CN114868746A - Bactericidal composition containing bronopol of alkaloid and alkaloid analogue - Google Patents
Bactericidal composition containing bronopol of alkaloid and alkaloid analogue Download PDFInfo
- Publication number
- CN114868746A CN114868746A CN202210365144.1A CN202210365144A CN114868746A CN 114868746 A CN114868746 A CN 114868746A CN 202210365144 A CN202210365144 A CN 202210365144A CN 114868746 A CN114868746 A CN 114868746A
- Authority
- CN
- China
- Prior art keywords
- bronopol
- alkaloid
- bactericidal composition
- test
- berberine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- LVDKZNITIUWNER-UHFFFAOYSA-N Bronopol Chemical compound OCC(Br)(CO)[N+]([O-])=O LVDKZNITIUWNER-UHFFFAOYSA-N 0.000 title claims abstract description 148
- 229960003168 bronopol Drugs 0.000 title claims abstract description 146
- 239000000203 mixture Substances 0.000 title claims abstract description 104
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 66
- 229930013930 alkaloid Natural products 0.000 title claims abstract description 48
- 150000003797 alkaloid derivatives Chemical class 0.000 title claims abstract description 48
- 229940093265 berberine Drugs 0.000 claims abstract description 50
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 claims abstract description 43
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000002360 preparation method Methods 0.000 claims abstract description 35
- 208000035143 Bacterial infection Diseases 0.000 claims abstract description 21
- ZHDBTKPXEJDTTQ-UHFFFAOYSA-N dipyrithione Chemical compound [O-][N+]1=CC=CC=C1SSC1=CC=CC=[N+]1[O-] ZHDBTKPXEJDTTQ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000284 extract Substances 0.000 claims abstract description 17
- 229960003811 pyrithione disulfide Drugs 0.000 claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 239000000575 pesticide Substances 0.000 claims abstract description 13
- 241000246044 Sophora flavescens Species 0.000 claims abstract description 10
- 239000004563 wettable powder Substances 0.000 claims description 40
- 239000000463 material Substances 0.000 claims description 29
- 241000196324 Embryophyta Species 0.000 claims description 28
- 239000004562 water dispersible granule Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 12
- 239000002270 dispersing agent Substances 0.000 claims description 10
- 239000000945 filler Substances 0.000 claims description 10
- 239000000080 wetting agent Substances 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 230000000855 fungicidal effect Effects 0.000 claims description 6
- 239000003094 microcapsule Substances 0.000 claims description 6
- 239000012752 auxiliary agent Substances 0.000 claims description 5
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 235000012055 fruits and vegetables Nutrition 0.000 claims description 3
- 239000002518 antifoaming agent Substances 0.000 claims description 2
- 238000010410 dusting Methods 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000004530 micro-emulsion Substances 0.000 claims description 2
- 231100001184 nonphytotoxic Toxicity 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 239000004548 suspo-emulsion Substances 0.000 claims description 2
- 239000002562 thickening agent Substances 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims 4
- 241000233866 Fungi Species 0.000 claims 1
- 239000004495 emulsifiable concentrate Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 claims 1
- 239000012053 oil suspension Substances 0.000 claims 1
- 230000003032 phytopathogenic effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 59
- 201000010099 disease Diseases 0.000 abstract description 32
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 32
- 230000002195 synergetic effect Effects 0.000 abstract description 18
- OVFHHJZHXHZIHT-UHFFFAOYSA-N 3-(2,4-dichlorophenyl)-2-(1,2,4-triazol-1-yl)quinazolin-4-one Chemical compound ClC1=CC(Cl)=CC=C1N1C(=O)C2=CC=CC=C2N=C1N1N=CN=C1 OVFHHJZHXHZIHT-UHFFFAOYSA-N 0.000 abstract description 12
- 230000009471 action Effects 0.000 abstract description 8
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000002688 persistence Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 99
- 241000207199 Citrus Species 0.000 description 90
- 235000020971 citrus fruits Nutrition 0.000 description 90
- 244000052616 bacterial pathogen Species 0.000 description 47
- 230000003385 bacteriostatic effect Effects 0.000 description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 31
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 description 27
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 description 27
- 229930014456 matrine Natural products 0.000 description 27
- 230000001580 bacterial effect Effects 0.000 description 25
- 241000894006 Bacteria Species 0.000 description 21
- 235000013399 edible fruits Nutrition 0.000 description 21
- 230000001018 virulence Effects 0.000 description 20
- 238000009472 formulation Methods 0.000 description 19
- RSNWORHVUOZYLT-UHFFFAOYSA-N 5-[[(2-sulfanylidene-3h-1,3,4-thiadiazol-5-yl)amino]methylamino]-3h-1,3,4-thiadiazole-2-thione Chemical compound S1C(=S)NN=C1NCNC1=NNC(=S)S1 RSNWORHVUOZYLT-UHFFFAOYSA-N 0.000 description 18
- 238000001514 detection method Methods 0.000 description 17
- 239000003899 bactericide agent Substances 0.000 description 16
- 230000009036 growth inhibition Effects 0.000 description 16
- 239000000546 pharmaceutical excipient Substances 0.000 description 16
- 239000004480 active ingredient Substances 0.000 description 15
- 238000007726 management method Methods 0.000 description 15
- 231100000419 toxicity Toxicity 0.000 description 15
- 230000001988 toxicity Effects 0.000 description 15
- 240000007594 Oryza sativa Species 0.000 description 14
- 235000007164 Oryza sativa Nutrition 0.000 description 14
- 238000013461 design Methods 0.000 description 14
- 238000011160 research Methods 0.000 description 14
- 235000009566 rice Nutrition 0.000 description 14
- 239000003814 drug Substances 0.000 description 13
- 150000001875 compounds Chemical class 0.000 description 12
- 238000002156 mixing Methods 0.000 description 12
- 231100000820 toxicity test Toxicity 0.000 description 12
- 238000013329 compounding Methods 0.000 description 11
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 10
- 229910052802 copper Inorganic materials 0.000 description 10
- 239000010949 copper Substances 0.000 description 10
- 238000011156 evaluation Methods 0.000 description 10
- 239000002420 orchard Substances 0.000 description 10
- 230000002265 prevention Effects 0.000 description 10
- 238000011835 investigation Methods 0.000 description 9
- 238000007405 data analysis Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 206010059866 Drug resistance Diseases 0.000 description 7
- 238000004364 calculation method Methods 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
- SRVJKTDHMYAMHA-WUXMJOGZSA-N thioacetazone Chemical compound CC(=O)NC1=CC=C(\C=N\NC(N)=S)C=C1 SRVJKTDHMYAMHA-WUXMJOGZSA-N 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 5
- 238000003912 environmental pollution Methods 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 5
- 238000003973 irrigation Methods 0.000 description 5
- 230000002262 irrigation Effects 0.000 description 5
- 230000007170 pathology Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000000375 suspending agent Substances 0.000 description 5
- 238000012795 verification Methods 0.000 description 5
- MFYSUUPKMDJYPF-UHFFFAOYSA-N 2-[(4-methyl-2-nitrophenyl)diazenyl]-3-oxo-n-phenylbutanamide Chemical compound C=1C=CC=CC=1NC(=O)C(C(=O)C)N=NC1=CC=C(C)C=C1[N+]([O-])=O MFYSUUPKMDJYPF-UHFFFAOYSA-N 0.000 description 4
- 235000007129 Cuminum cyminum Nutrition 0.000 description 4
- 244000304337 Cuminum cyminum Species 0.000 description 4
- 206010039509 Scab Diseases 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 241000219784 Sophora Species 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- XVPBINOPNYFXID-JARXUMMXSA-N 85u4c366qs Chemical compound C([C@@H]1CCC[N@+]2(CCC[C@H]3[C@@H]21)[O-])N1[C@@H]3CCCC1=O XVPBINOPNYFXID-JARXUMMXSA-N 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 3
- 239000012880 LB liquid culture medium Substances 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 241000589655 Xanthomonas citri Species 0.000 description 3
- 244000000005 bacterial plant pathogen Species 0.000 description 3
- 231100000026 common toxicity Toxicity 0.000 description 3
- 229930015582 oxymatrine Natural products 0.000 description 3
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 description 3
