CN203878151U - Sample extractor - Google Patents
Sample extractor Download PDFInfo
- Publication number
- CN203878151U CN203878151U CN201420160894.6U CN201420160894U CN203878151U CN 203878151 U CN203878151 U CN 203878151U CN 201420160894 U CN201420160894 U CN 201420160894U CN 203878151 U CN203878151 U CN 203878151U
- Authority
- CN
- China
- Prior art keywords
- magnetic
- separation vessel
- magnet base
- magnetic frame
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000005291 magnetic effect Effects 0.000 claims abstract description 99
- 238000000926 separation method Methods 0.000 claims abstract description 53
- 239000012634 fragment Substances 0.000 claims description 15
- 238000000605 extraction Methods 0.000 claims description 9
- 239000000523 sample Substances 0.000 abstract description 13
- 239000012472 biological sample Substances 0.000 abstract description 5
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 18
- 239000007788 liquid Substances 0.000 description 11
- 239000011324 bead Substances 0.000 description 6
- 239000003292 glue Substances 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000012445 acidic reagent Substances 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 229910052779 Neodymium Inorganic materials 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- ZGDWHDKHJKZZIQ-UHFFFAOYSA-N cobalt nickel Chemical compound [Co].[Ni].[Ni].[Ni] ZGDWHDKHJKZZIQ-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a sample extractor which comprises a magnetic rack, wherein the magnetic rack comprises a bracket and a magnet base arranged on the bracket; magnetic parts are installed in the magnet base; the bracket is provided with a locking device; the locking device comprises elastic pieces and elastic piece brackets arranged on both ends of the magnetic rack; and the each elastic piece is installed on the corresponding elastic piece bracket. A separation vessel filled with a biological sample can be locked to the magnetic rack by the locking device when being arranged on the magnet base. The arrangement and fixation of the separation vessel are simultaneously completed in one step without any additional fixation step, thereby enhancing the separation efficiency of the biological sample.
Description
Technical field
The utility model relates to biological specimen separation and Extraction field, relates in particular to a kind of device that utilizes magnetic force to extract sample.
Background technology
Magnetic separation technique has a wide range of applications in the separating of cytobiology, immunology, diagnostics and pharmacy etc., and can realize the object of the materials such as fast separating and purifying cell, albumen or nucleic acid.As compared with the precipitator method, centrifuging, ion exchange column method, magnetic separation technique has that separating device is few, velocity of separation fast, extraction efficiency high with conventional partition method.
Magnetic frame is adsorbable magnetic particle in biological magnetic lock out operation, thereby realizes sharp separation and the purifying of target molecule in complex mixture, is a kind of simple in structure, easy to operate magnetic separating apparatus.When use, will the separation vessel of biological sample be housed, for example 96 orifice plates, put into magnetic frame.Magnetic substance in sample will be enriched on the magneticsubstance in separation agent, and is fitted on the tube wall of separation vessel.Remove not by the material of magnetic absorption, and through obtaining the biological sample of purifying after repeatable operation repeatedly.But existing magnetic frame is only used for support separation vessel, the separation vessel of putting thereon can not be fixed.In extraction from biological material process, if upset separation vessel, existing magnetic frame just can not complete.Because in the time that magnetic frame overturns, separation vessel can drop from magnetic frame, so existing magnetic frame can not adapt to more lock out operation.
Utility model content
For overcoming the deficiencies in the prior art, the purpose of this utility model is to provide a kind of sample extraction device, comprise magnetic frame, described magnetic frame comprises support, be placed on the magnet base on support, magnetic part is installed in magnet base, locking latches has been installed on support, described locking latches comprises the shell fragment and the shell fragment support that are arranged on magnetic frame two ends, and shell fragment is arranged on shell fragment support.
Further, described shell fragment comprises elastic arm and shell foot.
Further, the angle between elastic arm and shell foot is greater than 90 degree.
Further, the shell foot bottom surface of described shell foot does not touch the upper surface of magnet base.
