CN205003159U - Microcystic toxins - LR fluorescence immunity quick quantitative detection card - Google Patents
Microcystic toxins - LR fluorescence immunity quick quantitative detection card Download PDFInfo
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- CN205003159U CN205003159U CN201520619533.8U CN201520619533U CN205003159U CN 205003159 U CN205003159 U CN 205003159U CN 201520619533 U CN201520619533 U CN 201520619533U CN 205003159 U CN205003159 U CN 205003159U
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Abstract
The application discloses microcystic toxins - LR fluorescence immunity quick quantitative detection card includes the test paper strip and cup joints at the outer shell of test paper strip, the note include the bottom plate and fix sample pad on the bottom plate in order, combine to fill up, reaction membrane and pads that absorb water, the one end that combines to fill up is inlayed between sample pads and bottom plate, it is epimembranal that the other end is located the reaction, react epimembranal and be equipped with detection line and matter accuse line, under the one end of reaction membrane is located and combines to fill up, under the other end is located the absorption pad, be equipped with on the shell with sample pad matched with goes up the appearance hole, be equipped with on the shell with detection line and matter accuse line matched with reserve the window. The utility model discloses a fluorescent label antibody technique combines with the ELISA technique, is applicable to the microcystic toxins - LR in the on -the -spot quick quantitative detection drinking water, and the sample need not the pretreatment, has easy operation, convenient and fast, advantage such as accuracy as a result.
Description
Technical field
The utility model relates to field of water quality detection, specifically, relates to the fluorescent mark immunity chromatography test card of a kind of Quantitative detection microcystin in drinking water-LR.
Background technology
Along with global water body eutrophication degree aggravates, the generation of blue-green alga bloom and red tide increases gradually, consequent secondary metabolite Microcystin (Microcystins, MCs), to harm one of great environmental problem becoming global concern of water body environment and human health.Microcystin is a series of seven peptide monocycle liver poison poison, there is ring texture and interval double bond, thus have suitable stability in structure.Microcystin can the activity of strong inhibition phosphoprotein phosphatase, simultaneously it or strong liver neoplasm promoter, is one of arch-criminal of China's onset of liver cancer rate sharp increase in recent years.
Representative in Microcystin is microcapsule algae toxin (Microcystin-LR), microcystin in drinking water-LR content is restricted to 1 μ g/L by China's drinking water sanitary standard (GB5749-2006), and the enforcement of this standard detects drinking water safety has higher requirement.Both at home and abroad for the detection of Microcystin mainly with mouse poisoned technique, high performance liquid chromatography, and Enzyme-linked Immunosorbent Assay (microwell plate) method is main, mouse poisoned technique due to costly, the restriction such as animal protection seldom uses; And high performance liquid chromatography cannot realize scene and detect fast; Enzyme linked immunosorbent assay (microwell plate) can Site Detection, but needs 1.5 hours detection time, and pre-treatment is more complicated, cannot reflect the safety of on-the-spot potable water in time.
The immunochromatography technique that the eighties in 20th century grows up becomes the study hotspot of food security field of fast detection in recent years, this analytical technology is fast simple to operate, analysis result is easy to recognition, and need not specialized equipment, but immuno-chromatographic test paper strip adopts collaurum method substantially, there are differences between batches large, can not realize when detecting microcystin in drinking water quantitatively, the shortcomings such as sensitivity is low.Fluorescein is utilized to rise as the immuno-chromatographic test paper strip of tracer-labelling antibody in recent years, the method is without the need to preparing nm of gold, only need to coordinate the fluorescence detector together researched and developed, can accomplish low cost, highly sensitive monitoring field potable water, sample is without the need to pre-treatment, therefore convenient and swift, detection time only needs 10 minutes, by the exact level of microcapsule algae toxin in instrument calculation sample, and the generation of prevention potable water poisoning.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of fluorescent mark immunity chromatography test card for Quantitative detection microcystin in drinking water-LR, realizes on-the-spot Quantitative detection for the microcapsule algae toxin in potable water.
