CN115363054B - Thrips insecticide prepared from amur ampelopsis extract - Google Patents

Thrips insecticide prepared from amur ampelopsis extract Download PDF

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CN115363054B
CN115363054B CN202211207392.XA CN202211207392A CN115363054B CN 115363054 B CN115363054 B CN 115363054B CN 202211207392 A CN202211207392 A CN 202211207392A CN 115363054 B CN115363054 B CN 115363054B
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thrips
ampelopsis
extract
ampelopsis grossedentata
insecticide
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CN115363054A (en
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常大勇
孙明明
廖俊彦
贾朝佩
丁玮琳
王琳
宋兆福
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Hainan Changhai Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Insects & Arthropods (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a thrips insecticide prepared from a ampelopsis grossedentata extract, which belongs to the field of agricultural application of plant extracts, and is characterized by comprising the following components in parts by weight: 1-10% of a ampelopsis grossedentata extract, 3-15% of a dispersing agent, 1-10% of an antioxidant and 50-90% of sterile water. The invention creatively uses the extract of the amur ampelopsis grossedentata for preparing the thrips insecticide, creates a new application way of the amur ampelopsis grossedentata, and can effectively reduce the drug resistance of thrips. The prepared pesticide has obvious effect of controlling thrips, strong pertinence, environmental protection, no toxicity and no residue. Provides a green and environment-friendly biological source preparation capable of replacing pesticides for preventing and controlling thrips.

Description

Thrips insecticide prepared from amur ampelopsis extract
Technical Field
The invention belongs to the field of development and application of plant active ingredients and agricultural pesticides, and particularly relates to a thrips pesticide prepared from a ampelopsis grossedentata extract.
Background
Thrips thysanoptera insects are high-rise insect pest species in fields in recent years, and the number of thrips inhabiting on crop vegetables in China is up to 44. Thrips are usually ignored by people in the early period of hazard because individuals are tiny, have more concealed occurrence, rapid propagation, strong drug resistance, are difficult to find and are easy to disaster, and often pay attention to people after serious hazard. The normal growth and development of vegetable plants are seriously affected, and the improvement of the edible quality of vegetables is also affected, so that the market value of vegetables is greatly reduced, and huge economic loss is caused to agricultural production directly or indirectly every year.
At present, the method for preventing and controlling thrips is widely applied and is chemical agent prevention and control, and few attempts are made on biological pesticides and agricultural measures prevention and control methods, so that the application trend is not formed. However, the long-term application of chemical pesticide control is difficult to achieve the aim of high-efficiency control, and the pesticide residue is out of standard, the ecological imbalance and the drug resistance of thrips are obviously enhanced. Therefore, it is important to find a safe and efficient way capable of continuously preventing and controlling thrips.
The amur ampelopsis Ampelopsis glandulosa var, alterophylla (thunderberg) Momiyama, also called amur ampelopsis and wild grape, is used for promoting blood circulation, removing blood stasis, diminishing inflammation, removing toxicity, promoting tissue regeneration, and the like, and is one of resource plants used as ampelopsis root medicines in the Hubei Massa Medicata Fermentata area.
The Ampelopsis plant of Vitaceae has more than 30 kinds worldwide, 17 kinds in our country, and is widely distributed in southwest, south China and northeast China. Since the 70 th century of the 20 th century, scholars at home and abroad have made a great deal of researches on various medicinal plants of Ampelopsis in terms of plant taxonomies, raw pharmacology, chemical compositions, clinical applications and pharmacological actions. However, little research is carried out on the isoleaf ampelopsis, and the research on insect resistance of the isoleaf ampelopsis is not reported so far. The beneficial substances in the isotophus ampelopsis are extracted to prepare the biological preparation with killing effect on thrips, and the method has important significance in replacing chemical pesticides, reducing environmental pollution, reducing production cost, increasing agricultural product value, improving peasant income and assisting village vibration development strategy.
