CN2914092Y - Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone - Google Patents
Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone Download PDFInfo
- Publication number
- CN2914092Y CN2914092Y CN 200620004310 CN200620004310U CN2914092Y CN 2914092 Y CN2914092 Y CN 2914092Y CN 200620004310 CN200620004310 CN 200620004310 CN 200620004310 U CN200620004310 U CN 200620004310U CN 2914092 Y CN2914092 Y CN 2914092Y
- Authority
- CN
- China
- Prior art keywords
- test paper
- oxycodone
- hydroxyl dihydrocodeinone
- monoclonal antibody
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a test paper for fast Oxycodone detection and pertains to colloidal gold immunodetection field. The test paper is composed of a base plate, a suction plate, a nitrocellulose membrane, an Epratuzumab gold labeled pad and a glass fiber sample imbibing layer. The utility model is a test paper used in Oxycodone detection. The nitrocellulose membrane is positioned on the middle base plate and is provided with a Oxycodone synthetic immunogen test wire and a polyclonal antibody control wire. One end of the base plate is the suction plate and the other end is the sample imbibing layer. Two ends of the nitrocellulose membrane are overlapped and connected with the suction plate and a monoclonal antibody gold labeled pad respectively. The sample imbibing layer is pressed on the monoclonal antibody gold labeled pad and the test paper is made by the method of colloidal gold immunity competition. The theory is to utilize the specific binding reaction and immunochromatography technique of antigen antibody, with specific chromogenic results on test paper. The utility model has the advantages of high sensitivity, good specificity, good repeatability, high precision, simple operation, fast and accurate detection, with no need for complicated equipments.
Description
Technical field
The present invention is a kind of test paper that utilizes the colloid gold immune analytical approach to detect hydroxyl dihydrocodeinone (Oxycodone).
Background technology
The hydroxyl dihydrocodeinone is an opium kind analgesics, is used to the treatment of pain of poisoning, and is usually used in treating migraines, is the medicine of the sportsman of the national sportsman association of United States Olympic Committee regulation taboo when participating in competition, and can make people's spirit excited after taking.
Hydroxyl dihydrocodeinone controlled release tablet OxyContin (Oxycontin) is called as " native heroin " (hillbilly heroin).The user wears into powder with this tablet for alleviating pain, changes its slowly-releasing characteristics, adopts snuffing or injection then.Cause to have habituation, country classifies them as second level drugs ranks.
At present, method and instrument to hydroxyl dihydrocodeinone (Oxycodone) fast detecting are more, mainly be to rely on methods such as gas chromatography (GS), high performance liquid chromatography (HPLC), mass spectrum (MS) and Capillary Electrophoresis to detect, but exist instrument costliness, detection time oversize, and need the technical professional to operate, therefore be badly in need of a kind of can be fast, accurately, detection method easily.
The principle of colloid gold test paper is to utilize the specific binding reaction and the immunochromatography technique of antigen-antibody, utilize specific chromogenic reaction on test paper, to show the result of test accurately, have highly sensitive, high specificity, good reproducibility, the degree of accuracy height, simple to operate, detect characteristics fast and accurately, need not complex apparatus.
Summary of the invention
The object of the invention has been to provide a kind of test paper that utilizes the colloid gold immune analytical approach to detect hydroxyl dihydrocodeinone (Oxycodone).
In order to reach described purpose, the present invention is achieved by the following technical solutions:
Detecting test paper base plate middle part at hydroxyl dihydrocodeinone (Oxycodone) is nitrocellulose filter, a hydroxyl dihydrocodeinone (Oxycodone) synthetic immunogen test wire and an anti-mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the glass fiber sample layer, the nitrocellulose filter two ends overlap mutually with anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody gold mark pad with water accepting layer respectively and are connected, and are pressed with the glass fiber sample layer on anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody gold mark pad.
