CN2914088Y - Colloidal gold test paper for rapidly detecting phencyclidine - Google Patents
Colloidal gold test paper for rapidly detecting phencyclidine Download PDFInfo
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- CN2914088Y CN2914088Y CN 200620004304 CN200620004304U CN2914088Y CN 2914088 Y CN2914088 Y CN 2914088Y CN 200620004304 CN200620004304 CN 200620004304 CN 200620004304 U CN200620004304 U CN 200620004304U CN 2914088 Y CN2914088 Y CN 2914088Y
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- pcp
- test paper
- monoclonal antibody
- hog
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Abstract
The utility model relates to a test paper for fast phencyclidine (PCP) detection and pertains to colloidal gold immunodetection field. The test paper is composed of a base plate, a suction plate, a nitrocellulose membrane, an Epratuzumab gold labeled pad and a glass fiber sample imbibing layer. The utility model is a test paper used in PCP detection. The nitrocellulose membrane is positioned on the middle base plate and is provided with a PCP synthetic immunogen test wire and a polyclonal antibody control wire. One end of the base plate is the suction plate and the other end is the sample imbibing layer. Two ends of the nitrocellulose membrane are overlapped and connected with the suction plate and a monoclonal antibody gold labeled pad respectively. The sample imbibing layer is pressed on the monoclonal antibody gold labeled pad and the test paper is made by the method of colloidal gold immunity competition. The theory is to utilize the specific binding reaction and immunochromatography technique of antigen antibody, with specific chromogenic results on test paper. The utility model has the advantages of high sensitivity, good specificity, good repeatability, high precision, simple operation, fast and accurate detection, with no need for complicated equipments.
Description
Technical field
The present invention is a kind of test paper that utilizes the colloid gold immune analytical approach to detect Hog (PCP).
Background technology
Hog is also referred to as the Pu Sipu agent, is chemosynthesis, and pulverulent solids is a kind of anesthesiophore unreal class medicine that causes.1956 synthetic first by chemical laboratory of detroit ,u.s.a.
Hog produces multiple effect by brain and the nervous centralis activity that influences the people to human body.Low dose is taken and the sedation effect similar to most of inhibitor can be occurred; Median dose is taken then sensigenous obstacle, shows as analgesia or anaesthesia phenomenon; If acute poisoning symptoms such as the large dose oral administration Hog will be hallucinated and faint from fear, stupor even death.After heavy dose of medication 1-2 hour, the drug addict begin to occur emotional lability, excited restless, lose pain, nerve numb and self-induction weightlessness, notice can not be concentrated then, produces the disturbance of thought, engender illusion, therefore going back of having cause attack behavior or autotomy.Data shows, the suicide that causes by taking Hog, behavior such as kill a person is much more than other psychedelic.Take behind the Hog because of the descend death toll that causes of confusion of thinking, insensitive, judgment and autocontrol force is many more than the death toll that chemical toxicity caused of this drugs itself, and much the cause of death is avoidable fully In the view of ordinary person.Be drowned in the shallow water beach because of confusion of thinking, autocontrol force are too poor as the user; Because of insensitive, unable again the taking one's bearings of pain disappearance are burned alive or the like in the event of fire that can escape fully.On physiology, suck, symptoms such as benzene injection Perhexiline person has n and V, a large amount of perspiration, nystagmus, diplopia.As other psychedelic, Hog has certain psychologic dependence, but finds no physical dependence.Belong to national drugs ranks.
At present, method and instrument to Hog (PCP) fast detecting are more, mainly be to rely on methods such as gas chromatography (GS), high performance liquid chromatography (HPLC), mass spectrum (MS) and Capillary Electrophoresis to detect, but exist instrument costliness, detection time oversize, and need the technical professional to operate, therefore be badly in need of a kind of can be fast, accurately, detection method easily.
The principle of colloid gold test paper is to utilize the specific binding reaction and the immunochromatography technique of antigen-antibody, utilize specific chromogenic reaction on test paper, to show the result of test accurately, have highly sensitive, high specificity, good reproducibility, the degree of accuracy height, simple to operate, detect characteristics fast and accurately, need not complex apparatus.
Summary of the invention
The object of the invention has been to provide a kind of test paper and detection method thereof of utilizing the colloid gold immune analytical approach to detect Hog (PCP).
