DE02780078T1 - Ektokornea-ähnliches blatt und herstellungsverfahren dafür - Google Patents

Ektokornea-ähnliches blatt und herstellungsverfahren dafür Download PDF

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Publication number
DE02780078T1
DE02780078T1 DE2002780078 DE02780078T DE02780078T1 DE 02780078 T1 DE02780078 T1 DE 02780078T1 DE 2002780078 DE2002780078 DE 2002780078 DE 02780078 T DE02780078 T DE 02780078T DE 02780078 T1 DE02780078 T1 DE 02780078T1
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layer
cells
epithelial
corneal
oral mucosal
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DE2780078T9 (de
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Takahiro Nakamura
Shigeru Kinoshita
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Amniotec Inc
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Amniotec Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/14—Eye parts, e.g. lenses, corneal implants; Implanting instruments specially adapted therefor; Artificial eyes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3666—Epithelial tissues other than skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Vascular Medicine (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Tents Or Canopies (AREA)
  • External Artificial Organs (AREA)
  • Adornments (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Eine korneale, Epithel-ähnliche Schicht, umfassend eine Zellschicht, in welcher eine geschichtete Struktur von Zellen ausgebildet ist, die von Mundschleimhaut-Epithelzellen abgeleitet sind.

Claims (10)

  1. Eine korneale, Epithel-ähnliche Schicht, umfassend eine Zellschicht, in welcher eine geschichtete Struktur von Zellen ausgebildet ist, die von Mundschleimhaut-Epithelzellen abgeleitet sind.
  2. Die korneale, Epithel-ähnliche Schicht nach Anspruch 1, wobei die Zellen der äußersten Schicht nicht verhornt sind und/oder flach geformt sind.
  3. Die korneale, Epithel-ähnliche Schicht nach Anspruch 1 oder 2, wobei die korneale, Epithel-ähnliche Schicht eine Barrierefunktion hat.
  4. Eine korneale Epithel-Transplantations-Schicht, umfassend eine Zellschicht, wie in Anspruch 1 oder 2 definiert, in welcher eine geschichtete Struktur von Zellen auf einer Kollagen-Schicht ausgebildet ist.
  5. Die korneale Epithel-Transplantations-Schicht nach Anspruch 4, wobei die Schicht eine Barrierefunktion hat.
  6. Ein Verfahren zur Herstellung einer kornealen, Epithel-ähnlichen Schicht, das Verfahren umfassend: a) Kultivierung von Mundschleimhaut-Epithelzellen auf einer Kollagen-Schicht und b) nachdem die Mundschleimhaut-Epithelzellen proliferiert sind und eine geschichtete Struktur der Zellen ausgebildet ist, das In-Kontakt-Bringen der äußersten Schicht mit Luft.
  7. Das Verfahren nach Anspruch 6, wobei Schritt a) unter der Koexistenz von Trägerzellen durchgeführt wird.
  8. Das Verfahren nach Anspruch 6 oder 7, wobei Schritt a) unter Koexistenz von Trägerzellen und in einem Stadium durchgeführt wird, in welchem zwischen den Trägerzellen und der Kollagen-Schicht eine isolierende Membran mit einer Porengröße existiert, durch welche die Trägerzellen nicht hindurch passieren können.
  9. Ein Verfahren zur Herstellung einer kornealen, Epithel-ähnlichen Schicht, umfassend die Schritte: (i) Inokulation von Trägerzellen in einen ersten Behälter zur Ausbildung einer Trägerzellschicht; (ii) Platzierung eines zweiten Behälters, welcher einen Behälterboden aufweist, der aus einer isolierenden Membran mit einer Porengröße besteht, durch welche die Trägerzellen nicht hindurch passieren können, in den ersten Behälter, so dass sich der Behälterboden in Kulturmedium befindet; (iii) Ausbildung einer Kollagen-Schicht auf dem Behälterboden des zweiten Behälters; (iv) Inokulation von Mundschleimhaut-Epithelzellen auf der Kollagen-Schicht; (v) Kultivierung der Mundschleimhaut-Epithelzellen zur Ausbildung einer geschichteten Struktur der Zellen, und (vi) das In-Kontakt-Bringen der äußersten Schicht der geschichteten Struktur der Mundschleimhaut-Epithelzellen mit Luft.
  10. Die korneale Epithel-Transplantations-Schicht von Anspruch 4 oder 5 oder das Verfahren nach einem der Ansprüche 7 bis 9, wobei die Kollagen-Schicht abgeleitet wird von der Amnion-Membran und/oder aus einer Amnion-Membran besteht, aus welcher das Epithel entfernt wurde.
DE02780078T 2001-11-19 2002-11-13 Ektokornea-ähnliches blatt und herstellungsverfahren dafür Active DE2780078T9 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2001352675 2001-11-19
JP2001352675 2001-11-19
PCT/JP2002/011857 WO2003043542A1 (en) 2001-11-19 2002-11-13 Ectocornea-like sheet and method of constructing the same

Publications (2)

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DE02780078T1 true DE02780078T1 (de) 2005-01-13
DE2780078T9 DE2780078T9 (de) 2005-05-19

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US (3) US20050003532A1 (de)
EP (1) EP1454600B1 (de)
JP (1) JP4392855B2 (de)
KR (1) KR100940765B1 (de)
CN (1) CN100360095C (de)
AT (1) ATE522185T1 (de)
AU (1) AU2002343774A1 (de)
CA (1) CA2466903A1 (de)
DE (1) DE2780078T9 (de)
ES (1) ES2372814T3 (de)
WO (1) WO2003043542A1 (de)

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JP4576545B2 (ja) 2004-05-27 2010-11-10 独立行政法人理化学研究所 羊膜由来因子による胚性幹細胞の培養方法
US20080026030A1 (en) * 2004-06-30 2008-01-31 Arblast Co., Ltd. Corneal Epithelial Sheet and Process for Producing the Same
WO2006129673A1 (ja) * 2005-05-30 2006-12-07 Arblast Co., Ltd. 組織再建用材料及びその使用
EP1944045B1 (de) * 2005-07-25 2013-09-18 Foundation for Biomedical Research and Innovation Flächenartige zusammensetzung
EP1932549B1 (de) * 2005-08-26 2016-12-28 AMNOS Co., Ltd. Getrocknetes amnion und verfahren zur trocknung von amnion
WO2007029676A1 (ja) * 2005-09-07 2007-03-15 Arblast Co., Ltd. 生体組織シート及びその作製方法
CN1916166B (zh) * 2006-09-08 2010-08-11 北京赛尔泰和生物医药科技有限公司 自体角膜上皮的制备方法
JP5181172B2 (ja) * 2007-02-21 2013-04-10 国立大学法人富山大学 培養重層上皮シートの作製方法
KR100996846B1 (ko) 2008-03-03 2010-11-26 주식회사 엠씨티티 비점막 상피세포를 이용한 각막 또는 결막 조직 시트
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JP5475640B2 (ja) 2008-03-19 2014-04-16 千寿製薬株式会社 ラクリチンの部分ペプチド
CN102725399B (zh) * 2009-12-18 2015-03-11 上海赛傲生物技术有限公司 制备多能干细胞的材料和方法
DE102010001271B8 (de) * 2010-01-27 2012-12-06 Urotiss Gmbh Vernähbares Gewebetransplantatkonstrukt zur Rekonstruktion eines menschlichen oder tierischen Organs
CN101757690B (zh) * 2010-02-05 2013-04-24 陕西瑞盛生物科技有限公司 一种制备组织工程角膜的方法
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US8883210B1 (en) 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
WO2016114242A1 (ja) 2015-01-13 2016-07-21 国立大学法人大阪大学 表面外胚葉系細胞から誘導された角膜上皮様細胞
EP3297694A1 (de) 2015-05-21 2018-03-28 Musculoskeletal Transplant Foundation Modifizierte demineralisierte kortikale knochenfasern
CN105238691A (zh) * 2015-11-13 2016-01-13 张志华 一种用于人工角膜重建的细胞种植模型
KR20170002194U (ko) 2016-03-29 2017-06-21 다나제약 주식회사 분할형 의료 패치
CN109312301B (zh) * 2016-04-20 2023-06-16 京都府公立大学法人 用于制备培养上皮细胞片的方法
CN108567995A (zh) * 2017-03-10 2018-09-25 上海睿泰生物科技股份有限公司 一种用于角膜疾病治疗的上皮细胞片的制备方法及其应用
CN107335096A (zh) * 2017-07-11 2017-11-10 上海白衣缘生物工程有限公司 一种复合口腔粘膜上皮细胞的口腔补片制备方法
CN113143584B (zh) * 2021-03-09 2023-07-28 河南省立眼科医院 动物角膜上皮细胞收集及低温保存手持设备、收集方法

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Publication number Publication date
EP1454600B1 (de) 2011-08-31
KR20040072635A (ko) 2004-08-18
AU2002343774A1 (en) 2003-06-10
KR100940765B1 (ko) 2010-02-10
CA2466903A1 (en) 2003-05-30
JPWO2003043542A1 (ja) 2005-03-10
US20050003532A1 (en) 2005-01-06
JP4392855B2 (ja) 2010-01-06
WO2003043542A1 (en) 2003-05-30
ES2372814T3 (es) 2012-01-26
CN1589126A (zh) 2005-03-02
EP1454600A4 (de) 2010-04-07
EP1454600A1 (de) 2004-09-08
ATE522185T1 (de) 2011-09-15
DE2780078T9 (de) 2005-05-19
US20140220102A1 (en) 2014-08-07
CN100360095C (zh) 2008-01-09
US20050287126A1 (en) 2005-12-29

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