CN103382440A - Safe inoculation device of liquid fermentation tank - Google Patents
Safe inoculation device of liquid fermentation tank Download PDFInfo
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- CN103382440A CN103382440A CN2013102508254A CN201310250825A CN103382440A CN 103382440 A CN103382440 A CN 103382440A CN 2013102508254 A CN2013102508254 A CN 2013102508254A CN 201310250825 A CN201310250825 A CN 201310250825A CN 103382440 A CN103382440 A CN 103382440A
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- inoculation
- sterile air
- fermentation tank
- pipe
- bottle
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Abstract
The invention relates to a component of biological fermentation equipment, and in particular to an inoculation device of a liquid fermentation tank case. The device comprises an inoculation bottle, and a bottle cap of the inoculation device is hermetically connected with a sterile filtration respirator and an inoculation pipe with a diaphragm valve; the inoculation pipe is connected with an inlet of a sterile air intake pipe of a fermentation tank through a middle pipeline, and the inlet of the sterile air intake pipe is provided with a branch pipe connected with a sterile air source; the fermentation tank is also provided with a sterile air outtake pipe. The device has simple structure and convenient operation; with arrangement of the inoculation bottle and the fermentation tank which have one-way flow bacteria, the device reduces the chance of the fermentation tank internal environment contacting external air, and thereby avoiding bacteria pollution and improving the success rate of inoculation.
Description
Technical field
The present invention relates to kind of biological fermentation equipment parts, specifically a kind of liquid fermentation tank case classification inoculation apparatus.
Background technology
Liquid fermentation tank needs first fermentor tank to be carried out disinfecting action when carrying out biological fermentation process, then inoculates under aseptic, reliably carries out with the fermenting process that guarantees the target bacterial classification.Existing liquid fermentation tank is difficult to avoid in seeded process and the contacting of extraneous air, and easily causes miscellaneous bacteria to sneak into, and makes the fermenting process failure.
Summary of the invention
Technical problem to be solved by this invention is that a kind of simple in structure, easy to operate, safe classification inoculation apparatus of liquid fermentation tank that success ratio of inoculation is high is provided.
The safe classification inoculation apparatus of liquid fermentation tank of the present invention includes an inoculation bottle, is sealedly connected with inoculated tube and sterile filtration respiratory organ with diaphragm valve on the bottle cap of inoculation bottle; Inoculated tube is connected by the entrance of the sterile air inlet pipe on one section intermediate duct and a fermentor tank, and the entrance of described sterile air inlet pipe also has one tunnel branch to be connected with source of sterile air; Also be provided with a sterile air escape pipe on fermentor tank.
Be connected with respectively steam inlet duct and steam outleting pipe before and after described intermediate duct, be used for middle pipeline is carried out disinfecting action.
Described source of sterile air enters in the branch of sterile air inlet pipe and is provided with valve, also is provided with valve on the sterile air escape pipe.
The present invention is simple in structure, easy to operate, and its inoculation bottle and fermentor tank by the bacterial classification uniflux is set has reduced the touch opportunity of environment and extraneous air in fermentor tank, has avoided living contaminants, has improved the success ratio of inoculating.
Description of drawings
Fig. 1 is structural representation of the present invention.
Embodiment
As shown in the figure, the safe classification inoculation apparatus of this liquid fermentation tank includes an inoculation bottle 1, is sealedly connected with inoculated tube 2 and sterile filtration respiratory organ 3 with diaphragm valve on the bottle cap of inoculation bottle; Inoculated tube is connected by the entrance of the sterile air inlet pipe 6 on one section intermediate duct 4 and a fermentor tank 5, and the entrance of described sterile air inlet pipe also has one tunnel branch 7 to be connected with source of sterile air; Also be provided with a sterile air escape pipe 8 on fermentor tank.Described intermediate duct 4 front and back are connected with respectively steam inlet duct 9 and steam outleting pipe 10, are used for middle pipeline is carried out disinfecting action.Described source of sterile air enters in the branch 7 of sterile air inlet pipe and is provided with valve, also is provided with valve on sterile air escape pipe 8.
The working process of this device is:
1, cultured bacterial classification is poured in the inoculation bottle of sterilising treatment in super quiet worktable, closed inoculation bottle;
2, inoculated tube is connected with the sterile air inlet pipe of fermentor tank;
3, pass into steam and carry out the pipeline sterilization;
4, open valve and pass into sterile air.