For solving the problems of the technologies described above, the utility model provides a kind of microcapsule algae toxin fluorescence immunoassay Quantitative detection card, comprise test strips and be socketed in the outer field shell of test strips, described paper slip comprises base plate, and the sample pad be fixed in turn on base plate, pad, reaction film and adsorptive pads, one end of pad is embedded between sample pad and base plate, the other end is positioned on reaction film, reaction film is provided with detection line and nature controlling line, under one end of reaction film is positioned at pad, under the other end is positioned at absorption pad, described shell is provided with the loading hole of matching with described sample pad, described shell is provided with the reserved window matched with described detection line and nature controlling line.
Preferably, described base plate, be PVC offset plate further, described sample pad, pad, reaction film and adsorptive pads are bonded on described PVC offset plate in turn.
Preferably, described loading hole is positioned at directly over the bottom of described sample pad, for quantitative loading.
Preferably, the spacing of described detection line and nature controlling line is 4 ± 0.05mm.
Preferably, described microcapsule algae toxin fluorescence immunoassay Quantitative detection card, the rectangle test card further for matching with the sample cell of fluorescence detector.
Compared with prior art, microcapsule algae toxin fluorescence immunoassay Quantitative detection card described in the utility model, reaches following effect:
The utility model adopts fluorescent-labeled antibody technology to be combined with Enzyme-multiplied immune technique, be applicable to the microcapsule algae toxin in on-the-spot Quantitative detection potable water, sample is without the need to pre-treatment, have simple, convenient quick, the advantage such as result is accurate, detection limit can meet detection microcystin in drinking water-LR limit standard 1 μ g/L.In addition, induced fluorescence, than visible light sensitivity high hundred times, can reduce the requirement that antagonist is tired greatly.
Accompanying drawing explanation
Accompanying drawing described herein is used to provide further understanding of the present utility model, forms a part of the present utility model, and schematic description and description of the present utility model, for explaining the utility model, is not formed improper restriction of the present utility model.In the accompanying drawings:
Fig. 1 is the structural representation of test strips of the present utility model;
Fig. 2 is the structural representation after the utility model assembling;
Wherein: 1-sample pad; 2-pad; 3-reaction film; 4-base plate;
5-adsorptive pads; 6-detection line; 7-nature controlling line; 8-loading hole; 9-shell;
10-reserved window.
Embodiment
As employed some vocabulary to censure specific components in the middle of instructions and claim.Those skilled in the art should understand, and hardware manufacturer may call same assembly with different noun.This specification and claims are not used as with the difference of title the mode distinguishing assembly, but are used as the criterion of differentiation with assembly difference functionally." comprising " as mentioned in the middle of instructions and claim is in the whole text an open language, therefore should be construed to " comprise but be not limited to "." roughly " refer to that in receivable error range, those skilled in the art can solve the technical problem within the scope of certain error, reach described technique effect substantially.In addition, " couple " word and comprise directly any and indirectly electric property coupling means at this.Therefore, if describe a first device in literary composition to be coupled to one second device, then represent described first device and directly can be electrically coupled to described second device, or be indirectly electrically coupled to described second device by other devices or the means that couple.Instructions subsequent descriptions is for implementing better embodiment of the present utility model, and right described description is to illustrate for the purpose of rule of the present utility model, and is not used to limit scope of the present utility model.Protection domain of the present utility model is when being as the criterion depending on the claims person of defining.
Composition graphs 1 and Fig. 2, present embodiments provide a kind of microcapsule algae toxin fluorescence immunoassay Quantitative detection card, as can be seen from the figure the microcapsule algae toxin fluorescence immunoassay Quantitative detection card that the application provides is rectangular test card, is the rectangle test card matched with the sample cell of fluorescence detector.
