CN115844937A - Preparation method and application of sonchus oleraceus extract with functions of dispelling effects of alcohol and protecting brain - Google Patents
Preparation method and application of sonchus oleraceus extract with functions of dispelling effects of alcohol and protecting brain Download PDFInfo
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Abstract
The invention relates to the technical field of functional foods, and discloses a preparation method and application of a common sow thistle extract with functions of dispelling effects of alcohol and protecting brain. The common sow thistle extract is pretreated by processes of drying in the shade, crushing, sieving and the like, then is optimized according to three factors of material-liquid ratio, pH value and extraction time, and is subjected to membrane filtration, concentration, spray drying and the like to prepare the natural plant extract for relieving alcoholism and protecting the brain, so that the blank of the dealcoholic medicine with the effects of relieving alcoholism and protecting the brain in the market is filled.
Description
Technical Field
The invention relates to the technical field of functional foods, in particular to a preparation method and application of a common sow thistle extract with functions of dispelling effects of alcohol and protecting brain.
Background
At present, wine must be provided for banquet in the dietary culture of China. The medicine proves that a small amount of drinking can accelerate blood circulation and eliminate fatigue, and some medicinal liquors also have the effect of treating diseases. The alcohol entering from the mouth enters the intestinal tract together with the esophagus, the amount of alcohol absorbed in the stomach and the intestinal tract is 25% and 75% respectively, and the speed and the proportion of the absorption are slightly different with the food in the stomach. The absorbed alcohol enters the blood without moving, passes through the portal artery, reaches the heart through the liver, enters the major circulation system, and permeates into each body fluid part of the whole body, and the amount of the absorbed alcohol is proportional to the water content in each body fluid.
Alcohol is oxidized and excreted slowly in the human body, so it is absorbed and accumulated in blood and various tissues for a long time, and the alcohol concentration in brain tissue is 10 times of that in blood. Excess alcohol down-regulates serotonin, resulting in inhibitory and toxic effects on the central nervous and respiratory centres. Therefore, the research and development of the anti-alcoholism product for relieving alcoholism and protecting the brain have important significance.
Herba Sonchi arvensis is of Sonchi arvensis of Compositae and annual or biennial herb. Is often eaten as a wild vegetable in folks. According to records in Chinese plant journal and national Chinese herbal compilation, the whole herb of sonchus oleraceus is used as a medicine for clearing away heat and toxic materials, cooling blood and stopping bleeding. Can be used for treating enteritis, dysentery, acute icterohepatitis, appendicitis, mastitis, stomatitis, pharyngitis, tonsillitis, hematemesis, epistaxis, hemoptysis, hematochezia, and metrorrhagia; it is used externally to treat carbuncle, sore, swelling and toxin and otitis media. Modern pharmacological research shows that the common sow thistle has the effects of oxidation resistance, inflammation resistance, tumor resistance, ulcer resistance and the like.
At present, the anti-alcoholism drugs on the market are successfully developed and have obvious curative effect, but no product with the effects of relieving alcoholism and protecting brain is available. Therefore, the problem that needs to be solved by the technical personnel in the field is how to provide a natural plant extract which takes common sow thistle as a raw material and can be used for relieving alcoholism and protecting brain.
Disclosure of Invention
In view of the above, the invention provides a natural plant extract with the function of relieving alcoholism and protecting brain by optimizing three factors of feed-liquid ratio, pH value and extraction time, membrane filtering, concentrating and spray drying in the process of researching the extraction process of the common sow thistle extract with the function of relieving alcoholism and protecting brain.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial parts of herba Sonchi Oleracei in shade, pulverizing, and sieving to obtain herba Sonchi Oleracei powder;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:1-1:20 (g.mL-1), pH 5-7, adding 10-15% (w/v) choline chloride, performing ultrasonic extraction, and centrifuging to obtain herba Sonchi arvensis extract;
(3) Membrane filtering the sonchus oleraceus extract obtained in the step (2) to obtain filtrate with molecular weight less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain a concentrated solution;
(5) And (4) spray-drying the concentrated solution obtained in the step (4) to obtain the common sow thistle extract.