During inoculation owing to passing into sterile air, make under the effect of air-flow in inoculation pipeline and inoculation bottle and produce negative pressure, because suction function is brought the bacterial classification in inoculation bottle in fermentor tank into, complete the safety access of bacterial classification, close respective valves after finished to unload classification inoculation apparatus.
Claims (3)
1. safe classification inoculation apparatus of liquid fermentation tank, it is characterized in that: it includes an inoculation bottle (1), is sealedly connected with inoculated tube (2) and sterile filtration respiratory organ (3) with diaphragm valve on the bottle cap of inoculation bottle; Inoculated tube is connected by the entrance of the sterile air inlet pipe (6) on one section intermediate duct (4) and a fermentor tank (5), and the entrance of described sterile air inlet pipe also has one tunnel branch (7) to be connected with source of sterile air; Also be provided with a sterile air escape pipe (8) on fermentor tank.
2. the safe classification inoculation apparatus of liquid fermentation tank according to claim 1, is characterized in that: be connected with respectively steam inlet duct (9) and steam outleting pipe (10) before and after described intermediate duct (4).
3. the safe classification inoculation apparatus of liquid fermentation tank according to claim 1 is characterized in that: described source of sterile air enters in the branch (7) of sterile air inlet pipe and is provided with valve, and the sterile air escape pipe also is provided with valve on (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102508254A CN103382440A (en) | 2013-06-24 | 2013-06-24 | Safe inoculation device of liquid fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102508254A CN103382440A (en) | 2013-06-24 | 2013-06-24 | Safe inoculation device of liquid fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103382440A true CN103382440A (en) | 2013-11-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102508254A Pending CN103382440A (en) | 2013-06-24 | 2013-06-24 | Safe inoculation device of liquid fermentation tank |
Country Status (1)
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| CN (1) | CN103382440A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142199A (en) * | 2017-06-06 | 2017-09-08 | 温州市伯泰机械科技有限公司 | Automatic koji machine |
| CN107723225A (en) * | 2017-11-14 | 2018-02-23 | 广州普麟生物制品有限公司 | A kind of classification inoculation apparatus and inoculation method |
| CN107988056A (en) * | 2017-11-24 | 2018-05-04 | 成都凯成轻工药业装备有限公司 | A kind of strain scene classification inoculation apparatus and method |
| CN108949517A (en) * | 2017-05-19 | 2018-12-07 | 上海吉态来生物技术有限公司 | A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe |
| CN112680350A (en) * | 2021-01-03 | 2021-04-20 | 付雪斌 | Air interchanger for cell culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202022936U (en) * | 2011-03-24 | 2011-11-02 | 牡丹江佰佳信生物科技有限公司 | Sealed fermentation tank inoculating system |
| CN102511315A (en) * | 2012-01-07 | 2012-06-27 | 李好勇 | Liquid edible fungus strain culture device and culture method |
| CN103039269A (en) * | 2012-12-24 | 2013-04-17 | 何寒 | Simple liquid strain inoculation device |
-
2013
- 2013-06-24 CN CN2013102508254A patent/CN103382440A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202022936U (en) * | 2011-03-24 | 2011-11-02 | 牡丹江佰佳信生物科技有限公司 | Sealed fermentation tank inoculating system |
| CN102511315A (en) * | 2012-01-07 | 2012-06-27 | 李好勇 | Liquid edible fungus strain culture device and culture method |
| CN103039269A (en) * | 2012-12-24 | 2013-04-17 | 何寒 | Simple liquid strain inoculation device |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949517A (en) * | 2017-05-19 | 2018-12-07 | 上海吉态来生物技术有限公司 | A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe |
| CN107142199A (en) * | 2017-06-06 | 2017-09-08 | 温州市伯泰机械科技有限公司 | Automatic koji machine |
| CN107723225A (en) * | 2017-11-14 | 2018-02-23 | 广州普麟生物制品有限公司 | A kind of classification inoculation apparatus and inoculation method |
| CN107988056A (en) * | 2017-11-24 | 2018-05-04 | 成都凯成轻工药业装备有限公司 | A kind of strain scene classification inoculation apparatus and method |
| CN112680350A (en) * | 2021-01-03 | 2021-04-20 | 付雪斌 | Air interchanger for cell culture |
| CN112680350B (en) * | 2021-01-03 | 2022-11-08 | 中科细胞再生康复医疗(山东)有限公司 | Air interchanger for cell culture |
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Application publication date: 20131106 |