Hydroxyl dihydrocodeinone (Oxycodone) is detected the sample layer of test paper and put into detected urine (liquid level must not surpass the MAX line) because the capillarity sample will move to the water accepting layer end along test strips, when moving to anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody gold mark pad, hydroxyl dihydrocodeinone (Oxycodone) in the sample and anti-hydroxyl dihydrocodeinone (Oxyxodone) gold mark probe generation idiosyncrasy, when moving to when being fixed with hydroxyl dihydrocodeinone (Oxycodone) synthetic immunogen test wire, lose itself and hydroxyl dihydrocodeinone (Oxycodone) synthetic immunogen binding site owing to the antibody in anti-hydroxyl dihydrocodeinone (Oxycodone) the monoclonal antibody gold mark pad can combine with hydroxyl dihydrocodeinone (Oxycodone) in the sample, so its collaurum can not be stranded on the test wire; On the contrary, if the hydroxyl dihydrocodeinone (Oxycodone) of the antibodies in not filling up in the sample with anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody gold mark, anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody gold mark probe will with hydroxyl dihydrocodeinone (Oxycodone) the synthetic immunogen generation specific bond on the test wire, collaurum is stranded on the test wire, immune competition law principle that Here it is.So hydroxyl dihydrocodeinone (Oxycodone) content just is inversely proportional in the shade of test wire and the sample.The setting boundary value is 100ng/ml, two red lines occur and represents that hydroxyl dihydrocodeinone (Oxycodone) is lower than 100ng/ml in the urine sample, and is negative; A red line occurs and represent hydroxyl dihydrocodeinone (Oxycodone) value 100ng/ml beyond the mark in the urine sample, positive.
When moving to anti-mouse polyclonal antibody control line, no matter in the sample hydroxyl dihydrocodeinone (Oxycodone) content what, gold mark probe all can combine delay with the mouse IgG that is coated on the nitrocellulose filter, it is red that control line is shown.So control line does not have, and colour band produces then representative operation wrong (during detection, the sample liquid level surpasses the MAX line) or test paper is expired.
With after the detection paper urine, with its test wire with detect colour code and carry out colorimetric, be judged as the positive by shade, suspicious or negative, and then determine survey whether hydroxyl dihydrocodeinone (Oxycodone) content exceeds standard in the urine.Get urine detection with containing hydroxyl dihydrocodeinone (Oxycodone) reference substance, the boundary value of setting is 100ng/ml.
Because adopt technique scheme, the test paper and the detection method thereof of detection hydroxyl dihydrocodeinone provided by the present invention (Oxycodone) have such beneficial effect, i.e. high specificity, highly sensitive, easily store, need not the technical skill personnel operation, do not need instrument and equipment, and readability as a result.
Description of drawings
Figure one is the main TV structure figure that hydroxyl dihydrocodeinone (Oxycodone) detects test paper.
Figure two detects the sectional structure chart of overlooking of test paper for hydroxyl dihydrocodeinone (Oxycodone).
Figure three is shown as positive findings figure when test paper is applied to test for hydroxyl dihydrocodeinone (Oxycodone) detects.
Figure four is shown as negative findings figure when test paper is applied to test for hydroxyl dihydrocodeinone (Oxycodone) detects.
Embodiment
1. use activation fat method, haptens hydroxyl dihydrocodeinone (Oxycodone) is prepared hydroxyl dihydrocodeinone (Oxycodone) synthetic immunogen in conjunction with bovine serum albumin(BSA) (BSA).
2. anti-hydroxyl dihydrocodeinone (Oxycodone) MONOCLONAL ANTIBODIES SPECIFIC FOR.
(1) with hydroxyl dihydrocodeinone (Oxycodone) the synthetic immunogen immune mouse for preparing.
(2) extract mouse ascites, separation and purification is to obtain anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody.
The preparation of collaurum and with the combining of anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody.
(1) 10% chlorauride (0.5ml)+10% trisodium citrate (0.75ml)+deionized water (500ml) → collaurum.
(2) collaurum 500ml+0.1M phosphate buffer pH7.4 (50ml)+anti-hydroxyl dihydrocodeinone (Oxycodone)
Monoclonal antibody → anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody collaurum.
(3) standby on anti-hydroxyl dihydrocodeinone (Oxycodone) monoclonal antibody collaurum+thieving paper → golden labelled antibody absorption thieving paper.
4. select nitrocellulose filter covering and locate, utilize capillary theory that collaurum is moved towards the substrate place as substrate.
5. extraordinary film-making machine system film; Machine is special, utilizes computer control transmission speed, guarantees that the antibody amount of bag quilt on the per unit film equates.
Control line is selected for use: sheep anti-mouse igg, concentration 2-10mg/ml
Test wire is selected for use: hydroxyl dihydrocodeinone (Oxycodone) synthetic immunogen, concentration 2-10mg/ml
6. combination:
(1) plastic strip+film+collaurum pad
(2) slitting
7. using method
(1) removes external packing, take out reagent strip.Reagent strip is inserted in the urine sample, and urine interface thing surpasses " Max " line on the paper slip.As be kit, then urine is splashed in the sample well.
Take out test strips after (2) 15 seconds or continue to be inserted in the urine observations in the time of 5 minutes; As be kit, direct observations in viewport after 5 minutes then.
