CN111849706A - Microbial cultivation device - Google Patents

Microbial cultivation device Download PDF

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Publication number
CN111849706A
CN111849706A CN202010760617.9A CN202010760617A CN111849706A CN 111849706 A CN111849706 A CN 111849706A CN 202010760617 A CN202010760617 A CN 202010760617A CN 111849706 A CN111849706 A CN 111849706A
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CN
China
Prior art keywords
microorganism culture
fixedly connected
rotating shaft
sliding
bevel gear
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Withdrawn
Application number
CN202010760617.9A
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Chinese (zh)
Inventor
刘滨璐
于伟红
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Qingdao Textile Supervision And Inspection Institute
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Qingdao Textile Supervision And Inspection Institute
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Priority to CN202010760617.9A priority Critical patent/CN111849706A/en
Publication of CN111849706A publication Critical patent/CN111849706A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of microorganism culture, and discloses a microorganism culture device which comprises a fixed frame, wherein a first fixed plate is arranged in the middle of the lower surface of the fixed frame, the middle of the first fixed plate is rotatably connected with a first rotating shaft, a driving motor is arranged on the right side of the first fixed plate, an output shaft of the driving motor is fixedly connected with a first bevel gear, the first bevel gear is meshed with a second bevel gear, the second bevel gear is fixedly connected with the lower end of the first rotating shaft, the upper end of the first rotating shaft is fixedly connected with a driving gear, the side wall of the driving gear is meshed with a driven gear, the upper part of the driven gear is provided with a sliding sleeve, the upper part of the sliding. The invention is suitable for a microorganism culture device, and in the process of moving the support plate left and right, the left and right ends of the support plate and the wedge block slide to enable the left and right ends of the support plate to tilt up and down, so that the movement of the conical flask is smoother, and the microorganism culture effect is improved.

Description

Microbial cultivation device
Technical Field
The invention relates to the technical field of microbial culture, in particular to a microbial culture device.
Background
The microbial culture refers to the rapid growth and propagation of some (kinds of) microorganisms by means of artificially prepared culture media and artificially created culture conditions (such as culture temperature, and the like), and is called microbial culture. The microbial culture can be divided into pure culture and mixed culture, wherein the pure culture refers to the culture and utilization of a single purified strain; the latter means that microorganisms in mixed strains or natural samples (such as soil) are cultured, and then the diversity and the quantity of the microorganisms in the soil can be estimated to a certain extent according to the types and the quantity of the microorganisms growing on the culture medium. Most of the common microbial cultures are cultured in Erlenmeyer flasks.
Chinese patent (publication No. CN 111004711A) discloses a microbial cultivation tank device, which comprises a stirring chamber, a stirring device and a cultivation tank main body, wherein the stirring device and the cultivation storage device constitute the cultivation tank main body, the bottom fixing of the cultivation tank main body is provided with a base, the stirring device is located above the cultivation storage device, the stirring device is internally provided with a stirring chamber and a transmission chamber, the top of the stirring chamber is provided with a feed inlet, the top of the transmission chamber is fixed with a low-speed motor through a bolt, the output end of the low-speed motor is provided with a rotary rod through a coupler, and the bottom fixing of the rotary rod is provided with oblique rotary rods which are uniformly distributed. The device utilizes the rotation difference between puddler and the rotatory cover to stir microorganism and biological raw materials in the culture tank in the microorganism culture tank, utilizes the different velocities to improve stirring homogeneity, improves the mixed degree, makes two kinds of biomaterial intensive mixing, but the device is comparatively complicated, and ordinary microorganism culture is mostly cultivateed in the erlenmeyer flask, therefore the device can't be used widely.
Disclosure of Invention
The present invention provides a microorganism culture apparatus which solves the problems set forth in the background art described above.
In order to achieve the purpose, the invention provides the following technical scheme:
a microorganism culture device comprises a fixed frame, wherein a first fixed plate is arranged in the middle of the lower surface of the fixed frame, the middle of the first fixed plate is rotatably connected with a first rotating shaft, a driving motor is arranged on the right side of the first fixed plate, an output shaft of the driving motor is fixedly connected with a first bevel gear, the first bevel gear is meshed with a second bevel gear, the second bevel gear is fixedly connected with the lower end of the first rotating shaft, the upper end of the first rotating shaft is fixedly connected with a driving gear, the side wall of the driving gear is meshed with a driven gear, the upper part of the driven gear is provided with a sliding sleeve, the upper part of the sliding sleeve is slidably connected with a sliding rod, the upper end of the sliding rod is provided with a spherical seat, the upper part of the spherical seat on the left side is fixedly connected with the left side of the lower surface of, wedge blocks are arranged on the left side and the right side of the fixing frame.
As a preferable technical scheme of the invention, one side of the wedge-shaped block close to the center of the device is an inclined surface, and the inclined surface of the wedge-shaped block is connected with the left end and the right end of the supporting plate in a sliding manner.
As a preferable technical scheme of the invention, the front side and the rear side of the lower surface of the fixed frame are provided with second fixed plates, the left side and the right side of each second fixed plate are rotatably connected with a second rotating shaft, and the upper end of each second rotating shaft is fixedly connected with a driven gear.
As a preferable technical solution of the present invention, the side edge of the driving gear is engaged with four driven gears.
As a preferred technical scheme of the invention, the middle part of the sliding sleeve is provided with a sliding cavity, the middle part of the sliding cavity is connected with a piston in a sliding way, and the middle part of the upper surface of the piston is fixedly connected with the lower end of a sliding rod.
As a preferable technical solution of the present invention, a tension spring is disposed between the bottom of the sliding chamber and the lower surface of the piston.
As a preferable technical scheme of the invention, the left side and the right side of the supporting plate are respectively provided with a placing table, and the axis of the placing table is collinear with the axis of the spherical seat.
As a preferred technical scheme of the invention, the side edge of the placing table is fixedly connected with the lower end of a fixing rod, the fixing rod is made of elastic material, and the middle part of the fixing rod is sunken towards the center of the placing table.
The invention has the following advantages:
the invention is suitable for a microorganism culture device, the four driven gears are driven to rotate by the driving gear arranged in the device, so that the supporting plate moves left and right, and in the process of moving the supporting plate left and right, the left and right ends of the supporting plate and the wedge block slide up and down, so that the left and right ends of the supporting plate tilt up and down, the movement of a conical flask is smoother, the mixing of culture solution in the conical flask is facilitated, the mixing of microorganisms is facilitated, the microorganisms are more uniformly dispersed in the culture solution, and the culture effect of the microorganisms is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of a microorganism culture apparatus.
FIG. 2 is a front view of a microorganism culture apparatus.
FIG. 3 is a top view of a microorganism culture apparatus.
Fig. 4 is a cross-sectional view a-a of fig. 3.
FIG. 5 is a schematic view showing the structure of a placing table in the microorganism culture apparatus.
FIG. 6 is a schematic view showing the structure of the slide bushing and the slide rod of the microorganism culture apparatus.
FIG. 7 is a bottom perspective view of a fixing frame of a microorganism culture apparatus.
In the figure: 1. a fixing frame; 2. a wedge block; 3. a support plate; 4. a placing table; 5. a second rotating shaft; 6. a first rotating shaft; 7. a second bevel gear; 8. a first bevel gear; 9. a drive motor; 10. a second fixing plate; 11. a first fixing plate; 12. a driven gear; 13. a driving gear; 14. a tension spring; 15. a sliding cavity; 16. a piston; 17. a sliding sleeve; 18. a slide bar; 19. a spherical seat; 20. fixing the rod; 21. a conical flask.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
Referring to fig. 1-7, a microorganism culture apparatus comprises a fixing frame 1, wherein a first fixing plate 11 is arranged in the middle of the lower surface of the fixing frame 1, the middle of the first fixing plate 11 is rotatably connected with a first rotating shaft 6, a driving motor 9 is arranged on the right side of the first fixing plate 11, an output shaft of the driving motor 9 is fixedly connected with a first bevel gear 8, the first bevel gear 8 is engaged with a second bevel gear 7, the second bevel gear 7 is fixedly connected with the lower end of the first rotating shaft 6, the upper end of the first rotating shaft 6 is fixedly connected with a driving gear 13, the side wall of the driving gear 13 is engaged with a driven gear 12, the side edges of the driving gear 13 are engaged with four driven gears 12, second fixing plates 10 are arranged on the front and rear sides of the lower surface of the fixing frame 1, the left and right sides of the second fixing plates 10.
The upper part of the driven gear 12 is provided with a sliding sleeve 17, the upper part of the sliding sleeve 17 is slidably connected with a sliding rod 18, the middle part of the sliding sleeve 17 is provided with a sliding cavity 15, the middle part of the sliding cavity 15 is slidably connected with a piston 16, a tension spring 14 is arranged between the bottom of the sliding cavity 15 and the lower surface of the piston 16, the middle part of the upper surface of the piston 16 is fixedly connected with the lower end of the sliding rod 18, the upper end of the sliding rod 18 is provided with a spherical seat 19, the left side of the upper part of the spherical seat 19 is fixedly connected with the left side of the lower surface of the supporting plate 3, the right side of the upper part of the spherical seat 19 is fixedly connected with the right side of the lower surface of the supporting plate 3, the upper surface of the supporting plate 3 is provided with a placing table 4, the left side and the right side of the supporting plate 3 are both provided with placing tables 4, the axis of the placing tables 4, the upper portion of placing platform 4 is equipped with erlenmeyer flask 21, the left and right sides of fixed frame 1 is equipped with wedge 2, and the one side that is close to the device center of wedge 2 is the inclined plane, the inclined plane sliding connection backup pad 3 of wedge 2 both ends about.
Example two
Referring to fig. 5, the other contents of the present embodiment are the same as those of the first embodiment, except that: the side of the placing table 4 is fixedly connected with the lower end of a fixing rod 20, the fixing rod 20 is made of elastic material, and the middle part of the fixing rod 20 is sunken towards the center direction of the placing table 4. In order to stably place the erlenmeyer flask 21 on the placing table 4, a plurality of fixing rods 20 are arranged on the placing table 4, the fixing rods 20 are made of elastic materials, meanwhile, the middle parts of the fixing rods 20 are sunken towards the middle of the placing table 4, therefore, when the erlenmeyer flask 21 needs to be clamped, only the bottom of the erlenmeyer flask 21 needs to be placed right above the placing table 4, the erlenmeyer flask 21 is pressed downwards, the bottom of the erlenmeyer flask 21 is contacted with the sunken parts in the middle parts of the fixing rods 20, the fixing rods 20 are pushed outwards in a driven mode, until finally the bottom of the erlenmeyer flask 21 is contacted with the placing table 4, the erlenmeyer flask 21 is stably fixed on the placing table 4, and when the erlenmeyer flask 21 is taken out, only the erlenmeyer flask.
In the implementation process of the invention, the device can be fixed inside relevant equipment, such as the inside of an incubator, or directly placed on a test bed, in the placing process, a cushion block is needed to be arranged at the bottom of the device to prevent a table top from contacting with a driving motor 9 of the device, after the device is fixed, a conical flask 21 can be taken out, a culture material of microorganisms is placed in the conical flask 21, after microorganism inoculation is completed, the conical flask 21 can be placed on a placing table 4, a fixing rod 20 at the side edge of the placing table 4 can stably fix the conical flask 21, at the moment, the driving motor 9 is started, an output shaft of the driving motor 9 drives a first rotating shaft 6 to rotate through a first bevel gear 8 and a second bevel gear 7 which are meshed and connected, the first rotating shaft 6 drives a driving gear 13 to rotate, the driving gear 13 drives four driven gears 12 to rotate, when the driven gears 12 rotate, the support plate 3 is driven to move left and right, the left end and the right end of the support plate 3 are in contact with the wedge-shaped block 2 in the moving process of the support plate 3, and the part of the wedge-shaped block 2 in contact with the support plate 3 is an inclined plane, so that the left end and the right end of the support plate 3 can be respectively tilted in the left-right moving process of the support plate 3, the support plate 3 is not in pure straight left-right moving, and can move back and forth in the left-right moving process, the final moving track of the conical flask 21 is complex, the culture solution in the conical flask 21 can be fully mixed, the moving mode of the track is dedicated to simulating the mode of manually shaking the conical flask 21, and the microbial; the invention is suitable for a microorganism culture device, a driving gear 13 is arranged in the device to drive four driven gears 12 to rotate, so that a support plate 3 moves left and right, and in the process of moving the support plate 3 left and right, the left and right ends of the support plate 3 and a wedge block 2 slide to enable the left and right ends of the support plate 3 to tilt up and down, so that the movement of a conical flask 21 is smoother, the mixing of a culture solution in the conical flask 21 is facilitated, the mixing of microorganisms is facilitated, the microorganisms are more uniformly dispersed in the culture solution, and the culture effect of the microorganisms is improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (8)

1. A microorganism culture device comprises a fixed frame (1), wherein a first fixed plate (11) is arranged in the middle of the lower surface of the fixed frame (1), the middle of the first fixed plate (11) is rotatably connected with a first rotating shaft (6), a driving motor (9) is arranged on the right side of the first fixed plate (11), an output shaft of the driving motor (9) is fixedly connected with a first bevel gear (8), the first bevel gear (8) is meshed with a second bevel gear (7), the second bevel gear (7) is fixedly connected with the lower end of the first rotating shaft (6), the microorganism culture device is characterized in that the upper end of the first rotating shaft (6) is fixedly connected with a driving gear (13), the side wall of the driving gear (13) is meshed with a driven gear (12), the upper part of the driven gear (12) is provided with a sliding sleeve (17), the upper part of the sliding sleeve (17) is slidably connected with a sliding, left side the lower surface left side of upper portion fixed connection backup pad (3) of spherical seat (19), right side the lower surface right side of upper portion fixed connection backup pad (3) of spherical seat (19), the upper surface of backup pad (3) is equipped with places platform (4), and the upper portion of placing platform (4) is equipped with erlenmeyer flask (21), the left and right sides of fixed frame (1) is equipped with wedge (2).
2. The microorganism culture device according to claim 1, wherein the wedge-shaped block (2) is provided with an inclined surface at one side close to the center of the device, and the inclined surface of the wedge-shaped block (2) is slidably connected with the left end and the right end of the support plate (3).
3. The microorganism culture device according to claim 1, wherein the front and back sides of the lower surface of the fixed frame (1) are provided with second fixed plates (10), the left and right sides of the second fixed plates (10) are rotatably connected with the second rotating shaft (5), and the upper end of the second rotating shaft (5) is fixedly connected with the driven gear (12).
4. A microorganism culture apparatus according to claim 1, wherein the driving gear (13) is engaged with four driven gears (12) at the side thereof.
5. The microorganism culture device according to claim 1, wherein the sliding sleeve (17) is provided with a sliding cavity (15) in the middle, the middle of the sliding cavity (15) is slidably connected with the piston (16), and the middle of the upper surface of the piston (16) is fixedly connected with the lower end of the sliding rod (18).
6. A microbial cultivation device as claimed in claim 5, characterised in that a tension spring (14) is provided between the bottom of the sliding chamber (15) and the lower surface of the piston (16).
7. The microorganism culture apparatus according to claim 1, wherein the left and right sides of the support plate (3) are provided with placing tables (4), and the axes of the placing tables (4) are collinear with the axes of the spherical seats (19).
8. The microorganism culture device according to claim 1, wherein the side of the placing table (4) is fixedly connected with the lower end of the fixing rod (20), the fixing rod (20) is made of elastic material, and the middle part of the fixing rod (20) is concave towards the center of the placing table (4).
CN202010760617.9A 2020-07-31 2020-07-31 Microbial cultivation device Withdrawn CN111849706A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010760617.9A CN111849706A (en) 2020-07-31 2020-07-31 Microbial cultivation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010760617.9A CN111849706A (en) 2020-07-31 2020-07-31 Microbial cultivation device

Publications (1)

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CN111849706A true CN111849706A (en) 2020-10-30

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CN202010760617.9A Withdrawn CN111849706A (en) 2020-07-31 2020-07-31 Microbial cultivation device

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114634855A (en) * 2022-03-08 2022-06-17 卢姚帆 Microbial beneficial bacterium culture apparatus
CN116616178A (en) * 2023-05-30 2023-08-22 湖南丰思农业发展有限公司 Rhododendron simsii subculture multiplication medium and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114634855A (en) * 2022-03-08 2022-06-17 卢姚帆 Microbial beneficial bacterium culture apparatus
CN116616178A (en) * 2023-05-30 2023-08-22 湖南丰思农业发展有限公司 Rhododendron simsii subculture multiplication medium and preparation method thereof

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