CN201149590Y - Large active force cell strain loading device under three-dimensional cultivation condition - Google Patents
Large active force cell strain loading device under three-dimensional cultivation condition Download PDFInfo
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- CN201149590Y CN201149590Y CNU200820028172XU CN200820028172U CN201149590Y CN 201149590 Y CN201149590 Y CN 201149590Y CN U200820028172X U CNU200820028172X U CN U200820028172XU CN 200820028172 U CN200820028172 U CN 200820028172U CN 201149590 Y CN201149590 Y CN 201149590Y
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Abstract
The utility model discloses a large acting force cell strain loading device in three-dimensional culture conditions, a power supply is connected to electrodes of a piezoelectric ceramic chip driving portion through a drive wire, and the electrodes are connected with a piezoelectric stacking driver; the piezoelectric stacking driver is fixed on a substrate by a ceramic chip fixing device, the rear end of a displacement transferring plunger is fixed with the displacement output end of the piezoelectric stacking driver, a three-dimensional compound body composed of a cell support is positioned at the central location at the bottom part of a culture chamber, the left and right ends thereof are respectively connected with a porous fixed material, one side of the porous fixed material is fixed on the wall of the culture chamber, and the other side of the porous fixed material is fixedly connected with the displacement transferring plunger; and a laser probe is non-contact mounted on the rear end of the transferring plunger and connected with a computer, and detects the backward and forward displacement of the transferring plunger. The loading device can load the amplitude and frequency without closing down the machine and can be adjusted conveniently; and has advantages of good maneuverability, high repeatability precision, stable mechanical loading, low error and avoiding magnetic-field interference.
Description
Technical field
The present invention relates to a kind of determinator that is used for biomechanics Research, cell is accepted the loading experimental apparatus of mechanical stress stimulation under particularly a kind of three-dimensional cultivation condition.
Background technology
In recent years, increasing along with crossing research between life science and other subjects, the new branch of science that biomechanics is carried out quantitative examination as applied mechanics principle and method to the mechanics problem in the biosome becomes the focus of field of biology research gradually.Wherein cell is one of field, forward position of present biomechanics Research to the response investigations of mechanical stimulation.Yet because the human body internal milieu is extremely complicated, the comparatively difficulty that research also becomes in the body of this aspect.And multiple isolated cells culture technique provides possibility for the biological effect of studying mechanical stimulation on the cell aspect.Studies show that mechanical stimulation can cause cytoskeleton to produce deformation, cause the variation of skeleton arrangement, and then influence the many aspects such as mechanical property, adhesive force, propagation, differentiation of cellular morphology, cell self.
Many isolated cells Experiments of Machanics methods have been studied personnel's proposition and have been used for the research of biosome to the mechanism aspects such as response of mechanics factor, as substrate tension, four-point bending stretching, hydrostatic pressure and fluid shear stress stimulation separately, the combination of fluid shear stress etc.Also set up simultaneously the model that many mechanics load.Publication number is a kind of cyto-mechanics loading system that the Chinese patent of CN 1847847 discloses, this system is made of the culture chamber that the bottom is fixed with pellosil, can inoculating cell on the pellosil, hoisting gasbag is arranged at the pellosil bottom, by adjusting how many generation distortion of air bag internal gas,, pellosil produces planar equal-biaxial periodical pull stress or compressive stress thereby being out of shape.Though this device is simple to operate, volume is little, the distortion that external force produces air bag is closely related with the original pressure of its internal gas, is difficult to accomplish therefore that very accurate control air bag is out of shape; Secondly the deformation that cell receives in this device does not have direct detection system.Publication number be CN 1425905 patented invention a kind of four point bending cell mechanics loader, this device is by the rotation of stepper motor, drive drive screw and produce the front and back displacement, promote pressure head (two contact points respectively being arranged up and down) and load the culture plate that inoculation has cell, make culture plate and cell produce distortion with Tissue Culture Plate.This device controllability is good, the repeatable accuracy height, owing to need to use but device is comparatively complicated, and because the deformation difference of culture plate diverse location produces identical deformation so can not guarantee cell.Publication number be CN 1932510 patent disclosure a kind of charger of cell tensile, it passes through controller, the driver control stepper motor rotates, rotation is changed into the displacement of horizontal direction by screw mandrel, the pulling inoculation has the culture membrane of cell, make cell be subjected to the stretcher strain of horizontal direction, this device can make cell stressed comparatively even, accurately control stretching amplitude and frequency, stretching distance also can be regulated easily, but use stepper motor in this device as drive source, inevitably bring the interference in magnetic field, and magnetic field is still indeterminate at present as a kind of influence of physical factor pair cell, studies cell for us the response of mechanical stimulation is brought disturbing factor.
