CN1720813A - Process for preparing active peptide of corn protein - Google Patents
Process for preparing active peptide of corn protein Download PDFInfo
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- CN1720813A CN1720813A CNA2004100437096A CN200410043709A CN1720813A CN 1720813 A CN1720813 A CN 1720813A CN A2004100437096 A CNA2004100437096 A CN A2004100437096A CN 200410043709 A CN200410043709 A CN 200410043709A CN 1720813 A CN1720813 A CN 1720813A
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Abstract
The invention provides a process for preparing corn protein active peptides by using any one, two or the mixture of corn protein meal, corn syrup and yellow syrup as raw material, which comprises the following steps, charging water to regulate the concentration of the raw material to 10-15%, adjusting pH to 6.5-7.0, carrying out sterilization treatment under high temperature and heavy pressure, cooling down to 30 deg. C, inducing bacteria with a content of 5-10% of total fermentation liquid amount, stirring homogeneously, carrying out 70-80 hours of fermentation treatment, keeping the fermentation temperature at 30+-2 deg. C, agitating continuously, carrying out high temperature disinfection after the end of fermentation, then centrifugalizing to concentrate the supernatant fluid to 20-30Bx, finally spray drying into end product.
Description
(1), affiliated field
What the present invention relates to is the processing method of a kind of food or feed, specifically a kind of processing method of edible or feed protein matter.
(2), background technology
Modern scientific research shows, small-molecular peptides has higher biologically active and nutritive value than the amino acid of equivalent, peptide class and amino acid almost all can be absorbed and used by the Epithelium of intestinal villus cell fully, enter into blood, be transported to each position of organism then, protein then must be through just being absorbed and used after the digestion.The peptide class is by the free amino acid of organism infiltration rate faster than equivalent, greatly faster than the protein of equivalent.Contain a certain amount of protein in the corn, its commercial form is corn protein powder or corn steep liquor, and zein is difficult to be digested and assimilated by organism based on alcohol soluble protein, generally all is used for the nitrogenous source of feed or fermentation industry, can't well develop.If be translated into small-molecular peptides, will improve the utilization rate of zein greatly, it is not soluble in water thoroughly to have solved zein, the problem that is difficult to digest and assimilate.The method that at present zein is converted into corn protein peptide mainly is that acid hydrolyzation and enzymatic isolation method are arranged.The acid hydrolyzation reaction is violent, and adds strong acid, HTHP, and reflection process and end product all are difficult to control, can cause a large amount of amino acid whose destructions, and cause environmental pollution; Though the enzyme process reaction condition is gentle, the production cost height, the final products bitter taste is obvious, and mouthfeel is relatively poor.
(3), summary of the invention
The object of the present invention is achieved like this: with corn protein powder, corn steep liquor, the mixture of any in the yellow seriflux or two kinds or three kinds is a raw material, adding water earlier sizes mixing to material concentration 10-15%, the accent pH value is 6.5-7.0, then at high temperature, carry out sterilization treatment under the high pressure, be cooled to 30 ℃, the 5-10% that presses the zymotic fluid total amount inserts bacterium, stir, carry out 70-80 hour fermentation process, temperature remains on 30 ℃ ± 2 ℃ during fermentation, and constantly stirs, carry out high-temperature sterilization after the fermentation ends, centrifugation then is concentrated into 20-30Bx again with supernatant, and spray-drying is made finished product.
The present invention can also comprise some features like this:
1, described zymogenous bacteria is a bacillus, method for culturing bacteria is: take by weighing the 6g corn protein powder, claim 3.85g glucose again, the 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, add 90ml water, stir, sterilized 30 minutes for 121 ℃, be cooled to 30 ℃, insert seed, stir, 30 ℃ of Aseptic Culture 24 hours.
2, the high-temperature sterilization of raw material is to sterilize 30 minutes under 121 ℃ ± 2 ℃ temperature.
3, the high-temperature sterilization after the fermentation is to sterilize 15 minutes under 121 ℃ ± 2 ℃ temperature.
