DE60037508D1 - Verfahren zur herstellung von rekombinantem insulin durch fusionsproteine - Google Patents

Verfahren zur herstellung von rekombinantem insulin durch fusionsproteine

Info

Publication number
DE60037508D1
DE60037508D1 DE60037508T DE60037508T DE60037508D1 DE 60037508 D1 DE60037508 D1 DE 60037508D1 DE 60037508 T DE60037508 T DE 60037508T DE 60037508 T DE60037508 T DE 60037508T DE 60037508 D1 DE60037508 D1 DE 60037508D1
Authority
DE
Germany
Prior art keywords
amino acid
acid sequence
chain
insulin
fusion proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60037508T
Other languages
English (en)
Other versions
DE60037508T2 (de
Inventor
Shusaku Oka
Seiji Sato
Naohiko Higashikuni
Masaaki Kondo
Toshiyuki Kudo
Shigeaki Watanabe
Yoshihiro Waki
Hirotaka Yuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Itoham Foods Inc
Original Assignee
Itoham Foods Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Itoham Foods Inc filed Critical Itoham Foods Inc
Application granted granted Critical
Publication of DE60037508D1 publication Critical patent/DE60037508D1/de
Publication of DE60037508T2 publication Critical patent/DE60037508T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60037508T 1999-04-30 2000-04-26 Verfahren zur herstellung von rekombinantem insulin durch fusionsproteine Expired - Fee Related DE60037508T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP12487799 1999-04-30
JP12487799A JP3406244B2 (ja) 1999-04-30 1999-04-30 新規な融合蛋白質からの組み換えインスリンの製造方法
PCT/JP2000/002736 WO2000066738A1 (fr) 1999-04-30 2000-04-26 Procede de production d'insuline recombinee a partir d'une nouvelle proteine fusionnee

Publications (2)

Publication Number Publication Date
DE60037508D1 true DE60037508D1 (de) 2008-01-31
DE60037508T2 DE60037508T2 (de) 2008-12-11

Family

ID=14896314

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60037508T Expired - Fee Related DE60037508T2 (de) 1999-04-30 2000-04-26 Verfahren zur herstellung von rekombinantem insulin durch fusionsproteine

Country Status (14)

Country Link
US (1) US6841361B1 (de)
EP (1) EP1179593B1 (de)
JP (1) JP3406244B2 (de)
KR (1) KR100659671B1 (de)
CN (1) CN1213146C (de)
AT (1) ATE381619T1 (de)
AU (1) AU775471B2 (de)
CA (1) CA2372221A1 (de)
DE (1) DE60037508T2 (de)
DK (1) DK1179593T3 (de)
EA (1) EA005586B1 (de)
HK (1) HK1042113A1 (de)
WO (1) WO2000066738A1 (de)
ZA (1) ZA200108951B (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1270730A4 (de) * 2000-03-14 2003-05-14 Itoham Foods Inc Verfahren zurherstellung eines polypeptids, welches eine disulfidbindung enthält
US7312192B2 (en) * 2001-09-07 2007-12-25 Biocon Limited Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
AU2003244053A1 (en) * 2003-06-03 2005-01-21 Shanghai Centre Of Research And Development Of New Drugs A fusion protein suitable to be expressed high effectively and the production method thereof
ES2546016T3 (es) * 2005-10-13 2015-09-17 Biocon Limited Procedimiento para la preparación de conjugados de insulina
WO2009101672A1 (ja) * 2008-02-12 2009-08-20 Itoham Foods Inc. 高発現分泌インスリン前駆体を含む融合タンパク質、それをコードするｄｎａおよびインスリンの製造方法
CN103917656B (zh) 2011-10-25 2016-09-28 味之素株式会社 蛋白质的分泌产生方法
KR102666154B1 (ko) 2018-08-08 2024-05-20 주식회사 대웅제약 지속형 인슐린 아날로그 및 그 복합체
KR102646845B1 (ko) * 2018-08-08 2024-03-14 주식회사 대웅제약 클로스트리파인을 이용한 지속형 인슐린 아날로그 복합체의 활성형 제조방법
CN113773400B (zh) * 2020-06-09 2023-08-18 宁波鲲鹏生物科技有限公司 一种门冬胰岛素衍生物及其应用
CN113773392B (zh) * 2020-06-09 2023-04-07 宁波鲲鹏生物科技有限公司 一种甘精胰岛素的制备方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4431740A (en) 1979-09-12 1984-02-14 The Regents Of The University Of California DNA Transfer vector and transformed microorganism containing human proinsulin and pre-proinsulin genes
NZ196610A (en) 1980-03-27 1984-03-16 Lilly Co Eli Preparation of insulin or insulin analog
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
JPH07108224B2 (ja) * 1985-11-07 1995-11-22 重三 鵜高 バチルス・ブレビスを用いる遺伝子の発現方法
JPS6387980A (ja) * 1986-09-30 1988-04-19 Shikishima Boseki Kk プラスミドベクタ−、菌株、及びそれを用いてペプシノ−ゲンを生産する方法
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
DE3936876A1 (de) * 1989-11-06 1991-05-23 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
JPH04126084A (ja) * 1990-05-11 1992-04-27 Hoechst Japan Ltd 蛋白質の製造法
US5378613A (en) * 1991-09-24 1995-01-03 Eli Lilly And Company Method for increased expression of low molecular weight recombinant polypeptides
EP0600372B1 (de) * 1992-12-02 1997-02-05 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
JP3164181B2 (ja) * 1993-03-01 2001-05-08 ヒゲタ醤油株式会社 新規発現ベクタ−、該発現ベクタ−を保有する微生物、該微生物を用いる有用物質の製造法
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
HRP940432B1 (en) * 1994-08-05 2003-10-31 Pliva Pharm & Chem Works Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin
BR9408638A (pt) 1994-12-29 2004-09-21 Bio Technology General Corp Geração de insulina humana
KR0150565B1 (ko) * 1995-02-15 1998-08-17 김정재 유전자 조환에 의한 사람 인슐린 전구체의 제조 및 이를 이용한 인슐린의 제조방법
FR2732978B1 (fr) * 1995-04-14 1997-05-30 Inst Nat Sante Rech Med Vecteur viral recombinant, composition pharmaceutique le contenant et cellules transformees correspondantes
GB9513967D0 (en) 1995-07-08 1995-09-06 Univ Leicester Insulin
JP3313083B2 (ja) * 1998-03-31 2002-08-12 伊藤ハム株式会社 新規な融合蛋白質をコ―ドするｄｎａおよびその発現を介する有用ポリペプチドの製造方法