(3) result judges: a red line only occurs in the control zone positive: as two red line occurring at detection zone and control zone, then negative.Red line does not appear in the control zone, and surperficial test paper is invalid.
8. points for attention
(1) only supplies qualitative screening.
(2) dilution and unclean urine sample or misoperation all can produce error result.
(3) sealing is preserved, and uses in 12 months in the term of validity.
Claims (5)
1. the colloid gold test paper of a fast detecting hydroxyl dihydrocodeinone is characterized in that being made up of base plate (7), water sucting plate (1), nitrocellulose filter (2), hydroxyl dihydrocodeinone monoclonal antibody gold mark pad (5), glass fiber sample liquid-adsorption layer (6); The base plate middle part is a nitrocellulose filter, a test wire (4) and a sheep anti mouse polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
2. the colloid gold test paper of a kind of fast detecting hydroxyl dihydrocodeinone according to claim 1 is characterized in that control line is to be made into by sheep anti mouse polyclonal antibody bag.
3. the colloid gold test paper of a kind of fast detecting hydroxyl dihydrocodeinone according to claim 1 is characterized in that test wire is to be made into by hydroxyl dihydrocodeinone synthetic immunogen bag.
4. the colloid gold test paper of a kind of fast detecting hydroxyl dihydrocodeinone according to claim 1 is characterized in that gold mark pad is as colloid gold label by hydroxyl dihydrocodeinone monoclonal antibody.
5. test strips according to claim 1 is characterized in that thieving paper double-layer water absorbing paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004310 CN2914092Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620004310 CN2914092Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2914092Y true CN2914092Y (en) | 2007-06-20 |
Family
ID=38168693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620004310 Expired - Lifetime CN2914092Y (en) | 2006-02-22 | 2006-02-22 | Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2914092Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114137207A (en) * | 2021-11-19 | 2022-03-04 | 佛山墨赛生物技术有限公司 | Test strip, kit and method for quantitative detection of oxycodone |
-
2006
- 2006-02-22 CN CN 200620004310 patent/CN2914092Y/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114137207A (en) * | 2021-11-19 | 2022-03-04 | 佛山墨赛生物技术有限公司 | Test strip, kit and method for quantitative detection of oxycodone |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Leute et al. | Spin immunoassay of opiate narcotics in urine and saliva | |
| CN104749375A (en) | Method for detecting content of C peptide in human serum, kit used by method and preparation method thereof | |
| Munoz et al. | Antigen-Antibody Reactions in Agar: I. Complexity of Antigen-Antibody Systems As Demonstrated by a Serum-Agar Technic | |
| AU592971B2 (en) | Solid phase diffusion assay | |
| CN2914093Y (en) | Colloidal gold test paper for rapidly detecting ketamine | |
| WO1986004683A1 (en) | Determination of clinical parameters by enzyme immunoprocess | |
| Cain et al. | Measurement of angiotensin II in blood by radioimmunoassay | |
| CN2914088Y (en) | Colloidal gold test paper for rapidly detecting phencyclidine | |
| CN2935155Y (en) | Colloidal gold test paper for quick detecting ***e | |
| CN2914091Y (en) | Colloidal gold test paper for rapidly detecting methylenedioxygenmethamphetamine | |
| Mule et al. | Evaluation of the radioimmunoassay for benzoylecgonine (a ***e metabolite) in human urine. | |
| CN102565382A (en) | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples | |
| CN205003159U (en) | Microcystic toxins - LR fluorescence immunity quick quantitative detection card | |
| CN2914092Y (en) | Colloidal gold test paper for rapidly detecting hydroxyldihydrocodeinone | |
| CN203101391U (en) | Gold-labeled immunochromatography detecting system | |
| CN101458252A (en) | Dairy antibiotic rapid detection kit | |
| CN2938101Y (en) | Collidal gold test paper for quickly investigating BZO | |
| CN2938102Y (en) | Collidal gold test paper for quick investigating tricyclic antidepressant | |
| O'Riordan et al. | Immunological reactivity of purified human parathyroid hormone | |
| CN101046479B (en) | Process of preparing human serum base matter containing no target protein | |
| NO781474L (en) | PROCEDURES FOR DETERMINATION OF UNBINDED HORMONS AND PHARMACEUTICALS | |
| CN2914089Y (en) | Colloidal gold test paper for rapidly detecting methadon | |
| CN2935156Y (en) | Colloidal gold test paper for quick detecting bhang | |
| CN103389384A (en) | Miltenberger blood group antibody detection test strip and detection method thereof | |
| CN2914090Y (en) | Colloidal gold test paper for rapidly detecting amphetamine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20070620 |
|
| EXPY | Termination of patent right or utility model |