CL2016002316A1 - Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada. - Google Patents
Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada.Info
- Publication number
- CL2016002316A1 CL2016002316A1 CL2016002316A CL2016002316A CL2016002316A1 CL 2016002316 A1 CL2016002316 A1 CL 2016002316A1 CL 2016002316 A CL2016002316 A CL 2016002316A CL 2016002316 A CL2016002316 A CL 2016002316A CL 2016002316 A1 CL2016002316 A1 CL 2016002316A1
- Authority
- CL
- Chile
- Prior art keywords
- cell
- subjecting
- cause
- plant cell
- genetic change
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000002068 genetic effect Effects 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 238000012239 gene modification Methods 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/315—Phosphorothioates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
- C12N2310/3181—Peptide nucleic acid, PNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/323—Chemical structure of the sugar modified ring structure
- C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/34—Spatial arrangement of the modifications
- C12N2310/344—Position-specific modifications, e.g. on every purine, at the 3'-end
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/34—Spatial arrangement of the modifications
- C12N2310/345—Spatial arrangement of the modifications having at least two different backbone modifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/34—Spatial arrangement of the modifications
- C12N2310/346—Spatial arrangement of the modifications having a combination of backbone and sugar modifications
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Métodos y composiciones para aumentar la eficacia de la modificación génica dirigida en una célula vegetal mediante el uso de reparación génica mediada por oligonucleótidos.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461953333P | 2014-03-14 | 2014-03-14 | |
US201462051579P | 2014-09-17 | 2014-09-17 | |
US201462075816P | 2014-11-05 | 2014-11-05 | |
US201462075811P | 2014-11-05 | 2014-11-05 | |
US201562133129P | 2015-03-13 | 2015-03-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
CL2016002316A1 true CL2016002316A1 (es) | 2017-05-12 |
Family
ID=54072512
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2016002316A CL2016002316A1 (es) | 2014-03-14 | 2016-09-14 | Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada. |
Country Status (22)
Country | Link |
---|---|
US (1) | US20170081674A1 (es) |
EP (2) | EP3116305B1 (es) |
JP (4) | JP6777549B2 (es) |
KR (3) | KR102450868B1 (es) |
CN (2) | CN106455514A (es) |
AU (2) | AU2015229095B2 (es) |
CA (2) | CA3208184A1 (es) |
CL (1) | CL2016002316A1 (es) |
DK (1) | DK3116305T3 (es) |
EA (1) | EA201691581A1 (es) |
ES (1) | ES2974625T3 (es) |
FI (1) | FI3116305T3 (es) |
HR (1) | HRP20240186T1 (es) |
HU (1) | HUE065691T2 (es) |
IL (2) | IL247679B1 (es) |
LT (1) | LT3116305T (es) |
PL (1) | PL3116305T3 (es) |
PT (1) | PT3116305T (es) |
RS (1) | RS65213B1 (es) |
SI (1) | SI3116305T1 (es) |
UA (1) | UA124375C2 (es) |
WO (1) | WO2015139008A1 (es) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11542515B2 (en) | 2016-02-09 | 2023-01-03 | Cibus Us Llc | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
Families Citing this family (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9957515B2 (en) | 2013-03-15 | 2018-05-01 | Cibus Us Llc | Methods and compositions for targeted gene modification |
HUE056903T2 (hu) | 2013-04-16 | 2022-04-28 | Regeneron Pharma | Patkány genom célzott módosítása |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
PT3066201T (pt) | 2013-11-07 | 2018-06-04 | Massachusetts Inst Technology | Métodos e composições relacionadas com crispr com arng reguladores |
