CN115844937B - Preparation method and application of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects - Google Patents
Preparation method and application of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects Download PDFInfo
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Abstract
The invention relates to the technical field of functional foods, and discloses a preparation method and application of a common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain. The herba sonchifolii extract is pretreated through the processes of drying in the shade, crushing, sieving and the like, is optimized in terms of three factors of feed-liquid ratio, pH value and extraction time, and is subjected to membrane filtration, concentration, spray drying and the like to prepare the natural plant extract for dispelling effects of alcohol and protecting brain, so that the blank of the anti-alcohol medicine with the effects of dispelling effects of alcohol and protecting brain in the market is overcome.
Description
Technical Field
The invention relates to the technical field of functional foods, in particular to a preparation method and application of a common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain.
Background
At present, in the diet culture of China, wine is necessary for feast. The medicine proves that a small amount of drinking wine can accelerate blood circulation and eliminate fatigue, and some medicinal liquor also has the effect of treating diseases. Alcohol entering from the mouth enters the intestinal tract from the palace and the esophagus, the amount of alcohol absorbed in the stomach and the intestinal tract is respectively 25% and 75%, and the absorption speed and the absorption ratio are slightly different from those of food in the stomach. The absorbed alcohol enters the blood as it is, passes through the portal artery, reaches the heart through the liver, and then enters the large circulation system to permeate into various body fluid parts of the whole body, and the amount of the absorbed alcohol is proportional to the water content of various body fluids.
Alcohol is oxidized and excreted slowly in the human body, so that it is absorbed and accumulated in blood and various tissues for a long time, and the alcohol concentration in brain tissue is 10 times that of blood. Excess alcohol down regulates serotonin, resulting in inhibition and poisoning of the central nerve, respiratory center. Therefore, research and development of anti-alcohol products for alleviating hangover and protecting brain are of great significance.
The herba Sonchi Oleracei is annual or biennial herb of Sonchus genus of Compositae. It is often eaten as wild vegetable in folk. The Chinese plant Zhi and the Chinese herbal Assemblem record that whole herb of herba Sonchi Oleracei is used as medicine, and has the effects of clearing away heat and toxic materials, cooling blood and stopping bleeding. Can be used for treating enteritis, dysentery, acute icteric infectious hepatitis, appendicitis, mastitis, stomatitis, pharyngitis, tonsillitis, hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia; it is indicated for carbuncle, sore, swelling and toxicity, otitis media. Modern pharmacological researches have shown that common sow thistle has the functions of resisting oxidation, inflammation, tumor and ulcer.
At present, the anti-hangover drugs on the market have been developed successfully and have obvious curative effects, but no anti-hangover and brain-protecting products exist. Therefore, how to provide a natural plant extract which takes herba Sonchi arvensis as raw material and can be used for dispelling the effects of alcohol and protecting brain is a problem which needs to be solved by the person skilled in the art.
Disclosure of Invention
Therefore, the natural plant extract with the functions of dispelling the effects of alcohol and protecting the brain is provided in the research of the extraction process of the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain by optimizing three factors of the feed-liquid ratio, the pH value and the extraction time, and then performing membrane filtration, concentration and spray drying.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying aerial parts of herba Sonchi Oleracei in shade, pulverizing, and sieving to obtain herba Sonchi Oleracei powder;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:1-1:20 (g.mL-1) and pH value of 5-7, adding 10-15% (w/v) choline chloride, performing ultrasonic extraction, and centrifuging to obtain herba Sonchi arvensis extract;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) to obtain a filtrate with the molecular weight smaller than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution;
(5) And (3) spray-drying the concentrated solution obtained in the step (4) to obtain the herba sonchifolii extract.
The beneficial effect of above-mentioned scheme is: the herba Sonchi Oleracei extract with the functions of dispelling effects of alcohol and protecting brain is extracted by the scheme, so that the market blank is made up.
The temperature of the drying in the shade in the step (1) is 20-30 ℃ and the humidity is 10-20%.
And (3) crushing and sieving in the step (1) to obtain a 60-100 mesh sieve.
The beneficial effects of the above-mentioned further scheme are: the above mentioned drying and pulverizing conditions are selected, which is beneficial to the natural transformation of drug property in the treatment process, and can prevent the volatilization of volatile effective components, and improve the extraction efficiency and effect of herba Sonchi Oleracei extract with alcohol effect and brain protecting function.