Above-mentioned three disclosed devices of patent all are based upon cell monolayer and cultivate on the basis, the cultured cell monolayer growth, comparatively convenient when carrying out as experiments such as morphologic observation, cell dyeings, but the residing solid space of cell under the shortage internal milieu, weakened influencing each other and acting on of iuntercellular autocrine, paracrine, therefore and the environment of body cell when being subjected to mechanical stimulation is 3 D stereo, should set up the research model of cell under the 3 D stereo condition during research aspect carrying out this.Publication number be CN 1567399 patent disclosure cardiac muscle cell's mechanical stimulation device under a kind of three-dimensional cultivation condition, this device utilizes method of tissue engineering to make up cell--the complex of collagen, form the dimensional culture environment of cell, by motor, flywheel, connecting rod and slide block the rotation of motor is become motion on the horizontal direction again, periodic cell--the complex of collagen that loads makes cell be subjected to mechanical stimulation.Though this device has been realized the three dimensional growth environment of cell, it drives the motor that produces magnetic field when still using work, and its adjusting that loads amplitude is needed to shut down, and brings inconvenience to operation; In addition, this device adopts too much mechanical drive, and also there is the wear problem in the long-term work in unavoidable noise and the heat of producing during work.
Summary of the invention
For overcome the prior art complex structure, have magnetic field, the deficiency of aspects such as disturbing factor such as heat; the invention provides cell or tissue strain loading device under a kind of three-dimensional cultivation condition; simple in structure; can not shut down and change parameters such as loading amplitude, frequency; avoid magnetic interference; the generation heat is low, is applicable to the mechanics loading experimental apparatus of cell under the three-dimensional cultivation condition.
The technical solution adopted for the present invention to solve the technical problems is: adopt piezoelectric ceramic piece as drive source, the three-dimensional complex that pair cell--porous support constitutes carries out mechanical stimulation.The present invention is primarily aimed at that pair cell carries out mechanical stimulation under the three-dimensional cultivation condition, comprises drive power supply for piezoelectric ceramics, piezoelectric ceramic piece drive part, cell culture unit and laser displacement sensor deformation test section.The piezoelectric ceramic piece drive part mainly is made of its core component piezoelectric ceramic piece, electrode, potsherd stationary installation, transmission plunger, porous immobilization material; Cell culture unit is made of substrate and cultivation cell; Laser displacement sensor deformation test section is made up of laser probe and computing machine.Described piezoelectric ceramic piece adopts technology maturation, the convenient piezo-electric stack driver of buying, and can realize the cell bigger to hardness--the loading of porous support complex.Power supply is connected on the electrode of piezo-electric stack driver by drive wire, by the potsherd stationary installation piezo-electric stack driver is fixed on the cell culture unit, the displacement output terminal that plunger one end is fixed on the piezo-electric stack driver is transmitted in displacement, the other end with is connected cell--prop up the porous immobilization material connection of the three-dimensional complex that is configured to.Cell--a three-dimensional complex that is configured to is positioned at cultivates cell bottom center position, about connect the porous immobilization material respectively, the porous immobilization material of a side is fixed on to be cultivated on the little locular wall, the porous immobilization material of opposite side is fixedlyed connected with displacement transmission plunger.The laser probe noncontact is installed on the transmission plunger back-end, links to each other with computing machine, detects the front and back displacement of transmitting plunger.
Described porous immobilization material is not for producing Cytotoxic porosint, as porous polyethylene etc.
Cell culture unit adopts organic glass or polytetrafluoroethylmaterial material to make among the present invention, is easy to processing, also can sterilize easily simultaneously, prevents that possible pollution from taking place.
Principle of work of the present invention: drive power supply for piezoelectric ceramics provides the voltage of sinusoidal wave or other waveforms of piezo-electric stack driver needs, the flexural deformation that the piezo-electric stack driver produces or the anteroposterior position Mobile Communication of piezo-electric stack driver cross displacement transmission plunger and are delivered to cell--on the porous support complex, cell--porous support complex two ends condense together with the porous immobilization material respectively, so that can produce compression or stretcher strain when plunger seesaws, and then make cell produce periodic deformation.By with transmit the laser probe that the plunger noncontact is installed, detect the displacement that transmits plunger, data transfer by the processing of computer software, shows the displacement waveform to computing machine in real time.
The invention has the beneficial effects as follows: the present invention can pair cell applies the deformation of waveforms such as sine wave or triangular wave, frequency range is 0-100Hz, amplitude of deformation is that maximum can reach 1mm, and adopt the method that makes up porous support and cell conjugate to provide the cell growth required three-dimensional environment, farthest simulated the growing environment in the cell body.This device adopts the piezo-electric stack driver as the power that loads cell; can under non-stop-machine situation, load amplitude, frequency; and can regulate easily, piezo-electric stack driver acting force is bigger simultaneously, can realize the loading of the cell bigger to hardness～porous support complex.
Stretching, compressive strain that the output of cyto-mechanics loading experimental apparatus among the present invention produces various ways, it is good to have operability, the repeatable accuracy height, mechanics loads steadily, and error is little, the magnetic interference of having brought when having avoided stepper motor as drive source.The characteristics such as 3 D stereo culture environment of cell growth needs can be provided, the deformation that cell is produced, the experiment under the same terms has good consistance.Can be used as pair cell in the biomechanics and carry out the experimental provision of mechanical stimulation response investigations.
The present invention is further described below in conjunction with drawings and Examples.
Description of drawings
Fig. 1 is that big acting force cell three-dimensional of the present invention is cultivated the strain loading device synoptic diagram;
Among the figure, 1-driver rack, 2-power supply, 3-drive wire, 4-set bolt, 5-piezo-electric stack driver, 6-cultivates cell, and 7-transmits plunger, and the 8-plunger is connected and fixed pin, the complex of 9-collagen scaffold and cell, 10-porous immobilization material, 11-laser probe, 12-computing machine.
Fig. 2 is that big acting force cell three-dimensional of the present invention is cultivated the unit three-dimensional synoptic diagram.
Embodiment
Embodiment:
See figures.1.and.2, apparatus of the present invention comprise drive power supply for piezoelectric ceramics, piezoelectric ceramic piece drive part, cell culture unit and laser displacement sensor deformation test section.Drive power supply for piezoelectric ceramics 2 provides on-load voltage by drive wire 3 for the piezoelectric ceramic piece drive part; The piezoelectric ceramic piece drive part mainly is made of its core component piezo-electric stack driver 5, electrode, potsherd stationary installation, transmission plunger 7, porous immobilization material 10; Cell culture unit is made of substrate and cultivation cell 6; Laser displacement sensor deformation test section is made up of laser probe 11 and computing machine 12.Wherein driver rack 1 is selected in substrate for use, and the potsherd stationary installation is selected set bolt 4 for use, and porous immobilization material 10 uses porous polyethylene, and driver rack 1, set bolt 4, cultivation cell 6 are pmma material, and transmitting plunger 7 materials is teflon.Driver rack 1 is the base of this device, cultivates cell 6 and is fixed on the driver rack 1.Use piezo-electric stack driver 5 as drive source, driving force is provided.The transmission of displacement realizes by transmitting plunger 7.Piezo-electric stack driver 5 usefulness set bolts 4 are fixed in the driver rack 1, will transmit plunger 7 and be connected piezo-electric stack driver 5 vibration output terminals.The each several part that transmits plunger 7 is connected and fixed pin 8 with plunger and connects.The other end that transmits plunger be connected the porous immobilization material 10 of collagen scaffold and be connected with the complex 9 of cell.The complex 9 of collagen scaffold and cell is positioned at cultivates cell bottom center position, about connect porous immobilization material 10, one sides respectively porous immobilization material 10 be fixed on and cultivate on cell 6 walls, the porous immobilization material 10 of opposite side is fixedlyed connected with transmission plunger 7.When pair cell applies mechanical stimulation in the experiment, at first regulate power supply 2 output required voltage signals, the corresponding deformation of piezo-electric stack driver 5 outputs is transmitted the complex 9 generation distortion that make collagen scaffold and cell by transmitting plunger 7.Laser probe 11 noncontacts are installed in the place ahead of transmitting plunger 7, are used to collect the deformation signal that transmits plunger 7, and signal obtains parameters such as detected vibrational waveform and amplitude through computing machine 12 software processes.