In order to reach described purpose, the present invention is achieved by the following technical solutions:
Detecting test paper base plate middle part at Hog (PCP) is nitrocellulose filter, a Hog (PCP) synthetic immunogen test wire and an anti-mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the glass fiber sample layer, the nitrocellulose filter two ends overlap mutually with anti-Hog (PCP) monoclonal antibody gold mark pad with water accepting layer respectively and are connected, and are pressed with the glass fiber sample layer on anti-Hog (PCP) monoclonal antibody gold mark pad.
Hog (PCP) is detected the sample layer of test paper and put into detected urine (liquid level must not surpass the MAX line) because the capillarity sample will move to the water accepting layer end along test strips, when moving to anti-Hog (PCP) monoclonal antibody gold mark pad, the spy is taken place with anti-Hog (PCP) gold mark probe and is opened reaction in the Hog in the sample (PCP), when moving to when being fixed with Hog (PCP) synthetic immunogen test wire, lose itself and Hog (PCP) synthetic immunogen binding site owing to the antibody in anti-Hog (PCP) the monoclonal antibody gold mark pad can combine with Hog in the sample (PCP), so its collaurum can not be stranded on the test wire; On the contrary, if the Hog (PCP) of the antibodies in not filling up in the sample with anti-Hog (PCP) monoclonal antibody gold mark, anti-Hog (PCP) monoclonal antibody gold mark probe will with Hog (PCP) the synthetic immunogen generation specific bond on the test wire, collaurum is stranded on the test wire, immune competition law principle that Here it is.So Hog in the shade of test wire and the sample (PCP) content just is inversely proportional to.The setting boundary value is 25ng/ml, two red lines occur and represents that PCP is lower than 25ng/ml in the urine sample, and is negative; A red line occurs and represent PCP value 25ng/ml beyond the mark in the urine sample, positive.
When moving to anti-mouse polyclonal antibody control line, no matter in the sample Hog (PCP) content what, gold mark probe all can combine delay with the mouse IgG that is coated on the nitrocellulose filter, it is red that control line is shown.So control line does not have, and colour band produces then representative operation wrong (during detection, the sample liquid level surpasses the MAX line) or test paper is expired.
With after the detection paper urine, with its test wire with detect colour code and carry out colorimetric, be judged as the positive by shade, suspicious or negative, and then determine the Hog in the urine of surveying (PCP) content whether exceed standard.Get urine detection with containing Hog (PCP) reference substance, the setting boundary value is 25ng/ml.
Because adopt technique scheme, the test paper and the detection method thereof of detection Hog provided by the present invention (PCP) have such beneficial effect, i.e. high specificity, highly sensitive, easily store, need not the technical skill personnel operation, do not need instrument and equipment, and readability as a result.
Description of drawings
Figure one is the main TV structure figure that Hog (PCP) detects test paper.
Figure two detects the sectional structure chart of overlooking of test paper for Hog (PCP).
Figure three is shown as positive findings figure when test paper is applied to test for Hog (PCP) detects.
Figure four is shown as negative findings figure when test paper is applied to test for Hog (PCP) detects.
Embodiment
1. use activation fat method, haptens Hog (PCP) is prepared Hog (PCP) synthetic immunogen in conjunction with bovine serum albumin(BSA) (BSA).
2. anti-Hog (PCP) MONOCLONAL ANTIBODIES SPECIFIC FOR.
(1) with Hog (PCP) the synthetic immunogen immune mouse for preparing.
(2) extract mouse ascites, separation and purification is to obtain anti-Hog (PCP) monoclonal antibody.
The preparation of collaurum and with the combining of anti-Hog (PCP) monoclonal antibody.
(1) 10% chlorauride (0.5ml)+10% trisodium citrate (0.75ml)+deionized water (500ml) → collaurum.
(2) collaurum 500ml+0.1M phosphate buffer pH7.4 (50ml)+anti-Hog (PCP) monoclonal antibody → anti-Hog (PCP) monoclonal antibody collaurum.
(3) standby on anti-Hog (PCP) monoclonal antibody collaurum+thieving paper → golden labelled antibody absorption thieving paper.
4. select nitrocellulose filter covering and locate, utilize capillary theory that collaurum is moved towards the substrate place as substrate.
5. extraordinary film-making machine system film: machine is special, utilizes computer control transmission speed, guarantees that the antibody amount of bag quilt on the per unit film equates.
