337446A (en) 1991-05-15 1992-11-25 Hitachi Ltd Method and device for measuring fine grain and constant quantity method
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
WO1993009668A1 (en) 1991-11-22 1993-05-27 Affymax Technology N.V. Combinatorial strategies for polymer synthesis
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5541061A (en) 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
DE69431719T2 (en) 1993-06-25 2003-09-18 Affymetrix Inc N D Ges D Staat HYBRIDIZATION AND SEQUENCING OF NUCLEIC ACIDS
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US6087186A (en) 1993-07-16 2000-07-11 Irori Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
US5500356A (en) 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
EP0758403B1 (en) 1994-05-05 1998-06-24 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US6495518B1 (en) 1994-06-13 2002-12-17 Vanderbilt University Method for importing biologically active molecules into cells
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6406848B1 (en) 1997-05-23 2002-06-18 Lynx Therapeutics, Inc. Planar arrays of microparticle-bound polynucleotides
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6600996B2 (en) 1994-10-21 2003-07-29 Affymetrix, Inc. Computer-aided techniques for analyzing biological sequences
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
ATE340866T1 (en) 1994-10-28 2006-10-15 Gen Probe Inc COMPOSITIONS AND METHODS FOR THE SIMULTANEOUS DETECTION AND QUANTIFICATION OF A MAJORITY OF SPECIFIC NUCLIC ACID SEQUENCES
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
WO1996036436A1 (en) 1995-04-25 1996-11-21 Irori Remotely programmable matrices with memories and uses thereof
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US5733729A (en) 1995-09-14 1998-03-31 Affymetrix, Inc. Computer-aided probability base calling for arrays of nucleic acid probes on chips
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6300063B1 (en) 1995-11-29 2001-10-09 Affymetrix, Inc. Polymorphism detection
US6147205A (en) 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5830712A (en) 1996-02-06 1998-11-03 Allelix Biopharmaceuticals Inc. Selective template deletion method
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
JP2000512744A (en) 1996-05-16 2000-09-26 アフィメトリックス，インコーポレイテッド System and method for detecting label material
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
JPH1021373A (en) 1996-07-05 1998-01-23 Fuji Photo Film Co Ltd Picture analyzing device
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020172953A1 (en) 1996-07-29 2002-11-21 Mirkin Chad A. Movement of biomolecule-coated nanoparticles in an electric field
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6100029A (en) 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US5935793A (en) 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6117631A (en) 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US6368799B1 (en) 1997-06-13 2002-04-09 Affymetrix, Inc. Method to detect gene polymorphisms and monitor allelic expression employing a probe array
US6333179B1 (en) 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
WO1999005323A1 (en) 1997-07-25 1999-02-04 Affymetrix, Inc. Gene expression and evaluation system
US6420108B2 (en) 1998-02-09 2002-07-16 Affymetrix, Inc. Computer-aided display for comparative gene expression
WO1999009218A1 (en) 1997-08-15 1999-02-25 Affymetrix, Inc. Polymorphism detection utilizing clustering analysis
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
WO1999023254A1 (en) 1997-10-31 1999-05-14 Affymetrix, Inc. Expression profiles in adult and fetal organs
US6013449A (en) 1997-11-26 2000-01-11 The United States Of America As Represented By The Department Of Health And Human Services Probe-based analysis of heterozygous mutations using two-color labelling
WO1999028505A1 (en) 1997-12-03 1999-06-10 Curagen Corporation Methods and devices for measuring differential gene expression
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6201639B1 (en) 1998-03-20 2001-03-13 James W. Overbeck Wide field of view and high speed scanning microscopy
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6185030B1 (en) 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6020135A (en) 1998-03-27 2000-02-01 Affymetrix, Inc. P53-regulated genes
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6653077B1 (en) 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US6185561B1 (en) 1998-09-17 2001-02-06 Affymetrix, Inc. Method and apparatus for providing and expression data mining database
US6262216B1 (en) 1998-10-13 2001-07-17 Affymetrix, Inc. Functionalized silicon compounds and methods for their synthesis and use
WO2000024939A1 (en) 1998-10-27 2000-05-04 Affymetrix, Inc. Complexity management and analysis of genomic dna
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6197554B1 (en) 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US20020019005A1 (en) 1999-02-18 2002-02-14 Arcaris, Inc. Process for identification of genes encoding proteins having cell proliferation-promoting activity
US6177248B1 (en) 1999-02-24 2001-01-23 Affymetrix, Inc. Downstream genes of tumor suppressor WT1
US6629040B1 (en) 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
EP1165839A2 (en) 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
CA2304260C (en) 1999-04-20 2009-03-24 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
US6218803B1 (en) 1999-06-04 2001-04-17 Genetic Microsystems, Inc. Position sensing with variable capacitance transducers
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6326148B1 (en) 1999-07-12 2001-12-04 The Regents Of The University Of California Detection of copy number changes in colon cancer
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
JP2001078768A (en) * 1999-09-16 2001-03-27 Otsuka Pharmaceut Co Ltd Assay of gene expression
SE9903988D0 (en) 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of analysis
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US6500620B2 (en) 1999-12-29 2002-12-31 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
WO2001053539A1 (en) 2000-01-24 2001-07-26 Phylos, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
US7022479B2 (en) 2000-01-24 2006-04-04 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
US20030097222A1 (en) 2000-01-25 2003-05-22 Craford David M. Method, system, and computer software for providing a genomic web portal
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
