CN101597560A - A kind of device that is used for preparing plating medium - Google Patents
A kind of device that is used for preparing plating medium Download PDFInfo
- Publication number
- CN101597560A CN101597560A CN 200910023278 CN200910023278A CN101597560A CN 101597560 A CN101597560 A CN 101597560A CN 200910023278 CN200910023278 CN 200910023278 CN 200910023278 A CN200910023278 A CN 200910023278A CN 101597560 A CN101597560 A CN 101597560A
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- Prior art keywords
- flask
- bottleneck
- plating medium
- preparing
- nutrient solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of device that is used for preparing plating medium, comprise flask, fill the nutrient solution that is in temperature constant state for preparing in advance in the flask, be provided with fairlead to bottleneck in the described flask, it is characterized in that, described bottleneck is provided with sealing plug, fairlead is by the aspirator of sealing plug UNICOM band negative pressure interface to bottleneck, the outlet of aspirator is guided the quantitative perfusion device that the position that is arranged on the worktable is lower than flask into by a flexible pipe, constant temperature culture liquid in the described flask is added in this quantitative perfusion device through flexible pipe by the syphonic effect of aspirator, is diverted to the substratum that forms different diameter and thickness in the required culture dish by this quantitative perfusion device.
Description
Technical field
The present invention relates to a kind of preparation facilities that is used for microbiological test to various substratum, particularly a kind of device of blood agar medium preparation.
Background technology
In recent years, along with constantly novel epiphytotics appearance, the research of pathogenic micro-organism and virus is subjected to the concern and the attention of medical circle so, so the preparation of substratum is just particularly important.In addition, hundreds and thousands of (propping up) various substratum of the also seasonal needs of student experimenting of Experiment on Microbiology teaching class.It is too big and long-time idle to buy the required investment of large-scale automatic separated loading instrument.When adopting prior art preparation substratum, the flat board of pouring out (test tube) is uneven, and can not be quantitative, directly influences experiment effect.
Summary of the invention
The present invention is directed to the existing problem of background technology, provide a kind of can be middle-size and small-size scientific research, educational institution's science, succinct, economical, easily and fast, and can reach the experimental installation that is used for the plate culture medium preparation of aseptic result.
For reaching above purpose, the present invention takes following technical scheme to be achieved:
A kind of device that is used for preparing plating medium, comprise flask, fill the nutrient solution that is in temperature constant state for preparing in advance in the flask, be provided with fairlead to bottleneck in the described flask, it is characterized in that, described bottleneck is provided with sealing plug, fairlead is by the aspirator of sealing plug UNICOM band negative pressure interface to bottleneck, the outlet of aspirator is guided the quantitative perfusion device that the position that is arranged on the worktable is lower than flask into by a flexible pipe, constant temperature culture liquid in the described flask is added in this quantitative perfusion device through flexible pipe by the syphonic effect of aspirator, is diverted to the substratum that forms different diameter and thickness in the required culture dish by this quantitative perfusion device.
In the such scheme, described quantitative perfusion device comprises the cover frame of an open front, this cover frame is provided with a plurality of test tubes that row's capacity does not wait, and the lower end of each test tube is equipped with control valve, with convenient control to nutrient solution perfusion capacity in the culture dish on its below worktable.Described a plurality of test tube is three, and its capacity is respectively 5ml, 20ml, 30ml.
Described flask shoulder is provided with a mouth that heals that is used to make the blood agar substratum.The bottle of described flask is provided with the magnetic blade rotor in the end, by at the bottom of the bottle outside, be provided with a magnetic stirring apparatus substratum in the bottle is carried out the timing stirring.
In the described flask, the air chamber above nutrient solution is provided with another pipe by the unidirectional air bag UNICOM outside sealing plug and the bottleneck, can be used for that nutrient solution in the flask is executed malleation and draws.
The present invention can be adapted to the making of various substratum, and can hold many people simultaneously uses, having overcome the substratum that in the past contains agar solidifies easily, and quantitatively property and inaesthetic defective, have no time limit and homothermic advantage, the making to the blood agar substratum has more superiority especially.
Description of drawings
Fig. 1 is the structural representation of device of the present invention.
Among the figure: 1, Bechtop; 2, magnetic stirring apparatus; 3, magnetic blade rotor; 4, flask; 5, unidirectional air bag; 6, fairlead; 7, negative pressure interface; 8, heal mouth; 9, nutrient solution; 10, flexible pipe; 11, flexible pipe outlet; 12, quantitative test tube; 13, quantitative filling cover frame; 14, culture dish; 15, sucker; 16, control valve.
Embodiment
The present invention is described in further detail below in conjunction with drawings and the specific embodiments.
As shown in Figure 1, a kind of device that is used for preparing plating medium, comprise flask 4, fill the nutrient solution that is in temperature constant state 9 for preparing in advance in the flask, be provided with glass fairlead 6 in the flask to bottleneck, bottleneck is provided with sealing plug, and fairlead 6 is by meet at right angles to the bottleneck aspirator of one of UNICOM band negative pressure interface 7 of sealing plug, and the outlet of aspirator is guided the quantitative perfusion device that the position that is arranged on the worktable 1 is lower than flask 4 into by a flexible pipe 10 (medical sebific duct).The negative pressure interface 7 of aspirator can directly add negative pressure with 60 milliliters of syringes flows out nutrient solution; The outlet 11 of flexible pipe 10 is provided with the ball valve of a may command sucking-off nutrient solution flow.Quantitatively perfusion device comprises the cover frame 13 of an open front, and cover frame below respectively has a sucker 15 will cover frame for four jiaos to be fixed on the Bechtop 1; This cover frame is provided with row's capacity and is respectively three test tubes 12 that 5ml, 20ml, 30ml do not wait, each test tube upper end open, connect the nutrient solution of outlet 11 sucking-offs of flexible pipe 10, the lower end is equipped with control valve 16, with convenient control to nutrient solution perfusion capacity in the culture dish 14 on its below worktable.
