CY1112852T1 - Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων - Google Patents

Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων

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Publication number
CY1112852T1
CY1112852T1 CY20121100439T CY121100439T CY1112852T1 CY 1112852 T1 CY1112852 T1 CY 1112852T1 CY 20121100439 T CY20121100439 T CY 20121100439T CY 121100439 T CY121100439 T CY 121100439T CY 1112852 T1 CY1112852 T1 CY 1112852T1
Authority
CY
Cyprus
Prior art keywords
dna
remote sample
methods
compositions
dynahylation
Prior art date
Application number
CY20121100439T
Other languages
English (en)
Inventor
Matthias Ballhause
Kurt Berlin
Theo Devos
Dimo Dietrich
Volker Liebenberg
Cathy Lofton-Day
Joe Lograsso
Jennifer Maas
Fabian Model
Matthias Schuster
Andrew Sledziewski
Reimo Tetzner
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Publication of CY1112852T1 publication Critical patent/CY1112852T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • General Chemical & Material Sciences (AREA)

Abstract

Εκδοχές της παρούσας εφεύρεσης σχετίζονται με συνθέσεις και μεθόδους αποκόμισης θραυσμάτων DΝΑ από ένα εξωτερικό δείγμα. Σε συγκεκριμένες εκδοχές αποκομίζεται ένα εξωτερικό δείγμα που περιέχει DΝΑ, το DΝΑ απομονώνεται από το εξωτερικό δείγμα και το απομονωμένο DΝΑ κατεργάζεται κατά τρόπο που επιτρέπει διαφοροποίηση της μεθυλιωμένης από τη μη μεθυλιωμένη κυτοσίνη. Επιπρόσθετες, συγκεκριμένες υλοποιήσεις παρέχουν συνθέσεις και μεθόδους ανάλυσης της μεθυλίωσης του DΝΑ ενός εξωτερικού δείγματος. Άλλες εκδοχές παρέχουν συνθέσεις και μεθόδους ενίσχυσης όλου του γενώματος DΝΑ κατεργασμένου με όξινο θειώδες.
CY20121100439T 2005-04-15 2012-05-11 Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων CY1112852T1 (el)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US67224205P 2005-04-15 2005-04-15
US67699705P 2005-05-02 2005-05-02
US69752105P 2005-07-08 2005-07-08
US72360205P 2005-10-04 2005-10-04
US78024806P 2006-03-08 2006-03-08
EP06750657A EP1871912B1 (en) 2005-04-15 2006-04-17 Method for determining DNA methylation in blood or urine samples

Publications (1)

Publication Number Publication Date
CY1112852T1 true CY1112852T1 (el) 2016-02-10

Family

ID=36808881

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20121100439T CY1112852T1 (el) 2005-04-15 2012-05-11 Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων

Country Status (13)

