CN106190827A - For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus - Google Patents
For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus Download PDFInfo
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- CN106190827A CN106190827A CN201510229670.5A CN201510229670A CN106190827A CN 106190827 A CN106190827 A CN 106190827A CN 201510229670 A CN201510229670 A CN 201510229670A CN 106190827 A CN106190827 A CN 106190827A
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- putting hole
- centrifuge tube
- card division
- plate body
- body card
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Abstract
The invention discloses a kind of magnetic frame for separating biological sample, it includes plate body and magnetic part, this plate body offers the putting hole of cylinder, this magnetic part is arranged on the side of this putting hole, the inwall of this putting hole is provided with plate body card division, and this plate body card division is for coordinating to prevent this centrifuge tube from rotating in this putting hole with the body card division of centrifuge tube.In above-mentioned magnetic frame, the inwall of putting hole is provided with plate body card division, coordinate to prevent centrifuge tube from rotating in putting hole with the body card division of centrifuge tube, therefore, above-mentioned magnetic frame decreases the disturbance to magnetic bead because of the rotation of centrifuge tube during magnetic frame uses, and can also improve the accuracy of experiment while convenient operation.Invention additionally discloses a kind of centrifuge tube and a kind of separation apparatus.
Description
Technical field
The present invention relates to biology field, more specifically, relate to a kind of for separating biological sample
Magnetic frame and a kind of centrifuge tube and a kind of separate apparatus.
Background technology
Along with PCR, RT-PCR equimolecular biology techniques is advanced by leaps and bounds, immunomagnetic beads is possible not only to point
From protein, and can be by Avidin-Biotin system so that immunomagnetic beads produces with various PCR
Thing DNA moleculars etc. combine, and for separating and the corresponding DNA of purification, are not required to centrifugal and filter, joint
Make an appointment and avoid manual operation overlong time and cause the loss of product.
Along with sample high-flux sequence development, many magnetic beads for purifying reactors arise at the historic moment, and such as 96
Orifice plate magnetic frame.96 orifice plate magnetic frames are once tested and can be carried out 96 Sample Purification on Single operations.But due to
The PCR pipe position being placed on 96 orifice plates is not fixed, PCR in being purified operating process
Rotating often occurs in pipe, and the magnetic bead that PCR pipe is adsorbed after rotating is temporarily deviate from magnetic part, at the work of magnetic force
Under with, magnetic bead moves to magnetic stripe again, has resulted in the disturbance of magnetic bead.When magnetic bead generation disturbance, need
Just can carry out next step operation when magnetic bead to be waited is stablized again, reduce scientific research efficiency.If magnetic bead
Occur disturbance not found just to carry out subsequent operation in time, usually can produce experimental repeatability reduction etc.
Consequence.
Summary of the invention
It is contemplated that at least solve one of technical problem present in prior art.To this end, the present invention needs
A kind of magnetic frame for separating biological sample and a kind of centrifuge tube and a kind of separation apparatus are provided.
A kind of magnetic frame for separating biological sample, including plate body and magnetic part, this plate body offers circle
The putting hole of cylindricality, this magnetic part is arranged on the side of this putting hole, and the inwall of this putting hole is provided with plate
Body card division, this plate body card division is for coordinating to prevent this centrifuge tube in this placement with the body card division of centrifuge tube
Rotate in hole.
In above-mentioned magnetic frame, the inwall of putting hole is provided with plate body card division, joins with the body card division of centrifuge tube
Closing to prevent centrifuge tube from rotating in putting hole, therefore, above-mentioned magnetic frame decreases and used at magnetic frame
The disturbance to magnetic bead because of the rotation of centrifuge tube in journey, can also improve the standard of experiment while convenient operation
Really property.
In one embodiment, these putting holes multiple are that ranks are distributed on this plate body, and adjacent two arrange this
This magnetic part it is provided with between putting hole.
In one embodiment, arrange in this putting hole adjacent two, along two these plates with a line distribution
Body card division is oppositely arranged along the radial direction of this putting hole, along the line of two these card divisions, plate position with a line distribution
It is perpendicular to this magnetic part.
