LU504686B1 - Composition for preventing and treating asparagus stem blight and preparation method thereof - Google Patents
Composition for preventing and treating asparagus stem blight and preparation method thereof Download PDFInfo
- Publication number
- LU504686B1 LU504686B1 LU504686A LU504686A LU504686B1 LU 504686 B1 LU504686 B1 LU 504686B1 LU 504686 A LU504686 A LU 504686A LU 504686 A LU504686 A LU 504686A LU 504686 B1 LU504686 B1 LU 504686B1
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- Prior art keywords
- extract
- thunb
- houttuynia cordata
- gagn
- artemisiacapillaris
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- 235000005340 Asparagus officinalis Nutrition 0.000 title claims abstract description 72
- 239000000203 mixture Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 244000003416 Asparagus officinalis Species 0.000 title claims description 68
- 239000000284 extract Substances 0.000 claims abstract description 110
- 240000000691 Houttuynia cordata Species 0.000 claims abstract description 50
- 235000013719 Houttuynia cordata Nutrition 0.000 claims abstract description 50
- 241000033009 Merremia Species 0.000 claims abstract description 43
- 235000008658 Artemisia capillaris Nutrition 0.000 claims abstract description 40
- 241000092668 Artemisia capillaris Species 0.000 claims abstract description 40
- 239000000419 plant extract Substances 0.000 claims abstract description 23
- 239000004094 surface-active agent Substances 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 23
- 239000000706 filtrate Substances 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 14
- 238000010298 pulverizing process Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 108010059892 Cellulase Proteins 0.000 claims description 9
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 9
- 229940106157 cellulase Drugs 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000003085 diluting agent Substances 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 230000012010 growth Effects 0.000 abstract description 5
- 238000011282 treatment Methods 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 241000234427 Asparagus Species 0.000 abstract 4
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 28
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 21
- 230000000052 comparative effect Effects 0.000 description 20
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 239000003814 drug Substances 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 244000124853 Perilla frutescens Species 0.000 description 5
- 235000004348 Perilla frutescens Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 239000000447 pesticide residue Substances 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 239000011669 selenium Substances 0.000 description 3
- 229910052711 selenium Inorganic materials 0.000 description 3
- 235000011649 selenium Nutrition 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000013628 Lantana involucrata Nutrition 0.000 description 2
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 description 2
- 240000007673 Origanum vulgare Species 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- XLYLQUQHYUOPIW-UHFFFAOYSA-N 3-oxo-Tetradecanal Chemical compound CCCCCCCCCCCC(=O)CC=O XLYLQUQHYUOPIW-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000123643 Asparagaceae Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 241000207782 Convolvulaceae Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- QBDCOUHKEVYWLO-UHFFFAOYSA-N Decanoylacetaldehyde Natural products CCCCCCCCCC(=O)CC=O QBDCOUHKEVYWLO-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 241001645346 Phomopsis asparagi Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000758742 Saururaceae Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000036531 allelopathy Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229960003284 iron Drugs 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 229940091258 selenium supplement Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Dentistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a composition for preventing and treating asparagus stem blight and a preparation method thereof, and belongs to the technical field of agricultural biological control. The composition of the invention consists of 30-60 parts of plant extract and 0.5-2 parts of surfactant; the plant extract is obtained by mixing Merremia boisiana (Gagn.) v. Ooststr. extract, Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb. extract according to the volume ratio of 4-8: 2-5: 2-5. The invention also provides a preparation method of the composition and an application method in preventing and treating asparagus stem blight. The composition provided by the invention has the advantages of simple and cheap raw materials, environmental protection, no secondary pollution to the agricultural growth environment, effective prevention and treatment of asparagus stem blight, and remarkable improvement of asparagus yield and quality.
Description
DESCRIPTION LU504686
COMPOSITION FOR PREVENTING AND TREATING ASPARAGUS STEM
BLIGHT AND PREPARATION METHOD THEREOF
The invention belongs to the technical field of agricultural biological control, in particular to a composition for preventing and treating asparagus stem blight and a preparation method thereof.
Asparagus (Asparagus officinalis L.) is a seedling of asparagus, a perennial herb of
Asparagaceae, which can be eaten by vegetables. Asparagus has extremely high nutritional value, and is rich in vitamin B, vitamin A, folic acid, selenium, iron, manganese, zinc and other trace elements, and has various amino acids necessary for human body.