Through inquiry, the invention patent 'a traditional Chinese medicine feed formula for preventing and treating poultry parasitic diseases' (patent application number: 201510949065.5) discloses a traditional Chinese medicine feed formula for preventing and treating poultry coccidiosis by adding ampelopsis grossedentata roots. Wherein the Ampelopsis grossedentata root is not of the same species as the Ampelopsis grossedentata of the present invention. The extraction mode and the process steps of the ampelopsis grossedentata root have obvious differences from the invention. The application range of the invention is the prevention and treatment of the poultry parasitic disease, and the application range of the invention is obviously different from that of the invention in the prevention and treatment of thrips.
The thrips insecticide prepared from the ampelopsis grossedentata extract has the beneficial effect of effectively killing thrips, can effectively replace chemical pesticides, and reduces the drug resistance of thrips. Is an application innovation of the amur ampelopsis extract, and provides a new environment-friendly preparation for preventing and controlling thrips insect pests.
Disclosure of Invention
The invention aims at designing a thrips insecticide prepared from a ampelopsis grossedentata extract, and is characterized in that the ampelopsis grossedentata is a traditional Chinese herbal medicine, the application safety is high, and the extraction process is simple. The prepared pesticide has high-efficiency killing effect on thrips, is safe and environment-friendly to agricultural products, can effectively replace chemical pesticides, and reduces the drug resistance of thrips. The preparation is not only an innovative application of the ampelopsis grossedentata extract, but also provides a new environment-friendly preparation for biological control of thrips insect pests.
The specific technical scheme comprises the following steps:
1): crushing the dried isoleaf ampelopsis root, sieving with a 20-40 mesh sieve, removing impurities such as crude fibers and the like, and obtaining isoleaf ampelopsis powder;
2): weighing 100g of isoleaf ampelopsis powder, adding 1000mL of 95% ethanol, and fully mixing to obtain a mixed solution;
3): ultrasonic extracting the mixed solution by using an ultrasonic oscillator with the power of 300-450W, wherein the ultrasonic frequency is 15-20 kHz, the temperature is 25-65 ℃ and the time is 20-60 min, and vacuum filtering is carried out by using a filter membrane with the aperture of 0.22 mu m to obtain filtrate 1 and filter residue 1;
4): adding 1000mL of 95% ethanol into the filter residue 1, uniformly mixing, and performing the same treatment as in the step 3) to obtain a filtrate 2 and a filter residue 2;
5): mixing the filtrates 1 and 2, concentrating under reduced pressure at 60 ℃ and-0.1 MPa for 20-40 min to obtain a Ampelopsis grossedentata extract;
6): 1 to 10 parts of the ampelopsis grossedentata extract, 3 to 15 parts of dispersing agent, 1 to 10 parts of antioxidant and 50 to 90 parts of sterile water are uniformly mixed to prepare the thrips insecticide.
The dispersing agent in the step 6) is polyethylene glycol 200 or 400, the molecular weight is 190-420, and the antioxidant is vitamin C, astaxanthin or beta-carotene.
The thrips insecticide prepared from the isoleaf ampelopsis grossedentata extract is characterized in that on the basis of fully verifying the effectiveness of the isoleaf ampelopsis grossedentata extract on thrips control, in order to enhance the control effect and increase the action persistence, multi-factor and multi-gradient comparison tests are carried out, and the preparation formula with the optimal effect is finally determined through continuous optimization and verification, so that the insecticide has remarkable innovation and creativity.
Compared with the existing thrips insecticide, the invention has the relevant advantages of plant extracts: biological sources, environmental protection, no residue and no pollution, and the direct application to leaf surfaces and fruit surfaces can not cause adverse effects on the quality of crops and agricultural products; the material is derived from plants and is not limited by chemical raw materials; the preparation process is simple, and three wastes are not generated in the preparation process; the extraction medium is recycled, and the production cost is low; the preparation has wide effect and good prevention effect on various thrips; the action mechanism is diversified, and the pests are not easy to generate drug resistance.
