CN1171612C - Mixed Chinese medicine prepn. for treating natural miscrarriage - Google Patents
Mixed Chinese medicine prepn. for treating natural miscrarriage Download PDFInfo
- Publication number
- CN1171612C CN1171612C CNB02111241XA CN02111241A CN1171612C CN 1171612 C CN1171612 C CN 1171612C CN B02111241X A CNB02111241X A CN B02111241XA CN 02111241 A CN02111241 A CN 02111241A CN 1171612 C CN1171612 C CN 1171612C
- Authority
- CN
- China
- Prior art keywords
- radix
- medicine
- present
- rhizoma atractylodis
- spontaneous abortion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003814 drug Substances 0.000 title claims abstract description 33
- 206010000234 Abortion spontaneous Diseases 0.000 claims abstract description 29
- 208000000995 spontaneous abortion Diseases 0.000 claims abstract description 29
- 238000002360 preparation method Methods 0.000 claims abstract description 25
- 235000004347 Perilla Nutrition 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract 2
- 229940079593 drug Drugs 0.000 claims description 18
- 241000756943 Codonopsis Species 0.000 claims description 13
- 210000000582 semen Anatomy 0.000 claims description 11
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 241000229722 Perilla <angiosperm> Species 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000002552 dosage form Substances 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000003921 oil Substances 0.000 claims description 3
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 239000000341 volatile oil Substances 0.000 claims description 3
- 239000000047 product Substances 0.000 claims 1
- 238000010298 pulverizing process Methods 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 12
- 206010000210 abortion Diseases 0.000 abstract description 11
- 231100000176 abortion Toxicity 0.000 abstract description 11
- 210000003734 kidney Anatomy 0.000 abstract description 10
- 230000002124 endocrine Effects 0.000 abstract description 6
- 229940088597 hormone Drugs 0.000 abstract description 5
- 239000005556 hormone Substances 0.000 abstract description 5
- 230000036039 immunity Effects 0.000 abstract description 5
- 230000007812 deficiency Effects 0.000 abstract description 3
- 230000008506 pathogenesis Effects 0.000 abstract description 3
- 230000001105 regulatory effect Effects 0.000 abstract description 3
- 208000032023 Signs and Symptoms Diseases 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 abstract description 2
- 230000004069 differentiation Effects 0.000 abstract description 2
- 230000001681 protective effect Effects 0.000 abstract description 2
- 241000132012 Atractylodes Species 0.000 abstract 1
- 241000050051 Chelone glabra Species 0.000 abstract 1
- 240000007371 Cuscuta campestris Species 0.000 abstract 1
- 241000208688 Eucommia Species 0.000 abstract 1
- 244000236658 Paeonia lactiflora Species 0.000 abstract 1
- 235000008598 Paeonia lactiflora Nutrition 0.000 abstract 1
- 244000124853 Perilla frutescens Species 0.000 abstract 1
- 241000316342 Taxillus Species 0.000 abstract 1
- 230000002159 abnormal effect Effects 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000003832 immune regulation Effects 0.000 abstract 1
- 208000015994 miscarriage Diseases 0.000 description 13
- 230000035935 pregnancy Effects 0.000 description 11
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 230000000903 blocking effect Effects 0.000 description 9
- OZVBMTJYIDMWIL-AYFBDAFISA-N bromocriptine Chemical compound C1=CC(C=2[C@H](N(C)C[C@@H](C=2)C(=O)N[C@]2(C(=O)N3[C@H](C(N4CCC[C@H]4[C@]3(O)O2)=O)CC(C)C)C(C)C)C2)=C3C2=C(Br)NC3=C1 OZVBMTJYIDMWIL-AYFBDAFISA-N 0.000 description 7
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 4
- 229960002802 bromocriptine Drugs 0.000 description 4
- 210000003754 fetus Anatomy 0.000 description 4
- 239000003390 Chinese drug Substances 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 239000007952 growth promoter Substances 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 230000008774 maternal effect Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 2
- 102100037850 Interferon gamma Human genes 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000207929 Scutellaria Species 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000007368 endocrine function Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000004957 immunoregulator effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000007799 mixed lymphocyte reaction assay Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 238000011552 rat model Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000007086 side reaction Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000010254 subcutaneous injection Methods 0.000 description 2