Country Link
US (1) US10731215B2 (el)
EP (2) EP1871912B1 (el)
JP (2) JP5133238B2 (el)
AT (1) ATE547537T1 (el)
AU (1) AU2006236363B2 (el)
CA (1) CA2603815C (el)
CY (1) CY1112852T1 (el)
DK (1) DK1871912T3 (el)
ES (1) ES2382746T3 (el)
PL (1) PL1871912T3 (el)
PT (1) PT1871912E (el)
SI (1) SI1871912T1 (el)
WO (1) WO2006113770A1 (el)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1863927B (zh) 2003-09-04 2010-08-11 人类遗传标记控股有限公司 核酸检测分析
NZ555620A (en) 2004-12-03 2008-08-29 Human Genetic Signatures Pty Methods for simplifying microbial nucleic acids by chemical modification of cytosines
CA2621932A1 (en) 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for hpv and methylated genomic dna targets to screen for cervical cancer
GB0700374D0 (en) 2007-01-09 2007-02-14 Oncomethylome Sciences S A NDRG family methylation markers
WO2008135512A2 (en) * 2007-05-02 2008-11-13 Jerzy Paszkowski Dna amplification method
DK2479289T3 (en) 2007-06-08 2016-05-02 Epigenomics Ag Method for methylation analysis
US8685675B2 (en) 2007-11-27 2014-04-01 Human Genetic Signatures Pty. Ltd. Enzymes for amplification and copying bisulphite modified nucleic acids
EP2071035A1 (en) 2007-12-13 2009-06-17 Epigenomics AG Facilitator and method for amplification
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
EP2362910A4 (en) * 2008-11-03 2012-05-09 Alchemy Biosciences Pty Ltd METHOD FOR DNA CLONING
EP2189538A1 (en) 2008-11-21 2010-05-26 Epigenomics AG An analysis molecule and method for the analysis of a nucleotide position in a nucleic acid
US9234456B2 (en) 2009-10-06 2016-01-12 Cummins Ltd. Turbomachine
WO2011135058A2 (en) 2010-04-30 2011-11-03 Mdxhealth Sa Methods for detecting epigenetic modifications
GB201105726D0 (en) 2011-04-04 2011-05-18 Cummins Ltd A turbine
US10140420B2 (en) * 2011-10-12 2018-11-27 Merge Healthcare Incorporation Systems and methods for independent assessment of image data
ES2669214T3 (es) 2011-12-13 2018-05-24 Oslo Universitetssykehus Hf Procedimientos y kits para la detección de estado de metilación
CN104955960A (zh) 2012-11-30 2015-09-30 剑桥表现遗传学有限公司 用于修饰的核苷酸的氧化剂
US10203346B2 (en) * 2013-10-22 2019-02-12 Hitachi High-Technologies Corporation Automatic analysis device
US11459573B2 (en) 2015-09-30 2022-10-04 Trustees Of Boston University Deadman and passcode microbial kill switches
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
KR102436270B1 (ko) 2016-05-05 2022-08-25 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 폐 종양의 검출
WO2018017710A1 (en) 2016-07-19 2018-01-25 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
WO2018140781A1 (en) 2017-01-27 2018-08-02 Exact Sciences Development Company, Llc Detection of colon neoplasia by analysis of methylated dna
JP7220200B2 (ja) 2017-04-19 2023-02-09 シングレラ ジェノミクス， インコーポレイテッド ライブラリー構築および配列解析のための組成物および方法
CN107058553A (zh) * 2017-05-05 2017-08-18 武汉爱基百客生物科技有限公司 一种分子ssr标记技术
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CA3092998A1 (en) 2018-03-13 2019-09-19 Grail, Inc. Anomalous fragment detection and classification
EP4034676A1 (en) 2020-07-30 2022-08-03 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
CN112708546B (zh) * 2021-01-13 2023-11-07 苏州缔因安生物科技有限公司 一种全集成核酸即时检测装置及其应用
US11332777B1 (en) * 2021-10-28 2022-05-17 URIT Medical Electronic Co., Ltd. Methods and reagent kits for bisulfite conversion of DNA

