CO2017010631A2 - Método para purificación de proteína - Google Patents
Método para purificación de proteínaInfo
- Publication number
- CO2017010631A2 CO2017010631A2 CONC2017/0010631A CO2017010631A CO2017010631A2 CO 2017010631 A2 CO2017010631 A2 CO 2017010631A2 CO 2017010631 A CO2017010631 A CO 2017010631A CO 2017010631 A2 CO2017010631 A2 CO 2017010631A2
- Authority
- CO
- Colombia
- Prior art keywords
- monomeric
- domain
- human
- antibody
- protein purification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/624—Disulfide-stabilized antibody (dsFv)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención proporciona un método para recuperar un anticuerpo que contiene un dominio VH3 humano en forma monomérica. En particular, la presente invención proporciona un nuevo método que permite la recuperación de anticuerpos monoméricos que contienen el dominio VH3 humano a partir de una mezcla que contiene formas monoméricas y multiméricas del anticuerpo. El particular, el método reclamado comprende: a) aplicar una mezcla que comprende un anticuerpo que contiene el dominio VH3 humano en forma monomérica y multimérica a un material de cromatografía de proteína A y b) la recuperación del anticuerpo que contiene el dominio VH3 humano, en forma monomérica.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1506868.7A GB201506868D0 (en) | 2015-04-22 | 2015-04-22 | Method for protein purification |
| PCT/EP2016/058774 WO2016169992A1 (en) | 2015-04-22 | 2016-04-20 | Method for protein purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO2017010631A2 true CO2017010631A2 (es) | 2018-01-05 |
Family
ID=53299017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CONC2017/0010631A CO2017010631A2 (es) | 2015-04-22 | 2017-10-18 | Método para purificación de proteína |
Country Status (19)
| Country | Link |
|---|---|
| US (2) | US10927164B2 (es) |
| EP (1) | EP3286205A1 (es) |
| JP (2) | JP6866300B2 (es) |
| KR (1) | KR102675400B1 (es) |
| CN (1) | CN107636012B (es) |
| AR (1) | AR104358A1 (es) |
| AU (1) | AU2016251223B2 (es) |
| BR (1) | BR112017022073A2 (es) |
| CA (1) | CA2983057A1 (es) |
| CL (1) | CL2017002614A1 (es) |
| CO (1) | CO2017010631A2 (es) |
| EA (1) | EA201792328A1 (es) |
| GB (1) | GB201506868D0 (es) |
| IL (1) | IL254883B (es) |
| MA (1) | MA41941A (es) |
| MX (1) | MX2017013333A (es) |
| MY (1) | MY189854A (es) |
| SG (1) | SG11201708256YA (es) |
| WO (1) | WO2016169992A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013081540A1 (en) * | 2011-11-30 | 2013-06-06 | Ge Healthcare Bio-Sciences Ab | Affinity chromatography matrix |
| GB201506869D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
| GB201506870D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
| CN108085358A (zh) * | 2018-02-08 | 2018-05-29 | 保定冀中药业有限公司 | 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法 |
| EP4007771A1 (en) | 2019-08-02 | 2022-06-08 | UCB Biopharma SRL | Methods for purifying antibodies |
| GB201919058D0 (en) * | 2019-12-20 | 2020-02-05 | Ucb Biopharma Sprl | Multi-specific antibodies |
| AU2020414489A1 (en) * | 2019-12-26 | 2022-07-14 | Abl Bio Inc. | Method for purifying biologically active peptide by using protein a affinity chromatography |
| GB202109246D0 (en) * | 2021-06-28 | 2021-08-11 | Cytiva Bioprocess R & D Ab | A method of separating bispecific antibodies |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| CA2374013A1 (en) | 1999-05-15 | 2000-11-23 | University Of California, San Diego | Protein a based binding domains with desirable activities |
| WO2002059264A2 (en) * | 2000-12-29 | 2002-08-01 | Bio-Technology General Corp. | Specific human antibodies for selective cancer therapy |
| US20060257972A1 (en) * | 2003-03-31 | 2006-11-16 | Takashi Ishihara | Purification of human monoclonal antibody and human polyclonal antibody |
