CN2914091Y - Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine - Google Patents
Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine Download PDFInfo
- Publication number
- CN2914091Y CN2914091Y CN 200620004307 CN200620004307U CN2914091Y CN 2914091 Y CN2914091 Y CN 2914091Y CN 200620004307 CN200620004307 CN 200620004307 CN 200620004307 U CN200620004307 U CN 200620004307U CN 2914091 Y CN2914091 Y CN 2914091Y
- Authority
- CN
- China
- Prior art keywords
- test paper
- mdma
- crystal methamphetamine
- monoclonal antibody
- dioxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a test paper for fast methylene dioxy methyl amphetamine (MDMA) detection and pertains to colloidal gold immunodetection field. The test paper is composed of a base plate, a suction plate, a nitrocellulose membrane, an Epratuzumab gold labeled pad and a glass fiber sample imbibing layer. The utility model is a test paper used in MDMA detection. The nitrocellulose membrane is positioned on the middle base plate and is provided with a MDMA synthetic immunogen test wire and a polyclonal antibody control wire. One end of the base plate is the suction plate and the other end is the sample imbibing layer. Two ends of the nitrocellulose membrane are overlapped and connected with the suction plate and a monoclonal antibody gold labeled pad respectively. The sample imbibing layer is pressed on the monoclonal antibody gold labeled pad and the test paper is made by the method of colloidal gold immunity competition. The theory is to utilize the specific binding reaction and immunochromatography technique of antigen antibody, with specific chromogenic results on test paper. The utility model has the advantages of high sensitivity, good specificity, good repeatability, high precision, simple operation, fast and accurate detection, with no need for complicated equipments.
Description
Technical field
The present invention is a kind of test paper that utilizes the colloid gold immune analytical approach to detect methylene dioxygen crystal methamphetamine (MDMA).
Background technology
Methylene dioxygen crystal methamphetamine (MDMA) formal name used at school is: N, and a-dimethyl-3-4-methylene dioxy phenyl ethylamine, the molecule formula weight is C
11H
15O
2N=193.Pure product are white powder, belong to the amphetamine excitant, and chemical constitution similar " ice " poison is commonly called as " K ", " L ", dancing outreach, " Adam ", " disco biscuit ", " happy ball ", " selfless " etc., and the strong unreal effect that causes is arranged.
The amphetamine excitant can suppress the activity of fourth amino oxidase, make the chemical transmitter matter of adrenaline and adrenergic nerve, the destruction of norepinephrine is slowed down, make the refreshing generation excitement of each maincenter under cerebral cortex and the cortex, cause palpitating speed, heavy dose can cause that heart suppresses even death.Its symptom mainly shows as dysphoria, headache, dizziness, tinnitus, nervously breathes hard, feels sick, vomiting, perspiration, heart rate are accelerated, throb with fear, face sometimes flush, mydriasis, expiratory dyspnea, cyanosis, blood pressure fall after rising, at last because of respiratory failure or circulatory failure death.Because of it has habituation, dependence, so national departments concerned is classified them as drugs ranks.
To the detection method such as the gas chromatography (GC) of methylene dioxygen crystal methamphetamine (MDMA), gas chromatography/mass spectrometry (GC/MS), high performance liquid chromatography existing reports such as (HPLC).Characteristics such as high performance capillary electrophoresis is the analytical approach that development in recent years is got up, and it has, and separating power is strong, separation efficiency is high, analysis time is short, and required sample and quantity of solvent are little.The external existing micella kapillary electrokinetic chromatography method etc. of utilizing, but the required time is longer, therefore needs a kind of detection method fast, easily.
The principle of colloid gold test paper is to utilize the specific binding reaction and the immunochromatography technique of antigen-antibody, utilize specific chromogenic reaction on test paper, to show the result of test accurately, have highly sensitive, high specificity, good reproducibility, the degree of accuracy height, simple to operate, detect characteristics fast and accurately, need not complex apparatus.
