CN116474020A - Sonchus arvensis jujube extract for improving sleep and application thereof - Google Patents
Sonchus arvensis jujube extract for improving sleep and application thereof Download PDFInfo
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- CN116474020A CN116474020A CN202310535792.1A CN202310535792A CN116474020A CN 116474020 A CN116474020 A CN 116474020A CN 202310535792 A CN202310535792 A CN 202310535792A CN 116474020 A CN116474020 A CN 116474020A
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- 230000007958 sleep Effects 0.000 title claims abstract description 36
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- 235000006731 Sonchus arvensis Nutrition 0.000 title description 13
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- 238000001035 drying Methods 0.000 claims abstract description 7
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- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/20—Hypnotics; Sedatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The invention discloses a chicory jujube extract for improving sleep, which comprises a jujube extract and a common sow thistle extract, wherein the mass ratio of the jujube extract to the common sow thistle extract is 1-3:3-1; the preparation method of the common sow thistle extract comprises the following steps: (1) Adding herba Sonchi Oleracei into a mixed solution of choline chloride and ethanol, and performing ultrasonic extraction to obtain an extract; (2) Filtering the extractive solution, concentrating, and drying to obtain herba Sonchi Oleracei extract. According to the invention, the medicinal and edible common sow thistle and the Chinese dates which can be used for health-care food are selected for compatibility, so that the synergistic sleep improving function can be achieved, and a theoretical basis is provided for developing the extract of the common sow thistle and the Chinese dates into health-care food for improving the sleep function.
Description
Technical Field
The invention relates to the technical field of natural medicines, in particular to a chicory jujube extract for improving sleep and application thereof.
Background
Insomnia is a common clinical disease, and is mainly characterized by difficult sleep, difficult sleep maintenance or poor sleep quality, incapacitation of fatigue, physical strength recovery and the like, and in recent years, the incidence rate of insomnia of Chinese adults is up to 38.2 percent, especially under the environment of infectious diseases such as influenza and the like, 40 percent of people have the problem of poor sleep quality. Insomnia not only affects daily work and study of people, but also brings serious damage to physical and mental health, and increases occurrence probability of chronic diseases such as mental, cardiovascular and cerebrovascular diseases, diabetes and the like. Sufficient sleeping time can promote normal development and growth of organisms, relieve fatigue, ensure high-efficiency operation of brains and is necessary for human survival. Therefore, the problem of insomnia is particularly urgent.
At present, the drug for treating the sleep disorder is the first choice, and main drugs comprise barbiturates, benzodiazepines, non-benzodiazepines, melatonin and some antidepressants, but the drugs can produce dependence, addiction, withdrawal symptoms, rebound phenomenon and other toxic and side effects after drug withdrawal after long-term administration, so the research and development of the drug for safely and efficiently improving the sleep is not easy. In recent years, a great deal of reports prove that medicinal and edible plants such as ganoderma lucidum, moringa oleifera, maca, kiwi fruit, ginseng, passion fruit and the like are rich in various nutrition and functional active substances, have good sleep improving effect, and have small side effects and strong activity compared with chemical medicaments, so that the medicinal and edible plants are valued by students at home and abroad, the research center of gravity is gradually turned to search for functional factors for improving sleep from natural products, and the natural extract effect in the prior art cannot meet the demands of people.
Therefore, providing an extract with good effect for improving sleep and a preparation method thereof are technical problems to be solved in the field.
Disclosure of Invention
The invention aims to provide a chicory jujube extract for improving sleep and application thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a herba Sonchi Oleracei extract for improving sleep comprises fructus Jujubae extract and herba Sonchi Oleracei extract at a mass ratio of 1-3:3-1.
Preferably, the mass ratio of the jujube extract to the common sow thistle extract is 1:1.
Preferably, the preparation method of the common sow thistle extract comprises the following steps:
(1) Adding herba Sonchi Oleracei into a mixed solution of choline chloride and ethanol, and performing ultrasonic extraction to obtain an extract;
(2) Filtering the extractive solution, concentrating, and drying to obtain herba Sonchi Oleracei extract.