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565332A (en) * 1991-09-23 1996-10-15 Medical Research Council Production of chimeric antibodies - a combinatorial approach
US5532127A (en) * 1992-01-27 1996-07-02 Icos Corporation Assay for 1-CAM related protein expression
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
WO1998056952A1 (en) 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
AU3377500A (en) * 1999-02-26 2000-09-14 Myriad Genetics, Inc. Method for extracting dna from dried specimens
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US6709818B1 (en) * 1999-10-13 2004-03-23 The Johns Hopkins University School Of Medicine Methods of diagnosing and treating hepatic cell proliferative disorders
DE19957827C2 (de) 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
DE10010281B4 (de) 2000-02-25 2005-03-10 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10019058A1 (de) 2000-04-06 2001-12-20 Epigenomics Ag Detektion von Variationen des DNA-Methylierungsprofils
DE10032529A1 (de) 2000-06-30 2002-02-07 Epigenomics Ag Diagnose von bedeutenden genetischen Parametern innerhalb des Major Histocompatibility Complex (MHC)
JP2004507213A (ja) 2000-03-15 2004-03-11 エピゲノミクス　アーゲー 細胞周期に関連する疾患の診断
DE20121973U1 (de) 2000-03-15 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit dem Zellzyklus assoziierten Krankheiten
DE20121977U1 (de) 2000-03-15 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit Tumor-Suppressorgenen und Onkogenen assoziierten Krankheiten
DE10013847A1 (de) 2000-03-15 2001-09-27 Epigenomics Ag Oligonukleotide oder PNA-Oligomere und Verfahren zur parallelen Detektion des Methylierungszustandes genomischer DNA
DE20121964U1 (de) 2000-04-06 2004-01-15 Epigenomics Ag Nukleinsäuren für die Diagnose von mit dem Metabolismus assoziierten Krankheiten
DE20121969U1 (de) 2000-04-06 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit DNA Reparatur assoziierten Krankheiten
DE20121974U1 (de) 2000-04-06 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit DNA Replikation assoziierten Krankheiten
AU2001278420A1 (en) 2000-04-06 2001-11-07 Epigenomics Ag Diagnosis of diseases associated with dna repair
DE20121965U1 (de) 2000-04-06 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit DNA Addukten assoziierten Krankheiten
DE20121972U1 (de) 2000-04-06 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit DNA Transkription assoziierten Krankheiten
DE20121968U1 (de) 2000-04-06 2003-12-18 Epigenomics Ag Nukleinsäuren für die Diagnose von mit Apoptose assoziierten Krankheiten
DE20121970U1 (de) 2000-04-06 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit der Genregulation assoziierten Krankheiten
DE20121960U1 (de) 2000-04-06 2004-01-15 Epigenomics Ag Nukleinsäuren für die Diagnose von mit Metastase assoziierten Krankheiten
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
DE20121963U1 (de) 2000-06-30 2003-12-18 Epigenomics Ag Nukleinsäuren für die Diagnose von mit Entwicklungsgenen assoziierten Krankheiten
DE20121971U1 (de) 2000-06-30 2003-12-24 Epigenomics Ag Nukleinsäuren für die Pharmakogenomische Methylierungsanalyse
DE20121967U1 (de) 2000-06-30 2003-12-18 Epigenomics Ag Nukleinsäuren für die Diagnose von Verhaltensstörungen, neurologischen Erkrankungen und Krebs
DE20121961U1 (de) 2000-06-30 2003-12-18 Epigenomics Ag Nukleinsäuren für die Diagnose von mit der Signaltransduktion assoziierten Krankheiten
DE20121966U1 (de) 2000-06-30 2003-11-20 Epigenomics Ag Nukleinsäuren für die Diagnose von mit dem Immunsystem assoziierten Krankheiten
DE20121979U1 (de) 2000-06-30 2003-12-24 Epigenomics Ag Nukleinsäuren für die Diagnose von mit der Zellsignalisierung assoziierten Krankheiten
DE20121975U1 (de) 2000-06-30 2004-01-15 Epigenomics Ag Nukleinsäuren für die Differenzierung von Astrocytomen, Oligoastrocytomen und Oligodendrogliom-Tumorzellen
DE20121978U1 (de) 2000-06-30 2004-01-15 Epigenomics Ag Nukleinsäuren für die Analyse von Astrocytomen
JP2004501666A (ja) 2000-06-30 2004-01-22 エピゲノミクス　アーゲー 薬理ゲノミクスのメチル化状態分析のための方法及び核酸
DE10037769A1 (de) 2000-08-03 2002-02-21 Epigenomics Gmbh Diagnose von mit CD24 assoziierten Krankheiten
WO2002018649A1 (en) 2000-08-25 2002-03-07 Lovelace Respiratory Research Institute Nested methylation-specific polymerase chain reaction cancer detection method
WO2002018632A2 (de) 2000-09-01 2002-03-07 Epigenomics Ag Verfahren zur bestimmung des methylierungsgrades von bestimmten cytosinen in genomischer dna im sequenzkontext 5'-cpg-3'
AU2002224328A1 (en) * 2000-09-20 2002-04-02 Case Western Reserve University Methods and compositions for detecting cancers associated with methylation of hmlh1promoter dna