Summary of the invention
The object of the invention has been to provide a kind of test paper that utilizes the colloid gold immune analytical approach to detect methylene dioxygen crystal methamphetamine (MDMA).
In order to reach described purpose, the present invention is achieved by the following technical solutions:
Detecting test paper base plate middle part at methylene dioxygen crystal methamphetamine (MDMA) is nitrocellulose filter, a methylene dioxygen crystal methamphetamine (MDMA) synthetic immunogen test wire and an anti-mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the glass fiber sample layer, the nitrocellulose filter two ends overlap mutually with anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody gold mark pad with water accepting layer respectively and are connected, and are pressed with the glass fiber sample layer on anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody gold mark pad.
Methylene dioxygen crystal methamphetamine (MDMA) is detected the sample layer of test paper and put into detected urine (liquid level must not surpass the MAX line) because the capillarity sample will move to the water accepting layer end along test strips, when moving to anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody gold mark pad, methylene dioxygen crystal methamphetamine (MDMA) in the sample and anti-methylene dioxygen crystal methamphetamine (MDMA) gold mark probe generation idiosyncrasy, when moving to when being fixed with methylene dioxygen crystal methamphetamine (MDMA) synthetic immunogen test wire, lose itself and methylene dioxygen crystal methamphetamine (MDMA) synthetic immunogen binding site owing to the antibody in anti-methylene dioxygen crystal methamphetamine (MDMA) the monoclonal antibody gold mark pad can combine with methylene dioxygen crystal methamphetamine (MDMA) in the sample, so its collaurum can not be stranded on the test wire; On the contrary, if the methylene dioxygen crystal methamphetamine (MDMA) of the antibodies in not filling up in the sample with anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody gold mark, anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody gold mark probe will with methylene dioxygen crystal methamphetamine (MDMA) the synthetic immunogen generation specific bond on the test wire, collaurum is stranded on the test wire, immune competition law principle that Here it is.So methylene dioxygen crystal methamphetamine (MDMA) content just is inversely proportional in the shade of test wire and the sample.The setting boundary value is 500ng/ml, two red lines occur and represents that MDMA is lower than 500ng/ml in the urine sample, and is negative; A red line occurs and represent MDMA value 500ng/ml beyond the mark in the urine sample, positive.
When moving to anti-mouse polyclonal antibody control line, no matter in the sample methylene dioxygen crystal methamphetamine (MDMA) content what, gold mark probe all can combine delay with the mouse IgG that is coated on the nitrocellulose filter, it is red that control line is shown.So control line does not have, and colour band produces then representative operation wrong (during detection, the sample liquid level surpasses the MAX line) or test paper is expired.
With after the detection paper urine, with its test wire with detect colour code and carry out colorimetric, be judged as the positive by shade, suspicious or negative, and then determine survey whether methylene dioxygen crystal methamphetamine (MDMA) content exceeds standard in the urine.Get urine detection with containing methylene dioxygen crystal methamphetamine (MDMA) reference substance, set boundary value 500ng/ml.
Owing to adopt technique scheme, the test paper and the detection method thereof of detection methylene dioxygen crystal methamphetamine provided by the present invention (MDMA) have such beneficial effect, it is high specificity, highly sensitive, easily store, need not the technical skill personnel operation, do not need instrument and equipment, and readability as a result.
Description of drawings
Figure one is the main TV structure figure that methylene dioxygen crystal methamphetamine (MDMA) detects test paper.
Figure two detects the sectional structure chart of overlooking of test paper for methylene dioxygen crystal methamphetamine (MDMA).
Figure three is shown as positive findings figure when test paper is applied to test for methylene dioxygen crystal methamphetamine (MDMA) detects.
Figure four is shown as negative findings figure when test paper is applied to test for methylene dioxygen crystal methamphetamine (MDMA) detects.
Embodiment
1. use activation fat method, haptens methylene dioxygen crystal methamphetamine (MDMA) is prepared methylene dioxygen crystal methamphetamine (MDMA) synthetic immunogen in conjunction with bovine serum albumin(BSA) (BSA).
