CN115590894A - 一种海藻全活性物质的制备方法 - Google Patents
一种海藻全活性物质的制备方法 Download PDFInfo
- Publication number
- CN115590894A CN115590894A CN202211302065.2A CN202211302065A CN115590894A CN 115590894 A CN115590894 A CN 115590894A CN 202211302065 A CN202211302065 A CN 202211302065A CN 115590894 A CN115590894 A CN 115590894A
- Authority
- CN
- China
- Prior art keywords
- seaweed
- active substance
- residue
- drying
- dietary fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001474374 Blennius Species 0.000 title claims abstract description 60
- 239000013543 active substance Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 24
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 235000013325 dietary fiber Nutrition 0.000 claims description 8
- 238000009210 therapy by ultrasound Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 241000195628 Chlorophyta Species 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 241000199919 Phaeophyceae Species 0.000 claims description 3
- 108010059820 Polygalacturonase Proteins 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 241000206572 Rhodophyta Species 0.000 claims description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 235000019419 proteases Nutrition 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 239000012675 alcoholic extract Substances 0.000 claims 1
- 239000000469 ethanolic extract Substances 0.000 claims 1
- 238000010298 pulverizing process Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 239000002699 waste material Substances 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 7
- 210000002421 cell wall Anatomy 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 208000036071 Rhinorrhea Diseases 0.000 description 1
- 206010039101 Rhinorrhoea Diseases 0.000 description 1
- 206010000059 abdominal discomfort Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 208000014001 urinary system disease Diseases 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/03—Phaeophycota or phaeophyta (brown algae), e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/04—Rhodophycota or rhodophyta (red algae), e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/05—Chlorophycota or chlorophyta (green algae), e.g. Chlorella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Alternative & Traditional Medicine (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
本发明公开了一种海藻全活性物质的制备方法，所述海藻全活性物质利用超声同时酶解的方法制备。本发明提供了一种海藻活性物质的零浪费制备方法，该制备方法可以提高产率、减少污染、降低生产成本，并具有一定普适性，有着广阔的应用前景。
Description
技术领域
本发明属于海藻全活性物质超声同时酶解生产工艺，涉及一种海藻活性物质的零浪费制备方法，该活性物质具有很高的营养价值及药用价值，制备的海藻活性物质具备较高的生物活性，促进植物根的生长、抗菌，提高人体免疫力、抗肿瘤、增强体力等。
背景技术
海藻是指生长在潮间带及亚潮间带肉眼可见的大型藻类，通常包括绿藻、褐藻及红藻三大类。在古代中国及日本就有利用海藻做为食物的证据，古医典包括《本草纲目》、《本草经集注》、《海药本草》及《本草拾遗》等都有用海藻治疗各种疾病的纪载。许多种海藻依其化学成分不同而有不同的药理作用及临床用途。可以用于退烧、治咳，以及治疗气喘、痔疮、流鼻涕、肠胃不适及泌尿疾病等。还可以加强身体抗癌、抗肿瘤的能力，且可有效改善糖尿病症状及纾解紧张压力。
海藻中的活性成分丰富，包括多糖、多酚等丰富的活性物质，但目前工业上对海藻活性物质的提取方法单一，运用大量的有机试剂等不仅容易导致产品降解严重、黏度降低，还会对环境造成严重的污染。近年来，又逐渐发展了以酶解或超声处理代替有机体系浸泡的酶解法和超声法。酶解法是在一定条件下用酶分解海藻细胞壁，促进海藻活性物质溶出，从而提高海藻活性物质产率的方法。酶解法具有专一性强、条件温和、工艺简单等优点。但是，单一酶存在酶解效果不佳、生产成本高、工艺周期长且产品纯度不高等问题，超声法利用超声波使海藻细胞壁在瞬间破裂，并通过振动作用和热效应加速细胞内有效物质的释放、扩散和溶解，有助于海藻活性物质溶出，使产率显著提高，但是超声法耗时较长，目前超声和酶解也多为单独使用，并未得到有效结合，工艺方法尚未成熟。
超声-复合分步酶解法能有效破坏海藻细胞壁的结构，不仅能够提高海藻活性物质的产率、缩短生产周期、减少环境污染，还通过减少酸和碱的用量，有效降低生产成本。同是该方法适用于所有大型海藻，对各种海藻多糖的提取有参考价值，对大型海藻类生物质资源的高附加值利用有借鉴意义。本发明中通过超声-复合分步酶解法制备具有抗氧化活性的海藻全活性物质均未见其它研究报道或专利。
发明内容
本发明的目的是提供一种海藻全活性物质的绿色制备方法。制备工艺路线简单合理、绿色高效，是一种制备海藻全活性物质的有效方法。为实现上述目的，本发明采用如下技术方案：
一种海藻全活性物质的制备方法，包含有如下步骤：
（1）将海藻原料洗净后烘干，之后用粉碎机粉碎成海藻颗粒（或粗粉）； （2）往海藻粉中加入10-20倍重量的体积分数55%-75%乙醇溶液为提取溶剂在65-80℃回流提取2-3h，用50-500目尼龙布过滤获得海藻醇提液和海藻渣1；
（3）往海藻渣1中加入10-50倍重量的蒸馏水，加入海藻渣1重量0.1-0.5%纤维素酶+0.1-0.5%果胶酶，调整pH=3-6，酶解和超声处理2-3h后，加入海藻渣1重量0.1-0.5%复合蛋白酶+0.2-0.6%木瓜蛋白酶，调整pH=6-9, 酶解和超声处理2-3h后，用90-130℃灭酶10-20min后，过滤，得到海藻活性物质提取液和海藻渣2回收烘干。
（4）往海藻渣2中加入其质量1-5%的无水碳酸钠以及20-50倍重量的蒸馏水，55-85℃超声提取2-3h，离心过滤得滤渣放入烘箱进行烘干得海藻不可溶性膳食纤维，滤液浓缩后，热风干燥、粉碎获得可溶性膳食纤维。
进一步的，步骤（1）中，所述海藻可以是绿藻、红藻、褐藻等，应用范围广。
进一步的，步骤（2）（3）（4）中，所述的加工工艺中所用试剂都是无毒安全试剂。
进一步的，步骤（3）中，超声-复合分步酶解法能有效破坏海藻细胞壁的结构，不仅能够提高海藻活性物质的产率、缩短生产周期、减少环境污染，还通过减少酸和碱的用量，有效降低生产成本。
进一步的，步骤（2）（3）（4）中，所述的提取过程将海藻的所有活性物质进行提取，做到零浪费，操作简便，同时可以连续地大规模进行生产，适合大规模化工业生产。
本发明的优点是：
（1）本发明的提取方法合理有效，利用超声-复合分步酶解的零浪费制备方法制备，具有无副反应、条件温和、环境友好、节约能耗等优点；
（2）本发明采购所有海藻进行提取，极大的利用了海藻资源；
（3）本发明所有超声-复合分步酶解法能有效破坏海藻细胞壁的结构，不仅能够提高海藻活性物质的产率、缩短生产周期、减少环境污染，还通过减少酸和碱的用量，有效降低生产成本。
具体实施方式
下面通过实施例对本发明做进一步详细描述， 但本发明不仅仅局限于以下实施例。
实施例1
一种海藻全活性物质的绿色制备方法，包括以下步骤：
（1）将海带原料洗净后烘干，之后用粉碎机粉碎成海带颗粒（或粗粉）； （2）往海带粉中加入20倍重量的体积分数55%乙醇溶液为提取溶剂在65℃回流提取2h，用300目尼龙布过滤获得海带醇提液和海带渣1；
（3）往海带渣1中加入40倍重量的蒸馏水，加入海带渣1重量0.1%纤维素酶+0.1%果胶酶，调整pH=3，酶解和超声处理2h后，加入海带渣1重量0.1%复合蛋白酶+0.2%木瓜蛋白酶，调整pH=6, 酶解和超声处理2h后，用100℃灭酶10min后，过滤，得到海带活性物质提取液和海带渣2回收烘干。
（4）往海带渣2中加入其重量1%的无水碳酸钠以及30倍重量的蒸馏水，55℃超声提取2h。离心过滤得滤渣放入烘箱进行烘干得海带不可溶性膳食纤维，滤液浓缩后，热风干燥、粉碎获得可溶性膳食纤维。
（5）对海带全活性物质得率及其活性物质含量进行测定。结果如下：
1. 海带全活性物质各物质产量及得率
2.多糖测定
多糖含量的具体测定方法为吸取0、0.2、0.4、0.6、0.8、1.0 mL的0.1mg/mL的标准葡萄糖溶液于试管中，蒸馏水补至1.0 mL，加入5%的苯酚溶液1.0 mL和5.0 mL的硫酸溶液，避光静置10 min，涡旋混匀，转入30 ℃水浴锅中反应20 min，吸取200 μL于96孔板中490nm处测吸光值。得出葡萄糖标准曲线为：y = 0.00061x + 0.1919，R² = 0.9983。
3. 蛋白测定
采用BCA试剂盒法测定海带活性物质中的蛋白含量。按照试剂盒的说明书配置取的蛋白标准溶液(20 mg/mL)，将标准溶液依次0、1、2、4、8、12、16、20μL的加到96孔板中，加稀释液补足至20μL，其蛋白浓度依次为0、25、50、100、200、300、400、500μg/mL，加20μL的待测样品到96孔板中。37℃孵育20-30min，在酶标仪562nm处测定吸光度，根据标准曲线计算出待测样品的蛋白浓度。蛋白标准曲线为：y = 0.0009x + 0.0827，R² = 0.9971。
本发明能够提高海藻活性物质的产率，缩短生产周期。
以上所述仅为本发明的较佳实施例，凡依本发明申请专利范围所做的均等变化与修饰，皆应属本发明的涵盖范围。
Claims (4)
1.一种海藻全活性物质的制备方法，其特征在于，包含有如下步骤：
（1）将海藻原料洗净后烘干、粉碎，加入10-20倍重量的体积分数55%-75%乙醇溶液在65-80℃回流提取2-3h，用50-500目尼龙布过滤获得海藻醇提液和海藻渣1；
（2）往海藻渣1中加入10-50倍重量的蒸馏水，加入0.1-0.5wt%纤维素酶和0.1-0.5wt%果胶酶 ，调整pH=3-6，酶解和超声处理2-3h后，加0.1-0.5wt%复合蛋白酶和0.2-0.6wt%木瓜蛋白酶，调整pH=6-9，酶解和超声处理2-3h后，用90-130℃灭酶10-20min后，过滤，得到海藻活性物质提取液和海藻渣2回收烘干；
（3）往海藻渣2中加入其质量1-5%的无水碳酸钠以及20-50倍重量的蒸馏水，55-85℃超声提取2-3h，离心过滤得滤渣放入烘箱进行烘干得海藻不可溶性膳食纤维，滤液浓缩后，热风干燥、粉碎获得可溶性膳食纤维。
2. 根据权利要求 1 所述的制备方法，其特征在于，所述海藻包括绿藻、红藻、褐藻。
3. 根据权利要求 1 所述的制备方法，其特征在于，所述海藻为海带。
4. 根据权利要求 1 所述的制备方法，其特征在于，所述海藻全活性物质包括海藻醇提液、海藻活性物质提取液、不可溶性膳食纤维和可溶性膳食纤维。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211302065.2A CN115590894A (zh) | 2022-10-24 | 2022-10-24 | 一种海藻全活性物质的制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211302065.2A CN115590894A (zh) | 2022-10-24 | 2022-10-24 | 一种海藻全活性物质的制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115590894A true CN115590894A (zh) | 2023-01-13 |
Family
ID=84848380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211302065.2A Pending CN115590894A (zh) | 2022-10-24 | 2022-10-24 | 一种海藻全活性物质的制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115590894A (zh) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608512A (zh) * | 2004-11-22 | 2005-04-27 | 青岛明月海藻集团有限公司 | 海带精及其制备方法 |
| CN104177136A (zh) * | 2013-05-21 | 2014-12-03 | 徐波勇 | 一种从海藻中提取肥料用活性物质的方法 |
| CN104256252A (zh) * | 2014-09-15 | 2015-01-07 | 福建农林大学 | 一种分步提取与生物转化偶联制备海带降血压制品的方法 |
| CN106478554A (zh) * | 2016-10-17 | 2017-03-08 | 青岛博恩高科生物技术有限公司 | 一种从海藻中综合提取岩藻黄质和海藻多糖的方法 |
| CN106942739A (zh) * | 2017-03-15 | 2017-07-14 | 福建农林大学 | 一种海藻全活性物质的制备方法 |
-
2022
- 2022-10-24 CN CN202211302065.2A patent/CN115590894A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608512A (zh) * | 2004-11-22 | 2005-04-27 | 青岛明月海藻集团有限公司 | 海带精及其制备方法 |
| CN104177136A (zh) * | 2013-05-21 | 2014-12-03 | 徐波勇 | 一种从海藻中提取肥料用活性物质的方法 |
| CN104256252A (zh) * | 2014-09-15 | 2015-01-07 | 福建农林大学 | 一种分步提取与生物转化偶联制备海带降血压制品的方法 |
| CN106478554A (zh) * | 2016-10-17 | 2017-03-08 | 青岛博恩高科生物技术有限公司 | 一种从海藻中综合提取岩藻黄质和海藻多糖的方法 |
| CN106942739A (zh) * | 2017-03-15 | 2017-07-14 | 福建农林大学 | 一种海藻全活性物质的制备方法 |
Non-Patent Citations (4)
| Title |
|---|
| 樊文乐,武文洁: "褐藻糖胶的提取方法", 中国食品添加剂, no. 05, pages 103 - 106 * |
| 武文洁;樊文乐;衣守志;: "海带综合利用研究", 食品科技, no. 01, pages 49 - 52 * |
| 董学前;张艳敏;张永刚;赵林;王伟;刘建军;: "复合酶法综合提取海带中褐藻糖胶与海藻酸的研究", 中国食品添加剂, no. 09, pages 171 - 176 * |
| 陈玉青;严新;陈明军;林娈;杨成凤;李秋哲;刘斌;赵超: "淡黑巨藻醇提取物降血糖活性及其对小鼠肠道菌群的影响", 生物技术通报, vol. 33, no. 12, pages 162 - 169 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107858393B (zh) | 一种从核桃粕中提取蛋白多肽的方法 | |
| CN104480161A (zh) | 一种超微粉碎辅助酶法制备小麦麸皮低聚糖的方法 | |
| CN101897431B (zh) | 一种同时制备酵母抽提物和β-1，3-葡聚糖的方法 | |
| CN110272932B (zh) | 一种灵芝孢子粉多糖肽的制备方法 | |
| CN105399855A (zh) | 一种球磨制备燕麦β-葡聚糖的方法 | |
| CN101283760A (zh) | 从桃渣中提取制备膳食纤维的方法 | |
| CN103834713B (zh) | 一种剑麻皂素的提取方法 | |
| CN103266154A (zh) | 制备高活性茶皂甙的生物转化方法 | |
| CN112869164B (zh) | 一种高含β-烟酰胺单核苷酸(NMN)西蓝花提取物的制备方法 | |
| CN107312807A (zh) | 一种海带来源的褐藻功能寡糖的酶解制备方法 | |
| CN115260334B (zh) | 一种桑叶多糖的复合提取工艺 | |
| CN101492706A (zh) | 一种提高蛹虫草液体发酵虫草菌素产量的方法 | |
| CN108048495B (zh) | 一种白藜芦醇的生物提取方法 | |
| CN106046189A (zh) | 南瓜多糖的提取与纯化方法 | |
| CN103864954B (zh) | 一种花生粕多糖的提取方法 | |
| CN113584102A (zh) | 具有抗氧化活性的微拟球藻多糖的制备方法 | |
| CN1884571B (zh) | 从石蒜属植物中提取多糖的工艺方法 | |
| CN110283860B (zh) | 超声波辅助复合酶解提取的细基江蓠多糖及其提取方法 | |
| CN115590894A (zh) | 一种海藻全活性物质的制备方法 | |
| CN113999327B (zh) | 一种具有显著抗光老化活性的羊栖菜多糖及其应用 | |
| CN101367883A (zh) | 一种从发酵液中提取灵芝多糖的方法 | |
| CN113694152B (zh) | 一种高稳定性酶解法获取薏苡仁提取液的方法 | |
| CN115852723A (zh) | 一种利用玉米秸秆提取高纯度木糖液和纤维素的方法 | |
| CN116144506A (zh) | 土曲霉dk3-18及其在提取柿叶总黄酮中的应用 | |
| CN111485012A (zh) | 一种甘草发酵制备单葡萄糖醛酸甘草次酸的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20230113 |