KR102170502B1 (ko) | 2013-12-11 | 2020-10-28 | 리제너론 파마슈티칼스 인코포레이티드 | 게놈의 표적화된 변형을 위한 방법 및 조성물 |
US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
JP6727199B2 (ja) | 2014-11-21 | 2020-07-22 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | ガイドrnaのペアを使用したターゲティングによる遺伝子改変の方法及び組成物 |
JP6997623B2 (ja) * | 2014-12-12 | 2022-02-04 | エム. ウルフ、トッド | オリゴヌクレオチドを利用して細胞内の核酸を編集するための組成物および方法 |
JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したCas9蛋白質 |
EP3452055A4 (en) * | 2016-05-06 | 2019-11-06 | Tod M. Woolf | IMPROVED METHODS OF GENOME EDITING WITH AND WITHOUT PROGRAMMABLE NUCLEASES |
CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
EP3555275A1 (en) | 2016-12-14 | 2019-10-23 | Wageningen Universiteit | Thermostable cas9 nucleases |
WO2018109101A1 (en) * | 2016-12-14 | 2018-06-21 | Wageningen Universiteit | Thermostable cas9 nucleases |
JP7127942B2 (ja) * | 2016-12-22 | 2022-08-30 | キージーン ナムローゼ フェンノートシャップ | 二重鎖dnaの標的化改変の方法 |
WO2018115389A1 (en) * | 2016-12-22 | 2018-06-28 | Keygene N.V. | Methods of targeted genetic alteration in plant cells |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
WO2018149915A1 (en) * | 2017-02-15 | 2018-08-23 | Keygene N.V. | Methods of targeted genetic alteration in plant cells |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
IL306092A (en) | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
EP3676376A2 (en) | 2017-08-30 | 2020-07-08 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
CN108359646A (zh) * | 2018-01-12 | 2018-08-03 | 江苏省农业科学院 | 使植物具有除草剂抗性的ACCase突变型蛋白及其应用 |
JP6614622B2 (ja) * | 2018-04-17 | 2019-12-04 | 国立大学法人名古屋大学 | 植物ゲノム編集方法 |
US20210238612A1 (en) | 2018-07-12 | 2021-08-05 | Keygene N.V. | Type v crispr/nuclease-system for genome editing in plant cells |
CN109082416B (zh) * | 2018-08-22 | 2021-10-26 | 江苏省农业科学院 | 具有除草剂抗性的ACCase突变型基因和蛋白及其应用 |
JP2022505440A (ja) | 2018-11-01 | 2022-01-14 | キージーン ナムローゼ フェンノートシャップ | 植物細胞におけるCRISPR/Casゲノム編集のためのデュアルガイドRNA |
WO2020191241A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
CA3177481A1 (en) | 2020-05-08 | 2021-11-11 | David R. Liu | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
CN112375781B (zh) * | 2020-11-08 | 2023-05-02 | 浙江大学 | pC1300-MAS-Cas9基因编辑***在84K杨树基因编辑中的应用 |
CN115494031B (zh) * | 2021-06-18 | 2024-06-18 | 北京大学 | 一种基于CRISPR/dCas9***与寡核苷酸探针的活细胞DNA标记信号放大方法 |
CN115704040A (zh) * | 2021-08-06 | 2023-02-17 | 华东理工大学 | 基于CRISPRi和CRISPRa的转录调控***、其建立方法及应用 |
Family Cites Families (67)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4873191A (en) | 1981-06-12 | 1989-10-10 | Ohio University | Genetic transformation of zygotes |
US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
US5100792A (en) | 1984-11-13 | 1992-03-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues |
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
US5024944A (en) | 1986-08-04 | 1991-06-18 | Lubrizol Genetics, Inc. | Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species |
US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
US5608142A (en) | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
US5008200A (en) | 1988-05-08 | 1991-04-16 | United Agriseeds, Inc. | Propagating multiple whole fertile plants from immature leguminous |
US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
ES2187497T3 (es) | 1990-04-12 | 2003-06-16 | Syngenta Participations Ag | Promotores preferentemente en tejidos. |
US5204253A (en) | 1990-05-29 | 1993-04-20 | E. I. Du Pont De Nemours And Company | Method and apparatus for introducing biological substances into living cells |
US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
US5219746A (en) | 1990-07-19 | 1993-06-15 | Chris Brinegar | Ice-mediated introduction of substances into biological material |