- 239000007929 subcutaneous injection Substances 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- 206010000242 Abortion threatened Diseases 0.000 description 1
- 208000031648 Body Weight Changes Diseases 0.000 description 1
- 101710112752 Cytotoxin Proteins 0.000 description 1
- 241000721047 Danaus plexippus Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 208000005985 Threatened Abortion Diseases 0.000 description 1
- 208000003441 Transfusion reaction Diseases 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000003302 anti-idiotype Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000004579 body weight change Effects 0.000 description 1
- 210000003679 cervix uteri Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 230000004540 complement-dependent cytotoxicity Effects 0.000 description 1
- 210000004246 corpus luteum Anatomy 0.000 description 1
- 239000000431 corpus luteum hormone Substances 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 239000002619 cytotoxin Substances 0.000 description 1
- 210000003785 decidua Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000008482 dysregulation Effects 0.000 description 1
- 230000032692 embryo implantation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000001456 gonadotroph Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 229940088592 immunologic factor Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000682 maximum tolerated dose Toxicity 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention belongs to the field of pharmacy, which more specifically relates to a compound Chinese medicinal preparation for treating abortion caused by abnormal immune regulation and/or incretion function deficiency. The present invention uses pilose asiabell root, dodder seed, Chinese taxillus twig, eucommia bark, white atractylodes rhizome, white peony root, baical skullcap root, perilla stem, etc. as raw medicinal materials to prepare the compound preparation for treating spontaneous abortion according to the Chinese medicine theories of 'etiology and pathogenesis', 'determining the treatment based on differentiation of symptoms and signs' and 'kidney controlling reproduction'. The successful rate of tocolysis can reach 90%. The present invention performs the tocolysis function by regulating the protective immunity function of foetuses and improving the level of endocrine hormone, and is beneficial to the growth and development of the foetuses. The present invention can be applied to all stages of gestational periods, and can be taken for a long time without obvious side effect.
Description
Technical field
The invention belongs to pharmaceutical field, be specifically related to a kind of because of the not enough institute of immunoregulatory abnormality and/or endocrine function to the compound Chinese medicinal preparation of miscarrying.
Background technology
Relevant spontaneous abortion is a common gynecological disease, frequently-occurring disease, and cause of disease complexity, modal is that immune factor, endocrine factors, infective agent, cervix uteri insufficiency, uterus pathological changes, inherited genetic factors etc. cause.The maternal immunity normal regulating plays very main effect in keeping gestation, and the maternal immunity dysregulation be cause the miscarriage most commonly encountered diseases because of, with merge existing with cryptorrhea often.There is 90% cause of disease relevant approximately, more seriously influenced women's physical and mental health, so become the current focus and the difficult point of research both at home and abroad with female tire immunoregulatory abnormality and/or endocrine function deficiency.The spontaneous abortion that causes because of female tire immunologic hypofunction clinically, doctor trained in Western medicine often adopts the blood transfusion therapy, and the success rate of preventing miscarriage is about 80%, but this detection must possess the appointed condition that detects female blood blocking antibody and blood transfusion, and blood transfusion also may cause transfusion reaction, so can not be widely used.
Summary of the invention
The purpose of this invention is to provide a kind of compound Chinese medicinal preparation for the treatment of spontaneous abortion, its curative effect height, no side reaction, and help the growth promoter of fetus.