AU2001238068A1 (en) 2000-02-07 2001-08-14 Illumina, Inc. Nucleic acid detection methods using universal priming
US20050214825A1 (en) 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
GB2364054B (en) 2000-03-24 2002-05-29 Smithkline Beecham Corp Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
WO2001073118A2 (en) 2000-03-29 2001-10-04 Lgc (Teddington) Limited Hybridisation beacon and method of rapid sequence detection and discrimination
AU2001253310A1 (en) 2000-04-10 2001-10-23 Matthew Ashby Methods for the survey and genetic analysis of populations
US20030207300A1 (en) 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1362121A2 (en) 2000-08-11 2003-11-19 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE60124363T2 (en) 2000-08-25 2007-09-06 Riken, Wako Method for the production of standardized and / or subtracted cDNA
US6934408B2 (en) 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
ATE380883T1 (en) 2000-10-24 2007-12-15 Univ Leland Stanford Junior DIRECT MULTIPLEX CHARACTERIZATION OF GENOMIC DNA
EP1356109A2 (en) 2000-12-08 2003-10-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
US20030049616A1 (en) 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
WO2002070684A2 (en) 2001-01-11 2002-09-12 Lion Bioscience Ag Gene library for screening methods
US6988040B2 (en) 2001-01-11 2006-01-17 Affymetrix, Inc. System, method, and computer software for genotyping analysis and identification of allelic imbalance
US20020183936A1 (en) 2001-01-24 2002-12-05 Affymetrix, Inc. Method, system, and computer software for providing a genomic web portal
JP4588976B2 (en) 2001-01-25 2010-12-01 ルミネックス モレキュラー ダイアグノスティックス， インク． Polynucleotides, products, and uses thereof as tags and tag complements
US20030120432A1 (en) 2001-01-29 2003-06-26 Affymetrix, Inc. Method, system and computer software for online ordering of custom probe arrays
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
US6804679B2 (en) 2001-03-12 2004-10-12 Affymetrix, Inc. System, method, and user interfaces for managing genomic data
JP2005233974A (en) 2001-03-21 2005-09-02 Olympus Corp Biochemical inspection method
US7027932B2 (en) 2001-03-21 2006-04-11 Olympus Optical Co., Ltd. Biochemical examination method
ATE431853T1 (en) 2001-03-28 2009-06-15 Nanosphere Inc BIO-BAR CODES BASED ON OLIGONUCLEOTIDE-MODIFIED PARTICLES
CA2443894A1 (en) 2001-04-11 2002-10-24 Charlie Xiang Modified random primers for probe labeling
US20030170675A1 (en) 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
AUPR480901A0 (en) 2001-05-04 2001-05-31 Genomics Research Partners Pty Ltd Diagnostic method for assessing a condition of a performance animal
US6849404B2 (en) 2001-05-07 2005-02-01 Bioneer Corporation Polymerase chain reaction of DNA of which base sequence is completely unidentified
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
EP1395805A4 (en) 2001-06-11 2005-03-09 Illumina Inc Multiplexed detection methods
JP4244534B2 (en) 2001-06-12 2009-03-25 横河電機株式会社 Biochip
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US6830931B2 (en) 2001-07-12 2004-12-14 Automated Cell, Inc. Method and apparatus for monitoring of proteins and cells
US20030100995A1 (en) 2001-07-16 2003-05-29 Affymetrix, Inc. Method, system and computer software for variant information via a web portal
US6872529B2 (en) 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
WO2006071776A2 (en) 2004-12-23 2006-07-06 Ge Healthcare Bio-Sciences Corp. Ligation-based rna amplification
CA2463323C (en) 2001-10-09 2010-12-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
WO2003031591A2 (en) 2001-10-10 2003-04-17 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
AU2002359436A1 (en) 2001-11-13 2003-06-23 Rubicon Genomics Inc. Dna amplification and sequencing using dna molecules generated by random fragmentation
US20030165935A1 (en) 2001-11-21 2003-09-04 Vann Charles S. Digital assay
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
US20030120431A1 (en) 2001-12-21 2003-06-26 Affymetrix, Inc. Method and computer software product for genomic alignment and assessment of the transcriptome
US20040002818A1 (en) 2001-12-21 2004-01-01 Affymetrix, Inc. Method, system and computer software for providing microarray probe data
JP2005538929A (en) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ Methods for isolating nucleic acids and proteins from a single sample
CA2474864A1 (en) * 2002-01-29 2003-08-07 Patrik Ernfors Methods and means for amplifying nucleic acid
CA2759764C (en) 2002-02-14 2017-06-13 Veridex, Llc Methods and algorithms for cell enumeration in a low-cost cytometer
CA2422224A1 (en) 2002-03-15 2003-09-15 Affymetrix, Inc. System, method, and product for scanning of biological materials
US20030186251A1 (en) 2002-04-01 2003-10-02 Brookhaven Science Associates, Llc Genome sequence tags
US20050175993A1 (en) 2002-04-12 2005-08-11 Chia-Lin Wei Method for making full-length coding sequence cDNA libraries
US20040049354A1 (en) 2002-04-26 2004-03-11 Affymetrix, Inc. Method, system and computer software providing a genomic web portal for functional analysis of alternative splice variants
US20040126840A1 (en) 2002-12-23 2004-07-01 Affymetrix, Inc. Method, system and computer software for providing genomic ontological data
US20070178478A1 (en) 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US6808906B2 (en) 2002-05-08 2004-10-26 Rigel Pharmaceuticals, Inc. Directionally cloned random cDNA expression vector libraries, compositions and methods of use
US20070065816A1 (en) 2002-05-17 2007-03-22 Affymetrix, Inc. Methods for genotyping
GB0212391D0 (en) 2002-05-29 2002-07-10 Axis Shield Asa Assay
US20050019776A1 (en) * 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
AU2003256285A1 (en) 2002-06-28 2004-01-19 Igen International, Inc. Improved assay systems and components
US7192700B2 (en) 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
WO2004018623A2 (en) 2002-08-16 2004-03-04 Clinical Microarrays, Inc. Substrates for isolating, reacting and microscopically analyzing materials
EP3067429A1 (en) 2002-09-03 2016-09-14 Quanta Biosciences, Inc. Improved methods for cdna synthesis
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
JP4201203B2 (en) 2002-09-30 2008-12-24 エフ．ホフマン−ラ ロシュ アーゲー Oligonucleotides for genotyping of the thymidylate synthase gene
JP5695287B2 (en) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー Nucleic acid analysis of microfluids
US7822555B2 (en) 2002-11-11 2010-10-26 Affymetrix, Inc. Methods for identifying DNA copy number changes
