DE10342794A1 - Sekretion von Proteinen aus Hefen - Google Patents
Sekretion von Proteinen aus Hefen Download PDFInfo
- Publication number
- DE10342794A1 DE10342794A1 DE10342794A DE10342794A DE10342794A1 DE 10342794 A1 DE10342794 A1 DE 10342794A1 DE 10342794 A DE10342794 A DE 10342794A DE 10342794 A DE10342794 A DE 10342794A DE 10342794 A1 DE10342794 A1 DE 10342794A1
- Authority
- DE
- Germany
- Prior art keywords
- shuttle
- nucleic acid
- proteins
- acid sequences
- peptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Abstract
Die vorliegende Erfindung betrifft Expressionskonstrukte, umfassend die kodierende Nukleinsäuresequenz für ein von Hefezellen prozessierbares Shuttlepeptidkonstrukt; entsprechende Expressionsvektoren, enthaltend derartige Konstrukte; mit deren Hilfe durchgeführte Verfahren zur rekombinanten Herstellung von Zielproteinen; damit transformierte Wirte; Shuttlepeptide und dafür kodierende Nukleinsäuresequenzen; Nukleinsäuresequenzen, kodierend für solche Shuttlepeptide, fusioniert mit einem Fremdprotein; Hydrophobin-Proteine, welche unter Verwendung von derartigen Shuttlepeptiden hergestellt wurden, sowie die Verwendung Hydrophobinen zur Beschichtung von Gegenständen, wie z. B. Leder.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10342794A DE10342794A1 (de) | 2003-09-16 | 2003-09-16 | Sekretion von Proteinen aus Hefen |
CNA2004800265223A CN1852983A (zh) | 2003-09-16 | 2004-09-15 | 蛋白质从酵母的分泌 |
EP04765253A EP1664306A2 (de) | 2003-09-16 | 2004-09-15 | Sekretion von proteinen aus hefen |
JP2006525798A JP2007505602A (ja) | 2003-09-16 | 2004-09-15 | 酵母からのタンパク質の分泌 |
CA002537492A CA2537492A1 (en) | 2003-09-16 | 2004-09-15 | Secretion of proteins from yeasts |
US10/572,189 US20070077619A1 (en) | 2003-09-15 | 2004-09-15 | Secretion of proteins from yeasts |
PCT/EP2004/010346 WO2005033316A2 (de) | 2003-09-16 | 2004-09-15 | Sekretion von proteinen aus hefen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10342794A DE10342794A1 (de) | 2003-09-16 | 2003-09-16 | Sekretion von Proteinen aus Hefen |
Publications (1)
Publication Number | Publication Date |
---|---|
DE10342794A1 true DE10342794A1 (de) | 2005-04-21 |
Family
ID=34352870
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE10342794A Withdrawn DE10342794A1 (de) | 2003-09-15 | 2003-09-16 | Sekretion von Proteinen aus Hefen |
Country Status (7)
Country | Link |
---|---|
US (1) | US20070077619A1 (de) |
EP (1) | EP1664306A2 (de) |
JP (1) | JP2007505602A (de) |
CN (1) | CN1852983A (de) |
CA (1) | CA2537492A1 (de) |
DE (1) | DE10342794A1 (de) |
WO (1) | WO2005033316A2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006131478A2 (de) * | 2005-06-06 | 2006-12-14 | Basf Aktiengesellschaft | Verfahren zur beschichtung von oberflächen von faserigen substraten |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006082251A2 (de) | 2005-02-07 | 2006-08-10 | Basf Aktiengesellschaft | Neue hydrophobinfusionsproteine, deren herstellung und verwendung |
TW200639179A (en) * | 2005-02-07 | 2006-11-16 | Basf Ag | Method for coating surfaces with hydrophobins |
JP4964227B2 (ja) * | 2005-03-30 | 2012-06-27 | ビーエーエスエフ ソシエタス・ヨーロピア | 硬化無機物建材、自然石、人造石、及びセラミックの表面処理用のハイドロフォビンの使用方法 |
EP1866401B1 (de) * | 2005-03-30 | 2010-09-29 | Basf Se | Verwendung von hydrophobinen zur schmutzabweisenden behandlung von harten oberflächen |
KR20080004555A (ko) | 2005-03-31 | 2008-01-09 | 바스프 악티엔게젤샤프트 | 접착 촉진제로서의 폴리펩티드의 용도 |
JP2008534554A (ja) | 2005-04-01 | 2008-08-28 | ビーエーエスエフ ソシエタス・ヨーロピア | 乳化破壊剤としての蛋白質の使用 |
US7799741B2 (en) | 2005-04-01 | 2010-09-21 | Basf Se | Drilling mud containing hydrophobin |
DE102005027039A1 (de) * | 2005-06-10 | 2006-12-21 | Basf Ag | Hydrophobin als Beschichtungsmittel für expandierbare oder expandierte, thermoplastische Polymerpartikel |
DE102005027139A1 (de) | 2005-06-10 | 2006-12-28 | Basf Ag | Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung |
DE102005029704A1 (de) * | 2005-06-24 | 2007-01-11 | Basf Ag | Verwendung von Hydrophobin-Polypeptiden sowie Konjugaten aus Hydrophobin-Polypeptiden mit Wirk-oder Effektstoffen und ihre Herstellung sowie deren Einsatz in der Kosmetik |
EP1913143A1 (de) * | 2005-07-29 | 2008-04-23 | Pharmexa A/S | Verbesserte proteinexpression |
KR20080041228A (ko) * | 2005-08-01 | 2008-05-09 | 바스프 에스이 | 표면활성 비효소 단백질의 직물을 세척하기 위한 용도 |