In one embodiment, this plate body card division is the strip groove of the inwall being opened in this putting hole,
This strip groove extends along the opening of this putting hole to the bottom of this putting hole.
A kind of centrifuge tube for separating biological sample, including body and body card division, this body card division sets
Putting on the outer wall of this body, this body offers containing cavity, and this body card division is for the plate with magnetic frame
Body card division coordinates to prevent this centrifuge tube from rotating in this magnetic frame.
In one embodiment, this body card division is raised line.
In one embodiment, this centrifuge tube includes lid, and this lid connects outer wall the energy of this body
Enough sealings and this containing cavity open.
In above-mentioned centrifuge tube, body card division can coordinate to prevent centrifuge tube from putting with the plate body card division of magnetic frame
Putting in hole and rotate, therefore, above-mentioned centrifuge tube decreases during centrifuge tube uses because of the rotation of centrifuge tube
And the disturbance to magnetic bead, the accuracy of experiment can also be improved while convenient operation.
A kind of separation apparatus for separating biological sample, including magnetic frame and centrifuge tube, this magnetic frame bag
Rubbing board body and magnetic part, this plate body offers the putting hole of cylinder, and this putting hole is used for placing this and is centrifuged
Pipe, this magnetic part is arranged on the side of this putting hole, and the inwall of this putting hole is provided with plate body card division.Should
Centrifuge tube includes body and body card division, and this body card division is arranged on the outer wall of this body, and this body is opened
Being provided with containing cavity, this body card division is for coordinating to prevent this centrifuge tube at this putting hole with this plate body card division
Middle rotation.
In one embodiment, this body card division is raised line, and this plate body card division is for being opened in this putting hole
The strip groove of inwall, this strip groove extends along the opening of this putting hole to the bottom of this putting hole,
This raised line is positioned in this strip groove.
In one embodiment, this centrifuge tube includes lid, and this lid connects outer wall the energy of this body
Enough sealings and this containing cavity open.
In above-mentioned separation apparatus, body card division can coordinate to prevent centrifuge tube from existing with the plate body card division of magnetic frame
Rotating in putting hole, therefore, above-mentioned separation apparatus decreases during centrifuge tube uses because of centrifuge tube
Rotate and disturbance to magnetic bead, the accuracy of experiment while convenient operation, can also be improved.
The additional aspect of the present invention and advantage will part be given in the following description, and part is by from following
Description becomes obvious, or recognized by the practice of the present invention.
Accompanying drawing explanation
Embodiment is retouched by the above-mentioned and/or additional aspect of the present invention and advantage from combining accompanying drawings below
Will be apparent from easy to understand in stating, wherein:
Fig. 1 is the schematic perspective view separating apparatus of better embodiment of the present invention；
Fig. 2 is the decomposing schematic representation separating apparatus of better embodiment of the present invention；
Fig. 3 is the enlarged diagram of the separation apparatus III part of Fig. 2；
Fig. 4 is the separation apparatus partial sectional schematic view along IV-IV line of Fig. 3；
Fig. 5 is the schematic perspective view of the centrifuge tube of better embodiment of the present invention；
Fig. 6 is the centrifuge tube schematic cross-section along line VI--VI of Fig. 5.
Detailed description of the invention
Embodiments of the present invention are described below in detail, and the example of this embodiment is shown in the drawings, its
In the most same or similar label represent same or similar element or there is same or like merit
The element of energy.The embodiment described below with reference to accompanying drawing is exemplary, is only used for explaining this
Bright, and be not considered as limiting the invention.
