EA201892809A1 - Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения - Google Patents

Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения

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EA201892809A1
EA201892809A1 EA201892809A EA201892809A EA201892809A1 EA 201892809 A1 EA201892809 A1 EA 201892809A1 EA 201892809 A EA201892809 A EA 201892809A EA 201892809 A EA201892809 A EA 201892809A EA 201892809 A1 EA201892809 A1 EA 201892809A1
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organisms
dpcr
pools
population
association
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EA201892809A
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English (en)
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Тони Вендт
Оле Олсен
Сорен Кнудсен
Ханне Сесил Томсен
Александр Страйбек
Биргитт Скадхауг
Магнус Вольфарт Расмуссен
Массимилиано Карчофи
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Карлсберг А/С
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
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  • Chemical & Material Sciences (AREA)
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Abstract

В традиционных подходах к селекции растений химический мутагенез можно использовать для случайного введения нуклеотидных замен в геном растения, т.е. без возможности контролировать сайты нуклеотидных изменений. Когда дело касается поиска заданной нуклеотидной замены, статистическая вероятность является крайне низкой из-за сложности генома. В настоящем изобретении, тем не менее, продемонстрировано, как разработать новое альтернативное применение цифровой полимеразной цепной реакции (dPCR), предпочтительно капельной dPCR (ddPCR), для обнаружения конкретных нуклеотидных замен в мутантных генах. Вся платформа включает способ скрининга с библиотекой подвергнутых мутагенезу организмов, системы на основе цифровой ПЦР и установку для размножения и анализа выявленных мутантных организмов.
EA201892809A 2016-07-01 2017-06-23 Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения EA201892809A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201670485 2016-07-01
PCT/EP2017/065516 WO2018001884A1 (en) 2016-07-01 2017-06-23 Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

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EA201892809A1 true EA201892809A1 (ru) 2019-06-28

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US (1) US11884972B2 (ru)
EP (3) EP3478832B1 (ru)
JP (2) JP7208797B2 (ru)
CN (2) CN109477087B (ru)
AR (1) AR110103A1 (ru)
AU (3) AU2017288960B2 (ru)
BR (1) BR112018076429A2 (ru)
CA (1) CA3026016A1 (ru)
CL (1) CL2018003710A1 (ru)
CO (1) CO2019000828A2 (ru)
CR (1) CR20190040A (ru)
CU (1) CU20180158A7 (ru)
DK (1) DK3478832T3 (ru)
EA (1) EA201892809A1 (ru)
ES (1) ES2916221T3 (ru)
IL (1) IL263451B (ru)
MA (3) MA54568A (ru)
MX (2) MX2018016110A (ru)
PE (1) PE20190228A1 (ru)
PL (1) PL3478832T3 (ru)
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CN110724741B (zh) * 2019-07-24 2020-05-19 艾普拜生物科技(苏州)有限公司 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
EP3785529A1 (en) 2019-08-28 2021-03-03 Carlsberg A/S Barley plants with altered protein content in grains
WO2021069614A1 (en) 2019-10-10 2021-04-15 Carlsberg A/S Methods for preparing mutant plants
CA3167028A1 (en) 2020-03-02 2021-09-10 Carlsberg A/S Barley plants with high limit dextrinase activity
EP4029935A1 (en) 2021-01-14 2022-07-20 Carlsberg A/S S. eubayanus strain and hybrids thereof
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WO2023170272A1 (en) 2022-03-11 2023-09-14 Carlsberg A/S Modulation of protein levels

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MX2018016110A (es) 2019-05-30
CN109477087B (zh) 2022-06-24
CR20190040A (es) 2019-05-09
AR110103A1 (es) 2019-02-27
AU2017288960A1 (en) 2019-01-17
EP3478832A1 (en) 2019-05-08
PT3478832T (pt) 2022-05-19
PE20190228A1 (es) 2019-02-13
AU2017288960B2 (en) 2019-12-12
BR112018076429A2 (pt) 2019-10-01
EP3693458A1 (en) 2020-08-12
EP3792357A1 (en) 2021-03-17
AU2019268103B2 (en) 2020-03-12
IL263451A (en) 2019-01-31
UY37312A (es) 2018-02-28
CN109477087A (zh) 2019-03-15
CN114959098A (zh) 2022-08-30
CL2018003710A1 (es) 2019-03-22
ZA201907775B (en) 2022-09-28
MA54568A (fr) 2021-10-27
WO2018001884A1 (en) 2018-01-04
JP2022101703A (ja) 2022-07-06
PL3478832T3 (pl) 2022-06-06
AU2019268102A1 (en) 2019-12-12
IL263451B (en) 2022-08-01
JP7208797B2 (ja) 2023-01-19
US11884972B2 (en) 2024-01-30
ZA201907774B (en) 2022-07-27
ES2916221T3 (es) 2022-06-29
MX2022014965A (es) 2023-01-11
SG11201811021YA (en) 2019-01-30
AU2019268103A1 (en) 2019-12-12
CU20180158A7 (es) 2019-08-06
EP3478832B1 (en) 2022-02-23
MA45531A (fr) 2019-05-08
DK3478832T3 (da) 2022-05-30
CA3026016A1 (en) 2018-01-04
MA52594A (fr) 2021-03-17
US20190194723A1 (en) 2019-06-27
JP2019524083A (ja) 2019-09-05
CO2019000828A2 (es) 2019-04-30
AU2019268102B2 (en) 2020-03-05
ZA201808218B (en) 2020-02-26

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