CN211645228U - Inflatable plankton sterile culture device of convenient sample - Google Patents
Inflatable plankton sterile culture device of convenient sample Download PDFInfo
- Publication number
- CN211645228U CN211645228U CN201922311977.6U CN201922311977U CN211645228U CN 211645228 U CN211645228 U CN 211645228U CN 201922311977 U CN201922311977 U CN 201922311977U CN 211645228 U CN211645228 U CN 211645228U
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- Prior art keywords
- sampling
- culture
- plankton
- pipe
- valve
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- 238000005070 sampling Methods 0.000 claims abstract description 70
- 238000000034 method Methods 0.000 abstract description 9
- 239000000463 material Substances 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 29
- 230000007797 corrosion Effects 0.000 description 3
- 238000005260 corrosion Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- -1 polytetrafluoroethylene Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses aseptic culture apparatus of inflatable plankton of convenient sample, include: a culture bottle; one end of the sampling tube penetrates into the culture bottle from the bottom of the culture bottle upwards; the sampling valve is connected to the other end of the sampling pipe; one end of the air inlet pipe penetrates into the culture bottle from the bottom of the culture bottle upwards; the sampling valve is connected with the air inlet valve; and the air source is connected with the air inlet valve through the air feed pipe. The utility model has the advantages of it is following: the sampling is more convenient; the method of bridging the sampling pipe and the air inlet pipe by the three-way valve solves the problem that the residual pipe of the sample liquid after sampling influences the next sampling; an inverted culture bottle is adopted to form an inverted triangle at the bottom, and air is fed in a matching manner, so that the culture system is mixed more uniformly and fully, and the sampling precision is improved; the material that can disinfect is selected to other external air entering culture system are isolated in cultivation, the sample process, and the aseptic environment of better assurance experimentation.
Description
Technical Field
The utility model relates to an ocean and aquatic organisms science field, a portable sample's aseptic culture apparatus of inflatable plankton specifically says so.
Background
Although the conventional common plankton culture device can ensure that gas passes through a gas sterile filter when entering a culture system, the problems that sterility cannot be ensured in the sampling process, the sampling is left in a sampling pipe after the sampling is finished, the next sampling is influenced and the like exist.
In the prior art, Chinese patent 'a planktonic microorganism culture thermostat' (publication number: 208717348U) discloses a planktonic microorganism culture thermostat, which comprises a rectangular box body, a pair of rollers are arranged in the rectangular box body, a diversion belt is sleeved on the pair of rollers, a plurality of rectangular plates are uniformly arranged on the diversion belt, the supporting legs are connected to the inner lower surface of the rectangular box body, the surface of one side of the rectangular box body is connected with a connecting plate, a motor is arranged on the connecting plate, the output end of the motor penetrates through the rectangular box body and is connected with a belt between the rollers, one end of the water inlet pipe is connected to the water pump, an air compression pump is arranged on the surface of one side of the box body, the air compression pump output end is connected with the trachea, evenly open the gas pocket that has a plurality of on the trachea, a side surface is equipped with the LED lamp in the rectangle box. The utility model discloses simple structure, convenient operation has reduced user's labour, and higher emulation goes out the ecological environment of plankton, can increase oxygen at any time when the water oxygen deficiency, has improved the survival rate of plankton.
These problems of the prior art increase data errors in the cultivation and study of plankton including algae and zooplankton, and affect experimental results.
SUMMERY OF THE UTILITY MODEL
To the defect among the prior art, the utility model provides a solve the aseptic culture apparatus of inflatable plankton of the convenient sample of above-mentioned technical problem.
In order to solve the technical problem, the utility model discloses inflatable plankton sterile culture device of convenient sample, include: a culture bottle;
a sampling tube, wherein one end of the sampling tube penetrates into the culture bottle from the bottom of the culture bottle upwards;
the sampling valve is connected to the other end of the sampling pipe;
one end of the air inlet pipe penetrates into the culture bottle from the bottom of the culture bottle upwards;
the sampling valve is connected with the air inlet valve;
and the air source is connected with the air inlet valve through an air feed pipe.
Preferably, the sampling valve and the intake valve are three-way valves.
Preferably, the sampling valve is connected with the air inlet valve through a bridging pipe.
Preferably, a supply gas aseptic filter is provided on the supply pipe.
Preferably, a gas check valve is arranged on the gas feeding pipe.