The present invention with any in corn protein powder, corn steep liquor, the yellow seriflux or wherein the mixture of two kinds or three kinds be raw material, adopt inoculated bacteria, method by microbial fermentation turns to the zein active peptide with the protein transduction in the corn, little molecule corn peptide not only has good dissolubility, low viscosity, flowability that high concentration is good, and absorb in vivo fast, utilization rate is high.The zein active peptide is except nutritious, easy absorption, also have anti-hypertension, anti-cholesterol, antithrombotic forms, improve lipid-metabolism, eliminate fattiness, prevented artery sclerosis, strengthened function of human body and muscle strength, promote the flesh red blood cell to restore and help to set up, promote calcium phosphorus and other trace elements to absorb, remove free radical, non-oxidizability, play the blood street cleaner.Corn peptide can also promote brain and neurodevelopment, raising memory.Corn peptide also has and strengthens phagocyte and B cell viability, strengthen immunologic function, anticancer, anti-ageing, protect epidermal cell, prevent melanin deposition, promote cell proliferation.The protection liver also helps liver function recovery, and the diabetes patient is had prevention and therapeutic action, can prevent the generation of senile dementia, senile osteoporosis and gall stone.According to these functions, the zein active peptide can be used as additive or hardening agent, add in the Foods or drinks, the special peptide milk powder of system baby, pregnant woman, health care for the middle and old aged corn peptide pulvis, electuary, tablet, capsule, corn peptide infant foods, drink, breakfast for students food stuff improving intelligence, drink, the anti-ageing food of preserving youthful looks of women, drink, the corn peptide brute force is replenished the calcium, phosphorus, micro-absorbent, drinks such as corn peptide health-care spirit, beer, yellow rice wine, corn peptide beauty care cosmetics, high-quality microbiological culture media etc.Process reaction temperature of the present invention and, than being easier to control, the impurity content of its final products is low, peculiar smell such as no bitterness, and its production cost is lower.
(4), specific embodiments
Carry out the cultivation of bacillus earlier, carry out the preparation of fermentation medium and the fermentation of corn peptide again, method for culturing bacteria is: take by weighing the 6g corn protein powder, claim 3.85g glucose again, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, add 90ml water, stir, sterilized 30 minutes for 121 ℃, be cooled to 30 ℃, insert seed, stir, 30 ℃ of Aseptic Culture 24 hours, detect qualified after, finish as the Bacteria Culture of seed liquor.
The preparation of fermentation medium and fermentation process are: take by weighing the 100g corn protein powder, add 900ml water, stir, transfer PH6.5-7.0, sterilized 30 minutes for 121 ℃, be cooled to 30 ℃, insert 75ml bacterium seed liquor, stir, cultivated 72 hours for 30 ℃, sweat constantly stirs, and fermentation is after 18 hours, per 12 hours analytical tests once, after the fermentation to 54 hour, per 6 hours analytical tests are once measured a-amino acid and corn peptide content, when the a-amino acid content reaches about 10%, the 55-65% that corn peptide content accounts for total protein content promptly reaches the fermentation ends index.After reaching the fermentation ends index, 121 ℃ of sterilizations 15 minutes, centrifugation then, the supernatant evaporation and concentration is to 25Bx, the spray-dried product of making.
Claims (5)
1, a kind of preparation method of zein active peptide, it is characterized in that: with corn protein powder, corn steep liquor, the mixture of any in the yellow seriflux or two kinds or three kinds is a raw material, adding water earlier sizes mixing to material concentration 10-15%, the accent pH value is 6.5-7.0, then at high temperature, carry out sterilization treatment under the high pressure, be cooled to 30 ℃, the 5-10% that presses the zymotic fluid total amount inserts bacterium, stirs, and carries out 70-80 hour fermentation process, temperature remains on 30 ℃ ± 2 ℃ during fermentation, and constantly stir, carry out high-temperature sterilization, centrifugation then after the fermentation ends, supernatant is concentrated into 20-30Bx again, and spray-drying is made finished product.
2, the preparation method of zein active peptide according to claim 1 is characterized in that: described zymogenous bacteria is a bacillus, and method for culturing bacteria is: take by weighing the 6g corn protein powder, claim 3.85g glucose again, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate adds 90ml water, stir, sterilized 30 minutes for 121 ℃, be cooled to 30 ℃, insert seed, stir 30 ℃ of Aseptic Culture 24 hours.
3,, the preparation method of zein active peptide according to claim 1 and 2, it is characterized in that: the high-temperature sterilization of described raw material is sterilization 30 minutes under 121 ℃ ± 2 ℃ temperature.
4, the preparation method of zein active peptide according to claim 1 and 2 is characterized in that: the high-temperature sterilization after the described fermentation is to sterilize 15 minutes under 121 ℃ ± 2 ℃ temperature.
5, the preparation method of zein active peptide according to claim 3 is characterized in that: the high-temperature sterilization after the described fermentation is to sterilize 15 minutes under 121 ℃ ± 2 ℃ temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100437096A CN1292673C (en) | 2004-07-14 | 2004-07-14 | Process for preparing active peptide of corn protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100437096A CN1292673C (en) | 2004-07-14 | 2004-07-14 | Process for preparing active peptide of corn protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1720813A true CN1720813A (en) | 2006-01-18 |
| CN1292673C CN1292673C (en) | 2007-01-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2004100437096A Expired - Fee Related CN1292673C (en) | 2004-07-14 | 2004-07-14 | Process for preparing active peptide of corn protein |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390564B (en) * | 2007-09-18 | 2011-01-26 | 中国食品发酵工业研究院 | Production method of corn separation protein |
| CN102191188A (en) * | 2010-03-10 | 2011-09-21 | 中国科学院福建物质结构研究所 | Method for culturing bacteria and application thereof |
| CN102715396A (en) * | 2012-07-17 | 2012-10-10 | 山东鲁健生物医药科技有限公司 | Special diet formula containing chitosan oligosaccharide and oligopeptide for hepatopaths |
| CN103555805A (en) * | 2013-11-19 | 2014-02-05 | 哈尔滨艾克尔食品科技有限公司 | Preparation method of wood frog protein peptide |
| CN109221601A (en) * | 2018-09-07 | 2019-01-18 | 南阳理工学院 | A kind of zein active peptide and its preparation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1021761C (en) * | 1991-08-01 | 1993-08-11 | 轻工业部食品发酵工业科学研究所 | Production method of feed complex enzyme-protein |
| CN1114352C (en) * | 2000-09-13 | 2003-07-16 | 山东大学 | Process for preparing nutritive activated peptide as additive |
-
2004
- 2004-07-14 CN CNB2004100437096A patent/CN1292673C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390564B (en) * | 2007-09-18 | 2011-01-26 | 中国食品发酵工业研究院 | Production method of corn separation protein |
| CN102191188A (en) * | 2010-03-10 | 2011-09-21 | 中国科学院福建物质结构研究所 | Method for culturing bacteria and application thereof |
| CN102715396A (en) * | 2012-07-17 | 2012-10-10 | 山东鲁健生物医药科技有限公司 | Special diet formula containing chitosan oligosaccharide and oligopeptide for hepatopaths |
| CN102715396B (en) * | 2012-07-17 | 2013-06-19 | 山东鲁健生物医药科技有限公司 | Special diet formula containing chitosan oligosaccharide and oligopeptide for hepatopaths |
| CN103555805A (en) * | 2013-11-19 | 2014-02-05 | 哈尔滨艾克尔食品科技有限公司 | Preparation method of wood frog protein peptide |
| CN109221601A (en) * | 2018-09-07 | 2019-01-18 | 南阳理工学院 | A kind of zein active peptide and its preparation |
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| Publication number | Publication date |
|---|---|
| CN1292673C (en) | 2007-01-03 |
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Granted publication date: 20070103 Termination date: 20110714 |