In describing the invention, it is to be understood that term " center ", " longitudinally ", " laterally ",
" length ", " width ", " thickness ", " going up ", " under ", " front ", " afterwards ", " left ",
" right ", " vertically ", " level ", " top ", " end ", " interior ", " outward ", " up time
The orientation of the instructions such as pin ", " counterclockwise " or position relationship be based on orientation shown in the drawings or pass, position
System, be for only for ease of describe the present invention and simplify describe rather than instruction or hint indication device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this
The restriction of invention.Additionally, term " first ", " second " are only used for describing purpose, and it is not understood that
For indicating or imply relative importance or the implicit quantity indicating indicated technical characteristic.Thus, limit
Surely have " first ", the feature of " second " can express or implicitly include one or more should
Feature.In describing the invention, " multiple " are meant that two or more, unless otherwise bright
The most concrete restriction.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, term
" install ", " being connected ", " connection " should be interpreted broadly, and connect, also for example, it may be fixing
Can be to removably connect, or be integrally connected；Can be mechanically connected, it is also possible to be electrical connection or can
With mutual communication；Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, can be two
The connection of element internal or the interaction relationship of two elements.For those of ordinary skill in the art
Speech, can understand above-mentioned term concrete meaning in the present invention as the case may be.
In the present invention, unless otherwise clearly defined and limited, in second feature, it " goes up " fisrt feature
Or it " under " can include that the first and second features directly contact, it is also possible to include the first and second features
It not directly contact but by the other characterisation contact between them.And, fisrt feature is second
Feature " on ", " top " and " above " include fisrt feature directly over second feature and oblique on
Side, or it is merely representative of fisrt feature level height higher than second feature.Fisrt feature is at second feature " it
Under ", " lower section " and " below " include fisrt feature directly over second feature and oblique upper, or only
Only represent that fisrt feature level height is less than second feature.
Following disclosure provides many different embodiments or example for realizing the difference of the present invention
Structure.In order to simplify disclosure of the invention, hereinafter parts and setting to specific examples are described.
Certainly, they are the most merely illustrative, and are not intended to limit the present invention.Additionally, the present invention can be
Repeat reference numerals and/or reference letter in different examples, this repetition is to simplify and clearly mesh
, itself do not indicate the relation between discussed various embodiment and/or setting.Additionally, this
The bright various specific technique provided and the example of material, but those of ordinary skill in the art can anticipate
Know the application to other techniques and/or the use of other materials.
Please join Fig. 1, the separation apparatus 100 for separating biological sample of better embodiment of the present invention
Including magnetic frame 102 and centrifuge tube 104.This separation apparatus 100 can be used for such as PCR (Polymerase
Chain Reaction, Polymerase chain reaction formula is reacted) class reaction experiment, separate sample liquid and magnetic bead.
This centrifuge tube 104 can be used for carrying out PCR class reaction.
Magnetic frame 102 is used for placing centrifuge tube 104.Magnetic frame 102 includes plate body 106 and magnetic part
108, plate body 106 offers the putting hole 110 of cylinder.This magnetic part 108 is arranged on this placement
The side in hole 110.Plate body 106 is made up of nonmagnetic substance, such as plastics etc..
In present embodiment, plate body 106 is 96 orifice plates, i.e. plate body 106 offers 96 cylinders
Putting hole 110, these 96 putting holes 110 are distributed in ranks on this plate body 106, as one
Individual example, the distribution mode that 96 putting holes 110 arrange in 8 row * 12 is opened on plate body 106.Can
To understand, in other embodiments, the quantity of putting hole 110 can be other quantity, and is not limited to
96.
Incorporated by reference to Fig. 2, being arranged with 2 by 12 putting holes 110 arranged is one group, is divided into 6 groups.From the right side to
A left side, numbered N1, N2, N3, N4, N5 and N6 of group.
Magnetic part 108 is magnetic stripe.One magnetic part 108 is arranged on adjacent two and arranges between this putting hole 110,
Two row being the most often provided with in a magnetic part 108, i.e. group N1 in group putting hole 110 are put
Put and between hole 110, be provided with a magnetic part 108, as shown in Figure 3.Two row in group N2 are placed
It is provided with between hole 110 between two row putting holes 110 in a magnetic part 108 ..., group N6
It is provided with a magnetic part 108 (in Fig. 2, to observe for convenience, only demonstrate magnetic between group N1
Part 108, and the overall structure of two putting holes 110, be specifically shown in Fig. 3).And two adjacent groups putting hole
Magnetic part 108 it is not provided with between 110.It is arranged such, on the one hand can reduce the use of magnetic part 108
Amount, reduces cost, on the other hand, can reduce in adjacent two magnetic parts 108, a magnetic part 108
The magnetic bead being acted on another magnetic part 108 produces interference.
