CN214458056U - Cell culture device for bioreactor - Google Patents

Cell culture device for bioreactor Download PDF

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Publication number
CN214458056U
CN214458056U CN202022654855.XU CN202022654855U CN214458056U CN 214458056 U CN214458056 U CN 214458056U CN 202022654855 U CN202022654855 U CN 202022654855U CN 214458056 U CN214458056 U CN 214458056U
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China
Prior art keywords
motor
cell culture
rotating shaft
shell
fixing
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CN202022654855.XU
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Chinese (zh)
Inventor
吴伊光
李芬
张立平
魏燕午
薛峰
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Zhenjiang Jianggong Bioengineering Complete Equipment Co ltd
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Zhenjiang Jianggong Bioengineering Complete Equipment Co ltd
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Abstract

The utility model discloses a cell culture device for bioreactor, including device shell, sealed pad and observation window, the inside bottom of device shell is provided with the motor, the surface mounting of rotation axis has the fixed bolster, the standing groove has been seted up on the surface of fixed bolster, the internally mounted of standing groove has the pinch-off blades, the end-to-end connection of pinch-off blades has fixing spring, and fixing spring is connected with the lateral wall of standing groove, the top of rotation axis is provided with lighting device, the closed door is installed in the front of device shell, the closed door is provided with sealed the pad with the position of the department of meeting of device shell, the observation window has been seted up on the surface of closed door. This a cell culture device for bioreactor is provided with lighting device, and lighting device is inside to be provided with the three-layer light, and the position of three-layer light can be adjusted through the handle to the three-layer light, has more to need to change the inside illumination intensity of device.

Description

Cell culture device for bioreactor
Technical Field
The utility model relates to a cell culture technical field specifically is a cell culture device for bioreactor.
Background
Cell culture refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain main structures and functions, and is also called cell cloning technology, the formal term in biology is cell culture technology, and cell culture is an essential process for the whole bioengineering technology and one of the biological cloning technologies, and the cell culture is the large-scale cloning of cells, and the cell culture technology is an important and common technology in the research method of cell biology.
Cell culture is a commonly used technical means in the aspects of biological medicine and medical treatment at present, and in the present cell culture process, because different cells are different in optimal temperature, light intensity and the like during culture, equipment is required to assist in the culture process so as to realize the adjustment of the temperature and the light intensity, so a cell culture device for a bioreactor is provided so as to solve the problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell culture device for bioreactor to it needs equipment to assist on the existing market that the above-mentioned background art provided to solve, with the problem of realization to its temperature and the regulation of light intensity.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cell culture device for bioreactor, includes device shell, sealing door, sealed pad and observation window, the inside bottom of device shell is provided with the motor, the both sides position of motor is provided with the air heater, the top of air heater is fixed with the fan, the upside of motor is connected with the rotation axis, the surface mounting of rotation axis has the fixed bolster, the standing groove has been seted up on the surface of fixed bolster, the internally mounted of standing groove has the pinch-off blades, the end-to-end connection of pinch-off blades has fixing spring, and fixing spring is connected with the lateral wall of standing groove, the top of rotation axis is provided with lighting device, the sealing door is installed in the front of device shell, the position of department of meeting of sealing door and device shell is provided with sealed pad, the observation window has been seted up on the surface of sealing door.
The shape and the size of the device main body are the same as those of the inside of the device shell, the thickness of the illuminating lamp and the distance between the illuminating lamp and the sliding grooves are equal, and the width of each sliding groove is 2-5 cm.
Preferably, the motor is installed at the center of the ground inside the device shell, two ends of the motor are respectively and fixedly connected with the device shell and the rotating shaft, the air heaters are symmetrically arranged relative to the motor, and the air heaters are in contact connection with the motor.
Preferably, the number of the fixing supports is 8, the fixing supports are uniformly distributed on the surface of the rotating shaft, the fixing supports are staggered in a crossed mode and do not interfere with each other, and the height of the rotating shaft is smaller than the distance between the motor and the lighting device.
Preferably, 2 placing grooves are formed in the upper surface of the fixing support, the clamping plate forms a left-right moving structure in the placing grooves through fixing springs, and the height of the clamping plate is larger than the depth of the placing grooves.
Preferably, lighting device includes device main part, spout, light, stopper and handle, install the spout on the inside lateral wall of device main part, spout internally mounted has the light, be provided with the stopper between the spout, the end-to-end connection of the light of the below of device main part and below top has the handle.
Preferably, the shape and size of the device main body are the same as the shape and size of the inside of the device shell, the thickness of the illuminating lamp and the distance between the sliding grooves are equal, the width of the sliding grooves is 2-5cm, the width of the limiting blocks is equal to the width of the sliding grooves, and the handle is fixedly connected with the illuminating lamp.
Compared with the prior art, the beneficial effects of the utility model are that: this a cell culture device for bioreactor:
(1) the three-layer illuminating lamp is arranged in the illuminating device, and the position of the three-layer illuminating lamp can be adjusted through the handle, so that the illumination intensity in the illuminating device is required to be changed.
(2) The culture dish is provided with a rotating shaft, a fixed support is arranged on the rotating shaft, the culture dish is placed on the fixed support, and the rotating shaft rotates slowly, so that nutrient solution in the culture dish cannot stand still under the centrifugal effect, and cells in the culture dish are effectively protected;
(3) the placing groove is formed in the fixing support, the clamping plate and the fixing spring are arranged in the placing groove, the position can be adjusted according to the size of the culture dish, the air heater is arranged at the bottom of the device, and the fan is arranged on the air heater and can accelerate the flow of hot air.
Drawings
FIG. 1 is a schematic view of the main sectional structure of the present invention;
FIG. 2 is a schematic side sectional view of the present invention;
fig. 3 is a schematic top view of the lighting device of the present invention;
FIG. 4 is a schematic view of a top-down structure of the rotating shaft of the present invention;
FIG. 5 is a schematic view of the structure at A in FIG. 1 according to the present invention;
fig. 6 is a schematic structural diagram of the point B in fig. 1 according to the present invention.
In the figure: 1. a device housing; 2. an electric motor; 3. a hot air blower; 4. a fan; 5. a rotating shaft; 6. fixing a bracket; 7. a placement groove; 8. a clamping plate; 9. fixing the spring; 10. an illumination device; 101. a device main body; 102. a chute; 103. an illuminating lamp; 104. a limiting block; 105. a handle; 11. a closing door; 12. A gasket; 13. and (4) an observation window.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-6, the present invention provides a technical solution: a cell culture device for a bioreactor comprises a device shell 1, a motor 2, an air heater 3, a fan 4, a rotating shaft 5, a fixing support 6, a placing groove 7, a clamping plate 8, a fixing spring 9, a lighting device 10, a closed door 11, a sealing gasket 12 and an observation window 13, wherein the motor 2 is arranged at the bottom end inside the device shell 1, the air heater 3 is arranged at the positions on two sides of the motor 2, the fan 4 is fixed above the air heater 3, the rotating shaft 5 is connected to the upper side of the motor 2, the fixing support 6 is arranged on the surface of the rotating shaft 5, the placing groove 7 is arranged on the surface of the fixing support 6, the clamping plate 8 is arranged inside the placing groove 7, the fixing spring 9 is connected to the tail end of the clamping plate 8, the fixing spring 9 is connected with the side wall of the placing groove 7, the lighting device 10 is arranged above the rotating shaft 5, the closed door 11 is arranged on the front side of the device shell 1, a sealing gasket 12 is arranged at the joint of the closing door 11 and the device shell 1, and an observation window 13 is arranged on the surface of the closing door 11.
The electric motor 2 is installed at the center of the ground inside the device shell 1, two ends of the electric motor 2 are respectively and fixedly connected with the device shell 1 and the rotating shaft 5, the hot air blower 3 is symmetrically arranged relative to the electric motor 2, and the hot air blower 3 is in contact connection with the electric motor 2, so that the hot air can be accelerated to rise.
The number of the fixing supports 6 is 8, the fixing supports 6 are uniformly distributed on the surface of the rotating shaft 5, the fixing supports 6 are staggered in a crossed mode and do not interfere with each other, the height of the rotating shaft 5 is smaller than the distance between the motor 2 and the lighting device 10, and therefore illumination required by the culture dish cannot be shielded.
2 standing groove 7 have been seted up to the upper surface of fixed bolster 6, and the clamp plate 8 passes through fixed spring 9 and moves the structure about constituting in standing groove 7, and the degree of depth that highly is greater than standing groove 7 of clamp plate 8 can be adjusted according to the size of culture dish.
The lighting device 10 comprises a device body 101, a sliding groove 102, a lighting lamp 103, a limiting block 104 and a handle 105, wherein the sliding groove 102 is arranged on the side wall inside the device body 101, the lighting lamp 103 is arranged inside the sliding groove 102, the limiting block 104 is arranged between the sliding grooves 102, and the handle 105 is connected to the tail ends of the lighting lamps 103 at the bottom and above the bottom of the device body 101.
The shape and size of the device main body 101 are the same as the shape and size of the inside of the device shell 1, the thickness of the illuminating lamp 103 and the distance between the sliding grooves 102 are equal, the width of the sliding grooves 102 is 2-5cm, the width of the limiting blocks 104 is equal to that of the sliding grooves 102, the handle 105 is fixedly connected with the illuminating lamp 103, and the strength of the illuminating lamp 103 can be adjusted according to the handle 105.
The working principle is as follows: when using this a cell culture device for bioreactor, at first, assemble the required spare part of device, clamp plate 8 on the fixed bolster 6 is opened, place the culture dish of adorning the nutrient solution in standing groove 7, clamp plate 8 is fixed the culture dish, when not needing high intensity illumination, lighting device 10 of device top only uses the light 103 of device main part 101 below to shine, starter motor 2, motor 2 slowly rotates, drive rotation axis 5 slowly rotates, make the inside nutrient solution of culture dish can not stand under the effect of centrifugal force, the effectual inside cell of having protected.
When high-intensity illumination is needed, the handle 105 is pulled, the illuminating lamp 103 at the lowest part of the device main body 101 and the middle position of the device main body 101 is pulled out from the sliding groove 102, so that the illuminating lamp 103 at the lower layer does not shield the illuminating lamp 103 at the upper layer, the illumination intensity is increased, when the illuminating lamp 103 needs to be moved back to the original position, the handle 105 is pushed to drive the illuminating lamp 103 to move inwards, the limiting blocks 104 are arranged at the lowest part of the device main body 101 and in front of the illuminating lamp 103 at the middle layer, when the illuminating lamp 103 touches the limiting blocks 104, which means that the illuminating lamp 103 returns to the original position, the hot air heater 3 at the lower part of the device shell 1 can heat the air in the device, the fan 4 is started, the air flow generated by the fan 4 drives the heat flow generated by the hot air heater 3 to accelerate to rise, which is the working process of the device, and the contents which are not described in detail in the specification are, such as the closed door 11, the closed door 103, the sliding groove 102, the sliding groove 103, the sliding groove 102, and the sliding groove 103 which are not described in detail in the description, The viewing windows 13, etc. are well known to those skilled in the art.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims (6)

1. A cell culture device for a bioreactor, comprising a device housing (1), a closure door (11), a sealing gasket (12) and an observation window (13), characterized in that: the device is characterized in that a motor (2) is arranged at the bottom end inside a shell (1), hot air blowers (3) are arranged at the two sides of the motor (2), a fan (4) is fixed above the hot air blowers (3), a rotating shaft (5) is connected to the upper side of the motor (2), a fixing support (6) is mounted on the surface of the rotating shaft (5), a placing groove (7) is formed in the surface of the fixing support (6), a clamping plate (8) is mounted inside the placing groove (7), a fixing spring (9) is connected to the tail end of the clamping plate (8), the fixing spring (9) is connected with the side wall of the placing groove (7), a lighting device (10) is arranged above the rotating shaft (5), a sealing door (11) is mounted on the front side of the shell (1), and a sealing gasket (12) is arranged at the connecting position of the sealing door (11) and the shell (1), an observation window (13) is arranged on the surface of the closed door (11).
2. A cell culture apparatus for a bioreactor according to claim 1, wherein: the air heater is characterized in that the motor (2) is installed at the center of the ground inside the device shell (1), two ends of the motor (2) are fixedly connected with the device shell (1) and the rotating shaft (5) respectively, the air heaters (3) are symmetrically arranged relative to the motor (2), and the air heaters (3) are in contact connection with the motor (2).
3. A cell culture apparatus for a bioreactor according to claim 1, wherein: the number of the fixing supports (6) is 8, the fixing supports (6) are uniformly distributed on the surface of the rotating shaft (5), the fixing supports (6) are staggered in a crossed mode and do not interfere with each other, and the height of the rotating shaft (5) is smaller than the distance between the motor (2) and the lighting device (10).
4. A cell culture apparatus for a bioreactor according to claim 1, wherein: 2 placing grooves (7) are formed in the upper surface of the fixing support (6), the clamping plate (8) forms a left-right moving structure in the placing grooves (7) through fixing springs (9), and the height of the clamping plate (8) is larger than the depth of the placing grooves (7).
5. A cell culture apparatus for a bioreactor according to claim 1, wherein: the lighting device (10) comprises a device body (101), a sliding groove (102), a lighting lamp (103), a limiting block (104) and a handle (105), the sliding groove (102) is installed on the side wall inside the device body (101), the lighting lamp (103) is installed inside the sliding groove (102), the limiting block (104) is arranged between the sliding grooves (102), and the tail ends of the lighting lamp (103) below and above the device body (101) are connected with the handle (105).
6. A cell culture apparatus for use in a bioreactor according to claim 5, wherein: the shape and size of the device main body (101) are the same as those of the inside of the device shell (1), the thickness of the illuminating lamp (103) is equal to the distance between the sliding grooves (102), the width of the sliding grooves (102) is 2-5cm, the width of the limiting blocks (104) is equal to that of the sliding grooves (102), and the handle (105) is fixedly connected with the illuminating lamp (103).
CN202022654855.XU 2020-11-17 2020-11-17 Cell culture device for bioreactor Active CN214458056U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022654855.XU CN214458056U (en) 2020-11-17 2020-11-17 Cell culture device for bioreactor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022654855.XU CN214458056U (en) 2020-11-17 2020-11-17 Cell culture device for bioreactor

Publications (1)

Publication Number Publication Date
CN214458056U true CN214458056U (en) 2021-10-22

Family

ID=78182738

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022654855.XU Active CN214458056U (en) 2020-11-17 2020-11-17 Cell culture device for bioreactor

Country Status (1)

Country Link
CN (1) CN214458056U (en)

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