CN114605030B - Method for recycling carbon-sink oxygen-release type cultivation sewage - Google Patents
Method for recycling carbon-sink oxygen-release type cultivation sewage Download PDFInfo
- Publication number
- CN114605030B CN114605030B CN202210153653.8A CN202210153653A CN114605030B CN 114605030 B CN114605030 B CN 114605030B CN 202210153653 A CN202210153653 A CN 202210153653A CN 114605030 B CN114605030 B CN 114605030B
- Authority
- CN
- China
- Prior art keywords
- microalgae
- biogas slurry
- algae
- denitrifying bacteria
- aerobic denitrifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/10—Temperature conditions for biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F7/00—Aeration of stretches of water
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention discloses a method for recycling carbon-sink oxygen-release type cultivation sewage, which comprises an aerobic denitrifying bacteria algae sewage front-end treatment system for enabling cultivation biogas slurry to meet microalgae cultivation requirements, and a method for cultivating microalgae and fixing CO by utilizing the treated biogas slurry 2 Is composed of two treatment stages of microalgae production system. The invention processes and utilizes the biogas slurry, the biogas slurry does not need to be diluted, the processed waste liquid of the farm does not need to be discharged with any liquid, the special discharge treatment for the residual liquid of the waste liquid of the farm is not needed, the reduction concentration of the sewage can be realized, the biodegradability of the biogas slurry is realized, no secondary pollution is generated, the effluent water from the sewage front-end processing system of aerobic denitrifying bacteria algae can fully play a role in the microalgae production system, the biogas can be used as a heat source, and the biogas combustion tail gas is recovered as CO 2 And the source is used for realizing the efficient production of microalgae.
Description
Technical Field
The invention relates to the technical field of waste treatment and ecological environmental protection, in particular to a method for recycling carbon-sink oxygen-releasing cultivation sewage.
Background
In recent years, with the rapid development of livestock and poultry raising industry, the discharge amount of livestock and poultry waste is rapidly increased, and the pollution load is high, so that the livestock and poultry waste becomes a main source of agricultural non-point source pollution, and the further development of the raising industry is severely restricted. With agriculture being an important source of global greenhouse gas emission, the total amount of livestock and poultry manure produced by the livestock and poultry industry is about 40 hundred million tons each year, which is also an important source of agriculture non-point source pollution, and the chemical oxygen demand of the livestock and poultry industry emission reaches 1268 ten thousand tons.
At present, the recycling of pig farm wastewater after the treatment of the breeding wastewater for fermentation to produce biogas is a common mode for the treatment of wastewater in the intensive pig farm at present, but the fermented biogas slurry still belongs to high-concentration organic wastewater and cannot reach the emission standard. The fermented biogas slurry treatment modes include an artificial wetland method, a biological pond method, MBR reactor treatment and the like, but the methods are difficult to be practically applied due to the problems of high cost and the like, so that the main mode of pig farm fermentation wastewater treatment is that the pig farm fermentation wastewater is directly discharged after simple treatment.
More and more expert scholars are focused on the research of 'pollution reduction and carbon reduction' synergy, but are often focused on the energy consumption part, and CO is generated in the process 2 Emissions tend to be neglected. The process mainly aims at a process flow for realizing the synergy of pollution reduction and carbon reduction in the high-concentration organic wastewater treatment process, and finally, the microorganism advanced treatment process is utilized to further reduce pollutants, realize tail water treatment, and simultaneously, synergistically increase carbon sink capacity, so that the whole treatment system basically reaches near zero carbon.
The aerobic denitrification bacterial algae symbiotic system has the characteristic of synchronously removing carbon, nitrogen and phosphorus, and symbiotic bacteria can decompose extracellular organic matters released by microalgae, but in the actual treatment process of biogas slurry, the fact that the organic matters in the biogas slurry are poor in biodegradability and directly used for microalgae production needs dilution is found, and the nutritional ingredient composition cannot directly meet the microalgae production.
At present, only single biogas slurry culture microalgae research, single microalgae treatment biogas slurry research and single sewage treatment research are carried out; a carbon fixation and oxygen release sewage treatment and microalgae production method which can integrate multiple technologies such as anaerobic fermentation, aerobic denitrification, carbon dioxide absorption, microalgae carbon dioxide fixation, biogas slurry treatment, microalgae production and the like do not exist.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for recycling carbon-sink oxygen-release type culture sewage.
The technical scheme of the invention is as follows: a method for recycling carbon-sink oxygen-releasing culture sewage includes such steps as preparing the sewage from the culture marsh liquid to meet the culture requirement of microalgae, treating the sewage by aerobic denitrifying bacteria, culturing microalgae, and fixing CO 2 Is composed of two treatment stages of microalgae production system.