Further, the distance between shell foot bottom surface and magnet base is just in time the height of separation vessel.
Further, on magnet base, also comprise support hole.
The beneficial effects of the utility model are: magnetic frame described in the utility model can be fixed the separation vessel of putting thereon, in biological specimen leaching process, if upset separation vessel, separation vessel just can not drop from magnetic frame, and what therefore biological specimen extracted operates easily.Locking latches described in the utility model makes the placement of 96 orifice plates and is fixed in a step to complete simultaneously, and does not need to increase extra fixing step.In the time that magnetic part adopts convex shape structure, the combination between magnetic part and magnet base just does not need to fix with glue.In the time that magnetic part is impaired, can completes more and change jobs easily like this.Design described in the utility model has increased the contact area of magnet and separation vessel, increases the area of magnetic absorption, has improved the separation efficiency of biological specimen.
Brief description of the drawings
Fig. 1 is magnetic frame structural representation.
Fig. 2 is the magnetic frame structural representation that separation vessel is housed.
Fig. 3 is the first location schematic diagram of separation vessel at elastic piece structure locking latches.
Fig. 4 is the second position schematic diagram of separation vessel at elastic piece structure locking latches.
Fig. 5 is three position view of separation vessel at elastic piece structure locking latches.
Fig. 6 is convex shape magnetic part holding tank structural representation.
Fig. 7 is the magnetic part holding tank structural representation of tool ramp structure.
Embodiment
Below in conjunction with concrete accompanying drawing, the utility model is described in detail.These specific embodiments are only limited the enumerating under the utility model spirit, do not get rid of one of ordinary skill in the art prior art and the utility model in conjunction with and other specific embodiments of producing.
As illustrated in fig. 1 and 2, magnetic frame 100 for separating of biological specimen comprises support 1, and magnet base 2 is placed on support 1, comprises a locking latches on support, in the time the separation vessel 200 of biological sample is housed is placed on magnet base, locking latches can be locked at separation vessel 200 on magnetic frame.Magnetic part is installed on magnet base.
In one embodiment, magnetic frame locking latches comprises clip and baffle plate assembly.A clip 4 is installed in one end of locking latches, and the other end is equipped with a baffle plate assembly.After separation vessel 200 is placed on magnet base 2, the one end of first clip 4 being clamped to separation vessel, is then resisted against baffle plate assembly the other end of separation vessel, thereby separation vessel is fixed on magnetic frame.Due to some separation vessel, for example standard template also has bottom plate eaves (the similar the brim of a hat), and clip 4 and baffle plate assembly can also reach by blocking plate eaves the object of retaining plate.Described baffle plate assembly comprises a push pedal 6, screw 7 and cutting die 8.In the time that screw 7 screws inward, promote push pedal 6 and rely on separation vessel, thereby coordinate separation vessel is fixed on magnetic frame tightly with clip.The separation vessel being locked just can not fall down from magnetic frame, and the operation of being inverted like this separation vessel just can complete.
In another embodiment as in Figure 3-5, magnetic frame locking latches is shell fragment 9 and the shell fragment support 10 that is arranged on magnetic frame two ends, and shell fragment is arranged on shell fragment support, and shell fragment comprises elastic arm 11 and shell foot 12.Angle between elastic arm 11 and shell foot 12 is greater than 90 degree.Shell foot bottom surface 13 does not touch the upper surface of magnet base, and the distance between shell foot bottom surface 13 and magnet base upper surface is just in time the height of separation vessel.In the time that separation vessel 200 is put into magnetic frame along shell foot, the wooden partition of separation vessel 200 has applied an outside power to shell foot, and extruded shell foot opens to both sides, makes separation vessel descending smoothly.When separation vessel is during lower than shell foot, shell foot returns to home position after losing outside thrust, and shell foot bottom surface just in time mortgages on separation vessel and compressed separation vessel, thereby separation vessel is fixed on magnetic frame.While needing to take out separation vessel after lock out operation completes, apply a power shell foot is no longer resisted against on separation vessel to shell foot, separation vessel 200 just can be taken away from magnetic frame smoothly like this.The design of the present embodiment makes the placement of separation vessel and is fixed in a step to complete simultaneously, and does not need multiple other fixing step.
