CN207243877U - A kind of sterile liquid-adding device of bioreactor and its driving device - Google Patents
A kind of sterile liquid-adding device of bioreactor and its driving device Download PDFInfo
- Publication number
- CN207243877U CN207243877U CN201721173160.1U CN201721173160U CN207243877U CN 207243877 U CN207243877 U CN 207243877U CN 201721173160 U CN201721173160 U CN 201721173160U CN 207243877 U CN207243877 U CN 207243877U
- Authority
- CN
- China
- Prior art keywords
- liquid
- branch pipe
- check valve
- bioreactor
- storage bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
A kind of sterile liquid-adding device of bioreactor and its driving device, belong to bio-pharmaceuticals technique research and development equipment, to solve the problems, such as in the zymotechnique development process of bio-pharmaceuticals artificial liquid feeding feed supplement easily pollution and inconvenient.The utility model is made of three-way pipe, syringe and 2 cooling gas check valves, and the branch pipe 1 of wherein three-way pipe can be connected with liquid-adding device, and branch pipe 2 is communicated with external environment, and branch pipe 3 is connected with syringe；Check valve 1 is located on branch pipe 1, and check valve 2 is located on branch pipe 2.The beneficial effects of the utility model are, quantitative liquid feeding feed supplement can be carried out into bioreactor at any time according to the needs in research, syringe, easy-to-use need not be plugged repeatedly, apparatus structure is simple, easy to clean, all components can moist heat sterilization, whole device air-tightness is good, can prevent into bioreactor introduce external source pollution, and it is of low cost, suitable for promote.
Description
Technical field
The utility model belongs to bio-pharmaceuticals technique research and development equipment, and in particular to a kind of sterile liquid feeding dress of bioreactor
Put.
Background technology
Bio-pharmaceuticals is medical industry field 21st century risen and flourished, and main category includes weight
Histone medicine, monoclonal antibody drug etc., at present with CHO（Chinese hamster ovary cell）Based on mammalian cell
The main host cell expressed as medicinal recombinant protein, monoclonal antibody is general using stream plus formula in industrial production（fed-
batch）Training mode carries out zymotechnique in the bioreactor.
In zymotechnique exploitation, in order to obtain the protein expression level of optimization and production cost, it is necessary to biological anti-
Metabolic rule, the nutritional need that expression cell strain is studied on device are answered, establishes cell culture medium and the feed supplement cycle of personalization, therefore
Often it is sampled, tests, and artificial liquid feeding feed supplement is carried out according to test result.Manually into bioreactor, liquid feeding feed supplement is led to
A common syringe by sterilizing carries out liquid feeding by the external interface of bioreactor（Fig. 1）, the shortcomings that this method
In, it is necessary to plug syringe repeatedly, swapping process is readily incorporated pollution, syringe need to tighten after extracting interface prevent gas leakage or
Pollution.
The content of the invention
The problem of for the easy pollution of artificial liquid feeding feed supplement in the zymotechnique development process of bio-pharmaceuticals and inconvenience,
The utility model provides a kind of sterile liquid-adding device of new bioreactor, and its actuating device of atmospheric pressure, and technical solution is such as
Under：
A kind of sterile liquid feeding driving device（Fig. 2）, by three-way pipe（1）, syringe（2）With 2 cooling gas check valves（3、4）Structure
Into the wherein branch pipe 1 of three-way pipe（5）It can be connected with liquid-adding device, branch pipe 2（6）Communicated with external environment, branch pipe 3（7）With injection
Device（2）It is connected；Check valve 1（3）Positioned at branch pipe 1（5）On, only allow gas from threeway tube hub to liquid-adding device one-way flow,
Check valve 2（4）Positioned at branch pipe 2（6）On, only allow gas from external environment to threeway tube hub one-way flow.
The end of the branch pipe 2 can be connected with the filter in 0.22 micron of an aperture（8）.
The center of the three-way pipe be a polypropylene material hard tee pipe fitting, threeway tube hub, check valve 1（3）、
Check valve 2（4）, syringe（2）Or filter（8）Pass through silica gel or rubber between each component and between driving device and liquid-adding device
The hose of material is connected；Check valve 1（3）, check valve 2（4）Valve body material be polypropylene, spool material is silica gel, built-in 304
Stainless steel spring（Fig. 3）.
A kind of sterile liquid-adding device of bioreactor（Fig. 4）, by liquid storage bottle（9）, liquid-feeding tube（10）With above-mentioned sterile liquid feeding
Driving device is formed, wherein liquid storage bottle（9）For the transparent rigid container with volume markings；Liquid-feeding tube（10）One end and liquid storage bottle
（9）Airtight connection and the close liquid storage bottle bottom that is open, the other end can be with bioreactor（11）Filling opening airtight connection；Add
The branch pipe 1 of liquid driving device（3）With liquid storage bottle（9）Airtight connection and the close liquid storage bottle top that is open.