- 239000004308 thiabendazole Substances 0.000 description 3
- 229960004546 thiabendazole Drugs 0.000 description 3
- 235000010296 thiabendazole Nutrition 0.000 description 3
- QSOHVSNIQHGFJU-UHFFFAOYSA-L thiosultap disodium Chemical compound [Na+].[Na+].[O-]S(=O)(=O)SCC(N(C)C)CSS([O-])(=O)=O QSOHVSNIQHGFJU-UHFFFAOYSA-L 0.000 description 3
- FFISWZPYNKWIRR-UHFFFAOYSA-N 5-oxidophenazin-5-ium Chemical compound C1=CC=C2[N+]([O-])=C(C=CC=C3)C3=NC2=C1 FFISWZPYNKWIRR-UHFFFAOYSA-N 0.000 description 2
- 235000009436 Actinidia deliciosa Nutrition 0.000 description 2
- 244000298697 Actinidia deliciosa Species 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- 241000589565 Flavobacterium Species 0.000 description 2
- KYGZCKSPAKDVKC-UHFFFAOYSA-N Oxolinic acid Chemical compound C1=C2N(CC)C=C(C(O)=O)C(=O)C2=CC2=C1OCO2 KYGZCKSPAKDVKC-UHFFFAOYSA-N 0.000 description 2
- 244000131316 Panax pseudoginseng Species 0.000 description 2
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- FUHMZYWBSHTEDZ-UHFFFAOYSA-M bispyribac-sodium Chemical compound [Na+].COC1=CC(OC)=NC(OC=2C(=C(OC=3N=C(OC)C=C(OC)N=3)C=CC=2)C([O-])=O)=N1 FUHMZYWBSHTEDZ-UHFFFAOYSA-M 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000006184 cosolvent Substances 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000002050 diffraction method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000009916 joint effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 231100000397 ulcer Toxicity 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 239000005488 Bispyribac Substances 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 240000000560 Citrus x paradisi Species 0.000 description 1
- 235000005602 Coleus parviflorus Nutrition 0.000 description 1
- 240000004792 Corchorus capsularis Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000186427 Cutibacterium acnes Species 0.000 description 1
- 244000096857 Dioscorea batatas Species 0.000 description 1
- 235000002722 Dioscorea batatas Nutrition 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 235000007270 Gaultheria hispida Nutrition 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000220225 Malus Species 0.000 description 1
- 235000009134 Myrica cerifera Nutrition 0.000 description 1
- 244000269152 Myrica pensylvanica Species 0.000 description 1
- 235000012851 Myrica pensylvanica Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- RYVIXQCRCQLFCM-UHFFFAOYSA-N bispyribac Chemical compound COC1=CC(OC)=NC(OC=2C(=C(OC=3N=C(OC)C=C(OC)N=3)C=CC=2)C(O)=O)=N1 RYVIXQCRCQLFCM-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- FBOUIAKEJMZPQG-BLXFFLACSA-N diniconazole-M Chemical compound C1=NC=NN1/C([C@H](O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1Cl FBOUIAKEJMZPQG-BLXFFLACSA-N 0.000 description 1
- IZEKFCXSFNUWAM-UHFFFAOYSA-N dipyridamole Chemical compound C=12N=C(N(CCO)CCO)N=C(N3CCCCC3)C2=NC(N(CCO)CCO)=NC=1N1CCCCC1 IZEKFCXSFNUWAM-UHFFFAOYSA-N 0.000 description 1
- 229960002768 dipyridamole Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000010902 jet-milling Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 229960000321 oxolinic acid Drugs 0.000 description 1
- -1 pH regulators Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000003090 pesticide formulation Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactericidal composition of bronopol containing alkaloid and alkaloid analogue, a preparation method and an application scheme thereof. The invention provides a bactericidal composition of bronopol containing alkaloid and alkaloid analogue, which comprises alkaloid and alkaloid analogue component A and bronopol, wherein the alkaloid and alkaloid analogue component A is selected from one of sophora flavescens extracts, berberine, biscumin, quinconazole or dipyrithione. The weight part ratio of the alkaloid and alkaloid analogue component A to the bronopol is 119: 1-1: 39, and the weight percentage of the total weight of the alkaloid and alkaloid analogue component A and the bronopol in the bactericidal composition is 1% -90%. The composition has higher activity on a series of bacterial diseases of various crops, has obvious synergistic action, is superior to a single agent in quick action and persistence, reduces the using dosage and cost of pesticides, and is beneficial to environmental protection and comprehensive treatment of agricultural diseases.
Description
Technical Field
The invention relates to a pesticide sterilization composition and the application field thereof, in particular to a bronopol sterilization composition containing alkaloid and alkaloid analogue, a preparation method and an application scheme thereof, which are applied to preventing and treating crop bacterial diseases.
Background
Bronopol [ JEPTDQ Journal of Environmental Pathology and toxicology.1977,5(3)]CAS number: 52-51-7, English name: bronopol; chemical name: 2-bromo-2-nitro-1, 3-propanediol; the molecular formula is as follows: c 3 H 6 BrNO 4 (ii) a Relative molecular weight 199.9.
Bronopol is a high-efficiency broad-spectrum bactericide, is used for preventing and treating potato black shank, rice bakanae disease and kiwi fruit bacterial ulcer, and is especially widely applied to preventing and treating rice bakanae disease, researches on Scienguo et al [ pesticide 1989, (03) ] and Yuezongdai et al [ Jilin agricultural science 1989, (04) ] show that bronopol has obvious effect on preventing and treating rice bakanae disease, researches on Sun Ming et al [ Chinese potato 2020,34(04) ] show that bronopol has obvious effect on preventing and treating potato black shank, and researches on Wang Rui et al [ Jiangsu agricultural science 2019,47(11) ] show that bronopol has obvious effect on preventing and treating kiwi fruit bacterial ulcer.
Lightyellow sophora root extract (pharmacopoeia of the people's republic of China. Beijing: Chinese pharmaceutical science and technology Press, 2015), CAS number: 18837-52-8, is an alkaloid extracted from Sophora flavescens, wherein the active ingredients comprise: matrine (matrine), oxymatrine (oxymatrine), oxymatrine (sodium-anol), etc.
The kuh-seng extract is a broad-spectrum bactericide and has obvious inhibitory activity on staphylococcus aureus, staphylococcus epidermidis, propionibacterium acnes and other bacteria, research on guokavan and the like [ Guangdong chemical engineering, 2021,48(14) ] shows that the kuh-seng extract has obvious bacteriostatic effect on various bacteria, research on Zhangxuan (Tianjin medical university) shows that matrine has bacteriostatic effect on various bacteria, research on Duhuilan and the like [ Chinese herbal medicine, 2020,50(08) ] shows that the kuh-seng has bacteriostatic effect on various bacteria, and the inventor conjects that the kuh-seng extract or more bacteria exist for prevention and treatment. At present, no data report exists that the synergistic bacteriostatic action of the sophora flavescens extract and the bronopol on bacteria exists, the inventor obtains the synergistic bacteriostatic action of the sophora flavescens extract and the bronopol on the bacteriostatic activity of the bacteria through a toxicity test, and a field test proves that the feasibility of implementation of the combination exists.
Berberine (pharmacopoeia of the people's republic of China. Beijing: Chinese pharmaceutical science Press, 2015), CAS number: 2086-83-1, English name: bergine, molecular formula: c 20 H 18 NO 4 Relative molecular weight: 336.36.
berberine is a plant-extracted alkaloid which can be used for bacterial bacteriostasis and has a bacteriostasis effect on rice bacterial diseases and a bacteriostasis effect on staphylococcus aureus, researches on Yanping and the like [ the plant protection bulletin 2020,47(05) ] and Li Xiangjing (the university of Guangxi.2018) find that the berberine has a bacteriostasis effect on rice bacterial diseases, researches on Linjian and the like [ the Baotou medical college bulletin 2019,35(06) ] find that the berberine has a bacteriostasis effect on staphylococcus aureus, and the inventor conjects that the berberine or more bacteria prevention and treatment applications exist. At present, no data report that the berberine and the bronopol have the synergistic bacteriostatic action on bacteria, the inventor obtains the synergistic bacteriostatic action of the berberine and the bronopol on the bacteriostatic activity of the bacteria through toxicity tests, and the field tests prove that the combination has the implementation feasibility.