2. anti-methylene dioxygen crystal methamphetamine (MDMA) MONOCLONAL ANTIBODIES SPECIFIC FOR.
(1) with methylene dioxygen crystal methamphetamine (MDMA) the synthetic immunogen immune mouse for preparing.
(2) extract mouse ascites, separation and purification is to obtain anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody.
The preparation of collaurum and with the combining of anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody.
(1) 10% chlorauride (0.5ml)+10% trisodium citrate (0.75ml)+deionized water (500ml) → collaurum.
(2) collaurum 500ml+0.1M phosphate buffer pH7.4 (50ml)+anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody → anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody collaurum.
(3) standby on anti-methylene dioxygen crystal methamphetamine (MDMA) monoclonal antibody collaurum+thieving paper → golden labelled antibody absorption thieving paper.
4. select nitrocellulose filter covering and locate, utilize capillary theory that collaurum is moved towards the substrate place as substrate.
5. extraordinary film-making machine system film: machine is special, utilizes computer control transmission speed, guarantees that the antibody amount of bag quilt on the per unit film equates.
Control line is selected for use: sheep anti-mouse igg, concentration 2-10mg/ml
Test wire is selected for use: methylene dioxygen crystal methamphetamine (MDMA) synthetic immunogen, concentration 2-10mg/ml
6. combination:
(1) plastic strip+film+collaurum pad
(2) slitting
7. using method
(1) removes external packing, take out reagent strip.Reagent strip is inserted in the urine sample, and urine interface thing surpasses " Max " line on the paper slip.As be kit, then urine is splashed in the sample well.
Take out test strips after (2) 15 seconds or continue to be inserted in the urine observations in the time of 5 minutes; As be kit, direct observations in viewport after 5 minutes then.
(3) result judges: a red line only occurs in the control zone positive; As two red line occurring, then negative at detection zone and control zone.Red line does not appear in the control zone, and surperficial test paper is invalid.
8. points for attention
(1) only supplies qualitative screening.
(2) dilution and unclean urine sample or misoperation all can produce error result.
(3) sealing is preserved, and uses in 12 months in the term of validity.
Claims (5)
1. the colloid gold test paper of a fast detecting methylene dioxygen crystal methamphetamine is characterized in that being made up of base plate (7), water sucting plate (1), nitrocellulose filter (2), methylene dioxygen crystal methamphetamine monoclonal antibody gold mark pad (5), glass fiber sample liquid-adsorption layer (6); The base plate middle part is a nitrocellulose filter, a test wire (4) and a sheep anti mouse polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
2. the colloid gold test paper of a kind of fast detecting methylene dioxygen crystal methamphetamine according to claim 1 is characterized in that control line is to be made into by sheep anti mouse polyclonal antibody bag.
3. the colloid gold test paper of a kind of fast detecting methylene dioxygen crystal methamphetamine according to claim 1 is characterized in that test wire is to be made into by methylene dioxygen crystal methamphetamine molecule synthesis immunogene bag.
4. the colloid gold test paper of a kind of fast detecting methylene dioxygen crystal methamphetamine according to claim 1 is characterized in that gold mark pad is as colloid gold label by methylene dioxygen crystal methamphetamine molecule monoclonal antibody.