The liquid storage bottle（9）For polyethylene terephthalate material, the liquid-feeding tube（10）For silica gel or rubber
Glue material matter.
The beneficial effects of the utility model are can be carried out quantitatively adding into bioreactor at any time according to the needs in research
Liquid feed supplement, it is not necessary to plug syringe, easy-to-use repeatedly, apparatus structure is simple, easy to clean, and all components damp and hot can go out
Bacterium, whole device air-tightness is good, can prevent into bioreactor introduce external source pollution, and it is of low cost, suitable for promote.
Brief description of the drawings
Fig. 1：Traditional schematic diagram of artificial liquid feeding feed supplement into bioreactor；
Fig. 2：The structure chart of sterile liquid feeding driving device；
Fig. 3：The structure chart of one embodiment of sterile liquid feeding driving device；
Fig. 4：The structure chart of one embodiment of the sterile liquid-adding device of bioreactor；
Fig. 5：The application method schematic diagram of the sterile liquid-adding device of bioreactor.
Reference numeral：
1：Three-way pipe；2：Syringe；3：Check valve 1；4：Check valve 2；5：Branch pipe 1；6：Branch pipe 2；7：Branch pipe 3；8：Filter；
9：Liquid storage bottle；10：Liquid-feeding tube；11：Bioreactor.
Embodiment
Embodiment 1, a kind of sterile liquid feeding driving device
A kind of sterile liquid feeding driving device（Fig. 3）, by three-way pipe（1）, syringe（2）With 2 cooling gas check valves（3、4）Structure
Into the wherein branch pipe 1 of three-way pipe（5）It can be connected with liquid-adding device, branch pipe 2（6）Communicated with external environment, branch pipe 3（7）With injection
Device（2）It is connected；Check valve 1（3）Positioned at branch pipe 1（5）On, only allow gas from threeway tube hub to liquid-adding device one-way flow,
Check valve 2（4）Positioned at branch pipe 2（6）On, only allow gas from external environment to threeway tube hub one-way flow.
The end of the branch pipe 2 is connected with the filter in 0.22 micron of an aperture（8）.
The center of the three-way pipe be a polypropylene material hard tee pipe fitting, threeway tube hub, check valve 1（3）、
Check valve 2（4）, syringe（2）, filter（8）Pass through silica gel material between each component and between driving device and liquid-adding device
Hose is connected；Check valve 1（3）, check valve 2（4）Valve body material be polypropylene, spool material is silica gel, built-in 304 stainless steel
Spring.
Embodiment 2, a kind of sterile liquid feeding driving device
A kind of sterile liquid feeding driving device, by three-way pipe（1）, syringe（2）With 2 cooling gas check valves（3、4）Form, its
The branch pipe 1 of middle three-way pipe（5）It can be connected with liquid-adding device, branch pipe 2（6）Communicated with external environment, branch pipe 3（7）With syringe（2）
It is connected；Check valve 1（3）Positioned at branch pipe 1（5）On, only allow gas from threeway tube hub to liquid-adding device one-way flow, check valve
2（4）Positioned at branch pipe 2（6）On, only allow gas from external environment to threeway tube hub one-way flow.
The center of the three-way pipe be a polypropylene material hard tee pipe fitting, threeway tube hub, check valve 1（3）、
Check valve 2（4）, syringe（2）It is connected between each component and by the hose of rubber material between driving device and liquid-adding device；
Check valve 1（3）, check valve 2（4）Valve body material be polypropylene, spool material is silica gel, built-in 304 stainless steel spring.
Embodiment 3, a kind of sterile liquid-adding device of bioreactor
A kind of sterile liquid-adding device of bioreactor（Fig. 4）, by liquid storage bottle（9）, liquid-feeding tube（10）It is sterile with embodiment 1
Liquid feeding driving device is formed, wherein liquid storage bottle（9）For the transparent rigid container with volume markings；Liquid-feeding tube（10）One end and storage
Liquid bottle（9）Airtight connection and the close liquid storage bottle bottom that is open, the other end can be with bioreactor（11）The closed company of filling opening
Connect；The branch pipe 1 of liquid feeding driving device（3）With liquid storage bottle（9）Airtight connection and the close liquid storage bottle top that is open.
The liquid storage bottle（9）For polyethylene terephthalate material, the liquid-feeding tube（10）For silica gel material.
Embodiment 4, a kind of sterile liquid-adding device of bioreactor
A kind of sterile liquid-adding device of bioreactor, by liquid storage bottle（9）, liquid-feeding tube（10）With the sterile liquid feeding of embodiment 2
Driving device is formed, wherein liquid storage bottle（9）For the transparent rigid container with volume markings；Liquid-feeding tube（10）One end and liquid storage bottle
（9）Airtight connection and the close liquid storage bottle bottom that is open, the other end can be with bioreactor（11）Filling opening airtight connection；Add
The branch pipe 1 of liquid driving device（3）With liquid storage bottle（9）Airtight connection and the close liquid storage bottle top that is open.