In an embodiment as shown in Fig. 1 and Fig. 6, be provided with magnetic part holding tank 15 in magnet base 2, magnetic holding tank is parallel each other.Magnetic part leaves in this holding tank, and magnetic part can long strip shape or circle.In one embodiment, magnetic part is to be bonded in holding tank with glue.Embodiment as shown in Figure 6, holding tank 15 is a convex shape cavity, and magnetic part 30 is a convex shape, and the convex shape of magnetic part is less than the convex shape of holding tank.Two shoulders 31 of magnetic part are just in time resisted against the internal surface 5 of magnet base 2, and magnetic part is in without the hydropexic situation of glue like this, and magnetic part can be from the upper shed of holding tank 16 through skidding off.In the time that whole magnet base 2 is installed on support, magnetic part just can not come off.Because this mode is not used glue, so in the time changing the magnetic part damaging, directly the magnetic part damaging is taken out from magnet base, and do not need to remove arduously glue.Magnetic part is installed also very convenient, as long as the shoulder of magnetic part 31 is pushed in the cavity of holding tank.In order further to strengthen stability and the stability of magnetic part, can shoulder 31 be fixedly connected with magnet base 2 by modes such as screws.
In a preferred scheme, the magnetic part of convex shape is not that tool is magnetic all over, and it comprises base plate 33 and magnet 32, and base plate 33 is non-magnetic substances, and magnet 32 is fixed on base plate 33.Magnet 32 is through holding tank 15, and two shoulders 31 of base plate are just in time resisted against the internal surface 5 of magnet base 2, and magnet just can not skid off from holding tank.Magnet base 2 can be bonded on support 1 with glue etc., and in a preferred scheme, magnet base is to be fixed on support with mounting block 30, for example, use screw means on support, and convenient so i.e. dismounting is conducive to again change magnetic part.
In one embodiment, the opening surface of the magnetic part holding tank on magnet base is not parallel with magnet base bottom surface, but angled, and it is also angled with magnet base bottom surface to be placed on magnetic part surface in holding tank, and such design just increases the area for magnetic absorption.In the mode shown in Fig. 7, the magnet contact surface 35 that magnetic part contacts with separation vessel is rendered as inclined-plane, and the bottom outer wall 210 of inclined-plane and separator 200 is just in time fitted, and magnetic part just has more area to touch the outer wall of separation vessel like this.Shown in Fig. 7, on magnet base, the position that each separation vessel is corresponding is provided with two magnetic part holding tanks, and the extended line of two holding tank openings can intersect, and holding tank opening is an inclined-plane like this.After the magnet coordinating with this holding tank is put into wherein, magnet and separator contact surface are an inclined-plane.Such design can also allow the separation vessel being placed on it more firm.
When use, magnet base 2 is fixed on support 1.On magnet base, can also comprise support hole 40, for example, at the bottom of the pipe of the each extraction tube of 96 hole PCR plate, just in time drop in support hole, thereby prevent that 96 hole PCR plates are moved on magnetic frame.
Described biological specimen separation vessel is selected from 96 hole PCR plates, enzyme plate, 24 holes, 48 holes, 96 hole depth orifice plates, reservoir etc.
Described magnet is the materials such as neodymium permanent magnet material, electromagnetism, cobalt nickel magnet, can be used for adsorbing magnetic particle in biological magnetic lock out operation, thereby realizes target molecule ground sharp separation and purifying in complex mixture.
Embodiment 1
Separate nucleic acid system in biological specimen, comprises magnetic frame 100 described in the utility model and KBM separate nucleic acid reagent.Described separate nucleic acid reagent comprises: damping fluid ME1, rinsing liquid Wash1D, rinsing liquid Wash2A, Proteinase K, magnetic bead suspension B eads C, elution buffer Eluent B and Virahol.