Further, the method comprises the steps of:
step 1, a treatment stage of an aerobic denitrifying bacteria algae sewage front-end treatment system
S101, carrying out solid-liquid separation on the manure of the farm by a solid-liquid separation device, regulating the pH value of the separated waste liquid to 6.5-7.5, then introducing the waste liquid into an anaerobic fermentation tank, carrying out anaerobic fermentation to produce biogas, and then introducing the biogas slurry produced by anaerobic fermentation into a biogas slurry storage tank;
s102, controlling Chemical Oxygen Demand (COD) in biogas slurry in a biogas slurry storage tank in the step S101 to be less than 3000mg/L, and controlling solid suspended matter concentration (SS) to be less than 1000 mg/L;
S103, feeding the biogas slurry obtained in the step S102 into a algae symbiotic treatment device for treatment to form a culture solution meeting microalgae culture conditions;
s104, introducing the culture solution in the step S103 into microalgae production equipment, conveying the biogas in the step S101 to a biogas utilization system through a pipeline, generating power by using the biogas through the biogas utilization system, and generating waste heat and tail gas CO 2 Introducing into microalgae production equipment, and culturing microalgae;
s105, introducing the algae liquid produced by the microalgae production equipment in the step S104 into a harvesting equipment, separating by the harvesting equipment to form algae mud, drying and pulverizing by a drying equipment, and introducing the separated residual tail water into a algae symbiotic treatment equipment again to repeat the step S103.
Further, the step S101 uses acid liquor to adjust the pH of the separated sewage to 6.5-7.5, wherein the acid liquor is HCl solution and H 2 SO 4 In solutionBy adopting the method to adjust the pH of the plant manure, the plant manure can meet the condition of anaerobic fermentation in an anaerobic fermentation tank, and the economy is good.
Further, the apparatus for symbiotic treatment of a fungus in step S103 includes: the device comprises an aerobic denitrifying bacteria algae film group for fully degrading biogas slurry, a biological filter bed for adsorbing residual trace elements and suspended matters, an aeration tank for preprocessing and circulating water distribution, and a spray pipe for spraying the biogas slurry on the aerobic denitrifying bacteria algae film group, wherein the spray pipe is arranged above the aerobic denitrifying bacteria algae film group and is connected with the aeration tank through a pipeline and a water distribution pump, and the aerobic denitrifying bacteria algae film group, the biological filter bed and the aeration tank are sequentially connected in series from top to bottom;
wherein, the method of step S103 comprises the following steps:
1) Introducing the biogas slurry obtained in the step S102 into an aeration tank for nitrification treatment, and converting nitrogen-ammonia pollutants in the biogas slurry into non-volatile oxidized nitrate nitrogen through pretreatment nitrification reaction;
2) Inoculating aerobic denitrifying bacteria and microalgae on an aerobic denitrifying bacteria algae membrane group in advance, uniformly spraying the aerated biogas slurry on the aerobic denitrifying bacteria algae membrane group through a water distribution pump and a spray pipe for aerobic denitrification treatment, and fully degrading and converting the biogas slurry into N through the aerobic denitrifying bacteria and microalgae of the aerobic denitrifying bacteria algae membrane group 2 、CO 2 And water, meanwhile, water molecules in the biogas slurry are evaporated to the atmosphere through physical gasification by an aerobic denitrifying bacteria algae membrane group, and the breathing action of the aerobic denitrifying bacteria and microalgae can release water molecules generated by the aerobic denitrifying bacteria and microalgae to the atmosphere;
3) After partial nitrate nitrogen, phosphorus, potassium and other substances in the biogas slurry are metabolized and utilized by aerobic denitrifying bacteria and microalgae of an aerobic denitrifying bacteria algae membrane group, partial microelements and suspended matters are also filtered and adsorbed by a biological filter bed, the partial nitrate nitrogen, phosphorus, potassium and other substances are further metabolized and utilized by the aerobic denitrifying bacteria and microalgae of the biological filter bed, and the residual biogas slurry after treatment enters an aeration tank again;
4) Repeatedly circulating the biogas slurry in the step 1) -3) in the algae symbiotic treatment equipment, and finally forming a culture solution meeting microalgae culture conditions in an aeration tank;
through the algae symbiotic treatment equipment and the treatment method, the biogas slurry can be effectively treated to meet the microalgae culture condition, and the biogas slurry does not need to be diluted and discharged without liquid, so that the method has the advantages of high recycling utilization rate, low cost, environmental friendliness, almost no sludge generation, no liquid discharge and the like.
Further, in the step S104, the microalgae production equipment is an open type photobioreactor or a closed type photobioreactor, and a stirring device and/or an aeration device for keeping the algae liquid in a suspension state are arranged in the open type photobioreactor or the closed type photobioreactor; by using the microalgae production equipment, the culture solution, the waste heat generated after biogas power generation and the tail gas CO are efficiently utilized 2 Thereby realizing the high-efficiency production of microalgae, and having high utilization rate of resources and CO emission reduction 2 And the like.
Further, the microalgae is selected from at least one of chlorella, spirulina, scenedesmus, chlamydomonas and the like with stronger stress resistance, the algae is inoculated into the culture solution, and the algae solution is obtained by culture production in microalgae production equipment.
Further, in step S105, the harvesting device is any one of a precipitation device, a filtering device, or a centrifugal device; through above-mentioned harvesting equipment, can carry out the retrieval and utilization with the tail water after the microalgae production, treatment cost is low, and the operation is simple, can realize no liquid emission.
