CN114958592A - Anti-tumor immune cell culture device - Google Patents
Anti-tumor immune cell culture device Download PDFInfo
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- CN114958592A CN114958592A CN202210671557.2A CN202210671557A CN114958592A CN 114958592 A CN114958592 A CN 114958592A CN 202210671557 A CN202210671557 A CN 202210671557A CN 114958592 A CN114958592 A CN 114958592A
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- 210000002865 immune cell Anatomy 0.000 title claims abstract description 29
- 230000000259 anti-tumor effect Effects 0.000 title claims abstract description 25
- 238000004113 cell culture Methods 0.000 title claims abstract description 18
- 210000004027 cell Anatomy 0.000 claims abstract description 29
- 238000005485 electric heating Methods 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 2
- 206010028980 Neoplasm Diseases 0.000 description 15
- 238000002649 immunization Methods 0.000 description 6
- 230000003053 immunization Effects 0.000 description 6
- 210000003171 tumor-infiltrating lymphocyte Anatomy 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 206010034960 Photophobia Diseases 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000009169 immunotherapy Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/54—Constructional details, e.g. recesses, hinges hand portable
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention discloses an anti-tumor immune cell culture device which comprises a temperature control structure, a cell shaking structure and a light-shading box cover structure, wherein the cell shaking structure is arranged on the top surface of the temperature control structure. According to the invention, the output shaft of the servo motor is utilized to rotate the rotating shaft through the coupler, the flat plate is driven by the output shaft of the servo motor to rotate forward for a half circle and then rotate backward for a half circle under the traction of the rotating shaft, so that the test tube in the rubber soft tank is vibrated, and when the temperature is required to be controlled, the vacuum generator on the side wall of the fixed box body is started, so that the air in the sealed box is pumped away by the output end of the vacuum generator to form a negative pressure state.
Description
Technical Field
The invention relates to the field of immune cell culture equipment, in particular to an anti-tumor immune cell culture device.
Background
Tumor Infiltrating Lymphocytes (TILs) are Lymphocytes that leave blood and enter tumors, and are a large important component of the Tumor microenvironment, and in recent decades, TILs have gained more and more attention because scientists find that these cells can recognize a plurality of nonspecific cancer cells, so that analysis of genetic markers and related proteins of TILs provides clues for us to understand the progress of cancer diseases, and has important significance for Tumor prognosis or prediction.
The tumor immunotherapy has great potential shown in the clinical tumor treatment process, so that the tumor immunotherapy becomes a posterous show in the tumor treatment research field, and has been gradually developed into a fourth treatment method after surgery, radiotherapy and chemotherapy, in the tumor immunization research process, a tumor immunization research model is constructed in vitro to verify the effectiveness of a tumor immunization research strategy, and the intrinsic mechanism of the tumor immunization research model is explored, so that the tumor immunization research model is an indispensable research link, in a plurality of tumor immunization research models, the most widely applied in vitro research model is an immune cell and tumor cell co-culture model, temperature control and photophobia for promoting cell propagation in the immune cell culture process are indispensable in the culture link, and simultaneously, after the cells are sampled, the cells are manually put into a test tube to be vibrated to replace manual vibration, and the temperature and photophobia control are carried out on cell culture, an anti-tumor immune cell culture device is proposed.
Disclosure of Invention
The invention aims to provide an anti-tumor immune cell culture device to solve the defects in the technology.
In order to achieve the above purpose, the invention provides the following technical scheme: an anti-tumor immune cell culture device comprises a temperature control structure, a cell shaking structure and a light-shading box cover structure, wherein the top surface of the temperature control structure is provided with the cell shaking structure, the top surface of the cell shaking structure is hinged with the light-shading box cover structure, the cell shaking structure is positioned between the temperature control structure and the light-shading box cover structure, the temperature control structure comprises a fixed box body, box legs, an electric heating plate, a seal box and a vacuum generator, the peripheral side wall of the inner cavity of the fixed box body is fixedly connected with the periphery of the outer side of the seal box, the inner wall of the bottom of the fixed box body is fixedly connected with the bottom surface of the electric heating plate through screws, the vacuum generator is fixedly arranged on the side wall of the fixed box body, and the output end of the vacuum generator penetrates into the inner cavity of the seal box;
the cell shaking-up structure comprises an incubator, a rotating shaft, a rubber soft tank, a flat plate, a spring, a hoop, an adjusting part, a motor bin, a servo motor, a bearing and a coupler, wherein the inner wall of the back of the incubator is rotatably connected with one end of the rotating shaft, the interior of the flat plate is fixed on the outer ring of the rotating shaft, one end of the spring is positioned at four corners of the bottom of the flat plate, the other end of the spring is fixedly connected with the inner wall of the bottom of the incubator, one end of the rotating shaft penetrating through the flat plate is fixedly connected with an output shaft of the servo motor through the coupler, the mounting surface of the servo motor is fixedly mounted on the inner wall of the motor bin, and the back of the motor bin is fixedly connected with the side wall of the incubator;
the light-resistant case lid structure is including transparent case lid, the handle, the fixed plate, the fixed block, a slide rail, the slider, window shade and fly leaf, wherein, the welding of top back right side of transparent case lid has two fixed blocks, one side that the fixed block is close to each other respectively with the both ends fixed connection of fixed plate, and the lateral wall of fixed plate and one side fixed connection of window shade, the opposite side of window shade and the lateral wall fixed connection of fly leaf, and the both ends of fly leaf respectively with the one side fixed connection that the slider is close to each other, and the slider is at the inside sliding connection of slide rail, the bottom fixed mounting of slide rail is on the top surface of transparent case lid.
As a preferable scheme of the invention, four corners of the bottom surface of the fixed box body are fixedly connected with the box legs through screws, and the vacuum generator and the electric heating plate are fixedly connected with an external power supply through leads.
As a preferred scheme of the invention, the top surface of the flat plate is provided with four through holes for placing the rubber soft tank, the anchor ear is arranged at the top of the outer ring of the rubber soft tank, and two ends of the anchor ear are sleeved with the adjusting device.
As a preferable scheme of the invention, a round hole is formed in the side wall of the front face of the incubator, the bearing is arranged in the round hole, and the inner ring of the bearing is movably connected with the outer ring of the output shaft of the servo motor.
As a preferable scheme of the present invention, a power input end of the servo motor is fixedly connected to an external power source through a wire.
As a preferable aspect of the present invention, the top sidewall of the movable plate is fixedly connected to the bottom surface of the handle.
In a preferred embodiment of the present invention, the vertical section of the window shade is a continuous "W" shape, and the window shade is made of a window shade net or a window shade cloth having a window shade performance.
In the technical scheme, the invention provides the following technical effects and advantages:
according to the invention, the output shaft of the servo motor is utilized to rotate the rotating shaft through the coupler, the flat plate is driven by the output shaft of the servo motor to rotate forward for a half circle and then rotate backward for a half circle under the traction of the rotating shaft, so that the test tube in the rubber soft tank is vibrated, and when the temperature is required to be controlled, the vacuum generator on the side wall of the fixed box body is started, so that the air in the sealed box is pumped away by the output end of the vacuum generator to form a negative pressure state.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.
FIG. 1 is a schematic perspective view of an anti-tumor immune cell culture apparatus according to the present invention;
FIG. 2 is a schematic perspective side view of an anti-tumor immune cell culture apparatus according to the present invention;
FIG. 3 is a schematic cross-sectional structural view of a cell shaking structure of an anti-tumor immune cell culture device according to the present invention;
FIG. 4 is a schematic perspective view of a light-shielding cover of the anti-tumor immunocyte culturing device according to the present invention;
FIG. 5 is a schematic cross-sectional view of a temperature control structure of an anti-tumor immune cell culture apparatus according to the present invention;
FIG. 6 is a schematic enlarged view of part A of FIG. 3 of an apparatus for culturing anti-tumor immune cells according to the present invention;
FIG. 7 is a schematic view of a part A of the enlarged structure of the anti-tumor immune cell culture apparatus of FIG. 4 according to the present invention.
Description of reference numerals:
1. a temperature control structure; 11. fixing the box body; 12. box legs; 13. an electrical heating plate; 14. a sealing box; 15. a vacuum generator; 2. shaking up the cells; 21. an incubator; 22. a rotating shaft; 23. a rubber soft tank; 24. a flat plate; 25. a spring; 26. hooping; 27. adjusting; 28. a motor compartment; 29. a servo motor; 210. a bearing; 211. a coupling; 3. a light-shading box cover structure; 31. a transparent case cover; 32. a handle; 33. a fixing plate; 34. a fixed block; 35. a slide rail; 36. a slider; 37. a window blind; 38. a movable plate.
Detailed Description
In order to make the technical solution and implementation of the present invention more clearly explained and illustrated, several preferred embodiments for implementing the technical solution of the present invention are described below.
The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, or uses. It should be understood that throughout the drawings, identical or similar reference numerals indicate identical or similar parts and features. The drawings are only schematic representations of the concepts and principles of the embodiments of the disclosure, and do not necessarily show specific dimensions or proportions of the various embodiments of the disclosure. While certain features of the present disclosure may be shown in exaggerated form in certain drawings to illustrate relevant details or structures of embodiments of the disclosure, the various publications, patents, and published patent specifications cited herein, the disclosures of which are hereby incorporated by reference in their entirety, will now be described in detail, in connection with the embodiments of the disclosure which are to be considered as illustrative and not restrictive, it being understood that the illustrated embodiments are merely some of the embodiments of the disclosure.
In the description of the present invention, unless explicitly stated or limited otherwise, the terms "first", "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; the term "plurality" means two or more unless specified or indicated otherwise; the terms "connected" and "fixed" are used broadly, and for example, "connected" may be a fixed connection or a detachable connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.
In the description of the present application, it should be understood that the terms "upper", "lower", "left", "right", and the like used in the description of the embodiments of the present application are used in the angle shown in the drawings, and should not be construed as limiting the embodiments of the present application. In addition, in this context, it will also be understood that when an element is referred to as being "on" or "under" another element, it can be directly on "or" under "the other element or be indirectly on" or "under" the other element via an intermediate element.
Example one
Referring to the attached figures 1 to 7 of the specification, an anti-tumor immune cell culture device:
the device comprises a temperature control structure 1, a cell shaking structure 2 and a light-proof box cover structure 3, wherein the top surface of the temperature control structure 1 is provided with the cell shaking structure 2, the top surface of the cell shaking structure 2 is hinged with the light-proof box cover structure 3, the cell shaking structure 2 is positioned between the temperature control structure 1 and the light-proof box cover structure 3, the temperature control structure 1 comprises a fixed box body 11, box legs 12, an electric heating plate 13, a sealing box 14 and a vacuum generator 15, wherein the peripheral side wall of an inner cavity of the fixed box body 11 is fixedly connected with the periphery of the outer side of the sealing box 14, the inner wall of the bottom of the fixed box body 11 is fixedly connected with the surface of the bottom of the electric heating plate 13 through screws, the vacuum generator 15 is fixedly arranged on the side wall of the fixed box body 11, when the temperature needs to be controlled, the output end of the vacuum generator 15 pumps away the air in the sealing box 14 to form a negative pressure state by starting the vacuum generator 15 on the side wall of the fixed box body 11, the principle that heat in a vacuum environment is difficult to transfer is utilized, so that the heat transfer of the electric heating plate 13 is blocked, the purpose of temperature control is achieved, and the output end of the vacuum generator 15 penetrates into the inner cavity of the seal box 14;
the cell shaking structure 2 comprises an incubator 21, a rotating shaft 22, a rubber soft tank 23, a flat plate 24, a spring 25, a hoop 26, an adjusting 27, a motor cabin 28, a servo motor 29, a bearing 210 and a coupler 211, wherein the inner wall of the back of the incubator 21 is rotatably connected with one end of the rotating shaft 22, the inner part of the flat plate 24 is fixed on the outer ring of the rotating shaft 22, one end of the spring 25 is positioned at four corners of the bottom of the flat plate 24, the other end of the spring is fixedly connected with the inner wall of the bottom of the incubator 21, a culture test tube containing immune cells is placed in the inner cavity of the rubber soft tank 23, then the opening of the rubber soft tank 23 is tightened by the adjusting 27 and utilizing the softness of the rubber soft tank 23, so that the test tube is fixed by the rubber soft tank 23, one end of the rotating shaft 22 penetrating through the flat plate 24 is fixedly connected with the output shaft of the servo motor 29 through the coupler 211, and the mounting surface of the servo motor 29 is fixedly mounted on the inner wall of the motor cabin 28, the back of the motor bin 28 is fixedly connected with the side wall of the incubator 21, under the condition that the servo motor 29 is set to rotate forward for a half circle and then rotate backward for a half circle, the servo motor 29 in the inner cavity of the motor bin 28 is started, the output shaft of the servo motor 29 is enabled to rotate the rotating shaft 22 through the coupler 211, the flat plate 24 is enabled to do the movement of rotating forward for a half circle and then rotating backward for a half circle along with the output shaft of the servo motor 29 under the traction of the rotating shaft 22, and therefore the test tube in the rubber soft tank 23 is enabled to be vibrated.