The selenium content of asparagus is higher than that of ordinary vegetables, close to that of selenium-rich mushrooms, even comparable to that of marine fish and shrimp, and it has the functions of moistening lung, relieving cough, eliminating phlegm, inhibiting tumor growth, etc. It is deeply loved by people and enjoys the reputation of "king of vegetables".
In recent years, with the expansion of asparagus cultivation area, the occurrence of diseases has been aggravated year by year, especially the occurrence and harm of stem blight, which has seriously affected the yield and quality of asparagus. Asparagus stem blight is caused by fungi, and the pathogen is Phomopsis asparagi. Asparagus stem blight spreads rapidly in rainy and windy conditions, and it can also be infected by rainwater splashing. Air infection is the main cause of large-scale disease, and the direction of field spread and the rapid onset are often affected by wind.
At the same time, low-lying, sticky soil, too much nitrogen fertilizer, etc., are easy td/504686 aggravate the disease. Asparagus stem blight will lead to poor growth and development of asparagus, reduce yield and quality, even die ahead of schedule and destroy the whole field. At present, the control of asparagus stem blight is mainly based on chemical control. However, due to the extensive use of chemical agents for many years, the pathogen has strong drug resistance in many areas, which greatly reduces the effect of pesticides, and the use of a large number of chemical agents will also cause environmental pollution, pesticide residues and other problems. Therefore, it is one of the key problems to be solved in this field to find green and environmentally friendly chemicals that can effectively control asparagus stem blight.
In view of this, the invention aims to provide a composition for preventing and treating asparagus stem blight, which can effectively prevent and treat asparagus stem blight and improve the yield and quality of asparagus.
In order to achieve the above object, the present invention provides the following technical scheme.
The present invention provides a composition for preventing and treating asparagus stem blight. The composition consists of 30-60 parts of plant extract and 0.5-2 parts of surfactant in parts by weight. The plant extract is obtained by mixing Merremia boisiana (Gagn.) v. Ooststr. extract, Artemisiacapillaris Thunb. extract and Houttuynia cordata
Thunb. extract according to the volume ratio of 4-8: 2-5: 2-5.
Preferably, the composition consists of 41-54 parts of plant extract and 0.9-1.4 parts of surfactant in parts by weight.
Preferably, the plant extract is obtained by mixing Merremia boisiana (Gagn.) v.
Ooststr. extract, Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb. extract according to the volume ratio of 5.2-6.7: 3.1-4.4: 3.4-4 2.
Preferably, the preparation method of the Merremia boisiana (Gagn.) v. OostskU504686 extract comprises the following steps: pulverizing Merremia boisiana (Gagn.) v. Ooststr., adding 80% ethanol solution according to the material-liquid ratio of 1: 8-20 g/mL for reflux extraction, and filtering the extract to obtain the filtrate which is Merremia boisiana (Gagn.) v. Ooststr. extract.
Preferably, the preparation method of the Artemisiacapillaris Thunb. extract comprises: pulverizing Artemisiacapillaris Thunb., adding water according to the ratio of material to liquid of 1: 12-24 g/mL, boiling with strong fire, decocting with slow fire for 2-6 h, and filtering to remove residues, thus obtaining the filtrate as Artemisiacapillaris Thunb. extract.
Preferably, the preparation method of the Houttuynia cordata Thunb. extract comprises the following steps: pulverizing Houttuynia cordata Thunb., adding water according to the material-liquid ratio of 1: 10-18 g/mL, adding cellulase and xylanase, performing enzymolysis for 10-13 h under the condition of ultrasonic assistance, and filtering after enzymolysis to obtain the filtrate which is Houttuynia cordata Thunb. extract.
Preferably, the addition amount of cellulase is 3-5% of the mass of Houttuynia cordata Thunb., and the addition amount of xylanase is 2-4% of the mass of Houttuynia cordata Thunb. .
The present invention also provides a preparation method of the above-mentioned composition, comprising the following steps: mixing the Merremia boisiana (Gagn.) v.
Ooststr. extract, Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb. extract in proportion, adding surfactant, and stirring and mixing uniformly.
The present invention also provides a method for preventing and treating asparagus stem blight, including diluting the above-mentioned composition to 50-200 times of diluent for spraying.