| AU2009282234A1 (en) * | 2008-08-14 | 2010-02-18 | Merck Sharp & Dohme Corp. | Methods for purifying antibodies using protein a affinity chromatography |
| HUE042114T2 (hu) * | 2009-02-17 | 2019-06-28 | Ucb Biopharma Sprl | Humán OX40-re specifikus ellenanyag-molekulák |
| EP2635599A1 (en) * | 2010-11-01 | 2013-09-11 | DSM IP Assets B.V. | Single unit ion exchange chromatography antibody purification |
| US8859726B2 (en) | 2010-11-29 | 2014-10-14 | Ge Healthcare Bio-Sciences Ab | Affinity chromatography matrix |
| UA112203C2 (uk) * | 2011-11-11 | 2016-08-10 | Юсб Фарма С.А. | Злитий білок біоспецифічного антитіла, який зв'язується з ox40 людини та сироватковим альбуміном людини |
| DK2776466T3 (da) | 2011-11-11 | 2017-11-20 | Ucb Biopharma Sprl | Albuminbindende antistoffer og bindingsfragmenter deraf |
| BR112014019579A2 (pt) * | 2012-02-10 | 2019-10-15 | Genentech, Inc | Anticorpo de cadeia única, polinucleotídeo, vetor, célula hospedeira, método de produção de um anticorpo de cadeia única, heteromultímero e método de produção do heteromultímero |
| GB201223276D0 (en) * | 2012-12-21 | 2013-02-06 | Ucb Pharma Sa | Antibodies and methods of producing same |
| NZ720161A (en) * | 2013-11-04 | 2022-07-29 | Ichnos Sciences SA | Production of t cell retargeting hetero-dimeric immunoglobulins |
| GB201506870D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
| GB201506869D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
-
2015
- 2015-04-22 GB GBGB1506868.7A patent/GB201506868D0/en not_active Ceased
-
2016
- 2016-04-20 AU AU2016251223A patent/AU2016251223B2/en active Active
- 2016-04-20 KR KR1020177033687A patent/KR102675400B1/ko active IP Right Grant
- 2016-04-20 EA EA201792328A patent/EA201792328A1/ru unknown
- 2016-04-20 MY MYPI2017703933A patent/MY189854A/en unknown
- 2016-04-20 MA MA041941A patent/MA41941A/fr unknown
- 2016-04-20 CA CA2983057A patent/CA2983057A1/en active Pending
- 2016-04-20 SG SG11201708256YA patent/SG11201708256YA/en unknown
- 2016-04-20 CN CN201680028589.3A patent/CN107636012B/zh active Active
- 2016-04-20 EP EP16717915.9A patent/EP3286205A1/en active Pending
- 2016-04-20 US US15/566,231 patent/US10927164B2/en active Active
- 2016-04-20 WO PCT/EP2016/058774 patent/WO2016169992A1/en active Application Filing
- 2016-04-20 MX MX2017013333A patent/MX2017013333A/es unknown
- 2016-04-20 BR BR112017022073A patent/BR112017022073A2/pt active Search and Examination
- 2016-04-20 JP JP2017555232A patent/JP6866300B2/ja active Active
- 2016-04-22 AR ARP160101122A patent/AR104358A1/es unknown
-
2017
- 2017-10-03 IL IL254883A patent/IL254883B/en unknown
- 2017-10-16 CL CL2017002614A patent/CL2017002614A1/es unknown
- 2017-10-18 CO CONC2017/0010631A patent/CO2017010631A2/es unknown
-
2021
- 2021-01-07 JP JP2021001584A patent/JP2021066734A/ja not_active Abandoned
- 2021-02-17 US US17/177,236 patent/US20210171609A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MA41941A (fr) | 2018-02-28 |
| US20210171609A1 (en) | 2021-06-10 |
| SG11201708256YA (en) | 2017-11-29 |
| KR102675400B1 (ko) | 2024-06-13 |
| BR112017022073A2 (pt) | 2018-08-14 |
| MX2017013333A (es) | 2018-02-19 |
| JP2021066734A (ja) | 2021-04-30 |
| KR20170139131A (ko) | 2017-12-18 |
| US10927164B2 (en) | 2021-02-23 |
| IL254883B (en) | 2021-09-30 |
| AU2016251223A1 (en) | 2017-10-26 |
| CL2017002614A1 (es) | 2018-04-27 |
| IL254883A0 (en) | 2017-12-31 |
| EA201792328A1 (ru) | 2018-05-31 |
| EP3286205A1 (en) | 2018-02-28 |
| US20180100007A1 (en) | 2018-04-12 |
| JP6866300B2 (ja) | 2021-04-28 |
| AR104358A1 (es) | 2017-07-12 |
| AU2016251223B2 (en) | 2021-12-09 |
| CA2983057A1 (en) | 2016-10-27 |
| MY189854A (en) | 2022-03-14 |
| JP2018520089A (ja) | 2018-07-26 |
| CN107636012A (zh) | 2018-01-26 |
| WO2016169992A1 (en) | 2016-10-27 |
| CN107636012B (zh) | 2022-08-02 |
| GB201506868D0 (en) | 2015-06-03 |
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