CO2019000828A2 - Método para detectar un mutante dentro de una población de organismos aplicando el enfoque de agrupación y división - Google Patents
Método para detectar un mutante dentro de una población de organismos aplicando el enfoque de agrupación y divisiónInfo
- Publication number
- CO2019000828A2 CO2019000828A2 CONC2019/0000828A CO2019000828A CO2019000828A2 CO 2019000828 A2 CO2019000828 A2 CO 2019000828A2 CO 2019000828 A CO2019000828 A CO 2019000828A CO 2019000828 A2 CO2019000828 A2 CO 2019000828A2
- Authority
- CO
- Colombia
- Prior art keywords
- organisms
- dpcr
- finding
- mutant
- grouping
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
En los enfoques de mejoramiento de plantas tradicionales, puede utilizarse mutagénesis química para introducir sustituciones nucleotídicas aleatoriamente en el genoma de una planta, es decir sin posibilidades de controlar los sitios de los cambios nucleotídicos. A causa de las complejidades genómicas, la probabilidad estadística es extremadamente baja en cuanto a hallar una sustitución nucleotídica predeterminada. La presente invención, sin embargo, demuestra cómo se desarrolla un uso novedoso, alternativo, de la reacción en cadena de polimerasa digital (dPCR), preferiblemente dPCR en gotas (ddPCR), para explotar el hallazgo de sustituciones nucleotídicas específicas en genes mutados. La plataforma completa comprende un método de relevamiento con una biblioteca de organismos mutagenizados, sistemas basados en PCR digital y una configuración para propagar y analizar organismos mutados identificados.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA201670485 | 2016-07-01 | ||
PCT/EP2017/065516 WO2018001884A1 (en) | 2016-07-01 | 2017-06-23 | Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach |
Publications (1)
Publication Number | Publication Date |
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CO2019000828A2 true CO2019000828A2 (es) | 2019-04-30 |
Family
ID=56681913
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CONC2019/0000828A CO2019000828A2 (es) | 2016-07-01 | 2019-01-29 | Método para detectar un mutante dentro de una población de organismos aplicando el enfoque de agrupación y división |
Country Status (25)
Country | Link |
---|---|
US (1) | US11884972B2 (es) |
EP (3) | EP3478832B1 (es) |
JP (2) | JP7208797B2 (es) |
CN (2) | CN109477087B (es) |
AR (1) | AR110103A1 (es) |
AU (3) | AU2017288960B2 (es) |
BR (1) | BR112018076429A2 (es) |
CA (1) | CA3026016A1 (es) |
CL (1) | CL2018003710A1 (es) |
CO (1) | CO2019000828A2 (es) |
CR (1) | CR20190040A (es) |
CU (1) | CU20180158A7 (es) |
DK (1) | DK3478832T3 (es) |
EA (1) | EA201892809A1 (es) |
ES (1) | ES2916221T3 (es) |
IL (1) | IL263451B (es) |
MA (3) | MA54568A (es) |
MX (2) | MX2018016110A (es) |
PE (1) | PE20190228A1 (es) |
PL (1) | PL3478832T3 (es) |
PT (1) | PT3478832T (es) |
SG (1) | SG11201811021YA (es) |
UY (1) | UY37312A (es) |
WO (1) | WO2018001884A1 (es) |
ZA (3) | ZA201808218B (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA3085987A1 (en) | 2017-12-28 | 2019-07-04 | Carlsberg A/S | Cereal plants with improved cell wall properties |
CN108913698B (zh) * | 2018-07-25 | 2021-10-26 | 安徽农业大学 | 一种与小麦穗发芽抗性/感性相关的caps标记及其应用 |
CN110724741B (zh) * | 2019-07-24 | 2020-05-19 | 艾普拜生物科技(苏州)有限公司 | 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒 |
EP3785529A1 (en) | 2019-08-28 | 2021-03-03 | Carlsberg A/S | Barley plants with altered protein content in grains |
WO2021069614A1 (en) | 2019-10-10 | 2021-04-15 | Carlsberg A/S | Methods for preparing mutant plants |
CA3167028A1 (en) | 2020-03-02 | 2021-09-10 | Carlsberg A/S | Barley plants with high limit dextrinase activity |
EP4029935A1 (en) | 2021-01-14 | 2022-07-20 | Carlsberg A/S | S. eubayanus strain and hybrids thereof |
WO2023076908A1 (en) * | 2021-10-25 | 2023-05-04 | Gt Molecular, Inc. | Off-target blocking sequences to improve target discrimination by polymerase chain reaction |
WO2023118136A1 (en) | 2021-12-22 | 2023-06-29 | BASF Agricultural Solutions Seed US LLC | Improved screening method for genome edited events |
WO2023170272A1 (en) | 2022-03-11 | 2023-09-14 | Carlsberg A/S | Modulation of protein levels |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