The invention has the innovation point that the isoleaf ampelopsis grossedentata extract is innovatively applied to the field of disinsection, the isoleaf ampelopsis grossedentata extract is used as the main active ingredient to prepare the thrips insecticide, and the effect of the thrips insecticide prepared from the isoleaf ampelopsis grossedentata extract on killing thrips is verified, so that the invention is an innovation of the application range of the isoleaf ampelopsis grossedentata extract and an innovation of a thrips pest control method.
The invention has the beneficial effects that the isotophus mulberry mistletoe extract is applied to the field of disinsection, and the prepared thrips insecticide has good control effect on various thrips pests. Provides a new application way for the amur ampelopsis and a new biological source preparation for effectively preventing and controlling thrips insect pests.
Description of the drawings:
fig. 1 shows test data of thrips insecticide spraying cotton pests, namely scallion thrips, and the insecticide effect is calculated according to the mortality rate, and the insecticide effect is verified within 5 days after spraying.
Figure 2 shows test data of thrips insecticide spraying tobacco insect rice thrips prepared by the invention, the insecticidal effect is calculated according to the mortality rate, and the insecticidal effect is verified within 5 days of spraying.
Figure 3 shows the test data of the large Jiang Haichong yellow thrips sprayed by the thrips insecticide prepared by the invention, the insecticidal effect is calculated according to the mortality rate, and the insecticidal effect is verified within 5 days after spraying.
Detailed Description
The principles and features of the present invention are described below in connection with examples, which are set forth only to illustrate the present invention and not to limit the scope of the invention.
Example 1
The invention provides a thrips insecticide prepared from a ampelopsis grossedentata extract, which is prepared by the following steps:
1): pulverizing dried radix Ampelopsis Brevipedunculatae, sieving with 20 mesh sieve, and removing impurities such as crude fiber to obtain powder;
2): weighing 100g of isoleaf ampelopsis powder, adding 1000mL of 95% ethanol, and fully mixing to obtain a mixed solution;
3): ultrasonic extracting the mixed solution by using an ultrasonic oscillator with the power of 450W, wherein the ultrasonic frequency is 20kHz, the temperature is 65 ℃ and the time is 60min, and vacuum filtering is carried out by using a filter membrane with the aperture of 0.22 mu m to obtain filtrate 1 and filter residue 1;
4): adding 1000mL of 95% ethanol into the filter residue 1, uniformly mixing, and performing the same treatment as in the step 3) to obtain a filtrate 2 and a filter residue 2;
5): mixing the filtrates 1 and 2, concentrating under reduced pressure at 60deg.C and-0.1 MPa for 40min to obtain Ampelopsis grossedentata extract;
6): mixing the extract of Ampelopsis grossedentata 5 parts, dispersant 6 parts, antioxidant 5 parts, and sterile water 84 parts to obtain the thrips insecticide.
Example 2
The invention provides a thrips insecticide prepared from a ampelopsis grossedentata extract, which is prepared by the following steps:
1): pulverizing dried radix Ampelopsis Brevipedunculatae, sieving with 40 mesh sieve, and removing impurities such as crude fiber to obtain powder;
2): weighing 100g of isoleaf ampelopsis powder, adding 1000mL of 95% ethanol, and fully mixing to obtain a mixed solution;
3): ultrasonic extracting the mixed solution by using an ultrasonic oscillator with the power of 450W, wherein the ultrasonic frequency is 15kHz, the temperature is 45 ℃ and the time is 30min, and vacuum filtering is carried out by using a filter membrane with the aperture of 0.22 mu m to obtain filtrate 1 and filter residue 1;
4): adding 1000mL of 95% ethanol into the filter residue 1, uniformly mixing, and performing the same treatment as in the step 3) to obtain a filtrate 2 and a filter residue 2;
5): mixing the filtrates 1 and 2, concentrating under reduced pressure at 60deg.C and-0.1 MPa for 30min to obtain Ampelopsis grossedentata extract;
6): 3 parts of a ampelopsis grossedentata extract, 8 parts of a dispersing agent, 5 parts of an antioxidant and 84 parts of sterile water are uniformly mixed to prepare the thrips insecticide.
Comparative example
Referring to the invention patent of a traditional Chinese medicine feed formula (patent application number: 201510949065.5) for preventing and treating poultry parasitic diseases, a ampelopsis grossedentata root paste extract is prepared, and alternative ampelopsis grossedentata extracts are uniformly mixed with other components in the embodiment 1 according to the proportion to prepare a comparative proportion.
Test 1
Thrips insect pests occur in the two cotton fields of red star of a thirteenth army of Xinjiang construction in the middle 6 th year of 2022, and the insect bodies are identified as the spring onion thrips. 90 adult spring onion thrips are selected for insecticidal test, wherein 30 spring onion thrips are placed in the net cage of the embodiment 1, and the embodiment 1 group insecticide diluted 1000 times is sprayed; 30 comparative example group insecticide which is placed in a comparative example net cage and is sprayed and diluted 1000 times; 30 net cages were placed in a negative control group, and an equal amount of sterile water was sprayed. And respectively observing and counting the insect bodies 0-5 days after spraying, and calculating the insecticidal effect according to the mortality rate.
The statistics are shown in Table 1.
Table 1 insecticide spray cotton pest test data for chives thrips
The analysis statistical data shows that the death rate of the spring onion thrips reaches 85.71% after the insecticide of the example 1 is sprayed for 5 days in the example 1, and the death rate of the spring onion thrips is only 10.71% after the insecticide of the comparative example is sprayed for 5 days, so that the insecticide has a higher killing effect on the spring onion thrips.
Test 2
The insecticidal test is carried out on 180 adult rice thrips collected from a garden in the next 5 th month of 2022, namely, temporary setting 264,264, temple of county, mountain jujube village flue-cured tobacco plantation, and the average is divided into 3 groups: 60 of the insecticide compositions are placed in a net cage of the example 2, and the insecticide composition of the example 2 is sprayed and diluted 1000 times; placing 60 pesticides in a comparative example box, spraying and diluting the pesticides in a comparative example box by 1000 times; 60 negative control net cages were placed and equal amount of sterile water was sprayed. And respectively observing and counting the insect bodies 0-5 days after spraying, and calculating the insecticidal effect according to the mortality rate.
The statistics are shown in Table 2.
Table 2 insecticide spraying tobacco pest rice thrips test data
The analysis statistical data shows that the death rate of rice thrips reaches 78.95% after the insecticide of the example 2 is sprayed for 5 days in the group of the example 2, and the death rate of onion thrips is only 10.53% after the insecticide of the comparative example group is sprayed for 5 days, so that the insecticide has a higher killing effect on rice thrips.
Test 3
In the middle 7 th 2022, yellow thrips exploded in the planting field of the Dagay ginger, tiger valley, yi-water county, yi-city, are collected, adult Huang Jima of the yellow thrips is selected for insecticidal test, and the average of the yellow thrips is divided into 3 groups: the first group is 40 experimental groups, and the insecticide of the example 1 is sprayed and diluted 1000 times; the second group is 40 comparative examples, and the comparative example insecticide diluted 1000 times is sprayed; the third group was 40 negative control groups, and an equal amount of sterile water was sprayed. And respectively observing and counting the insect bodies 0-5 days after spraying, and calculating the insecticidal effect according to the mortality rate.
The statistics are shown in Table 3.
TABLE 3 data on yellow thrips test with insecticide spray up Jiang Haichong
The analysis of statistical data shows that the mortality rate of Huang Jima reaches 69.23% after the insecticide of example 1 is sprayed for 5 days in the group of example 1, and the mortality rate of Huang Jima is only 15.38% after the insecticide of comparative example group is sprayed for 5 days, which shows that the insecticide has a higher killing effect on the thrips.
The previous description of the disclosed examples is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these examples will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (5)

1. A thrips insecticide prepared from a ampelopsis grossedentata extract is characterized by comprising the following components in percentage by weight: 1-10% of a ampelopsis grossedentata extract, 3-15% of a dispersing agent, 1-10% of an antioxidant and 50-90% of sterile water;
the preparation method of the isoleaf ampelopsis grossedentata extract comprises the following steps:
(1) Crushing the dried isoleaf ampelopsis root, and sieving the crushed isoleaf ampelopsis root with a 20-40 mesh sieve to obtain isoleaf ampelopsis powder;
(2) Weighing 100g of isoleaf ampelopsis powder, adding 1000mL of 95% ethanol, and fully mixing to obtain a mixed solution;
(3) Ultrasonic extracting the mixed solution by using an ultrasonic oscillator with the power of 300-450W, wherein the ultrasonic frequency is 15-20 kHz, the temperature is 25-65 ℃ and the time is 20-60 min, and vacuum filtering is carried out by using a filter membrane with the aperture of 0.22 mu m to obtain a filtrate 1 and a filter residue 1;
(4) Adding 1000mL of 95% ethanol into the filter residue 1, uniformly mixing, and performing the same treatment as in the step (3) to obtain a filtrate 2 and a filter residue 2;
(5) And (3) mixing the filtrate 1 and the filtrate 2, and concentrating under reduced pressure at 60 ℃ and-0.1 MPa for 20-40 min to obtain the isoleaf ampelopsis grossedentata extract.
2. The thrips insecticide prepared from the amur ampelopsis grossedentata extract according to claim 1, wherein the dispersing agent is polyethylene glycol 200 or polyethylene glycol 400 and has a molecular weight of 190-420.
3. The thrips insecticide prepared from the extract of ampelopsis grossedentata of claim 1, wherein the antioxidant is one of vitamin C, astaxanthin and beta-carotene.
4. Use of a thrips insecticide prepared from an extract of Ampelopsis grossedentata according to any one of claims 1-3 for controlling thrips cucullata, thrips fistulosa, thrips oryzae, thrips frankliniella occidentalis, and thrips zingiberis Dahurica.
5. The application according to claim 4, wherein the composition is used for controlling watermelon, white gourd, balsam pear, eggplant, tomato, onion, garlic, cotton, rice, corn, tobacco, melon thrips on ginger, spring onion thrips, rice thrips, frankliniella occidentalis and yellow thrips.
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008137940A (en) * 2006-12-01 2008-06-19 Taiyo Corp Thrips controlling agent
CN105533233A (en) * 2015-12-18 2016-05-04 杜小卫 Traditional Chinese medicine feed formula for preventing and treating parasitic diseases of poultry
CN107637349A (en) * 2017-09-25 2018-01-30 广西壮族自治区农业科学院葡萄与葡萄酒研究所 A kind of method of prevention and control grape thrips
CN107821448A (en) * 2017-11-09 2018-03-23 成都艾比科生物科技有限公司 A kind of pharmaceutical composition for killing thrips
CN107889857A (en) * 2017-11-09 2018-04-10 成都艾比科生物科技有限公司 A kind of preparation method for killing thrips pharmaceutical composition
CN110250224A (en) * 2019-06-26 2019-09-20 贵阳学院 A kind of preparation for preventing and treating Frankliniella occidentalis on vegetables

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008137940A (en) * 2006-12-01 2008-06-19 Taiyo Corp Thrips controlling agent
CN105533233A (en) * 2015-12-18 2016-05-04 杜小卫 Traditional Chinese medicine feed formula for preventing and treating parasitic diseases of poultry
CN107637349A (en) * 2017-09-25 2018-01-30 广西壮族自治区农业科学院葡萄与葡萄酒研究所 A kind of method of prevention and control grape thrips
CN107821448A (en) * 2017-11-09 2018-03-23 成都艾比科生物科技有限公司 A kind of pharmaceutical composition for killing thrips
CN107889857A (en) * 2017-11-09 2018-04-10 成都艾比科生物科技有限公司 A kind of preparation method for killing thrips pharmaceutical composition
CN110250224A (en) * 2019-06-26 2019-09-20 贵阳学院 A kind of preparation for preventing and treating Frankliniella occidentalis on vegetables

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掌裂草葡萄根皮杀虫成分的初步研究;邓保炜;杜芳艳;刘媛;;光谱实验室(第05期);全文 *

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