The present invention thinks that according to pathogen and pathology of tcm, determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs " kidney governing reproduction " theory the pathogenesis of above-mentioned spontaneous abortion is the liability to abortion of suffering from a deficiency of the kidney, adopts and tonifies Qi of the kidney to curing mainly then control, and be monarch drug with Radix Codonopsis invigorating the spleen and replenishing QI, nourishing blood to promote the production of body fluid; Semen Cuscutae the kidney invigorating tonifying YIN, sperm adding and marrow healthing, Herba Taxilli liver and kidney tonifying, nourishing blood and preventing abortion, Cortex Eucommiae liver and kidney tonifying, antiabortive leak-stopping are ministerial drug; Rhizoma Atractylodis Macrocephalae air making-up and spleen enlivening is antiabortive, the Radix Paeoniae Alba yin fluid astringing easing the affected liver to relieve pain that nourishes blood, and the Radix Scutellariae clearing away heat to stop bleeding is antiabortive to be adjuvant drug; The sharp diaphragm of Caulis Perillae the chest stuffiness relieving, pleasant antiabortive, tonify without causing stagnation is a messenger drug.All medicines share spleen invigorating fortune and not dry, nourish blood and do not grow greasyly partially, inspire kidney qi and do not have dry fraud just, and this compound preparation is played altogether and tonified Qi of the kidney, the merit of clearing heat and preventing abortion.Adopt the success of preventing miscarriage of this compound Chinese medicinal preparation treatment spontaneous abortion to demonstrate its special advantages and definite curative effect.Through clinical treatment thousands of examples of miscarrying, the blocking antibody level that the winner that prevents miscarriage after the treatment is low obviously rises, and follows pregnant endocrine hormone level to raise.Confirm the present invention by adjusting female tire protective immunity function and improving the endocrine hormone level and play anti-abortional effect, the success rate of preventing miscarriage reaches 90%.Follow up a case by regular visits to the success filial generation of preventing miscarriage, its heredity is grown, and intelligence is all normal, confirms that the present invention also can be applicable to trimester of pregnancy in any stage, anti-abortional effect is not only arranged, and help the growth promoter of fetus.Taking no obvious side reaction for a long time takes place.
Compound preparation of the present invention is made by following component: (consumption is a weight portion)
Radix Codonopsis 8-30 Semen Cuscutae 6-16 Herba Taxilli 6-16
Cortex Eucommiae 6-16 Rhizoma Atractylodis Macrocephalae 3-18 Radix Paeoniae Alba 4-15
The formula optimization weight proportion scope that Radix Scutellariae 10-30 Caulis Perillae 3-12 prepares compound preparation of the present invention is
Radix Codonopsis 10-20 Semen Cuscutae 8-14 Herba Taxilli 8-14
Cortex Eucommiae 8-14 Rhizoma Atractylodis Macrocephalae 5-15 Radix Paeoniae Alba 6-12
The optimum weight proportioning of Radix Scutellariae 10-20 Caulis Perillae 5-10 compound preparation of the present invention is
Radix Codonopsis 15 gram Semen Cuscutae 12 gram Herba Taxillis 12 grams
The Cortex Eucommiae 12 gram Rhizoma Atractylodis Macrocephalaes 6 gram Radix Paeoniae Albas 9 grams
The preparation method that Radix Scutellariae 15 gram Caulis Perillaes 6 grams are made compound preparation of the present invention with above-mentioned each component is:
Above-mentioned eight flavor Chinese medicine crude drugs are added the decocting 2 hours of 5 times of amounts, filter.Filtering residue adds 4 times of amount ordinary waters to be fried in shallow oil 2 hours, filtered.Twice filtrate merges, and places sedimentation more than 12 hours, gets supernatant and is evaporated to extractum below 60 ℃ and contains crude drug 2g/ml.Extractum is put in the stainless steel disc, and vacuum drying (below 60 ℃) becomes dried cream (extractum rate about 15%) to pulverize, and adds an amount of excipient, makes 40 order granule kidney-nourishing tcm drug powder and gets compound preparation of the present invention.Every bag 15 grams.Take after mixing it with water with 200 milliliters of boiled water, be clarification light coffee color solution.
At the enterprising one-step optimization in above-mentioned preparation method basis, at first extract the volatile oil component of Caulis Perillae, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, all the other crude drugs are then by former method preparation, and two kinds of compositions mixing are taken then, and its curative effect is better.Make solvent with distilled water and with the volatile ingredient that powder is complementary during use, make the 0.5g/ml medicinal liquid, contain crude drug 2.25g/ml.
Dosage form and specification: electuary, every pair of Chinese medicine is made 2 bag electuaries, every bag 15g.