Microcapsule algae toxin fluorescence immunoassay Quantitative detection card comprises test strips and is socketed in the outer field shell 9 of test strips, and described paper slip comprises base plate 4 and is fixed on sample pad 1, pad 2, reaction film 3 and the adsorptive pads 5 on base plate 4 in turn:
Base plate 4 in the present embodiment is PVC offset plate, and described sample pad 1, pad 2, reaction film 3 and adsorptive pads 5 are bonded on described PVC offset plate in turn.Certain base plate 4 here can also be other material, but will ensure that it has good hydrophobicity, does not namely absorb sample liquids.
Described sample pad 1 is the position of sample solution loading;
One end of pad 2 is embedded between sample pad 1 and base plate 4, and the other end is positioned on reaction film 3;
Reaction film 3 is provided with detection line 6 and nature controlling line 7, and one end of reaction film 3 is positioned at pad 2 times, under the other end is positioned at absorption pad; Detection line 6 is fixed with microcapsule algae toxin-BSA, nature controlling line 7 is fixed with sheep anti-mouse igg; The spacing of described detection line 6 and nature controlling line 7 is 4 ± 0.05mm.
Described shell 9 is provided with the loading hole 8 of matching with described sample pad 1, and described shell 9 is provided with the reserved window 10 matched with described detection line 6 and nature controlling line 7, and reserved window 10 is more directly perceived, convenient when reading numerical values.Described loading hole 8 is positioned at directly over the bottom of described sample pad 1, for quantitative loading.Outside detection line 6 and nature controlling line 7 are apparent in by the reserved window 10 of shell 9, shell 9, except for except quantitative loading and fixing test strips, also reads numeral for inserting fluorescence detector.
When testing, the shell 9 of test card being inserted the sample cell of fluorescence detector, just can read the fluorescent value of sample.
Described sample pad is sample solution loading position, and pad is provided with fluorescence-antibody complex, and reaction film is provided with detection line and nature controlling line.Detection line is fixed with microcystins-LR-BSA, nature controlling line is fixed with sheep anti-mouse igg.Principle of work of the present utility model is Immune competition method, that is: microcapsule algae toxin antibody is carried out fluorescence to add and be designated as fluorescence-antibody complex, be fixed on pad, previously prepared good detection is former is fixed on reaction film with capture antibody respectively as detection line and nature controlling line, is assembled into test strips; When testing sample adds well, fluorescence-the antibody complex of toxin in sample on pad is combined, inhibit the former combination of the detection on fluorescence-antibody complex and reaction film, thus attenuation is played to the fluorescence signal value of detection line, by typical curve, can content of toxins in calculation sample.Wherein whether nature controlling line plays Indicator Paper bar and effectively acts in this fluorescent mark immunity chromatograph test strip.No matter this sample is negative or positive, and nature controlling line all can detect fluorescence signal value, otherwise this test strips is invalid.
The application utilizes antibody fluorescence labelling technique, be labeled immunoglobulin compound by fluorescent marker and antibody coupling, specific antibody and antigen-reactive and antibody and capture antibody react, form the detection line of fluorescence display and nature controlling line, whether are the detection line of its fluorescence display and the existence of nature controlling line susceptible of proof measured object.During this test strips configuration induced fluorescence detector, the typical curve that the concentration of measured object can be formulated by series standard solution detection line fluorescence display parameter calculates.
Below in conjunction with accompanying drawing, the utility model is described in further detail, but not as to restriction of the present utility model.
Above-mentioned explanation illustrate and describes some preferred embodiments of the present utility model, but as previously mentioned, be to be understood that the utility model is not limited to the form disclosed by this paper, should not regard the eliminating to other embodiments as, and can be used for other combinations various, amendment and environment, and can in utility model contemplated scope described herein, changed by the technology of above-mentioned instruction or association area or knowledge.And the change that those skilled in the art carry out and change do not depart from spirit and scope of the present utility model, then all should in the protection domain of the utility model claims.