The preparation method of the herba Sonchi arvensis extract is based on Chinese patent No. CN202211641613.4, and the method improves the content of effective components in the extract, reduces the loss of the effective components in the preparation process, and reduces the production cost.
Preferably, the herba Sonchi Oleracei in step (1) is herba Sonchi Oleracei powder obtained by pulverizing aerial parts of herba Sonchi Oleracei.
Preferably, the preparation method of the common sow thistle powder comprises the following steps: drying aerial parts of herba Sonchi Oleracei in shade at 20-30deg.C and humidity of 10-20%, pulverizing, and sieving with 60-100 mesh sieve to obtain herba Sonchi Oleracei powder.
Preferably, in the step (1), the concentration of the ethanol is 50%, and the mass-volume ratio of the common sow thistle to the 50% ethanol solution is 1g to 1-20mL;
the concentration of the choline chloride is 10-15%.
Preferably, the filtration in step (2) results in a filtrate having a molecular weight of less than 500;
the concentration is carried out until the density is 1.05-1.14.
Preferably, the preparation method of the jujube extract comprises the following steps: adding fructus Jujubae into water, extracting, concentrating the extractive solution, and drying to obtain fructus Jujubae extract.
Preferably, the feed liquid ratio of the jujube to the water is 1g:8-10mL;
the extraction time is 1-2h, and the temperature is 60 ℃.
Preferably, the concentration is to a density of 1.05-1.1.
The application of the chicory jujube extract in preparing medicaments, health-care products or foods for improving sleep is provided.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a chicory jujube extract for improving sleep and application thereof.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described, and the drawings in the description are only embodiments of the present invention.
FIG. 1 is a line graph showing the weight change of mice in each group according to the application example of the present invention.
Detailed Description
The following describes embodiments of the invention, examples of which are illustrated in the accompanying drawings, and the embodiments described with reference to the drawings are illustrative and intended to be in the way of explanation of the invention and not to be construed as limiting the invention.
Example 1
A preparation method of a chicory jujube extract for improving sleep specifically comprises the following steps:
1. the preparation of the common sow thistle extract comprises the following steps:
(1) Drying herba Sonchi Oleracei aerial parts in shade (at 20-30deg.C and 10-20% humidity), pulverizing, and sieving (100 mesh sieve) to obtain herba Sonchi Oleracei powder;
(2) Adding herba Sonchi Oleracei powder into 50% (v/v) ethanol solution, wherein the ratio of feed to liquid is 1:20 (g.mL) -1 ) Adding 10% (w/v) choline chloride into the solution with pH value of 5-7, performing ultrasonic extraction (ultrasonic temperature is 40 ℃ and ultrasonic power is 450 w), and centrifuging to obtain herba Sonchi Oleracei extract;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) to obtain a filtrate with the molecular weight smaller than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain concentrated solution with the density of 1.05;
(5) And (3) spray-drying the concentrated solution obtained in the step (4) to obtain the herba sonchifolii extract.
2. The preparation of the jujube extract comprises the following steps:
(1) According to the feed liquid (jujube: water) ratio of 1:10 (g.mL) -1 ) Extracting under reduced pressure at 60deg.C for 1 hr, and filtering to obtain fructus Jujubae extractive solution;
(2) Concentrating the jujube extract obtained in the step (1) under reduced pressure to obtain a concentrated solution with a concentration density of 1.05;
(3) Spray drying the concentrated solution obtained in the step (2) to obtain a jujube extract;
3. preparation of Sonchus arvensis jujube extract
Compounding the prepared herba Sonchi Oleracei extract and fructus Jujubae extract at a ratio of 1:1, 1:3 and 3:1 to obtain herba Sonchi Oleracei fructus extract for use.