The power of ultrasonic extraction in the step (2) is 450-550w, the temperature is 40-50 ℃ and the time is 20-40min.
The rotational speed of the centrifugation in the step (2) is 4000-5000rpm/min, and the time for centrifugation is 10-15min.
The beneficial effects of the above-mentioned further scheme are: the adoption of the condition parameters can effectively ensure the activity of the extract, and further improve the extraction efficiency, purity and effect of the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain.
The pressure of the membrane filtration in step (3) is 0.1-1.0mpa.
The vacuum degree of the reduced pressure concentration in the step (4) is-0.1 mpa to-0.08 mpa, and the temperature is 50-70 ℃.
The spray drying inlet temperature in the step (5) is 120-125 ℃, the feeding rate is 20-30mL/min, and the extraction amount is 60-80%.
On the other hand, the invention also provides an application of the herba sonchifoliae extract prepared by the method in the field of anti-alcohol brain-protecting health care.
Compared with the prior art, the preparation method of the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain has the following beneficial effects:
(1) Provides the herba Sonchi Oleracei extract with the functions of dispelling effects of alcohol and protecting brain and the preparation method thereof, and fills up the market blank with the dual functions of dispelling effects of alcohol and protecting brain;
(2) The invention obtains the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain by regulating and controlling the content of 5-hydroxytryptamine;
(3) The functional substances of the common sowthistle herb for dispelling the effects of alcohol and protecting the brain are defined, and the functional substances are compounds with the molecular weight of less than 500; (4) By limiting the condition parameters in the preparation method, the content of the active ingredients in the extract is improved, the loss of the active ingredients in the preparation process is reduced, and the production cost is reduced.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Experimental example
Method for detecting anti-alcohol and brain-protecting effects of common sow thistle extract
(1) 180 mice with similar physiological states are equally divided into 18 groups, and 10 mice in each group are respectively selected, wherein the group of the common sow thistle extract is 15 groups, the group of the normal sow thistle extract is 1 group and the group of the model sow thistle extract is 1 group. Mice were fasted for 12 hours prior to the test, and normal and model groups were given 10mL/kg of physiological saline, and the group of Sonchus arvensis extract was intragastrically at 0.3g/kg body weight. At 30min, normal group is given 10mL/kg physiological saline, the model group and herba Sonchi Oleracei extract group are infused with the same Chinese liquor at 10mL/kg body weight, mice are taken after 60min after the Chinese liquor is infused, and eyeballs are taken for blood collection.
(2) The gas phase conditions for detecting the ethanol content in the serum sample are chromatographic columns: DB-ALC2 (30 m×0.32mm×1.2 μm) quartz capillary column; column temperature: constant temperature 50 ℃; carrier gas: the purity of the nitrogen is more than or equal to 99.999 percent, and the flow rate is 3ml/min; sample inlet temperature: 200 ℃; detector temperature: 250 ℃; headspace sample feeding condition sample, sample bottle 70 ℃, quantitative loop 105 ℃, transmission line 110 ℃, gas phase cycle time: 5min; sample bottle heating equilibration time: 10.0min; sample bottle pressurization time: 0.10min; quantitative ring fill time: 0.10min; quantitative loop equilibration time: 0.05min; sample introduction time: 1.00min.
(3) High performance liquid chromatography is used for detecting the content of 5-hydroxytryptamine (5-HT) in blood, and chromatographic conditions are chromatographic columns: ODS-BP column (250 mm. Times.4.6 mm. Times.5 μm), mobile phase: 50mmol/LKH 2 PO 3 : methanol=95: 5 (pH is adjusted to 4.0 by phosphoric acid), the detection wavelength is 275nm, the flow rate is 1.0mL/min, the column temperature is 40 ℃, and the sample injection amount is 20 mu L.
Example 1
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 60 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 20 ℃ and the humidity is 10%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:10 (g.mL-1) with pH value of 5, adding 10% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 450w at 40deg.C for 20min, and centrifuging at 4000rpm/min for 10min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.1mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.1 mpa, and the temperature is 50 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 20mL/min, and the extraction amount is 60%, so as to obtain the common sow thistle extract.
Example 2
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 60 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 20 ℃ and the humidity is 10%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:10 (g.mL-1) with pH value of 6, adding 10% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 450w at 40deg.C for 20min, and centrifuging at 4000rpm/min for 10min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.1mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.1 mpa, and the temperature is 50 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 20mL/min, and the extraction amount is 60%, so as to obtain the common sow thistle extract.
