DE60036633D1 - Mutantenluciferase - Google Patents

Mutantenluciferase

Info

Publication number
DE60036633D1
DE60036633D1 DE60036633T DE60036633T DE60036633D1 DE 60036633 D1 DE60036633 D1 DE 60036633D1 DE 60036633 T DE60036633 T DE 60036633T DE 60036633 T DE60036633 T DE 60036633T DE 60036633 D1 DE60036633 D1 DE 60036633D1
Authority
DE
Germany
Prior art keywords
luciferase
amino acid
compared
wild
type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60036633T
Other languages
English (en)
Other versions
DE60036633T2 (de
Inventor
David James Squirrell
Melenie Jane Murphy
Rachel Louise Price
Peter John White
Tara Louise Depar Willey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9925161.3A external-priority patent/GB9925161D0/en
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of DE60036633D1 publication Critical patent/DE60036633D1/de
Application granted granted Critical
Publication of DE60036633T2 publication Critical patent/DE60036633T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE60036633T 1999-10-26 2000-10-26 Mutierte Luciferase Expired - Lifetime DE60036633T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9925161.3A GB9925161D0 (en) 1999-10-26 1999-10-26 Novel enzyme
GB9925161 1999-10-26
GBGB0016744.5A GB0016744D0 (en) 1999-10-26 2000-07-10 Novel enzyme
GB0016744 2000-07-10
PCT/GB2000/004133 WO2001031028A2 (en) 1999-10-26 2000-10-26 Mutant luciferase

Publications (2)

Publication Number Publication Date
DE60036633D1 true DE60036633D1 (de) 2007-11-15
DE60036633T2 DE60036633T2 (de) 2008-07-03

Family

ID=26244619

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60036633T Expired - Lifetime DE60036633T2 (de) 1999-10-26 2000-10-26 Mutierte Luciferase

Country Status (12)

Country Link
EP (1) EP1224294B1 (de)
JP (1) JP5154724B2 (de)
CN (1) CN100516223C (de)
AT (1) ATE374820T1 (de)
AU (1) AU772485C (de)
CA (1) CA2387691A1 (de)
DE (1) DE60036633T2 (de)
DK (1) DK1224294T3 (de)
ES (1) ES2292479T3 (de)
NZ (1) NZ518413A (de)
RU (1) RU2251571C2 (de)
WO (1) WO2001031028A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) * 1998-10-28 1998-12-23 Secr Defence Novel enzyme
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
GB2400659B8 (en) 2003-04-17 2007-07-09 Cambrex Bio Science Nottingham Assay methods and materials
EP1687399A4 (de) * 2003-10-03 2007-09-05 Univ Michigan Tech Modifizierte luciferase
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
US8147842B2 (en) 2006-06-12 2012-04-03 National Institute Of Advanced Industrial Science And Technology Mutant luciferase
JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
JP5753083B2 (ja) 2008-07-22 2015-07-22 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ Ａｄｐ検出に基づく発光ホスホトランスフェラーゼまたはａｔｐヒドロラーゼのアッセイ法
JP2011120559A (ja) * 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
PL3181687T3 (pl) 2009-05-01 2018-11-30 Promega Corporation Syntetyczne lucyferazy z krewetki oplophorus emitującej bardziej intensywne światło
US8980603B2 (en) 2009-06-30 2015-03-17 The General Hospital Corporation Gaussia luciferase variant for high-throughput screening
BR112013010484B1 (pt) 2010-11-02 2019-09-03 Promega Corp derivados de coelenterazina e métodos de utilizar os mesmos
DK2635582T3 (da) * 2010-11-02 2016-03-14 Promega Corp Hidtil ukendte coelenterazinsubstrater og fremgangsmåder til anvendelse deraf
JP5896679B2 (ja) 2011-03-15 2016-03-30 オリンパス株式会社 オオオバボタル由来ルシフェラーゼ
RU2495929C1 (ru) * 2012-05-30 2013-10-20 ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ УЧРЕЖДЕНИЕ НАУКИ Институт биофизики Сибирского отделения Российской академии наук УКОРОЧЕННАЯ МУТАНТНАЯ ЛЮЦИФЕРАЗА ИЗ Metridia longa ДЛЯ ПРИМЕНЕНИЯ В КАЧЕСТВЕ БИОЛЮМИНЕСЦЕНТНОГО РЕПОРТЕРА В ЖИВЫХ КЛЕТКАХ
EP3099691B1 (de) 2014-01-29 2019-11-20 Promega Corporation Pro-substrate für lebendzellanwendungen
EP3099683B1 (de) 2014-01-29 2020-08-05 Promega Corporation Chinonmaskierte sonden als markierungsreagenzien für zellaufnahmemessungen
JP6811173B2 (ja) 2014-09-11 2021-01-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP6510041B2 (ja) 2014-10-08 2019-05-08 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生物発光サクシネート検出アッセイ
RU2674894C2 (ru) 2017-01-30 2018-12-13 Общество с ограниченной ответственностью "ПЛАНТА" Новые люциферазы и способы их использования
GB201805396D0 (en) * 2018-04-01 2018-05-16 Univ College Cardiff Consultants Ltd Modified Luciferases
CN110456052A (zh) * 2019-08-22 2019-11-15 中国科学院武汉病毒研究所 一种血清中***抗体的快速检测方法及其应用
EP4105336A4 (de) 2020-02-14 2024-04-10 Kikkoman Corporation Flüssige zusammensetzung zur messung von atp sowie amp und/oder adp in proben
CN114015664B (zh) * 2021-12-06 2023-07-21 郑州伊美诺生物技术有限公司 荧光素酶突变体及其应用
CN114350627B (zh) * 2021-12-16 2022-08-05 大连博格林生物科技有限公司 一种萤火虫荧光素酶突变体及其制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3048466B2 (ja) * 1991-06-27 2000-06-05 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
ES2301160T3 (es) * 1994-01-03 2008-06-16 Promega Corporation Luciferasas mutantes.
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases
CA2210354C (en) * 1995-01-20 2008-01-08 The Secretary Of State For Defence Mutant luciferases
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
AU754312C (en) * 1997-09-19 2005-11-10 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) * 1998-10-28 1998-12-23 Secr Defence Novel enzyme