US5399680A (en) | 1991-05-22 | 1995-03-21 | The Salk Institute For Biological Studies | Rice chitinase promoter |
US5641625A (en) | 1992-05-22 | 1997-06-24 | Isis Pharmaceuticals, Inc. | Cleaving double-stranded DNA with peptide nucleic acids |
DE4216134A1 (de) | 1991-06-20 | 1992-12-24 | Europ Lab Molekularbiolog | Synthetische katalytische oligonukleotidstrukturen |
DE69230290T2 (de) | 1991-08-27 | 2000-07-20 | Novartis Ag, Basel | Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz |
TW261517B (es) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
US5593874A (en) | 1992-03-19 | 1997-01-14 | Monsanto Company | Enhanced expression in plants |
IT230274Y1 (it) | 1993-06-11 | 1999-06-02 | Silc Spa | Pannolone assorbente sagomato per incontinenza |
US5962426A (en) | 1993-06-25 | 1999-10-05 | Yale University | Triple-helix forming oligonucleotides for targeted mutagenesis |
ATE196311T1 (de) | 1993-12-09 | 2000-09-15 | Univ Jefferson | Verbindungen und verfahren zur ortsspezifischen mutation in eukaryotischen zellen |
PT679657E (pt) | 1994-04-27 | 2003-11-28 | Novartis Ag | Nucleosidos e oligonucleotidos com grupos 2'-eter |
US5608144A (en) | 1994-08-12 | 1997-03-04 | Dna Plant Technology Corp. | Plant group 2 promoters and uses thereof |
US7285416B2 (en) | 2000-01-24 | 2007-10-23 | Gendaq Limited | Regulated gene expression in plants |
US5780296A (en) | 1995-01-17 | 1998-07-14 | Thomas Jefferson University | Compositions and methods to promote homologous recombination in eukaryotic cells and organisms |
US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
US5731181A (en) | 1996-06-17 | 1998-03-24 | Thomas Jefferson University | Chimeric mutational vectors having non-natural nucleotides |
US5888983A (en) | 1996-05-01 | 1999-03-30 | Thomas Jefferson University | Method and oligonucleobase compounds for curing diseases caused by mutations |
US5760012A (en) | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
US6072050A (en) | 1996-06-11 | 2000-06-06 | Pioneer Hi-Bred International, Inc. | Synthetic promoters |
EP0979311A1 (en) | 1997-04-30 | 2000-02-16 | Of The University Of Minnesota Regents | $i(IN VIVO) USE OF RECOMBINAGENIC OLIGONUCLEOBASES TO CORRECT GENETIC LESIONS IN HEPATOCYTES |
US6524613B1 (en) | 1997-04-30 | 2003-02-25 | Regents Of The University Of Minnesota | Hepatocellular chimeraplasty |
US7094606B2 (en) | 1997-08-05 | 2006-08-22 | Arntzen Charles J | Use of mixed duplex oligonucleotides to effect localized genetic changes in plants |
DK1017803T3 (da) * | 1997-09-26 | 2010-08-23 | Abt Holding Co | Ekspression af endogene gener ved ikke-homolog rekombination af et vektorkonstrukt med cellulært DNA |
CN1191364C (zh) | 1997-10-07 | 2005-03-02 | 巴西农业研究公司 | 获得含有外源dna的转基因豆科植物(豆科)的方法 |
DE69933713T2 (de) | 1998-02-26 | 2007-08-23 | Pioneer Hi-Bred International, Inc. | Mais alpha-tubulin 3-18 Promoter |
US6004804A (en) | 1998-05-12 | 1999-12-21 | Kimeragen, Inc. | Non-chimeric mutational vectors |
US6010907A (en) | 1998-05-12 | 2000-01-04 | Kimeragen, Inc. | Eukaryotic use of non-chimeric mutational vectors |
US7013219B2 (en) | 1999-01-12 | 2006-03-14 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US7070934B2 (en) | 1999-01-12 | 2006-07-04 | Sangamo Biosciences, Inc. | Ligand-controlled regulation of endogenous gene expression |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6271360B1 (en) | 1999-08-27 | 2001-08-07 | Valigen (Us), Inc. | Single-stranded oligodeoxynucleotide mutational vectors |
US7273923B2 (en) | 2001-01-22 | 2007-09-25 | Sangamo Biosciences, Inc. | Zinc finger proteins for DNA binding and gene regulation in plants |
US20030115641A1 (en) | 2001-07-24 | 2003-06-19 | Dobres Michael S. | Transformation of plants by electroporation of cultured explants |