Example 3
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 60 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 20 ℃ and the humidity is 10%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:10 (g.mL-1) with pH value of 7, adding 10% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 450w at 40deg.C for 20min, and centrifuging at 4000rpm/min for 10min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.1mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.1 mpa, and the temperature is 50 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 20mL/min, and the extraction amount is 60%, so as to obtain the common sow thistle extract.
Detection result
The difference detection of the anti-alcohol and brain-protecting effects of the common sow thistle extract prepared by extracting solvents with different pH values is shown in the following table:
example 4
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 30 ℃ and the humidity is 20%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:10 (g.mL-1) with pH value of 7, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 550w, ultrasonic extraction temperature of 50deg.C, ultrasonic extraction time of 40min, and centrifuging at 5000rpm/min for 15min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.08 mpa, and the temperature is 70 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding rate is 30mL/min, and the extraction rate is 80%, so as to obtain the common sow thistle extract.
Example 5
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 30 ℃ and the humidity is 20%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:15 (g.mL-1) with pH value of 7, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 550w, ultrasonic extraction temperature of 50deg.C, ultrasonic extraction time of 40min, and centrifuging at 5000rpm/min for 15min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.08 mpa, and the temperature is 70 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding rate is 30mL/min, and the extraction rate is 80%, so as to obtain the common sow thistle extract.
Example 6
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 30 ℃ and the humidity is 20%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 7, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 550w, ultrasonic extraction temperature of 50deg.C, ultrasonic extraction time of 40min, and centrifuging at 5000rpm/min for 15min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.08 mpa, and the temperature is 70 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding rate is 30mL/min, and the extraction rate is 80%, so as to obtain the common sow thistle extract.
Detection result
The difference detection of the anti-alcohol and brain-protecting effects of the common sow thistle extracts prepared by different feed liquid ratios is shown in the following table:
example 7
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of drying in the shade is 25 ℃ and the humidity is 15%
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 6, adding 13.5% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 500w at 45deg.C for 20min, and centrifuging at 4500rpm/min for 13.5min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.09 mpa, and the temperature is 60 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 123 ℃, the feeding rate is 25mL/min, and the extraction rate is 70%, so as to obtain the common sow thistle extract.
Example 8
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of drying in the shade is 25 ℃ and the humidity is 15%
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 6, adding 13.5% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 500w at 45deg.C for 30min, and centrifuging at 4500rpm/min for 13.5min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.09 mpa, and the temperature is 60 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 123 ℃, the feeding rate is 25mL/min, and the extraction rate is 70%, so as to obtain the common sow thistle extract.
Example 9
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of drying in the shade is 25 ℃ and the humidity is 15%
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 6, adding 13.5% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 500w at 45deg.C for 40min, and centrifuging at 4500rpm/min for 13.5min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.09 mpa, and the temperature is 60 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 123 ℃, the feeding rate is 25mL/min, and the extraction rate is 70%, so as to obtain the common sow thistle extract.
Detection result
The difference detection of the anti-alcohol and brain-protecting effects of the common sow thistle extract prepared at different extraction times is shown in the following table:
example 10
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of drying in the shade is 25 ℃ and the humidity is 15%
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) and pH value of 6, adding 5% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 500w at 45deg.C for 20min, and centrifuging at 4500rpm/min for 13.5min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.09 mpa, and the temperature is 60 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 25mL/min, and the extraction amount is 70%, so as to obtain the common sow thistle extract.
Example 11
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of drying in the shade is 25 ℃ and the humidity is 15%
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 6, adding 10% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 500w at 45deg.C for 20min, and centrifuging at 4500rpm/min for 13.5min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.09 mpa, and the temperature is 60 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 25mL/min, and the extraction amount is 70%, so as to obtain the common sow thistle extract.
Example 12
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of drying in the shade is 25 ℃ and the humidity is 15%
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) and pH value of 6, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 500w at 45deg.C for 20min, and centrifuging at 4500rpm/min for 13.5min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain a filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.09 mpa, and the temperature is 60 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 25mL/min, and the extraction amount is 70%, so as to obtain the common sow thistle extract.