The beneficial effect of above-mentioned scheme does: the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain is extracted by the scheme, and the market blank is filled.
In the step (1), the drying temperature in the shade is 20-30 ℃, and the humidity is 10-20%.
In the step (1), the crushing and sieving is to pass through a 60-100 mesh sieve.
The beneficial effects of the above further scheme are: the above drying and crushing conditions are adopted, which is beneficial to natural transformation of drug properties in the treatment process, and can also prevent volatilization of volatile effective components, and improve the extraction efficiency and effect of the sonchus oleraceus extract for understanding the brain-protecting function of wine.
The ultrasonic extraction power in the step (2) is 450-550w, the temperature is 40-50 ℃, and the time is 20-40min.
The rotating speed of the centrifugation in the step (2) is 4000-5000rpm/min, and the time of the centrifugation is 10-15min.
The beneficial effects of the above further scheme are: by adopting the condition parameters, the activity of the extract can be effectively guaranteed, and the extraction efficiency, purity and effect of the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain can be further improved.
The pressure of the membrane filtration in the step (3) is 0.1-1.0mpa.
The vacuum degree of the decompression concentration in the step (4) is-0.1 mpa to-0.08 mpa, and the temperature is 50-70 ℃.
In the step (5), the inlet temperature of the spray drying is 120-125 ℃, the feeding speed is 20-30mL/min, and the air extraction amount is 60-80%.
On the other hand, the invention also provides application of the common sow thistle extract prepared by the method in the fields of alcohol dispelling, brain protecting and health care.
According to the technical scheme, compared with the prior art, the preparation method of the sonchus oleraceus extract with the functions of dispelling the effects of alcohol and protecting the brain disclosed by the invention has the following beneficial effects:
(1) Provides a common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain and a preparation method thereof, and fills the market blank of having the double effects of dispelling the effects of alcohol and protecting the brain;
(2) The invention obtains the common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain by regulating and controlling the content of 5-hydroxytryptamine;
(3) The effective substance of sonchus oleraceus for relieving alcoholism and protecting brain is defined, and the effective substance is a compound with the molecular weight of less than 500; (4) By limiting the condition parameters in the preparation method, the content of the effective components in the extract is improved, the loss of the effective components in the preparation process is reduced, and the production cost is reduced.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Examples of the experiments
Method for detecting anti-alcoholism and brain-protecting effects of sonchus oleraceus extract
(1) Taking 180 mice with similar physiological state, averagely dividing into 18 groups of 10 mice each, wherein the group of the common sow thistle extract comprises 15 groups, a normal group 1 group and a model group 1 group. The mice were fasted for 12h before the test, the normal group and the model group were given 10mL/kg of physiological saline, and the sonchus oleraceus extract group was gavaged with 0.3g/kg of body weight. The normal group is administered with 10mL/kg normal saline 30min, the model group and herba Sonchi arvensis extract group are administered with the same Chinese liquor 10mL/kg body weight, and after administration of Chinese liquor, respectively taking out mouse 60min later, taking out eyeball and collecting blood for use.
(2) The gas phase condition for detecting the ethanol content in the serum sample is that a chromatographic column: DB-ALC2 (30 m × 0.32mm × 1.2 μm) quartz capillary column; column temperature: keeping the temperature at 50 ℃; carrier gas: nitrogen with purity more than or equal to 99.999 percent and flow rate of 3ml/min; sample inlet temperature: 200 ℃; detector temperature: 250 ℃; headspace sample injection conditions, sample bottle 70 ℃, quantitative loop 105 ℃, transmission line 110 ℃, gas phase circulation time: 5min; sample bottle heating equilibration time: 10.0min; sample bottle pressurization time: 0.10min; quantitative loop fill time: 0.10min; quantitative ring equilibration time: 0.05min; sample introduction time: 1.00min.
(3) Detecting the content of 5-hydroxytryptamine (5-HT) in blood by high performance liquid chromatography under the chromatographic column conditions: ODS-BP column (250 mm. Times.4.6 mm. Times.5 μm), mobile phase: 50mmol/LKH 2 PO 3 : methanol =95:5 (phosphoric acid adjusted to pH 4.0), a detection wavelength of 275nm, a flow rate of 1.0mL/min, a column temperature of 40 ℃ and a sample injection amount of 20. Mu.L.
Example 1
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 60 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 20 ℃, and the humidity is 10%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:10 (g.mL-1), the pH value is 5, then 10% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 450w, the ultrasonic extraction temperature is 40 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifuging for 10min at 4000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.1mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.1 mpa and temperature of 50 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 20mL/min, and the air extraction amount is 60%, so that the common sow thistle extract is obtained.
Example 2
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 60 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 20 ℃, and the humidity is 10%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:10 (g.mL-1), the pH value is 6, then 10% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 450w, the ultrasonic extraction temperature is 40 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifuging for 10min at 4000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.1mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.1 mpa and temperature of 50 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 20mL/min, and the air extraction amount is 60%, so that the common sow thistle extract is obtained.
Example 3
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 60 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 20 ℃, and the humidity is 10%;
(2) Adding sow thistle powder into 50% (v/v) ethanol solution, wherein the ratio of material to liquid is 1:10 (g.mL-1), the pH value is 7, then 10% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 450w, the ultrasonic extraction temperature is 40 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifuging for 10min at 4000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.1mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.1 mpa and temperature of 50 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 20mL/min, and the air extraction amount is 60%, so that the common sow thistle extract is obtained.
The result of the detection
The difference detection of the anti-alcoholism and brain-protecting effects of the sonchus oleraceus extract prepared by extracting solvents with different pH values is shown in the following table:
example 4
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 30 ℃, and the humidity is 20%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:10 (g.mL-1), the pH value is 7, 15% (w/v) choline chloride is added for ultrasonic extraction, the ultrasonic extraction power is 550w, the ultrasonic extraction temperature is 50 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extract is obtained by centrifugation for 15min at 5000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.08 mpa and temperature of 70 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding speed is 30mL/min, and the air extraction amount is 80%, so that the common sow thistle extract is obtained.
Example 5
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 30 ℃, and the humidity is 20%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:15 (g.mL-1), the pH value is 7, then 15% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 550w, the ultrasonic extraction temperature is 50 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extracting solution is obtained by centrifugation for 15min at 5000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.08 mpa and temperature of 70 deg.C;
(5) And (5) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding speed is 30mL/min, and the air suction amount is 80%, so that the common sow thistle extract is obtained.
Example 6
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 30 ℃, and the humidity is 20%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 7, then 15% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 550w, the ultrasonic extraction temperature is 50 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extracting solution is obtained by centrifugation for 15min at 5000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.08 mpa and temperature of 70 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding speed is 30mL/min, and the air extraction amount is 80%, so that the common sow thistle extract is obtained.
The result of the detection
The difference detection of the anti-alcoholism and brain-protecting effects of the sonchus oleraceus extract prepared by different feed-liquid ratios is shown in the following table:
example 7
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 25 deg.C, and the humidity is 15%
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 6, then 13.5% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 500w, the ultrasonic extraction temperature is 45 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifugation at 4500rpm/min for 13.5 min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.09 mpa and temperature of 60 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 123 ℃, the feeding speed is 25mL/min, and the air suction amount is 70%, so that the common sow thistle extract is obtained.
Example 8
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 25 deg.C, and the humidity is 15%
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 6, then 13.5% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 500w, the ultrasonic extraction temperature is 45 ℃, the ultrasonic extraction time is 30min, and the common sow thistle extract is obtained by centrifugation at 4500rpm/min for 13.5 min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.09 mpa and temperature of 60 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 123 ℃, the feeding speed is 25mL/min, and the air suction amount is 70%, so that the common sow thistle extract is obtained.
Example 9
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 25 deg.C, and the humidity is 15%
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 6, then 13.5% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 500w, the ultrasonic extraction temperature is 45 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extract is obtained by centrifugation at 4500rpm/min for 13.5 min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.09 mpa and temperature of 60 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 123 ℃, the feeding speed is 25mL/min, and the air suction amount is 70%, so that the common sow thistle extract is obtained.
The result of the detection
The difference detection of the anti-alcoholism and brain-protecting effects of the sonchus oleraceus extract prepared in different extraction times is shown in the following table:
example 10
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 25 deg.C, and the humidity is 15%
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 6, 5% (w/v) choline chloride is added for ultrasonic extraction, the ultrasonic extraction power is 500w, the ultrasonic extraction temperature is 45 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifugation at 4500rpm/min for 13.5 min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.09 mpa and temperature of 60 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 25mL/min, and the air suction amount is 70%, so that the common sow thistle extract is obtained.
Example 11
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 25 deg.C, and the humidity is 15%
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 6, then 10% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 500w, the ultrasonic extraction temperature is 45 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifugation at 4500rpm/min for 13.5 min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.09 mpa and temperature of 60 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 25mL/min, and the air suction amount is 70%, so that the common sow thistle extract is obtained.
Example 12
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 80 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 25 deg.C, and the humidity is 15%
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 6, 15% (w/v) choline chloride is added for ultrasonic extraction, the ultrasonic extraction power is 500w, the ultrasonic extraction temperature is 45 ℃, the ultrasonic extraction time is 20min, and the common sow thistle extract is obtained by centrifugation at 4500rpm/min for 13.5 min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 0.5mpa to obtain filtrate with the molecular weight of less than 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.09 mpa and temperature of 60 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 120 ℃, the feeding rate is 25mL/min, and the air suction amount is 70%, so that the common sow thistle extract is obtained.
The result of the detection
The difference detection of the anti-alcoholism and brain-protecting effects of the sonchus oleraceus extract prepared by different choline chloride adding amounts is shown in the following table:
example 13
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 30 ℃, and the humidity is 20%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 7, then 15% (w/v) choline chloride is added, ultrasonic extraction is carried out, the ultrasonic extraction power is 550w, the ultrasonic extraction temperature is 50 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extracting solution is obtained by centrifugation for 15min at 5000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain a filtrate with the molecular weight of more than 800;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.08 mpa and temperature of 70 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding speed is 30mL/min, and the air extraction amount is 80%, so that the common sow thistle extract is obtained.
Example 14
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 30 ℃, and the humidity is 20%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 7, 15% (w/v) choline chloride is added for ultrasonic extraction, the ultrasonic extraction power is 550w, the ultrasonic extraction temperature is 50 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extract is obtained by centrifugation for 15min at 5000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain filtrate with the molecular weight of 500-800;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.08 mpa and temperature of 70 deg.C;
(5) And (4) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding speed is 30mL/min, and the air extraction amount is 80%, so that the common sow thistle extract is obtained.
Example 15
A method for preparing herba Sonchi arvensis extract with effects of relieving hangover and protecting brain comprises the following steps:
(1) Drying aerial parts of herba Sonchi Oleracei in shade, pulverizing, and sieving with 100 mesh sieve to obtain herba Sonchi Oleracei powder; wherein the drying temperature in the shade is 30 ℃, and the humidity is 20%;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:20 (g.mL-1), the pH value is 7, 15% (w/v) choline chloride is added for ultrasonic extraction, the ultrasonic extraction power is 550w, the ultrasonic extraction temperature is 50 ℃, the ultrasonic extraction time is 40min, and the common sow thistle extract is obtained by centrifugation for 15min at 5000 rpm/min;
(3) Performing membrane filtration on the common sow thistle extracting solution obtained in the step (2) under the pressure of 1.0mpa to obtain a filtrate with the molecular weight less than < 500;
(4) Concentrating the filtrate obtained in step (3) under reduced pressure to obtain concentrated solution with vacuum degree of-0.08 mpa and temperature of 70 deg.C;
(5) And (5) spray-drying the concentrated solution obtained in the step (4), wherein the inlet temperature of spray-drying is 125 ℃, the feeding speed is 30mL/min, and the air suction amount is 80%, so that the common sow thistle extract is obtained.
The result of the detection
The difference detection of the anti-alcoholism and brain-protecting effects of the common sow thistle extracts in different molecular weight ranges is shown in the following table:
in summary, the above examples compare the feed-liquid ratio, the pH of the extraction solution, the extraction time, the choline chloride addition amount, and the molecular weight obtained by membrane filtration, and it is found that the extraction is performed under the conditions of the feed-liquid ratio range of 1
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A preparation method of a common sow thistle extract with the functions of dispelling the effects of alcohol and protecting the brain is characterized by comprising the following steps:
(1) Drying aerial part of herba Sonchi Oleracei in shade, pulverizing, and sieving to obtain herba Sonchi Oleracei powder;
(2) Adding common sow thistle powder into a 50% (v/v) ethanol solution, wherein the ratio of the materials to the liquid is 1:1-1:20 (g.mL-1), pH 5-7, adding 10-15% (w/v) choline chloride, performing ultrasonic extraction, and centrifuging to obtain herba Sonchi arvensis extract;
(3) Membrane filtering the sonchus oleraceus extract obtained in the step (2) to obtain filtrate with molecular weight less than 500;
(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain a concentrated solution;
(5) And (4) spray-drying the concentrated solution obtained in the step (4) to obtain the common sow thistle extract.
2. The method according to claim 1, wherein the temperature of the drying in the shade in step (1) is 20 to 30 ℃ and the humidity is 10 to 20%.
3. The method according to claim 1, wherein the pulverizing and sieving in step (1) is a 60-100 mesh sieve.
4. The method according to claim 1, wherein the power of the ultrasonic extraction in the step (2) is 450-550w, the temperature is 40-50 ℃, and the time is 20-40min.
5. The method according to claim 1, wherein the rotation speed of the centrifugation in the step (2) is 4000-5000rpm/min, and the centrifugation time is 10-15min.
6. The method according to claim 1, wherein the pressure of the membrane filtration in the step (3) is 0.1 to 1.0mpa.
7. The method according to claim 1, wherein the vacuum degree of the vacuum concentration in the step (4) is-0.1 mpa to-0.08 mpa, and the temperature is 50 ℃ to 70 ℃.
8. The method according to claim 1, wherein the inlet temperature of the spray drying in the step (5) is 120 to 125 ℃, the feed rate is 20 to 30mL/min, and the air extraction amount is 60 to 80%.
9. An application of Sonchus oleraceus extract prepared by the preparation method of any one of claims 1-8 in the fields of hangover alleviating, brain protecting and health promoting is provided.
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| CN116474020A (en) * | 2023-05-12 | 2023-07-25 | 北京暄芷达生物科技有限公司 | Sonchus arvensis jujube extract for improving sleep and application thereof |
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| CN108245543A (en) * | 2018-02-06 | 2018-07-06 | 中国农业科学院饲料研究所 | A kind of length splits sonchus oleraceus alkaloid extracting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108245543A (en) * | 2018-02-06 | 2018-07-06 | 中国农业科学院饲料研究所 | A kind of length splits sonchus oleraceus alkaloid extracting method |
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| 高辉等: "超声波辅助提取苦苣总黄酮的工艺研究", 《吉林医药学院学报》, vol. 33, no. 1, pages 20 - 22 * |
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| CN116474020A (en) * | 2023-05-12 | 2023-07-25 | 北京暄芷达生物科技有限公司 | Sonchus arvensis jujube extract for improving sleep and application thereof |
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