CN203999628U - Sample splitter - Google Patents
Sample splitter Download PDFInfo
- Publication number
- CN203999628U CN203999628U CN201420160922.4U CN201420160922U CN203999628U CN 203999628 U CN203999628 U CN 203999628U CN 201420160922 U CN201420160922 U CN 201420160922U CN 203999628 U CN203999628 U CN 203999628U
- Authority
- CN
- China
- Prior art keywords
- magnetic
- separation vessel
- magnet base
- holding tank
- magnetic part
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000005291 magnetic effect Effects 0.000 claims abstract description 94
- 238000000926 separation method Methods 0.000 claims abstract description 53
- 239000012472 biological sample Substances 0.000 abstract description 11
- 239000000523 sample Substances 0.000 abstract description 11
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 18
- 239000007788 liquid Substances 0.000 description 11
- 238000000605 extraction Methods 0.000 description 7
- 239000011324 bead Substances 0.000 description 6
- 239000003292 glue Substances 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 239000012634 fragment Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000012445 acidic reagent Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 229910052779 Neodymium Inorganic materials 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- ZGDWHDKHJKZZIQ-UHFFFAOYSA-N cobalt nickel Chemical compound [Co].[Ni].[Ni].[Ni] ZGDWHDKHJKZZIQ-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a kind of sample splitter, comprises support, is placed on the magnet base on support, is provided with magnetic part holding tank in magnet base, and the opening surface of holding tank is not parallel with magnet base bottom surface.The area that magnetic frame described in the utility model has increased magnetic absorption is greatly large, from having improved biological sample separation efficiency and separation purity.
Description
Technical field
The utility model relates to biological sample separation field, relates in particular to a kind of by the separator of magneticaction separation of biological samples.
Background technology
Magnetic separation technique has a wide range of applications in cytobiology, immunology, diagnostics and pharmacy etc. separated, can realize the object of the materials such as fast separating and purifying cell, albumen or nucleic acid.Compare as the precipitator method, centrifuging, ion exchange column method with conventional partition method, magnetic separation technique has that separating device is few, velocity of separation fast, extraction efficiency high.
Magnetic frame is adsorbable magnetic particle in biological magnetic lock out operation, thereby realizes sharp separation and the purifying of target molecule in complex mixture, is a kind of simple in structure, easy to operate magnetic separating apparatus.During use, will the separation vessel of biological sample be housed, for example 96 orifice plates, put into magnetic frame.Magnetic substance in sample will be enriched on the magneticsubstance in separation agent, and is fitted on the tube wall of separation vessel.Remove not by the material of magnetic absorption, and through obtaining the biological sample of purifying after repeatable operation repeatedly.But existing magnetic frame is only used for support separation vessel, the separation vessel of putting thereon can not be fixed.In extraction from biological material process, if upset separation vessel, existing magnetic frame just can not complete.Because when magnetic frame overturns, separation vessel can drop from magnetic frame, so existing magnetic frame can not adapt to more lock out operation.
Utility model content
For overcoming the deficiencies in the prior art, the purpose of this utility model is to provide a kind of sample splitter, comprises support, is placed on the magnet base on support, is provided with magnetic part holding tank in magnet base, and the opening surface of holding tank is not parallel with magnet base bottom surface.
Further, magnetic part is installed in holding tank, the face that described magnetic part contacts with separation vessel is an inclined-plane.
Further, on magnet base, also comprise support hole.
Further, on support, also comprise locking latches.
Further, locking latches comprises clip and baffle plate assembly.
The beneficial effects of the utility model are: magnetic frame described in the utility model can be fixed the separation vessel of putting thereon, in extraction from biological material process, if upset separation vessel, separation vessel just can not drop from magnetic frame, so the operating easily of biological extraction.Locking latches described in the utility model makes the placement of 96 orifice plates and is fixed in a step to complete simultaneously, and does not need to increase extra fixing step.When magnetic part adopts convex shape structure, the combination between magnetic part and magnet base just does not need to fix with glue.When magnetic part is impaired, can completes more and change jobs easily like this.Design described in the utility model has increased the contact area of magnet and separation vessel, increases the area of magnetic absorption, has improved the separation efficiency of biological sample.
Accompanying drawing explanation
Fig. 1 is magnetic frame structural representation.
Fig. 2 is the magnetic frame structural representation that separation vessel is housed.
Fig. 3 is that separation vessel is at the first location schematic diagram of elastic piece structure locking latches.
Fig. 4 is that separation vessel is at the second position schematic diagram of elastic piece structure locking latches.
Fig. 5 is that separation vessel is at the 3rd position view of elastic piece structure locking latches.
Fig. 6 is convex shape magnetic part holding tank structural representation.
Fig. 7 is the magnetic part holding tank structural representation of tool ramp structure.
Embodiment
Below in conjunction with concrete accompanying drawing, the utility model is described in detail.These specific embodiments are only limited the enumerating under the utility model spirit, do not get rid of one of ordinary skill in the art prior art and the utility model in conjunction with and other specific embodiments of producing.
As illustrated in fig. 1 and 2, magnetic frame 100 for separating of biological sample comprises support 1, and magnet base 2 is placed on support 1, comprises a locking latches on support, when the separation vessel 200 of biological sample being housed being placed on magnet base, locking latches can be locked at separation vessel 200 on magnetic frame.Magnetic part is installed on magnet base.
In one embodiment, magnetic frame locking latches comprises clip and baffle plate assembly.A clip 4 is installed in one end of locking latches, and the other end is equipped with a baffle plate assembly.After separation vessel 200 is placed on magnet base 2, the one end of first clip 4 being clamped to separation vessel, is then resisted against baffle plate assembly the other end of separation vessel, thereby separation vessel is fixed on magnetic frame.Due to some separation vessel, for example standard template also has bottom plate eaves (the similar the brim of a hat), and clip 4 and baffle plate assembly can also reach by blocking plate eaves the object of retaining plate.Described baffle plate assembly comprises a push pedal 6, screw 7 and cutting die 8.When screw 7 screws inward, promote push pedal 6 and rely on separation vessel, thereby coordinate separation vessel is fixed on magnetic frame tightly with clip.The separation vessel being locked just can not fall down from magnetic frame, and the operation of being inverted like this separation vessel just can complete.
In another embodiment as in Figure 3-5, magnetic frame locking latches is shell fragment 9 and the shell fragment support 10 that is arranged on magnetic frame two ends, and shell fragment is arranged on shell fragment support, and shell fragment comprises elastic arm 11 and shell foot 12.Angle between elastic arm 11 and shell foot 12 is greater than 90 degree.Shell foot bottom surface 13 does not touch the upper surface of magnet base, and the distance between shell foot bottom surface 13 and magnet base upper surface is just in time the height of separation vessel.When separation vessel 200 is put into magnetic frame along shell foot, the wooden partition of separation vessel 200 has applied an outside power to shell foot, and extruded shell foot opens to both sides, makes separation vessel descending smoothly.When separation vessel is during lower than shell foot, shell foot returns to home position after losing outside thrust, and shell foot bottom surface just in time mortgages on separation vessel and compressed separation vessel, thereby separation vessel is fixed on magnetic frame.While needing to take out separation vessel after lock out operation completes, apply a power shell foot is no longer resisted against on separation vessel to shell foot, separation vessel 200 just can be taken away from magnetic frame smoothly like this.The design of the present embodiment makes the placement of separation vessel and is fixed in a step to complete simultaneously, and does not need a plurality of other fixing step.
In an embodiment as shown in Fig. 1 and Fig. 6, be provided with magnetic part holding tank 15 in magnet base 2, magnetic holding tank is parallel each other.Magnetic part leaves in this holding tank, and magnetic part can long strip shape or circle.In one embodiment, magnetic part is to be bonded in holding tank with glue.Embodiment as shown in Figure 6, holding tank 15 is a convex shape cavity, and magnetic part 30 is a convex shape, and the convex shape of magnetic part is less than the convex shape of holding tank.Two shoulders 31 of magnetic part are just in time resisted against the internal surface 5 of magnet base 2, and magnetic part is in the hydropexic situation without glue like this, and magnetic part can be from the upper shed 16 of holding tank through skidding off.When whole magnet base 2 is installed on support, magnetic part just can not come off.Because this mode is not used glue, so when changing the magnetic part damaging, directly the magnetic part damaging is taken out from magnet base, and do not need to remove arduously glue.Magnetic part is installed also very convenient, as long as the shoulder of magnetic part 31 is pushed in the cavity of holding tank.In order further to strengthen stability and the stability of magnetic part, can shoulder 31 be fixedly connected with magnet base 2 by modes such as screws.
In a preferred scheme, the magnetic part of convex shape is not that tool is magnetic all over, and it comprises base plate 33 and magnet 32, and base plate 33 is non-magnetic substances, and magnet 32 is fixed on base plate 33.Magnet 32 is through holding tank 15, and two shoulders 31 of base plate are just in time resisted against the internal surface 5 of magnet base 2, and magnet just can not skid off from holding tank.Magnet base 2 can be bonded on support 1 with glue etc., and in a preferred scheme, magnet base is to be fixed on support with mounting block 30, for example, use screw means on support, and convenient so i.e. dismounting is conducive to again change magnetic part.
In one embodiment, the opening surface of the magnetic part holding tank on magnet base is not parallel with magnet base bottom surface, but angled, and the magnetic part surface being placed in holding tank is also angled with magnet base bottom surface, and such design just increases the area for magnetic absorption.In the mode shown in Fig. 7, the magnet contact surface 35 that magnetic part contacts with separation vessel is rendered as inclined-plane, and the bottom outer wall 210 of inclined-plane and separator 200 is just in time fitted, and magnetic part just has more area to touch the outer wall of separation vessel like this.Shown in Fig. 7, on magnet base, the position that each separation vessel is corresponding is provided with two magnetic part holding tanks, and the extended line of two holding tank openings can intersect, and holding tank opening is an inclined-plane like this.After the magnet coordinating with this holding tank is put into wherein, magnet and separator contact surface are an inclined-plane.Such design can also allow the separation vessel being placed on it more firm.
During use, magnet base 2 is fixed on support 1.On magnet base, can also comprise support hole 40, for example, at the bottom of the pipe of 96 hole each extraction tube of PCR plate, just in time drop in support hole, thereby prevent that 96 hole PCR plates are moved on magnetic frame.
Described biological sample separation vessel is selected from 96 hole PCR plates, enzyme plate, 24 holes, 48 holes, 96 hole depth orifice plates, reservoir etc.
Described magnet is the materials such as neodymium permanent magnet material, electromagnetism, cobalt nickel magnet, can be used for adsorbing magnetic particle in biological magnetic lock out operation, thereby realizes target molecule ground sharp separation and purifying in complex mixture.
Embodiment 1
Separate nucleic acid system in biological sample, comprises magnetic frame 100 described in the utility model and KBM separate nucleic acid reagent.Described separate nucleic acid reagent comprises: damping fluid ME1, rinsing liquid Wash1D, rinsing liquid Wash2A, Proteinase K, magnetic bead suspension B eads C, elution buffer Eluent B and Virahol.
The operation steps of utilizing biological sample separate nucleic acid system described in the utility model to extract nucleic acid comprises:
1. in 96 deep-well plates, add the Proteinase K of 20ul;
2. add sample;
3. in every hole, add 450ul damping fluid ME1, lash to mix or vibrate and mix;
4. water-bath is 70 ℃, and 20 minutes, digesting material;
5. every hole adds 100ul magnetic bead suspension B eads C and 400ul Virahol, lashes to mix or vibrate to mix 5 minutes;
6. 96 deep-well plates are placed on magnetic frame to standing 30 seconds, when magnetic bead adsorbs completely, carefully remove liquid; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 deep-well plates on thieving paper, abandon to the greatest extent raffinate.
7. deep-well plates is taken off from magnetic frame, add 750ul rinsing liquid Wash1D, lash to mix or vibrate and mix;
8. 96 deep-well plates are placed on magnetic frame, by 96 orifice plates on magnetic frame standing 30 seconds, the careful liquid of removing when magnetic bead adsorbs completely; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 orifice plates on thieving paper, abandon to the greatest extent raffinate.
9. repeating step 7-8.
10. deep-well plates is taken off from magnetic frame, add 1000ul rinsing liquid Wash1D, lash to mix or vibrate and mix;
11. are placed on 96 deep-well plates on magnetic frame, by 96 orifice plates on magnetic frame standing 30 seconds, and the careful liquid of removing when magnetic bead adsorbs completely; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 deep-well plates on thieving paper, abandon to the greatest extent raffinate.
12. repeating steps 10 and 11;
13. room temperatures are dried or heat drying 10-15 minute;
14. take off deep-well plates from magnetic frame, add the elution buffer Eluent B of 80-250ul, lash to mix or vibrate to mix 5 minutes;
15. are placed on magnetic frame standing 30 seconds by deep-well plates, when magnetic bead adsorbs completely, carefully DNA solution are transferred to collecting board, and preserve or complete follow-up test experience in felicity condition.
Result shows:
1. by ultraviolet spectrophotometer, analyze, the sample DNA purity that adopts this magnetic frame to extract with reagent is consistent with the sample DNA purity of pellosil method extraction.As shown in table 1.
Table 1 ultraviolet spectrophotometer is analyzed the sample DNA purity that two kinds of methods are extracted
2. by agarose gel electrophoresis and gel imaging instrument, analyze, the sample DNA that adopts this magnetic frame and reagent to extract must be measured the sample DNA extracting than pellosil method and must measure higher.M representation DNA molecular weight standard; Column represents pellosil extracting method; KBM represents KBM paramagnetic particle method extracting method.
3. by PCR, detect analysis, in the sample DNA that adopts this magnetic frame and reagent to extract, do not contain PCR inhibitor.Column represents pellosil extracting method; KBM represents KBM paramagnetic particle method extracting method; P represents positive.
To sum up result shows, the DNA purity of utilizing method described in the utility model to obtain is high, can be directly used in PCR, equimolecular biological experiment is cut, hybridized to enzyme.In 96 deep-well plates, be held on magnetic frame, liquid is abandoned in upset, and can firmly overturn and pat deep-well plates on thieving paper, abandons to the greatest extent in the operating process of raffinate, and deep-well plates can not drop from magnetic frame.Whole operation is highly suitable for manual extraction, simple and convenient.
Claims (5)
1. a sample splitter, comprises support, is placed on the magnet base on support, is provided with magnetic part holding tank in magnet base, it is characterized in that, the opening surface of described holding tank is not parallel with magnet base bottom surface.
2. sample splitter according to claim 1, is characterized in that, magnetic part is installed in holding tank, and the face that described magnetic part contacts with separation vessel is an inclined-plane.
3. sample splitter according to claim 1, is characterized in that, also comprises support hole on magnet base.
4. sample splitter according to claim 1, is characterized in that, also comprises locking latches on support.
5. sample splitter according to claim 4, is characterized in that, locking latches comprises clip and baffle plate assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420160922.4U CN203999628U (en) | 2014-04-01 | 2014-04-01 | Sample splitter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420160922.4U CN203999628U (en) | 2014-04-01 | 2014-04-01 | Sample splitter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203999628U true CN203999628U (en) | 2014-12-10 |
Family
ID=52039657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420160922.4U Expired - Lifetime CN203999628U (en) | 2014-04-01 | 2014-04-01 | Sample splitter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203999628U (en) |
-
2014
- 2014-04-01 CN CN201420160922.4U patent/CN203999628U/en not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203030295U (en) | Test tube for mixing and extracting liquid | |
| CN105524917A (en) | Kit for extracting blood genome DNA based on magnetic bead method and use method for kit | |
| CN203745435U (en) | Special sleeve centrifugal QuEChERS purifier | |
| US20110114487A1 (en) | Apparatus, method, and gel system for analytical and preparative electrophoresis | |
| CN202237381U (en) | Novel, simple and easy solid phase extract device | |
| CN210448175U (en) | Push rod type filtering purification column | |
| CN203878152U (en) | Magnetic frame for separating biological samples | |
| CN203878151U (en) | Sample extractor | |
| CN203878136U (en) | Magnetic system for separating biological samples | |
| CN203999628U (en) | Sample splitter | |
| CN201020402Y (en) | Detachable utility type magnetic separating rack | |
| CN203355863U (en) | Magnetic separation device for separating biological material | |
| CN103901139A (en) | Pretreatment method for analyzing tetrabromobisphenol A in biologic urine | |
| CN203598534U (en) | Solid-phase extraction device | |
| CN206279200U (en) | A kind of townhouse pipe and matching used waste collecting device | |
| CN207516132U (en) | A kind of multi-functional Tip SPE of high throughput ultramicron | |
| CN103923817B (en) | A kind of micro-fluidic DNA extraction system of linear pattern detected towards PCR | |
| CN105624149A (en) | Kit and method for extracting and purifying DNA (deoxyribonucleic acid) in untouchable specimen | |
| CN205518197U (en) | Biological (DNA) draws special magnetic resolution frame | |
| CN202141701U (en) | Small column for solid phase extraction processing | |
| CN103901143A (en) | Pretreatment method for analyzing tetrabromobisphenol A in small amount of biologic serum | |
| CN204325348U (en) | A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus | |
| CN205074084U (en) | Modular small -size desktop formula magnetic separation device of high flux | |
| CN210065759U (en) | Simple and easy efficient cell separation device | |
| CN104280491B (en) | A kind of analytical approach detecting 11 kinds of ionic liquid cation in soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Hangzhou City, Zhejiang province 311228 Jiangdong Industrial Zone Linjiang high tech Zone weft five road No. 3688 building four layer 2 Branch Park Patentee after: HANGZHOU KBM LIFE SCIENCES CO.,LTD. Address before: 310007, A, East 628, Zhejiang Science Park, 525 Xixi Road, Hangzhou, Zhejiang, Xihu District Patentee before: HANGZHOU KBM LIFE SCIENCES CO.,LTD. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141210 |