Also Published As

Publication number Publication date
CA2372221A1 (en) 2000-11-09
EP1179593A1 (de) 2002-02-13
KR20020018192A (ko) 2002-03-07
KR100659671B1 (ko) 2006-12-21
EP1179593B1 (de) 2007-12-19
EA005586B1 (ru) 2005-04-28
AU775471B2 (en) 2004-08-05
US6841361B1 (en) 2005-01-11
HK1042113A1 (zh) 2002-08-02
JP2000316579A (ja) 2000-11-21
CN1350584A (zh) 2002-05-22
EP1179593A4 (de) 2003-04-16
DK1179593T3 (da) 2008-04-28
CN1213146C (zh) 2005-08-03
AU4142500A (en) 2000-11-17
DE60037508T2 (de) 2008-12-11
WO2000066738A1 (fr) 2000-11-09
JP3406244B2 (ja) 2003-05-12
EA200101161A1 (ru) 2002-06-27
ATE381619T1 (de) 2008-01-15
ZA200108951B (en) 2002-10-30

Similar Documents

Publication Publication Date Title
DK0668292T3 (da) Fremgangsmåde til udvinding af insulin med korrekt forbundne
EP0722461A4 (de) Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird
DE69939991D1 (de) Verfahren zur Gewinnung von heterologen Polypeptiden aus Bakterienzellen
KR19990023651A (ko) 정확하게 결합된 시스틴 브릿지를 갖는 인슐린 전구체를 수득하는 방법
EP1392717A4 (de) Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
DE60311731D1 (de) Verfahren zur rekombinanten Herstellung von Antifusogenikpeptide
DE60021318D1 (de) Verfahren zur Herstellung von natürlich gefalteten Proteinen durch Co-Sekretion von molekularen Chaperonen
DE60037508D1 (de) Verfahren zur herstellung von rekombinantem insulin durch fusionsproteine
DE3586773D1 (de) Verfahren zur beseitigung von n-endaminosaeureresten aus eukaryotpolypeptidanlagen und so erzeugte polypeptide.
KR900700500A (ko) 재조합 유전자 기술에 의한 에라스타제 억제 폴리펩티드 제조방법 및 에라스타제 억제 폴리펩티드
DK1364030T3 (da) Supersecernerbare peptider, processer til frembringelse herf og parallel forbedring af den secernerede form at et eller flere andre polypeptider
PT79013A (de) Verfahren zur herstellung von insulin-derivaten
NO930428D0 (no) Proteinstrukturen til plantetoksingelonin
CA2324513A1 (en) Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production
ATE253639T1 (de) Verfahren zur herstellung von peptiden
PT704527E (pt) Sequencias de adn que codificam precursores de insulina biossinteticos e processo para a preparacao de insulina
ATE46188T1 (de) Positionsspezifische proteolyse mittels renin.
ATE414164T1 (de) Verfahren zur kontrolle der spaltung mit ompt protease
DK595187D0 (da) Minactivin og fremgangsmaade til fremstilling deraf
KR900008040A (ko) 스트렙토마이세테스 내에서 인슐린 전구체를 제조하는 방법
EA200501508A1 (ru) Семейство секретируемых белков
BR9807616A (pt) Aminopeptidase derivada de bacillus licheniformis e processo para preparação de proteìnas tipo natural
DE69132845D1 (de) Verfahren zur Herstellung von Proteinen durch Verwendung von CKS-Fusionsproteinen
ATE139538T1 (de) Verfahren zur selektiven spaltung von fusionsproteinen
ATE547427T1 (de) Verfahren zur rekombinanten herstellung von ribonukleoproteinen

Legal Events

Date Code Title Description
8381 Inventor (new situation)

Inventor name: OKA, SHUSAKU, KITASOMA-GUN, IBARAKI 302-0104, JP

Inventor name: SATO, SEIJI, KITASOMA-GUN, IBARAKI 302-0104, JP

Inventor name: HIGASHIKUNI, NAOHIKO, KITASOMA-GUN, IBARAKI 30, JP

Inventor name: KONDO, MASAAKI, KITASOMA-GUN, IBARAKI 302-0104, JP

Inventor name: KUDO, TOSHIYUKI, KITASOMA-GUN, IBARAKI 302-010, JP

Inventor name: WATANABE, SHIGEAKI, KITASOMA-GUN, IBARAKI 302-, JP

Inventor name: WAKI, YOSHIHIRO, KITASOMA-GUN, IBARAKI 302-010, JP

Inventor name: YUKI, HIROTAKA, KITASOMA-GUN, IBARAKI 302-0104, JP

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee