CN115607603B - Oral liquid for clearing away plague and toxic materials and preparation method thereof - Google Patents
Oral liquid for clearing away plague and toxic materials and preparation method thereof Download PDFInfo
- Publication number
- CN115607603B CN115607603B CN202211053465.4A CN202211053465A CN115607603B CN 115607603 B CN115607603 B CN 115607603B CN 202211053465 A CN202211053465 A CN 202211053465A CN 115607603 B CN115607603 B CN 115607603B
- Authority
- CN
- China
- Prior art keywords
- parts
- root
- heating
- oral liquid
- plague
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 88
- 206010035148 Plague Diseases 0.000 title claims abstract description 33
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 33
- 239000000463 material Substances 0.000 title claims description 50
- 231100000331 toxic Toxicity 0.000 title claims description 14
- 230000002588 toxic effect Effects 0.000 title claims description 14
- 238000002360 preparation method Methods 0.000 title abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 39
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 21
- 241000037740 Coptis chinensis Species 0.000 claims abstract description 21
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 21
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 21
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 21
- 241001180876 Saposhnikovia Species 0.000 claims abstract description 21
- 239000010440 gypsum Substances 0.000 claims abstract description 21
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 21
- 244000303040 Glycyrrhiza glabra Species 0.000 claims abstract description 20
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 20
- 235000017443 Hedysarum boreale Nutrition 0.000 claims abstract description 20
- 235000007858 Hedysarum occidentale Nutrition 0.000 claims abstract description 20
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 claims abstract description 20
- 235000008599 Poria cocos Nutrition 0.000 claims abstract description 18
- 244000197580 Poria cocos Species 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 239000010231 banlangen Substances 0.000 claims abstract description 16
- 244000246386 Mentha pulegium Species 0.000 claims abstract description 13
- 235000016257 Mentha pulegium Nutrition 0.000 claims abstract description 13
- 235000004357 Mentha x piperita Nutrition 0.000 claims abstract description 13
- 235000001050 hortel pimenta Nutrition 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims description 61
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 45
- 239000003814 drug Substances 0.000 claims description 40
- 239000007787 solid Substances 0.000 claims description 26
- 238000001914 filtration Methods 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 18
- 239000004382 Amylase Substances 0.000 claims description 17
- 108010065511 Amylases Proteins 0.000 claims description 17
- 102000013142 Amylases Human genes 0.000 claims description 17
- 108010059892 Cellulase Proteins 0.000 claims description 17
- 235000019418 amylase Nutrition 0.000 claims description 17
- 229940106157 cellulase Drugs 0.000 claims description 17
- 229940059442 hemicellulase Drugs 0.000 claims description 17
- 108010002430 hemicellulase Proteins 0.000 claims description 17
- 238000002791 soaking Methods 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 13
- 241000334160 Isatis Species 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 11
- 238000001471 micro-filtration Methods 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 10
- 235000006679 Mentha X verticillata Nutrition 0.000 claims description 8
- 235000002899 Mentha suaveolens Nutrition 0.000 claims description 8
- 235000001636 Mentha x rotundifolia Nutrition 0.000 claims description 8
- 239000000919 ceramic Substances 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 5
- 235000002226 Ranunculus ficaria Nutrition 0.000 claims description 4
- 244000081426 Ranunculus ficaria Species 0.000 claims description 4
- 241000050051 Chelone glabra Species 0.000 claims 3
- 239000004480 active ingredient Substances 0.000 abstract description 30
- 238000005728 strengthening Methods 0.000 abstract description 6
- 230000036039 immunity Effects 0.000 abstract description 5
- 210000000952 spleen Anatomy 0.000 abstract description 3
- IPQKDIRUZHOIOM-UHFFFAOYSA-N Oroxin A Natural products OC1C(O)C(O)C(CO)OC1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 IPQKDIRUZHOIOM-UHFFFAOYSA-N 0.000 description 31
- IKIIZLYTISPENI-ZFORQUDYSA-N baicalin Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 IKIIZLYTISPENI-ZFORQUDYSA-N 0.000 description 31
- 229960003321 baicalin Drugs 0.000 description 31
- AQHDANHUMGXSJZ-UHFFFAOYSA-N baicalin Natural products OC1C(O)C(C(O)CO)OC1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 AQHDANHUMGXSJZ-UHFFFAOYSA-N 0.000 description 31
- 108090000790 Enzymes Proteins 0.000 description 19
- 102000004190 Enzymes Human genes 0.000 description 19
- 230000000694 effects Effects 0.000 description 19
- 238000006911 enzymatic reaction Methods 0.000 description 19
- 229940088598 enzyme Drugs 0.000 description 19
- 238000012360 testing method Methods 0.000 description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 10
- 238000004090 dissolution Methods 0.000 description 10
- 241000700605 Viruses Species 0.000 description 8
- 229940079593 drug Drugs 0.000 description 8
- 239000000284 extract Substances 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000001976 improved effect Effects 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000003053 toxin Substances 0.000 description 5
- 231100000765 toxin Toxicity 0.000 description 5
- 241000282887 Suidae Species 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- 238000010298 pulverizing process Methods 0.000 description 4
- 238000010926 purge Methods 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 241000218202 Coptis Species 0.000 description 3
- 235000002991 Coptis groenlandica Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 206010019233 Headaches Diseases 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- 208000002193 Pain Diseases 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 208000025865 Ulcer Diseases 0.000 description 3
- 229930013930 alkaloid Natural products 0.000 description 3
- 150000003797 alkaloid derivatives Chemical class 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 231100000869 headache Toxicity 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 208000011580 syndromic disease Diseases 0.000 description 3
- 230000035922 thirst Effects 0.000 description 3
- 231100000397 ulcer Toxicity 0.000 description 3
- 241000712461 unidentified influenza virus Species 0.000 description 3
- 208000006820 Arthralgia Diseases 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 208000001431 Psychomotor Agitation Diseases 0.000 description 2
- 241000725643 Respiratory syncytial virus Species 0.000 description 2
- 206010038743 Restlessness Diseases 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 230000002155 anti-virotic effect Effects 0.000 description 2
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000001671 coumarin Nutrition 0.000 description 2
- 125000000332 coumarinyl group Chemical class O1C(=O)C(=CC2=CC=CC=C12)* 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 201000009240 nasopharyngitis Diseases 0.000 description 2
- 238000012803 optimization experiment Methods 0.000 description 2
- -1 phenylpropanoid glycosides Chemical class 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 1
- 206010007882 Cellulitis Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 241000037803 Coptis deltoidea Species 0.000 description 1
- 206010012218 Delirium Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010013789 Dry throat Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 208000037487 Endotoxemia Diseases 0.000 description 1
- 241000576429 Forsythia suspensa Species 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
- 244000111489 Gardenia augusta Species 0.000 description 1
- 235000018958 Gardenia augusta Nutrition 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 206010019345 Heat stroke Diseases 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241000589902 Leptospira Species 0.000 description 1
- 206010026749 Mania Diseases 0.000 description 1
- 241000972672 Phellodendron Species 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 241001619461 Poria <basidiomycete fungus> Species 0.000 description 1
- 208000027954 Poultry disease Diseases 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 241000405414 Rehmannia Species 0.000 description 1
- 240000004534 Scutellaria baicalensis Species 0.000 description 1
- 235000017089 Scutellaria baicalensis Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 206010043376 Tetanus Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 208000003443 Unconsciousness Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 description 1
- 229940093265 berberine Drugs 0.000 description 1
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 description 1
- 230000004856 capillary permeability Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000000916 dilatatory effect Effects 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 150000004141 diterpene derivatives Chemical class 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000000531 effect on virus Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- FPJQGFLUORYYPE-UHFFFAOYSA-N epiberberine Chemical compound C1=C2C=C(C3=C(C=C(C(=C3)OC)OC)CC3)[N+]3=CC2=C2OCOC2=C1 FPJQGFLUORYYPE-UHFFFAOYSA-N 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 206010018388 glossodynia Diseases 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930003658 monoterpene Natural products 0.000 description 1
- 150000002773 monoterpene derivatives Chemical class 0.000 description 1
- 235000002577 monoterpenes Nutrition 0.000 description 1
- 208000018962 mouth sore Diseases 0.000 description 1
- 208000004296 neuralgia Diseases 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 208000004371 toothache Diseases 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/238—Saposhnikovia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/534—Mentha (mint)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/808—Scrophularia (figwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Diabetes (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Hematology (AREA)
- Nutrition Science (AREA)
- Neurosurgery (AREA)
- Obesity (AREA)
- Rheumatology (AREA)
- Virology (AREA)
- Inorganic Chemistry (AREA)
- Pain & Pain Management (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an oral liquid for clearing plague and detoxifying and a preparation method thereof, and the oral liquid comprises the following raw materials in parts by weight: 200-400 parts of coptis chinensis, 250-350 parts of radix isatidis, 180-220 parts of divaricate saposhnikovia root, 200-250 parts of radix scutellariae, 100-150 parts of honeysuckle, 130-170 parts of gypsum, 80-120 parts of poria cocos, 50-80 parts of honey-fried licorice root, 120-180 parts of radix scrophulariae, 5-10 parts of lotus seed and 5-10 parts of peppermint. The prescription of the invention has reasonable compatibility of the prescription and cooperatively plays roles of clearing heat and detoxicating, strengthening body resistance and strengthening the body, replenishing qi and strengthening the spleen and improving the immunity of organisms. Meanwhile, the preparation method provided by the invention can improve the content of active ingredients in the composition, and improve the extraction efficiency and the product quality.
Description
Technical Field
The invention belongs to the technical field of veterinary medicines, and in particular relates to a pestilence-clearing and detoxifying oral liquid and a preparation method thereof.
Background
The oral liquid for clearing away the plague and detoxifying is a traditional Chinese veterinary medicine compound preparation which is most commonly used in prevention and control of poultry diseases in recent years, has the efficacy of clearing away the heat-evil and expelling superficial evils and mainly treats exogenous fever of the poultry. The prescription consists of radix isatidis, radix scutellariae, fructus forsythiae, fructus gardeniae, radix scrophulariae, radix rehmanniae and other medicinal materials. Traditional Chinese medicine suggests that fowl biological diseases are usually caused by invasion of heat toxin from shallow to deep, from exterior to interior and from heart lung to lower body, and the disease is caused by excessive heat evil in qi system, and the heat evil in blood system is easy to damage body fluid, so animals are ill: the general manifestations are: thirst, difficulty in acting, and stiffness of the extremities. The morbidity of animals is mainly acute, and the mortality rate is relatively high. Radix Isatidis in the oral liquid prescription for clearing away heat and toxic materials can detoxify and moisten throat; radix Scutellariae has effects of clearing lung-heat and purging heat; fructus forsythiae has effects of clearing heat and resisting bacteria, and eliminating upper middle-upper coke gas; fructus Gardeniae is used as adjuvant medicine for eliminating triple energizer heat syndrome, purging pathogenic fire and removing toxic substances; the brown participation of Sheng Di Huang can cool blood and reduce fire, thereby making up the kidney, and being an auxiliary drug. Clinical application in many countries proves that the preparation has obvious curative effect on prevention and control of poultry virus and germ, but the traditional production process has the defects of low extraction rate of medicinal material active ingredients, high loss in impurity removal and concentration links, high energy efficiency and the like. Meanwhile, due to the long-term use of various chemicals, antibiotics and the like, animals are extremely easy to generate drug resistance, although the dosage is continuously increased, the curative effect is not obviously improved or even continuously reduced.
For example, the traditional process generally has the defects of low dissolution rate of active ingredients extracted from decoction pieces, high loss rate of active ingredients except heterocyclic joints, unstable thermosensitive ingredients in a concentrating link, high loss rate of ingredients and the like, and also has the defects of high resource loss, high energy consumption, serious environmental pollution, high production cost and the like, so that the clinical and efficient exertion and the market competitiveness of the prescription are limited.
Therefore, how to innovate and improve the formulation of the oral liquid for clearing plague, and change the traditional preparation process, so as to provide a novel preparation process of the veterinary drug with simple and convenient process, high active ingredient yield, short extraction time, energy conservation and environmental protection, so as to enhance the exertion of clinical efficacy, which is a problem to be solved urgently.
Disclosure of Invention
The invention aims to provide an oral liquid for clearing away plague and toxic materials and a preparation method thereof, which can solve the problem of poor curative effect of the existing oral liquid and improve the dissolution rate of active ingredients of medicinal materials.
The invention provides an oral liquid for clearing plague and detoxicating, which comprises the following raw materials in parts by weight: 200-400 parts of coptis chinensis, 250-350 parts of radix isatidis, 180-220 parts of divaricate saposhnikovia root, 200-250 parts of radix scutellariae, 100-150 parts of honeysuckle, 130-170 parts of gypsum, 80-120 parts of poria cocos, 50-80 parts of honey-fried licorice root, 120-180 parts of radix scrophulariae, 5-10 parts of lotus seed and 5-10 parts of peppermint.
Further, the material comprises the following raw materials in parts by weight: 250-350 parts of coptis chinensis, 300-320 parts of radix isatidis, 200-210 parts of divaricate saposhnikovia root, 220-230 parts of radix scutellariae, 120-140 parts of honeysuckle, 130-150 parts of gypsum, 90-100 parts of poria cocos, 50-60 parts of honey-fried licorice root, 140-150 parts of radix scrophulariae, 5-8 parts of lotus seeds and 5-8 parts of mint.
Further, the material comprises the following raw materials in parts by weight: 300 parts of coptis chinensis, 300 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 200 parts of radix scutellariae, 120 parts of honeysuckle, 150 parts of gypsum, 90 parts of poria cocos, 60 parts of honey-fried licorice root, 150 parts of radix scrophulariae, 8 parts of lotus seeds and 5 parts of mint.
The invention also provides a preparation method of the oral liquid for clearing plague and detoxifying, which comprises the following steps:
(1) Mixing the components in proportion, crushing to 100-200 meshes, and then adding water with the weight 10-20 times of the weight of the mixture for heating and soaking;
(2) Filtering the mixture soaked in the step (1), heating by microwaves, heating to 60 ℃ firstly, then keeping for 10-30min, heating to 70 ℃ again, then keeping for 10-30min, and finally heating to 90 ℃ and keeping for 20-40min; separating the liquid and the solid;
(3) Soaking the solid in water, adjusting pH to 5.0, and adding cellulase, hemicellulase, pectase and amylase for enzymolysis;
(4) Heating to extract liquid medicine after enzymolysis, mixing the liquid medicine with the liquid separated in the step (2), decocting for 3-5 times, mixing the decoctions, centrifuging, and micro-filtering the supernatant.
Further, the heating temperature in the step (1) is 35-45 ℃, and the soaking time is 60-120min.
Further, in the step (3), the weight of the added water is 5-8 times of that of the solid, the mass ratio of the cellulase to the hemicellulase to the pectinase to the amylase is 1-2:1:1:3-5, the enzymolysis time is 30-40min, and the enzymolysis temperature is 35-45 ℃.
Further, the final temperature of the temperature rise in the step (4) is 75-85 ℃, and the extraction time is 45-55min.
Further, the step of microfiltration in the step (4) specifically includes: and (3) circularly micro-filtering the supernatant by using an inorganic ceramic membrane micro-filter in a cross-flow mode, adding distilled water with the amount of 2 times of that of the medicinal materials, continuing micro-filtering, and concentrating the micro-filtered liquid.
Further, the parameters of the microfiltration include: the aperture of the inorganic ceramic membrane is 0.2 mu m, the flow rate of the feed is 30-50m/s, and the concentration multiple is 3-5 times.
The Chinese medicinal materials in the invention comprise:
the modern pharmacological experiment research proves that the radix scutellariae contains a large amount of Huang Tongyan substances, so that the radix scutellariae has various effects of resisting inflammation, clearing heat and detoxicating, and the like, and has certain inhibition effects on the aspects of resisting virus, resisting tumor, resisting inflammation, resisting bacteria, protecting neuron cells, and the like. The in vitro experiment of locust proves that after decocting the radix scutellariae with water, the extract is taken to inhibit the reproduction of leptospira, the concentrated extract of the radix scutellariae in water has direct killing effect on some pathogenic microorganisms in human body, the radix scutellariae also has strong inhibiting and killing effect on some viruses, and the radix scutellariae also has strong inhibiting effect on respiratory viruses. The radix Scutellariae contains a large amount of coumarins, which are the main substances for inhibiting respiratory syncytial virus, and the radix Scutellariae extract contains a large amount of coumarins. Meanwhile, the radix scutellariae extract has a certain elimination effect on viruses such as respiratory syncytial virus, influenza virus and hepatitis B virus.
The isatis root contains a large amount of polysaccharide, starch, amino acid and other components, and in terms of antivirus, the water extract of the isatis root, the active component extract in the isatis root and the like are found to have stronger antivirus effect, and the alkaloid extract in the isatis root has disinfection and sterilization effects on influenza viruses. And the polysaccharide captured by the radix isatidis can strengthen the body fluid immunity function and the nonspecific immunity function of poultry and improve the capability of animals for resisting influenza viruses.
The radix scrophulariae has complex chemical components and mainly contains alkaloid, iridoids, volatile oil, phytosterol, phenylpropanoid glycosides, flavonoids, saccharides, organic acids, trace amount of monoterpene and diterpene, etc. The radix scrophulariae polysaccharide extract can obviously reduce blood lactic acid and serum urea content and increase liver glycogen content. In addition, radix scrophulariae has effects of dilating coronary artery, improving microcirculation and capillary permeability, improving endotoxemia, relieving pain, tranquilizing, and resisting convulsion.
Rhizoma Coptidis is rhizome of Coptidis rhizoma of Coptis genus of Ranunculaceae family, coptis deltoidea leaf or Coptis yunnanensis; contains alkaloid such as berberine, epiberberine, and phellodendron ketone; bitter and cold in nature and enters heart, liver, stomach and large intestine meridians; has effects in clearing away heat and toxic materials, purging pathogenic fire, and eliminating dampness; is mainly used for treating heat toxin sores and ulcers, furuncle, swelling and pain of gum, mouth and tongue sores and the like; modern pharmacology proves that coptis chinensis has the effects of resisting microorganisms, resisting protozoa, viruses, arrhythmia, ulcer, tumor, inflammation and the like.
Radix Saposhnikoviae has effects of inducing sweat, eliminating phlegm, dispelling pathogenic wind, relieving exterior syndrome, and relieving pain, and can be used for treating common cold, headache, joint pain, neuralgia, etc. Fang Feng is used as root drug. Pungent and sweet in flavor and warm in nature. Has effects in relieving exterior syndrome, inducing perspiration, dispelling pathogenic wind, and removing dampness, and can be used for treating common cold due to wind-cold, headache, fever, joint pain, and tetanus; in addition, the wind-proof blade and the wind-proof flower can also be used for medicine.
Gypsum, mainly: muscle-relaxing and heat-clearing; relieving restlessness and quenching thirst. The main fever is strong and the heat does not return; restlessness and unconsciousness; delirium and mania; thirst and dry throat; dyspnea due to lung heat; heatstroke spontaneous perspiration; headache due to stomach fire; toothache due to stomach fire; excessive heat toxin; spot eruption; sores of the mouth and tongue; carbuncle, cellulitis and sore and ulcer; the mouth is not closed; soup fire scalds. Channel tropism: enter lung and stomach meridians.
In summary, the invention has the following advantages:
1. the invention adopts isatis root, baikal skullcap root, coptis root and divaricate saposhnikovia root as principal drugs, honeysuckle flower and figwort root as ministerial drugs, lotus seed and peppermint as adjuvant drugs, gypsum for clearing heat and purging fire, poria for enhancing immunity, and honey-fried licorice root for harmonizing the drugs, thereby not only maintaining the efficacy of strong fire in original place, but also overcoming the defect of insufficient antiviral capability, and achieving the purposes of clearing heat and expelling toxin, activating blood and dissolving stasis, nourishing yin and cooling blood, replenishing qi and strengthening spleen and treating both principal and secondary aspect of disease.
2. The oral liquid for clearing away the plague and toxic materials has reasonable formula, has the functions of resisting bacteria and viruses, strengthening body resistance, clearing away heat and toxic materials, enriching blood and activating blood, replenishing qi and strengthening spleen, stimulating appetite, promoting growth and improving body immunity, has unique curative effect on the plague and toxic materials of livestock, and has obvious curative effect on various diseases of livestock caused by viruses or bacteria.
3. In the preparation method provided by the invention, microwave three-section heating is adopted, the traditional Chinese medicine can be fully soaked to fully swell cells, the cell internal pressure is rapidly increased under the action of microwave high frequency, the cell wall is broken, and the internal active ingredients are released; then carrying out enzymolysis treatment on plant cells containing cell walls, and further destroying the cell walls and improving the dissolution rate of active ingredients in the medicinal materials by optimizing the compound enzyme with the proportion relation; the combined liquid medicine is decocted again, and the yield of the baicalin can be obviously improved by a microfiltration method, so that the loss of main active ingredients in the impurity removal process is avoided.
Detailed Description
The principles and features of the present invention are described below in connection with the following examples, which are set forth to illustrate, but are not to be construed as limiting the scope of the invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1
The embodiment provides an oral liquid for clearing plague and detoxifying, which comprises the following raw materials in parts by weight: 300 parts of coptis chinensis, 300 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 200 parts of radix scutellariae, 120 parts of honeysuckle, 150 parts of gypsum, 90 parts of poria cocos, 60 parts of honey-fried licorice root, 150 parts of radix scrophulariae, 8 parts of lotus seeds and 5 parts of mint.
The embodiment also provides a preparation method of the oral liquid for clearing plague and detoxifying, which comprises the following steps:
(1) Mixing the components in proportion, pulverizing to 100 meshes, adding water 10 times of the weight of the mixture, heating to 35 ℃, and soaking for 60min;
(2) Filtering the mixture soaked in the step (1), heating by microwaves, heating to 60 ℃ firstly, then keeping for 20min, heating to 70 ℃ again, then keeping for 20min, and finally heating to 90 ℃ and keeping for 20min; separating the liquid and the solid;
(3) Soaking the solid in water with the mass of 5 times of that of the solid, adjusting the pH to 5.0, and adding cellulase, hemicellulase, pectase and amylase accounting for 0.1% of the mass of the solid for enzymolysis at 45 ℃; the mass ratio of the cellulase to the hemicellulase to the pectase to the amylase is 1:1:1:3, the enzymolysis time is 30min, and the enzymolysis temperature is 45 ℃;
(4) Heating to 80 ℃ after enzymolysis to extract liquid medicine for 50min, mixing the liquid medicine with the liquid collected in the step (2), decocting for 3 times, combining the decoction, centrifuging, and micro-filtering the supernatant to obtain the product;
the step of microfiltration specifically comprises: and (3) circularly micro-filtering the supernatant by using an inorganic ceramic membrane micro-filter with a membrane aperture of 0.2 mu m in a cross-flow mode, wherein the flow rate of the feed is 30m/s, adding 2 times of distilled water into the medicinal materials for continuous micro-filtration, and concentrating the micro-filtered liquid for 3 times.
Example 2
The embodiment provides an oral liquid for clearing plague and detoxifying, which comprises the following components in parts by weight:
200 parts of coptis chinensis, 250 parts of radix isatidis, 180 parts of divaricate saposhnikovia root, 200 parts of radix scutellariae, 100 parts of honeysuckle, 130 parts of gypsum, 80 parts of poria cocos, 50 parts of honey-fried licorice root, 120 parts of radix scrophulariae, 5 parts of lotus seed and 5 parts of mint.
The embodiment also provides a preparation method of the oral liquid for clearing plague and detoxifying, which comprises the following steps:
(1) Mixing the components in proportion, pulverizing to 200 meshes, adding water with the weight 20 times of that of the mixture, heating to 35 ℃, and soaking for 120min;
(2) Filtering the mixture soaked in the step (1), heating by microwaves, heating to 60 ℃ firstly, then keeping for 30min, heating to 70 ℃ again, then keeping for 10min, and finally heating to 90 ℃ and keeping for 20min; collecting liquids and solids;
(3) Soaking the solid in water with the mass of 8 times of that of the solid, adjusting the pH to 5.0, and adding cellulase, hemicellulase, pectase and amylase accounting for 0.1% of the mass of the solid for enzymolysis at 45 ℃; the mass ratio of the cellulase to the hemicellulase to the pectase to the amylase is 2:1:1:5, and the enzymolysis time is 30min;
(4) Heating to 85 ℃ after enzymolysis to extract liquid medicine for 45min, mixing the liquid medicine with the liquid collected in the step (2), decocting for 3 times, combining the decoction, centrifuging, and micro-filtering the supernatant to obtain the product;
the step of microfiltration specifically comprises: and (3) circularly micro-filtering the supernatant by using an inorganic ceramic membrane micro-filter with a membrane aperture of 0.2 mu m in a cross-flow mode, wherein the flow rate of the feed is 30m/s, adding 2 times of distilled water into the medicinal materials for continuous micro-filtration, and concentrating the micro-filtered liquid for 3 times.
Example 3
The embodiment provides an oral liquid for clearing plague and detoxifying, which comprises the following components in parts by weight:
400 parts of coptis chinensis, 350 parts of isatis root, 220 parts of divaricate saposhnikovia root, 20 parts of radix scutellariae, 150 parts of honeysuckle, 170 parts of gypsum, 120 parts of poria cocos, 80 parts of honey-fried licorice root, 180 parts of radix scrophulariae, 10 parts of lotus seed and 10 parts of mint.
The embodiment also provides a preparation method of the oral liquid for clearing plague and detoxifying, which comprises the following steps:
(1) Mixing the components in proportion, pulverizing to 200 meshes, adding water 10 times of the weight of the mixture, heating to 45 ℃, and soaking for 60min;
(2) Filtering the mixture soaked in the step (1), heating by microwaves, heating to 60 ℃ firstly, then keeping for 30min, heating to 70 ℃ again, then keeping for 30min, and finally heating to 90 ℃ and keeping for 20min; collecting liquids and solids;
(3) Soaking the solid in water with the mass of 8 times of that of the solid, adjusting the pH to 5.0, and adding cellulase, hemicellulase, pectase and amylase accounting for 0.1% of the mass of the solid for enzymolysis at 45 ℃; the mass ratio of the cellulase to the hemicellulase to the pectase to the amylase is 2:1:1:3, and the enzymolysis time is 30min;
(4) Heating to 85 ℃ after enzymolysis to extract liquid medicine for 45min, mixing the liquid medicine with the liquid collected in the step (2), decocting for 3 times, combining the decoction, centrifuging, and micro-filtering the supernatant to obtain the product;
the step of microfiltration specifically comprises: and (3) circularly micro-filtering the supernatant by using an inorganic ceramic membrane micro-filter with a membrane aperture of 0.2 mu m in a cross-flow mode, wherein the feeding flow rate is 50m/s, adding distilled water with the amount of 2 times that of the medicinal materials for continuous micro-filtration, and concentrating the micro-filtered liquid for 3 times to obtain the medicine.
