CN103555805A - Preparation method of wood frog protein peptide - Google Patents
Preparation method of wood frog protein peptide Download PDFInfo
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- CN103555805A CN103555805A CN201310577353.3A CN201310577353A CN103555805A CN 103555805 A CN103555805 A CN 103555805A CN 201310577353 A CN201310577353 A CN 201310577353A CN 103555805 A CN103555805 A CN 103555805A
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Abstract
The invention provides a preparation method of a wood frog protein peptide. The preparation method adopting artificially-bred wood frogs as materials comprises the following steps of: carrying out electric shock, and removing skin, internal organs, ovaries as well as genital glands; cleaning and cutting wood frog meat into blocks, blanching, cutting into pieces, and pressing into thin meat slices by a roller press; rubbing into shredded meat by a rubbing machine, drying until moisture content is less than 18%, and crushing to obtain wood frog meat powder; adding water to the wood frog meat powder for size mixing, adjusting the PH value, sterilizing, cooling, inoculating bacillus subtilis and fermenting; after the fermentation is ended, sterilizing, separating in a centrifugal manner, concentrating the liquid supernatant, and spray-drying to prepare the wood frog protein peptide. The preparation method disclosed by the invention is gentle in reaction and controlled easier; the final product is low in impurity content, free from foreign taste such as bitter and astringent taste, and lower in production cost.
Description
Technical field
The present invention relates to a kind of making method of protein, specifically a kind of making method of wood frog protein peptide.
Background technology
Wood frog, fine and tender taste, easily for human body is digested and assimilated, rich in proteins, also contains 18 seed amino acids, 9 kinds of VITAMIN, 13 kinds of trace elements, aspect medicinal, nourishing and beauty treatment, has very high using value,, the crowd, particularly valetudinarian and the old man that are applicable to the various ages are edible.
Modern scientific research shows, small-molecular peptides has higher biological activity and nutritive value than the amino acid of equivalent, peptide class and amino acid almost all can be absorbed and used by Epithelium of intestinal villus cell completely, enter into blood, then be transported to each position of organism, protein must just can be absorbed and used after digestion.Peptide class is the total free aminoacids faster than equivalent by organism absorption rate, greatly faster than the protein of equivalent.
Summary of the invention
The invention provides a kind of making method of wood frog protein peptide.
The present invention utilizes microbial fermentation technology that the protein transduction in wood frog meat is turned to protein peptide, in conversion process, can discharge the nitrogenous extracts such as the molten albumen of a large amount of flesh and amino acid, has increased its trophicity and functional.
The object of the present invention is achieved like this: take and propagate wood frog artificially as raw material, through shocking by electricity, remove the peel, go internal organ, removal ovary and sexual gland, clean, obtain wood frog meat, wood frog meat is cut into the cube meat of 5 cubic centimetres of left and right, in boiling water, blanching is 5 minutes, be cut into the sliced meat of 5-6 millimeter, the roll squeezer that is adjusted into 1 millimeter with gap is pressed into escalope again, and kneading machine is rubbed into shredded meat, shredded meat is extremely moisture below 18% through 55-60 ℃ of oven dry, through pulverizing to obtain wood frog digested tankage; Get wood frog digested tankage and add water and size mixing to material concentration 10-12%, adjust pH value to 6.5-7.0, through 121 ℃, sterilizing 30 minutes, be cooled to 30 ℃, press 8% access subtilis liquid seeds of slurries total amount, stir, under 28 ℃-30 ℃, rotating speed 180rpm condition, fermentation 48-60 hour; Fermentation ends, through 115 ℃, sterilizing 15 minutes, centrifugation, by supernatant liquor reconcentration to 25-30Bx, the dry wood frog protein peptide that makes of spraying.
The cultural method of described subtilis liquid seeds is: take 3g wood frog digested tankage, 4g glucose, 2g extractum carnis, 0.1g potassium primary phosphate, 0.05g sal epsom, add 90ml water, stir, 121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access subtilis inclined-plane seed, stirs, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours.
At present, the method that woods protein transduction is turned to protein peptide is mainly to have acid hydrolyzation and enzymolysis process.Acid hydrolyzation reaction is violent, and adds strong acid, High Temperature High Pressure, and reflection process and final product are all difficult to control, and can cause a large amount of amino acid whose destruction, and cause environmental pollution; Though enzyme process reaction conditions is gentleer, production cost is high, and the finished product bitter taste is obvious, and mouthfeel is poor.The present invention is that the method for fermenting by microorganism turns to protein peptide by the protein transduction in wood frog meat, its processing method reaction temperature and, than being easier to, control, the foreign matter content of its finished product is low, the peculiar smell such as nothing is bitter, puckery, compare with the method for enzymolysis process production protein peptide with acid hydrolyzation, the production cost of wood frog protein peptide of the present invention is lower.
Wood frog protein peptide of the present invention is that molecular weight is the oligopeptide of normal distribution between 500--2500 Dalton, main take the little peptide that formed by 3-6 amino acid as main, it does not need digestion after entering organism, but directly enter small intestine with the form of complete little peptide, be absorbed, and enter human recycle system, bring into play its function.The absorption rate of wood frog protein peptide will be much larger than the protein in wood frog meat.
Four, specific embodiment
First carry out liquid seeds cultivation, then carry out the preparation of wood frog protein peptide.
Embodiment 1
The cultivation of liquid seeds: take 6g wood frog digested tankage, 8g glucose, 4g extractum carnis, 0.2g potassium primary phosphate, 0.1g sal epsom, add 180ml water, stir, 121 ℃ of sterilizings 30 minutes, are cooled to 30 ℃, access subtilis inclined-plane seed, stir, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be subtilis liquid seeds.