Further, the mass concentration of the algae liquid produced by the microalgae production equipment in the step S104 is 10-100 g/L; thereby being convenient for obtain algae mud and tail water through the solid-liquid separation of the harvesting equipment, having high utilization rate of resources and no liquid discharge.
Further, the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system can discharge partial water molecules in the biogas slurry to the atmosphere through natural evaporation or auxiliary evaporators, and are used for balancing the water quantity of the biogas slurry in the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system; therefore, the balance of water quantity in the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system is maintained, and biogas slurry is circularly treated and utilized for a plurality of times by matching with the microalgae production system, so that the waste of the farm treated by the method has no liquid discharge, and the method does not need to specially discharge the waste residual liquid of the farm, and has the advantages of simple and feasible process and no secondary pollution.
The beneficial effects of the invention are as follows:
(1) The method adopts the combined process of anaerobic fermentation, aerobic denitrification, microalgae production and tail water treatment to treat and recycle the biogas slurry, does not need dilution and liquid discharge, has high recycling utilization rate, low cost, environmental friendliness, almost no sludge generation and emission reduction of CO 2 Oxygen release, no liquid discharge, etc.
(2) The method realizes the biodegradability of biogas slurry through the aerobic denitrification step, has simple and efficient treatment, does not produce secondary pollution, can fully play a role in the microalgae production system through the effluent of the aerobic denitrification bacteria algae sewage front-end treatment system, can simultaneously utilize biogas as a heat source, and can recycle combustion tail gas as CO 2 And the source is used for realizing the efficient production of microalgae.
(3) The collected tail water after microalgae production in the method circularly enters the algae symbiotic treatment equipment for treatment, can ensure stable effluent quality, has low treatment cost and simple operation, and finally realizes no liquid discharge.
(4) The method of the invention carries out repeated circulation treatment and utilization of biogas slurry by utilizing the microalgae production system, and can be matched with a natural evaporation or auxiliary evaporation device of the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system to carry out auxiliary evaporation of excessive biogas slurry, so that the waste of the farm treated by the method of the invention has no liquid discharge, does not need to specially discharge the residual liquid of the waste of the farm, and has the advantages of simple and easy process and no secondary pollution.
(5) The method combines physical, chemical and biological means, realizes the recovery of high added value products of algae biomass while treating the biogas slurry, has simple and feasible process, effectively reduces the running cost of the traditional biogas slurry treatment, and can obtain better benefits; the algae biomass is produced in the form of dry powder, so that the high processing cost of preparing culture solution and the like required by the traditional algae biomass product is avoided.
Drawings
FIG. 1 is a system block diagram of a method for recycling carbon sink oxygen-releasing aquaculture wastewater according to the present invention;
FIG. 2 is a flow chart of a method for recycling carbon sink oxygen-releasing aquaculture wastewater according to the present invention;
FIG. 3 is a schematic diagram of the overall structure of the apparatus for symbiotic treatment of bacteria and algae according to the present invention;
FIG. 4 is an exploded view of the mycorrhizal symbiotic treatment apparatus of the present invention;
wherein, 1-solid-liquid separation equipment, 2-anaerobic fermentation tank, 3-biogas slurry storage tank, 4-algae symbiotic treatment equipment, 41-aerobic denitrifying bacteria algae membrane group, 42-biological filter bed, 43-aeration tank, 44-spray pipe, 5-biogas utilization system, 6-microalgae production equipment, 7-harvesting equipment and 8-drying equipment.
Detailed Description
The present invention will be described in further detail with reference to the following specific embodiments to better embody the advantages of the present invention, and all other embodiments obtained by one skilled in the art without making any inventive effort based on the embodiments of the present invention are within the scope of the present invention. The equipment and reagents used in the present invention are conventional commercially available products in the art, unless specifically indicated.