Application example
The animal experiments were as follows:
1. grouping and administration of animals
ICR strain mice are used as male and female, the weight of the mice is 18-20g, and 60 mice are randomly divided into 6 groups according to the mass of the mice, and 10 mice in each group; the compositions are a blank group, a Chinese date extract group, a common sow thistle extract group, a Chinese date extract+common sow thistle extract (1:1) group, a Chinese date extract+common sow thistle extract (1:3) group and a Chinese date extract+common sow thistle extract (3:1) group respectively; preparing samples with different concentrations by distilled water every day, and performing gastric lavage administration by 20mL/kg, and performing gastric lavage for 30d continuously by administering distilled water with the same volume for 1 time every day in a blank control group;
2. observation index and measurement method
(1) Effects on direct sleep and voluntary activity in mice: 30min after the last administration, placing the mice into an autonomous activity instrument of the mice to adapt for 5min, and recording the autonomous activity times of the mice within 5 min; observing the number of animals falling asleep and the sleeping time within 1h of administration, wherein sleep takes the disappearance of the regular reflection as an index;
(2) Prolonged pentobarbital sodium sleep time experiment: after the mice are last dosed for 30min, 47.5mg/kg of pentobarbital sodium is injected into the abdominal cavity of each group of animals, the injection volume is 10mL/kg, the loss of the specular reflection is taken as an index, the time for the animals to fall asleep and the time for the animals to wake up are observed and recorded, and the sleeping time is calculated;
(3) Pentobarbital sodium subthreshold dose hypnotic experiment: after the last administration of mice for 30min, injecting 27.5mg/kg of pentobarbital sodium into the abdominal cavity of each group of animals, wherein the injection volume is 10mL/kg, placing the animals in a quiet environment at 24-25 ℃, observing the animals to fall asleep after the eversion and the disappearance of the regular reflection for more than 1min, and recording the number of the animals falling asleep within 30min of each group;
(4) Barbital sodium sleep latency experiment: after the mice are dosed for 20:min last time, each group of animals is injected with 250mg/kg of barbital sodium in the abdominal cavity, the injection volume is 20mL/kg, after the injection of the barbital sodium is observed, the eversion and the disappearance of the specular reflection are used as the sleep-on indexes for more than 1min, the sleep-on time of the animals is recorded, and the sleep latency is calculated;
(5) Determination of 5-HT content in mice brain: after the experimental mice are tested and observed, cervical vertebra is removed, the brains are separated on an ice plate after the brains are opened by ice bath, the blood is washed by ice normal saline, the filter paper is wiped dry, 9 times of ice normal saline is added after the brains are weighed, the slurry is homogenized at 4 ℃, the slurry is taken into a 2mL centrifuge tube for centrifugation (3000 rpm,15min and 4 ℃), the supernatant is taken, the OD value of 5-HT in a sample is measured according to the relevant operation steps of the kit, and the OD value is brought into a standard curve regression equation, and the content of 5-HT in the sample is calculated;
wherein, the synergy evaluates the compounding capability through experimental calculation of an interaction index gamma
Theoretical IC of compound 50add The value calculation formula is IC 50add ＝IC 50A /(K 1 +Q×K 2 )；
Wherein: q is the cost/effective ratio of the two extracts of A, B acting alone, i.e. q=ic 50A /IC 50B ；K 1 、K 2 Respectively the proportion of the jujube extract and the common sow thistle extract in the compound system, K 2 ＝1-K 1 ；
The actual IC of the complex can be calculated by the above experiment 50mix The value is obtained to obtain the IC concentration theoretically required after the combination of the jujube extract and the common sow thistle extract 50add Value, compare the two, if IC 50mix ＜IC 50add The effect of improving sleep after two samples are compounded can be proved, the strength of the synergistic effect between the compounds is expressed by gamma value, and the calculation formula is gamma=IC 50Amix /IC 50A +IC 50Bmix /IC 50B ；
Wherein: IC (integrated circuit) 50Amix 、IC 50Bmix IC of jujube extract and sonchus extract in the compound system respectively 50 A value; IC (integrated circuit) 50A 、IC 50B IC of two extracts of fructus Jujubae extract and herba Sonchi Oleracei extract 50 A value; if γ=1, the interactions are additive; if gamma is less than 1, the synergy exists between the two, and the lower the gamma value is, the stronger the synergy is; if γ > 1, the interaction is antagonistic.
SPSS 19.0 software is adopted for statistical analysis, the metering data result is expressed by (x+/-s), a plurality of groups of data are compared by single-factor analysis of variance, and the comparison between the two groups adopts t-test; x for counting data 2 The test (exact probability method) has statistical significance with p <0.05 as the difference.