AU2003291481A1 (en) 2002-11-11 2004-06-03 Affymetrix, Inc. Methods for identifying dna copy number changes
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
WO2004066185A1 (en) 2003-01-23 2004-08-05 U.S. Genomics, Inc. Methods for analyzing polymer populations
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
WO2004081225A2 (en) 2003-03-07 2004-09-23 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
WO2006102264A1 (en) 2005-03-18 2006-09-28 Fluidigm Corporation Thermal reaction device and method for using the same
US7269518B2 (en) 2003-04-30 2007-09-11 Agilent Technologies, Inc. Chemical array reading
US20040259118A1 (en) 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
US20050042654A1 (en) 2003-06-27 2005-02-24 Affymetrix, Inc. Genotyping methods
CA2531105C (en) 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
US20060281092A1 (en) 2003-07-24 2006-12-14 Tanja Wille Method for the reverse transcription and/or amplification of nucleic acids
US7317415B2 (en) 2003-08-08 2008-01-08 Affymetrix, Inc. System, method, and product for scanning of biological materials employing dual analog integrators
WO2005017206A1 (en) 2003-08-13 2005-02-24 Affymetrix, Inc. Methods and kits for preparing nucleic acid samples
GB0319332D0 (en) 2003-08-16 2003-09-17 Astrazeneca Ab Amplification
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7341837B2 (en) 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
EP1660674B1 (en) 2003-09-10 2010-03-17 Althea Technologies, Inc. Expression profiling using microarrays
JP2007512811A (en) 2003-11-10 2007-05-24 インベスチゲン， インコーポレイテッド Methods for preparing nucleic acids for detection
JP2007524410A (en) 2004-01-23 2007-08-30 リングヴィテ エーエス Improved polynucleotide ligation reaction
JP2007521833A (en) 2004-02-12 2007-08-09 コンパス ジェネティクス エルエルシー Genetic analysis by sequence-specific classification
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
ES2527426T3 (en) 2004-05-07 2015-01-23 Cepheid Combined detection of biological agents
WO2005111242A2 (en) 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
CA2569745A1 (en) 2004-06-07 2005-12-22 Irm Llc Dispensing systems, software, and related methods
US20060035258A1 (en) 2004-08-06 2006-02-16 Affymetrix, Inc. Methods for identifying DNA copy number changes
US20060041385A1 (en) 2004-08-18 2006-02-23 Bauer Kenneth D Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization
US7713697B2 (en) 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
DE602005022740D1 (en) 2004-09-10 2010-09-16 Human Genetic Signatures Pty AMPLIFICATION BLOCKERS COMPRISING INTERCALING NUCLEIC ACIDS (INA) CONTAINING INTERCALING PSEUDONUCLEOTIDES (IPN)
EP1647600A3 (en) 2004-09-17 2006-06-28 Affymetrix, Inc. (A US Entity) Methods for identifying biological samples by addition of nucleic acid bar-code tags
US7435084B2 (en) 2004-12-14 2008-10-14 Align Technology, Inc. System and methods for casting physical tooth model
EP1842226B2 (en) 2004-11-03 2014-07-02 Iris International, Inc. Homogeneous analyte detection
EP1871527B1 (en) 2004-12-23 2017-09-27 Abbott Point of Care Inc. Molecular diagnostics system
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2006085616A1 (en) 2005-02-10 2006-08-17 Riken Method for amplification of nucleotide sequence
US20060211030A1 (en) 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
EP1871913A2 (en) 2005-03-25 2008-01-02 Ambion, Inc. Methods and compositions for depleting abundant rna transcripts
US7695886B2 (en) 2005-05-19 2010-04-13 Fuji Xerox Co., Ltd. Process for producing resin particle liquid dispersion for electrostatic image developing toner, electrostatic image developing toner and production process thereof
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US20070065844A1 (en) 2005-06-08 2007-03-22 Massachusetts Institute Of Technology Solution-based methods for RNA expression profiling
US7796815B2 (en) 2005-06-10 2010-09-14 The Cleveland Clinic Foundation Image analysis of biological objects
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
US7745092B2 (en) 2005-09-13 2010-06-29 Affymetrix, Inc. Multiple step printing methods for microbarcodes
WO2007035742A2 (en) 2005-09-16 2007-03-29 454 Life Sciences Corporation Cdna library preparation
US8831887B2 (en) 2005-10-12 2014-09-09 The Research Foundation For The State University Of New York Absolute PCR quantification
US8313711B2 (en) 2005-11-01 2012-11-20 Freeslate, Inc. Liquid dispensing for high-throughput experimentation
US7375211B2 (en) * 2005-11-18 2008-05-20 Kou Zhong C Method for detection and quantification of T-cell receptor Vβ repertoire
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
US7636465B2 (en) 2005-12-09 2009-12-22 Cytyc Corporation Cross-frame object reconstruction for image-based cytology applications
US7537897B2 (en) * 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
EP1987162A4 (en) 2006-01-23 2009-11-25 Population Genetics Technologi Nucleic acid analysis using sequence tokens
US8460878B2 (en) 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for detecting cells and cellular components in small defined volumes
US20080038727A1 (en) 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
US7880142B2 (en) 2006-04-04 2011-02-01 Sidec Technologies Ab Extended electron tomography
US20080194414A1 (en) 2006-04-24 2008-08-14 Albert Thomas J Enrichment and sequence analysis of genomic regions
WO2007136874A2 (en) 2006-05-18 2007-11-29 President And Fellows Of Harvard College Genomic library construction
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2007147079A2 (en) 2006-06-14 2007-12-21 Living Microsystems, Inc. Rare cell analysis using sample splitting and dna tags
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
CA2660286A1 (en) 2006-08-09 2008-02-21 Homestead Clinical Corporation Organ-specific proteins and methods of their use
US20100159590A1 (en) 2006-10-05 2010-06-24 Nanopoint, Inc. Systems and methods for active microfluidic cell handling
US8367051B2 (en) 2006-10-09 2013-02-05 Carnegie Mellon University Preparation of functional gel particles with a dual crosslink network
JP4896150B2 (en) 2006-10-18 2012-03-14 大崎電気工業株式会社 Electronic energy meter
WO2008051928A2 (en) 2006-10-23 2008-05-02 The Salk Institute For Biological Studies Target-oriented whole genome amplification of nucliec acids
DK2518162T3 (en) 2006-11-15 2018-06-18 Biospherex Llc Multi-tag sequencing and ecogenomic analysis