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US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US4351760A (en) | 1979-09-07 | 1982-09-28 | Syva Company | Novel alkyl substituted fluorescent compounds and polyamino acid conjugates |
US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
KR20000057258A (ko) | 1996-11-26 | 2000-09-15 | 크로커 사무엘 에스 | 염색체 말단으로 텔로머라제가 접근하는 것을 방지하는 억제제의 확인 |
WO1998055502A1 (en) | 1997-06-04 | 1998-12-10 | Smithkline Beecham Corporation | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
AU3221699A (en) | 1998-04-02 | 1999-10-25 | Tellus Genetic Resources, Inc. | A method for obtaining a plant with a genetic lesion in a gene sequence |
GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
EP1402038A2 (en) * | 2001-06-22 | 2004-03-31 | Syngenta Participations AG | Identification and characterization of plant genes |
GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
US7420105B2 (en) | 2004-03-11 | 2008-09-02 | Carlsberg A/S | Barley for production of flavor-stable beverage |
GB2502409B8 (en) | 2008-09-23 | 2014-10-15 | Bio Rad Laboratories | Droplet-based assay system |
US9005565B2 (en) | 2010-06-24 | 2015-04-14 | Hamid-Reza Jahangiri-Famenini | Method and apparatus for forming graphene |
US9512467B2 (en) * | 2011-03-10 | 2016-12-06 | Gen-Probe Incorporated | Methods and compositions for the selection and optimization of oligonucleotide tag sequences |
CN103492559B (zh) | 2011-04-11 | 2016-07-06 | 霍夫曼-拉罗奇有限公司 | 具有改进活性的dna聚合酶 |
ES2898460T3 (es) | 2014-02-27 | 2022-03-07 | Monsanto Technology Llc | Composiciones y procedimientos para la modificación genómica dirigida al sitio |
GB201411603D0 (en) * | 2014-06-30 | 2014-08-13 | Vela Operations Pte Ltd | Compositions for quantitative and/or semiquantitative mutation detection methods |
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2017
- 2017-06-23 CU CU2018000158A patent/CU20180158A7/es unknown
- 2017-06-23 EP EP17733800.1A patent/EP3478832B1/en active Active
- 2017-06-23 PE PE2018003333A patent/PE20190228A1/es unknown
- 2017-06-23 EP EP20163553.9A patent/EP3693458A1/en active Pending
- 2017-06-23 AU AU2017288960A patent/AU2017288960B2/en active Active
- 2017-06-23 CN CN201780041101.5A patent/CN109477087B/zh active Active
- 2017-06-23 DK DK17733800.1T patent/DK3478832T3/da active
- 2017-06-23 PT PT177338001T patent/PT3478832T/pt unknown
- 2017-06-23 EP EP20200115.2A patent/EP3792357A1/en active Pending
- 2017-06-23 IL IL263451A patent/IL263451B/en unknown
- 2017-06-23 ES ES17733800T patent/ES2916221T3/es active Active
- 2017-06-23 CR CR20190040A patent/CR20190040A/es unknown
- 2017-06-23 SG SG11201811021YA patent/SG11201811021YA/en unknown
- 2017-06-23 CN CN202210633145.XA patent/CN114959098A/zh active Pending
- 2017-06-23 MX MX2018016110A patent/MX2018016110A/es unknown
- 2017-06-23 JP JP2018569004A patent/JP7208797B2/ja active Active
- 2017-06-23 MA MA054568A patent/MA54568A/fr unknown
- 2017-06-23 EA EA201892809A patent/EA201892809A1/ru unknown
- 2017-06-23 MA MA045531A patent/MA45531A/fr unknown
- 2017-06-23 WO PCT/EP2017/065516 patent/WO2018001884A1/en unknown
- 2017-06-23 PL PL17733800T patent/PL3478832T3/pl unknown
- 2017-06-23 MA MA052594A patent/MA52594A/fr unknown
- 2017-06-23 US US16/311,450 patent/US11884972B2/en active Active
- 2017-06-23 CA CA3026016A patent/CA3026016A1/en active Pending
- 2017-06-23 BR BR112018076429A patent/BR112018076429A2/pt unknown
- 2017-06-30 AR ARP170101817A patent/AR110103A1/es unknown
- 2017-06-30 UY UY0001037312A patent/UY37312A/es not_active Application Discontinuation
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2018
- 2018-12-05 ZA ZA2018/08218A patent/ZA201808218B/en unknown
- 2018-12-18 MX MX2022014965A patent/MX2022014965A/es unknown
- 2018-12-20 CL CL2018003710A patent/CL2018003710A1/es unknown
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2019
- 2019-01-29 CO CONC2019/0000828A patent/CO2019000828A2/es unknown
- 2019-11-20 AU AU2019268102A patent/AU2019268102B2/en active Active
- 2019-11-20 AU AU2019268103A patent/AU2019268103B2/en active Active
- 2019-11-25 ZA ZA2019/07775A patent/ZA201907775B/en unknown
- 2019-11-25 ZA ZA2019/07774A patent/ZA201907774B/en unknown
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2022
- 2022-05-06 JP JP2022076471A patent/JP2022101703A/ja active Pending
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