Example 1
As shown in figures 1 and 2, the method comprises an aerobic denitrifying bacteria algae sewage front-end treatment system for enabling the culture biogas slurry to meet the microalgae culture requirement, and a method for culturing microalgae and fixing CO by utilizing the treated biogas slurry 2 Is composed of two treatment stages of a microalgae production system; the method comprises the following steps:
step 1, a treatment stage of an aerobic denitrifying bacteria algae sewage front-end treatment system
S101, carrying out solid-liquid separation on the manure of the farm by using a solid-liquid separation device 1, regulating the pH value of the separated waste liquid by using acid liquor to reach 7.2, then introducing the waste liquid into an anaerobic fermentation tank 2 for anaerobic fermentation to produce biogas, and then introducing the biogas slurry produced by anaerobic fermentation into a biogas slurry storage tank 3; the acid liquor is HCl solution, and the pH value of the plant excrement is adjusted by adopting the method, so that the plant excrement meets the anaerobic fermentation condition of the anaerobic fermentation tank 2, and the economy is good;
s102, controlling Chemical Oxygen Demand (COD) in the biogas slurry storage tank 3 in the step S101 to be below 2800mg/L, and controlling solid suspended matter concentration (SS) to be below 900 mg/L;
S103, treating the biogas slurry obtained in the step S102 in a bacteria-algae symbiotic treatment device 4 to form a culture solution meeting microalgae culture conditions, wherein the treatment method specifically comprises the following steps:
1) Introducing the biogas slurry obtained in the step S102 into an aeration tank 43 for nitrification treatment, and converting nitrogen-ammonia pollutants in the biogas slurry into non-volatile oxidized nitrate nitrogen through pretreatment nitrification reaction;
2) Inoculating aerobic denitrifying bacteria and microalgae on the aerobic denitrifying bacteria algae film group 41 in advance, distributing the aerated biogas slurry on the aerobic denitrifying bacteria algae film group 41 through a water distribution pump and a spray pipe 44 for aerobic denitrification treatment, and fully degrading and converting ammonia nitrogen in the biogas slurry into N through the aerobic denitrifying bacteria of the aerobic denitrifying bacteria algae film group 41 2 、CO 2 And water, and simultaneously, symbiotic microalgae (mainly chlorella) synchronously absorb CO generated by the aerobic denitrifying bacteria 2 CO in air 2 Oxygen is released, water molecules in the biogas slurry are evaporated to the atmosphere through physical gasification by the aerobic denitrifying bacteria algae membrane group 41, and the breathing action of the aerobic denitrifying bacteria and microalgae can release the water molecules generated by the aerobic denitrifying bacteria and microalgae to the atmosphere;
3) After the pollutants in the biogas slurry are metabolized and utilized by the aerobic denitrifying bacteria and microalgae of the aerobic denitrifying bacteria and microalgae membrane group 41, residual microelements and suspended matters are filtered and adsorbed by the biological filter bed 42, and further metabolized and utilized by the aerobic denitrifying bacteria and microalgae of the biological filter bed 42;
4) Repeatedly circulating the biogas slurry in the step 1) -3) in the algae symbiotic treatment equipment 4, and finally forming a culture solution meeting microalgae culture conditions in the aeration tank 43;
wherein, as shown in fig. 3 and 4, the mycotic symbiotic treatment apparatus 4 comprises: the device comprises an aerobic denitrifying bacteria algae film group 41 for fully degrading biogas slurry, a biological filter bed 42 for adsorbing residual trace elements and suspended matters, an aeration tank 43 for preprocessing and circulating water distribution, and a spray pipe 44 for spraying the biogas slurry on the aerobic denitrifying bacteria algae film group 41, wherein the spray pipe 44 is arranged above the aerobic denitrifying bacteria algae film group 41 and is connected with the aeration tank 43 through a pipeline and a water distribution pump, and the aerobic denitrifying bacteria algae film group 41, the biological filter bed 42 and the aeration tank 43 are sequentially connected in series from top to bottom; through the algae symbiotic treatment equipment 4 and the treatment method, the biogas slurry can be effectively treated to meet the microalgae culture condition, and the biogas slurry does not need to be diluted and discharged without liquid, so that the method has the advantages of high resource utilization rate, low cost, environmental friendliness, almost no sludge generation, no liquid discharge and the like;
s104, introducing the culture solution in the step S103 into microalgae production equipment 6, conveying the biogas in the step S101 to a biogas utilization system 5 through a pipeline, generating power by using the biogas through the biogas utilization system 5, and generating waste heat and tail gas CO 2 Introducing microalgae into microalgae production equipment 6, inoculating microalgae selected from chlorella into the culture solution, and culturing in the microalgae production equipment 6 to obtain an algae solution, wherein the mass concentration of the algae solution is 87g/L; thereby facilitating the solid-liquid separation of the algae mud and tail water by the harvesting equipment 7, having high utilization ratio of resources and no liquid discharge;
wherein the microalgae production equipment 6 is an open type photobioreactor, and a stirring device for keeping the algae liquid in a suspension state is arranged in the open type photobioreactor; the microalgae production equipment 6 can efficiently utilize culture solution, waste heat generated after biogas power generation and tail gas CO 2 Thereby realizing the high-efficiency production of microalgae, and having high utilization rate of resources and CO emission reduction 2 Advantages such as the like;
s105, introducing the algae liquid produced by the microalgae production equipment 6 in the step S104 into a harvesting equipment 7, separating by the harvesting equipment 7 to form algae mud, introducing the algae mud into a drying equipment 8 for drying and pulverizing, and introducing the separated residual tail water into a algae symbiotic treatment equipment 4 again to repeat the step S103;
wherein the harvesting device 7 is a centrifugal device; through above-mentioned harvesting equipment 7, can carry out the retrieval and utilization with the tail water after the microalgae production, the treatment cost is low, and the operation is simple, can realize no liquid emission.
Example 2
The embodiment is basically the same as embodiment 1, and is different from the embodiment in that the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system are both provided with auxiliary evaporators, and part of water molecules in the biogas slurry can be discharged to the atmosphere through the auxiliary evaporators so as to balance the water quantity of the biogas slurry in the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system; thereby maintaining the balance of water quantity in the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system and finally realizing no liquid discharge;
experimental example
The method comprises the steps that the manure of the farm is respectively taken from four pig farms, the test is set to be 4 groups, the manure of the farm is collected and then subjected to solid-liquid separation, sewage after the solid-liquid separation enters an anaerobic fermentation tank 2, after 30 days of anaerobic reaction, biogas slurry at a port is taken out, continuous sampling is carried out for 5 days, 3 samples (repetition) are taken every day, after the biogas slurry is kept stand for 2 days, sediment supernatant is taken, and the physicochemical properties of the biogas slurry sediment supernatant are shown in the following table 1;
TABLE 1 physicochemical Properties of biogas slurry from four groups
The raw biogas slurry with the indexes respectively enters a algae-bacteria symbiotic treatment device 4, and is operated for 24 hours, tail water in an aeration tank 43 is taken for measurement, and the related water quality indexes are shown in table 2;
TABLE 2 physicochemical Properties of biogas slurry from four groups after treatment
From tables 1 and 2, it can be seen that more than 80% of total nitrogen in the raw biogas slurry is converted into nitrite nitrogen and nitrate nitrogen through nitration, and the ammonia nitrogen removal rate in the system is obviously changed; compared with a process of directly diluting biogas slurry, the method has the advantages that the tolerance concentration of microalgae to ammonia nitrogen is about 100mg/L, the inhibition effect of high ammonia nitrogen on the growth of the microalgae is reduced through the nitrification of a dissipative biological film system, and the unit yield of microalgae biomass produced by the treated biogas slurry is higher; meanwhile, after pretreatment, the turbidity of the biogas slurry is obviously reduced, the color is changed from dark black into clear brown, the light permeability of the microalgae production equipment 6 is enhanced, the photosynthesis efficiency is higher, no obvious plankton exists on the surface of the biogas slurry, the concentration of nutrient substances is reduced, and the method is more suitable for the production of microalgae after inoculation;
in the prior art for culturing chlorella by using biogas slurry, the biogas slurry is diluted mainly by adding water or culture solution, chromaticity, turbidity, ammonia nitrogen and the like are used as control variables, the biogas slurry is diluted by adding water, D1 groups are used as test samples, the biogas slurry with consistent chromaticity and turbidity is set and obtained by dilution and is marked as E1, the biogas slurry with consistent ammonia nitrogen and the like is diluted and obtained as E2, and the results are shown in the following table 3;
TABLE 3 physicochemical Properties of biogas slurry after Water dilution
| Group of | PH | Turbidity (NTU) | Chromaticity of | SS | TN | NH 3 -N | NO 3 -N、NO 2 -N | TP | COD |
| E1 | 7.43 | 119±7 | 215.10±17.67 | 64±3 | 297±25 | 32±7 | 327±26 | 14±2 | 103±7 |
| E2 | 8.12 | 198±5 | 523.51±20.44 | 117±53 | 589±19 | 47±3 | 503±34 | 22±3 | 159±2 |
As can be seen from table 3 above, when the chromaticity and turbidity are controlled to be consistent, a large amount of water is required to dilute the biogas slurry, so that the color of the biogas slurry is changed from dark black to clear brown, the light permeability of the microalgae production equipment 6 and the photosynthesis efficiency of the microalgae are ensured, and if the culture solution is added to supplement ammonia nitrogen and the like, the cost of the whole treatment system is increased, and the operation is complicated; when ammonia nitrogen and the like are controlled to be consistent, the water consumption is reduced to a certain extent compared with the former water consumption, but the chromaticity, turbidity and the like are not obviously changed, the color of biogas slurry is changed from deep black to light black, and the light permeability of the microalgae production equipment 6 and the photosynthesis efficiency of microalgae are affected;
therefore, nitrogen in the raw biogas slurry for cultivation is mainly ammonia nitrogen, and even though the nitrogen is diluted, various indexes are still higher, the problems of limited emission and incapacity of being absorbed in the actual treatment process of the biogas slurry need to be solved, otherwise, the water content in a treatment system can be increased by adding multiple purified water for dilution, and the ammonia nitrogen in the biogas slurry is extremely low and is difficult to meet the culture and growth of microalgae, so that the method cannot be realized in practice.
General water quality monitoring indexes of biogas slurry after deep anaerobic treatment (natural state is more than or equal to 30 days) of normal farms, such as Chemical Oxygen Demand (COD) and ammonia Nitrogen (NH) 3 -N) up to 1000-5000 mg.L, respectively -1 And 600-1200 mg.L -1 The biogas slurry generally contains abundant major elements such as nitrogen, phosphorus, potassium and the like, various trace elements such as copper, iron, zinc, calcium and the like, and various substances such as amino acids, vitamins, hydrolytic enzymes, plant hormones, insect disease inhibitors and the like.
The growth of microalgae is affected by various factors such as illumination, temperature, pH, ORP, salinity, conductivity, nutrient components of culture solution and the like, so that the factors need to be strictly optimized when the large-scale microalgae production is carried out.