5. test strips according to claim 1 is characterized in that thieving paper double-layer water absorbing paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004307 CN2914091Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004307 CN2914091Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2914091Y true CN2914091Y (en) | 2007-06-20 |
Family
ID=38168692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620004307 Expired - Lifetime CN2914091Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2914091Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078715B (en) * | 2007-06-28 | 2010-12-08 | 艾博生物医药(杭州)有限公司 | Standard solution and its formulation method and uses |
| CN102288760A (en) * | 2010-06-18 | 2011-12-21 | 北京库尔科技有限公司 | Dimethylenedioxyamphetamine detection kit and preparation method thereof |
| CN102539764A (en) * | 2010-12-27 | 2012-07-04 | 北京库尔科技有限公司 | Pethidine hydrochloride detection kit and preparation method thereof |
| CN102539765A (en) * | 2010-12-29 | 2012-07-04 | 北京宝瑞源科技孵化有限公司 | Triazolam detection kit and preparation method thereof |
| CN107121552A (en) * | 2017-04-20 | 2017-09-01 | 江南大学 | One plant is secreted the hybridoma cell strain ZY 2G5 3 of 3,4- methylene benzylene chloride propylamine monoclonal antibodies and its applied |
-
2006
- 2006-02-22 CN CN 200620004307 patent/CN2914091Y/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078715B (en) * | 2007-06-28 | 2010-12-08 | 艾博生物医药(杭州)有限公司 | Standard solution and its formulation method and uses |
| CN102288760A (en) * | 2010-06-18 | 2011-12-21 | 北京库尔科技有限公司 | Dimethylenedioxyamphetamine detection kit and preparation method thereof |
| CN102539764A (en) * | 2010-12-27 | 2012-07-04 | 北京库尔科技有限公司 | Pethidine hydrochloride detection kit and preparation method thereof |
| CN102539765A (en) * | 2010-12-29 | 2012-07-04 | 北京宝瑞源科技孵化有限公司 | Triazolam detection kit and preparation method thereof |
| CN107121552A (en) * | 2017-04-20 | 2017-09-01 | 江南大学 | One plant is secreted the hybridoma cell strain ZY 2G5 3 of 3,4- methylene benzylene chloride propylamine monoclonal antibodies and its applied |
| CN107121552B (en) * | 2017-04-20 | 2018-09-21 | 江南大学 | One plant of hybridoma cell strain ZY-2G5-3 for secreting 3,4- methylene benzylene chloride propylamine monoclonal antibodies and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN2914091Y (en) | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine | |
| CN102099683B (en) | Device and method for separating and analyzing blood | |
| CN102778558B (en) | Lateral reciprocating immunochromatography method and diagnosis test strip and device based on lateral reciprocating immunochromatography method | |
| CN102207507A (en) | Semi-quantitative detecting test paper of cardiac troponin and preparation method thereof | |
| CN101881769A (en) | Colloidal gold immunochromatographic test strip for detecting shrimp allergen and preparation method thereof | |
| US5714336A (en) | Process and test kit for determining free active compounds in biological fluids | |
| CN2914093Y (en) | Colloidal gold test paper for rapidly detecting ketamine | |
| CN2935155Y (en) | Colloidal gold test paper for quick detecting ***e | |
| CN2914088Y (en) | Colloidal gold test paper for rapidly detecting phencyclidine | |
| CN104237521B (en) | Three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol and preparation method thereof | |
| CN105445454A (en) | Quantifiable immunochromatography device | |
| CN101458252A (en) | Dairy antibiotic rapid detection kit | |
| CN205003159U (en) | Microcystic toxins - LR fluorescence immunity quick quantitative detection card | |
| CN203101391U (en) | Gold-labeled immunochromatography detecting system | |
| CN2938101Y (en) | Collidal gold test paper for quickly investigating BZO | |
| CN202101996U (en) | Quick test card for phthalic ester | |
| CN110346580A (en) | A kind of glycosylated hemoglobin fluorescence is affine immunochromatographytest test kit and detection method | |
| CN201607444U (en) | Chromatograph test paper for fast diagnosing occult blood | |
| CN2935156Y (en) | Colloidal gold test paper for quick detecting bhang | |
| CN2914090Y (en) | Colloidal gold test paper for rapidly detecting amphetamine | |
| CN2938102Y (en) | Collidal gold test paper for quick investigating tricyclic antidepressant | |
| CN103389384A (en) | Miltenberger blood group antibody detection test strip and detection method thereof | |
| CN215986100U (en) | High efficiency lipoprotein associated phospholipase A2 check out test set | |
| CN207571147U (en) | For the test strips of fluorescence immune chromatography method detection | |
| CN2914092Y (en) | Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20070620 |
|
| EXPY | Termination of patent right or utility model |