DE10054972A1 (de) 2000-11-06 2002-06-06 Epigenomics Ag Diagnose von mit humos assoziierten Krankheiten
DE10054974A1 (de) 2000-11-06 2002-06-06 Epigenomics Ag Diagnose von mit Cdk4 assoziierten Krankheiten
DE10061338A1 (de) 2000-12-06 2002-06-20 Epigenomics Ag Diagnose von mit Angiogenese assoziierten Krankheiten
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
EP1410304A2 (en) 2001-03-26 2004-04-21 Epigenomics AG Method for epigenetic feature selection
ATE534731T1 (de) * 2001-05-30 2011-12-15 Stratagene California Zusammensetzungen und verfahren zur zufallsmutagenese von nukleinsäuren
DE10128508A1 (de) 2001-06-14 2003-02-06 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren
DE10130800B4 (de) * 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
DE10139283A1 (de) 2001-08-09 2003-03-13 Epigenomics Ag Verfahren und Nukleinsäuren zur Analyse von Colon-Krebs
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
EP1438416A4 (en) 2001-09-26 2007-09-05 Epigenx Pharmaceutical Inc ASSAYS FOR CHANGES TO THE METHYLATION OF DNA
DE10164501A1 (de) 2001-11-23 2003-06-12 Epigenomics Ag Verfahren und Nukleinsäuren für die Analyse einer Lymphoid-Zellteilungsstörung
WO2003044226A2 (en) 2001-11-23 2003-05-30 Epigenomics Ag Method and nucleic acids for the analysis of a lymphoid cell proliferative disorder
DE10161625A1 (de) 2001-12-14 2003-07-10 Epigenomics Ag Verfahren und Nukleinsäuren für die Analyse einer Lungenzell-Zellteilungsstörung
US20030215842A1 (en) * 2002-01-30 2003-11-20 Epigenomics Ag Method for the analysis of cytosine methylation patterns
EP1340818A1 (en) 2002-02-27 2003-09-03 Epigenomics AG Method and nucleic acids for the analysis of a colon cell proliferative disorder
US20060099581A1 (en) * 2002-04-09 2006-05-11 Kurt Berlin Method for analysis of methylated nucleic acids
ES2272636T3 (es) 2002-06-05 2007-05-01 Epigenomics Ag Procedimiento para la determinacion cuantitativa del grado de metilac ion de citosinas en posiciones cpg.
DE10230692A1 (de) 2002-07-08 2004-02-12 Epigenomics Ag Verfahren und Nukleinsäuren für die Analyse von Methylierungsmustern innerhalb des DD3-Gens
DE10255104A1 (de) 2002-08-27 2004-03-11 Epigenomics Ag Verfahren und Nukleinsäuren für die Analyse von proliferativen Erkrankungen von Brustzellen
WO2004035803A2 (en) 2002-10-01 2004-04-29 Epigenomics Ag Method and nucleic acids for the treatment of breast cell proliferative disorders
DE10245779A1 (de) 2002-10-01 2004-04-29 Epigenomics Ag Verfahren und Nukleinsäuren für die verbesserte Behandlung von proliferativen Erkrankungen von Brustzellen
WO2004033728A2 (en) * 2002-10-11 2004-04-22 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for pcr-based clonality studies
EP1616947B1 (en) * 2003-03-28 2012-05-09 Japan as represented by Director General of National Rehabilitation Center for Persons with Disabilities METHOD OF SYNTHESIZING cDNA
WO2005001141A2 (en) 2003-06-23 2005-01-06 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
DE10338308B4 (de) 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
ES2326811T3 (es) 2003-10-09 2009-10-20 Epigenomics Ag Conversion mejorada de adn con bisulfito.
EP1609873B1 (en) 2004-06-23 2010-12-08 Epigenomics AG Method for quantification of methylated DNA
GB2424946A (en) * 2005-04-05 2006-10-11 Stratec Biomedical Systems Ag A detection system for substance binding using up-converting fluorescent probes
EP2529026B1 (en) * 2010-01-25 2013-11-13 Rd Biosciences Inc. Self-folding amplification of target nucleic acid
WO2011097424A1 (en) * 2010-02-05 2011-08-11 Siemens Healthcare Diagnostics Inc. Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions
PT2363502T (pt) * 2010-03-04 2017-05-17 Miacom Diagnostics Gmbh Fish multiplex melhorado
WO2012162161A1 (en) * 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method

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CA2603815A1 (en) 2006-10-26
AU2006236363A1 (en) 2006-10-26
WO2006113770A1 (en) 2006-10-26
JP2012105679A (ja) 2012-06-07
JP5133238B2 (ja) 2013-01-30
US10731215B2 (en) 2020-08-04
EP1871912B1 (en) 2012-02-29
ATE547537T1 (de) 2012-03-15
PT1871912E (pt) 2012-05-25
US20110059432A1 (en) 2011-03-10
PL1871912T3 (pl) 2012-07-31
AU2006236363A8 (en) 2009-03-05
ES2382746T3 (es) 2012-06-13
EP2481810A1 (en) 2012-08-01
SI1871912T1 (sl) 2012-06-29
JP5674696B2 (ja) 2015-02-25
EP1871912A1 (en) 2008-01-02
JP2008537888A (ja) 2008-10-02
CA2603815C (en) 2017-09-26
AU2006236363B2 (en) 2012-11-01
DK1871912T3 (da) 2012-05-14

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