US7262054B2 (en) | 2002-01-22 | 2007-08-28 | Sangamo Biosciences, Inc. | Zinc finger proteins for DNA binding and gene regulation in plants |
US7361635B2 (en) | 2002-08-29 | 2008-04-22 | Sangamo Biosciences, Inc. | Simultaneous modulation of multiple genes |
ES2735531T3 (es) | 2005-08-23 | 2019-12-19 | Univ Pennsylvania | ARN que contiene nucleósidos modificados y métodos de uso del mismo |
WO2007073149A1 (en) | 2005-12-22 | 2007-06-28 | Keygene N.V. | Alternative nucleotides for improved targeted nucleotide exchange |
DK2465340T3 (en) | 2006-01-12 | 2015-03-02 | Cibus Europe Bv | EPSPS mutants |
AU2007265434B2 (en) * | 2006-06-28 | 2012-08-02 | Cibus Europe B.V. | Fatty acid blends and uses therefor |
RU2485180C2 (ru) | 2007-06-07 | 2013-06-20 | Эгрикалча Энд Эгри-Фуд Кэнэда | Способ трансфекции и трансдукции растительных клеток |
CN102405846A (zh) | 2007-10-05 | 2012-04-11 | 因斯玛公司 | 芸苔属中突变的乙酰羟酸合酶基因 |
WO2009114321A2 (en) | 2008-03-11 | 2009-09-17 | Precision Biosciencs, Inc. | Rationally-designed meganucleases for maize genome engineering |
CN102216453B (zh) * | 2008-09-26 | 2014-02-05 | 巴斯夫农化产品有限公司 | 除草剂-抗性的ahas-突变体及使用方法 |
US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
EP2534163B1 (en) * | 2010-02-09 | 2015-11-04 | Sangamo BioSciences, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
WO2011159369A1 (en) * | 2010-06-14 | 2011-12-22 | Iowa State University Research Foundation, Inc. | Nuclease activity of tal effector and foki fusion protein |
KR101971741B1 (ko) * | 2010-07-23 | 2019-08-14 | 시그마-알드리치 컴퍼니., 엘엘씨 | 표적화 엔도뉴클레아제 및 단일-가닥 핵산을 사용하는 게놈 편집 |
WO2013028188A1 (en) | 2011-08-24 | 2013-02-28 | Cibus Us Llc | Mutated protoporphyrinogen ix oxidase (ppx) genes |
UA112969C2 (uk) * | 2010-08-03 | 2016-11-25 | Сібас Юс Ллс | Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх) |
GEP20217251B (en) | 2012-05-25 | 2021-04-26 | Charpentier Emmanuelle De Emanuel | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
RS63427B1 (sr) * | 2013-03-15 | 2022-08-31 | Cibus Us Llc | Postupci i kompozicije za povećanje efikasnosti modifikacije ciljnog gena korišćenjem genske reparacije posredovane oligonukleotidom |
-
2015
- 2015-03-14 KR KR1020167028566A patent/KR102450868B1/ko active IP Right Grant
- 2015-03-14 PL PL15761154.2T patent/PL3116305T3/pl unknown
- 2015-03-14 UA UAA201609308A patent/UA124375C2/uk unknown
- 2015-03-14 EA EA201691581A patent/EA201691581A1/ru unknown
- 2015-03-14 KR KR1020227033965A patent/KR102569558B1/ko active IP Right Grant
- 2015-03-14 EP EP15761154.2A patent/EP3116305B1/en active Active
- 2015-03-14 CN CN201580022780.2A patent/CN106455514A/zh active Pending
- 2015-03-14 HR HRP20240186TT patent/HRP20240186T1/hr unknown
- 2015-03-14 AU AU2015229095A patent/AU2015229095B2/en active Active
- 2015-03-14 HU HUE15761154A patent/HUE065691T2/hu unknown
- 2015-03-14 SI SI201531987T patent/SI3116305T1/sl unknown
- 2015-03-14 ES ES15761154T patent/ES2974625T3/es active Active
- 2015-03-14 RS RS20240194A patent/RS65213B1/sr unknown
- 2015-03-14 CN CN202311341446.6A patent/CN117867008A/zh active Pending
- 2015-03-14 JP JP2016575642A patent/JP6777549B2/ja active Active
- 2015-03-14 PT PT157611542T patent/PT3116305T/pt unknown
- 2015-03-14 US US15/126,279 patent/US20170081674A1/en not_active Abandoned
- 2015-03-14 FI FIEP15761154.2T patent/FI3116305T3/fi active
- 2015-03-14 CA CA3208184A patent/CA3208184A1/en active Pending
- 2015-03-14 LT LTEPPCT/US2015/020622T patent/LT3116305T/lt unknown
- 2015-03-14 DK DK15761154.2T patent/DK3116305T3/da active
- 2015-03-14 EP EP23214058.2A patent/EP4357452A3/en active Pending
- 2015-03-14 IL IL247679A patent/IL247679B1/en unknown