Leaf blight [ Journal of Chemical crystallography.2013,43(10)]The CAS number: 304-81-4, English name: phenazine oxide, chemical name: 5-oxyphenazine, formula: c 12 H 8 N 2 O, relative molecular weight: 196.2.
the leaf blight is a bactericide which has a special effect on rice bacterial blight, the research on elements of southern opening university and the like [ pesticide industry, 1974, (04) ] finds that the leaf blight has a special effect on the rice bacterial blight, the research on Xie Wen's writing [ pesticide industry, 1974(Z1) ] finds that the leaf blight has a remarkable effect on the rice bacterial blight, and the inventor conjectures that the leaf blight or more bacteria exist for prevention and treatment. Until now, no data report that the synergistic bacteriostatic action of the bismerthiazol and the bronopol on bacteria exists, the inventor obtains the synergistic bacteriostatic action of the bismerzin and the bronopol on the bacteriostatic activity of the bacteria through a toxicity test and proves the feasibility of the implementation of the combination through a field test.
Quinazone [ EUROPEAN pharopoeia 6.0.2008,01], CAS number: 14698-29-4, english name: oxolinic acid.
The quinazone is a bactericide and has a control effect on rice bacterial brown grains, fire blight and potato bacterial diseases, the quinazone is found to have a control effect on the rice bacterial brown grains by research of Quchenmin and the like [ modern agriculture.2014, (06) ], the quinazone is found to have a control effect on the rice bacterial brown grains by research of D Shitenberg and the like [ phytoparasitica.2001], the quinazone is found to have a control effect on the fire blight by research of S Hamed and the like [ J.plant prot.and Path, Mansoura Univ.2018,9(8) ], and the inventor conjects that the leaf blight or more bacteria exist for control and application. Until now, no data report that the synergistic bacteriostatic action of the quinconazole and the bronopol on bacteria exists, the inventor obtains the synergistic bacteriostatic action of the quinconazole and the bronopol on the bacteriostatic activity of the bacteria through a toxicity test and proves the feasibility of the implementation of the combination through a field test.
Piripercus bispirostris (The Journal of Organic chemistry.1980), CAS number: 3696-28-4, English name: 2,2' -Dithiobis (pyridine-N-oxide); the molecular formula is as follows: c 10 H 8 N 2 O 2 S 2 (ii) a Relative molecular weight: 252.31. at present, no data report shows that the dipyrithione has bacteriostatic activity on plant pathogenic bacteria, the inventor obtains the bacteriostatic data of the dipyrithione on the plant pathogenic bacteria through a toxicity test, knows that the dipyrithione and the bronopol have synergistic effect on the bacteriostatic activity of the bacteria through the toxicity test, and proves that the composition has feasibility of implementation through a field efficacy test.
Agricultural bacterial diseases refer to plant diseases caused by bacterial pathogen infection, such as soft rot, canker, bacterial wilt and the like. Bacterial diseases are important factors influencing the agricultural production value, and the application advantages of the composition in citrus bacterial diseases are shown by taking citrus bacterial diseases as an example.
Citrus canker (citrus canker) is a major epidemic disease affecting the production of citrus in the world, the causative bacterium being Xanthomonas carpi citrus var (xanthmonas Campestris pv. citri) [ topical Plant Pathology.2012,37(1) ]. Citrus canker is currently distributed mainly in more than 30 countries and regions in asia, africa and america [ European Journal of Plant Pathology.2012,133(3) & Plant Pathology,2012,61(4) ]. China, Japan, the United states and other major countries occur, which account for one third of the countries and regions where citrus is produced worldwide, and Asian countries are more common [ Plant Pathology journal.2012,28(2) & PLoS ONE.2012,7(7) & Acta Crystagonorrhaphy Section D-Biological crystallography.2012,68 ]. Among them, in 1995 to 2000, citrus canker lesions in florida, usa, resulted in annual delivery reduction of $ 1.8 million $ [ African Journal of biotechnology.2011,10(19) & Indian Journal of microbiology.2011,51(2) ]. It is apparent that citrus canker directly affects citrus yield. Therefore, the inventor carries out experimental analysis on pathogenic bacteria of citrus canker based on NY/T1156.16-2008 & turbidity method for bactericide test for inhibiting bacterial growth & GB/T17980.103-2004 & guidelines for pesticide field efficacy test (II) part 103: the experiment analysis of the bactericide for preventing and treating citrus canker in the field is carried out on the field for preventing and treating the canker of citrus trees. Wherein, the agricultural industry standard NY/T1156.16-2008 < bacteriocide inhibition bacteria growth quantity test turbidity method > of the people's republic of China provides an operation basis and a methodology for the toxicity determination embodiment of the invention; the national standard GB/T17980.103-2004 'pesticide field efficacy test criterion (II) part 103 of the people's republic of China: the bactericide for preventing and treating citrus canker provides an operation basis and a methodology for the application embodiment of the pesticide effect in the invention field.
The compounding of bactericides of different types, particularly different action mechanisms is a common method for preventing and controlling agricultural resistant diseases. On the basis of a large amount of indoor toxicity, preparation formulations and field efficacy tests, the compound of the sophora flavescens extract, berberine, bismerthiazol, quinconazole, bispyrthion and bronitol is deeply researched, and the discovery that the compound of one of the sophora flavescens extract, the berberine, the bismerthiazol, the quinconazole or the bispyrthion and the bronitol has obvious synergistic interaction in a certain proportion range and has good control effect on crop bacterial diseases, and the invention is completed through further research.
Disclosure of Invention
The invention aims to provide a bactericidal composition with obvious synergistic control effect on crop diseases.
The invention aims to apply the bactericidal composition to control crop bacterial diseases.
The object of the present invention is to provide a more efficient bacterial disease control solution.
The above object of the present invention is achieved by the following technical solutions:
the invention provides a bactericidal composition of bronopol containing alkaloid and alkaloid analogue, which comprises alkaloid and alkaloid analogue component A and bronopol, wherein the alkaloid and alkaloid analogue component A is one selected from sophora flavescens extracts, berberine, cumin, quinone or dipyrithione, the weight part ratio of the alkaloid and alkaloid analogue component A to the bronopol is 119: 1-1: 39, and the total weight of the alkaloid and alkaloid analogue component A and the bronopol accounts for 1-90% of the total weight of the bactericidal composition.
In a further preferable scheme, the bactericidal composition comprises an alkaloid and alkaloid analogue component A and bronopol, wherein the alkaloid and alkaloid analogue component A is one selected from sophora flavescens extracts, berberine, cumicin, quinconazole or dipyrithione, the weight part ratio of the alkaloid and alkaloid analogue component A to the bronopol is 99: 1-1: 19, and the total weight percentage of the alkaloid and alkaloid analogue component A and the bronopol in the bactericidal composition is 1-80%.
The bactericidal composition can be prepared into wettable powder, soluble powder, water dispersible granules, soluble granules, aqueous emulsion, microemulsion, missible oil, suspending agent, dispersible oil suspending agent, microcapsule suspending agent, soluble solution, suspoemulsion, suspending agent-microcapsule suspending agent and aqueous emulsion-microcapsule suspending agent.
The bactericidal composition further comprises common auxiliary agents required for preparing pesticide preparations, wherein the common auxiliary agents are one or a mixture of several of solvents, emulsifiers, wetting agents, stabilizers, dispersants, thickeners, pH regulators, antifoaming agents, antifreezing agents, filling agents and the like, are known substances, are various auxiliary agents commonly used in pesticide preparations (Liu Guang, and the like, modern pesticide formulation processing technology book [ D ] chemical industry Press, Beijing.), can be changed according to different conditions, and are not particularly limited.
The bactericidal composition provided by the invention can be used for preventing and treating bacterial diseases of crops, wherein the crops are selected from grain crops, economic crops, fruits and vegetables and lawn garden crops, the grain crops comprise corn, rice, wheat and the like, the economic crops comprise peanut, cotton, rape, pseudo-ginseng, ginseng and the like, the fruits and vegetables comprise orange, apple, grapefruit, pear, waxberry, tomato, eggplant, hot pepper, cabbage, ginger and the like, and the lawn garden crops comprise flowers, plants and trees on lawns.
The bactericidal composition provided by the invention can be used for preventing and treating crop bacterial diseases, wherein the bacterial diseases comprise rice bacterial brown spot, corn bacterial streak, cucumber bacterial angular leaf spot, Chinese cabbage bacterial angular leaf spot and the like, bacterial leaf blight of rice, cucumber bacterial leaf blight, melon bacterial leaf blight, tomato bacterial wilt, potato bacterial wilt, strawberry bacterial wilt and the like, citrus canker, soybean bacterial leaf spot, tomato bacterial leaf spot, Chinese cabbage bacterial soft rot, chrysanthemum root cancer and the like.
The invention provides a bactericidal composition containing bronopol, which is prepared by applying active ingredient A and bronopol to plant pathogenic bacteria and/or environment thereof, or plant, plant part, plant propagation material and plant organ, soil or cultivation medium, material or space grown later, wherein the active ingredient A is selected from one of sophora flavescens extract, berberine, cumin, quinconazole or bispyrifos.
The present invention provides a fungicidal composition comprising bronopol, typically applied by spraying, and optionally using other techniques of application in agriculture, typically applied to plants, plant propagation material and plant organs, cultivation media, materials or spaces that grow subsequently, in an agronomically effective and substantially non-phytotoxic application rate, by seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, scattering or fuming.
Compared with the prior art, the invention has the advantages that:
1. one of the lightyellow sophora root extract, the berberine, the bismerthiazol, the quinconazole or the bispyribac-sodium is compounded with the bronopol, so that the synergistic effect is remarkable, and the prevention and treatment effect on germs is improved compared with the single agent used alone.
2. One of the lightyellow sophora root extract, the berberine, the bismerthiazol, the quinconazole or the bispyribac-sodium and the bronopol have different action mechanisms, and the combination can delay the generation of drug resistance of germs.
3. One of the lightyellow sophora root extract, the berberine, the bismerthiazol, the quinconazole or the dipyridamole is compounded with two effective components of the bronopol, so that the use amount of the pesticide is reduced, the use cost is reduced, the pollution to the environment is lightened, and the direction of reducing and increasing the quantity of the pesticide is met.
Detailed Description
In order to better understand the essence of the present invention, the following examples are further described, but should not be construed as limiting the present invention, the contents mentioned in the examples are not limiting the present invention, and the material formulation can be selected according to local conditions without substantial influence on the result. In these examples, all percentages are by weight unless otherwise indicated.
The indoor activity determination method comprises the following steps:
the inventors have conducted extensive target tests, exemplified by a Flavobacterium carproplasma citrus virulence test (Xanthomonas Campestris pv. citri) to demonstrate indoor activity assays.
The test is carried out by referring to agricultural industry standard NY/T1156.16-2008 ' Bactericide growth inhibition amount test turbidity method ' of the people's republic of China:
1. the purpose of the test is as follows: screening the indoor toxicity of the pathogenic bacteria of the bacterial diseases by using different compound combinations;
2. conditions of the experiment
2.1, test target: flavobacterium carpeting (Xanthomonas Campestris pv. citri)
2.2, culture conditions: LB liquid culture medium, temperature 28.0-30.0 deg.C;
2.3, experimental apparatus: an electronic balance, a shaking incubator, a culture dish, a conical flask, a liquid-transfering gun, a coater and a constant-temperature incubator;
3. test method
3.1, preparing mother liquor, and preparing treatment concentration according to each treatment for later use;
3.2, sequentially sucking 3.00mL of liquid medicine from low concentration to high concentration according to the experimental design, adding the liquid medicine into 27.0mL of LB liquid culture medium, and repeating the treatment for 3 times; and the final concentration of each treatment is respectively required concentration;
3.3, taking 100.0 mu L of bacteria liquid of the activated fresh bacteria liquid into an LB liquid culture medium;
3.4 before starting the culture, 3.00mL of each of the samples was collected and measured for OD 600 Values as drug controls for each treatment;
3.5, placing the mixture into a constant temperature shaking (180rpm) of 28.0 ℃ for culturing for 24 hours.
4. The test investigation and calculation method comprises the following steps:
the turbidity of each treatment was measured before the start of the incubation and recorded when the control treatment reached the logarithmic phase.
Theoretical virulence index of mixture
The virulence index of the A medicament is multiplied by the percentage of the A medicament in the mixed preparation and the virulence index of the B medicament is multiplied by the percentage of the B medicament in the mixed preparation
The theoretical virulence index of the mixed preparation is equal to the sum of the virulence indexes of the single preparations and the products of the virulence indexes and the contents of the single preparations in the mixed preparation, the cotoxicity coefficient is greater than 120 and is synergistic, the cotoxicity coefficient is greater than or equal to 80 and is less than or equal to 120 and is additive, and the cotoxicity coefficient is less than 80 and is antagonistic.
Indoor activity assay example 1: bronopol and radix Sophorae Flavescentis extract
According to the verification of a large amount of mixing tests of the inventor, 5 ratios of 99:1, 49:1, 9:1, 1:1 and 1:9 are taken for carrying out virulence test and displaying, and the experimental dose after pre-test is designed as shown in table 1:
TABLE 1 test dosage design of bronopol and matrine
Toxicity test turbidity tests were performed at the doses shown in Table 1 to calculate the OD in each cell 600 Obtaining the growth inhibition rate of each cell by data analysis, analyzing the growth inhibition rate of the single dose of the bronopol and the matrine to speculate the toxicity baseline and EC of the bronopol and the matrine 50 The values are shown in table 2:
TABLE 2 indoor toxicity determination of nitrobromohydrin and matrine to pathogenic bacteria of citrus canker
As can be seen from Table 2, bronopol and matrine have bacteriostatic activity against citrus canker pathogenic bacteria, wherein bronopol has bacteriostatic activity against citrus canker pathogenic bacteria EC 50 38.23mg/L, antibacterial activity EC of matrine to pathogenic bacteria of citrus canker disease 50 Was 112..35 mg/L.
Performing turbidity test on the medicament doses shown in Table 1 to obtain the growth inhibition rates of 5 doses of 5 ratios of the bronopol and the matrine on the pathogenic bacteria of the citrus canker disease, and analyzing to obtain the virulence baseline and EC of each ratio 50 The value results are shown in table 3:
virulence determination of citrus canker by using 35 compounding ratios in table
As shown in Table 3, the combination of bronopol and matrine has bacteriostatic activity against pathogenic bacteria of citrus canker. EC of each proportion 50 Is 24.61-98.82mg/L, wherein the ratio of 99:1 has the highest activity, EC 50 It was 24.61 mg/L.
The preparation EC is compounded by each group in the table 3 50 The data analysis is carried out to obtain the evaluation of the combined action of 5 compounding ratios of the bronopol and the matrine on pathogenic bacteria of citrus canker disease, which is shown in table 4:
TABLE 4 evaluation of the combination of 5 compounding ratios of bronopol and matrine on pathogenic bacteria of citrus canker
As can be seen from Table 4, the cotoxicity coefficient of 5 proportions of bronopol and matrine is between 86.73 and 156.36, and the joint action modes of different proportions on pathogenic bacteria of citrus canker are different, wherein the ratio of bronopol: the matrine ratio is 99:1, and the high co-toxicity index value is 156.36
Tests after the matrine and the bronopol are mixed show that the matrine and the bronopol have synergy, and the combined use can delay the drug resistance of the citrus canker, improve the control effect, reduce the environmental pollution, save the resources and reduce the agricultural cost of the product.
Indoor activity assay example 2: bronopol and berberine
According to the verification of a large amount of mixing tests of the inventor, 5 ratios of 15:1, 10:1, 5:1, 1:1 and 1:5 are taken for carrying out virulence test and displaying, and the experimental dose after pre-test is designed as shown in table 5:
TABLE 5 Experimental dose design of bronopol and berberine
Toxicity test experiments turbidity tests were performed at the doses shown in Table 5 to calculate the OD in each cell 600 Obtaining the growth inhibition rate of each cell through data analysis, analyzing the growth inhibition rates of the bronopol and the berberine to speculate the toxicity baseline and the EC of the bronopol and the berberine 50 The values are shown in Table 6:
TABLE 6 indoor toxicity determination of nitrobromohydrin and berberine on pathogenic bacteria of citrus canker
As can be seen from Table 6, bronopol and berberine have bacteriostatic activity against pathogenic bacteria of citrus canker disease, wherein bronopol has bacteriostatic activity against pathogenic bacteria of citrus canker disease EC 50 39.01mg/L, berberine has bacteriostatic activity on pathogenic bacteria of citrus canker disease EC 50 It was 62.08 mg/L.
Turbidity tests are carried out on the medicament doses shown in the table 5 to obtain the growth inhibition rate of 5 doses of 5 ratios of the bronopol and the berberine on pathogenic bacteria of citrus canker, and the toxicity baseline and the EC of each ratio are analyzed and estimated 50 The value results are shown in table 7:
virulence determination of citrus canker by using 75 compounding ratios in table
As can be seen from Table 7, the combination of bronopol and berberine has bacteriostatic activity against pathogenic bacteria of citrus canker. EC of each proportion 50 28.99-63.67mg/L, wherein the ratio of 5:1 has the highest activity, EC 50 It was 28.99 mg/L.
The preparation EC is compounded from the groups of table 7 50 The evaluation of the combined action of 5 compounding ratios of bronopol and berberine on citrus canker pathogenic bacteria obtained by analyzing the value data is shown in table 8:
TABLE 8 evaluation of the combination of 5 combinations of bronopol and berberine for pathogenic bacteria of citrus canker
As can be seen from Table 8, the cotoxicity coefficient of 5 proportions of bronopol and berberine is between 88.75 and 143.44, and the joint action modes of different proportions of bronopol and berberine on pathogenic bacteria of citrus canker are different, wherein, the ratio of bronopol: the berberine has the highest synergistic effect at the ratio of 5:1, and the co-toxicity index value is 143.44.
Tests after the berberine and the bronopol are mixed show that the berberine and the bronopol have synergism, and the combined use can delay the drug resistance of the citrus canker, improve the control effect, reduce the environmental pollution, save the resources and reduce the agricultural cost of the product.
Indoor activity assay example 3: bronopol and bismerthiazol
According to the verification of a large amount of mixing tests of the inventor, 5 ratios of 15:1, 10:1, 5:1, 1:1 and 1:5 are taken for carrying out virulence test and displaying, and the experimental dose after pre-test is designed as shown in a table 9:
TABLE 9 bronopol and bismerthiazol test dosage design
Toxicity test turbidity tests were performed at the doses shown in Table 9 to calculate the OD of each cell 600 Obtaining the growth inhibition rate of each cell through data analysis, analyzing the growth inhibition rate of the single agent of the bronopol and the bisultyn to conjecture the virulence baseline and the EC of the bronopol and the bisultyn 50 The values are shown in Table 10:
TABLE 10 indoor toxicity determination of nitrobromohydrin and bismerthiazol on pathogenic bacteria of citrus canker
As can be seen from Table 10, bronopol and bismeryn have bacteriostatic activity against pathogenic bacteria of citrus canker, in which bronopol has bacteriostatic activity against pathogenic bacteria of citrus canker EC 50 39.91mg/L, and the bacteriostatic activity of the leaf blight to pathogenic bacteria of citrus canker disease EC 50 It was 125.69 mg/L.
Turbidity tests are carried out on the medicament doses shown in the table 9 to obtain the growth inhibition rates of 5 doses of 5 ratios of the bronopol and the bisultap on pathogenic bacteria of citrus canker disease, and the toxicity baseline and the EC of each ratio are analyzed and estimated 50 The value results are shown in table 11:
virulence determination of citrus canker in table 115 compounding ratios
As can be seen from Table 11, the combination of bronopol and bismerthiazol has bacteriostatic activity against pathogenic bacteria of citrus canker. EC of each proportion 50 29.05-103.21 mg/L, wherein the ratio is 5:1 activity is optimal, EC 50 It was 29.05 mg/L.
The preparation EC is compounded from the groups of table 11 50 The evaluation of the combined action of 5 compounding ratios of bronopol and bismerthiazol on pathogenic bacteria of citrus canker disease obtained by analyzing the value data is shown in table 12:
TABLE 12 evaluation of the combination of 5 compounding ratios of bronopol and bisultap on pathogenic bacteria of citrus canker
As can be seen from Table 12, the cotoxicity coefficient of 5 mixtures of bronopol and bismerthiazol is between 86.86 and 155.02, wherein the ratio of bronopol: the ratio of the bismerthiazol to the bismerthiazol is 5:1, the synergistic effect is the highest, and the common toxicity index value is 155.02.
The tests of the mixture of the bismerthiazol and the bronopol show that the two have synergy, and the combined use can delay the drug resistance of the citrus canker, improve the control effect, reduce the environmental pollution, save the resources and reduce the agricultural cost of the product.
Indoor activity assay example 4: bronopol and quinconazole
According to the verification of a large amount of mixing tests of the inventor, 5 ratios of 15:1, 10:1, 5:1, 1:1 and 1:5 are taken for carrying out virulence test and displaying, and the experimental dose after pre-test is designed as shown in a table 13:
TABLE 13 Brornitrol and Quillatone test dose design
Toxicity test turbidity tests were performed at the doses shown in Table 13 to calculate the OD of each cell 600 Obtaining the growth inhibition rate of each cell through data analysis, analyzing the growth inhibition rate of the single dose of the bronopol and the copper thiediazole to conjecture the virulence baseline and EC of the bronopol and the copper thiediazole 50 The values are shown in Table 14:
TABLE 14 indoor toxicity determination of bronopol and quinidone to pathogenic bacteria of citrus canker
As can be seen from Table 14, bronopol and thiediazole copper have bacteriostatic activity against pathogenic bacteria of citrus canker, wherein bronopol has bacteriostatic activity against pathogenic bacteria of citrus canker EC 50 40.88mg/L, the bacteriostatic activity EC of the thiediazole copper on pathogenic bacteria of citrus canker disease 50 It was 48.57 mg/L.
Turbidity tests are carried out on the medicament doses shown in the table 13 to obtain the growth inhibition rates of 5 doses of 5 ratios of the bronopol and the thiabendazole copper on the pathogenic bacteria of the citrus canker, and the toxicity baseline and the EC of each ratio are analyzed and estimated 50 The value results are shown in table 15:
virulence determination of citrus canker by using 155 compound ratios in table
As can be seen from Table 15, the combination of bronopol and thiediazole copper has bacteriostatic activity against pathogenic bacteria of citrus canker. EC of each proportion 50 The content of the active carbon is 34.06-49.13 mg/L, wherein the ratio is 5:1 activity is optimal, EC 50 It was 34.06 mg/L.
The compound preparation EC of each group in the table 15 50 The data analysis is carried out to obtain the evaluation of the combined action of 5 compound ratios of the bronopol and the thiediazole copper on the pathogenic bacteria of the citrus canker disease, which is shown in the table 16:
TABLE 16 evaluation of the combination of 5 compounding ratios of bronopol and quinconazole on pathogenic bacteria of citrus canker
As can be seen from Table 16, the cotoxicity coefficient of 5 mixtures of bronopol and thiabendazole is between 97.70 and 138.25, wherein the molar ratio of bronopol: the ratio of the thiediazole copper is 5:1, the synergistic effect is the highest, and the common toxicity index value is 138.25.
The tests after the blending of the thiabendazole copper and the bronopol show that the two have synergism, and the combined use can delay the drug resistance of the citrus canker, improve the control effect, reduce the environmental pollution, save the resources and reduce the agricultural cost of the product.
Indoor activity assay example 5: bronopol and dipyrithione
According to the verification of a large amount of mixing tests of the inventor, 5 ratios of 15:1, 10:1, 5:1, 1:1 and 1:5 are taken for carrying out virulence test and displaying, and the experimental dose after pre-test is designed as shown in a table 17:
TABLE 17 design of the test dosage of bronopol and bispyrithione
Toxicity test turbidity tests were performed at the doses shown in Table 17 to calculate the OD of each cell 600 Obtaining the growth inhibition rate of each cell through data analysis, analyzing the growth inhibition rates of the bronopol and the bispyrisulum single agent to conjecture the virulence baseline and EC of the bronopol and the bispyrisulum 50 The values are shown in Table 18:
TABLE 18 determination of indoor toxicity of nitrobromohydrin and dipyrithione on pathogenic bacteria of citrus canker
As can be seen from Table 18, bronopol and dipyrithione have bacteriostatic activity against pathogenic bacteria of citrus canker, wherein bronopol has bacteriostatic activity against pathogenic bacteria of citrus canker EC 50 40.88mg/L, the bacteriostatic activity EC of the thiediazole copper on pathogenic bacteria of citrus canker disease 50 It was 76.96 mg/L.
Turbidity tests are carried out on the medicament doses shown in the table 17 to obtain the growth inhibition rate of 5 doses of 5 ratios of the bronopol and the bispyrithion on pathogenic bacteria of citrus canker, and the toxicity baseline and the EC of each ratio are analyzed and estimated 50 The value results are shown in table 19:
toxicity determination of table 195 compound ratios on citrus canker
As can be seen from Table 19, after combining bronopol and dipyrithione, each compound had bacteriostatic activity against the pathogenic bacteria of citrus canker. EC of each proportion 50 43.00-73.56 mg/L, wherein the ratio is 5:1 activity is optimal, EC 50 The concentration was 43.00 mg/L.
The preparation EC is compounded from the components of table 19 50 The data analysis is carried out to obtain the evaluation of the combined action of 5 compound ratios of the bronopol and the bispyrithion on pathogenic bacteria of citrus canker disease, which is shown in table 20:
TABLE 20 evaluation of the combination of 5 compounding ratios of bronopol and bispyrithion for pathogenic bacteria of citrus canker
As can be seen from Table 20, the cotoxicity coefficient of 5 mixtures of bronopol and bispyrithiuron is 86.56-156.02, wherein the ratio of bronopol: the ratio of the bispyrithiuron to the total amount is 5:1, the synergistic effect is the highest, and the common toxicity index value is 156.02.
The tests of the mixed dipyrithione and bronopol show that the dipyrithione and bronopol have synergy, and the combined use of the dipyrithione and the bronopol can delay the drug resistance of citrus canker, improve the control effect, reduce the environmental pollution, save the resources and reduce the agricultural cost of the product.
According to the toxicity test result, the inventor prepares the compound preparation, and selects a part of the compound preparation for result display.
Formulation excipient example 1: 20% bronopol-matrine soluble agent (19.8:0.2)
The composition of 20% bronopol matrine soluble solution is shown in table 21:
table 2120% bronopol matrine soluble component
According to the specification of each component in table 21, the effective component 2, the cosolvent and the emulsifier are sequentially put into a stirring kettle and mixed for 10min, after the materials are uniformly clarified, the effective component 1, the solvent and the defoamer are added and continuously stirred for 10min until the materials are completely uniformly clarified, so that the 20% bronopol-matrine soluble solution of the bactericidal composition can be prepared, and the standard detection result of the 20% bronopol-matrine soluble solution of the bactericidal composition is shown in table 22:
detection details of indexes of table 2220% bronopol-matrine soluble solution
As shown in Table 22, the bactericidal composition of the present invention, 20% bronopol matrine soluble agent, satisfies various standards of the product, and is a qualified product.
Formulation excipient example 2: 25% bronopol-matrine wettable powder (24.5:0.5)
The composition of 25% bronopol matrine wettable powder is shown in table 23:
TABLE 2325% wettable powder of bronopol and matrine
According to the specification of each component in table 23, a carrier and an active ingredient 2 are mixed for 10min by a gravity-free mixer, the active ingredient 1, a wetting agent, a dispersing agent and a filler are sequentially added, after the mixing for 10min, the mixed material is subjected to airflow crushing, and the D98 is not more than 18 μm, so that the 25% bronopol-matrine wettable powder of the bactericidal composition can be prepared, and the product standard detection result of the 25% bronopol-matrine wettable powder of the bactericidal composition is shown in table 24:
TABLE 2425% Denitritol matrine wettable powder for various indexes
As can be seen from Table 24, the 25% bronopol matrine wettable powder of the bactericidal composition meets various standards of products, and is a qualified product.
Product formulation excipient example 3: 60% bronopol berberine water dispersible granule (50:10)
The composition of the 60% bronopol berberine water dispersible granule is shown in table 25:
table 2560% bronopol berberine water dispersible granule component
According to the specification of each component in table 25, the effective component 1, the effective component 2, the dispersing agent, the wetting agent and the filler are put into a screw mixer to be mixed for 10min, the materials are subjected to airflow crushing until the D98 is less than or equal to 18 mu m, the crushed materials are mixed with water (60-65 kg of deionized water is used for each ton of the materials) by the mixer to enable the materials to have plasticity, the uniformly mixed materials are extruded into columnar particles with the diameter of 0.8mm, and the columnar particles are dried by hot air at 90 ℃ until the moisture content is less than 2%, so that the 60% bronopol-berberine water dispersible granule of the sterilization composition can be prepared. The product standard detection result of the 60% bronopol berberine water dispersible granule of the bactericidal composition of the invention is shown in table 26:
table 2660% bronopol-berberine water dispersible granule index detection details
As shown in Table 26, the 60% bronopol berberine water dispersible granule of the bactericidal composition meets all standards of products, and is a qualified product.
Product formulation excipient example 4: 22% bronopol-berberine soluble solution (20:2)
The 22% bronopol berberine soluble solution composition is shown in table 27:
2722% of bronopol-berberine soluble solution component detail
According to the specification of each component in table 27, the effective component 2, the cosolvent and the emulsifier are sequentially put into a stirring kettle and mixed for 10min, after the materials are uniformly clarified, the effective component 1, the solvent and the defoamer are added and continuously stirred for 10min until the materials are completely uniformly clarified, so that the bactericidal composition 22% bronopol-berberine soluble solution can be prepared, and the product standard detection result of the bactericidal composition 22% bronopol-berberine soluble solution is shown in table 28:
2822% of bronopol-berberine soluble solution and its index detection details
According to the table 28, the bactericidal composition 22% of the bronopol-berberine soluble solution meets all the standards of the product, and is a qualified product.
Product formulation excipient example 5: 66% bronopol-bismerthiazol water dispersible granule (55:11)
The composition of 66% bronopol leaf blight cleaning water dispersible granule is shown in table 29:
TABLE 2966% bronopol-bismerthiazol water dispersible granule
According to the specification of each component in table 29, the effective component 1, the effective component 2, the dispersing agent, the wetting agent and the filler are put into a screw mixer to be mixed for 10min, the materials are subjected to jet milling until the D98 is less than or equal to 18 mu m, the crushed materials are mixed with water (55-58 kg of deionized water is used for each ton of the materials) by the mixer to enable the materials to have plasticity, the uniformly mixed materials are extruded into columnar particles with the diameter of 0.8mm, and the columnar particles are dried by hot air at 90 ℃ until the moisture content is less than 2%, so that the 66% bronopol-leaf withered water dispersible granule of the sterilization composition can be prepared. The product standard detection result of 66% bronopol leaf blight cleaning water dispersible granules of the bactericidal composition is shown in table 30:
TABLE 3066% bronopol-bismerthiazol water dispersible granule
As can be seen from Table 30, 66% of the bronopol-bismerthiazol water dispersible granule of the bactericidal composition meets all standards of products, and is a qualified product.
Product formulation excipient example 6: 66% bronopol, bisultap wettable powder (60:6)
The composition of 66% bronopol bismerthiazol wettable powder is shown in table 31:
table 3166% bronopol-bismerthiazol wettable powder with fine composition
According to the specification of each component in table 31, a carrier and an active ingredient 2 are mixed for 10min by a gravity-free mixer, the active ingredient 1, a wetting agent, a dispersing agent and a filler are sequentially added, after the mixing for 10min, the mixed material is subjected to airflow crushing, and the D98 is not more than 18 μm, so that 66% of bronopol and leaf cumin wettable powder of the bactericidal composition can be prepared, and the product standard detection result of the 66% of bronopol and leaf cumin wettable powder of the bactericidal composition is shown in table 32:
table 3266% bronopol, bismerthiazol wettable powder various index detection details
As can be seen from Table 32, the 66% bronopol leaf cumic wettable powder of the bactericidal composition meets various standards of products and is a qualified product.
Product formulation excipient example 7: 72% bronopol-thiacetone wettable powder (60:12)
The composition of 72% bronopol thiacetone wettable powder is shown in Table 33:
TABLE 3372% wettable powder of bronopol-thiacetone
According to the specification of each component in table 33, a carrier and an active ingredient 2 are mixed for 10min by a gravity-free mixer, the active ingredient 1, a wetting agent, a dispersing agent and a filler are sequentially added, after the mixing for 10min, the mixed material is subjected to airflow crushing, and the D98 is not more than 18 μm, so that 72% bronopol-thiacetone wettable powder of the bactericidal composition can be prepared, and the product standard detection result of the 72% bronopol-thiacetone wettable powder of the bactericidal composition is shown in table 34:
table 3472% bronopol thiacetone wettable powder various indexes detection details
As can be seen from Table 34, the 72% bronopol thiacetone wettable powder of the bactericidal composition meets various standards of products and is a qualified product.
Product formulation excipient example 8: 72% bronopol-thiacetone wettable powder (36:36)
The composition of 72% bronopol thiacetone wettable powder is shown in table 35:
table 3572% bronopol-thiacetone wettable powder component
According to the specification of each component in table 35, a carrier and an active ingredient 2 are mixed for 10min by a gravity-free mixer, the active ingredient 1, a wetting agent, a dispersing agent and a filler are sequentially added, after the mixing for 10min, the mixed material is subjected to airflow crushing, and the D98 is not more than 18 μm, so that 72% bronopol-thiacetone wettable powder of the bactericidal composition can be prepared, and the product standard detection result of the 72% bronopol-thiacetone wettable powder of the bactericidal composition is shown in table 36:
table 3672% bronopol-thiacetone wettable powder various index detection details
As can be seen from Table 36, the 72% bronopol thiacetone wettable powder of the bactericidal composition meets various standards of products and is a qualified product.
Product formulation excipient example 9: 66% bronopol-bispyrithionic wettable powder (55:11)
The composition of 66% bronopol-bispyrithionic wettable powder is shown in table 37:
TABLE 3766% bronopol-bispyrithion wettable powder
According to the specification of each component in table 37, a carrier and an active ingredient 2 are mixed for 10min by a gravity-free mixer, the active ingredient 1, a wetting agent, a dispersing agent and a filler are sequentially added, after the mixing for 10min, the mixed material is subjected to airflow crushing, and the D98 is not more than 18 μm, so that 66% of the bactericidal composition 66% of the composition is prepared, and the product standard detection result is shown in table 38:
table 3866% bronopol-bispyrithium wettable powder each index detection detail
As can be seen from Table 38, 66% of the diniconazole/bispyribac wettable powder of the bactericidal composition meets all the standards of the product, and is a qualified product.
Product formulation excipient example 10: 66% bronopol-dipyrithione wettable powder (60:6)
The composition of 66% bronopol-bispyrithiobac wettable powder is shown in table 39:
TABLE 3966% wettable powder of bronopol-bispyrithium
According to the specification of each component in table 39, a carrier and an active ingredient 2 are mixed for 10min by a gravity-free mixer, the active ingredient 1, a wetting agent, a dispersing agent and a filler are sequentially added, after the mixing for 10min, the mixed material is subjected to airflow crushing, and the D98 is not more than 18 μm, so that 66% of the bactericidal composition 66% of the composition is prepared, and the product standard detection result is shown in table 40:
TABLE 4066% bronopol-bispyrithion wettable powder
As can be seen from Table 40, 66% of the bronopol-bispyrithium wettable powder of the bactericidal composition meets all the standards of the product, and is a qualified product.
According to the formulation excipient examples of the products, the inventor performs field efficacy test on the above formulations. The field efficacy standard of the product is described as follows:
the inventor verifies the control effect of the medicament of the embodiment of the invention on a large number of targets, and the part shows the control effect of the composition of the invention by taking part of preparation example products as examples for citrus canker.
The test refers to the 103 th part of the national standard GB/T17980.103-2004 pesticide field efficacy test criterion (II) of the people's republic of China: the bactericide is used for controlling citrus canker and is tested by the following steps:
1. the purpose of the test is as follows: the field control effect of different compound combined preparations on citrus canker is achieved;
2. the control object is: citrus canker of citrus (Xanthomonas Campestris pv. citri)
3. The application method and the water consumption are as follows: the water consumption per hectare by spraying method is 1125L (75L/667 square meter)
4. Cell arrangement, area and repetition:
the cell arrangement adopts a random block arrangement mode, is determined according to the site, and is repeated for 5 times, wherein 4 citrus trees are 1 cell.
5. The test investigation and calculation method comprises the following steps:
5.1, investigation time: survey on 29-30 days after the last application.
5.2, an investigation method:
according to the grading of the harmfulness symptom degree of the leaf sheath and the leaf blade of the citrus tree, 2 plants are randomly investigated in each cell, each plant is sampled according to 5 points in east-west, south-north, and each point investigates all the leaf blades on two tips.
Leaf (fruit) grading criteria:
level 0: no disease;
level 1: 1-5 scabs exist on each leaf (fruit);
and 3, level: each leaf (fruit) has 6-10 spots;
and 5, stage: each leaf (fruit) has 11-15 scabs;
and 7, stage: each leaf (fruit) has 16-20 scabs;
and 9, stage: each leaf (fruit) has more than 21 scabs.
5.3 method for calculating drug effect
Product field efficacy example 1: application of bronopol and matrine
The experiment is carried out in a Wuming area of Nanning, the total area of the fruit field is about 500 mu, the irrigation and drainage conditions are normal, and the management is normal. About 250 mu of woolly oranges generate citrus canker all the year round, and young fruits and spring shoots have serious diseases. The experimental fruit tree has good growth vigor and the planting specification is 2.0m multiplied by 4.0 m. The cultivation conditions (soil type, water and fertilizer management, transplanting period, planting density, growth period and water layer management) of all the test districts of the test orchard are relatively uniform and consistent. The application is spring shoot period, and no other bactericide is applied to the orchard test 20 days before and during the test period.
Based on the above conditions, the inventors designed that the spray was applied once every 5/4 days in 2020, 12/5/2020, and 20/5/2020, three times in total, based on table 41. The water consumption of the community is 3.6 liters, the water consumption of each plant is 0.9 liter, and the blank control is sprayed with clean water with the same amount.
Table 41 reagent test design
Designed according to table 41, the inventor counted the disease index and analyzed the prevention and treatment effect according to the above test investigation and calculation method at 18.6.2020, and the results are shown in table 42:
TABLE 42 test results for the control of citrus canker with each formulation
As can be seen from Table 42, the field control effect of the bronopol and matrine preparations with different proportions on citrus canker is better than that of the single preparation of the bronopol and the matrine. The dosage of the product preparation excipient example 1 is diluted by 800 times, the dosage of the effective components is 250mg/kg, the control effect is the best, and the average control effect reaches 84.97%.
Product field efficacy example 2: application of berberine and bronopol
The experiment is carried out in a Wuming area of Nanning, the total area of the fruit field is about 500 mu, the irrigation and drainage conditions are normal, and the management is normal. About 250 mu of woolly oranges generate citrus canker all the year round, and young fruits and spring shoots have serious diseases. The experimental fruit tree has good growth vigor and the planting specification is 2.0m multiplied by 4.0 m. The cultivation conditions (soil type, water and fertilizer management, transplanting period, planting density, growth period and water layer management) of all the test districts of the test orchard are relatively uniform and consistent. The application is spring shoot period, and no other bactericide is applied to the orchard test 20 days before and during the test period.
Based on the above conditions, the inventors designed that the spray was applied once every 5/4 days in 2020, 12/5/2020, and 20/5/2020, three times in total, according to table 43. The water consumption of the community is 3.6 liters, the water consumption of each plant is 0.9 liter, and the blank control is sprayed with clean water with the same amount.
Table 43 test design for test agent
According to the test design in table 43, the inventor makes statistics on disease index and analyzes the prevention and treatment effect according to the test investigation and calculation method on 6-18 th of 2020, and the results are shown in table 44:
TABLE 44 test results for the control of cucumber bacterial angular leaf spot with each formulation
As can be seen from Table 44, the field control effect of the bronopol and berberine preparations with different proportions on citrus canker is better than that of the bronopol and berberine single preparations. The dosage of the product preparation excipient example 3 is diluted by 3000 times, the dosage of the effective components is 200mg/kg, the control effect is the best, and the average control effect reaches 83.08%.
Product field efficacy example 3: application of bismerthiazol and bronopol
The experiment is carried out in a Wuming area of Nanning, the total area of the fruit field is about 500 mu, the irrigation and drainage conditions are normal, and the management is normal. About 250 mu of woolly oranges generate citrus canker all the year round, and young fruits and spring shoots have serious diseases. The experimental fruit tree has good growth vigor and the planting specification is 2.0m multiplied by 4.0 m. The cultivation conditions (soil type, water and fertilizer management, transplanting period, planting density, growth period and water layer management) of all the test districts of the test orchard are relatively uniform and consistent. The application is spring shoot period, and no other bactericide is applied to the orchard test 20 days before and during the test period.
Based on the above conditions, the inventors designed that the spray was applied once every 5/4 days in 2020, 12/5/2020, and 20/5/2020, three times in total, according to table 45. The water consumption of the community is 3.6 liters, the water consumption of each plant is 0.9 liter, and the blank control is sprayed with clean water with the same amount.
Table 45 test design for test agent
According to the test design of table 45, the inventor counted the disease index and converted the prevention and treatment effect according to the above test investigation and calculation method at 18/6/2020, and the results are shown in table 46:
TABLE 46 test results for the control of citrus canker with each formulation
As can be seen from Table 46, the field control effect of the bronopol and bisumuxer preparation products with different proportions on citrus canker is better than that of the bronopol and bisumuxer single preparation. The dosage of the excipient example 5 of the product preparation is 2640 times diluted, the dosage of the effective components is 250mg/kg with the best control effect, and the average control effect reaches 85.38%.
Product field efficacy example 4: application of thiacetone and bronopol
The experiment is carried out in a Wuming area of Nanning, the total area of the fruit field is about 500 mu, the irrigation and drainage conditions are normal, and the management is normal. About 250 mu of woolly oranges generate citrus canker all the year round, and young fruits and spring shoots have serious diseases. The experimental fruit tree has good growth vigor and the planting specification is 2.0m multiplied by 4.0 m. The cultivation conditions (soil type, water and fertilizer management, transplanting period, planting density, growth period and water layer management) of all the test districts of the test orchard are relatively uniform and consistent. The application is spring shoot period, and no other bactericide is applied to the orchard test 20 days before and during the test period.
Based on the above conditions, the inventors designed that the spray was applied once every 5/4 days in 2020, 12/5/2020, and 20/5/2020, three times in total, according to table 47. The water consumption of the community is 3.6 liters, the water consumption of each plant is 0.9 liter, and the blank control is sprayed with clean water with the same amount.
Table 47 test design of test agent
According to the test design in table 47, the inventor counted the disease index and converted the prevention and treatment effect according to the above test investigation and calculation method on 18 th 6 th 2021, and the results are shown in table 48:
TABLE 48 test results for the control of citrus canker with each formulation
As can be seen from Table 48, the field control effect of the bronitol and benziothiazolinone preparation products with different proportions on citrus canker is better than that of the bronitol and benziothiazolinone single dose. The dosage of the product preparation excipient 7 is 6000 times diluted, the dosage of the effective components is 120mg/kg, the control effect is the best, and the average control effect reaches 90.02%.
Product field efficacy example 5: application of bispyrithion and bronopol
The experiment is carried out in a Wuming area of Nanning, the total area of the fruit field is about 500 mu, the irrigation and drainage conditions are normal, and the management is normal. About 250 mu of woolly oranges generate citrus canker all the year round, and young fruits and spring shoots have serious diseases. The experimental fruit tree has good growth vigor and the planting specification is 2.0m multiplied by 4.0 m. The cultivation conditions (soil type, water and fertilizer management, transplanting period, planting density, growth period and water layer management) of all the test districts of the test orchard are relatively uniform and consistent. The application is spring shoot period, and no other bactericide is applied to the orchard test 20 days before and during the test period.
Based on the above conditions, the inventors designed that the spray was applied once every 5/4 days in 2020, 12/5/2020, and 20/5/2020, three times in total, according to table 49. The water consumption of the community is 3.6 liters, the water consumption of each plant is 0.9 liter, and the blank control is sprayed with clean water with the same amount.
Table 49 test design for test agents
According to the test design shown in table 49, the inventor makes statistics on disease index and analyzes the prevention and treatment effect according to the test investigation and calculation method at 6 month and 18 days in 2020, and the results are shown in table 50:
TABLE 50 test results for the control of citrus canker with each formulation
As can be seen from Table 50, the field control effect of the bronopol and the bispyrithione preparation products with different proportions on citrus canker is better than that of the bronopol and the bispyrithione single agent. The dosage of the excipient example 9 of the product preparation is 2640 times diluted, the dosage of the effective components is 250mg/kg with the best control effect, and the average control effect reaches 85.70%.
In conclusion, compared with the existing preparation, the bactericidal composition for preventing and treating crop bacterial diseases has an obvious synergistic effect and a good prevention and treatment effect, can slow down the generation of drug resistance of bacterial diseases, and is worthy of popularization and application in agricultural production.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. The bactericidal composition is characterized by comprising an alkaloid and alkaloid analogue component A and bromonitrol, wherein the alkaloid and alkaloid analogue component A is one selected from sophora flavescens extracts, berberine, biscumin, quinone or dipyrithione, the weight part ratio of the alkaloid and alkaloid analogue component A to the bromonitrol is 119: 1-1: 39, and the total weight of the alkaloid and alkaloid analogue component A and the bromonitrol accounts for 1-90% of the total weight of the bactericidal composition.
2. The bactericidal composition according to claim 1, further preferably wherein the weight ratio of the alkaloid and alkaloid analog component A to the bronopol is 99: 1-1: 19, and the total weight of the active component A and the bronopol accounts for 1% -80% of the total weight of the bactericidal composition.
3. The bactericidal composition of claim 1, wherein the bactericidal composition is formulated as wettable powder, soluble powder, water dispersible granule, soluble granule, aqueous emulsion, microemulsion, emulsifiable concentrate, suspension, dispersible oil suspension, microcapsule suspension, soluble solution, suspoemulsion, suspension-microcapsule suspension, aqueous emulsion-microcapsule suspension.
4. The bactericidal composition of claim 3, further comprising a common auxiliary agent required for preparing a pesticide preparation, wherein the common auxiliary agent is one or a mixture of several of a solvent, an emulsifier, a wetting agent, a stabilizer, a dispersant, a thickener, a pH regulator, an antifoaming agent, an antifreezing agent, a filler and the like.
5. Use of the fungicidal composition of claim 1 for controlling crop bacterial diseases.
6. Use of the fungicidal composition of claim 1 for the control of bacterial diseases in crops selected from the group consisting of food crops, commercial crops, fruit and vegetable and lawn garden crops.
7. Use of a fungicidal composition according to claim 1 for controlling crop bacterial diseases, which acts on phytopathogenic fungi and/or their environment or on plants, parts of plants, plant propagation material and subsequently growing plant organs, soil or cultivation media, materials or spaces.
8. The method of using the fungicidal composition of claim 1, characterized in that the fungicidal composition of claim 1 is applied in seed treatment, foliar application, stem application, drenching, drip, pour, spray, dusting, scattering or fuming of the plant, plant propagation material and plant organs, cultivation media, materials or spaces that grow out later in an agronomically effective and substantially non-phytotoxic application rate.
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