The liquid storage bottle（9）For polyethylene terephthalate material, the liquid-feeding tube（10）For rubber material.
Embodiment 5, application method
By the sterile liquid-adding device of bioreactor（Including sterile liquid feeding driving device）Cleaned, moist heat sterilization, drying,
Then the cytotrophy replenisher in research is added in liquid storage bottle, device is assembled, is connect with the outside of bioreactor
Mouth airtight connection（Fig. 5）.When needing liquid feeding, syringe only need to be pushed and pulled repeatedly（2）Push rod, you can pass through pure air produce
Nutritional supplementation liquid in liquid storage bottle is added in bioreactor by air pressure；When liquid feeding is not required, without pulling down device, it can treat whole
Device cleaning is pulled down after the completion of a fermentation process again in case next time uses.
Claims (6)
- A kind of 1. sterile liquid feeding driving device, it is characterised in that it is made of three-way pipe, syringe and 2 cooling gas check valves, wherein The branch pipe 1 of three-way pipe can be connected with liquid-adding device, and branch pipe 2 is communicated with external environment, and branch pipe 3 is connected with syringe；1, check valve In on branch pipe 1, only gas is allowed to be located on branch pipe 2, only allowed from threeway tube hub to liquid-adding device one-way flow, check valve 2 Gas is from external environment to threeway tube hub one-way flow.
- 2. the device described in claim 1, it is characterised in that the filter of the end of the branch pipe 2 and 0.22 micron of an aperture Device is connected.
- 3. the device described in claim 1 or 2, it is characterised in that the center of the three-way pipe is hard for a polypropylene material Matter tee pipe fitting, between threeway tube hub, check valve 1, check valve 2, syringe, each component of filter and liquid feeding driving device is with adding It is connected between liquid device by the hose of silica gel or rubber material.
- 4. the device described in claim 3, it is characterised in that check valve 1, the valve body material of check valve 2 are polypropylene, spool material Matter is silica gel, built-in 304 stainless steel spring.
- 5. a kind of sterile liquid-adding device of bioreactor, it is characterised in that as described in liquid storage bottle, liquid-feeding tube and claim 1 Liquid feeding driving device is formed, and wherein liquid storage bottle is the transparent rigid container with volume markings；Liquid-feeding tube one end and liquid storage bottle are close Close connection and be open close to liquid storage bottle bottom, the other end can be with the charging line airtight connection of bioreactor；Liquid feeding drives The branch pipe 1 of device and liquid storage bottle airtight connection and the close liquid storage bottle top that is open.
- 6. the device described in claim 5, it is characterised in that the liquid storage bottle is polyethylene terephthalate material, The liquid-feeding tube is silica gel or rubber material.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2017201815812 | 2017-02-28 | ||
| CN201720181581 | 2017-02-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207243877U true CN207243877U (en) | 2018-04-17 |
Family
ID=61888573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721173160.1U Active CN207243877U (en) | 2017-02-28 | 2017-09-13 | A kind of sterile liquid-adding device of bioreactor and its driving device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207243877U (en) |
-
2017
- 2017-09-13 CN CN201721173160.1U patent/CN207243877U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206828536U (en) | A kind of coliform detection means | |
| CN202099277U (en) | Simulated rumen in vitro fermentation gas producing device | |
| CN207243877U (en) | A kind of sterile liquid-adding device of bioreactor and its driving device | |
| CN103382440A (en) | Safe inoculation device of liquid fermentation tank | |
| CN102855801B (en) | Recoil infusion apparatus for simulating venous transfusion | |
| CN101480135A (en) | Device for cultivating liquid culture of edible fungus | |
| CN205538940U (en) | Use filter that filter membrane method detected quality of water | |
| CN203999609U (en) | Anerobe dialysis culturing device | |
| CN207031075U (en) | Small test anaerobic organism sewage disposal device | |
| CN212513778U (en) | Sterile sampling device | |
| CN209368236U (en) | A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium | |
| CN203229538U (en) | Feeding, split-charging and collecting device for spinner bottle cultivation of cells | |
| CN101597560A (en) | A kind of device that is used for preparing plating medium | |
| CN205710770U (en) | Integral type T cell culture apparatus | |
| CN206467248U (en) | A kind of cell scale evaluation inlet duct | |
| CN206232714U (en) | A kind of liquid spawn expands numerous device | |
| CN205426558U (en) | Sterility testing sampling device | |
| CN204779580U (en) | Simple and easy artificial rumen blake bottle | |
| CN201284337Y (en) | Culture bottle with ventilating device | |
| CN204325375U (en) | A kind of cell culture fluid adding apparatus | |
| CN212894715U (en) | Aseptic liquid transfer device | |
| CN211645228U (en) | Inflatable plankton sterile culture device of convenient sample | |
| CN205323315U (en) | Aseptic liquid filter of pressure type | |
| CN202152344U (en) | Biological matter fermenting and inoculating device | |
| CN215162550U (en) | Microorganism liquid culture medium subpackaging device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |