CN114847073A

CN114847073A - Method for improving sprouting capacity of Yunnan pine seedlings based on exogenous hormones

Info

Publication number: CN114847073A
Application number: CN202210604346.7A
Authority: CN
Inventors: 蔡年辉; 陈诗; 唐军荣; 陈林; 许玉兰
Original assignee: Southwest Forestry University
Current assignee: Southwest Forestry University
Priority date: 2022-05-31
Filing date: 2022-05-31
Publication date: 2022-08-05

Abstract

The invention discloses a method for improving the sprouting capacity of Yunnan pine seedlings based on exogenous hormones, which comprises the following steps: cutting off the top of Yunnan pine nursery stock, and spraying IAA and 6-BA water solution. The invention has the beneficial effects that: the method adopts annual Yunnan pine seedling to carry out the top cutting treatment, and sprays exogenous hormones with different concentrations after the top cutting, so that the growth number and the accumulated number of the sprouts can be increased, the highest sprout number is 27, the yield is improved by 36.5 percent compared with a reference, and the highest yield of the sprout growth amount of single exogenous hormone IAA is improved by 18.2 percent compared with the reference.

Description

Method for improving sprouting capacity of Yunnan pine seedlings based on exogenous hormones

Technical Field

The invention belongs to the technical field of seedling cultivation, and particularly relates to a method for improving the sprouting capacity of Yunnan pine seedlings based on exogenous hormones.

Background

Yunnan pine is a evergreen tall tree of Pinus of Pinaceae, is an important tree species in southwest, has the characteristics of fast growth, straight trunk, strong adaptability, drought resistance, barren and the like, and is one of main economic tree species and ecological tree species in a distribution area. Due to the influence of various reasons, the existing Yunnan pine forest stand has less excellent gene resources, and genetic improvement is imperative. Therefore, a breeding strategy of sexual creation and asexual propagation can be adopted in the genetic improvement process of the Yunnan pine, the obtained high-quality young panicle is the basis of asexual propagation, and practices prove that the germination of the panicle can be promoted by removing the top, and the proper exogenous hormone has important significance for the growth of plants. The method adopts a two-factor regression test design to perform top cutting on annual Yunnan pine seedlings in different seasons, compares the sprouting capacity and the seedling growth condition of the Yunnan pine seedlings sprayed with different exogenous hormones, has important application value on the acquisition of high-quality scions of the Yunnan pine, and can provide a solution for the management of a Yunnan pine cutting orchard and the development of clonal forestry of the Yunnan pine.

Disclosure of Invention

The invention aims to provide a method for improving the sprouting capacity of Yunnan pine seedlings based on exogenous hormones, which can effectively improve the number of sprouts of the Yunnan pine seedlings.

The technical scheme adopted by the invention is as follows:

a method for improving the sprouting capacity of Yunnan pine seedlings based on exogenous hormones comprises the following steps: cutting off the top of Yunnan pine nursery stock, mixing IAA water solution and 6-BA water solution to obtain mixed solution, and spraying.

Preferably, the IAA content in the mixed solution is 100-300 mg/L, and the 6-BA content is 100-200 mg/L.

Particularly preferably, the mixed solution has an IAA content of 200mg/L and a 6-BA content of 140 mg/L.

Preferably, the spraying frequency is 1-10 times; the spraying period is 1 time every 3-10 days.

Particularly preferably, the spraying times are 4 times; the spraying period is 1 time every 7 days.

Preferably, the Yunnan pine nursery stock is annual nursery stock.

Preferably, the height of the truncated top is 5-15 cm.

Particularly preferably, the height of the truncated vertex is 10 cm.

Preferably, field weeding, irrigation, water and fertilizer management, illumination and temperature management are also included.

Preferably, the seedling raising method of the Yunnan pine seedlings comprises the following steps: airing and air-drying the collected Yunnan pine cones, taking out seeds in the cones after the cones crack, sowing the seeds on a seedbed in a dibbling mode, selecting seedlings with basically consistent individual sizes after the seeds germinate, transplanting the seedlings, taking a nutrition pot as a pot culture container, and configuring a culture medium by humus soil and nursery soil according to the ratio of 2:1 to obtain annual Yunnan pine seedlings with more consistent growth.

The invention has the beneficial effects that:

the method adopts annual Yunnan pine seedling to carry out the top cutting treatment, and sprays exogenous hormones with different concentrations after the top cutting, so that the growth number and the accumulated number of the sprouts can be increased, the highest sprout number is 27, the yield is improved by 36.5 percent compared with a reference, and the highest yield of the sprout growth amount of single exogenous hormone IAA is improved by 18.2 percent compared with the reference.

Drawings

FIG. 1 is a reaction curve diagram of the number and yield of sprouts sprayed with different exogenous hormones;

FIG. 2 is a reaction curve diagram of the yield of spray sprouting growth of different exogenous hormones.

Detailed Description

In order to make the production process and technical effects of the present invention known in detail to those skilled in the art, the following is a specific production example to further describe the application and technical effects of the present invention.

Example 1

Collecting Yunnan pine cones, airing and air-drying, taking out seeds in the cones after the cones crack, sowing the seeds on a seedbed in a dibbling mode, selecting seedlings with basically consistent individual sizes after the seeds germinate, transplanting the seedlings, taking a nutrition pot as a pot container, and preparing a culture medium by using humus soil and nursery soil according to the ratio of 2: 1. The seedling management is the same as the conventional seedling management, and annual Yunnan pine potted seedlings with consistent growth are selected for experimental study.

And (4) cutting the annual Yunnan pine seedlings, wherein the cutting height is 10 cm. Spraying IAA and 6-BA aqueous solution according to the experimental design after the top is cut, spraying 1 time every 7 days, wherein the spraying amount is preferably no water dripping on the leaves, and applying 4 times in total. The using amount of the fertilizer is shown in table 1, and the seedlings in the later period are managed according to normal field weeding, irrigation and other management measures, and the water and fertilizer management, the illumination and the temperature are the same in the period.

TABLE 1 exogenous IAA and 6-BA dispensing test protocol

Treatment number	IAA(mg/L)	6-BA(mg/L)
			J1	0	0
J2	0	100
			J3	0	200
J4	150	0
			J5	150	100
J6	150	200
			J7	300	0
J8	300	100
			J9	300	200

Measuring the number of the tillers increased and the accumulated number of the tillers after the pruning, fitting according to the measuring results, and as shown in fig. 1 and 2, obtaining the highest yield according to fig. 1, namely the top point of a curved surface diagram, wherein the number of the highest tillers is 27, the yield is improved by 36.5% compared with a control (no exogenous hormone is sprayed), and the corresponding dosage of IAA and 6-BA are respectively: 200(mg/L) and 140 (mg/L). The highest yield of sprouting growth of single exogenous hormone IAA is improved by 18.2 percent compared with that of a control.

The number of sprouts is 20.51 when the concentration of the exogenous hormone IAA is 94 mg/L; the number of sprouts was 24.20 when exogenous hormone 6-BA was administered alone at a concentration of 108mg/L, 19.81 sprouts when exogenous hormone was not administered (control), 200mg/L IAA and 140 mg/L6-BA in combination with 27.05 sprouts. The spraying of different exogenous hormones has different influences on the number of sprouts, and the specific IAA aqueous solution and the 6-BA aqueous solution are mixed for use, so that the remarkable technical effect is achieved.

Finally, it should be noted that the above embodiments are only used for illustrating and not limiting the technical solutions of the present invention, and although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the present invention without departing from the spirit and scope of the present invention, and all modifications or partial substitutions should be covered by the scope of the claims of the present invention.

Claims

1. A method for improving the sprouting capacity of Yunnan pine seedlings based on exogenous hormones is characterized by comprising the following steps: cutting off the top of Yunnan pine nursery stock, mixing IAA water solution and 6-BA water solution to obtain mixed solution, and spraying.

2. The method for improving the tillering capacity of Yunnan pine seedlings based on the exogenous hormone as claimed in claim 1, wherein the content of IAA in the mixed solution is 100-300 mg/L, and the content of 6-BA in the mixed solution is 100-200 mg/L.

3. The method for improving the tillering capacity of Yunnan pine seedlings based on the exogenous hormone as claimed in claim 2, wherein the content of IAA in the mixed solution is 200mg/L, and the content of 6-BA in the mixed solution is 140 mg/L.

4. The method for improving the sprouting ability of the Yunnan pine seedlings based on the exogenous hormone according to any one of claims 1 to 3, wherein the spraying is performed for 1 to 10 times; the spraying period is 1 time every 3-10 days.

5. The method for improving the sprouting ability of Yunnan pine seedlings based on exogenous hormones as claimed in claim 4, wherein the spraying is performed for 4 times; the spraying period is 1 time every 7 days.

6. The method for improving the tillering capacity of Yunnan pine seedlings based on exogenous hormones as claimed in any one of claims 1 to 3, wherein the Yunnan pine seedlings are annual seedlings.

7. The method for improving the tillering capacity of Yunnan pine seedlings based on the exogenous hormones as claimed in any one of claims 1 to 3, wherein the height of the truncated top is 5-15 cm.

8. The method for improving the tillering capacity of Yunnan pine seedlings based on the exogenous hormones as claimed in claim 7, wherein the height of the truncated top is 10 cm.

9. The method for improving the tillering capacity of Yunnan pine seedlings based on the exogenous hormones as claimed in any one of claims 1 to 3, further comprising field weeding, irrigation, water and fertilizer management, illumination and temperature management.

10. The method for improving the tillering capacity of Yunnan pine seedlings based on exogenous hormones as claimed in any one of claims 1 to 3, wherein the method for growing the seedlings of the Yunnan pine comprises the following steps: airing and air-drying the collected Yunnan pine cones, taking out seeds in the cones after the cones crack, sowing the seeds on a seedbed in a dibbling mode, selecting seedlings with basically consistent individual sizes after the seeds germinate, transplanting the seedlings, taking a nutrition pot as a pot culture container, and configuring a culture medium by humus soil and nursery soil according to the ratio of 2:1 to obtain annual Yunnan pine seedlings with more consistent growth.