- 2015-03-14 KR KR1020237027974A patent/KR20230127356A/ko not_active Application Discontinuation
- 2015-03-14 WO PCT/US2015/020622 patent/WO2015139008A1/en active Application Filing
- 2015-03-14 CA CA2942407A patent/CA2942407C/en active Active
-
2016
- 2016-09-14 CL CL2016002316A patent/CL2016002316A1/es unknown
-
2020
- 2020-06-02 JP JP2020095870A patent/JP7047014B2/ja active Active
-
2022
- 2022-03-23 JP JP2022046763A patent/JP7293441B2/ja active Active
- 2022-04-06 AU AU2022202315A patent/AU2022202315A1/en active Pending
-
2023
- 2023-06-07 JP JP2023093667A patent/JP2023113841A/ja active Pending
- 2023-07-20 IL IL304634A patent/IL304634A/en unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11542515B2 (en) | 2016-02-09 | 2023-01-03 | Cibus Us Llc | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CL2016002316A1 (es) | Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada. | |
CL2018002140A1 (es) | Métodos y composiciones para aumentar la eficiencia de modificación genética dirigida usando reparación genética mediada por oligonucleótidos | |
DK3472325T3 (da) | Fremgangsmåde til målrettet DNA-ændring i planteceller | |
CL2015002648A1 (es) | Métodos y composiciones para aumentar la eficacia de la modificación génica dirigida utilizando reparación génica mediada por oligonucleótidos | |
UY34176A (es) | ?una construcción de adn, método para prepararla, plantas y semillas que la incluyen y métodos para generarlas?. | |
AR085533A1 (es) | Metodos de produccion de un locus de rasgos transgenicos complejo | |
AR122864A2 (es) | Variantes de lipasa y polinucleótidos que las codifican | |
CR20140538A (es) | Métodos y composiciones para la modificación de adn objetivo dirigida por arn y para la modificación de la transcripción dirigida por arn | |
AR101845A1 (es) | Anticuerpos anti-her2 e inmunoconjugados | |
UY35812A (es) | ?loci de maíz óptimos?. | |
CL2013001526A1 (es) | Métodos para incrementar la ganancia genética en una población reproductiva; método para incrementar la probabilidad de producir un individuo descendiente con un fenotipo deseado. | |
UY36113A (es) | Moléculas de ácido nucleico dre4 que confieren resistencia a plagas de coleópteros | |
BR112016028182A2 (pt) | método para produzir um fragmento de fragmento cristalizável, e, fragmento fc. | |
CL2015002650A1 (es) | Métodos y composiciones para aumentar la eficacia de la modificación génica, dirigida utilizando reparación génica mediada por oligonucleótidos. | |
UY36441A (es) | Supresión de arni parental de gen hunchback para el control de las plagas de hemípteros | |
CL2014003146A1 (es) | Método para producir virus arn monocatenario negativo; plásmido recombinante funcional en células animales, que consta de un esqueleto pss-urg; método de obtención de partículas virales; y uso para expresar arn, proteínas autógenas o exógenas al virus. | |
MX2022000516A (es) | Metodos relacionados con celulas pluripotentes. | |
NZ708061A (en) | Methods for generating genetically superior animals | |
AR092868A1 (es) | Metodo para mejorar la tolerancia al estres abiotico de plantas y plantas generadas mediante dicho metodo | |
BR112017012389A2 (pt) | supressão de rnai parental de gene kruppel para controlar pragas de coleóptero | |
CO2017011168A2 (es) | Promotor de plantas para la expresión transgénica | |
AR092688A1 (es) | Metodos y composiciones para aumentar la resistencia a nematodos en plantas | |
CL2021001708A1 (es) | Métodos para formar poliplejos | |
CL2016003149A1 (es) | Método para producir probióticos autóctonos con actividad inmunoestimulante y su uso en profilaxis contra flavobacteriosis en salmónidos. | |
UY36691A (es) | Moléculas de ácido nucleico de spt5 para controlar plagas de insectos |