Also Published As

Publication number Publication date
ES2292479T3 (es) 2008-03-16
AU1042501A (en) 2001-05-08
CA2387691A1 (en) 2001-05-03
AU772485B2 (en) 2004-04-29
JP5154724B2 (ja) 2013-02-27
CN100516223C (zh) 2009-07-22
WO2001031028A2 (en) 2001-05-03
RU2002113653A (ru) 2004-01-27
RU2251571C2 (ru) 2005-05-10
JP2003512071A (ja) 2003-04-02
WO2001031028A3 (en) 2002-05-02
EP1224294B1 (de) 2007-10-03
DE60036633T2 (de) 2008-07-03
CN1413252A (zh) 2003-04-23
EP1224294A2 (de) 2002-07-24
DK1224294T3 (da) 2008-02-04
AU772485C (en) 2004-11-18
NZ518413A (en) 2004-11-26
ATE374820T1 (de) 2007-10-15

Similar Documents

Publication Publication Date Title
DK1224294T3 (da) Mutant luciferase
DE69940208D1 (de) Mutierte luciferase mit verbesserter thermostabilität
DK0973874T3 (da) Enzymassay for mutant ildflue-luciferase
DE69535744D1 (de) Mutierte luziferasen
ATE311461T1 (de) Thermostabile luciferase aus photuris pennsylvanica und pyrophorus plagiophthalamus und hertsellungsmethoden
Law et al. Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance
KR970702369A (ko) 루시퍼라제(Luciferases)
EP2284271A3 (de) Mutierte Luziferase
CA2105984A1 (en) Cloning and expression of renilla luciferase
JP2003512071A5 (de)
ATE376585T1 (de) Phenol oxydierende enzyme und deren verwendung
ATE472554T1 (de) Aminosäureaustauschmutanten von interleukin 13
JP2004516828A (ja) 単離されたルシフェラーゼおよびその使用
JP2008289475A (ja) 最大発光波長がシフトした変異型ルシフェラーゼ
US7807429B2 (en) Isolated luciferase gene of L. italica
Wienhausen et al. Luciferases from different species of fireflies are antigenically similar
TR200002713T2 (tr) Fenol oksitleyici enzimler ve kullanımları
WO2003044223A3 (en) Phosphine-containing formulations for chemiluminescent luciferase assays
Puri et al. Purification and characterization of dexamethasone inducible hepatic cytochrome P450 isozymes from rhesus monkey
FR3092587A1 (fr) Proteines bioluminescentes aux proprietes ameliorees
Squirrell et al. Enzyme assay for mutant firefly luciferase
MXPA96004194A (en) Lucifera

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: 3M INNOVATIVE PROPERTIES CO., ST. PAUL, MINN., US

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: PROMEGA CORP., MADISON, WIS., US