Magnetic part 108 can select Magnet or other magnetic part.Magnetic part 108 can be parallel to putting hole
The central shaft of 110 is arranged.The inwall of each putting hole 110 is provided with plate body card division 112.This enforcement
In mode, this plate body card division 112 is the strip groove of the inwall being opened in this putting hole 110, this
Connected in star extends along the opening of this putting hole 110 to the bottom of this putting hole 110.Strip groove length
Bearing of trend be parallel to the central shaft of putting hole 110.Strip groove can be substantially in rectangular-shaped
Strip groove.
Further, describe for convenience, by adjacent two row putting holes 110 and be positioned at adjacent two row and put
Put magnetic part 108 between hole as a separative element, such as group N1 and the magnetic part that is positioned between group N1
108 as a separative element, and so on.
In a separative element, along two plate body card divisions 112 with a line distribution along this putting hole 110
Radial direction oppositely arranged, be perpendicular to magnetic part along with the line of two plate body card divisions 112 of a line distribution
108, specifically, in conjunction with Fig. 4, as in group N1, edge is with two plate body card divisions 112 of a line distribution
Oppositely arranged along the radial direction of putting hole 110, and the line of two plate body card divisions 112 is perpendicular to be positioned at group
Magnetic part 108 between N1.Oppositely arranged refer to, in a separative element, a putting hole 110
In the plate body card division 112 of plate body card division 112 and another putting hole 110 being adjacent all towards far
On the inwall that the direction of magnetic part 108 is arranged on putting hole 110 (as shown in Figure 4).So set
Put, the impact that the magnetic bead in centrifuge tube 104 is produced by plate body card division 112 can be reduced.
Please join Fig. 5, centrifuge tube 104 includes body 114 and body card division 116.This body card division 116
It is arranged on the outer wall of this body 114.
Specifically, joining Fig. 6, this body 114 offers containing cavity 118, and containing cavity 118 can be used for
Accommodating sample liquid and the mixture of magnetic bead.This body 114 includes cylindrical portion 120 and conus portion 122,
Cylindrical portion 120 connects conus portion 122.
This body card division 116 is arranged on the outer wall of cylindrical portion 120.When centrifuge tube 104 is placed in placement
Time in hole 110, body card division 116 can coordinate with plate body card division 112 to prevent this centrifuge tube 104 from existing
This magnetic frame 102 rotates.
In present embodiment, this body card division 116 is raised line.When centrifuge tube 104 is placed in putting hole 110
Time interior, raised line is positioned in strip groove to prevent the centrifuge tube 104 rotation in putting hole 110.Convex
The shape of bar and the mating shapes of strip groove, i.e. raised line and strip groove coordinate play prevent from
While heart pipe 104 rotates, it is also possible to facilitate centrifuge tube 104 picking and placeing in putting hole 110.
Being appreciated that in other embodiments, body card division 116 and plate body card division 112 can be it
Its shape or the card division of structure, prevent centrifuge tube 104 from putting as long as the cooperation of two card divisions can be played
While rotating in putting hole 110, it is also convenient for centrifuge tube 104 picking and placeing in putting hole 110.
Further, in other embodiments, body card division 116 is alternatively opened in body 114 outer wall
Groove, plate body card division 112 is alternatively arranged on the raised line in putting hole 110.
It addition, in order to prevent sample liquid from spilling leakage in operating kind, centrifuge tube 104 also includes lid 124,
This lid 124 connects the outer wall of this body 114 and can seal and open containing cavity 118.Specifically,
Lid 124 is connected on the outer wall of cylindrical portion 120 by soft connecting portion 126.
Further, body card division 116 also can effectively control magnetic frame with coordinating of plate body card division 112
The direction that on 102, centrifuge tube 104 is placed so that the direction that these centrifuge tubes 104 are placed is consistent,
During operation very convenient, greatly reduce operation easier.
The use separating apparatus of present embodiment is described with concrete experiment below.
Experiment: 96 orifice plate PCR, the purification procedures of RT-PCR product includes:
1. resuspended AmPure XP Beads under room temperature, takes appropriate magnetic bead in 1: 1 ratio and joins PCR
In centrifuge tube 104, piping and druming 10 times, make amplified production in PCR pipe 104 the most mixed with magnetic bead gently
Even, incubated at room temperature 8min.
2. all PCR centrifuge tubes 104 are positioned on 96 orifice plate anti-magnetic bead disturbance magnetic frame 102,
The body card division 116 making PCR pipe side is mounted to plate body card division 112, the position quilt of PCR pipe 104
Fixing；Absorption 5min, after liquid is clarified, sucks.
3. in the case of not disturbance magnetic bead, add 200ul 80% ethanol, after standing 30s, suck,
Repeated washing one time；
4. under room temperature, being dried 3～5min, magnetic bead can take off centrifuge tube 104 after having slight slight crack,
Adding appropriate EB (or NF-Water), piping and druming mixing, room temperature stands 2min；
The most anti-magnetic bead disturbance magnetic frame 102 adsorbs 2min, the centrifuge tube 104 of taking-up liquid a to cleaning
In；
6. take 1ul for Qubit, 2100 detections, then take 5ul for expanding electrophoresis detection.Close
Lattice result is: band is at 500bp-2.2kb, wherein, there will be near 1.5-2kb
One peak.
In above-mentioned experimentation, owing to magnetic frame 102 prevents centrifuge tube 104 at putting hole 110
In rotation, it is to avoid the disturbance of magnetic bead so that the accuracy of experiment improves, and decreases operation simultaneously
Difficulty.
In sum, in above-mentioned separation apparatus, the inwall of the putting hole of magnetic frame is provided with plate body card division,
Can coordinate to prevent centrifuge tube from rotating in putting hole with the body card division of centrifuge tube, therefore, above-mentioned separation
Apparatus decreases the disturbance to magnetic bead because of the rotation of centrifuge tube during magnetic frame uses, convenient operation
While can also improve the accuracy of experiment.
In the description of this specification, reference term " embodiment ", " some embodiments ",
The description of " exemplary embodiment ", " example ", " concrete example " or " some examples " etc.
Mean that the specific features, structure, material or the feature that combine this embodiment or example description are contained in this
In at least one embodiment of invention or example.In this manual, the schematic table to above-mentioned term
State and be not necessarily referring to identical embodiment or example.And, the specific features of description, structure, material
Material or feature can combine in any one or more embodiments or example in an appropriate manner.
While embodiments of the present invention have been illustrated and described, those of ordinary skill in the art is permissible
Understand: can these embodiments be carried out multiple in the case of without departing from the principle of the present invention and objective
Changing, revise, replace and modification, the scope of the present invention is limited by claim and equivalent thereof.
Claims (10)
1. the magnetic frame being used for separating biological sample, it is characterised in that include plate body and magnetic part,
This plate body offers the putting hole of cylinder, and this magnetic part is arranged on the side of this putting hole, this putting hole
Inwall be provided with plate body card division, this plate body card division is for coordinating to prevent this with the body card division of centrifuge tube
Centrifuge tube rotates in this putting hole.
2. the magnetic frame for separating biological sample as claimed in claim 1, it is characterised in that many
This putting hole individual is that ranks are distributed on this plate body, and adjacent two arrange and are provided with this magnetic part between this putting hole.
3. the magnetic frame for separating biological sample as claimed in claim 2, it is characterised in that
Adjacent two arrange in this putting hole, anti-along the radial direction of this putting hole along two these plate body card divisions with a line distribution
To setting, the line along two these card divisions, plate position with a line distribution is perpendicular to this magnetic part.
4. the magnetic frame for separating biological sample as claimed in claim 1, it is characterised in that should
Plate body card division is the strip groove of the inwall being opened in this putting hole, this strip groove opening along this putting hole
Mouth extends to the bottom of this putting hole.
5. the centrifuge tube being used for separating biological sample, it is characterised in that include body and body card
Portion, this body card division is arranged on the outer wall of this body, and this body offers containing cavity, this body card division
Prevent this centrifuge tube from rotating in this magnetic frame for coordinating with the plate body card division of magnetic frame.
6. the centrifuge tube for separating biological sample as claimed in claim 5, it is characterised in that should
Body card division is raised line.
7. the centrifuge tube for separating biological sample as claimed in claim 5, it is characterised in that should
Centrifuge tube includes lid, and this lid connects the outer wall of this body and can seal and this containing cavity open.
8. for separating a separation apparatus for biological sample, including magnetic frame and centrifuge tube, its feature
Being, this magnetic frame includes plate body and magnetic part, and this plate body offers the putting hole of cylinder, this placement
Hole is used for placing this centrifuge tube, and this magnetic part is arranged on the side of this putting hole, and the inwall of this putting hole sets
It is equipped with plate body card division；
This centrifuge tube includes body and body card division, and this body card division is arranged on the outer wall of this body, should
Body offers containing cavity, and this body card division is for coordinating to prevent this centrifuge tube at this with this plate body card division
Putting hole rotates.
9. the separation apparatus for separating biological sample as claimed in claim 8, it is characterised in that
This body card division is raised line, and this plate body card division is the strip groove of the inwall being opened in this putting hole, this
Connected in star extends along the opening of this putting hole to the bottom of this putting hole, and this raised line is positioned at this strip groove
In.
10. the separation apparatus for separating biological sample as claimed in claim 8, it is characterised in that
This centrifuge tube includes lid, and this lid connects the outer wall of this body and can seal and this containing cavity open.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510229670.5A CN106190827A (en) | 2015-05-07 | 2015-05-07 | For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510229670.5A CN106190827A (en) | 2015-05-07 | 2015-05-07 | For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106190827A true CN106190827A (en) | 2016-12-07 |
Family
ID=57459896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510229670.5A Pending CN106190827A (en) | 2015-05-07 | 2015-05-07 | For separating magnetic frame and the centrifuge tube of biological sample and separating apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106190827A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102274790A (en) * | 2011-05-09 | 2011-12-14 | 广州华峰生物科技有限公司 | Clamping type magnetic separation frame |
| CN202277894U (en) * | 2011-07-29 | 2012-06-20 | 新疆康地种业科技股份有限公司 | Combined type magnetic separation device |
| CN203355863U (en) * | 2013-07-09 | 2013-12-25 | 生工生物工程（上海）股份有限公司 | Magnetic separation device for separating biological material |
| CN203725254U (en) * | 2014-01-24 | 2014-07-23 | 百欧生命科技有限公司 | Magnetic separation rack |
| CN203737383U (en) * | 2013-12-10 | 2014-07-30 | 无锡百运纳米科技有限公司 | Multifunctional magnetic separation rack |
| CN203878151U (en) * | 2014-04-01 | 2014-10-15 | 杭州百迈生物技术有限公司 | Sample extractor |
| CN204307734U (en) * | 2014-11-10 | 2015-05-06 | 天津恒瑞捷科技发展有限公司 | A kind of centrifuge rotor device |
-
2015
- 2015-05-07 CN CN201510229670.5A patent/CN106190827A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102274790A (en) * | 2011-05-09 | 2011-12-14 | 广州华峰生物科技有限公司 | Clamping type magnetic separation frame |
| CN202277894U (en) * | 2011-07-29 | 2012-06-20 | 新疆康地种业科技股份有限公司 | Combined type magnetic separation device |
| CN203355863U (en) * | 2013-07-09 | 2013-12-25 | 生工生物工程（上海）股份有限公司 | Magnetic separation device for separating biological material |
| CN203737383U (en) * | 2013-12-10 | 2014-07-30 | 无锡百运纳米科技有限公司 | Multifunctional magnetic separation rack |
| CN203725254U (en) * | 2014-01-24 | 2014-07-23 | 百欧生命科技有限公司 | Magnetic separation rack |
| CN203878151U (en) * | 2014-04-01 | 2014-10-15 | 杭州百迈生物技术有限公司 | Sample extractor |
| CN204307734U (en) * | 2014-11-10 | 2015-05-06 | 天津恒瑞捷科技发展有限公司 | A kind of centrifuge rotor device |
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Application publication date: 20161207 |
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| RJ01 | Rejection of invention patent application after publication |