Detection result
The difference detection of the anti-alcohol and brain-protecting effects of the common sow thistle extract prepared by different choline chloride addition amounts is shown in the following table:
example 13
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 30 ℃ and the humidity is 20%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 7, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 550w, ultrasonic extraction temperature of 50deg.C, ultrasonic extraction time of 40min, and centrifuging at 5000rpm/min for 15min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain a filtrate with the molecular weight of more than 800;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.08 mpa, and the temperature is 70 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding rate is 30mL/min, and the extraction rate is 80%, so as to obtain the common sow thistle extract.
Example 14
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 30 ℃ and the humidity is 20%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 7, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 550w, ultrasonic extraction temperature of 50deg.C, ultrasonic extraction time of 40min, and centrifuging at 5000rpm/min for 15min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the herba Sonchi arvensis extract obtained in the step (2) under the pressure of 1.0mpa to obtain filtrate with the molecular weight of 500-800;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.08 mpa, and the temperature is 70 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding rate is 30mL/min, and the extraction rate is 80%, so as to obtain the common sow thistle extract.
Example 15
A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the temperature of the drying in the shade is 30 ℃ and the humidity is 20%;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL-1) with pH value of 7, adding 15% (w/v) choline chloride, performing ultrasonic extraction with ultrasonic extraction power of 550w, ultrasonic extraction temperature of 50deg.C, ultrasonic extraction time of 40min, and centrifuging at 5000rpm/min for 15min to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the herba Sonchi arvensis extract obtained in the step (2) under the pressure of 1.0mpa to obtain filtrate with molecular weight less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution, wherein the vacuum degree is-0.08 mpa, and the temperature is 70 ℃;
(5) And (3) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding rate is 30mL/min, and the extraction rate is 80%, so as to obtain the common sow thistle extract.
Detection result
The difference detection of the anti-alcohol and brain-protecting effects of the common sow thistle extracts with different molecular weight ranges is shown in the following table:
in summary, the above examples were compared in five dimensions of feed-liquid ratio, pH value of extraction solution, extraction time, choline chloride addition amount, and molecular weight obtained by membrane filtration, and it was found that the extract obtained by concentration and spray drying had good anti-hangover and brain-protecting effects by extracting under conditions of feed-liquid ratio ranging from 1:15 to 1:20 (g.mL-1), pH value ranging from 5 to 7, extraction time ranging from 30 to 40min, choline chloride addition amount ranging from 10 to 15% (w/v)
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A preparation method of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects is characterized by comprising the following steps:
(1) Drying aerial parts of herba Sonchi Oleracei in shade, pulverizing, and sieving to obtain herba Sonchi Oleracei powder;
(2) Adding herba Sonchi Oleracei powder into 50% v/v ethanol solution, wherein the ratio of feed to liquid is 1:15-1:20 g/mL -1 Adding 10-15% w/v choline chloride at pH of 5-7, performing ultrasonic extraction with power of 450-550w at 40-50deg.C for 20-40min, and centrifuging to obtain herba Sonchi Oleracei extractive solution;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) to obtain a filtrate with the molecular weight smaller than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution;
(5) And (3) spray-drying the concentrated solution obtained in the step (4) to obtain the herba sonchifolii extract.
2. The method according to claim 1, wherein the temperature of the drying in the shade in step (1) is 20 to 30 ℃ and the humidity is 10 to 20%.
3. The method of claim 1, wherein the comminuting is carried out in step (1) by sieving through a 60-100 mesh sieve.
4. The method according to claim 1, wherein the rotational speed of the centrifugation in the step (2) is 4000 to 5000rpm/min and the centrifugation time is 10 to 15min.
5. The method of claim 1, wherein the membrane filtration in step (3) is performed at a pressure of 0.1 to 1.0mpa.
6. The method according to claim 1, wherein the vacuum degree of the reduced pressure concentration in the step (4) is-0.1 mpa to-0.08 mpa, and the temperature is 50 to 70 ℃.
7. The method according to claim 1, wherein the spray-drying inlet temperature in step (5) is 120-125 ℃, the feed rate is 20-30mL/min, and the extraction rate is 60-80%.
8. Use of herba Sonchi Oleracei extract prepared by the method of any one of claims 1-7 in preparation of health product for relieving hangover and protecting brain.
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| 超声波辅助提取苦苣总黄酮的工艺研究;高辉等;《吉林医药学院学报》;第33卷(第1期);第20-22页 * |
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