Comparative example 1
300 parts of coptis chinensis, 200 parts of divaricate saposhnikovia root, 200 parts of radix scutellariae, 120 parts of honeysuckle, 90 parts of poria cocos, 60 parts of honey-fried licorice root, 150 parts of radix scrophulariae, 8 parts of lotus seeds and 5 parts of mint.
This comparative example was prepared in the same manner as in example 1.
Comparative example 2
300 parts of coptis chinensis, 300 parts of isatis root, 200 parts of divaricate saposhnikovia root, 120 parts of honeysuckle, 150 parts of gypsum, 90 parts of poria cocos, 150 parts of radix scrophulariae, 8 parts of lotus seed and 5 parts of mint.
This comparative example was prepared in the same manner as in example 1.
Comparative example 3
The preparation method of the oral liquid for clearing plague and detoxicating is as follows in the second book of national standard of local standard liter of veterinary medicine:
taking 1000mL of oral liquid as an example, decocting 150g of rehmannia root, 250g of cape jasmine fruit, 225g of radix scutellariae, 200g of weeping forsythia, 150g of radix scrophulariae and 200g of radix isatidis with water twice, mixing the decoctions, standing, filtering, concentrating, adding a proper amount of sodium benzoate, adding water to 1000mL, filling and sterilizing.
Test example 1
The oral liquid for clearing away the plague and expelling the toxin prepared in examples 1-3 and comparative examples 1-3 was studied for its drug properties.
1.1 clinical trial:
1.1.1 laboratory animals: pigs are 300 pigs at 6-10 months of age and all suffer from pestilence and are randomly divided into 6 groups of 50 pigs each, namely three experimental groups and three control groups.
1.1.2 administration: the experimental group and the control group correspond to the oral liquid prepared in the examples 1-3 and the comparative examples 1-3 respectively. Every 1000mL of the oral liquid is added with 25kg of water, and the oral liquid is fed in drinking water for 5 days as one period and for 2 periods.
1.1.3 effect judgment:
(1) Decision criterion
And (3) curing: the pig has good status and strong ingestion ability.
Invalidation: the symptoms were not alleviated.
1.2 experimental results
After 2 cycles, the results of 300 pigs with disease are shown in table 1:
table 1 experimental results
Cure only a few | Invalid count | |
Example 1 | 49 | 1 |
Example 2 | 48 | 2 |
Example 3 | 48 | 2 |
Comparative example 1 | 35 | 15 |
Comparative example 2 | 40 | 10 |
Comparative example 3 | 37 | 13 |
As shown in Table 1, the oral liquid for clearing away the plague and toxic materials prepared by the invention has unique curative effect and obvious curative effect on various diseases of livestock and poultry caused by viruses or bacteria.
Test example 2
The content detection of baicalin in the following test examples is a high performance liquid chromatography detection method, and comprises the following steps:
sample preparation: taking 1mL of the liquid medicine, placing in a 50mL volumetric flask, adding a proper amount of 50% methanol solution, performing ultrasonic treatment for 20min (100W, 40 KHz), standing to room temperature, fixing the volume to a scale with the 50% methanol solution, shaking uniformly, and filtering with a microporous filter membrane (0.45 μm).
Chromatographic conditions: kromasil C18 (250 mm. Times.4.6 mm,5 μm) was used as column, octadecylsilane chemically bonded silica (C18) was used as filler, and acetonitrile was used as mobile phase: 0.2% phosphoric acid solution (22:78).
The detection wavelength is 274nm, the flow rate is 1.0mL/min, the column temperature is 40 ℃, and the sample injection amount is 10 mu L.
The optimization experiment of the crushing and extraction process is carried out, and the experimental example carries out the research of the crushing pretreatment process on the medicinal materials of the prescription of the oral liquid for clearing plague and detoxicating, and comprehensively compares the influence of different crushing and extraction parameters on the yield of active ingredients of the medicinal liquid.
The method specifically comprises the following steps:
2.1 test methods
2.1.1 particle size of the crushed material
300g of coptis chinensis, 300g of isatis root, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seeds and 5g of peppermint are respectively and evenly mixed after being crushed, and are respectively screened by a 50-mesh sieve, a 80-mesh sieve, a 100-mesh sieve, a 120-mesh sieve and a 200-mesh sieve, 10 times of water is added, and the rest steps are the same as in example 1, and the content of baicalin is measured by a high performance liquid chromatograph after the oral liquid for clearing plague and detoxicating is prepared.
2.1.2 heating time
300g of coptis chinensis, 300g of isatis root, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seeds and 5g of peppermint are respectively and evenly mixed after being crushed, screened and sieved by a 100-mesh sieve, 10 times of water is added, soaked for 60min, then directly heated to 90 ℃ for extraction for 30min, 40min, 50min and 60min respectively, and the method provided in the embodiment 1 is adopted, firstly heated to 60 ℃, kept for 20min, then heated to 70 ℃ for 20min, finally heated to 90 ℃ and kept for 20min, after extraction is finished, other steps are the same as those in the embodiment 1, and after the oral liquid for clearing plague and detoxifying is prepared, the content of baicalin is measured by a high-performance liquid chromatograph.
2.1.3 extraction temperature
300g of coptis chinensis, 300g of isatis root, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seeds and 5g of peppermint are respectively and evenly mixed, screened and sieved by a 100-mesh sieve, 10 times of water is added, soaked for 60min, then respectively extracted for 60min at 60 ℃ and 70 ℃ and 90 ℃, and the method provided in the embodiment 1 is that the temperature is firstly heated to 60 ℃ and then kept for 20min, then heated to 70 ℃ and then kept for 20min, after the extraction is finished, the rest steps are the same as those in the embodiment 1, and the content of the baicalin is measured by a high-performance liquid chromatograph after the oral liquid for clearing plague and detoxification is prepared.
2.1.4 Water addition
300g of coptis chinensis, 300g of isatis root, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seeds and 5g of peppermint are crushed, uniformly mixed, screened and screened by a 100-mesh sieve, 200g of each part is respectively added with 600mL, 1000mL, 1600mL, 2000mL and 3000mL of water, soaked for 60min, and after extraction, the rest steps are the same as in example 1, and the content of baicalin is measured by a high-performance liquid chromatograph after the oral liquid for clearing plague and detoxicating is prepared.
2.2 test results
2.2.1 particle size of the crushed material
The yield of the active ingredients of the traditional Chinese medicinal materials is closely related to whether the traditional Chinese medicinal materials are crushed and extracted or not, the crushing meshes of the medicinal materials are different, and the yields of the active ingredients are also different, which is probably caused by the difference of the contact areas of the solvent and the medicinal materials. As the number of pulverized medicinal materials increases, the dissolution rate of active ingredients increases, because the solvent can be more fully contacted with the medicinal materials. The test results show that: when the crushing fineness is 100 meshes, the baicalin yield in the liquid medicine reaches the maximum value, but the granularity of the medicinal materials is too small, so that the problem of removing impurities in the later period is solved, and after comprehensive comparison, the crushing fineness of the medicinal materials is selected to be better than that of the medicinal materials passing through a 100-mesh sieve. The test results are shown in table 2:
TABLE 2 influence of crushing particle size on baicalin content
Crushing granularity (mesh) | 50 | 80 | 100 | 120 | 200 |
Content (mg/mL) | 6.79 | 6.95 | 6.98 | 6.82 | 6.55 |
2.2.2 extraction time
The extraction time in the extraction process of the traditional Chinese medicine is closely related to the yield of the active ingredients, and the longer the extraction time is, the longer the solvent is contacted with the medicine, the larger the dissolution rate of the active ingredients is, because the solvent can be contacted with the medicine more fully. Meanwhile, the cell walls can be broken gradually by adopting stage heating, the dissolution efficiency is improved, and the test result is obtained: when the extraction time is 60min and the temperature is raised in stages, the baicalin content of the obtained liquid medicine reaches the maximum value, and the test results are shown in the following table 3:
TABLE 3 influence of extraction time on baicalin content
Extraction time (min) | 30 | 40 | 50 | 60 | Example 1 |
Content (mg/mL) | 5.23 | 5.65 | 6.04 | 6.65 | 6.98 |
2.2.3 extraction temperature
The yield of the active ingredients extracted from the traditional Chinese medicine is closely related to the extraction time and the extraction temperature, and the more the extraction time is sufficient, the more the active ingredients are dissolved out at a certain temperature. Meanwhile, as the extraction temperature is increased, the dissolution rate of the active ingredients is gradually increased, because the higher the temperature is, the more active ingredients in the medicinal materials move more severely and are more easily dissolved. Meanwhile, when the temperature is raised to 80-90 ℃, the content change is not obvious, but a certain dissolution rate can be improved by adopting a stage temperature raising method, so that the test results show that: when the temperature reaches above 90 ℃, the yield of the active ingredient tends to be balanced, so that the extraction temperature of 90 ℃ is selected to be better. As shown in table 4 below:
TABLE 4 influence of extraction temperature on baicalin content
Extraction temperature (. Degree. C.) | 60 | 70 | 80 | 90 | Example 1 |
Content (mg/mL) | 5.69 | 5.88 | 6.44 | 6.65 | 6.98 |
2.2.4 Water addition
In the extraction process of the traditional Chinese medicinal materials, the water adding amount is extremely related to the yield of the active ingredients, and researches prove that the yield of the active ingredients is gradually increased along with the increase of the water adding amount, and the more the solvent amount is, the more the solvent amount is in full contact with the medicinal materials, so that the active ingredients are more soluble. As can be seen from the data shown in table 5 below, when the water addition amount reaches 10 times or more, the yield of the active ingredient tends to be maximum, and the factors such as energy consumption are comprehensively considered, and the test results show that: the water adding amount is 10 times of that of the medicinal materials, and is better.
TABLE 5 influence of the amount of added water on the baicalin content
Water addition multiple (mL) | 600 | 1000 | 1600 | 2000 | 3000 |
Content (mg/mL) | 4.98 | 5.24 | 5.68 | 6.15 | 6.23 |
The research shows that when plant cells are broken, the fiber structure is destroyed, the effective components in the cells can be fully exposed, and then the effective components are directly contacted with the organic reagent for extraction and separation, which shortens the extraction and separation time greatly compared with the extraction of Chinese medicinal decoction pieces, thereby improving the dissolution rate of the Chinese medicaments and reducing the resource loss.
In summary, the optimal technical parameters of the crushing and extracting process of the invention are as follows: pulverizing the medicinal materials, sieving with 100 mesh sieve, extracting for 60min with three-stage heating, extracting at 90deg.C with water amount 10 times of that of the medicinal materials. Under the condition of the technological parameters, the yield of the active ingredients in the obtained liquid medicine is highest.
Test example 3
The optimization experiment of the biological enzyme extraction process is carried out, and the experiment example comprehensively compares the influence of the type and the dosage of the biological enzyme on the yield of the active ingredients of the liquid medicine. The experimental example utilizes the principle of enzymolysis wall breaking of plant cell walls by biological enzymes, selects four enzymes of cellulase, hemicellulase, pectase, amylase and the like to form compound enzyme according to the characteristics of the medicinal ingredients of the composition, and screens out the optimal enzyme dosage and optimal enzyme reaction conditions.
3.1 test methods
3.1.1 enzyme dosage
300g of coptis chinensis, 300g of radix isatidis, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seed and 5g of peppermint are crushed and screened by a 100-mesh sieve for standby. Heating purified water 10 times of the weight of the medicinal materials to 35 ℃, and soaking for 60min at constant temperature; filtering the soaked mixture, heating by microwaves, heating to 60 ℃ firstly, then keeping for 20min, heating to 70 ℃ again, then keeping for 20min, and finally heating to 90 ℃ and keeping for 20min; collecting liquids and solids;
introducing condensed water into the solid, regulating the pH of the liquid medicine to 5.0 by using 40% citric acid aqueous solution, respectively adding cellulase, hemicellulase, pectase and amylase which are 0.1% in total, and designing the following different proportions for experiments: 1:0.5:1:5, 1:1:2:2, 1:1:2.5:1 and 1:1:1:3, stirring uniformly, performing enzymolysis at 45 ℃ for 30min, performing the rest steps in the same way as in example 1, and checking the content of baicalin after preparing the oral liquid for clearing plague and detoxicating.
3.1.2 enzyme reaction time
300g of coptis chinensis, 300g of radix isatidis, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seed and 5g of peppermint are crushed and screened by a 100-mesh sieve for standby. Heating purified water 10 times of the weight of the medicinal materials to 35 ℃, and soaking for 60 minutes at constant temperature; filtering the soaked mixture, heating by microwaves, heating to 60 ℃ firstly, then keeping for 20min, heating to 70 ℃ again, then keeping for 20min, and finally heating to 90 ℃ and keeping for 20min; collecting liquids and solids;
introducing condensed water into the solid, regulating the pH of the liquid medicine to 5.0 by using 40% citric acid aqueous solution, adding cellulase, hemicellulase, pectase and amylase with the mass ratio of 1:1:1:3, respectively carrying out enzymolysis for 10, 20, 30 and 60min at 45 ℃, and then heating to 80 ℃ for enzyme inactivation treatment; extracting for 50min, and checking the content of baicalin after preparing the oral liquid for clearing plague and detoxicating by the rest steps as in the example 1.
3.1.3 optimal temperature for enzymatic reaction
300g of coptis chinensis, 300g of radix isatidis, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seed and 5g of peppermint are crushed and screened by a 100-mesh sieve for standby. Heating purified water 10 times of the weight of the medicinal materials to 35 ℃, and soaking for 60 minutes at constant temperature; filtering the soaked mixture, heating by microwaves, heating to 60 ℃ firstly, then keeping for 20min, heating to 70 ℃ again, then keeping for 20min, and finally heating to 90 ℃ and keeping for 20min; collecting liquids and solids;
introducing condensed water into the solid, regulating the pH of the liquid medicine to 5.0 by using 40% citric acid aqueous solution, adding cellulase, hemicellulase, pectase and amylase with the mass ratio of 1:1:1:3, respectively carrying out enzymolysis at 20, 30, 40 and 45 ℃ for 30min, and then heating to 80 ℃ for enzyme inactivation treatment; extracting for 50min, and checking the content of baicalin after preparing the oral liquid for clearing plague and detoxicating by the rest steps as in the example 1.
3.1.4 optimal pH for enzymatic reaction
300g of coptis chinensis, 300g of radix isatidis, 200g of divaricate saposhnikovia root, 200g of radix scutellariae, 120g of honeysuckle, 150g of gypsum, 90g of poria cocos, 60g of honey-fried licorice root, 150g of radix scrophulariae, 8g of lotus seed and 5g of peppermint are crushed and screened by a 100-mesh sieve for standby. Heating purified water 10 times of the weight of the medicinal materials to 35 ℃, and soaking for 60 minutes at constant temperature; filtering the soaked mixture, heating by microwaves, heating to 60 ℃ firstly, then keeping for 20min, heating to 70 ℃ again, then keeping for 20min, and finally heating to 90 ℃ and keeping for 20min; collecting liquids and solids;
introducing condensed water into the solid, regulating the pH of the liquid medicine to 4.0, 4.5, 5.0 and 5.5 by using 40% citric acid aqueous solution, adding cellulase, hemicellulase, pectase and amylase in a mass ratio of 1:1:1:3, carrying out enzymolysis for 30min at 45 ℃, and then heating to 80 ℃ for enzyme inactivation treatment; extracting for 50min, and checking the content of baicalin after preparing the oral liquid for clearing plague and detoxicating by the rest steps as in the example 1.
3.2 experimental results
3.2.1 enzyme dosage
The content of baicalin is detected by HPLC, and the detection results are shown in the following table, and the data statistics result shows that when the total content of cellulase, hemicellulase, pectase and amylase is 0.1%, the extraction rate of main active ingredients reaches the maximum value, and the four enzymes are different in dosage and proportion, which is caused by the compatibility characteristics of the medicinal materials in the prescription in the oral liquid for clearing plague and detoxicating, and the cell wall structures of the medicinal materials in different rhizomes and fruits are different, so that the comprehensive research result shows that the following table 6 shows that the four different enzymes are selected in the research to have the proportion of 1:1:1:3.
TABLE 6 influence of enzyme dosage on baicalin content
Enzyme ratio | 1:0.5:1:5 | 1:1:2:2 | 1:1:2.5:1 | 1:1:1:3 |
Baicalin (mg/mL) | 6.03 | 5.98 | 6.31 | 6.98 |
3.2.2 enzyme reaction time
The enzyme reaction was examined by designing different time gradients and detecting baicalin content by HPLC method, and the results are shown in table 7 below, which shows that when the extraction time is equal to or higher than 30min, the enzyme reaction reached the maximum, and the main active ingredient had reached the maximum, so the experiment was selected to be the optimal time for the enzyme reaction for 30 min.
TABLE 7 influence of extraction time on baicalin content
Extraction time (min) | 10 | 20 | 30 | 60 |
Baicalin (mg/mL) | 5.56 | 6.55 | 6.98 | 7.01 |
3.2.3 optimal temperature for enzymatic reaction
The baicalin yield was examined by designing different enzyme reaction temperature gradients and detecting the baicalin content by HPLC method, as shown in table 8 below, when the enzymolysis temperature was 45 ℃, the main active ingredient reached the maximum value, and the temperature was lower or higher than this value, which affects the enzyme reaction efficiency, which was related to the optimum reaction temperatures of the four enzymes, thereby determining that 45 ℃ is the optimum temperature for the enzyme reaction.
TABLE 8 influence of enzyme reaction temperature on baicalin content
Enzyme reaction temperature (. Degree. C.) | 20 | 30 | 40 | 45 |
Baicalin (mg/mL) | 4.43 | 5.98 | 6.63 | 6.98 |
3.2.4 optimal pH for enzymatic reactions
The first yield of baicalin is examined by designing different pH gradients, and the baicalin content is detected by adopting an HPLC method, the test results are shown in table 9, when the pH reaches 5.0, the extraction rate of baicalin components reaches the maximum value, and 5.0 is selected as the optimal pH for enzymolysis due to the specificity of enzyme reaction to the pH.
TABLE 9 influence of pH on baicalin content
pH | 4 | 4.5 | 5 | 5.5 |
Baicalin (mg/mL) | 6.49 | 6.75 | 6.98 | 6.72 |
The research results of the test example show that: the optimal technical parameters of the biological enzymolysis wall-breaking extraction process are as follows: the optimal acting dosage of the compound enzyme is 0.1%, the proportion of cellulase, hemicellulase, pectase and amylase is 1:1:1:3, the pH is 5.0, the reaction time is 30min, and the reaction temperature is 45 ℃.
In summary, the optimal proportion relation of the invention is obtained by sieving the composition components, the crushing granularity, the heating time, the extraction temperature, the water addition amount, the enzyme dosage, the enzyme reaction time, the enzyme reaction optimal temperature and the enzyme reaction optimal pH value, so that the dissolution rate of the active ingredients is greatly improved, and the quality of the product is improved.
While specific embodiments of the invention have been described in detail, it should not be construed as limiting the scope of the patent. Various modifications and variations which may be made by those skilled in the art without the creative effort are within the scope of the patent described in the claims.
Claims (8)
1. The oral liquid for clearing away plague and toxic materials is characterized by being prepared from the following raw materials in parts by weight: 200-400 parts of coptis chinensis, 250-350 parts of radix isatidis, 180-220 parts of divaricate saposhnikovia root, 200-250 parts of baical skullcap root, 100-150 parts of honeysuckle, 130-170 parts of gypsum, 80-120 parts of Indian buead, 50-80 parts of honey-fried licorice root, 120-180 parts of figwort root, 5-10 parts of lotus seed and 5-10 parts of peppermint.