The preparation of wood frog protein peptide: take and propagate wood frog artificially as raw material, through shocking by electricity, remove the peel, go internal organ, removal ovary and sexual gland, clean, obtain wood frog meat, wood frog meat is cut into the cube meat of 5 cubic centimetres of left and right, in boiling water, blanching is 5 minutes, be cut into the sliced meat of 5-6 millimeter, the roll squeezer that is adjusted into 1 millimeter with gap is pressed into escalope again, and kneading machine is rubbed into shredded meat, shredded meat is extremely moisture below 18% through 55-60 ℃ of oven dry, through pulverizing to obtain wood frog digested tankage; Get 120 crin frog digested tankages, add 880 grams of water of water, adjust pH value to 6.5-7.0, through 121 ℃, sterilizing 30 minutes, be cooled to 30 ℃, access 80 grams of subtilis liquid seeds, stir, under 28 ℃-30 ℃, rotating speed 180rpm condition, fermentation 48-60 hour; Fermentation ends, through 115 ℃, sterilizing 15 minutes, centrifugation, by supernatant liquor reconcentration to 25Bx, the dry wood frog protein peptide that makes of spraying.
Embodiment 2
The cultivation of liquid seeds: take 3g wood frog digested tankage, 4g glucose, 2g extractum carnis, 0.1g potassium primary phosphate, 0.05g sal epsom, add 90ml water, stir, 121 ℃ of sterilizings 30 minutes, are cooled to 30 ℃, access subtilis inclined-plane seed, stir, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be subtilis liquid seeds.
The preparation of wood frog protein peptide: take and propagate wood frog artificially as raw material, through shocking by electricity, remove the peel, go internal organ, removal ovary and sexual gland, clean, obtain wood frog meat, wood frog meat is cut into the cube meat of 5 cubic centimetres of left and right, in boiling water, blanching is 5 minutes, be cut into the sliced meat of 5-6 millimeter, the roll squeezer that is adjusted into 1 millimeter with gap is pressed into escalope again, and kneading machine is rubbed into shredded meat, shredded meat is extremely moisture below 18% through 55-60 ℃ of oven dry, through pulverizing to obtain wood frog digested tankage; Get 50 crin frog digested tankages, add 450 grams of water of water, adjust pH value to 6.5-7.0, through 121 ℃, sterilizing 30 minutes, be cooled to 30 ℃, access 40 grams of subtilis liquid seeds, stir, under 28 ℃-30 ℃, rotating speed 180rpm condition, fermentation 48-60 hour; Fermentation ends, through 115 ℃, sterilizing 15 minutes, centrifugation, by supernatant liquor reconcentration to 28Bx, the dry wood frog protein peptide that makes of spraying.
Claims (2)
1. the making method of a wood frog protein peptide, it is characterized in that: take and propagate wood frog artificially as raw material, through shocking by electricity, remove the peel, go internal organ, removal ovary and sexual gland, clean, obtain wood frog meat, wood frog meat is cut into the cube meat of 5 cubic centimetres of left and right, in boiling water, blanching is 5 minutes, then is cut into the sliced meat of 5-6 millimeter, the roll squeezer that is adjusted into 1 millimeter with gap is pressed into escalope, kneading machine is rubbed into shredded meat, and shredded meat is extremely moisture below 18% through 55-60 ℃ of oven dry, through pulverizing to obtain wood frog digested tankage; Get 120 crin frog digested tankages, add 880 grams of water of water, adjust pH value to 6.5-7.0, through 121 ℃, sterilizing 30 minutes, be cooled to 30 ℃, access 80 grams of subtilis liquid seeds, stir, under 28 ℃-30 ℃, rotating speed 180rpm condition, fermentation 48-60 hour; Fermentation ends, through 115 ℃, sterilizing 15 minutes, centrifugation, by supernatant liquor reconcentration to 25Bx, the dry wood frog protein peptide that makes of spraying.
2. the cultural method of subtilis liquid seeds claimed in claim 1 is: take 3g wood frog digested tankage, 4g glucose, 2g extractum carnis, 0.1g potassium primary phosphate, 0.05g sal epsom, add 90ml water, stir, 121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access subtilis inclined-plane seed, stirs, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310577353.3A CN103555805A (en) | 2013-11-19 | 2013-11-19 | Preparation method of wood frog protein peptide |
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| CN201310577353.3A CN103555805A (en) | 2013-11-19 | 2013-11-19 | Preparation method of wood frog protein peptide |
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| CN103555805A true CN103555805A (en) | 2014-02-05 |
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| CN201310577353.3A Pending CN103555805A (en) | 2013-11-19 | 2013-11-19 | Preparation method of wood frog protein peptide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106880777A (en) * | 2017-02-27 | 2017-06-23 | 武文海 | A kind of Helth-care product with functions of prolonging life and nourishing face and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1720813A (en) * | 2004-07-14 | 2006-01-18 | 哈尔滨中冠生物工程有限公司 | Process for preparing active peptide of corn protein |
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2013
- 2013-11-19 CN CN201310577353.3A patent/CN103555805A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1720813A (en) * | 2004-07-14 | 2006-01-18 | 哈尔滨中冠生物工程有限公司 | Process for preparing active peptide of corn protein |
Non-Patent Citations (1)
| Title |
|---|
| 孔繁东 等: "微生物酶水解林蛙肉蛋白质及产物抗氧化活性初探", 《中国酿造》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106880777A (en) * | 2017-02-27 | 2017-06-23 | 武文海 | A kind of Helth-care product with functions of prolonging life and nourishing face and preparation method thereof |
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Application publication date: 20140205 |