CN114631545A - Plant source rooting agent for promoting plant rooting and preparation method thereof - Google Patents
Plant source rooting agent for promoting plant rooting and preparation method thereof Download PDFInfo
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- 230000001737 promoting effect Effects 0.000 title claims description 23
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- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
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- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of growth promoters, and particularly discloses a strawberry rooting agent, a preparation method and application thereof, wherein the method comprises the following steps: (1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution; (2) and (2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, and uniformly stirring to obtain the plant-derived rooting agent. The rooting agent disclosed by the invention completely adopts environment-friendly plant source components on one hand, is very safe when coming from plants, and has very obvious growth promotion effect and obvious effect on the plants.
Description
Technical Field
The invention belongs to the technical field of plant growth regulators, and particularly relates to a plant source rooting agent for promoting plant rooting and a preparation method thereof.
Background
The rooting agent is used as the matter for regulating plant growth and development and has the main components of naphthylacetic acid, gibberellin, indoleacetic acid, etc. The rooting agent sold in the market comprises water agent and powder. The rooting agent is irrigated to the planted trees, so that the generation of new roots of the trees can be accelerated, and the growth of the trees is promoted. The rooting agents on the market can be mainly divided into the following three categories: common rooting and seedling strengthening agents (single compounds such as indoleacetic acid, sodium naphthaleneacetate and the like or a mixture of the compounds according to a scientific proportion), a compound rooting agent formed by mixing biological bacteria and the rooting agent, and rooting agents formed by compounding nutrient elements and growth promoter substances (such as root tuber and the like).
For example, patent 201911148328.7 discloses a vitis heyneana rooting agent and a vitis heyneana cutting seedling raising method, wherein the rooting agent comprises the following raw material components: 200mg/L of naphthylacetic acid, 5-10mg/L of 5-methyl-3-p-chlorophenyl-2-thiohydantoin, 35-50mg/L of proline, 5-15mg/L of bamboo vinegar and 50-80mg/L of ammonium bicarbonate. The grape rooting agent can effectively promote the root growth of the cuttage branches of the downy grapes, and the cuttage seedling culture method further effectively improves the rooting amount and the survival rate of the cuttage branches of the downy grapes by combining the ventilation and water-passing device and the rooting agent. Patent 201910779427.9 discloses a microorganism rooting agent and a preparation method thereof, wherein the rooting agent comprises the following raw materials in parts by weight: 1-10 parts of macromolecular bamboo fiber, 0.1-5 parts of bacillus subtilis, 0.1-5 parts of azotobacter vinelandii, 0.1-5 parts of bacillus mucilaginosus and 20-200 parts of bamboo fiber; the rooting agent provides a stable and rapid growth environment for microorganisms around the plant rhizosphere through the moisture provided by the macromolecular bamboo fibers, the carbon source provided by the bamboo fibers and the nitrogen source provided by the microbial bacteria, the microorganisms rapidly propagate, and metabolites of the microorganisms generate plant growth hormones such as indoleacetic acid, gibberellin and polypeptide, so that the growth of the plant root system is promoted. In addition, the applicant of the invention also develops a plant source rooting agent and a preparation method thereof (patent 202010668150.5), the rooting agent adopts willow extract, Chinese violet extract and chlorophytum comosum extract as effective components of the rooting agent, can effectively promote the rooting of crops and improve the waterlogging resistance of the crops, and the effective components are plant extracts, so that the rooting agent is green and safe and has high environmental protection performance. However, the rooting agent still has the defect of insufficient rooting promoting effect, and the survival rate and the rooting rate need to be further improved.
Disclosure of Invention
The invention aims to provide a plant source rooting agent for promoting plant rooting and a preparation method thereof.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
preferably, the plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
further preferably, the plant source rooting agent for promoting plant rooting is characterized by being prepared from the following raw materials in parts by weight:
preferably, the preparation method of willow extract is the same as patent 202010668150.5, and the specific steps are as follows:
(1) drying willow bark and willow branch, heating to 70-80 deg.C, maintaining for 3-8min, rapidly soaking in liquid nitrogen, and maintaining for 30-40 min;
(2) crushing the material treated in the step (1), and sieving the crushed material with a 70-100-mesh sieve to obtain a willow crushed material; adding water 7-10 times the weight of the crushed willow material, adding pectinase and neutral cellulase, wherein the addition amount of the pectinase is 0.5-1% of the weight of the crushed willow material, and the addition amount of the neutral cellulase is 2-3.5% of the weight of the crushed willow material; raising the temperature of the system to 50-60 ℃, stirring, carrying out enzymolysis for 2-3h, and then carrying out enzyme deactivation treatment at 100 ℃;
(3) adding ethanol with the mass 5-8 times of the crushed willow material into the system treated in the step (2), and performing microwave extraction while stirring, wherein the stirring speed is 200-500r/min, the microwave power is 100-300W, and the extraction time is 30-50 min; centrifuging, collecting supernatant, and filtering with filter membrane to obtain extractive solution; concentrating the extract to obtain a concentrated solution; drying the concentrated solution at 60-70 deg.C to constant weight to obtain willow extract.