3 results of experiments
(1) Effect of Sonchus arvensis jujube extract on mouse body weight
Fig. 1 is a line graph showing weight change of mice in each group, and as shown in fig. 1, the initial weights of the mice are not significantly different from the weights of the mice at 7 th, 14 th, 21 th and 30d (P > 0.05), and the daily activities of the mice are normal, so that adverse reactions do not occur, indicating that the weight growth of the mice is not significantly affected by the common sow herb extract, the Chinese date extract and the Chinese date extract, and no side effects are generated;
(2) Effect of Sonchus arvensis jujube extract on direct sleep and voluntary Activity in mice
After administration, no sleeping animals are seen in each dose group, indicating no effect on direct sleep, wherein the number of independent activities of mice in the group of the common sow thistle extract, the Chinese date extract and the common sow thistle extract is reduced, and compared with the blank control group, the effect of the common sow thistle extract and the Chinese date extract is obviously different (p is less than 0.05), and the result is shown in table 1;
TABLE 1 comparison of direct sleep and voluntary Activity in groups of mice (x.+ -. S)
(3) Effect of Sonchus arvensis jujube extract on prolonging sleep time of pentobarbital sodium-induced mice
As can be seen from the comparison of the sleep time of the pentobarbital sodium-induced mice prolonged by the mice in each group in table 2, the sleep time of the pentobarbital sodium-induced mice can be obviously prolonged by the group of the common sowthistle extract, the Chinese date extract and the group of the common sowthistle extract, and compared with the blank control group, the effect of the common sowthistle extract and the Chinese date extract is superior;
table 2 comparison of the mice groups for prolonged sleep time of pentobarbital sodium-induced mice (x.+ -. S, min)
| Grouping | Number of animals | Time to fall asleep | Sleep time |
| Control group | 10 | 5.49±0.31 | 25.1±5.6 |
| Sonchus arvensis extract group | 10 | 4.21±0.26 | 39.2±6.3** |
| Jujube extract group | 10 | 5.01±0.19 | 28.7±6.5 |
| Herba Sonchi Oleracei extract+fructus Jujubae extract 1:1 | 10 | 4.05±0.35 | 35.2±7.2** |
| Herba Sonchi Oleracei extract+fructus Jujubae extract 1:3 | 10 | 3.56±0.25 | 45.9±5.3** |
| Herba Sonchi Oleracei extract+fructus Jujubae extract 3:1 | 10 | 3.98±0.18 | 41.6±4.9** |
(4) Effect of Sonchus arvensis jujube extract on sub-threshold dose hypnotic effect of sodium pentobarbital
Table 3 shows that the comparison of the sleeping occurrence rate of the mice with the subthreshold dose of sodium pentobarbital in each group can increase the number of sleeping animals, compared with the blank control group, the effect of the herba Sonchi arvensis extract, the effect of the fructus ziziziphi extract is superior to that of the herba Sonchi arvensis extract and the effect of the fructus ziphi extract are obvious (p < 0.05);
table 3 comparison of sleep incidence in sub-threshold dose mice of sodium pentobarbital for each group of mice
(5) Effect of Sonchus arvensis jujube extract on the sodium barbiturate sleep latency in mice
Table 4 shows that the group of common sow thistle extract, the group of Chinese date extract and the group of the Chinese date extract can obviously shorten the sleeping latency of the mice with the barbituric sodium compared with the blank control group, and the effect of the Chinese date extract is better than that of the group of the common sow thistle extract and the group of the Chinese date extract.