US8050476B2 (en) 2006-11-22 2011-11-01 General Electric Company Heart rate demodulation of periodic movement in ultrasound data for object identification
CA2672315A1 (en) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
EP2096429A4 (en) 2007-01-16 2009-12-16 Olympus Corp Fluorescent signal analyzing apparatus and fluorescent signal analyzing method
US9063133B2 (en) 2007-01-30 2015-06-23 The Regents Of The University Of California Methods and devices for biomolecular arrays
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
WO2008096318A2 (en) 2007-02-09 2008-08-14 Koninklijke Philips Electronics N.V. Identification system
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
CN101669031A (en) 2007-02-27 2010-03-10 森托科隆股份公司 Multiplex detection of tumour cells using a panel of agents binding to extracellular markers
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
KR100882711B1 (en) * 2007-03-12 2009-02-06 성균관대학교산학협력단 Uracil-DNA glycosylase of Psychrobacter sp. HJ147 and use thereof
JP2008256428A (en) 2007-04-03 2008-10-23 Intec Systems Institute Inc Method for detecting block position of microarray image
US20080268508A1 (en) 2007-04-30 2008-10-30 Sowlay Mohankumar R Methods and kits for negative selection of desired nucleic acid sequences
US20080274458A1 (en) 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US20090061513A1 (en) 2007-05-15 2009-03-05 Picovitro Ab Cell sorting and cell cultivation methods
JP2010528608A (en) 2007-06-01 2010-08-26 ４５４ ライフ サイエンシーズ コーポレイション System and method for identifying individual samples from complex mixtures
CN102083575B (en) 2007-06-25 2014-09-17 阿费梅特里克斯公司 Patterned microcodes
EP2395113A1 (en) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
DK2036989T3 (en) 2007-09-12 2012-10-22 Pasteur Institut Polynucleotide Suitable for Single Cell Based Reporter Assay for Monitoring Gene Expression Patterns with High Spatiotemporal Resolution
EP3136103B1 (en) 2007-10-05 2018-08-29 Affymetrix, Inc. Highly multiplexed assays
US20090253586A1 (en) 2008-02-21 2009-10-08 Gentel Biosciences, Inc. Substrates for multiplexed assays and uses thereof
US8687189B2 (en) 2008-03-03 2014-04-01 Ajjer, Llc Analysis of arrays by laser induced breakdown spectroscopy
US8206925B2 (en) 2008-04-14 2012-06-26 Medical Diagnostic Laboratories, Llc Splice variants of human IL-23 receptor (IL-23R) mRNA and use of a delta 9 isoform in predicting inflammatory bowel diseases
DE102008025656B4 (en) * 2008-05-28 2016-07-28 Genxpro Gmbh Method for the quantitative analysis of nucleic acids, markers therefor and their use
US20090298709A1 (en) 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
WO2009148560A2 (en) 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Methods and compositions for nucleic acid sequencing
US20100069250A1 (en) 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8835110B2 (en) 2008-11-04 2014-09-16 The Johns Hopkins University DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
CN102317779A (en) 2008-11-21 2012-01-11 圣路易大学 Biosensor for detecting multiple epitopes on a target
US20100159533A1 (en) 2008-11-24 2010-06-24 Helicos Biosciences Corporation Simplified sample preparation for rna analysis
US8492096B2 (en) 2008-12-24 2013-07-23 Boris Pasche TGFBR1 expression modifies risk for colorectal cancer
EP3020830A1 (en) * 2009-01-20 2016-05-18 The Board Of Trustees Of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
US8715928B2 (en) * 2009-02-13 2014-05-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Molecular-based method of cancer diagnosis and prognosis
ES2617191T3 (en) 2009-03-05 2017-06-15 Daiichi Sankyo Company, Limited Pyridine derivative as a PPARY inhibitor
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
DK3495498T3 (en) * 2009-03-30 2022-01-17 Illumina Inc GENE EXPRESSION ANALYSIS IN SINGLE CELLS
DK2414547T3 (en) 2009-04-02 2014-06-16 Fluidigm Corp Multiprimer amplification method for bar coding of target nucleic acids
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
CN102428180A (en) 2009-05-14 2012-04-25 和光纯药工业株式会社 Method for synthesis of double-stranded DNA corresponding to RNA, and method for amplification of the DNA
FR2945545B1 (en) 2009-05-14 2011-08-05 Univ Aix Marseille Ii METHOD FOR DETECTION OF PROCARYOTE DNA EXTRACTED FROM A SAMPLE SAMPLE
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
US8330957B2 (en) 2009-06-29 2012-12-11 Hager Enviromental and Atmospheric Technologies, LLC Device and method for quantification of gases in plumes by remote sensing
JP5785942B2 (en) * 2009-06-29 2015-09-30 ルミネックス コーポレーション Chimeric primer with hairpin conformation and use thereof
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
RU2552215C2 (en) 2009-09-01 2015-06-10 Конинклейке Филипс Электроникс Н.В. Device and method of selecting nucleic acids by means of micro-arrays
WO2011028818A2 (en) 2009-09-01 2011-03-10 Trustees Of Boston University High throughput multichannel reader and uses thereof
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
WO2011041308A1 (en) 2009-09-30 2011-04-07 Quest Diagnostics Investments Incorporated Bcr-abl truncation mutations
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
AU2010343276B2 (en) 2010-01-19 2015-05-28 Verinata Health, Inc. Methods for determining fraction of fetal nucleic acid in maternal samples
WO2011091046A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing
EP2529026B1 (en) 2010-01-25 2013-11-13 Rd Biosciences Inc. Self-folding amplification of target nucleic acid
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
JP5901046B2 (en) 2010-02-19 2016-04-06 国立大学法人 千葉大学 Novel alternative splicing variant of OATP1B3 mRNA
WO2011106738A2 (en) 2010-02-25 2011-09-01 Fred Hutchinson Cancer Research Center Use of tcr clonotypes as biomarkers for disease
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
ES2555106T3 (en) 2010-04-05 2015-12-29 Prognosys Biosciences, Inc. Spatially coded biological assays
US20140227691A1 (en) 2010-05-14 2014-08-14 Fluidigm, Inc. Nucleic acid isolation methods
US8828688B2 (en) * 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
ES2960184T3 (en) 2010-06-09 2024-03-01 Keygene Nv Combinatorial Sequence Barcoding for High-Throughput Screening
US20120004132A1 (en) 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
KR101374465B1 (en) 2010-07-07 2014-03-18 가부시키가이샤 어드밴티스트 Testing device and testing method
EP2407242A1 (en) 2010-07-13 2012-01-18 Dublin City University Direct clone analysis and selection technology
US20120040843A1 (en) 2010-08-12 2012-02-16 Dublin City University Centrifugal capture system
WO2012027503A2 (en) 2010-08-24 2012-03-01 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
EP3115468B1 (en) 2010-09-21 2018-07-25 Agilent Technologies, Inc. Increasing confidence of allele calls with molecular counting
EP2436766A1 (en) 2010-09-29 2012-04-04 Deutsches Krebsforschungszentrum Means and methods for improved protein interaction screening
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
GB2497912B (en) 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
US20120088691A1 (en) 2010-10-08 2012-04-12 Gao Chen Highly multiplexed real-time pcr using encoded microbeads
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
AU2011336707A1 (en) 2010-11-29 2013-07-04 Dako Denmark A/S Methods and systems for analyzing images of specimens processed by a programmable quantitative assay
US9394511B2 (en) 2010-12-05 2016-07-19 Wenbin Jiang Rapid single cell based parallel biological cell sorter
CN103403545B (en) 2010-12-09 2018-06-22 阿科尼生物***公司 Sample analysis system
US20140057799A1 (en) 2010-12-16 2014-02-27 Gigagen System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
ES2770342T3 (en) * 2010-12-22 2020-07-01 Natera Inc Noninvasive Prenatal Paternity Testing Procedures
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
EP2665833B1 (en) 2011-01-17 2017-04-19 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
JP6069224B2 (en) 2011-01-31 2017-02-01 アプライズ バイオ， インコーポレイテッド Methods for identifying multiple epitopes in a cell
WO2016100976A2 (en) 2014-12-19 2016-06-23 Apprise Bio, Inc. Methods for identifying multiple epitopes in selected sub-populations of cells
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
AU2012220596B2 (en) 2011-02-23 2016-05-12 Board Of Regents Use of template switching for DNA synthesis
US9260753B2 (en) * 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
KR20120110431A (en) 2011-03-29 2012-10-10 에스케이하이닉스 주식회사 A semiconductor memory apparatus
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2697397B1 (en) 2011-04-15 2017-04-05 The Johns Hopkins University Safe sequencing system
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
EP3395957B1 (en) 2011-04-25 2020-08-12 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP3421591B1 (en) 2011-04-28 2023-09-27 The Board of Trustees of the Leland Stanford Junior University Identification of polynucleotides associated with a sample
GB201108259D0 (en) 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
PT3892295T (en) 2011-05-24 2023-06-01 Tron Translationale Onkologie An Der Univ Der Johannes Gutenberg Univ Mainz Gemeinnuetzige Gmbh Individualized vaccines for cancer
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
WO2012177639A2 (en) 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
WO2013003498A2 (en) 2011-06-27 2013-01-03 Life Technologies Corporation Acoustic cytometry methods and protocols
KR101454886B1 (en) 2011-08-01 2014-11-03 주식회사 셀레믹스 Method for synthesizing nucleic acid molecules
WO2013022961A1 (en) 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
JP5816486B2 (en) 2011-08-18 2015-11-18 オリンパス株式会社 Fluorescence observation apparatus, fluorescence observation system, and fluorescence image processing method of fluorescence observation apparatus
LT2756098T (en) 2011-09-16 2018-09-10 Lexogen Gmbh Method for making library of nucleic acid molecules
WO2013062931A2 (en) 2011-10-24 2013-05-02 Furchak Jennifer A molecular beacon based assay for the detection of biomarkers of breast cancer metastasis
ES2884100T3 (en) 2011-11-11 2021-12-10 Eli N Glezer Coagent-Assisted Binding Assay Procedures
KR101337094B1 (en) 2011-11-30 2013-12-05 삼성에스디에스 주식회사 Apparatus and method for sequence alignment
EP3211100A1 (en) 2011-12-22 2017-08-30 Ibis Biosciences, Inc. Amplification primers and methods
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP3495817A1 (en) 2012-02-10 2019-06-12 Raindance Technologies, Inc. Molecular diagnostic screening assay
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
EP3309262B1 (en) 2012-02-24 2019-09-25 Bio-Rad Laboratories, Inc. Labeling and sample preparation for sequencing
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
ES2776673T3 (en) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Methods and uses for molecular tags
WO2013137737A1 (en) 2012-03-16 2013-09-19 Flexgen B.V. Methods of creating and screening of dna encoded combinatorial libraries of chemical antibodies
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
WO2013169957A1 (en) 2012-05-08 2013-11-14 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
CA2873585C (en) 2012-05-14 2021-11-09 Cb Biotechnologies, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
JP2015519900A (en) 2012-05-21 2015-07-16 フリューダイム・コーポレイション Single particle analysis method and single particle isolation method for particle population
EP2856177B1 (en) 2012-05-25 2020-11-18 The University of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
EP2861760B1 (en) 2012-06-15 2020-01-01 The Board of Regents of The University of Texas System High throughput sequencing of multiple transcripts of a single cell
SG11201408128WA (en) 2012-06-15 2015-01-29 Adaptive Biotechnologies Corp Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
JP6181751B2 (en) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド Compositions and methods for negative selection of unwanted nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2875173B1 (en) 2012-07-17 2017-06-28 Counsyl, Inc. System and methods for detecting genetic variation
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
CA2878694A1 (en) 2012-07-24 2014-01-30 Sequenta, Inc. Single cell analysis using sequence tags
EP3428290B1 (en) 2012-07-26 2022-04-06 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
EP3578669A1 (en) 2012-08-08 2019-12-11 F. Hoffmann-La Roche AG Increasing dynamic range for identifying multiple epitopes in cells
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
CN114891871A (en) 2012-08-14 2022-08-12 10X基因组学有限公司 Microcapsule compositions and methods
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2888349B1 (en) 2012-08-24 2022-02-09 Yale University System, device and method for high-throughput multi-plexed detection
CA2886974C (en) 2012-10-17 2021-06-29 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
CA2889862C (en) 2012-11-05 2021-02-16 Rubicon Genomics, Inc. Barcoding nucleic acids
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
US9562269B2 (en) 2013-01-22 2017-02-07 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of HLA loci with ultra-deep shotgun sequencing
CN104955576A (en) 2013-01-24 2015-09-30 沙特基础全球技术有限公司 Microwell plate made from a polyester-polycarbonate
US10017761B2 (en) 2013-01-28 2018-07-10 Yale University Methods for preparing cDNA from low quantities of cells
CN104969056B (en) 2013-02-01 2018-05-01 贝克顿迪金森公司 Assess the method and system of sample behavior in flow cytometer
CN105102696B (en) 2013-02-08 2017-08-15 生物辐射实验室股份有限公司 The experiment of the division based on affinity detected for target molecule
EP3862435A1 (en) 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
WO2014126937A1 (en) 2013-02-14 2014-08-21 The Board Of Trustees Of The Leland Stanford Junior University Suspension arrays and multiplexed assays based thereon
US9621600B2 (en) 2013-02-26 2017-04-11 PortAura Group Method and system for providing recommendations using location information
US20140274811A1 (en) 2013-03-14 2014-09-18 Lyle J. Arnold Methods for Amplifying a Complete Genome or Transcriptome
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
CA2906076A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
CN105189748B (en) 2013-03-15 2021-06-08 血统生物科学公司 Method for sequencing an immune repertoire
US9938523B2 (en) 2013-03-15 2018-04-10 Arizona Board Of Regents On Behalf Of Arizona State University Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014165762A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
WO2014176575A1 (en) 2013-04-25 2014-10-30 Firefly Bioworks, Inc. Multiplexed analysis of target nucleic acids
WO2014179735A1 (en) 2013-05-03 2014-11-06 Cambrian Genomics, Inc. Method and apparatus for producing sequence verified dna
EP2805769A1 (en) 2013-05-24 2014-11-26 European Molecular Biology Laboratory Methods for nano-scale single cell analysis
JP6600302B2 (en) 2013-06-12 2019-10-30 ザ ジェネラル ホスピタル コーポレイション Methods, kits, and systems for multiplex detection of target molecules and uses thereof
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
WO2014204939A2 (en) 2013-06-17 2014-12-24 Kim Lewis Methods for quantitative determination of protein-nucleic acid interactions in complex mixtures
DK3013983T3 (en) 2013-06-25 2023-03-06 Prognosys Biosciences Inc SPATIALLY ENCODED BIOLOGICAL ASSAYS USING A MICROFLUIDIC DEVICE
JP6563912B2 (en) 2013-06-27 2019-08-21 テンエックス・ジェノミクス・インコーポレイテッド Compositions and methods for sample processing
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
WO2015006503A1 (en) 2013-07-09 2015-01-15 Smith Lucas David Device and method of rapid linker mediated label-based immunoassays
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN105683397A (en) 2013-09-04 2016-06-15 富鲁达公司 Proximity assays for detecting nucleic acids and proteins in a single cell
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
EP3052658B1 (en) 2013-09-30 2021-03-31 Vesicode AB Methods to profile molecular complexes by using proximity dependant bar-coding
JP2017504307A (en) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド Method and system for digitally counting features on an array
EP3572527B1 (en) 2013-10-17 2021-05-26 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
US9709479B2 (en) 2013-10-25 2017-07-18 Massachusetts Institute Of Technology Method and apparatus for tracking cell identity
WO2015061844A1 (en) 2013-10-30 2015-05-07 The University Of Sydney Assay to stratify cancer patients
WO2015070037A2 (en) 2013-11-08 2015-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
SG10201807112XA (en) 2013-12-30 2018-09-27 Atreca Inc Analysis of nucleic acids associated with single cells using nucleic acid barcodes
CA2938080A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
US20150218620A1 (en) 2014-02-03 2015-08-06 Integrated Dna Technologies, Inc. Methods to capture and/or remove highly abundant rnas from a heterogenous rna sample
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
ES2777529T3 (en) 2014-04-17 2020-08-05 Adaptive Biotechnologies Corp Quantification of adaptive immune cell genomes in a complex mixture of cells
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
JP6412954B2 (en) 2014-04-29 2018-10-24 イルミナ インコーポレイテッド Multiple analysis of single cell gene expression using template switching and tagging
BR112016027063A2 (en) 2014-05-19 2017-10-31 Univ Rice William M allele-specific amplification using a non-allele-specific primer overlap composition and allele-specific blocking oligonucleotides
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
AU2015279548B2 (en) 2014-06-26 2020-02-27 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
AU2015279617A1 (en) 2014-06-26 2017-01-12 10X Genomics, Inc. Analysis of nucleic acid sequences
US20160017320A1 (en) 2014-07-15 2016-01-21 Qiagen Sciences, Llc Semi-random barcodes for nucleic acid analysis
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
SG10201911069WA (en) 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
US9529672B2 (en) 2014-09-25 2016-12-27 Everspin Technologies Inc. ECC word configuration for system-level ECC compatibility
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
EP3248018B1 (en) 2015-01-22 2020-01-08 Becton, Dickinson and Company Devices and systems for molecular barcoding of nucleic acid targets in single cells
WO2016118719A1 (en) 2015-01-23 2016-07-28 Qiagen Sciences, Llc High multiplex pcr with molecular barcoding
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
CN107208156B (en) 2015-02-09 2021-10-08 10X基因组学有限公司 System and method for determining structural variation and phasing using variation recognition data
EP3766988B1 (en) 2015-02-19 2024-02-14 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
CN107208157B (en) 2015-02-27 2022-04-05 贝克顿迪金森公司 Methods and compositions for barcoding nucleic acids for sequencing
CN107208158B (en) 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
WO2016145409A1 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Genotype and phenotype coupling
US20160266094A1 (en) 2015-03-13 2016-09-15 University Of Iowa Research Foundation Cellular barcode
WO2016149418A1 (en) 2015-03-18 2016-09-22 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
WO2016160965A1 (en) 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Methods and compositions for repair of dna ends by multiple enzymatic activities
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016168825A1 (en) 2015-04-17 2016-10-20 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US9618871B2 (en) 2015-04-28 2017-04-11 Kyocera Document Solutions Inc. Image forming apparatus
WO2016191272A1 (en) 2015-05-22 2016-12-01 Sigma-Aldrich Co. Llc Methods for next generation genome walking and related compositions and kits
EP3303587A4 (en) 2015-05-28 2018-11-21 Kaarel Krjutskov Blocking oligonucleotides
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
CN107849561A (en) 2015-08-24 2018-03-27 凯杰有限公司 The method for producing RNA sequencing libraries
EP4086357A1 (en) 2015-08-28 2022-11-09 Illumina, Inc. Nucleic acid sequence analysis from single cells
US10619186B2 (en) 2015-09-11 2020-04-14 Cellular Research, Inc. Methods and compositions for library normalization
EP3933047A1 (en) 2015-09-24 2022-01-05 AbVitro LLC Affinity-oligonucleotide conjugates and uses thereof
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
WO2017079593A1 (en) 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US20170136458A1 (en) 2015-11-18 2017-05-18 Wafergen, Inc. Systems and methods for pooling samples from multi-well devices
WO2017096239A1 (en) 2015-12-04 2017-06-08 St. Jude Children's Research Hospital, Inc. Cloning and expression system for t-cell receptors
EP3397764A4 (en) 2015-12-30 2019-05-22 Bio-Rad Laboratories, Inc. Digital protein quantification
US20170205404A1 (en) 2016-01-19 2017-07-20 General Electric Company Multifunctional beads and methods of use for capturing rare cells
WO2017139690A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
WO2017164936A1 (en) 2016-03-21 2017-09-28 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells
CN109312331B (en) 2016-04-01 2022-08-02 贝勒医学院 Method for whole transcriptome amplification
AU2017261189B2 (en) 2016-05-02 2023-02-09 Becton, Dickinson And Company Accurate molecular barcoding
WO2017196768A1 (en) 2016-05-09 2017-11-16 President And Fellows Of Harvard College Self-targeting guide rnas in crispr system
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
JP7046007B2 (en) 2016-05-26 2022-04-01 ベクトン・ディキンソン・アンド・カンパニー How to adjust the molecular label count
TWI600309B (en) 2016-05-28 2017-09-21 Hon Hai Prec Ind Co Ltd Angle adjusting mechanism
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
LT3263715T (en) 2016-06-28 2020-06-25 Hifibio Method for transcriptome analysis of single cells
WO2018017949A1 (en) 2016-07-22 2018-01-25 Verily Life Sciences Llc Quantitative massively parallel proteomics
WO2018020489A1 (en) 2016-07-24 2018-02-01 Yeda Research And Development Co. Ltd. Methods and kits for analyzing dna binding moieties attached to dna
US20180037942A1 (en) 2016-08-03 2018-02-08 Cellular Research, Inc. Enzyme-independent molecular indexing
CA3033506A1 (en) 2016-08-10 2018-02-15 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic dna fragments
JP7091348B2 (en) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP3521425A4 (en) 2016-09-29 2019-10-23 FUJIFILM Corporation Method for designing primers for multiplex pcr
EP3521424A4 (en) 2016-09-29 2019-10-23 FUJIFILM Corporation Method for designing primers for multiplex pcr
KR102650753B1 (en) 2016-10-01 2024-03-26 버클리 라잇츠, 인크. DNA barcode compositions and methods for in situ identification in microfluidic devices
DK3529357T3 (en) 2016-10-19 2022-04-25 10X Genomics Inc Methods for bar coding nucleic acid molecules from individual cells
WO2018090298A2 (en) 2016-11-17 2018-05-24 Genomicare Biotechnology (Shanghai) Co. Ltd. Systems and methods for monitoring lifelong tumor evolution
US20180163201A1 (en) 2016-12-12 2018-06-14 Grail, Inc. Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
CA3046007A1 (en) 2016-12-22 2018-06-28 Guardant Health, Inc. Methods and systems for analyzing nucleic acid molecules
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
CA3050127A1 (en) 2017-01-12 2018-07-19 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
US20180208975A1 (en) 2017-01-20 2018-07-26 Merck Sharp & Dohme Corp. Assay for simultaneous genomic and proteomic analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
CN110475864B (en) 2017-02-02 2024-01-12 纽约基因组研究中心公司 Methods and compositions for identifying or quantifying targets in biological samples
WO2018170515A1 (en) 2017-03-17 2018-09-20 The Broad Institute, Inc. Methods for identifying and modulating co-occurant cellular phenotypes
JP7169290B2 (en) 2017-03-24 2022-11-10 ベクトン・ディキンソン・アンド・カンパニー Composite multiplets for determining multiplets
CN110462058A (en) 2017-03-24 2019-11-15 新加坡国立大学 Method for Multiple detection molecule
CN111051526A (en) 2017-05-23 2020-04-21 生捷科技控股公司 Method for performing analysis of spatial distribution of biomolecules
CA3064709A1 (en) 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
MA49352A (en) 2017-05-26 2020-04-08 Abvitro Llc HIGH YIELD POLYNUCLEOTIDE LIBRARY SEQUENCING AND TRANSCRIPTOME ANALYSIS
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
MX2019014264A (en) 2017-05-29 2020-01-23 Harvard College A method of amplifying single cell transcriptome.
EP3635135A1 (en) 2017-06-05 2020-04-15 Becton, Dickinson and Company Sample indexing for single cells
WO2019055852A1 (en) 2017-09-15 2019-03-21 Apton Biosystems, Inc. Heterogeneous single cell profiling using molecular barcoding
CN111247589A (en) 2017-09-25 2020-06-05 贝克顿迪金森公司 Immune receptor barcode error correction
DK4060041T3 (en) 2017-09-25 2024-04-02 Fred Hutchinson Cancer Center HIGH EFFICIENCY TARGETED IN SITU GENOME-WIDE PROFILING
WO2019076768A1 (en) 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As Method and kit for dna library preparation
US11702649B2 (en) 2017-10-23 2023-07-18 The Broad Institute, Inc. Single cell cellular component enrichment from barcoded sequencing libraries
WO2019099906A1 (en) 2017-11-16 2019-05-23 The Regents Of The University Of Colorado, A Body Corporate Methods to measure functional heterogeneity among single cells
US20200362334A1 (en) 2017-12-07 2020-11-19 The Broad Institute, Inc. High-throughput methods for identifying gene interactions and networks
EP3720605A1 (en) 2017-12-07 2020-10-14 Massachusetts Institute Of Technology Single cell analyses
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
CN114807306A (en) 2017-12-08 2022-07-29 10X基因组学有限公司 Methods and compositions for labeling cells
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
CN112020565A (en) 2018-01-05 2020-12-01 十亿至一公司 Quality control template for ensuring validity of sequencing-based assays