Preferably, the culture bottle further comprises an exhaust pipe, and one end of the exhaust pipe penetrates into the culture bottle from the bottom of the culture bottle upwards.
Preferably, an exhaust gas aseptic filter is provided at the other end of the exhaust pipe.
Preferably, a bottle cap is arranged at the bottom of the culture bottle, and one end of the sampling tube, the air inlet tube and the exhaust tube penetrates into the culture bottle through the bottle cap.
Preferably, the flask is fixed upside down on a tripod.
Compared with the prior art, the utility model discloses the aseptic culture apparatus of inflatable plankton of convenient sample has following advantage:
1) the utility model has the characteristics of more convenient sampling;
2) the utility model adopts a mode that a three-way valve is bridged with a sampling pipe and an air inlet pipe, and solves the problem that the residual pipe of the sample liquid influences the next sampling after the sampling;
3) the utility model adopts the inverted culture bottle to form the bottom inverted triangle, and matches with the air inlet, so that the culture system is mixed more uniformly and fully, and the sampling precision is improved;
4) the device of the utility model selects the sterilizable material, and isolates other external gases from entering the culture system in the culture and sampling processes, thereby better ensuring the sterile environment in the experimental process;
5) all parts of the device are low in cost, easy to obtain and strong in popularization;
6) the utility model discloses the device can be applied to cultivateing such as microorganism, alga, zooplankton, utensil application universality.
Drawings
Other features and advantages of the present invention will become more apparent upon reading the following detailed description of non-limiting embodiments thereof, with reference to the accompanying drawings.
FIG. 1 is a schematic structural view of the inflatable plankton sterile culture device for convenient sampling of the present invention;
FIG. 2 is a schematic view of the gas direction of the inflatable plankton sterile culture device for convenient sampling according to the present invention;
FIG. 3 is a schematic view of the gas flow direction after the sampling of the inflatable plankton sterile culture device for convenient sampling of the present invention is finished;
fig. 4 is a schematic view of the gas direction of the inflatable plankton sterile culture device for convenient sampling of the utility model in normal operation.
In the figure:
1-culture bottle 2-bottle cap 3-tripod
4-sampling pipe 5-sampling valve 6-bridging pipe
7-exhaust pipe 8-inlet pipe 9-exhaust gas sterile filter
10-air inlet valve 11-gas one-way valve 12-gas supply gas sterile filter
13-air supply pipe
Detailed Description
The present invention will be described in detail with reference to the following embodiments. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that various changes and modifications can be made by one skilled in the art without departing from the spirit of the invention. These all belong to the protection scope of the present invention.
Although the conventional common plankton culture device can ensure that gas passes through a gas sterile filter when entering a culture system, the problems that sterility cannot be ensured in the sampling process, the sampling is left in a sampling pipe after the sampling is finished, the next sampling is influenced and the like exist. These problems can add data errors to the development and study of plankton including algae and zooplankton, and affect experimental results.
As shown in fig. 1 to 4, the inflatable type plankton sterile culture device for convenient sampling of the present invention is composed of a main culture bottle 1, a three-way bottle cap 2, a tripod 3, a sampling tube 4, a three-way sampling valve 5, a three-way bridging tube 6, an exhaust pipe 7, an intake pipe 8, an exhaust gas sterile filter 9, a three-way intake valve 10, a gas one-way valve 11, an air supply gas sterile filter 12, and an air supply pipe 13.
The three-way sampling valve 5 of the device has A, B, C three channels, the three-way air inlet valve 10 has D, E, F three channels, and the silica gel hose is in bridge connection with C, D two channels.
In the device, a culture bottle 1 is inversely fixed on a tripod 3 and is matched with the air inlet position setting of an air inlet pipe 8, so that the culture system is more fully mixed in the aeration process. The gas is filtered of bacteria and other contaminants by the feed gas aseptic filter 12; then enters the device body through the gas one-way valve 11, and the gas one-way valve 11 can prevent liquid from entering the gas-feeding gas sterile filter 12 and the gas-feeding pipe 13 along the gas-feeding pipe.
During the daily culture process, the B, C channel of the three-way sampling valve 5 is opened, and the E, F channel of the three-way air inlet valve 10 is opened; the gas passes only through the inlet pipe 8.
During sampling, the A, B, C channel of the three-way sampling valve 5 is opened, and the sample is taken out from the channel A. The D, E, F passage of the three-way intake valve 10 is open; the gas is divided into 2 paths, and the 1 path enters the culture system through a channel F and an air inlet pipe 8, so that the samples are fully mixed, and the sampling precision is ensured; the other 1 path is discharged out of the system along with the sample through a channel D, a bridging pipe 6, a channel C and a channel A, so that the sample cannot remain in the sampling pipe, and external gas is isolated from entering the system.
After sampling, the B, C channel of the three-way sampling valve 5 is opened, the D, E channel of the three-way air inlet valve 9 is opened and the F channel is closed at the same time, and the D channel is closed after all residual samples in the sampling pipe return to the culture bottle, so that the daily culture process is started.
In order to realize the whole wet heat sterilization of the device main body. The culture bottle 1 in the device is made of high borosilicate material; the three-way bottle cap 2 is made of corrosion-resistant and high-temperature polytetrafluoroethylene material; the sampling tube 4, the air inlet pipe 8 and the exhaust pipe 7 are made of corrosion-resistant and high-temperature Teflon hard tubes. The three-way sampling valve 5, the three-way air inlet valve 10 and the gas one-way valve 11 are made of PP materials with corrosion resistance and high temperature. The connecting pipe between the device components uses a food-grade high-temperature-resistant and high-pressure-resistant transparent silica gel hose.
The utility model discloses fine solution above problem. The device adopts the cost-controllable materials such as glass bottles, PTFE three-way bottle caps, silicone tubes, Teflon hard tubes, polypropylene three-way valves and the like which can be sterilized at high temperature and high pressure, and greatly improves the realizability, the practicability and the simplicity of the system through convenient connection. The utility model can be widely applied to the experiments of microbial fermentation, microalgae culture, macroalgae seedling culture and partial micro zooplankton incubation culture.
The foregoing description of the specific embodiments of the invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (9)
1. The utility model provides an aseptic culture apparatus of inflatable plankton of convenient sample which characterized in that includes:
a culture bottle;
a sampling tube, wherein one end of the sampling tube penetrates into the culture bottle from the bottom of the culture bottle upwards;
the sampling valve is connected to the other end of the sampling pipe;
one end of the air inlet pipe penetrates into the culture bottle from the bottom of the culture bottle upwards;
the sampling valve is connected with the air inlet valve;
and the air source is connected with the air inlet valve through an air feed pipe.
2. The portable sampling, aerated plankton acclimatization device according to claim 1, wherein the sampling valve and the air intake valve are three-way valves.
3. The portable sampling, aerated plankton acclimatization device according to claim 2, wherein the sampling valve is connected with the air inlet valve through a bridge pipe.
4. The portable sampling inflatable plankton sterile culture device of claim 3, wherein an air supply gas sterile filter is provided on the air supply pipe.
5. The portable sampling inflatable plankton sterile culture device of claim 4, wherein a gas check valve is arranged on the gas supply pipe.
6. The portable sampling inflatable plankton sterile culture device of claim 5, further comprising an exhaust pipe, wherein one end of the exhaust pipe penetrates into the culture bottle from the bottom of the culture bottle upwards.
7. The portable sampling inflatable plankton sterile culture device of claim 6, wherein an exhaust gas sterile filter is arranged at the other end of the exhaust pipe.
8. The portable sampling inflatable plankton sterile culture device of claim 7, wherein a bottle cap is arranged at the bottom of the culture bottle, and one end of the sampling tube, the air inlet tube and the air exhaust tube is inserted into the culture bottle through the bottle cap.
9. The portable sampling, aerated plankton culture device of claim 8, wherein said culture flask is mounted upside down on a tripod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922311977.6U CN211645228U (en) | 2019-12-20 | 2019-12-20 | Inflatable plankton sterile culture device of convenient sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922311977.6U CN211645228U (en) | 2019-12-20 | 2019-12-20 | Inflatable plankton sterile culture device of convenient sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211645228U true CN211645228U (en) | 2020-10-09 |
Family
ID=72697601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922311977.6U Expired - Fee Related CN211645228U (en) | 2019-12-20 | 2019-12-20 | Inflatable plankton sterile culture device of convenient sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211645228U (en) |
-
2019
- 2019-12-20 CN CN201922311977.6U patent/CN211645228U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201009 |
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| CF01 | Termination of patent right due to non-payment of annual fee |