The operation steps of utilizing biological specimen separate nucleic acid system described in the utility model to extract nucleic acid comprises:
1. in 96 deep-well plates, add the Proteinase K of 20ul;
2. add sample;
3. in every hole, add 450ul damping fluid ME1, lash to mix or vibrate and mix;
4. 70 DEG C of water-baths, 20 minutes, digesting material;
5. every hole adds 100ul magnetic bead suspension B eads C and 400ul Virahol, lashes to mix or vibrate to mix 5 minutes;
6. 96 deep-well plates are placed on magnetic frame and leave standstill 30 seconds, in the time that magnetic bead adsorbs completely, carefully remove liquid; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 deep-well plates on thieving paper, abandon to the greatest extent raffinate.
7. deep-well plates is taken off from magnetic frame, add 750ul rinsing liquid Wash1D, lash to mix or vibrate and mix;
8. 96 deep-well plates are placed on magnetic frame, 96 orifice plates are left standstill to 30 seconds on magnetic frame, the careful liquid of removing in the time that magnetic bead adsorbs completely; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 orifice plates on thieving paper, abandon to the greatest extent raffinate.
9. repeating step 7-8.
10. deep-well plates is taken off from magnetic frame, add 1000ul rinsing liquid Wash1D, lash to mix or vibrate and mix;
11. are placed on 96 deep-well plates on magnetic frame, and 96 orifice plates are left standstill to 30 seconds on magnetic frame, the careful liquid of removing in the time that magnetic bead adsorbs completely; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 deep-well plates on thieving paper, abandon to the greatest extent raffinate.
12. repeating steps 10 and 11;
13. room temperatures are dried or heat drying 10-15 minute;
14. take off deep-well plates from magnetic frame, add the elution buffer Eluent B of 80-250ul, lash to mix or vibrate to mix 5 minutes;
15. are placed on deep-well plates on magnetic frame and leave standstill 30 seconds, in the time that magnetic bead adsorbs completely, carefully DNA solution are transferred to collecting board, and preserve or complete follow-up test experience in felicity condition.
Result shows:
1. analyze by ultraviolet spectrophotometer, the sample DNA purity that adopts this magnetic frame to extract with reagent is consistent with the sample DNA purity of pellosil method extraction.As shown in table 1.
Table 1 ultraviolet spectrophotometer is analyzed the sample DNA purity that two kinds of methods are extracted
2. analyze by agarose gel electrophoresis and gel imaging instrument, the sample DNA that adopts this magnetic frame and reagent to extract must be measured the sample DNA extracting than pellosil method and must measure higher.M representation DNA molecular weight standard; Column represents pellosil extracting method; KBM represents KBM paramagnetic particle method extracting method.
3. detect analysis by PCR, in the sample DNA that adopts this magnetic frame and reagent to extract, do not contain PCR inhibitor.Column represents pellosil extracting method; KBM represents KBM paramagnetic particle method extracting method; P represents positive sample.
To sum up result shows, the DNA purity of utilizing method described in the utility model to obtain is high, can be directly used in PCR, equimolecular biological experiment is cut, hybridized to enzyme.Be held on magnetic frame in 96 deep-well plates, liquid is abandoned in upset, and can firmly overturn and pat deep-well plates on thieving paper, abandons to the greatest extent in the operating process of raffinate, and deep-well plates can not drop from magnetic frame.Whole operation is highly suitable for manual extraction, simple and convenient.
Claims (6)
1. sample extraction device, comprise magnetic frame, described magnetic frame comprises support, be placed on the magnet base on support, magnetic part is installed in magnet base, locking latches has been installed on support, it is characterized in that, described locking latches comprises the shell fragment and the shell fragment support that are arranged on magnetic frame two ends, and shell fragment is arranged on shell fragment support.
2. device according to claim 1, is characterized in that, described shell fragment comprises elastic arm and shell foot.
3. device according to claim 2, is characterized in that, the angle between elastic arm and shell foot is greater than 90 degree.