Claims (3)
1, big acting force cell strain loading device under the three-dimensional cultivation condition, comprise drive power supply for piezoelectric ceramics, piezoelectric ceramic piece drive part, cell culture unit and laser displacement sensor deformation test section, it is characterized in that: described piezoelectric ceramic piece drive part comprises piezoelectric ceramic piece, electrode, potsherd stationary installation, transmits plunger, porous immobilization material; Cell culture unit is made of substrate and cultivation cell; Laser displacement sensor deformation test section comprises laser probe and computing machine; Described piezoelectric ceramic piece adopts the piezo-electric stack driver, power supply is connected on the electrode of piezo-electric stack driver by drive wire, by the potsherd stationary installation piezo-electric stack driver is fixed on the cell culture unit, the displacement output terminal that plunger one end is fixed on the piezo-electric stack driver is transmitted in displacement, the other end with is connected cell--prop up the porous immobilization material connection of the three-dimensional complex that is configured to; Cell--a three-dimensional complex that is configured to is positioned at cultivates cell bottom center position, about connect the porous immobilization material respectively, the porous immobilization material of a side is fixed on to be cultivated on the little locular wall, the porous immobilization material of opposite side is fixedlyed connected with displacement transmission plunger; The laser probe noncontact is installed on the transmission plunger back-end, links to each other with computing machine, detects the front and back displacement of transmitting plunger.
2, according to utilizing big acting force cell strain loading device under the described three-dimensional cultivation condition of claim 1, it is characterized in that: described porous immobilization material is not for producing Cytotoxic porosint.
3, according to utilizing big acting force cell strain loading device under the described three-dimensional cultivation condition of claim 1, it is characterized in that: described cell culture unit adopts organic glass or polytetrafluoroethylmaterial material to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200820028172XU CN201149590Y (en) | 2008-01-22 | 2008-01-22 | Large active force cell strain loading device under three-dimensional cultivation condition |
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| Application Number | Priority Date | Filing Date | Title |
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| CNU200820028172XU CN201149590Y (en) | 2008-01-22 | 2008-01-22 | Large active force cell strain loading device under three-dimensional cultivation condition |
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| CN201149590Y true CN201149590Y (en) | 2008-11-12 |
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| CNU200820028172XU Expired - Fee Related CN201149590Y (en) | 2008-01-22 | 2008-01-22 | Large active force cell strain loading device under three-dimensional cultivation condition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN102268360A (en) * | 2011-04-27 | 2011-12-07 | 南开大学 | Supersonic vibration microscopic cell penetrating system based on piezoelectric ceramic driving |
| CN111830295A (en) * | 2019-04-18 | 2020-10-27 | 云谷（固安）科技有限公司 | Device for testing electric performance of micro-element |
-
2008
- 2008-01-22 CN CNU200820028172XU patent/CN201149590Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN102268360A (en) * | 2011-04-27 | 2011-12-07 | 南开大学 | Supersonic vibration microscopic cell penetrating system based on piezoelectric ceramic driving |
| CN102268360B (en) * | 2011-04-27 | 2013-01-30 | 南开大学 | Supersonic vibration microscopic cell penetrating system based on piezoelectric ceramic driving |
| CN111830295A (en) * | 2019-04-18 | 2020-10-27 | 云谷（固安）科技有限公司 | Device for testing electric performance of micro-element |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081112 Termination date: 20120122 |