Usage and dosage: obey 1 bag at every turn, obey every day 2 times.
The course of treatment: be 1 course of treatment January, generally obeys the 1-2 course of treatment, and pact is to pregnant March or exceed the pregnant week of in the past miscarrying.
Preparation of the present invention can be made said dosage form on any pharmaceutics.
The present invention through the animal acute toxicity test result is: the mouse stomach preliminary experiment can not surveyed LD
50, measure maximum tolerated dose＞20g extractum/kg body weight (being equivalent to 68.5 g crude drug/kg body weight).Behind the administration 20g/kg, mice is movable normal, does not see that other is unusual.Mice was movable normal, all right in second day, observed no animal dead continuously seven days.The mice body weight change is normal.
For showing the definite effect of compound preparation of the present invention to the treatment spontaneous abortion, the present invention causes the miscarriage rat model to Ergolactin and tests, the result shows that the improvement bromocriptine causes the low PRL of early abortion mouse model performance, low P and the imbalance of Th1/Th2 cytokine balance, be partial to the Th1 type, depart from the Th2 type, abortion ratio obviously raises, litter size obviously reduces, and has the feature of endocrine-immune disorder.Tonify Qi of the kidney and prevent miscarriage after the compound Chinese medicinal preparation and feed the present invention, pregnancy associated hormone PRL, P obviously rise, adjust decidua Th1/Th2 cytokine balance, deflection Th2 type reduces abortion ratio, the gestation success rate obviously rises to 75%-83%, litter size, average uterus weight obviously increase, and statistics has significant difference.Show that medicine of the present invention can provide good microenvironment for blastocyst implantation by the regulating and controlling effect to female tire interface endocrine immunological network, keep gestation, realize its anti-abortional effect.
Table 1 is the influence of various dose bromocriptine to rat pregnancy outcome.
Table 1
Group 1mg/d 0.25mg/d 0.125mg/d
N abortion ratio n abortion ratio n litter size abortion ratio
Chinese drug-treated group 9 100% 10 100% 15 114
★ ★27%
★
Bromocriptine group 12 100% 6 100% 12 25 75%
Annotate: two groups of abortion ratio difference have statistical significance,
*P＜0.05; Two groups of litter size difference
Obvious statistical significance is arranged,
★ ★P＜0.01
The bromocriptine group was in pregnant 6,7,8 days subcutaneous injection bromocriptines in the table, and dosage successively decreases according to multiple from 1mg/d; Chinese drug-treated group was in pregnant 6,7,8 days subcutaneous injection bromocriptines, and dosage successively decreases according to multiple from 1mg/d, and gavaged Chinese medicine in pregnant 1-12 days.
Table 2, table 3 are Chinese medicine of the present invention causes the miscarriage excretory adjusting of rat model PRL and female tire interface Th cytokine to bromocriptine influences.
Table 2
Gestation natural law A group (n=27) B group (n=24) C group (n=12) D group (n=23) E group (n=24) F group (n=24)
D4 9014±3.054Δ 1.0672±2?451 9.183±0.553Δ 9193±1.683Δ 9.585±1.809Δ
D7 15.678±7.473 9.967±6.170
* 18.298±0.882 17.315±2.110 17.487±2000
D10 16.388±7.147 8.520±7.l86
* 19.351±0.831 1.9.003±1.964 1.9.957±3179
D12 23.653±10.319 12.314±11.989
*?26.421±0.620 30.459±4.131 29.196±2.387
2.935±1.207
**
*A, C, D, E organize than P＜0.05 with pregnant same period,
*Respectively organize different pregnancy phases relatively with A-E
P＜0.05, Δ and A, C, D, E organize different pregnant day than P＜0.01,
A group in the table: Chinese drug-treated group, B group: bromocriptine group, C group: PRL group, D group: ethanol group, E group: distilled water group, F group: rutting period.
Table 3
Group n TNF-α IFN-γ IL-4 IL-10 IL-10/IFN-γ
A 14 27.52±6.16 17.20±1.13 64.81±307 46.56±3.83 272±0.29
* * * * *
B 12 51.19±3.56 21.54±2.16 22.21±2.42 22.40±2.50 105±0.13
D 10 15.84±2.56 16.38±1.39 51.44±2.04 48.84±5.02 2.98±0.45
E 9 14.83±2.41 16.66±1.04 54.20±1.55 52.24±3.43 3.13±0.14
F 12 22.38±3.51Δ 14.04±3.02 30.22±5.87Δ 20.87±1.61Δ 1.53±0.26Δ
*Compare P＜0.05 with A, D, E group; Δ and D, E organize relatively
▲P＜0.05
In order to confirm the treatment by Chinese herbs spontaneous abortion to unusual female tire immunity and endocrine influence, the present invention treats spontaneous abortion 108 examples, wherein threatened abortion 40 examples; Recurrent spontaneous abortion 68 examples, the success rate 90.74% of preventing miscarriage, patient's peripheral blood blocking antibodies changed before and after wherein 92 examples were observed treatment, comprise sealing efficient (Blocking Effect, BE): adopt unidirectional mixed lymphocyte reaction blocking test, claim MLR-BE again; Anti-idiotype antibody (AntiidiotypeAntibody, AIA): adopt unidirectional mixed lymphocyte reaction sealing inhibition test; Cytotoxic antibody (Cytotoxin Antibody, CTA): adopt complement-dependent cytotoxicity test.Put the method for exempting from simultaneously and survey peripheral blood chorion gonadotrophic hormone beta subunit (β-hCG), progesterone (P), estradiol (E
2) serial dynamic change, the periodic review B ultrasonic is followed up a case by regular visits to the fetal development situation.92 routine patient B E, AIA, CTX lack the unusual incidence rate 93.48% of 1-3 item index, prevent miscarriage after the treatment winner's blocking antibody BE, AIA, the CTX level (P＜0.05-0.01) that obviously rises; Blood β-hCG, P, E
2Carrying out property of level is increased within the maternal physiology in pregnancy scope.The failure Aborter that prevents miscarriage, low blocking antibody does not rise, and normal blocking antibody obviously descends blood β-hCG, P, E
2Level descends.Presentation of results the present invention strengthens the fetus immunoprotection by improving parent blocking antibody level, impels corpus luteum of pregnancy, Placenta Hominis to grow, and secretes enough horizontal corpus luteum hormone, plays the therapeutical effect of preventing miscarriage.Compound preparation determined curative effect of the present invention can be used for trimester of pregnancy in any stage, and anti-abortional effect is not only arranged, and helps the growth promoter of fetus, can be widely used in clinical.
The specific embodiment
Embodiment 1
Take by weighing raw material (kilogram) by following proportioning
Radix Codonopsis 15 Semen Cuscutae 12 Herba Taxillis 12 Cortexs Eucommiae 12
The Rhizoma Atractylodis Macrocephalae 6 Radix Paeoniae Albas 9 Radix Scutellariaes 15 Caulis Perillaes 6
Above-mentioned eight flavor Chinese medicine crude drugs are added the decocting 2 hours of 5 times of amounts, filter.Filtering residue adds 4 times of amount ordinary waters to be fried in shallow oil 2 hours, filtered.Twice filtrate merges, and places sedimentation more than 12 hours, gets supernatant and is evaporated to extractum below 60 ℃ and contains crude drug 2g/ml.Extractum is put in the stainless steel disc, and vacuum drying (below 60 ℃) becomes dried cream (extractum rate about 15%) to pulverize, and adds an amount of excipient, makes 40 order granule kidney-nourishing tcm drug powder and gets compound preparation of the present invention.Every bag 15 grams of packing.
Embodiment 2
Take by weighing raw material (kilogram) by following proportioning
Radix Codonopsis 15 Semen Cuscutae 12 Herba Taxillis 12 Cortexs Eucommiae 12
The Rhizoma Atractylodis Macrocephalae 6 Radix Paeoniae Albas 9 Radix Scutellariaes 15 Caulis Perillaes 6
At first extract the volatile oil component of Caulis Perillae, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, all the other raw material crude drugs are then pressed the preparation of embodiment 1 method, and two kinds of compositions mixing are taken then, and its curative effect is better.Make solvent with distilled water and with the volatile ingredient that powder is complementary during use, make the 0.5g/ml medicinal liquid, contain crude drug 2.25g/ml.