Preferably, the diluent is used in an amount of 300-1000 mL/hm? each time.
The invention has the beneficial effects that:
According to the invention, the Merremia Boisiana (gagn.) v. ooststr. extradtU504686
Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb. extract are reasonably proportioned, so that the active ingredients of the three extract work together, and the effect of rapidly inhibiting asparagus stem blight can be exerted, and the recurrence of the stem blight can be inhibited for a long time, so as to effectively improve the yield and quality of asparagus.
The invention uses the bacteriostatic effect of plant extract to prevent and treat asparagus stem blight, does not need to use a large number of chemical reagents, and the product is green and environment-friendly, without any secondary pollution to the environment, at the same time, the pesticide residue in the asparagus planting process is reduced, and the food safety and planting benefit of asparagus are significantly improved.
The present invention provides a composition for preventing and treating asparagus stem blight. The composition consists of 30-60 parts of plant extract and 0.5-2 parts of surfactant in parts by weight. The plant extract is obtained by mixing Merremia boisiana (Gagn.) v. Ooststr. extract, Artemisiacapillaris Thunb. extract and Houttuynia cordata
Thunb. extract according to the volume ratio of 4-8: 2-5: 2-5.
The composition disclosed by the invention only contains plant extract and surfactant, and the active ingredients in Merremia boisiana (Gagn.) v. Qoststr.,
Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb. extract are reasonably proportioned, so that the efficacy of effectively preventing and controlling asparagus stem blight is achieved, and pesticide residues in asparagus are avoided.
In the present invention, the composition is preferably composed of 41-54 parts of plant extract and 0.9-1.4 parts of surfactant, more preferably 47-51 parts of plant extract and 1.1-1.2 parts of surfactant.
The invention does not limit the choice of specific surfactants. The surfactant added/504686 in the invention can effectively improve the adhesion of plant extract on the surface of asparagus stems, and improve the inhibition/killing effect of active components in plant extract on asparagus stem blight pathogens.
In the invention, the mixture ratio of the plant extract is preferably Merremia boisiana (Gagn.) v. Qoststr. extract: Artemisiacapillaris Thunb. extract: Houttuynia cordata Thunb. extract is obtained by mixing according to the volume ratio of 5.2-6.7: 3.1-4.4: 3.4-4 2.
More preferably, Merremia boisiana (Gagn.) v. Ooststr. extract: Artemisiacapillaris Thunb. extract: Houttuynia cordata Thunb. extract is mixed according to the volume ratio of 5.8-6.2: 3.5-3.8: 3.8-4.0.
In the present invention, the Merremia boisiana (Gagn.) v. Qoststr. extract,
Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb. extract are respectively extracted from Merremia boisiana (Gagn.) v. Ooststr, whole herb,
Artemisiacapillaris Thunb. whole herb and Houttuynia cordata Thunb. whole herb.
Merremia boisiana (gagn.) v. Qoststr. is a large twining herb or subshrub of convolvulaceae. Merremia boisiana (gagn.) v. Qoststr. contains a large number of secondary metabolites such as terpenoids, alcohols, esters and ketones, which have obvious allelopathy on many plants.
The preparation method of Merremia boisiana (gagn.) v. Ooststr. extract of the invention comprises the following steps: pulverizing Merremia boisiana (gagn.) v.
Ooststr., adding 80% ethanol solution according to the material-liquid ratio of 1: 8-20 g/mL for reflux extraction, and filtering the extract to obtain the filtrate, namely Merremia boisiana (gagn.) v. Ooststr. extract. The Merremia boisiana (gagn.) v. Qoststr. is preferably sieved with 5-10 meshes after being crushed. The material-liquid ratio is preferably 1: 12-15g /mL, more preferably 1: 13-13.5 g/mL. The reflux extraction temperature of the invention is 75-85°C, preferably 79-82°C. The reflux extraction time is 30-50 min, preferably 39-43 min.
According to the invention, the Merremia boisiana (gagn.) v. Qoststr. whole hetkJ504686 extract prepared by the method can effectively inhibit the growth of pathogenic bacteria of asparagus stem blight, and can achieve a long-term bacteriostatic effect when sprayed on the surface of asparagus stems without affecting the growth of asparagus.
Artemisiacapillaris Thunb. is a plant belonging to the genus Oregano, Compositae, also known as Oregano, Baihao, Rat's Paw, Tian Naili, etc. Artemisiacapillaris Thunb. can be used as medicine, which has the functions of clearing away heat and promoting diuresis, relieving fever and inflammation, expelling worms and inhibiting bacteria, and can effectively inhibit the growth of various bacteria or fungi.
The preparation method of Artemisiacapillaris Thunb. extract comprises the following steps: pulverizing Artemisiacapillaris Thunb., adding water according to the material-liquid ratio of 1: 12-24 g/mL, boiling with strong fire, decocting with slow fire for 2-6 h, and filtering to remove residues, thus obtaining the filtrate which is
Artemisiacapillaris Thunb extract. The Artemisiacapillaris Thunb. is preferably sieved with 5-10 meshes after being crushed. The material-liquid ratio is preferably 1: 14-21 g/mL, more preferably 1: 17-18 g/MI. The time for decocting with slow fire is preferably 3.5-45h.
According to the invention, the Artemisiacapillaris Thunb. extract prepared by the method can effectively kill the pathogenic bacteria of asparagus stem blight and inhibit the regeneration of the pathogenic bacteria, thereby effectively inhibiting the recurrence of the stem blight.
Houttuynia cordata Thunb. is a plant belonging to Saururaceae and Water Shredded
Genus, also known as Cencao, Ji, Houttuymia Herb, mainly contains volatile oil, decanoyl acetaldehyde Houttuynia cordata Thunb. and other components, which has obvious inhibitory effect on a variety of pathogenic bacteria.
The preparation method of the Houttuynia cordata Thunb. extract comprises tH&J504686 following steps: pulverizing Houttuynia cordata Thunb., adding water according to the material-liquid ratio of 1: 10-18 g/mL, adding cellulase and xylanase, performing enzymolysis for 10-13 h under the condition of ultrasonic assistance, and filtering after enzymolysis to obtain the filtrate which is Houttuynia cordata Thunb. The Houttuynia cordata Thunb. is preferably sieved by 5-10 meshes after being crushed. The material-liquid ratio is preferably 1: 13-17 g/mL, more preferably 1: 15-16 g/mL. The addition amount of the cellulase is 3-5%, preferably 3.6-4.1% of the mass of Houttuynia cordata Thunb. The addition amount of xylanase is 2-4%, preferably 2.8-3.2% of the mass of Houttuynia cordata Thunb. The enzymolysis time is preferably 11.5-12 h. The enzymolysis temperature of the invention is 55-62°C, preferably 57-59°C. The present invention is not limited to the specific ultrasound equipment.
According to the invention, the Houttuynia cordata Thunb. extract prepared by the above method interacts with the active ingredients in the Merremia boisiana (Gagn.) v.
Ooststr. extract and the Artemisiacapillaris Thunb. extract, so that the effect of quickly inhibiting asparagus stem blight can be achieved, and the recurrence of asparagus stem blight can be inhibited for a long time, so that the effect of effectively improving the yield and quality of asparagus can be achieved.
The invention also provides a preparation method of the composition, which comprises the following steps: mixing the Merremia boisiana (Gagn.) v. Qoststr. extract, the Artemisiacapillaris Thunb extract and the Houttuynia cordata Thunb. extract in proportion, adding the surfactant, and stirring and mixing uniformly. The invention does not limit the specific stirring mode and stirring parameters.
The invention also provides a method for preventing and treating asparagus stem blight, which comprises the following steps: diluting the composition into 50-200 times of diluent for spraying, where the dilution ratio is preferably 80-120 times.
According to the invention, the liquid medicine can be sprayed once in advandé/504686 during the peak period of asparagus stem blight, so as to achieve the effect of preventing asparagus stem blight. The invention can also spray the liquid medicine at the onset of asparagus stem blight once every 5-7 days, so as to achieve the effect of effectively treating asparagus stem blight.
The use amount of the diluent in the invention is 300-1000 mL/hm?, preferably 400-600 mL/hm°.
When the composition liquid medicine is used, the usage amount and spraying times can be appropriately increased or decreased according to the severity of asparagus stem blight.
The technical solutions provided by the present invention will be described in detail with examples below, but they cannot be understood as limiting the protection scope of the present invention.
Example 1
This example provides a composition for preventing and treating asparagus stem blight: (1) preparing Merremia boisiana (Gagn.) v. Qoststr. extract. pulverizing Merremia boisiana (Gagn.) v. Ooststr., sieving with a 5-mesh sieve, adding 80% ethanol solution according to the material-liquid ratio of 1:13 g/mL, reflux extracting at 80°C for 40 min, filtering the obtained extract, and filtrate for later use. (2) preparing Artemisiacapillaris Thunb. Extract: after pulverizing Artemisiacapillaris
Thunb., sieving with a 5-mesh sieve, adding water according to the ratio of material to liquid of 1:18 g/mL, boiling with strong fire, then decocting with slow fire for 4 h, and filtering to remove residues, so as to obtain filtrate for later use; (3) preparing Houttuynia cordata Thunb. extract: after pulverizing Houttuynia cordata Thunb., adding water according to the material-liquid ratio of 1:16 g/mL, then adding cellulase being 4% of Houttuynia cordata Thunb. by mass and xylanase being 3% of Houttuynia cordata Thunb. by mass, and performing enzymolysis at 58°C for 12 h under the assistance of ultrasonic wave, and then filtering the filtrate for later use;
(4) mixig 60 ml of Merremia boisiana (Gagn.) v. Qoststr. extract, 35 ml bE504686
Artemisiacapillaris Thunb. extract and 40 ml of Houttuynia cordata Thunb. extract respectively, then adding 2.7 g of glycerol and mixing evenly.
Example 2
The only difference between this example and Example 1 is that 70 ml of Merremia boisiana (Gagn.) v. Qoststr. extract, 38 ml of Artemisiacapillaris Thunb. extract and 42 ml of Houttuynia cordata Thunb. extract are respectively mixed, and then 4 g of glycerol is added, and the mixture is evenly stirred.
Example 3
The only difference between this example and Example 1 is that the glycerol is replaced by calcium dodecylbenzenesulfonate.
Comparative example 1
The only difference from Example 1 is that 81 ml of Merremia boisiana (Gagn.) v.
Ooststr. extract and 54 ml of Houttuynia cordata Thunb. extract are respectively mixed, then 2.7 g of glycerol is added, and the rest are the same as Example 1.
Comparative example 2
The only difference from Example 1 is that 63 ml of Artemisiacapillaris Thunb. extract and 72 ml of Houttuynia cordata Thunb. extract are respectively mixed, and then 2.7 g of glycerol is added, and the rest are the same as Example 1.
Comparative example 3
The only difference from Example 1 is that 85 ml of Merremia boisiana (Gagn.) v.
Ooststr. extract and 50 ml of Artemisiacapillaris Thunb. extract are respectively mixed, then 2.7 g of glycerol is added, and the rest are the same as Example 1.
Comparative example 4
The only difference from Example 1 is that perilla frutescens extract is used instead of Houttuynia cordata Thunb., and the rest are the same as Example 1. The preparation method of perilla frutescens extract includes: pulverizing perilla frutescens, sieving with a 5-mesh sieve, adding water according to the ratio of material to liquid of 1:16 g/mL, adding 4% of cellulase and 3% of xylanase based on the mass of perilla frutescens,
performing enzymolysis at 58°C for 12 h with the assistance of ultrasonic wave, filtering/504686 after enzymolysis, and keeping the filtrate for later use.
Comparative example 5
The only difference from Example 1 is that the extract of Radix Peucedani is used instead of the Merremia boisiana (Gagn.) v. Ooststr. extract, and the rest are the same as Example 1. The method for preparing the extract of Radix Peucedani comprises pulverizing Radix Peucedani, sieving with a 5-mesh sieve, adding water according to the ratio of material to liquid of 1:16 g/mL, adding 4% of cellulase and 3% of xylanase by mass of Radix Peucedani, performing enzymolysis at 58°C for 12 h with the assistance of ultrasonic wave, filtering after enzymolysis, and keeping the filtrate for later use.
Comparative example 6
The only difference from Example 1 is that 60 ml of Merremia boisiana (Gagn.) v.
Ooststr. extract, 35 ml of Artemisiacapillaris Thunb. extract and 40 ml of Houttuynia cordata Thunb. extract are respectively mixed and stirred evenly.
Comparative example 7
The only difference from Example 1 is that 60 ml of Merremia boisiana (Gagn.) v.
Ooststr. extract, 35 ml of Artemisiacapillaris Thunb. extract and 40 ml of Houttuynia cordata Thunb. extract are respectively mixed, and then 2.7 g of glycerol, 30 g of carbendazim (wettable powder) and 25 g of chitosan oligosaccharide (1600Da) are added and stirred and mixed.
Example 4
In this example, the effects of the liquid medicine prepared in Examples 1-3 and
Comparative Examples 1-7 on the control effect and yield increase index of asparagus stem blight were explored by the experimental method described in the article "Control
Effect of Six Fungicides on Asparagus Stem Blight" by Yang Yingqing and Lan Bo. 1. Experimental design
Each test plot covers an area of 30 m?, and each agent is diluted to 100 times solution with the dosage of 600 g/hm?; taking clean water treatment as control. There were 11 treatments, 3 replicates and random block arrangement.
2. Disease investigation LU504686
Five diagonal sampling points were used in each plot, and five connected asparagus were taken at each point. Three asparagus were randomly investigated in each asparagus cluster, and 75 asparagus were investigated in 25 asparagus clusters, and the number of healthy plants and diseased plants at all levels were recorded. The grading standard of illness is: grade 0: no disease; grade 1: the incidence area accounts for less than 6% of the total area; grade 2: the incidence area accounts for 6% - 10% of the total area; grade 3: the incidence area accounts for 11% - 20% of the total area; grade 4: the incidence area accounts for 21% - 30% of the total area; grade 5: the incidence area accounts for more than 30% of the total area. 3. Data processing calculation method
Disease index = > (number of diseased plants at all grades x relative grade value)/total number of plants investigated x5x100;
Control effect = (disease index of control area)/disease index of control area x 100%;
Yield-increasing rate = (output of treatment area and blank control area)/output of blank control area x 100%. 4. Experimental results
The average value of three repeated experiments is counted, and the results are shown in Table 1.
Table 1 Effects of different compositions on control of stem blight and yield increadé/504686 index
Group Control effect (100%) Yield increase rate (100%)
Example 1 95.12+0.47 20.46+0.81
Example 2 94.84+0.72 21.57+0.29
Example 3 94.81+0.35 20.74+0.72
Comparative example 1 71.05+0.89 6.15+0.89
Comparative example 2 70.85+0.46 7.58+0.56
Comparative example 3 60.81+1.03 6.89+0.43
Comparative example 4 16.56+0.58 1.08+1.03
Comparative example 5 62.15+0.94 5.98+0.74
Comparative example 6 60.17+0.56 6.04+0.42
Comparative example 7 90.89+0.41 15.84+0.87
It can be seen from Table 1 that the control effects of the composition liquid prepared in Examples 1 to 3 on asparagus stem blight are all above 94%, and all of them can increase asparagus yield by more than 20%. It shows that the composition prepared by the invention has a good control effect on asparagus stem blight.
In Comparative Examples 1-3, the addition of control effect extract, Merremia boisiana (Gagn.) v. Qoststr. extract and Houttuynia cordata Thunb. extract were abandoned respectively, but the total plant extract content was not reduced, and the control effect of the obtained composition on asparagus stem blight was reduced, and the yield increase rate of asparagus was only 6-7%. It shows that the effective components in Artemisiacapillaris Thunb. extract, Merremia boisiana (Gagn.) v. Qoststr. extract and Houttuynia cordata Thunb. extract are reasonably proportioned, and the synergistic effect of the three components achieves good control effect and yield-increasing effect on asparagus stem blight.
In Comparative Examples 4-5, perilla frutescens extract and Radix PeucedahV504686 extract were used to replace Houttuynia cordata Thunb. extract and Merremia boisiana (Gagn.) v. Ooststr. respectively, and compared with the other two extracts, the control effect of the obtained composition on asparagus stem blight decreased, and the control effect of the composition obtained in Comparative Example 4 decreased significantly, with almost no control effect. It shows that not all plant extracts with bacteriostatic effect can be mixed to obtain the composition liquid medicine for effectively preventing and treating asparagus stem blight. The invention reasonably proportionates
Artemisiacapillaris Thunb. extract, Merremia boisiana (Gagn.) v. Ooststr. extract and
Houttuynia cordata Thunb. extract, so that the three extracts have synergistic effect and do not affect each other, thus achieving good prevention and control effect and yield-increasing effect on asparagus stem blight.
In Comparative Example 6, the addition of surfactant was omitted, and the control effect of the obtained composition on asparagus stem blight decreased, indicating that the surfactant improved the control effect on asparagus stem blight by increasing the residence time and adhesion ability of plant extract on asparagus surface.
In Comparative Example 7, chitosan oligosaccharide and carbendazim, which can kill a variety of bacteria and fungi, were added in addition to plant extract and surfactant.
The control effect of the obtained composition on asparagus stem blight was not much different from that of Examples 1-3, but slightly lower than that of Examples 1-3. It is shown that the control effect and yield increase effect of asparagus stem blight are obviously improved by the reasonable ratio of Artemisiacapillaris Thunb. extract,
Merremia boisiana (Gagn.) v. Qoststr. extract, Houttuynia cordata Thunb. extract and surfactant, and no additional sterilization raw materials need to be added.
The above is only the preferred embodiment of the present invention, and it should be pointed out that a person skilled in the art can make several improvements and embellishments without departing from the principle of the present invention, and these improvements and embellishments should also be regarded as the protection scope of the present invention.
Claims (10)
- CLAIMS LU5046861. A composition for preventing and treating asparagus stem blight, characterized in that the composition consists of 30-60 parts of plant extract and 0.5-2 parts of surfactant in parts by weight; the plant extract is obtained by mixing Merremia boisiana (Gagn) v; Ooststr; extract, Artemisiacapillaris Thunb, extract and Houttuynia cordata Thunb; extract according to the volume ratio of 4-8: 2-5: 2-5.2. The composition according to claim 1, wherein the composition consists of 41-54 parts of plant extract and 0.9-1.4 parts of surfactant in parts by weight.3. The composition according to claim 1, characterized in that the plant extract is obtained by mixing Merremia boisiana (Gagn) v; Ooststr; extract, Artemisiacapillaris Thunb. extract and Houttuynia cordata Thunb, extract according to the volume ratio of5.2-6.7: 3.1-4.4: 3.4-4 2.4. The composition according to claim 1, characterized in that the preparation method of the Merremia boisiana (Gagn) v; Ooststr; extract comprises the following steps: pulverizing Merremia boisiana (Gagn) v; Ooststr, adding 80% ethanol solution according to the material-liquid ratio of 1: 8-20 g/mL for reflux extraction, and filtering the extract to obtain the filtrate which is Merremia boisiana (Gagn) v; Ooststr; extract.5. The composition according to claim 1, characterized in that the preparation method of the Artemisiacapillaris Thunb; extract comprises: pulverizing Artemisiacapillaris Thunb, adding water according to the ratio of material to liquid of 1: 12-24 g/mL, boiling with strong fire, decocting with slow fire for 2-6 h, and filtering to remove residues, thus obtaining the filtrate as Artemisiacapillaris Thunb, extract.6. The composition according to claim 1, characterized in that the preparatidi/504686 method of the Houttuynia cordata Thunb; extract comprises the following steps: pulverizing Houttuynia cordata Thunb, adding water according to the material-liquid ratio of 1: 10-18 g/mL, adding cellulase and xylanase, performing enzymolysis for 10-13 h under the condition of ultrasonic assistance, and filtering after enzymolysis to obtain the filtrate which is Houttuynia cordata Thunb; extract.7. The composition according to claim 6, wherein the addition amount of cellulase is 3-5% of the mass of Houttuynia cordata Thunb, and the addition amount of xylanase is 2-4% of the mass of Houttuynia cordata Thunb.8. A preparation method of the composition according to any one of claims 1 to 7, characterized by comprising the following steps: mixing the Merremia boisiana (Gagn) v; Ooststr; extract, Artemisiacapillaris Thunb, extract and Houttuynia cordata Thunb; extract in proportion, adding surfactant, and stirring and mixing uniformly.9. A method for preventing and treating asparagus stem blight, characterized in that the composition according to any one of claims 1 to 7 is diluted to 50-200 times of diluent for spraying.10. The method according to claim 9, wherein the diluent is used in an amount of 300-1000 mL/hm? each time.
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