4. device according to claim 2, is characterized in that, the shell foot bottom surface of described shell foot does not touch the upper surface of magnet base.
5. device according to claim 4, is characterized in that, the distance between shell foot bottom surface and magnet base is just in time the height of separation vessel.
6. device according to claim 1, is characterized in that, also comprises support hole on magnet base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420160894.6U CN203878151U (en) | 2014-04-01 | 2014-04-01 | Sample extractor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420160894.6U CN203878151U (en) | 2014-04-01 | 2014-04-01 | Sample extractor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203878151U true CN203878151U (en) | 2014-10-15 |
Family
ID=51678673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420160894.6U Expired - Lifetime CN203878151U (en) | 2014-04-01 | 2014-04-01 | Sample extractor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203878151U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190827A (en) * | 2015-05-07 | 2016-12-07 | 深圳华大基因科技服务有限公司 | For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus |
| CN109443870A (en) * | 2018-10-31 | 2019-03-08 | 重庆英特力科技有限公司 | Cell centrifugal rotor |
-
2014
- 2014-04-01 CN CN201420160894.6U patent/CN203878151U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190827A (en) * | 2015-05-07 | 2016-12-07 | 深圳华大基因科技服务有限公司 | For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus |
| CN109443870A (en) * | 2018-10-31 | 2019-03-08 | 重庆英特力科技有限公司 | Cell centrifugal rotor |
| CN109443870B (en) * | 2018-10-31 | 2021-05-28 | 重庆英特力科技有限公司 | Cell centrifugal rotor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203030295U (en) | Test tube for mixing and extracting liquid | |
| US10704039B2 (en) | Device and method for extracting nucleic acids | |
| JP2016502862A5 (en) | ||
| CN202237381U (en) | Novel, simple and easy solid phase extract device | |
| WO2012134440A1 (en) | Methods and devices for nucleic acid purification | |
| CN210448175U (en) | Push rod type filtering purification column | |
| CN203878151U (en) | Sample extractor | |
| CN203878152U (en) | Magnetic frame for separating biological samples | |
| CN204325349U (en) | A kind of instrument for extracting nucleic acid | |
| CN102507297A (en) | Microwave-assisted Soxhlet and solid-phase extraction coupling device, microwave-assisted Soxhlet and solid-phase extraction coupling method and application of microwave-assisted Soxhlet and solid-phase extraction coupling | |
| CN105861493A (en) | Free DNA extraction kit and application thereof | |
| CN203878136U (en) | Magnetic system for separating biological samples | |
| CN203999628U (en) | Sample splitter | |
| CN103994914A (en) | Novel method for extracting trace heavy metal ion in environmental water sample | |
| CN201020402Y (en) | Detachable utility type magnetic separating rack | |
| CN203855572U (en) | Instrument for extracting active substance of organisms | |
| CN108004233A (en) | A kind of negative pressure device available for automatic instrument for extracting nucleic acid | |
| CN203355863U (en) | Magnetic separation device for separating biological material | |
| CN206279200U (en) | A kind of townhouse pipe and matching used waste collecting device | |
| CN207516132U (en) | A kind of multi-functional Tip SPE of high throughput ultramicron | |
| CN203598534U (en) | Solid-phase extraction device | |
| CN103923817B (en) | A kind of micro-fluidic DNA extraction system of linear pattern detected towards PCR | |
| CN204325348U (en) | A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus | |
| CN202141701U (en) | Small column for solid phase extraction processing | |
| CN204910865U (en) | Novel solid -phase extraction system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: Hangzhou City, Zhejiang province 311228 Jiangdong Industrial Zone Linjiang high tech Zone weft five road No. 3688 building four layer 2 Branch Park Patentee after: HANGZHOU KBM LIFE SCIENCES CO.,LTD. Address before: 310007, A, East 628, Zhejiang Science Park, 525 Xixi Road, Hangzhou, Zhejiang, Xihu District Patentee before: HANGZHOU KBM LIFE SCIENCES CO.,LTD. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141015 |