Claims (4)
1. a microcapsule algae toxin fluorescence immunoassay Quantitative detection card, it is characterized in that, described microcapsule algae toxin fluorescence immunoassay Quantitative detection card, for the rectangle test card matched with the sample cell of fluorescence detector, comprise test strips and be socketed in the outer field shell of test strips, described shell is used for quantitative loading and fixing described test strips, also read numeral for inserting fluorescence detector, described paper slip comprises base plate, and the sample pad be fixed in turn on base plate, pad, reaction film and adsorptive pads, one end of pad is embedded between sample pad and base plate, the other end is positioned on reaction film, reaction film is provided with detection line and nature controlling line, under one end of reaction film is positioned at pad, under the other end is positioned at absorption pad, described shell is provided with the loading hole of matching with described sample pad, described shell is provided with the reserved window matched with described detection line and nature controlling line, outside described detection line and nature controlling line are apparent in by the reserved window of described shell.
2. microcapsule algae toxin fluorescence immunoassay Quantitative detection card according to claim 1, it is characterized in that, described base plate, is PVC offset plate further, and described sample pad, pad, reaction film and adsorptive pads are bonded on described PVC offset plate in turn.
3. microcapsule algae toxin fluorescence immunoassay Quantitative detection card according to claim 1, it is characterized in that, described loading hole is positioned at directly over the bottom of described sample pad, for quantitative loading.
4. microcapsule algae toxin fluorescence immunoassay Quantitative detection card according to claim 1, is characterized in that, the spacing of described detection line and nature controlling line is 4 ± 0.05mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520619533.8U CN205003159U (en) | 2015-08-17 | 2015-08-17 | Microcystic toxins - LR fluorescence immunity quick quantitative detection card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520619533.8U CN205003159U (en) | 2015-08-17 | 2015-08-17 | Microcystic toxins - LR fluorescence immunity quick quantitative detection card |
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| CN205003159U true CN205003159U (en) | 2016-01-27 |
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| CN201520619533.8U Expired - Fee Related CN205003159U (en) | 2015-08-17 | 2015-08-17 | Microcystic toxins - LR fluorescence immunity quick quantitative detection card |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106990246A (en) * | 2017-04-01 | 2017-07-28 | 天津农学院 | A kind of microcysin LR enzyme-linked immunologic detecting kit |
| CN109406784A (en) * | 2017-08-16 | 2019-03-01 | 上海雄图生物科技有限公司 | A kind of detection microcapsule phycotoxin MC-LR time-resolved fluoroimmunoassay quantifies POCT test strips |
| CN109444306A (en) * | 2018-12-24 | 2019-03-08 | 赵文晋 | A kind of method that efficient liquid phase fluorescence chromatography detects microcapsule algae toxin in water |
| CN109655435A (en) * | 2018-11-20 | 2019-04-19 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | A kind of fluorescent quenching test paper and the preparation method and application thereof detecting okadaic acid |
-
2015
- 2015-08-17 CN CN201520619533.8U patent/CN205003159U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106990246A (en) * | 2017-04-01 | 2017-07-28 | 天津农学院 | A kind of microcysin LR enzyme-linked immunologic detecting kit |
| CN109406784A (en) * | 2017-08-16 | 2019-03-01 | 上海雄图生物科技有限公司 | A kind of detection microcapsule phycotoxin MC-LR time-resolved fluoroimmunoassay quantifies POCT test strips |
| CN109655435A (en) * | 2018-11-20 | 2019-04-19 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | A kind of fluorescent quenching test paper and the preparation method and application thereof detecting okadaic acid |
| CN109655435B (en) * | 2018-11-20 | 2021-08-03 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | Fluorescence quenching test paper for detecting okadaic acid and preparation method and application thereof |
| CN109444306A (en) * | 2018-12-24 | 2019-03-08 | 赵文晋 | A kind of method that efficient liquid phase fluorescence chromatography detects microcapsule algae toxin in water |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160127 Termination date: 20210817 |
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| CF01 | Termination of patent right due to non-payment of annual fee |