CL2016002316A1 - Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada. - Google Patents

Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada.

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Publication number
CL2016002316A1
CL2016002316A1 CL2016002316A CL2016002316A CL2016002316A1 CL 2016002316 A1 CL2016002316 A1 CL 2016002316A1 CL 2016002316 A CL2016002316 A CL 2016002316A CL 2016002316 A CL2016002316 A CL 2016002316A CL 2016002316 A1 CL2016002316 A1 CL 2016002316A1
Authority
CL
Chile
Prior art keywords
cell
subjecting
cause
plant cell
genetic change
Prior art date
Application number
CL2016002316A
Other languages
English (en)
Inventor
Peter R Beetham
James Pearce
Gregory F W Gocal
Christian Chopke
Noel Sauer
Rosa E Segami
Jerry Mozoruk
Original Assignee
Cibus Us Llc
Cibus Europe Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cibus Us Llc, Cibus Europe Bv filed Critical Cibus Us Llc
Publication of CL2016002316A1 publication Critical patent/CL2016002316A1/es

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C12N2310/3181—Peptide nucleic acid, PNA
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/344—Position-specific modifications, e.g. on every purine, at the 3'-end
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/345—Spatial arrangement of the modifications having at least two different backbone modifications
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/346—Spatial arrangement of the modifications having a combination of backbone and sugar modifications

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Métodos y composiciones para aumentar la eficacia de la modificación génica dirigida en una célula vegetal mediante el uso de reparación génica mediada por oligonucleótidos.
CL2016002316A 2014-03-14 2016-09-14 Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada. CL2016002316A1 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201461953333P 2014-03-14 2014-03-14
US201462051579P 2014-09-17 2014-09-17
US201462075816P 2014-11-05 2014-11-05
US201462075811P 2014-11-05 2014-11-05
US201562133129P 2015-03-13 2015-03-13

Publications (1)

Publication Number Publication Date
CL2016002316A1 true CL2016002316A1 (es) 2017-05-12

Family

ID=54072512

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2016002316A CL2016002316A1 (es) 2014-03-14 2016-09-14 Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada.

Country Status (22)

Country Link
US (1) US20170081674A1 (es)
EP (2) EP3116305B1 (es)
JP (4) JP6777549B2 (es)
KR (3) KR102450868B1 (es)
CN (2) CN106455514A (es)
AU (2) AU2015229095B2 (es)
CA (2) CA3208184A1 (es)
CL (1) CL2016002316A1 (es)
DK (1) DK3116305T3 (es)
EA (1) EA201691581A1 (es)
ES (1) ES2974625T3 (es)
FI (1) FI3116305T3 (es)
HR (1) HRP20240186T1 (es)
HU (1) HUE065691T2 (es)
IL (2) IL247679B1 (es)
LT (1) LT3116305T (es)
PL (1) PL3116305T3 (es)
PT (1) PT3116305T (es)
RS (1) RS65213B1 (es)
SI (1) SI3116305T1 (es)
UA (1) UA124375C2 (es)
WO (1) WO2015139008A1 (es)

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US11542515B2 (en) 2016-02-09 2023-01-03 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair

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