CN114988653A - Aquaculture system and method for improving utilization rate of nitrogen and phosphorus and reducing emission of nitrogen and phosphorus - Google Patents

Aquaculture system and method for improving utilization rate of nitrogen and phosphorus and reducing emission of nitrogen and phosphorus Download PDF

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CN114988653A
CN114988653A CN202210849635.3A CN202210849635A CN114988653A CN 114988653 A CN114988653 A CN 114988653A CN 202210849635 A CN202210849635 A CN 202210849635A CN 114988653 A CN114988653 A CN 114988653A
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nitrogen
module
phosphorus
algae
aquaculture
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刘昊昆
刘翠
朱晓鸣
解绶启
韩冬
巩玉龙
金俊琰
张志敏
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Institute of Hydrobiology of CAS
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00Multistage treatment of water, waste water or sewage
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/045Filters for aquaria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/001Processes for the treatment of water whereby the filtration technique is of importance
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/14NH3-N
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/15N03-N
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/16Total nitrogen (tkN-N)
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention discloses an aquaculture system and method for improving the bioavailability of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus, wherein the system comprises a culture module, a bacterium treatment module and an algae culture module which are sequentially connected through pipelines; a return pipeline is arranged between the algae cultivation module and the cultivation module. Conveying the culture water in the culture module to a bacteria treatment module, and treating the effluent water through a filtering device to enter an algae culture module; the water flow entering the algae cultivation module enters the cultivation module through the return pipeline after passing through the algae growth substrate for attaching and growing algae. According to the invention, by utilizing a modular design, low-nutrition level organisms are integrated into an aquaculture system, the content of ammonia nitrogen, nitrite, nitrate and total nitrogen in the aquaculture water body is reduced, the water quality of the aquaculture water body is improved, the aquaculture discharge is obviously reduced, the yield of biomass is increased through algae, and the resource utilization rate is increased.

Description

Aquaculture system and method for improving utilization rate of nitrogen and phosphorus and reducing emission of nitrogen and phosphorus
Technical Field
The invention relates to the technical field of aquaculture, in particular to an aquaculture system and method for improving utilization rate of nitrogen and phosphorus and reducing emission of nitrogen and phosphorus.
Background
In recent years, with the rapid development of aquaculture industry, the density and the intensification degree of aquaculture are increasing, and the supply quantity of aquatic products is also increasing rapidly. However, the high-density intensive culture not only brings serious load to the water environment, but also gradually reduces the quality of aquatic products, and is not beneficial to the sustainable development of aquaculture. Because of the low efficiency of the utilization of the relevant resources by a single aquaculture mode, the discharge of the residual baits and the nutrients such as nitrogen and phosphorus which are not utilized in the animal excreta in the aquaculture process into water can cause a series of environmental problems. Statistically, only about 20% to 30% of the nitrogen and phosphorus in the feed will be directly utilized by the aquatic animals, and the remaining nitrogen (68% to 86%) is discharged into the water. Therefore, the development of corresponding technology realizes resource utilization of the water culture eutrophic waste, is beneficial to improving the production efficiency of aquaculture, can reduce the influence on the environment, and has very important significance.
In the prior art, the treatment processes of chemical agent addition, biological sludge and the like are mostly adopted to carry out nitrogen and phosphorus removal on high-nitrogen and phosphorus wastewater of aquaculture, although the water body is purified, resources such as ammonia, nitrogen, phosphorus and the like cannot be reasonably utilized, the treatment process is complex and high in cost, professional equipment needs to be independently added for treatment, and the production process is reduced.
Therefore, how to provide a simple and low-cost aquaculture method is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides an intensive multi-nutrient aquaculture mode, which reduces nitrogen and phosphorus emission, improves the utilization rate and produces high value-added products.
In order to achieve the purpose, the invention adopts the following technical scheme:
firstly, the invention provides an aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus, which comprises a culture module, a bacteria treatment module and an algae culture module which are sequentially connected through pipelines;
a return pipeline is arranged between the algae cultivation module and the cultivation module.