WO2019152108A1 (en) 2018-02-05 2019-08-08 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
EP3765596A4 (en) 2018-03-12 2022-04-06 Silicon Valley Scientific, Inc. Method and apparatus for processing tissue and other samples encoding cellular spatial position information
WO2019204560A1 (en) 2018-04-18 2019-10-24 The Regents Of The University Of California Method to connect chromatin accessibility and transcriptome
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
ES2945191T3 (en) 2018-05-03 2023-06-29 Becton Dickinson Co High-throughput multi-omics sample analysis
CN110499251A (en) 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 The extracellular vesica analysis chip of the unicellular source property of high flux multiparameter and application
CN112689673A (en) 2018-07-11 2021-04-20 埃克斯基因美国公司 Transposome-enabled DNA/RNA sequencing (TED RNA-SEQ)
JP7413351B2 (en) 2018-08-03 2024-01-15 ベクトン・ディキンソン・アンド・カンパニー Nuclear barcoding and capture in single cells
CA3108770A1 (en) 2018-08-08 2020-02-13 Vanderbilt University Systems and methods for simultaneous detection of antigens and antigen specific antibodies
EP4242324A3 (en) 2018-08-17 2023-09-27 Becton, Dickinson and Company Aptamer barcoding
EP3844299A1 (en) 2018-08-28 2021-07-07 Becton Dickinson and Company Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
CA3123847A1 (en) 2018-12-17 2020-06-25 Natera, Inc. Methods for analysis of circulating cells
ES2945227T3 (en) 2019-01-23 2023-06-29 Becton Dickinson Co Antibody Associated Oligonucleotides
EP3918090A4 (en) 2019-01-28 2022-10-26 Becton, Dickinson and Company Oligonucleotide-comprising cellular component-binding reagents and methods of using the same
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
SG10201904897UA (en) 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
EP3997217A4 (en) 2019-07-12 2023-06-28 New York Genome Center, Inc. Methods and compositions for scalable pooled rna screens with single cell chromatin accessibility profiling
US20210039582A1 (en) 2019-08-07 2021-02-11 ZF Passive Safety Systems US Inc. Airbag package with improved packaging
JP2023500679A (en) 2019-11-08 2023-01-10 ベクトン・ディキンソン・アンド・カンパニー Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
WO2021142233A1 (en) 2020-01-10 2021-07-15 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
CN115279919A (en) 2020-01-13 2022-11-01 贝克顿迪金森公司 Cell capture using DU-containing oligonucleotides
US20210222244A1 (en) 2020-01-17 2021-07-22 Becton, Dickinson And Company Methods and compositions for single cell secretomics
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US20210238661A1 (en) 2020-01-31 2021-08-05 Becton, Dickinson And Company Mesophilic dna polymerase extension blockers
US20210246492A1 (en) 2020-02-12 2021-08-12 Becton, Dickinson And Company Intracellular abseq
WO2021168015A1 (en) 2020-02-17 2021-08-26 Universal Sequencing Technology Corporation Methods of barcoding nucleic acid for detection and sequencing
CN116157533A (en) 2020-02-21 2023-05-23 10X基因组学有限公司 Capturing genetic targets using hybridization methods
WO2021178199A1 (en) 2020-03-05 2021-09-10 BioLegend, Inc. Methods and compositions for detecting targets
WO2021247593A1 (en) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
EP4179110A1 (en) 2020-07-13 2023-05-17 Becton, Dickinson and Company Cdna spike-in control for single cell analysis
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US20220033810A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
JP2023539822A (en) 2020-08-20 2023-09-20 ベクトン・ディキンソン・アンド・カンパニー Blood cell lysers for isolating bacteria from blood cultures
WO2022108946A1 (en) 2020-11-17 2022-05-27 Becton, Dickinson And Company Combined analysis of cell-free nucleic acids and single cells for oncology diagnostics
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
WO2022109339A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Use of dextramer in single cell analysis
EP4260063A1 (en) 2020-12-09 2023-10-18 Becton, Dickinson and Company Multiplexed single cell immunoassay
WO2022143221A1 (en) 2020-12-31 2022-07-07 中国科学院北京基因组研究所(国家生物信息中心) Method and kit for labeling nucleic acid molecules
JP2024502618A (en) 2021-01-08 2024-01-22 セラノーム， インコーポレイテッド Devices and methods for analyzing biological samples

Also Published As

Publication number Publication date
AU2018220004B2 (en) 2020-07-02
GB2513024B (en) 2016-08-31
AU2013226081B2 (en) 2018-06-14
JP6632596B2 (en) 2020-01-22
US20210230582A1 (en) 2021-07-29
US11634708B2 (en) 2023-04-25
CN104364392B (en) 2018-05-25
EP3321378B1 (en) 2021-11-10
JP2015511819A (en) 2015-04-23
WO2013130674A1 (en) 2013-09-06
CA2865575C (en) 2024-01-16
GB2504240B (en) 2015-05-27
ES2904816T3 (en) 2022-04-06
JP6375230B2 (en) 2018-08-15
CA3218231A1 (en) 2013-09-06
GB201408829D0 (en) 2014-07-02
JP2018064585A (en) 2018-04-26
CN104364392A (en) 2015-02-18
CA2865575A1 (en) 2013-09-06
EP3321378A1 (en) 2018-05-16
AU2013226081A1 (en) 2014-09-18
GB2513024A (en) 2014-10-15
GB2504240A (en) 2014-01-22
EP2820158A1 (en) 2015-01-07
HK1194109A1 (en) 2014-10-10
US10941396B2 (en) 2021-03-09
AU2018220004A1 (en) 2018-09-06
HK1206069A1 (en) 2015-12-31
EP2820158A4 (en) 2015-08-19
US20150133319A1 (en) 2015-05-14
ES2663234T3 (en) 2018-04-11
US20210238588A1 (en) 2021-08-05
GB201319347D0 (en) 2013-12-18
EP2820158B1 (en) 2018-01-10

Similar Documents

Publication Publication Date Title
HK1206069A1 (en) Compositions and kits for molecular counting
EP2836212A4 (en) Novel compositions and methods
EP2922861A4 (en) Biomarker compositions and methods
EP2823306A4 (en) Biomarker compositions and methods
ZA201408594B (en) Xylitol-based anti-mucosal compositions and related methods and compositions
HK1216250A1 (en) 8-hydroxy-dihydroergotamine compounds and compositions 8--
GB2506426B (en) Composition
GB201218954D0 (en) Composition
GB201315350D0 (en) Methods and compositions
HK1199198A1 (en) Composition
GB201315347D0 (en) Methods and compositions
EP2879670A4 (en) Antibacterial compositions and methods
EP2874641A4 (en) Glutathione-elevating compositions and uses thereof
EP2878295A4 (en) Composition
EP2931280A4 (en) Methods and compositions for inhibiting cnksr1
GB201219657D0 (en) Composition
GB201206035D0 (en) Composition
EP2910538A4 (en) Gas-generating-agent composition
EP2910537A4 (en) Gas-generating-agent composition
GB201208133D0 (en) Composition
IL239426A0 (en) Biomarker methods and compositions
EP2911002A4 (en) Resist composition
GB201311517D0 (en) Composition
GB201219383D0 (en) Composition
GB201218195D0 (en) Composition