CN1021761C - Production method of feed complex enzyme-protein - Google Patents

Production method of feed complex enzyme-protein Download PDF

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Publication number
CN1021761C
CN1021761C CN91105141A CN91105141A CN1021761C CN 1021761 C CN1021761 C CN 1021761C CN 91105141 A CN91105141 A CN 91105141A CN 91105141 A CN91105141 A CN 91105141A CN 1021761 C CN1021761 C CN 1021761C
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China
Prior art keywords
protein
production method
complex enzyme
feed
corn
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Expired - Fee Related
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CN91105141A
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Chinese (zh)
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CN1059260A (en
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蔡木易
王定昌
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INST OF FOOD LEAVEN INDUSTRY AND SCIENCE MINISTRY OF LIGHT INDUSTRY
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INST OF FOOD LEAVEN INDUSTRY AND SCIENCE MINISTRY OF LIGHT INDUSTRY
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Priority to CN91105141A priority Critical patent/CN1021761C/en
Publication of CN1059260A publication Critical patent/CN1059260A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a production method of feed complex enzyme-protein. Various raw materials are pulverized by a hammer type pulverizer and proportionally mixed, and then the raw materials are uniformly mixed by adding water. The raw materials are sterilized and cooled, and solid microorganism is inoculated to make the raw materials fermented by mixed culture. After being fermented, the raw materials are dried at a low temperature and pulverized to obtain finished product. The product comprises active complex enzyme and growth factors requisite for animals, and the feed complex enzyme-protein produced by the method has high protein net increased value. Besides, feed added with the feed complex enzyme-protein has rich and balanced nutrients, and high feed reward, and the feed complex enzyme-protein can promote digestion. The method is also suitable for processing waste dregs produced in other agricultural processing industries using corn as raw material, such as waste dregs produced in the production processes of corn alcohol and corn high fructose syrup.