Furthermore, an illumination system is arranged in the cultivation module, so that illumination conditions in the cultivation module can meet cultivation requirements conveniently.
Furthermore, the bacteria treatment module comprises a filter box and a bacteria culture substrate, the bacteria culture substrate adopts polyurethane sponge biological filler, the cost is low, bacteria grow on the filter cotton to form a carrier with microorganisms, and the bacteria treatment module has better interception and filtration effects.
Preferably, the algae cultivation module comprises an incubator, a fixing frame arranged in the incubator, illumination modules arranged on two sides of the fixing frame, and algae growth substrates arranged on the fixing frame, wherein the top end of the incubator is provided with a water inlet, the bottom end of the incubator is provided with a water outlet, the water inlet is communicated with the bacteria treatment module, and the water outlet is communicated with the return pipeline.
Further, the algae growth substrate is a polyarylate net with the pore diameter of 2 mm.
In addition, the invention also provides a culture method of the aquaculture system, which adopts the technical scheme and improves the bioavailability of nitrogen and phosphorus and reduces the emission of nitrogen and phosphorus, and the culture method comprises the following steps:
the method comprises the following steps: conveying the culture water in the culture module to a bacteria treatment module, and filtering the culture water to obtain effluent which enters an algae culture module;
step two: the water flow entering the algae cultivation module enters the cultivation module through the return pipeline after passing through the algae growth substrate for attaching and growing algae.
Preferably, in the step one, a circulating pump is adopted to convey the culture water in the culture module to the bacteria treatment module.
Preferably, the water temperature in the culture box is 26-28 ℃, the dissolved oxygen is more than 7.5mg/L, the pH is 6.4-7.3, and the illumination period is 12L/12D.
Preferably, the water flow rate in the algae culture box is 1L/min, and the illumination period is 18L/6D.
Preferably, the algae in the algae culture module is cyanophyta microalgae, more preferably Oscillatoria microalgae, and the introduced algae culture module can not only harvest microalgae biomass to improve the utilization rate of nutrient substances and reduce the discharge of nitrogen and phosphorus in solid waste, but also can obviously increase the biomass of Acidobacterium (Acidobacterium) in the bacteria treatment module, promote the conversion of nitrate and nitrite and reduce the content of nitrate and nitrite in a water body.
Preferably, the algae inoculation concentration in the algae cultivation module is more than 10e 6/mL, and the inoculation amount is 30 mL.
According to the technical scheme, compared with the prior art, the invention discloses an aquaculture system and method for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus, and the aquaculture system and method have the following beneficial effects:
the constructed aquaculture system comprising the culture module, the bacteria treatment module and the algae culture module can improve circulating water quality and reduce water treatment difficulty, and low-nutrition-level organisms are integrated into the aquaculture system, nitrogen and phosphorus in an aquaculture water body are fully and efficiently utilized, the yield of biomass is improved, the resource utilization rate is improved, the microbial biomass of Acetobacter phylum in the bacteria treatment module can be obviously increased through preferably introducing algae, the ammonia nitrogen content, the nitrite content, the nitrate content, the total nitrogen content and the total phosphorus content of the aquaculture water body are reduced, and the water quality of the aquaculture water body is improved. After the algae culture module is introduced, the bioavailability of nitrogen and phosphorus of the whole recirculating aquaculture system is respectively improved by 38.81 percent and 93.79 percent, the discharge of nitrogen and phosphorus in the aquaculture process is respectively reduced by 10.24 percent and 17.78 percent, the recycling of nitrogen and phosphorus is favorably improved, and the sustainable development of aquaculture is promoted.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is an overall structure diagram of an aquaculture system of the present invention for improving the utilization of nitrogen and phosphorus and reducing the discharge of nitrogen and phosphorus;
in the figure, 1-culture module, 11-circulating pump, 12-lighting system, 2-bacteria processing module, 21-filter tank, 22-bacteria culture medium, 3-algae culture module, 31-incubator, 32-fixing frame, 33-lighting module and 4-return pipe.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in the attached figure 1, the aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus comprises a culture module 1, a bacteria treatment module 2 and an algae culture module 3 which are sequentially connected through pipelines;
a return pipeline 4 is arranged between the algae cultivation module 3 and the cultivation module 1.
The cultivation module 1 is internally provided with an illumination system 12, the bacteria treatment module 2 comprises a filter box 21 and a bacteria culture substrate 22, the concrete substrate adopts polyurethane sponge biological filler, the algae culture module 3 comprises an incubator 31, a fixing frame 32 arranged in the incubator 31, illumination modules 33 arranged on two sides of the fixing frame 32 and algae growth substrates (polyarylate net with the aperture of 2 mm) arranged on the fixing frame 32, the top end of the incubator 31 is provided with a water inlet, the bottom end of the incubator 31 is provided with a water outlet, the water inlet is communicated with the filter box 21 of the filter device 2, and the water outlet is communicated with the return pipeline 4.
The aquaculture method of the aquaculture system for improving the bioavailability of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus, which adopts the technical scheme, comprises the following steps:
the method comprises the following steps: conveying the culture water in the culture module 1 to the bacteria treatment module 2 by adopting a circulating pump 11, treating the effluent by a filter cotton layer 22 in the filter device 2, and feeding the effluent into the algae culture module 3;
step two: the water flow entering the algae cultivation module 3 passes through the fixing frame 32 in the algae cultivation box 31, and is converted by the algae growing on the algae growth substrate on the fixing frame 32 and then enters the cultivation module 1 through the return pipeline 4.
Wherein, the algae in the algae cultivation module 3 is cyanophyta microalgae, and more preferably Oscillatoria microalgae.
The system and the culture method of the invention are verified in the following by using specific embodiments:
the experimental group adopts the culture system and method of the invention, and simultaneously sets a culture system without an algae culture module as a control group, each treatment is set to be 3 times, 20 fishes (the initial weight is 19.2 +/-0.2 g) are cultured in each culture box, and the same feed is fed at 8:30 and 16:30 every day during the experimental period. The volume of the culture box is 400L, the volume of the filtering device is 30L, and the microalgae is cultured by flowing water at the flow rate of 1L/min. During the culture period, the water temperature is 27 +/-1 ℃, the dissolved oxygen is more than 7.5mg/L, the pH value is 6.4-7.3, the illumination period of the culture module is 12L/12D, the illumination period of the algae culture module is 18L/6D, the culture period of fish is 61 days, and the culture period of algae is 70 days.
The experimental culture of microalgae is Oscillatoria Oscillatoria sp, which is purchased from freshwater algae seed bank of aquatic organism research institute of Chinese academy of sciences, the inoculation concentration of the microalgae is more than 10e6 per mL, and each microalgae culture system is inoculated with 30 mL.
The results of the experiment are shown in tables 1-2 below:
table 1: results of growth and nitrogen and phosphorus utilization of cultured fish and microalgae in control group and experimental group
Figure BDA0003752939910000051
Figure BDA0003752939910000061
Table 2: water quality results of control group and experimental group
Control group Experimental group Experiment group reduces
Water ammonia nitrogen (mg/L) 1.35 0.98 27.41%
Nitrite in water (mg/L) 0.23 0.15 34.78%
Nitrate in water (mg/L) 15.09 11.53 23.59%
Total nitrogen (mg/L) of water body 20.65 17.00 17.68%
After the fish culture experiment is finished, the growth of the experimental fish of the two culture systems has no significant difference, and the feed efficiency of the cultured fish introduced into the algae culture module is improved by 24.66 percent. At the same time, 46.16 g of microalgae dry matter is harvested, and the crude protein content and the crude fat content of the microalgae are respectively 54.25 percent and 2.05 percent.
The deposition rates of nitrogen and phosphorus in the cultured fish bodies of the experimental groups introduced into the algae culture module are respectively improved by 8.34 percent and 30.68 percent, the deposition rates of nitrogen and phosphorus in the algae are 6.36 percent and 10.06 percent, the total bioavailability of nitrogen and phosphorus is respectively improved by 38.81 percent and 93.79 percent, the discharge of nitrogen and phosphorus in the culture process is respectively reduced by 10.24 percent and 17.78 percent, and the contents of ammonia nitrogen, nitrite, nitrate and total nitrogen in the water body are respectively reduced by 27.41 percent, 34.78 percent, 23.59 percent and 17.68 percent.
Meanwhile, Acidobacterium phylum (Acidobacterium) in the bacterial treatment module of the experimental group introduced into the algae culture module was significantly increased. Acidobacterium phylum is acidophilic bacteria, and the acidophilic bacteria have important functions in an ecological system and can promote the conversion of nitrate and nitrite and reduce the content of the nitrate and nitrite. In addition, acidophyla has a negative correlation with nitrogen.
In conclusion, the introduction of the algae culture module into the circulating water culture system can obviously increase the microbial biomass of the Acidobacterium door in the bacteria treatment module, reduce the contents of ammonia nitrogen, nitrite, nitrate and total nitrogen in the culture water body, and improve the water quality of the culture water body. After the algae culture module is introduced, the total bioavailability of nitrogen and phosphorus of the recirculating aquaculture system is respectively improved by 38.81 percent and 93.79 percent, the emission of nitrogen and phosphorus in the aquaculture process is respectively reduced by 10.24 percent and 17.78 percent, the recycling of nitrogen and phosphorus is favorably improved, the aquaculture emission is reduced, and the sustainable development of aquaculture is promoted.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

1. An aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus is characterized by comprising a culture module, a bacterium treatment module and an algae culture module which are sequentially connected through pipelines;
a return pipeline is arranged between the algae cultivation module and the cultivation module.
2. The aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to claim 1, wherein an illumination system is arranged in the aquaculture module.
3. The aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to claim 1, wherein the bacteria treatment module comprises a filter box and a bacteria culture medium.
4. The aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus of claim 1, wherein the algae cultivation module comprises an incubator, a fixed frame arranged in the incubator, illumination modules arranged on two sides of the fixed frame, and algae growth substrates arranged on the fixed frame, the incubator has a water inlet at the top end and a water outlet at the bottom end, the water inlet is communicated with the filtering device, and the water outlet is communicated with the return pipeline.
5. A culture method of an aquaculture system for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to any one of claims 1 to 4, which comprises the following steps:
the method comprises the following steps: conveying the culture water in the culture module to a bacteria treatment module, and filtering the culture water to obtain outlet water which enters an algae culture module;
step two: the water flow entering the algae cultivation module enters the cultivation module through the return pipeline after passing through the algae growth substrate for attaching and growing algae.
6. The aquaculture method for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to claim 5, wherein in the first step, the circulating pump is adopted to convey aquaculture water in the aquaculture module to the bacteria treatment module.
7. The aquaculture method for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to claim 5, wherein the water temperature in the aquaculture module is 26-28 ℃, the dissolved oxygen is more than 7.5mg/L, the pH value is 6.4-7.3, and the illumination period is 12L/12D.
8. The aquaculture method for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to claim 5, wherein the water flow rate in the algae cultivation module is 1L/min, and the illumination period is 18L/6D.
9. The method of claim 5, wherein the algae in the algae cultivation module are microalgae of the cyanophyta.
10. The aquaculture method for improving the utilization rate of nitrogen and phosphorus and reducing the emission of nitrogen and phosphorus according to claim 5, wherein the algae in the algae cultivation module is inoculated at a concentration of more than 10e6 seeds/mL and an inoculation amount of 30 mL.
CN202210849635.3A 2022-07-19 2022-07-19 Aquaculture system and method for improving utilization rate of nitrogen and phosphorus and reducing emission of nitrogen and phosphorus Pending CN114988653A (en)

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