Preferably, the preparation method of the shiitake mushroom extract comprises the following steps: cleaning 50-100 parts of fresh mushrooms with deionized water, adding the cleaned fresh mushrooms into 1000 parts of 60 ℃ deionized water, soaking for 2-4h, stirring the mushrooms by using a homogenizer, treating the stirred mushrooms by using an ultrasonic cell disruptor, filtering a mushroom extracting solution by using gauze and a nylon filter screen in sequence after treatment to remove large granular substances, soaking filter residues in deionized water with the volume of 3 times for 1-2h, performing ultrasonic treatment again, combining filtrates obtained after filtration, centrifuging to remove precipitates, and decocting the filtrate for 30-60min for concentration to obtain the mushroom extract.
Preferably, the preparation method of the blackberry extract comprises the following steps: placing the clean blackberries into a homogenizer, adding water with the mass of 4-8 times of that of the blackberries for homogenizing extraction, then filtering to obtain filtrate, placing the filtrate into an ultrasonic device for ultrasonic extraction, placing the filtrate after ultrasonic extraction for a period of time, and centrifuging, wherein the filtrate after centrifugation is the blackberry extract.
Preferably, the seaweed extract contains 21.5g/L alginic acid and more than 24% of seaweed polysaccharide, polyphenol, betaine, polyamine and the like.
Preferably, the preparation method of the jujube leaf extract comprises the following specific steps: firstly, cleaning, drying and crushing jujube leaves into powder, then adding an ethanol solution with the mass being 4-8 times of that of the jujube leaves into the jujube leaves powder for ultrasonic extraction, then filtering to obtain a filtrate, and concentrating the filtrate to remove ethanol to obtain the jujube leaf extract.
Preferably, the birch sap is collected from mature birch forests without pollution and diseases and insect pests and birch trees with the diameter at breast height of more than 18cm, a hard plastic tube liquid collecting tube is adopted, when the liquid collecting tube is installed, one end of the tube is gently driven into a drilled tree hole by a small hammer, and the joint is tightly inserted and connected and is leakproof. The other end of the liquid collecting tube is connected with a liquid conveying tube, and the other end of the liquid conveying tube is connected with a liquid collecting barrel or a liquid collecting bag. The liquid collecting bag is fixed by a rope to prevent the juice from falling out of the bag due to wind. The storage temperature of the collected birch sap is 2-7 ℃.
The invention also claims a preparation method of the plant source rooting agent, which comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) and (2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, and uniformly stirring to obtain the plant-derived rooting agent.
Preferably, in the step (2), the mass ratio of water to ethanol to fatty alcohol-polyoxyethylene ether to xanthan gum to glycerol is 100: 20: 2: 0.4: 3.
compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the components of the plant source rooting agent are all from plants, and the plant source rooting agent is safe and environment-friendly;
(2) the plant source rooting agent of the invention consists of 7 components of willow extract, mushroom extract, blackberry extract, seaweed extract, jujube leaf extract, bamboo vinegar and birch sap, can generate synergistic effect, is beneficial to survival and rooting of plants, the willow extract and the birch liquid contain rich salicylic acid and mineral components, the lentinan extract contains rich lentinan, the seaweed extract contains multiple nutrient substances such as alginic acid and seaweed polysaccharide, the alginic acid and the seaweed polysaccharide are beneficial to survival and root growth of plants, the blackberry extract can accelerate germination of capillary roots and cell division, the jujube leaf extract contains triterpenes for promoting growth, and the bamboo vinegar liquid contains multiple organic components such as organic acid, phenols, ketones, aldehydes, alcohols and heterocycles, so that the effects of sterilizing, disinfecting and regulating plant growth can be achieved, and good growth promoting and rooting effects can be achieved.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following examples, the seaweed extract was obtained from Xin Biotech, Inc. in Shaanxi and the bamboo vinegar solution was obtained from bamboo vinegar, Biotech, Inc. of Guangdong sea Peng.
Example 1
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 30min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
the preparation method of the willow extract comprises the following specific steps:
(1) drying willow bark and willow branch, heating to 75 deg.C, maintaining for 6min, and rapidly soaking in liquid nitrogen for 35 min;
(2) crushing the material treated in the step (1), and sieving the crushed material with a 100-mesh sieve to obtain a willow crushed material; adding water with the mass 8 times of that of the willow crushed material into the willow crushed material, and adding pectinase and neutral cellulase, wherein the addition amount of the pectinase is 0.6 percent of the mass of the willow crushed material, and the addition amount of the neutral cellulase is 3 percent of the mass of the willow crushed material; raising the temperature of the system to 55 ℃, stirring, carrying out enzymolysis for 2.5h, and then carrying out enzyme deactivation treatment at 100 ℃;
(3) adding ethanol with the mass 6 times of that of the willow crushed materials into the system treated in the step (2), and performing microwave extraction while stirring at the stirring speed of 400r/min and the microwave power of 200W for 50 min; centrifuging, collecting supernatant, and filtering with filter membrane to obtain extractive solution; concentrating the extract to obtain a concentrated solution; then drying the concentrated solution at 65 deg.C to constant weight to obtain willow extract.
The preparation method of the mushroom extract comprises the following steps: cleaning 70 parts of fresh mushrooms with deionized water, adding the cleaned fresh mushrooms into 700 parts of 60 ℃ deionized water, soaking for 3 hours, stirring the mushrooms by using a homogenizer, treating the stirred mushrooms by using an ultrasonic cell disruptor, filtering a mushroom extracting solution by using gauze and a nylon filter screen in sequence after treatment to remove large-particle substances, soaking filter residues in 3 times of volume of deionized water for 2 hours, performing ultrasonic treatment again, combining filtrates obtained in two times after filtration, centrifuging to remove precipitates, and decocting the filtrate for 45 minutes to concentrate to obtain the mushroom extract.
The preparation method of the blackberry extract comprises the following steps: putting 50 parts of cleaned blackberry into a homogenizer, adding water with the mass 6 times of that of the blackberry for homogenizing extraction, rotating the homogenizer at the speed of 15000r/min and with the homogenizing power of 150W for 45min, then filtering to obtain filtrate, putting the filtrate into an ultrasonic device for ultrasonic extraction at the ultrasonic temperature of 45 ℃, the ultrasonic frequency of 100KHz and the ultrasonic power of 150W for 45min, standing the filtrate after ultrasonic extraction for a period of time, and centrifuging, wherein the filtrate after centrifugation is the blackberry extract.
The preparation method of the jujube leaf extract comprises the following specific steps: firstly, 50 parts of jujube leaves are cleaned, dried and crushed into powder, then 5 times of ethanol solution with the mass percent concentration of 65% is added into the jujube leaf powder to carry out ultrasonic extraction for 30min at the temperature of 55 ℃, then the filtrate is obtained by filtration, and the filtrate is concentrated to remove the ethanol, thus obtaining the jujube leaf extract.
The preparation methods of willow extract, shiitake mushroom extract, blackberry extract and jujube leaf extract in the following examples 2-5 and comparative examples 1-2 are the same as in example 1.
Example 2
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 20min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
example 3
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 25min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
example 4
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 30min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
example 5
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 20min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
comparative example 1
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, and folium Jujubae extract;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 30min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
comparative example 2
A plant source rooting agent for promoting plant rooting is prepared from the following raw materials in parts by weight:
the preparation method of the plant source rooting agent comprises the following steps:
(1) mixing willow extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch sap uniformly;
(2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, stirring for 30min, and uniformly mixing to obtain the plant source rooting agent; wherein the mass ratio of the water to the ethanol to the fatty alcohol-polyoxyethylene ether to the xanthan gum to the glycerol is 100: 20: 2: 0.4: 3.
the performance evaluation of the rooting agent prepared in examples 1, 3 and 5 and comparative examples 1-2 was carried out as follows:
totally designing 6 rooting agent treatment groups, wherein the rooting agents are respectively as follows:
treatment group 1: adding 100 times of water into 1g of the plant source rooting agent in the embodiment 1, and uniformly mixing to obtain a rooting agent solution;
treatment group 2: in the embodiment 3, 100 times of water is added into 1g of the plant source rooting agent, and the mixture is uniformly mixed to obtain a rooting agent solution;
treatment group 3: in example 5, 100 times of water was added to 1g of the plant-derived rooting agent, and the mixture was mixed uniformly to obtain a rooting agent solution.
Treatment group 4: and (3) adding 100 times of water into 1g of the plant source rooting agent in the comparative example 1, and uniformly mixing to obtain a rooting agent solution.
Treatment group 5: and (3) adding 100 times of water into 1g of the plant-derived rooting agent in the comparative example 2, and uniformly mixing to obtain a rooting agent solution.
Selecting gardenia branches with the diameter of 0.3-0.6cm, normal growth and no plant diseases and insect pests as cuttings, wherein the number of the cuttings is 600, the cuttings are averagely divided into 6 groups, and each group comprises 100 cuttings.
(1) Moisturizing the cuttings: cutting the gardenia cutting slips into 10-13cm long before cutting, reserving 2-4 buds, cutting the upper end of each gardenia cutting slip 1cm away from the top bud, cutting the lower end of each gardenia cutting slip into an inclined plane, and then immersing the base parts of the cutting slips into clear water for later use;
(2) cuttage: respectively soaking 3cm base parts of 5 groups of gardenia cutting strips in the plant source rooting agent solution in the 5 groups of treatment groups, soaking 3cm base parts of 6 th group of cutting strips in clear water, and soaking the 6 th group of cutting strips in a control group for 20 min;
(3) inserting the treated cuttings into loose and breathable matrix prepared by mixing fine sand and coconut coir soil in a greenhouse, watering every day after cuttage, keeping the humidity in the greenhouse at about 70%, controlling the temperature in the greenhouse at about 28 ℃, and performing conventional management;
(4) recording the survival rate of the cuttings 50 days after cuttage, and calculating the average number of roots and the average root length of the surviving cuttings, wherein the specific results are shown in table 1.
Survival rate/% | Rooting percentage/%) | Average number of roots/strip | Average root length/mm | |
Treatment group 1 | 96 | 94 | 4.6 | 7.8 |
Treatment group 2 | 92 | 91 | 4.1 | 7.5 |
Treatment group 3 | 95 | 94 | 4.4 | 7.2 |
Treatment group 4 | 82 | 80 | 2.5 | 6.5 |
Treatment group 5 | 87 | 83 | 1.9 | 6.8 |
Control group | 51 | 43 | 1.3 | 4.2 |
Wherein, the method for calculating the survival rate of each group comprises the following steps: the number of survived cutting branches/the total number of cutting branches is 100 percent;
the rooting rate calculation method comprises the following steps: the number of rooted cutting branches/total number of cutting branches is 100%;
the average root number calculation method comprises the following steps: the total number of roots of the rooting cuttage branches/the number of the rooting cuttage branches;
the average root length calculation method comprises the following steps: the total root length/the number of rooting of the cuttage branches;
the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
4. the plant-derived rooting agent for promoting plant rooting according to any one of claims 1 to 3, wherein the shiitake mushroom extract is prepared by the method comprising: cleaning 50-100 parts of fresh mushrooms with deionized water, adding the cleaned fresh mushrooms into 1000 parts of 60 ℃ deionized water, soaking for 2-4h, stirring the mushrooms by using a homogenizer, treating the stirred mushrooms by using an ultrasonic cell disruptor, filtering a mushroom extracting solution by using gauze and a nylon filter screen in sequence after treatment to remove large granular substances, soaking filter residues in deionized water with the volume of 3 times for 1-2h, performing ultrasonic treatment again, combining filtrates obtained after filtration, centrifuging to remove precipitates, and decocting the filtrate for 30-60min for concentration to obtain the mushroom extract.
5. The plant-derived rooting agent for promoting plant rooting according to any one of claims 1 to 3, wherein the preparation method of the blackberry extract comprises the following steps: placing the clean blackberries into a homogenizer, adding water with the mass of 4-8 times of that of the blackberries for homogenizing extraction, then filtering to obtain filtrate, placing the filtrate into an ultrasonic device for ultrasonic extraction, placing the filtrate after ultrasonic extraction for a period of time, and centrifuging, wherein the filtrate after centrifugation is the blackberry extract.
6. The plant-derived rooting agent for promoting plant rooting according to any one of claims 1 to 3, wherein the preparation method of the jujube leaf extract comprises the following specific steps: firstly, cleaning, drying and crushing jujube leaves into powder, then adding an ethanol solution with the mass being 4-8 times of that of the jujube leaves into the jujube leaves powder for ultrasonic extraction, then filtering to obtain a filtrate, and concentrating the filtrate to remove ethanol to obtain the jujube leaf extract.
7. The method for preparing the plant-derived rooting agent according to any one of claims 1 to 6, comprising the steps of:
(1) mixing willow extract, Lentinus Edodes extract, blackberry extract, Sargassum extract, folium Jujubae extract, bamboo vinegar solution and birch solution;
(2) and (2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the mixed solution obtained in the step (1), then adding water, and uniformly stirring to obtain the plant-derived rooting agent.
8. The preparation method according to claim 7, wherein the mass ratio of water, ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol in the step (2) is 100: 20: 2: 0.4: 3.
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