Claims (6)
1, a kind of medicine for the treatment of spontaneous abortion is characterized in that it being to be made by following materials of weight proportions:
Radix Codonopsis 8-30 Semen Cuscutae 6-16 Herba Taxilli 6-16
Cortex Eucommiae 6-16 Rhizoma Atractylodis Macrocephalae 3-12 Radix Paeoniae Alba 4-15
Radix Scutellariae 10-30 Caulis Perillae 3-12.
2. by the medicine of the described treatment spontaneous abortion of claim 1, wherein the weight proportion of each raw material is:
Radix Codonopsis 10-20 Semen Cuscutae 8-14 Herba Taxilli 8-14
Cortex Eucommiae 8-14 Rhizoma Atractylodis Macrocephalae 5-10 Radix Paeoniae Alba 6-12
Radix Scutellariae 10-20 Caulis Perillae 5-10.
3. by the medicine of the described treatment spontaneous abortion of claim 1, wherein the weight proportion of each raw material is:
Radix Codonopsis 15 Semen Cuscutae 12 Herba Taxillis 12
The Cortex Eucommiae 12 Rhizoma Atractylodis Macrocephalaes 6 Radix Paeoniae Albas 9
Radix Scutellariae 15 Caulis Perillaes 6.
4. by the medicine of the described treatment spontaneous abortion of claim 1, it is characterized in that described pharmaceutical dosage form is extractum or dry powder doses.
5. the preparation method of the medicine of the treatment spontaneous abortion of claim 1 is characterized in that being undertaken by following step:
1) Chinese medicine crude drug Radix Codonopsis, Semen Cuscutae, Herba Taxilli, the Cortex Eucommiae, the Rhizoma Atractylodis Macrocephalae, the Radix Paeoniae Alba, Radix Scutellariae and Caulis Perillae are added the decocting 2 hours of 5 times of amounts, filter;
2) filtering residue adds 4 times of water gagings and fried in shallow oil 2 hours, filters;
3) twice filtrate merges, and places sedimentation more than 12 hours, gets supernatant and is evaporated to extractum below 60 ℃;
4) the extractum vacuum drying becomes dried cream, pulverizing, adds excipient, makes 40 order granule powder.
6, by the method for claim 5, it is characterized in that
1) elder generation extracts the volatile oil component of Caulis Perillae, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae;
2) all the other crude drugs of Semen Cuscutae, Herba Taxilli, the Cortex Eucommiae, the Radix Paeoniae Alba and Radix Scutellariae are by the method preparation of claim 5;
3) above-mentioned 1) and 2) gained two parts product is solvent with the distilled water, makes liquid preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02111241XA CN1171612C (en) | 2002-04-02 | 2002-04-02 | Mixed Chinese medicine prepn. for treating natural miscrarriage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02111241XA CN1171612C (en) | 2002-04-02 | 2002-04-02 | Mixed Chinese medicine prepn. for treating natural miscrarriage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1375322A CN1375322A (en) | 2002-10-23 |
| CN1171612C true CN1171612C (en) | 2004-10-20 |
Family
ID=4741466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB02111241XA Expired - Lifetime CN1171612C (en) | 2002-04-02 | 2002-04-02 | Mixed Chinese medicine prepn. for treating natural miscrarriage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1171612C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103463365A (en) * | 2013-09-12 | 2013-12-25 | 洪洞县浩天农业开发有限公司 | Medicament for treating ewe habitual abortion |
| CN104189642A (en) * | 2014-09-05 | 2014-12-10 | 扬中牧乐药业有限公司 | Traditional Chinese medicine preparation for preventing abortion of sow |
| CN106420964A (en) * | 2016-10-10 | 2017-02-22 | 扬中牧乐药业有限公司 | An Tai Ling oral liquid and preparation method thereof |
| CN108498610A (en) * | 2018-06-22 | 2018-09-07 | 陈影 | One kind prevents miscarriage, tocolysis Chinese medicine compound prescription and preparation method thereof |
| CN108902036A (en) * | 2018-07-25 | 2018-11-30 | 西藏自治区农牧科学院畜牧兽医研究所 | A kind of cultural method improving hiding pig farrowing rate |
| CN110478457A (en) * | 2019-08-22 | 2019-11-22 | 福州市中医院 | A kind of Chinese medicine miscarriage prevention health sachet |
-
2002
- 2002-04-02 CN CNB02111241XA patent/CN1171612C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1375322A (en) | 2002-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2004204574A1 (en) | Herbal compositions for prostate conditions | |
| CN1857690A (en) | Gynecopathy treating preparation and its preparing process | |
| CN1765392A (en) | Chinese compound formulation for nourishing blood, regulating menstruation, stopping bleeding to prevent abortion and preparation method thereof | |
| CN1171612C (en) | Mixed Chinese medicine prepn. for treating natural miscrarriage | |
| CN1233389C (en) | Chinese materia medica preparation made from gen-seng and astragalus root for strengthening the body resistance and anti cancer as well as producing method | |
| CN1259955C (en) | Chinese medicine preparation for treating climacteric metancholia of women | |
| CN1843446A (en) | Combined matched pharmaceutical for treating irregular menses | |
| CN101337019A (en) | Oral traditional Chinese medicine formulation for treating herpes zoster post herpetic neuralgia and preparation method thereof | |
| CN1965951A (en) | Blood tonic capsule having ass hide and method for preparing same | |
| CN1899448A (en) | Chinese medicine compound preparation for treating amblyopia and its preparing method | |
| CN110742980B (en) | Traditional Chinese medicine composition for treating irritable bowel syndrome and preparation method and application thereof | |
| CN100336540C (en) | Medicine composition for treating hyperlipemia and its prepn | |
| CN1517115A (en) | Chinese patent drug-Fuyan capsdule for treating chronic pelvic inflammation | |
| CN101693086B (en) | Traditional Chinese medicine compound preparation for preventing recurrent aphthae, preparation method and application thereof | |
| CN105362382A (en) | Traditional Chinese medicine composition for treating recurrent oral ulcer and application thereof | |
| CN111558016A (en) | Traditional Chinese medicine composition with effects of protecting teeth and strengthening bones and traditional Chinese medicine preparation | |
| CN1211426A (en) | Medicine for treating diabetes | |
| CN1843460A (en) | Medicine for reducing blood sugar, blood pressure and improving microcirculation and immunity, and its preparation method | |
| CN1814125A (en) | Rehmannia-root preparation | |
| CN1274338C (en) | Traditional Chinese medicine for treating aplastic anemia and its preparation process | |
| CN1308031C (en) | Chinese medicine composition for treating biliary tract disease, preparing method and use | |
| CN1053381C (en) | Drug for promoting puerperal physiological recovery and curing postpartum disease | |
| CN1131065C (en) | Method for producing Naoxin'an Chinese medicinal capsule used for trating hemiparalysis, obstruction of qi in the chest and cardialgia | |
| CN101143191A (en) | Medicine capable of relieving side effects of mastocarinoma radiotherapy and chemotherapy | |
| CN1060939C (en) | Traditional Chinese medicine preparation for curing microcircalatory disturbance and its preparing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: SHIJIAZHUANG YILING MEDICINE CO., LTD. Free format text: FORMER OWNER: GYNAECOLOGY +. OBSTETRICS HOSPITAL ATTACHED TO FUDAN UNIV. Effective date: 20080801 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20080801 Address after: No. 238 Tianshan Street, hi tech Development Zone, Hebei, Shijiazhuang Patentee after: Shijiazhuang Yiling Pharmaceutical Co., Ltd. Address before: Shanghai City, fangxie Road No. 419 Patentee before: Gynaecology &. Obstetrics Hospital Attached to Fudan Univ. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20041020 |
|
| CX01 | Expiry of patent term |