DE102005048720A1 (de) | 2005-10-12 | 2007-04-19 | Basf Ag | Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen |
CN101501432B (zh) | 2006-08-15 | 2011-10-12 | 巴斯夫欧洲公司 | 制备干燥的自由流动的疏水蛋白制剂的方法 |
US8455107B2 (en) * | 2007-03-12 | 2013-06-04 | Basf Se | Method of treating cellulosic materials with hydrophobins |
US20110126143A1 (en) * | 2009-11-24 | 2011-05-26 | Williams Michael D | System and method for interfacing fitness equipment and physiological monitoring |
WO2012004255A1 (de) * | 2010-07-07 | 2012-01-12 | Basf Se | Zusammensetzung enthaltend ein hydrophobin und verfahren zum reinigen von hydrophoben oberflächen |
EP2631296A1 (de) | 2012-02-22 | 2013-08-28 | Kimmo Koivu | Verfahren zur Hydrophobinherstellung in Pflanzen und Verfahren zur Herstellung von Hydrophobinmultimeren in Pflanzen und Mikroben |
CA3129665A1 (en) | 2019-03-21 | 2020-09-24 | Onxeo | A dbait molecule in combination with kinase inhibitor for the treatment of cancer |
WO2021089791A1 (en) | 2019-11-08 | 2021-05-14 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for the treatment of cancers that have acquired resistance to kinase inhibitors |
CN114761418A (zh) * | 2019-12-02 | 2022-07-15 | 株式会社Lg化学 | Cho细胞衍生的蛋白分泌因子和包含它的表达载体 |
WO2021148581A1 (en) | 2020-01-22 | 2021-07-29 | Onxeo | Novel dbait molecule and its use |
KR102466926B1 (ko) * | 2020-05-20 | 2022-11-14 | 전남대학교산학협력단 | 하이드로포빈의 가용성 발현 방법 및 정제 방법 |
CN115701464A (zh) * | 2021-08-02 | 2023-02-10 | 江苏金太阳纺织科技股份有限公司 | 超疏水整理剂及其制备方法与应用 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1196484B (it) * | 1986-07-11 | 1988-11-16 | Sclavo Spa | Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe |
US5010182A (en) * | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
JP3508157B2 (ja) * | 1993-05-19 | 2004-03-22 | 旭硝子株式会社 | ***酵母接合フェロモン前駆体遺伝子 |
GB2281421B (en) * | 1993-08-23 | 1998-04-01 | Advanced Risc Mach Ltd | Integrated circuit |
EP0662515B1 (de) * | 1993-12-10 | 2000-11-15 | Korea Institute Of Science And Technology | Signalsequenzen für die Secretion heterologer Proteine von Hefe |
JP3730256B2 (ja) * | 1995-02-03 | 2005-12-21 | 旭硝子株式会社 | 分泌シグナル遺伝子およびそれを有する発現ベクター |
AU5914196A (en) * | 1995-06-12 | 1997-01-09 | Proefstation Voor De Champignoncultuur | Hydrophobins from edible fungi, genes, nucleotide sequences and dna-fragments encoding for said hydrophobins, and expression thereof |
AU5465498A (en) * | 1996-12-13 | 1998-07-03 | Michael Kunitani | Analysis and separation of platelet-derived growth factor proteins |
WO1999023223A1 (en) * | 1997-10-31 | 1999-05-14 | Asahi Glass Company Ltd. | Induction promoter gene and secretory signal gene usable in $i(schizosaccharomyces pombe), expression vectors having the same, and use thereof |
AU2001292574A1 (en) * | 2000-09-06 | 2002-03-22 | Zymogenetics Inc. | Human phermone polypeptide |
GB0030038D0 (en) * | 2000-12-08 | 2001-01-24 | Univ Warwick | Yeast-based assay |
FR2833490B1 (fr) * | 2001-12-14 | 2004-12-10 | Oreal | Utilisition cosmetique d'au moins une hydrophobine pour le traitement des matieres keratiniques et compositions mises en oeuvre |
-
2003
- 2003-09-16 DE DE10342794A patent/DE10342794A1/de not_active Withdrawn
-
2004
- 2004-09-15 CN CNA2004800265223A patent/CN1852983A/zh active Pending
- 2004-09-15 JP JP2006525798A patent/JP2007505602A/ja not_active Withdrawn
- 2004-09-15 CA CA002537492A patent/CA2537492A1/en not_active Abandoned
- 2004-09-15 EP EP04765253A patent/EP1664306A2/de not_active Withdrawn
- 2004-09-15 US US10/572,189 patent/US20070077619A1/en not_active Abandoned
- 2004-09-15 WO PCT/EP2004/010346 patent/WO2005033316A2/de not_active Application Discontinuation
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006131478A2 (de) * | 2005-06-06 | 2006-12-14 | Basf Aktiengesellschaft | Verfahren zur beschichtung von oberflächen von faserigen substraten |
WO2006131478A3 (de) * | 2005-06-06 | 2007-03-22 | Basf Ag | Verfahren zur beschichtung von oberflächen von faserigen substraten |
Also Published As
Publication number | Publication date |
---|---|
US20070077619A1 (en) | 2007-04-05 |
WO2005033316A3 (de) | 2005-10-06 |
EP1664306A2 (de) | 2006-06-07 |
CN1852983A (zh) | 2006-10-25 |
JP2007505602A (ja) | 2007-03-15 |
CA2537492A1 (en) | 2005-04-14 |
WO2005033316A2 (de) | 2005-04-14 |
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Legal Events
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8130 | Withdrawal |