Table 4 comparison of barbital sodium sleep latency in mice of each group (x.+ -. S, min)
| Grouping | Number of animals | Sleep latency |
| Control group | 10 | 24.6±2.3 |
| Sonchus arvensis extract group | 10 | 15.2±4.7** |
| Jujube extract group | 10 | 19.3±1.2 |
| Herba Sonchi Oleracei extract+fructus Jujubae extract 1:1 | 10 | 13.1±3.8** |
| Herba Sonchi Oleracei extract+fructus Jujubae extract 1:3 | 10 | 9.6±4.2** |
| Herba Sonchi Oleracei extract+fructus Jujubae extract 3:1 | 10 | 11.5±3.5** |
(6) Effect of Sonchus arvensis jujube extract on mouse brain 5-HT content
Table 5 shows that the comparison of the 5-HT content of the brain tissue of each group of mice, compared with the blank control group, the group of the common sow thistle extract, the Chinese date extract and the group of the chicory date extract can obviously increase the 5-HT content in the brain of the mice (the p is less than 0.05), in particular to the group of the chicory date extract;
table 5 comparison of 5-HT content in brain tissue of mice of each group (x.+ -. S)
(7) Synergistic sleep improvement of chicory jujube extract
Table 6 shows the synergistic index results of the extracts of Sonchus arvensis, and the theoretical IC50 mix Value ratio test IC50 add The value of the product is small and the product is stable,the gamma value of each combination is smaller than 1, namely, the effect of improving the 5-HT content in the brain after the jujube extract and the common sow thistle extract are recombined according to different proportions is proved to be stronger than that of the independent effect, and the synergistic effect is achieved;
TABLE 6 synergistic index results of Sonchus arvensis jujube extract
Therefore, it is known from the above that the group of the common sow thistle extract, the Chinese date extract and the chicory date extract all have the function of improving sleep, and particularly the chicory date extract can synergistically improve sleep by increasing the 5-HT content in the brain.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The chicory jujube extract for improving sleep is characterized by comprising a jujube extract and a herba Sonchi arvensis extract, wherein the mass ratio of the jujube extract to the herba Sonchi arvensis extract is 1-3:3-1.
2. The sleep improving chicory date extract of claim 1, wherein the mass ratio of the jujube extract to the chicory extract is 1:1.
3. The method for preparing the chicory jujube extract for improving sleep according to claim 1, wherein the method for preparing the chicory extract comprises the following steps:
(1) Adding herba Sonchi Oleracei into a mixed solution of choline chloride and ethanol, and performing ultrasonic extraction to obtain an extract;
(2) Filtering the extractive solution, concentrating, and drying to obtain herba Sonchi Oleracei extract.
4. The method of claim 3, wherein the herba Sonchi arvensis extract obtained by pulverizing the aerial parts of herba Sonchi arvensis in step (1) is herba Sonchi arvensis powder.
5. A chicory jujube extract for improving sleep according to claim 3, characterized in that in step (1) the concentration of ethanol is 50%, the mass-to-volume ratio of the herba Sonchi arvensis and the 50% ethanol solution is 1g:1-20mL;
the concentration of the choline chloride is 10-15%.
6. A chicory jujube extract for improving sleep according to claim 3, characterized in that said filtration in step (2) results in a filtrate with a molecular weight of less than 500;
the concentration is carried out until the density is 1.05-1.14.
7. The method for preparing the extract of the chicory date for improving sleep according to claim 1, wherein the preparation method of the extract of the date comprises the following steps: adding fructus Jujubae into water, extracting, concentrating the extractive solution, and drying to obtain fructus Jujubae extract.
8. A chicory date extract for improving sleep according to claim 7, wherein the ratio of feed to water is 1g:8-10mL;
the extraction time is 1-2h, and the temperature is 60 ℃.
9. The extract of chicory date for improving sleep of claim 7, wherein said concentration is to a density of 1.05-1.1.
10. Use of the extract of chicory date according to any of claims 1 to 9, for the preparation of a medicament, a health product or a food for improving sleep.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331978A (en) * | 2000-06-22 | 2002-01-23 | 中国科学院植物研究所 | Application of sowthistle in preparing tobacco toxin resisting medicine |
| CN115844937A (en) * | 2022-12-20 | 2023-03-28 | 北京暄芷达生物科技有限公司 | Preparation method and application of sonchus oleraceus extract with functions of dispelling effects of alcohol and protecting brain |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331978A (en) * | 2000-06-22 | 2002-01-23 | 中国科学院植物研究所 | Application of sowthistle in preparing tobacco toxin resisting medicine |
| CN115844937A (en) * | 2022-12-20 | 2023-03-28 | 北京暄芷达生物科技有限公司 | Preparation method and application of sonchus oleraceus extract with functions of dispelling effects of alcohol and protecting brain |
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