2. The oral liquid for clearing away plague and toxic materials according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 250-350 parts of coptis chinensis, 300-320 parts of radix isatidis, 200-210 parts of divaricate saposhnikovia root, 220-230 parts of baical skullcap root, 120-140 parts of honeysuckle, 130-150 parts of gypsum, 90-100 parts of poria cocos, 50-60 parts of honey-fried licorice root, 140-150 parts of figwort root, 5-8 parts of lotus seed and 5-8 parts of peppermint.
3. The oral liquid for clearing away plague and toxic materials according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 300 parts of coptis chinensis, 300 parts of isatis root, 200 parts of divaricate saposhnikovia root, 200 parts of baical skullcap root, 120 parts of honeysuckle, 150 parts of gypsum, 90 parts of Indian buead, 60 parts of honey-fried licorice root, 150 parts of figwort root, 8 parts of lotus seed and 5 parts of mint.
4. A method for preparing an oral liquid for clearing plague and detoxicating according to any one of claims 1-3, comprising the steps of:
(1) Mixing the components in proportion, crushing to 100-200 meshes, and then adding water with the weight 10-20 times of the weight of the mixture for heating and soaking;
(2) Filtering the mixture soaked in the step (1), heating by microwaves, heating to 60 ℃ firstly, then keeping for 10-30min, heating to 70 ℃ again, then keeping for 10-30min, and finally heating to 90 ℃ and keeping for 20-40min; separating the liquid and the solid;
(3) Soaking the solid in water, adjusting pH to 5.0, and adding cellulase, hemicellulase, pectase and amylase for enzymolysis; the weight of the added water is 5-8 times of that of the solid, the mass ratio of the cellulase to the hemicellulase to the pectase to the amylase is 1-2:1:1:3-5, the enzymolysis time is 30-40min, and the enzymolysis temperature is 35-45 DEG C
(4) Heating to extract liquid medicine after enzymolysis, mixing the liquid medicine with the liquid separated in the step (2), decocting for 3-5 times, mixing the decoctions, centrifuging, and micro-filtering the supernatant.
5. The method for preparing oral liquid according to claim 4, wherein the heating temperature in the step (1) is 35-45 ℃ and the soaking time is 60-120min.
6. The method for preparing oral liquid according to claim 4, wherein the final temperature of the heating in the step (4) is 75-85 ℃, and the extraction time is 45-55min.
7. The method for preparing the oral liquid for clearing away heat and toxic materials according to claim 4, wherein the step of microfiltration in the step (4) specifically comprises: and (3) circularly micro-filtering the supernatant by using an inorganic ceramic membrane micro-filter in a cross-flow mode, adding distilled water with the amount of 2 times of that of the medicinal materials, continuing micro-filtering, and concentrating the micro-filtered liquid.
8. The method for preparing the oral liquid for clearing away heat and toxic materials according to claim 7, wherein the parameters of the microfiltration comprise: the aperture of the inorganic ceramic membrane is 0.2 mu m, the flow rate of the feed is 30-50m/s, and the concentration multiple is 3-5 times.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211053465.4A CN115607603B (en) | 2022-08-31 | 2022-08-31 | Oral liquid for clearing away plague and toxic materials and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211053465.4A CN115607603B (en) | 2022-08-31 | 2022-08-31 | Oral liquid for clearing away plague and toxic materials and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN115607603A CN115607603A (en) | 2023-01-17 |
CN115607603B true CN115607603B (en) | 2023-07-04 |
Family
ID=84856460
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202211053465.4A Active CN115607603B (en) | 2022-08-31 | 2022-08-31 | Oral liquid for clearing away plague and toxic materials and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN115607603B (en) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106110119A (en) * | 2016-07-18 | 2016-11-16 | 安徽奥力欣生物科技有限公司 | A kind of antipyretic and detoxicated oral liquid for animals and preparation method thereof |
CN106177586A (en) * | 2016-08-31 | 2016-12-07 | 河南白云牧港生物科技有限公司 | A kind of antipyretic and detoxicated Chinese medicine composition for animals and preparation method thereof |
-
2022
- 2022-08-31 CN CN202211053465.4A patent/CN115607603B/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN115607603A (en) | 2023-01-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107595893A (en) | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof | |
CN112156156B (en) | Veterinary traditional Chinese medicine antiviral oral liquid and preparation method thereof | |
CN102961414A (en) | Chinese herbal medicine raw stock and application thereof | |
CN108524685B (en) | Composition for treating children herpangina and application thereof | |
CN104982597B (en) | A kind of multi-functional composite instant tea and its preparation method and application | |
CN112535239B (en) | Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof | |
CN100378089C (en) | Process for extracting total flavone from stem and leaf of scutellaria | |
CN105815703A (en) | Method for processing trichosanthes peel, cortex albiziae and hawthorn fruit healthcare powder | |
CN115607603B (en) | Oral liquid for clearing away plague and toxic materials and preparation method thereof | |
CN115844937B (en) | Preparation method and application of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects | |
CN112515014A (en) | Kudzuvine root polysaccharide sweet zong black tea and preparation method thereof | |
CN105638998A (en) | Composite tea with radix millettiae speciosae and preparation method thereof | |
CN113975357B (en) | Traditional Chinese medicine composition for clearing lung, eliminating phlegm, eliminating carbuncle, expelling pus, clearing heat and promoting diuresis and application thereof | |
CN109846932A (en) | A kind of extraction purification and technology of preparing of mulberries slag blood-sugar decreasing active | |
CN113694152B (en) | Method for obtaining coix seed extracting solution by high-stability enzymolysis method | |
CN105748628A (en) | Traditional Chinese medicine soluble powder for treating piglet diarrhea | |
CN114507297A (en) | Preparation and application of inonotus obliquus polysaccharide extract | |
CN113713004A (en) | Radix isatidis granules and preparation method thereof | |
CN111887367A (en) | Loquat flower beverage and preparation method thereof | |
CN108440477B (en) | A method of preparing Quercetin from tungoiltree juvenile leaf | |
CN103054916A (en) | Application of freeze-dried purslane in preparation of medicine for treating eczema | |
CN104161817B (en) | A kind of compound traditional Chinese medicine composite for preventing and treating chicken bacterial disease and preparation method thereof | |
CN108740478B (en) | Herbal extract feed additive for resisting heat stress of broiler chicken and preparation method thereof | |
CN112353880B (en) | Preparation method of children cold-relieving traditional Chinese medicine | |
CN110637934A (en) | Tea seed meal polysaccharide compound and application thereof in feed and treatment of porcine reproductive and respiratory syndrome |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |