CN102986444B - Method for enabling woody plant material to form adventitious root and grow into own-rooted seedling - Google Patents
Method for enabling woody plant material to form adventitious root and grow into own-rooted seedling Download PDFInfo
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- Y02P60/216—
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a method for enabling a woody plant material to form an adventitious root and grow into an own-rooted seedling. The method includes selecting a full annual lignified branch or a cryptophytic perennial branch of the woody plant, maintaining the humidity needed for the survival of the branch, keeping germinal shoots etiolation and delaying lignification through controlling light, and inducing lateral twigs to root. The method is convenient to operate, low-carbon, environment-friendly, low in cost, and capable of enabling tree species difficult to obtain own-rooted seedlings through existing methods to obtain own-rooted seedlings easily and increasing the material selecting range of own-rooted seedling of tree species easy to cut.
Description
Technical field
The present invention relates to a kind of method of impelling the woody plant material to form adventive root and cultivating into own-rooted tree, relate in particular to a kind of method of impelling woody plant material that difficulty is taken root to form adventive root and cultivating into own-rooted tree, belong to woody plant propagation technique field.
Background technology
The superiority that own-rooted tree has grafting and seedling to replace in production and scientific research, not only have the advantage that keeps maternal good genetic character, and good uniformity arranged.
At present, the method for woody plant production own-rooted tree mainly contains cuttage, press strip, plant division and tissue and cultivates, and former three is wide-spread, ancient conventional method, uses till today always, and latter is the new method just occurred in 20th century.General cuttage, press strip and plant division are easy and simple to handle, do not need special installation, and simple and easy to do, expense is lower, but only are suitable for easily producing the plant of adventive root; The more difficult plant taken root generally adopt the band sectional leaf cutting of semi-lignified or claim green branch appropriateness shade or full exposure atomizing condition under cuttage obtain own-rooted tree, difficulty and expense all increase to some extent; More difficult take root will be by tissue culture technique, need strict sterile working, equipment needed thereby condition and staff condition are all higher, expense is also higher; Some woody plants are more arranged, there is no at present the ripe method that effectively produces own-rooted tree.
The ability that the vegetable material that degree of lignification is lower forms adventive root is stronger, but not lignified woody plant material leaves parent, is difficult to survive later, and just because of this, the more difficult plant taken root adopts semi-lignified cutting cuttage usually, or passes through tissue culture propagation.In the semi-lignified cutting, stored nutrient is less, and Chang Buneng meets the nutrient consumption in the branch rooting process, needs retain appropriate blade and be under solar radiation, by self photosynthesis manufacture nutrient, maintains the nutritional need in rooting process.But because there is no to absorb the root system of moisture, thereby transplant a cutting, expose and just face in the sun the withered danger of loss of water for a long time, therefore must reduce moisture loss by suitable spraying, the water balance that maintenance is transplanted a cutting.This makes semi-lignified cuttage process more complicated than hard cuttage, and conditional request is also higher.The common degree of lignification of the explant that tissue culture adopts is lower, need to, from outside, in medium, draw various nutriments.Nutritious in medium, also for the breeding of microorganism, created desirable condition, therefore must microorganism be isolated in outside culture environment by sterile working.Aseptic condition is the main cause that tissue culture propagation is loaded down with trivial details, cost is high.
Up to now, the characteristics that can utilize low degree of lignification branch easily to take root, do not need again higher environmental condition, inexpensive method easy and simple to handle to have not yet to see report.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of easy new method of obtaining the woody plant own-rooted tree.
Adopt following technical scheme for solving this technology:
(1) choose and enrich full annual lignified branch, or the perennial branch of the woody plant that kryptoblast is arranged, the stem section that is cut into length 10～20cm is placed in the plastics plastic shopping bag as cutting, use the liver moss moisturizing of soaking in bag, and to keep, in bag, 2/3 space to be arranged be air, keep in Dark Place, jede Woche is opened the plastics plastic shopping bag and is checked and breathe freely once, spraying and moisturizing;
(2) the late March lateral bud starts to sprout successively.Lateral bud or kryptoblast are laid in the stem section of preservation on draining ventilative good chesson, liver moss or other mixed-matrixes before and after sprouting, and continue moisturizing, lucifuge.At the beginning of 4 months, tender tip length reaches 3～5cm, now starts to penetrate faint scattered light;
(3) remove too much shoot, only retain 2 tender tips of new life on every root cutting
(4) high-voltage electrostatic field (HVEF) processes: intensity is 45KV/cm, time 2 h;
(5) root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, ABT0.5 part, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, 0.5 part of vitamin B1, with the dilution ratio of 600mg: 1L, soak cutting bottom 2h;
(6) will take root and transplant a cutting move in flowerpot, the matrix of flowerpot was preferably: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, described matrix mixes the thick 10cm of reaching of place mat, and cover newborn adventive root and take root tender tip base portion by matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks started to be exposed under natural sunlight after transplanting, water as required.
The beneficial effect of the relative prior art of the present invention is:
The application's method takes full advantage of the characteristics that the lignification branch is not easily taken root and has stored nutrient to perennial lignification branch in 1 year and also can, for the tender tip of new life provides the characteristics of nutrient continually, thereby make, generate adventive root incubation own-rooted tree.Main advantage has:
1 plant growth regulator is a kind of ectogenic non-nutritive chemical substance, it can conduct to site of action usually in plant corpus, just can promote or suppress some link in the plant life process with lower concentration, make it to the wish development that meets the human needs, plant establishment agent traditional in prior art is very limited for the rooting effect that promotes plant, especially for some plant with difficult rooting, such as ornamental trees and shrubs etc., effect is not remarkable, the present inventor draws root-inducing powder formula of the present invention by lot of experiments, it is easy to use, can improve improving activity of root system, promote root growth, improve the chlorophyll content of transplanting plant, strengthen photosynthesis, and the auxin rational proportion contained, Coordinated Interaction, there is the functions such as the root hair cell of promotion division, effectively improve growing of root hair, extend the suitable phase of plant transplantation, improve and transplant rear new root number and the long speed of new root, obviously shorten seedling-slowing stage, tachyauxesis.
For checking root-growing agent effect of the present invention, the woody plant apocarya of take is established following three processing as research object:
Process 1: commercially available powerful root-inducing powder; Step is with embodiment 1
Process 2: commercially available ABT root-inducing powder soaks; Step is with embodiment 1
Process 3: root-inducing powder of the present invention soaks, according to the processing mode of embodiment 1.
Table 1: the impact of root-inducing powder on root length, radical growth rate and survival rate
| ? | The long growth rate of root | The radical growth rate | Survival rate |
| Process 1 | 9.4％ | 10％ | 49％ |
| Process 2 | 19.5％ | 13.7％ | 61％ |
| Process 3 | 26.3％ | 21.5％ | 75％ |
2 utilize high-voltage electrostatic field to process the cuttage branch, and the motion that it has affected permeability and other polar molecule of film has improved absorption and the transhipment physical capacity of cell, thereby can promote the growth of tissue.It not only can strengthen improving activity of root system, increase the biomass of root, improve cuttage survival rate, promote growth of seedling, but also can strengthen premunition, kipeming high yield, yet, utilize high-voltage electrostatic field to process the cuttage plastid, different kind numerical value differ larger, different intensity and ageing can produce different effects, if it is excessive to process electric-field intensity, or the processing time is long, can make plant cell come to harm even dead, and electric-field intensity is too small or the time is too short, can cause again treatment effect not obvious, the present inventor show that through creative work adopting intensity for the apocarya branch is 45KV/cm, the treatment effect of time 2 h is best, the root growth rate, cuttage survival rate, vitality index numerical value maximum, conductivity values is minimum, every physical signs is extreme value.
Table 2: the impact of single-factor experimental verification high-voltage electrostatic field on the apocarya shoot growth
3 Screening matrix embodiment
Horse liver soil is the distinctive a kind of soil in THE LOWER YANGTZE VALLEY area, and Sha Xingqiang, do and can consolidate root, but wet water conservation, its shortcoming is that water permeable ability is strong, and dehydration is too fast, easily air-dry, and single use can make the base portion dehydration withered.Peat soil has good permeability and larger salt balance ability, perlite and vermiculite water suction, ventilative, the present inventor is through creative work for many years, screened the contrast experiment, from a large amount of cutting mediums, screen the liver soil of going into action, vermiculite, peat, these four kinds of matrix of perlite according to 3: 1: 2: 1 part by weight is combined with, do mutually mutual induction between each matrix, reach the effect of ventilative, moisturizing, solid root.
The invention discloses the method that some are difficult to according to a conventional method to obtain the woody plant material formation adventive root of seeds and are bred as own-rooted tree, greatly increased the scope of selecting material that conventional method can obtain the seeds cottage propagation of own-rooted tree, be not limited only to semi-lignified branch and annotinous branch, even can, by perennial tree section, the obsolete material of pruning be fully utilized.
Embodiment
Embodiment mono-
Choose 20 of the stem sections that Juglandaceae cultivated plant apocarya (Carya illinoensis) dormant wood is cut into length 20cm, diameter 10～15mm on January 25th, 2011, as cutting, be placed in the plastics plastic shopping bag, the liver moss moisturizing of soaking in bag, and to keep, in bag, 2/3 space to be arranged be air, keep in Dark Place.The late March lateral bud starts to sprout successively.At the beginning of 4 months, tender tip length reaches 3～5cm, now starts to penetrate faint scattered light; Remove too much shoot, only retain 2 tender tips of new life on every root cutting.April 26, high-voltage electrostatic field (HVEF) processes: intensity is 45KV/cm, time 2 h; Root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, 0.5 part of ABT7 root-inducing powder, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, 0.5 part of vitamin B1, with the dilution ratio of 600mg: 1L, soak cutting bottom 2h; To take root for transplanting a cutting and move in flowerpot, flowerpot matrix was: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, matrix mixes the thick 10cm of reaching of place mat, and cover newborn adventive root and take root tender tip base portion by matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks started to be exposed under natural sunlight after transplanting, water as required.
Had not yet to see that the tender tip that the cutting of taking root sends out rots afterwards successively on May 7 or withered.December 31 stopped, seedling 15 strains, and total planting percent reaches 75%, average height of seedling 29.4cm, base diameter average out to 0.97cm.
Embodiment bis-
On February 7th, 2012, choose rose family wild plant rubus coreanus (Rubus coreanus) stolon, be cut into 50 of the long stem sections of 15cm, as cutting, be placed in the plastics plastic shopping bag, the liver moss moisturizing of soaking in bag, and to keep, in bag, 2/3 space is arranged be air, keeps in Dark Place.Jede Woche is opened the plastics plastic shopping bag and is checked and breathe freely once, spraying and moisturizing, and the late March lateral bud starts to sprout successively.Treat that tender tip length reaches 3cm, now starts to penetrate faint scattered light; Remove too much shoot, only retain 2 tender tips of new life on every root cutting.Process high-voltage electrostatic field on April 3 (HVEF): intensity is 45KV/cm, time 2 h; Root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, 0.5 part of ABT7 root-inducing powder, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, vitaminB10 .5 part, with the dilution ratio of 600mg: 1L, soak cutting bottom 2h; To take root for transplanting a cutting and move in flowerpot, flowerpot matrix was: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3: 1: 2: 1, matrix mixes the thick 10cm of reaching of place mat, and cover newborn adventive root and take root tender tip base portion by matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks started to be exposed under natural sunlight after transplanting, water as required.Daily inspection in Mays 8, be shown in that 8 strains do not wither because taking root, 42 survivals.Still have by the end of November 42 strains to survive, survival rate reaches 84%.
Although, above the present invention is described in detail with a general description of the specific embodiments, but, on basis of the present invention, can make some modifications or improvements it, or the enforcement of the seeds outside case study on implementation this method, this will be apparent to those skilled in the art.Therefore, these that made are without departing from theon the basis of the spirit of the present invention revised, and the expansion of improvement or scope, all belong to the scope of protection of present invention.
Claims (1)
1. the method impelling the woody plant material to form adventive root and be bred as own-rooted tree is characterized in that:
(1) choose lignified annual extremely perennial branch 1-2 month, be cut into the stem section of length 15-20cm, as cutting, be placed in the plastics plastic shopping bag, use the liver moss moisturizing of soaking in bag, and to keep, in bag, 2/3 space to be arranged be air, keep in Dark Place, jede Woche is opened the plastics plastic shopping bag and is checked and breathe freely once, spraying and moisturizing;
(2) the late March lateral bud starts to sprout successively, and at the beginning of 4 months, tender tip length reaches 3～5cm, now starts to penetrate faint scattered light;
(3) remove too much shoot, only retain 2 tender tips of new life on every root cutting;
(4) high-voltage electrostatic field (HVEF) processes: intensity is 45KV/cm, time 2 h;
(5) root-inducing powder soaks, and described root-inducing powder is: 2 parts of α-naphthaleneacetic acid, 1 part of indolebutyric acid, ABT0.5 part, 5 parts of dipotassium tetraborates, 3 parts of potassium dihydrogen phosphates, vitamin B1 are 0.5 part; With the 600mg root-inducing powder: the dilution proportion of 1L water, then soak and transplant a cutting bottom 2h;
(6) will take root and transplant a cutting move in flowerpot, the matrix of flowerpot is: according to horse liver soil, vermiculite, peat soil and perlite weight ratio=3:1:2:1, described matrix mixes the thick 10cm of reaching of place mat, and cover newborn adventive root by matrix, cladding thickness reaches 3cm, increase gradually the scattering light intensity after moving into flowerpot, 2 weeks started to be exposed under natural sunlight after transplanting, water as required;
Described woody plant material is apocarya.
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| CN103238492B (en) * | 2013-05-13 | 2014-04-16 | 江苏省中国科学院植物研究所 | Process for facilitating sprouting of adventitious roots of apocarya branches |
| CN103299812B (en) * | 2013-06-20 | 2014-04-16 | 南京绿宙薄壳山核桃科技有限公司 | Apocarya hardwood cutting seedling raising method |
| CN104904499A (en) * | 2014-05-09 | 2015-09-16 | 常州市金土地农牧科技服务有限公司 | Method for grafting caryaillinoinensis |
| CN104396656B (en) * | 2014-09-23 | 2018-02-16 | 常州市金土地农牧科技服务有限公司 | A kind of high yield method of apocarya |
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