Example two
Based on the first embodiment, referring to the attached drawings 1 to 7 of the specification, an anti-tumor immune cell culture device is provided:
the light-proof box cover structure 3 comprises a transparent box cover 31, a handle 32, a fixed plate 33, fixed blocks 34, a slide rail 35, a slide block 36, a light-shading curtain 37 and a movable plate 38, wherein the two fixed blocks 34 are welded on the right side of the back of the top of the transparent box cover 31, the mutually close sides of the fixed blocks 34 are respectively fixedly connected with the two ends of the fixed plate 33, the side wall of the fixed plate 33 is fixedly connected with one side of the light-shading curtain 37, the other side of the light-shading curtain 37 is fixedly connected with the side wall of the movable plate 38, when the cells in the rubber soft tank 23 need or do not need illumination, the handle 32 on the transparent box cover 31 is pulled, the movable plate 38 drives the light-shading curtain 37 to stretch or fold under the traction of the handle 32, the two ends of the movable plate 38 are respectively fixedly connected with the mutually close sides of the slide block 36, the slide block 36 is slidably connected in the slide rail 35, the bottom of the slide rail 35 is fixedly installed on the top surface of the transparent box cover 31, the bottom surface four corners of fixed box 11 all passes through screw fixed connection with case leg 12, and vacuum generator 15 and electric heating board 13 all pass through wire and external power supply fixed connection, four through-holes that supply rubber soft jar 23 to place are seted up to dull and stereotyped 24's top surface, and staple bolt 26 sets up the outer lane top at rubber soft jar 23, the both ends cover of staple bolt 26 is equipped with regulation 27, the round hole has been seted up on the positive lateral wall of incubator 21, bearing 210 sets up the inside at the round hole, and bearing 210 inner circle and servo motor 29's output shaft outer lane swing joint, servo motor 29's power input end passes through electric wire and external power supply fixed connection, the top lateral wall of fly leaf 38 and the bottom fixed surface of handle 32 are connected, the perpendicular cross-section of window shade 37 is continuous "W" form, and window shade 37 adopts the window screen or the shading cloth that have shading performance to make.
Referring to the attached drawings 1 to 7 in the specification, the working principle of the invention is as follows:
firstly, placing a culture test tube containing immune cells into an inner cavity of a rubber soft tank 23, then tightly tightening an opening of the rubber soft tank 23 by adjusting 27 and utilizing the softness of the rubber soft tank 23, so that the rubber soft tank 23 fixes the test tube, then covering a light-shielding box cover structure 3 to seal the opening at the top of a cell shaking structure 2, under the condition that a servo motor 29 is set to rotate forwards for a half circle and then rotate backwards for a half circle, then starting a servo motor 29 in an inner cavity of a motor bin 28, enabling an output shaft of the servo motor 29 to rotate a rotating shaft 22 through a coupler 211, enabling a flat plate 24 to do actions of rotating forwards for a half circle and then rotating backwards for a half circle along with the output shaft of the servo motor 29 under the traction of the rotating shaft 22, and further enabling the test tube in the rubber soft tank 23 to be vibrated;
then, a power supply of the electric heating plate 13 is switched on, the electric heating plate 13 generates heat, the heat is transmitted into the cell shaking structure 2 through the sealing box 14, finally the heat is transmitted into a test tube in the rubber soft tank 23 to provide the temperature required by culture for the cells in the test tube, when the temperature needs to be controlled, the output end of the vacuum generator 15 is started to pump away the air in the sealing box 14 to form a negative pressure state by the aid of the vacuum generator 15 on the side wall of the fixed box body 11, and the heat transmission of the electric heating plate 13 is blocked by the aid of the principle that the heat in a vacuum environment is difficult to transmit, so that the temperature is controlled;
when the cells in the rubber soft tank 23 need or do not need illumination, the handle 32 on the transparent tank cover 31 is pulled, the movable plate 38 carries the window shade 37 to stretch or fold under the traction of the handle 32, and the movable plate 38 slides in the slide rail 35 by virtue of the slide block 36, so that the stretching and folding actions of the window shade 37 are easier.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.
Claims (9)
1. The utility model provides an anti tumour immune cell culture apparatus, includes accuse temperature structure (1), cell shake even structure (2) and light-resistant case lid structure (3), its characterized in that: the top surface of the temperature control structure (1) is provided with a cell shaking structure (2), the top surface of the cell shaking structure (2) is hinged with a light-proof box cover structure (3), the cell shaking structure (2) is positioned between the temperature control structure (1) and the light-proof box cover structure (3), and the temperature control structure (1) comprises a fixed box body (11), box legs (12), an electric heating plate (13), a sealing box (14) and a vacuum generator (15), wherein, the peripheral side walls of the inner cavity of the fixed box body (11) are fixedly connected with the periphery of the outer side of the seal box (14), the inner wall of the bottom of the fixed box body (11) is fixedly connected with the bottom surface of the electric heating plate (13) through a screw, the vacuum generator (15) is fixedly arranged on the side wall of the fixed box body (11), and the output end of the vacuum generator (15) penetrates into the inner cavity of the seal box (14).
2. The device for culturing anti-tumor immune cells of claim 1, wherein: the cell shaking-up structure (2) comprises an incubator (21), a rotating shaft (22), a rubber soft tank (23), a flat plate (24), a spring (25), a hoop (26), an adjusting device (27), a motor cabin (28), a servo motor (29), a bearing (210) and a coupler (211), wherein the inner wall of the back of the incubator (21) is rotatably connected with one end of the rotating shaft (22), the inner part of the flat plate (24) is fixed on the outer ring of the rotating shaft (22), one end of the spring (25) is positioned at four corners of the bottom of the flat plate (24), the other end of the spring is fixedly connected with the inner wall of the bottom of the incubator (21), the rubber soft tank (23) is arranged on the top surface of the flat plate (24), one end of the rotating shaft (22) penetrating through the flat plate (24) is fixedly connected with an output shaft of the servo motor (29) through the coupler (211), and the mounting surface of the servo motor (29) is fixedly mounted on the inner wall of the motor cabin (28), the back of the motor bin (28) is fixedly connected with the side wall of the incubator (21).
3. The device for culturing the anti-tumor immune cells according to claim 1, wherein: light-resistant case lid structure (3) is including transparent case lid (31), handle (32), fixed plate (33), fixed block (34), slide rail (35), slider (36), window shade (37) and fly leaf (38), wherein, the welding of the top back right side of transparent case lid (31) has two fixed blocks (34), one side that fixed block (34) are close to each other respectively with the both ends fixed connection of fixed plate (33), and the lateral wall of fixed plate (33) and one side fixed connection of window shade (37), the opposite side of window shade (37) and the lateral wall fixed connection of fly leaf (38), and the both ends of fly leaf (38) respectively with the one side fixed connection that slider (36) are close to each other, and slider (36) are at the inside sliding connection of slide rail (35), the bottom fixed mounting of slide rail (35) is on the top surface of transparent case lid (31).
4. The device for culturing the anti-tumor immune cells according to claim 1, wherein: four corners of the surface of the bottom of the fixed box body (11) are fixedly connected with the box legs (12) through screws, and the vacuum generator (15) and the electric heating plate (13) are fixedly connected with an external power supply through wires.
5. The device for culturing the anti-tumor immune cells according to claim/2, wherein: four through-holes that supply rubber flexible tank (23) to place are seted up on the top surface of flat board (24), and staple bolt (26) set up at the outer lane top of rubber flexible tank (23), and the both ends cover of staple bolt (26) is equipped with regulation (27).
6. The device for culturing antitumor immune cells according to claim 2, wherein: a round hole is formed in the side wall of the front face of the incubator (21), the bearing (210) is arranged inside the round hole, and the inner ring of the bearing (210) is movably connected with the outer ring of the output shaft of the servo motor (29).
7. The device for culturing antitumor immune cells according to claim 2, wherein: and the power input end of the servo motor (29) is fixedly connected with an external power supply through a wire.
8. The device for culturing the anti-tumor immune cells according to claim 3, wherein: the top side wall of the movable plate (38) is fixedly connected with the bottom surface of the handle (32).
9. The device for culturing the anti-tumor immune cells according to claim 3, wherein: the vertical section of the window shade (37) is in a continuous W shape, and the window shade (37) is made of a window shade net or a window shade cloth with a window shade performance.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210671557.2A CN114958592A (en) | 2022-06-14 | 2022-06-14 | Anti-tumor immune cell culture device |
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| CN202210671557.2A CN114958592A (en) | 2022-06-14 | 2022-06-14 | Anti-tumor immune cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117804056A (en) * | 2024-02-29 | 2024-04-02 | 成都千麦医学检验所有限公司 | Temperature-changing incubator for enzyme-linked immunosorbent assay |
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