CO5241323A1 - Linea celular productora de eritropoyetina humana recombinante . - Google Patents
Linea celular productora de eritropoyetina humana recombinante .Info
- Publication number
- CO5241323A1 CO5241323A1 CO99069968A CO99069968A CO5241323A1 CO 5241323 A1 CO5241323 A1 CO 5241323A1 CO 99069968 A CO99069968 A CO 99069968A CO 99069968 A CO99069968 A CO 99069968A CO 5241323 A1 CO5241323 A1 CO 5241323A1
- Authority
- CO
- Colombia
- Prior art keywords
- gene
- epo
- erythropoyetin
- cellular
- recombinant human
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Habiendo descrito y ejemplificado la naturaleza y objeto principal de la presente invención, así como también la manera en que la misma se puede llevar a la práctica, se declara reivindicar como de propiedad y de derechos exclusivos: UN PROCEDIMIENTO PARA LA PURIFICACIÓN DE ERITROPOYETINA HUMANA RECOMBINANTE A PARTIR DE SOBRENADANTES DE CULTIVO DE CELULAS caracterizado por incluir las siguientes etapas: precipitación diferencial; cromatografía de interacción hidrofóbica; concentración y diafiltración; cromatografía de intercambio aniónico; cromatografía de intercambio catiónico; concentración y diafiltración; cromatografía de exclusión molecular.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ARP980105609 AR008358A1 (es) | 1998-11-06 | 1998-11-06 | Celulas huesped que expresan eritropoyetina humana recombinante y un metodo para producir un polipeptido de epo |
ARP990100679 AR008363A1 (es) | 1999-02-23 | 1999-02-23 | Linea celular productora de eritropoyetina humana recombinante y la eritropoyetina humana recombinante producida por esta celula. |
Publications (1)
Publication Number | Publication Date |
---|---|
CO5241323A1 true CO5241323A1 (es) | 2003-01-31 |
Family
ID=25590826
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CO99069968A CO5241323A1 (es) | 1998-11-06 | 1999-11-05 | Linea celular productora de eritropoyetina humana recombinante . |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP1124984B1 (es) |
JP (2) | JP2002529100A (es) |
AT (1) | ATE420199T1 (es) |
AU (1) | AU1470400A (es) |
BR (1) | BR9905867A (es) |
CA (1) | CA2350335C (es) |
CO (1) | CO5241323A1 (es) |
DE (1) | DE69940267D1 (es) |
ES (1) | ES2321048T3 (es) |
WO (1) | WO2000028066A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR9905868A (pt) | 1998-11-06 | 2001-01-23 | Bio Sidus S A | Procedimento de cultivo massivo de células de mamìfero para a obtenção de eritropoetina humana, recombinante e a eritropoetina humana recombinante obtida com tal procedimento |
BR9917606A (pt) * | 1998-11-06 | 2002-12-31 | Bio Sidus S A | Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento |
US6777205B1 (en) | 1998-11-06 | 2004-08-17 | Sterrenbeld Biotechnologie North America, Inc. | Host cells expressing recombinant human erythropoietin |
ES2605358T3 (es) * | 2005-06-20 | 2017-03-14 | Cadila Healthcare Limited | Vectores de expresión y métodos para producir altos niveles de proteínas |
EP2592148B1 (en) | 2007-10-12 | 2018-09-12 | F. Hoffmann-La Roche AG | Protein expression from multiple nucleic acids |
JP5933459B2 (ja) * | 2010-02-25 | 2016-06-08 | デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド | Braf阻害剤に対する耐性を付与するbraf突然変異 |
CN103864913A (zh) | 2012-12-18 | 2014-06-18 | 联亚生技开发股份有限公司 | 重组蛋白 |
NZ756132A (en) | 2017-03-10 | 2022-02-25 | Hoffmann La Roche | Method for producing multispecific antibodies |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5010002A (en) * | 1983-01-19 | 1991-04-23 | Genentech, Inc. | Human t-PA production using vectors coding DHFR protein |
US4703008A (en) | 1983-12-13 | 1987-10-27 | Kiren-Amgen, Inc. | DNA sequences encoding erythropoietin |
NZ210501A (en) * | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
IL77081A (en) | 1984-12-04 | 1999-10-28 | Genetics Inst | AND sequence encoding human erythropoietin, a process for its preparation and a pharmacological preparation of human erythropoietin |
JPS62501010A (ja) * | 1984-12-04 | 1987-04-23 | ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド | エリトロポエチンの生産方法 |
US5641663A (en) * | 1985-11-06 | 1997-06-24 | Cangene Corporation | Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from steptomyces |
JPS62171696A (ja) * | 1986-01-23 | 1987-07-28 | Sumitomo Chem Co Ltd | ヒトエリスロポエチンの製造方法 |
JPH02485A (ja) * | 1987-08-03 | 1990-01-05 | Yuu Honshiyo | 新規なヒトインターロイキン4、該因子を発現させるための組換えベクター及びそのベクターにより形質転換された形質転換体 |
IL95064A0 (en) * | 1990-07-12 | 1991-06-10 | Yeda Res & Dev | Molecular cloning of tnf binding protein |
US5997856A (en) * | 1988-10-05 | 1999-12-07 | Chiron Corporation | Method and compositions for solubilization and stabilization of polypeptides, especially proteins |
WO1993000807A1 (en) * | 1991-07-03 | 1993-01-21 | Cryolife, Inc. | Method for stabilization of biomaterials |
JPH05194253A (ja) * | 1992-01-16 | 1993-08-03 | Kirin Brewery Co Ltd | 水溶性ポリペプチドホルモンを含む徐放性微小粒子状製剤及びその製造法 |
JP3790567B2 (ja) * | 1994-09-30 | 2006-06-28 | 武田薬品工業株式会社 | 徐放剤 |
IL118201A (en) * | 1995-05-11 | 2004-12-15 | Roche Diagnostics Gmbh | Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof |
DE19539574A1 (de) * | 1995-10-25 | 1997-04-30 | Boehringer Mannheim Gmbh | Zubereitungen und Verfahren zur Stabilisierung biologischer Materialien mittels Trocknungsverfahren ohne Einfrieren |
JPH09238688A (ja) * | 1996-03-11 | 1997-09-16 | Takeda Chem Ind Ltd | ヒトmcp−1レセプタータンパク質の製造法および用途 |
US6632648B1 (en) * | 1996-05-14 | 2003-10-14 | Elan Drug Delivery Limited | Methods of terminal sterilization of fibrinogen |
US5851984A (en) * | 1996-08-16 | 1998-12-22 | Genentech, Inc. | Method of enhancing proliferation or differentiation of hematopoietic stem cells using Wnt polypeptides |
NZ335382A (en) * | 1996-10-25 | 2001-03-30 | Searle & Co | Multi-functional chimeric hematopoietic polypeptides of IL-3, GCSF, Flt3L, EPO, stem cell factor, cmpl link with or without linker group consisting of Alanine, Glycine and/or Serine for treating autoimmune and tumour related diseases |
JPH10273447A (ja) * | 1997-01-29 | 1998-10-13 | Takeda Chem Ind Ltd | 徐放性マイクロスフィア、その製造法および用途 |
-
1999
- 1999-07-07 BR BR9905867-7A patent/BR9905867A/pt not_active Application Discontinuation
- 1999-11-05 CO CO99069968A patent/CO5241323A1/es active IP Right Grant
- 1999-11-08 JP JP2000581232A patent/JP2002529100A/ja active Pending
- 1999-11-08 AU AU14704/00A patent/AU1470400A/en not_active Abandoned
- 1999-11-08 DE DE69940267T patent/DE69940267D1/de not_active Expired - Lifetime
- 1999-11-08 CA CA2350335A patent/CA2350335C/en not_active Expired - Fee Related
- 1999-11-08 WO PCT/US1999/026238 patent/WO2000028066A1/en active Application Filing
- 1999-11-08 ES ES99971863T patent/ES2321048T3/es not_active Expired - Lifetime
- 1999-11-08 AT AT99971863T patent/ATE420199T1/de not_active IP Right Cessation
- 1999-11-08 EP EP99971863A patent/EP1124984B1/en not_active Expired - Lifetime
-
2010
- 2010-03-16 JP JP2010059896A patent/JP2010213699A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
JP2002529100A (ja) | 2002-09-10 |
BR9905867A (pt) | 2001-01-23 |
ES2321048T3 (es) | 2009-06-01 |
EP1124984A4 (en) | 2005-05-04 |
AU1470400A (en) | 2000-05-29 |
CA2350335C (en) | 2013-02-26 |
CA2350335A1 (en) | 2000-05-18 |
DE69940267D1 (de) | 2009-02-26 |
JP2010213699A (ja) | 2010-09-30 |
EP1124984A1 (en) | 2001-08-22 |
WO2000028066A1 (en) | 2000-05-18 |
EP1124984B1 (en) | 2009-01-07 |
ATE420199T1 (de) | 2009-01-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Application granted |