The flask shoulder be provided with one be used to make the blood agar substratum heal mouthfuls 8, blood quantitatively can be added in the nutrient solution 9.The bottle of flask is provided with magnetic blade rotor 3 in the end, by at the bottom of the bottle outside, be provided with a magnetic stirring apparatus 2 can be to nutrient solution in the bottle, particularly the blood agar nutrient solution carries out the timing stirring, in case solidify.An end band certain camber of fairlead 6 at the bottom of the bottle is in case the influence that spline 3 stirs.
As a kind of selection scheme, in the flask of the above embodiment of the present invention, also can be provided with another pipe by unidirectional air bag 5 UNICOMs outside sealing plug and the bottleneck by the air chamber above nutrient solution, can be used for executing malleation and drawing to nutrient solution in the flask is auxiliary.
Claims (6)
1, a kind of device that is used for preparing plating medium, comprise flask, fill the nutrient solution that is in temperature constant state for preparing in advance in the flask, be provided with fairlead to bottleneck in the described flask, it is characterized in that, described bottleneck is provided with sealing plug, fairlead is by the aspirator of sealing plug UNICOM band negative pressure interface to bottleneck, the outlet of aspirator is guided the quantitative perfusion device that the position that is arranged on the worktable is lower than flask into by a flexible pipe, constant temperature culture liquid in the described flask is added in this quantitative perfusion device through flexible pipe by the syphonic effect of aspirator, is diverted to the substratum that forms different diameter and thickness in the required culture dish by this quantitative perfusion device.
2, the device that is used for preparing plating medium as claimed in claim 1, it is characterized in that, described quantitative perfusion device comprises the cover frame of an open front, this cover frame is provided with a plurality of test tubes that row's capacity does not wait, the lower end of each test tube is equipped with control valve, with convenient control to nutrient solution perfusion capacity in the culture dish on its below worktable.
3, the device that is used for preparing plating medium as claimed in claim 2 is characterized in that, described a plurality of test tubes are three, and its capacity is respectively 5ml, 20ml, 30ml.
4, the device that is used for preparing plating medium as claimed in claim 1 is characterized in that, described flask shoulder is provided with a mouth that heals that is used to make the blood agar substratum.
5, the device that is used for preparing plating medium as claimed in claim 1 is characterized in that, the bottle of described flask is provided with the magnetic blade rotor in the end, by at the bottom of the bottle outside, be provided with a magnetic stirring apparatus substratum in the bottle is carried out the timing stirring.
6, the device that is used for preparing plating medium as claimed in claim 1, it is characterized in that, in the described flask, the air chamber above nutrient solution is provided with another pipe by the unidirectional air bag UNICOM outside sealing plug and the bottleneck, is used for that the flask nutrient solution is executed malleation and draws.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910023278 CN101597560B (en) | 2009-07-10 | 2009-07-10 | Device for preparing plating medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910023278 CN101597560B (en) | 2009-07-10 | 2009-07-10 | Device for preparing plating medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101597560A true CN101597560A (en) | 2009-12-09 |
| CN101597560B CN101597560B (en) | 2011-11-16 |
Family
ID=41419159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910023278 Expired - Fee Related CN101597560B (en) | 2009-07-10 | 2009-07-10 | Device for preparing plating medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101597560B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226145A (en) * | 2011-04-09 | 2011-10-26 | 河南科技学院 | Culture medium preparation device and method |
| CN104118836A (en) * | 2014-07-29 | 2014-10-29 | 山东师范大学 | Device for filling culture medium under aseptic condition and aseptic filling method |
| CN112481123A (en) * | 2020-11-16 | 2021-03-12 | 大连理工大学 | Microfluidic system and method for researching shear force and biochemical factor gradient regulation and control of cell scratch repair |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2135078Y (en) * | 1992-08-13 | 1993-06-02 | 孙廷山 | Multi-functional liquid extractor |
| CN2287149Y (en) * | 1996-05-24 | 1998-08-05 | 李敬波 | Automatic liquid sucker |
| CN201027167Y (en) * | 2007-04-11 | 2008-02-27 | 宁波大学 | Split charging device for simple culture medium |
-
2009
- 2009-07-10 CN CN 200910023278 patent/CN101597560B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226145A (en) * | 2011-04-09 | 2011-10-26 | 河南科技学院 | Culture medium preparation device and method |
| CN104118836A (en) * | 2014-07-29 | 2014-10-29 | 山东师范大学 | Device for filling culture medium under aseptic condition and aseptic filling method |
| CN104118836B (en) * | 2014-07-29 | 2015-07-29 | 山东师范大学 | The filling device of culture and aseptic filling method is carried out under a kind of aseptic condition |
| CN112481123A (en) * | 2020-11-16 | 2021-03-12 | 大连理工大学 | Microfluidic system and method for researching shear force and biochemical factor gradient regulation and control of cell scratch repair |
| CN112481123B (en) * | 2020-11-16 | 2022-02-15 | 大连理工大学 | Microfluidic system and method for researching shear force and biochemical factor gradient regulation and control of cell scratch repair |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101597560B (en) | 2011-11-16 |
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Granted publication date: 20111116 Termination date: 20120710 |