Description

Production method of feed complex enzyme-protein
The present invention relates to a kind of multiple byproduct that produces in the cornstarch production process and production method of the byproduct production feed complex enzyme-albumen in the plant process utilized.
At present, the protein shortage is a key factor of puzzlement feedstuff industry development.How existing protein resource being carried out degree of depth utilization, improve the price of deed, is an important topic of feedstuff industry.Around this content, the parties concerned has carried out very big effort.Power is sought on the way by way of solving this difficult problem.The method of having used at present has: (1) chemical method; Be hydrolyzed to handle as the raw material that feather, human hair etc. is contained keratoprotein and obtain protein hydrolysate and amino acid.This method investment is big, cost is higher.(2) physics method; Expanded as some feedstuff is pressurizeed, the internal structure of change raw material makes it to be easy to be assimilated by poultry.Because this method only changes raw material mix, composition is constant, so the price of deed is not too high.(3) bioanalysis; Be material to be carried out biochemical treatment, the structure of raw material and composition aspect are got a new look by former.This method cycle is long, the cost height.
How improve in the research of the price of deed, adopt plus enzyme abroad, be about to the finished product enzyme and add in the feed, obtain good effect, utilize plus enzyme to improve the utilization ratio of cereal in monogastric animal feeds such as pig, chicken as Canada.The U.S. adds enzyme preparation to contain feeds the chicken weightening finish obviously in the higher barley diet of crude fibre, and can reduce food consumption.The result of study of the Soviet Union has also confirmed above-mentioned conclusion.Domestic had Preliminary study to the Application and Development of feed enzyme preparation in recent years, obtained some progress, wraps up in Fan city biochemical research as Hubei complex enzyme formulation is added in the feed, obtains significantly result.Said method just directly adds the finished product enzyme in the feed simply, and various raw materials in the feed itself be there is no more influences.The main cause that influences the plant protein fodder remuneration in animal husbandry is the unbalanced of the contained nutrition component of certain plants albumen, and lack the unknown factor of the growth of animal that had in animal protein and the single cell protein, in corn protein, tryptophan and lysine content deficiency, the dregs of beans methionine content is less, and carotene and vitamin D content are less.
The purpose of this invention is to provide one and utilize the multiple accessory substance of producing in the cornstarch production, the leftover bits and pieces that the vegetable oil process produces is a primary raw material, by the fermentation of solid microbe mixed culture, produce the feed protein processing method of providing needed active complex enzyme composition in the animal metabolism process.
Purpose of the present invention is realized by following technical proposal.
With various raw materials, as corn lees, corn germ cake, corn protein powder, dregs of beans, mix in proportion after being pulverized by beater grinder, add the water mixing, through sterilization, cooling, insert that the solid microbe mixed culture ferments, after the fermentation ends by low temperature drying, get product after pulverizing.The present invention is by carrying out fermentation process to the raw material that contains vegetable protein, make and contain the required active complex enzyme of animal production in the finished product, improved the utilization rate of feed, and make in the product except that vegetable protein, increased the part mycoprotein, improved the protein content of raw material, improved the nutrition in the raw material, in addition owing to containing the effect that active complex enzyme composition that fermentation produces and growth factor have the feed digestive aid in this product.
The present invention compared with prior art, cost is low, technology is simple, the cycle is short, protein net added value height, feed nutrition abundant, balanced, price of deed height, and can promote digestion.The non-medicine of this product, without any side effects.
The invention will be further described below in conjunction with embodiment.
Percentage that the application is used and ratio are removed and are specialized all by weight.
At first being pulverized various raw materials by beater grinder after, the present invention mixes in proportion, add the water mixing, various material components and ratio range are: corn lees is 10-20%, corn germ cake is 10-20%, dregs of beans is 20-30%, corn protein powder is 30-40%, and it is 1 that its water addition ratio is calculated by butt: 1-1.2.Through sterilization, cooling, its sterilising temp is 100 ℃ then, and sterilization time is 30 minutes, and after will cultivating ripe solid microbe culture more respectively and mixing, the inoculum concentration by 1% inserts in the mixed material of sterilization back cooling, mixes thoroughly with mechanical means.Insert in the solid biologic reactor and cultivate, its condition of culture is as follows, and temperature is 33 ℃ ± 2, and the time is 40 hours, and ventilation is that material per ton per hour ventilates 8000 cubic metres.After cultivating end, through pneumatic conveying drying, baking temperature is 75 ℃ with material, must not surpass 80 ℃, and the final moisture content of product is below 8%, and smashing fineness is the 60-80 order.
Said solid microbe mixture is formed through enlarging to cultivate by following bacterial classification:
Aspergillus niger Aspergillus niger
Aspergillus oryzae Aspergillus orgzae
Endomycopsis Fibnligera Endonycopsis fibuligera
Aspergillus awamori Aspergillus awamari
Aspergillus terricola Aspergillus terricola
Geotrichum candidum Geotrichum cadidum
Formosan Rhizopus Rhizopus formosensis
The present invention is all having greatly improved material component by fermentation aspect amino acid content and other nutrition component.Be component situation of change before and after the fermentation below.
It is the processing of other agricultural basic processing industry waste residue of raw material that the present invention is equally applicable to the corn, the waste residue that produces in producing as corn alcohol, corn high fructose syrup.By utilization, improve the protein content and the price of deed, so the present invention has suitable application prospects to these waste residues.
Finished product after composition unit's fermenting raw materials
Protein % 43.5 51.3
Ash % 1.8 1.8
Moisture content % 3.5 4.5
Fat % 4.82 3.32
Crude fibre % 5.14 9.2
Total reducing sugar % 3.04 2.87
Reduced sugar % 2.32 2.87
V AIu/100g 222 107
V B1mg/100g 0.06 0.11
V B2mg/100g 0.52 0.62
V Cmg/100g 6.6 6.19
V DMg/100g trace trace
V Emg/100g 0.015 7.98
V Kμg/100g 1.60 2.84
Plumbous PPm 4.68 6.5
Manganese PPm 9.90 21.1
Phosphorus PPm 1346.90 2515.0
Potassium PPm 2771.20 4121.2
Sodium PPm 327.20 436.7
Calcium PPm 371.50 551.3
Magnesium PPm 798.30 1342.8
Copper PPm 11.70 4.9
Zinc PPm 20.00 21.0
Iron PPm 351.40 373.4
Finished product after composition unit's fermenting raw materials
Selenium PPm 0.037 0.038
Asparatate g/100g 2.67 3.37
Threonine g/100g 1.47 1.81
Serine g/100g 2.22 2.58
Glutamic acid g/100g 9.68 9.34
Glycine g/100g 1.20 1.47
Alanine g/100g 4.02 4.13
Cystine g/100g 0.64 0.68
Valine g/100g 2.17 2.45
Methionine g/100g 0.82 0.91
Isoleucine g/100g 1.90 2.17
Leucine g/100g 7.50 7.09
Tyrosine g/100g 1.86 1.95
Phenylalanine g/100g 2.73 2.89
Lysine g/100g 0.93 1.18
Ammonia g/100g 0.64 0.72
Histidine g/100g 0.83 0.91
Arginine g/100g 2.01 2.39
Proline g/100g 4.04 3.70
Tryptophan g/100g 0.14 0.29
Total amino acid content g/100g 47.1 50.6
AFB μ g/kg does not detect
Pathogenic bacteria-
Staphylococcus aureus does not detect
Salmonella does not detect

Claims (5)

1, a kind of feed complex enzyme-protein production method, it is characterized in that will be by the accessory substance in the cornstarch production and produce the raw material pulverizing that the slag dregs of rice that vegetable oil produces form after mix, add the water mixing, after sterilization, cooling, insert by following bacterial classification: a. aspergillus niger Aspergillus niger; B. aspergillus oryzae Aspergillus orgzae; C. Endomycopsis Fibnligera Endonycopsis fibuligera; D. aspergillus awamori Asperhillus awamari; E. aspergillus terricola Aspergillus terricola; F. geotrichum candidum Geotrichum cadidum; G. the mixed culture formed of Formosan Rhizopus Rhizopus formosensis ferments, and by being no more than 80 ℃ low temperature drying, gets product after the pulverizing after the fermentation ends.
2, production method according to claim 1 is characterized in that said raw material (weight %) composed as follows:
Corn lees is 10-20%;
Corn germ cake is 10-20%;
Dregs of beans is 20-30%;
Corn protein powder is 30-40%;
Water addition ratio is pressed butt weight and is calculated 1: 1-1.2.
3, production method according to claim 1 and 2, the sterilising temp that it is characterized in that raw material is 100 ℃, sterilization time is 30 minutes.
4, production method according to claim 1 is characterized in that the main body Zymolysis Equipment in this production method adopts mechanical ventilation type solid biochemical reactor, and fermentation temperature is 30 ℃ of fermentation periods 40 hours.
5, production method according to claim 1 is characterized in that drying process adopts low temperature drying technology in this production method, and baking temperature is 75 ℃, keeps the complex enzyme activity simultaneously again at sterilizing-drying.
CN91105141A 1991-08-01 1991-08-01 Production method of feed complex enzyme-protein Expired - Fee Related CN1021761C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN91105141A CN1021761C (en) 1991-08-01 1991-08-01 Production method of feed complex enzyme-protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN91105141A CN1021761C (en) 1991-08-01 1991-08-01 Production method of feed complex enzyme-protein

Publications (2)

Publication Number Publication Date
CN1059260A CN1059260A (en) 1992-03-11
CN1021761C true CN1021761C (en) 1993-08-11

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Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1036891C (en) * 1993-06-03 1998-01-07 山东大学 Method for making basic feed for rabbits
CN1292673C (en) * 2004-07-14 2007-01-03 哈尔滨中冠生物工程有限公司 Process for preparing active peptide of corn protein
CN101427782B (en) * 2008-11-27 2012-05-30 赵敏 Bifiobacterium beverage of corn and preparation method thereof
CN102210388B (en) * 2011-07-27 2012-07-04 河北联合大学 Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction
CN102406066B (en) * 2011-10-11 2013-01-16 中北大学 Feed additive for dairy cow and preparation method thereof
CN102406060B (en) * 2011-10-11 2013-06-12 中北大学 Feed additive and production method thereof
CN103652327B (en) * 2013-12-10 2015-04-01 青岛琅琊台集团股份有限公司 Biological feed produced by wastes
CN104621343A (en) * 2014-12-25 2015-05-20 山东西王糖业有限公司 Production method of liquid feed
CN106376724A (en) * 2016-08-30 2017-02-08 惠州市瑰拉科技有限公司 Corn meal fermentation processing method

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