The high turbidity and high chromaticity of biogas slurry have been proven to be the main factors affecting the growth of chlorella, and the removal of chromaticity and pollutants from biogas slurry by bacteria is becoming a current research hotspot. After the biogas slurry is treated by the dissipative biomembrane system, compared with the original biogas slurry, the turbidity after the treatment is obviously reduced, the chromaticity is changed from muddy black to clear, the photosynthetic reaction efficiency of microalgae can be obviously increased, and the yield is greatly improved. The original method for culturing microalgae by using biogas slurry reduces concentrations of ammonia nitrogen, COD and the like through dilution, but turbidity and chromaticity have no significant change in magnitude, and the new pretreatment method introduces functional bacteria to participate in nitrification and denitrification, so that the chromaticity and turbidity can be greatly reduced, the growth rate of the microalgae is further influenced, and the yield is improved.
The ammonia nitrogen concentration in the biogas slurry is high, so that the growth of microalgae is often inhibited, the tolerance of chlorella Chlorella vulgaris ATCC 13482 to ammonia nitrogen is only 100mg/L, and the tolerance to nitrate nitrogen can reach 350mg/L, so that the nitrification can be used as a pretreatment step to convert ammonia nitrogen into nitrate nitrogen, and the inhibition of ammonia nitrogen to microalgae is reduced; the nitrifying process of the dissipative biomembrane system can realize that ammonia nitrogen in biogas slurry is more than or equal to 70 percent and is converted into nitrate nitrogen, and can obviously improve the absorption of microalgae to nitrogen, thereby improving the growth speed of the microalgae.
Meanwhile, functional aerobic denitrifying bacteria are introduced into the aerobic denitrifying bacteria algae film group 41 in the method of the invention for exploration, and the exploration is as follows:
compared with the traditional technical process for culturing microalgae by using biogas slurry, the novel method introduces aerobic denitrifying bacteria into the treatment process, and during the process of removing and converting functional bacteria to participate in pollutants, symbiotic bacterial communities are naturally formed, and through field verification, a plurality of bacterial communities beneficial to microalgae growth, such as alpha-proteobacteria and bacterioides, are found to be main bacterial communities for treating sewage and promoting microalgae agglomeration in cooperation with the microalgae, and gamma-proteobacteria are commonly found in domesticated sludge of sewage treatment plants, and a plurality of hydrogen nutrition denitrifying bacteria are reported to belong to gamma-proteobacteria;
these results indicate that the phylum Bactoides and the phylum Proteobacteria and the class Gammapoproteobacteria below promote the growth of microalgae. unidentified_cyanobacteria is the most dominant genus of D6, while the dominant genera in DN6 and DCN6 are Cellvibrio and Sphingobacterium. The relative abundance of Flavobacterium, comamonas, microbacterium, dyadobacter and Paenibacillus in DN6 and DCN6 are higher than D6;
cellvibrio is a xylanolytic bacterium and is used in research to promote microalgae growth and propagation in xylan media systems. Sphingobacterium is commonly found in microalgae culture systems and is high in abundance, and growth and propagation of microalgae are likely to be facilitated. Flavobacterium is a genus under the phylum of Bactoides, is a plant growth promoting bacterium, has been found to be capable of effectively improving the biological removal rate of phosphorus in a water bloom system for many times, and has been found to be possibly in close relation with the degradation of algae-derived organic matters. The main genus of Comamonaceae is Comamonas, which is a type of denitrifying phosphorus accumulating bacteria. In addition, the genus Microbacterium has an inhibitory effect on microalgae, and has a promoting effect on microalgae growth. The relative abundance of Microbacterium in the system of the application is higher than that of a microalgae single system, which indicates that the Microbacterium has a promoting effect on the growth of microalgae. Dyadobacter is a bacterium commonly found in microalgae photoreactors, and Paenibacillus is beneficial to biological flocculation and harvesting of chlorella Chlorella vulgaris;
in summary, the main bacterial genera in DN6 and DCN6 are common in all kinds of microalgae photoreactors and have the function of promoting microalgae growth. Compared with DN1 and DCN1, the sum of the relative abundance of dominant bacteria (the relative abundance is more than 1%) in DN6 and DCN6 is increased to 77% and 78%, respectively, and the uniformity of dominant bacteria is high. The higher the uniformity of dominant bacterial colony, the more complex the relationship of the dominant bacterial colony and the dominant bacterial colony, which means that the colony structure in the bacterial algae system DN and DCN is more stable, thereby being beneficial to resisting invasion of other organisms and avoiding collapse of the system. Compared with a microalgae single system, the bacteria community in the microalgae system has low species abundance, high uniformity, more complex interrelation and more stable structure, and dominant bacteria are beneficial to promoting the growth of microalgae.