Control line is selected for use: sheep anti-mouse igg, concentration 2-10mg/ml
Test wire is selected for use: Hog (PCP) synthetic immunogen, concentration 2-10mg/ml
6. combination:
(1) plastic strip+film+collaurum pad
(2) slitting
7. using method
(1) removes external packing, take out reagent strip.Reagent strip is inserted in the urine sample, and urine interface thing surpasses " Max " line on the paper slip.As be kit, then urine is splashed in the sample well.
Take out test strips after (2) 15 seconds or continue to be inserted in the urine observations in the time of 5 minutes; As be kit, direct observations in viewport after 5 minutes then.
(3) result judges: a red line only occurs in the control zone positive; As two red line occurring, then negative at detection zone and control zone.Red line does not appear in the control zone, and surperficial test paper is invalid.
8. points for attention
(1) only supplies qualitative screening.
(2) dilution and unclean urine sample or misoperation all can produce error result.
(3) sealing is preserved, and uses in 12 months in the term of validity.
Claims (5)
1. the colloid gold test paper of a fast detecting PCP is characterized in that being made up of base plate (7), water sucting plate (1), nitrocellulose filter (2), PCP monoclonal antibody gold mark pad (5), glass fiber sample liquid-adsorption layer (6); The base plate middle part is a nitrocellulose filter, a test wire (4) and a sheep anti mouse polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
2. the colloid gold test paper of a kind of fast detecting PCP according to claim 1 is characterized in that control line is to be made into by sheep anti mouse polyclonal antibody bag.
3. the colloid gold test paper of a kind of fast detecting PCP according to claim 1 is characterized in that test wire is to be made into by PCP molecule synthesis immunogene bag.
4. the colloid gold test paper of a kind of fast detecting PCP according to claim 1 is characterized in that gold mark pad is as colloid gold label by PCP molecule monoclonal antibody.
5. test strips according to claim 1 is characterized in that thieving paper double-layer water absorbing paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004304 CN2914088Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting phencyclidine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004304 CN2914088Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting phencyclidine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2914088Y true CN2914088Y (en) | 2007-06-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620004304 Expired - Lifetime CN2914088Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting phencyclidine |
Country Status (1)
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| CN (1) | CN2914088Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539764A (en) * | 2010-12-27 | 2012-07-04 | 北京库尔科技有限公司 | Pethidine hydrochloride detection kit and preparation method thereof |
| CN102928594A (en) * | 2011-12-27 | 2013-02-13 | 北京宝瑞源科技孵化有限公司 | Phencyclidine detection kit and preparation method thereof |
| CN105669866A (en) * | 2015-11-20 | 2016-06-15 | 艾博生物医药(杭州)有限公司 | Hybridoma cell strain of phencyclidine (PCP) monoclonal antibody and preparation method thereof |
| CN111443205A (en) * | 2020-02-25 | 2020-07-24 | 古镜科技(深圳)有限公司 | Quintuplet test paper for detecting abuse of mental drugs and preparation thereof |
| CN114137232A (en) * | 2021-11-19 | 2022-03-04 | 佛山墨赛生物技术有限公司 | Latex microsphere immunoassay test strip, kit and method |
-
2006
- 2006-02-22 CN CN 200620004304 patent/CN2914088Y/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539764A (en) * | 2010-12-27 | 2012-07-04 | 北京库尔科技有限公司 | Pethidine hydrochloride detection kit and preparation method thereof |
| CN102928594A (en) * | 2011-12-27 | 2013-02-13 | 北京宝瑞源科技孵化有限公司 | Phencyclidine detection kit and preparation method thereof |
| CN102928594B (en) * | 2011-12-27 | 2015-11-25 | 北京宝瑞源科技孵化有限公司 | phencyclidine detection kit and preparation method thereof |
| CN105669866A (en) * | 2015-11-20 | 2016-06-15 | 艾博生物医药(杭州)有限公司 | Hybridoma cell strain of phencyclidine (PCP) monoclonal antibody and preparation method thereof |
| CN111443205A (en) * | 2020-02-25 | 2020-07-24 | 古镜科技(深圳)有限公司 | Quintuplet test paper for detecting abuse of mental drugs and preparation thereof |
| CN114137232A (en) * | 2021-11-19 | 2022-03-04 | 佛山墨赛生物技术有限公司 | Latex microsphere immunoassay test strip, kit and method |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20070620 |
|
| EXPY | Termination of patent right or utility model |