Claims (6)
1. The method for recycling the carbon-sink oxygen-releasing type culture sewage is characterized by comprising the following steps of:
step 1, a treatment stage of an aerobic denitrifying bacteria algae sewage front-end treatment system
S101, carrying out solid-liquid separation on the manure in the farm by a solid-liquid separation device (1), regulating the pH value of the separated waste liquid to 6.5-7.5, then introducing the waste liquid into an anaerobic fermentation tank (2), carrying out anaerobic fermentation to produce biogas, and then introducing the biogas slurry produced by anaerobic fermentation into a biogas slurry storage tank (3) by taking supernatant;
s102, controlling the chemical oxygen demand in the biogas slurry storage tank (3) in the step S101 to be less than 3000mg/L, and controlling the concentration of solid suspended matters to be less than 1000 mg/L;
s103, enabling the biogas slurry obtained in the step S102 to enter a bacteria-algae symbiotic treatment device (4) for treatment to form a culture solution meeting microalgae culture conditions;
step 2, the treatment stage of the microalgae production system
S104, introducing the culture solution in the step S103 into microalgae production equipment (6), conveying the biogas in the step S101 to a biogas utilization system (5) through a pipeline, generating power by using the biogas through the biogas utilization system (5), and generating waste heat and tail gas CO 2 Introducing into microalgae production equipment (6) for culturing microalgae;
s105, introducing the algae liquid produced by the microalgae production equipment (6) in the step S104 into a harvesting equipment (7), separating the algae liquid by the harvesting equipment (7) to form algae mud, introducing the algae mud into a drying equipment (8) for drying and pulverizing, and introducing the separated residual tail water into a algae symbiotic treatment equipment (4) again to repeat the step S103;
the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system can discharge partial water molecules in the biogas slurry to the atmosphere through natural evaporation or auxiliary evaporators, and are used for balancing the water quantity of the biogas slurry in the aerobic denitrifying bacteria algae sewage front-end treatment system and the microalgae production system;
the mycorrhizal symbiotic treatment device (4) of the step S103 includes: the device comprises an aerobic denitrifying bacteria algae film group (41) for fully degrading biogas slurry, a biological filter bed (42) for adsorbing residual trace elements and organic suspended matters, an aeration tank (43) for preprocessing and circulating water distribution, and a spray pipe (44) for spraying the biogas slurry on the aerobic denitrifying bacteria algae film group (41), wherein the spray pipe (44) is arranged above the aerobic denitrifying bacteria algae film group (41) and is connected with the aeration tank (43) through a pipeline and a water distribution pump, and the aerobic denitrifying bacteria algae film group (41), the biological filter bed (42) and the aeration tank (43) are sequentially connected in series from top to bottom;
wherein, the method of step S103 comprises the following steps:
1) Introducing the biogas slurry obtained in the step S102 into an aeration tank (43) for nitrification treatment, and converting nitrogen-ammonia pollutants in the biogas slurry into non-volatile oxidation-state nitrate nitrogen through pretreatment nitrification reaction;
2) Inoculating aerobic denitrifying bacteria and microalgae on an aerobic denitrifying bacteria algae membrane group (41) in advance, uniformly spraying the aerated biogas slurry on the aerobic denitrifying bacteria algae membrane group (41) through a water distribution pump and a spray pipe (44) for aerobic denitrification treatment, and fully degrading and converting the biogas slurry into N through the aerobic denitrifying bacteria and microalgae of the aerobic denitrifying bacteria algae membrane group (41) 2 、CO 2 And water, meanwhile, water molecules in the biogas slurry are evaporated to the atmosphere through physical gasification by an aerobic denitrifying bacteria algae membrane group (41), and the breathing action of the aerobic denitrifying bacteria and microalgae can release water molecules in the biogas slurry to the atmosphere;
3) After partial nitrate nitrogen, phosphorus and potassium in the biogas slurry are metabolized and utilized by aerobic denitrifying bacteria and microalgae of an aerobic denitrifying bacteria algae film group (41), partial microelements and suspended matters are filtered and adsorbed by a biological filter bed (42), and further metabolized and utilized by the aerobic denitrifying bacteria and microalgae of the biological filter bed (42), and the residual biogas slurry after treatment enters an aeration tank (43) again;
4) And (3) repeatedly circulating the biogas slurry in the algae-bacteria symbiotic treatment equipment (4), and finally forming the culture solution meeting the microalgae culture conditions in the aeration tank (43).
2. The method for recycling carbon-sink oxygen-releasing aquaculture wastewater according to claim 1, wherein in the step S101, the pH of the separated wastewater is adjusted to 6.5-7.5 by using acid solution, wherein the acid solution is HCl solution or H 2 SO 4 Any one of the solutions.
3. The method for recycling carbon-sink oxygen-releasing culture sewage according to claim 1, wherein the microalgae production equipment (6) in the step S104 is an open type photobioreactor or a closed type photobioreactor, and a stirring device and/or an aeration device for keeping algae liquid in a suspended state are arranged in the open type photobioreactor or the closed type photobioreactor.
4. The method for recycling carbon-sink oxygen-releasing culture sewage according to claim 1, wherein the microalgae is at least one species selected from the group consisting of chlorella, spirulina, scenedesmus and chlamydomonas, the species is inoculated into the culture solution, and the culture solution is produced in a microalgae production device (6).
5. The method for recycling carbon-sink oxygen-releasing culture sewage according to claim 1, wherein the recovery device (7) in the step S105 is any one of a precipitation device, a filtration device and a centrifugation device.
6. The method for recycling carbon-sink oxygen-releasing culture sewage according to claim 1, wherein the mass concentration of the algae liquid produced by the microalgae production equipment (6) in the step S104 is 0.8-1.5 g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210153653.8A CN114605030B (en) | 2022-02-19 | 2022-02-19 | Method for recycling carbon-sink oxygen-release type cultivation sewage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210153653.8A CN114605030B (en) | 2022-02-19 | 2022-02-19 | Method for recycling carbon-sink oxygen-release type cultivation sewage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114605030A CN114605030A (en) | 2022-06-10 |
| CN114605030B true CN114605030B (en) | 2023-05-23 |
Family
ID=81859259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210153653.8A Active CN114605030B (en) | 2022-02-19 | 2022-02-19 | Method for recycling carbon-sink oxygen-release type cultivation sewage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114605030B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115072878A (en) * | 2022-06-28 | 2022-09-20 | 青岛理工大学 | Low-energy-consumption multi-layer bacteria-algae symbiotic sewage treatment device capable of realizing carbon emission reduction |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103910434B (en) * | 2014-04-25 | 2015-09-02 | 厦门大学 | To exceed standard the membrane type bioreactor of nitrogen phosphorus and treatment process thereof for the treatment of natural pond liquid |
| CN108034624B (en) * | 2018-02-05 | 2021-04-27 | 厦门理工学院 | Biological agent for treating high-concentration ammonia nitrogen wastewater and preparation method thereof |
| CN112723550A (en) * | 2020-12-30 | 2021-04-30 | 湖州弘鑫生态农业科技有限公司 | Be used for aquaculture waste water fungus algae symbiosis bobble biological reaction device |
| CN214571032U (en) * | 2021-03-01 | 2021-11-02 | 江西禾一生物科技有限公司 | Organic wastewater treatment system |
-
2022
- 2022-02-19 CN CN202210153653.8A patent/CN114605030B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN114605030A (en) | 2022-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Microalgae-based wastewater treatment for nutrients recovery: A review | |
| Lu et al. | Critical processes and variables in microalgae biomass production coupled with bioremediation of nutrients and CO2 from livestock farms: A review | |
| US6416993B1 (en) | Method for treating a waste stream using photosynthetic microorganisms | |
| Gu et al. | Enhancement of nutrients removal and biomass accumulation of Chlorella vulgaris in pig manure anaerobic digestate effluent by the pretreatment of indigenous bacteria | |
| US20120214198A1 (en) | Algaculture method | |
| US20030211594A1 (en) | Microalgae for remediation of waste and method of culturing the same | |
| CN106396112A (en) | Composite system used for purifying high ammonia nitrogen pig breeding biogas slurry via algae-bacterium symbiosis/ecological floating bed combined technology | |
| CN106430820B (en) | A kind of biological treatment device and its technique of high ammonia nitrogen pig raising biogas slurry | |
| CN104150684A (en) | Aquaculture wastewater treatment process | |
| WO2010003255A1 (en) | Process for continual multiphase recycling of solid and liquid organic waste for a greenhouse culture | |
| Shayesteh et al. | Can CO2 addition improve the tertiary treatment of anaerobically digested abattoir effluent (ADAE) by Scenedesmus sp.(Chlorophyta)? | |
| CN110885756A (en) | Method for culturing oil-producing chlorella by using sludge anaerobic digestive fluid | |
| CN105417877A (en) | Novel livestock wastewater treatment process | |
| CN104628225A (en) | Treatment method of ammonia-nitrogen-containing industrial wastewater | |
| CN103663715A (en) | Biotreatment method for efficiently purifying biogas slurry with microalgae | |
| CN111170571B (en) | Liquid manure treatment process for livestock manure | |
| CN109942091B (en) | Bacterial-algae attached biological fiber bed, preparation method thereof and method for strengthening treatment of pig wastewater for nitrogen and phosphorus removal | |
| CN101701197B (en) | Novel microorganism flora mixture and mixed nutrient medium thereof | |
| CN114605030B (en) | Method for recycling carbon-sink oxygen-release type cultivation sewage | |
| CN109081447B (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas | |
| CN108342338B (en) | Method for treating pharmaceutical wastewater containing antibiotics | |
| CN113354100A (en) | Method for recycling nitrogen and phosphorus resources and neutralizing carbon by coupling filamentous algae after moderately nitrifying high-concentration ammonia nitrogen wastewater | |
| CN219752089U (en) | Resource continuous treatment system for purifying biogas slurry based on energy chlorella | |
| CN109502914B (en) | Sewage treatment system for treating biogas slurry by using chlorella | |
| US20210380452A1 (en) | Method for treatment and resource utilization of acidic organic wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220808 Address after: Room 401-1, Building 01, No. 60, Xiankun Road, Jianye District, Nanjing City, Jiangsu Province, 210029 Applicant after: Nanjing Weilei Ecological Technology Co., Ltd. Address before: No. 1154, Nanjing National Agricultural Innovation Park scientific innovation center, No. 8, Xingzhi Road, Pukou District, Nanjing, Jiangsu 210031 Applicant before: Jiangsu zaohui Biotechnology Research Institute Co.,Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |