CN114365851A - Method for preparing wood frog egg glue peptide without enzymolysis process - Google Patents
Method for preparing wood frog egg glue peptide without enzymolysis process Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides a method for preparing wood frog egg glue peptide by an enzymolysis-free process, which comprises the steps of egg-laying frog selection, pretreatment before egg laying, egg laying preparation, fertilized egg treatment, egg glue peptide preparation, freeze drying for egg glue peptide preparation and the like. The invention combines the excellent variety breeding of the Chinese wood frog according to the embryonic developmental principle of the wood frog, adopts natural embracing to lay eggs in the breeding period of the commercial wood frog, utilizes the self-regulation and decomposition mechanism of the protease system of the wood frog embryo in the embryonic development process to decompose the egg collagen in the wood frog embryo into mainly polypeptide and oligopeptide, reduces the production cost, solves the problems of peculiar smell, high cost and complicated operation procedures of artificial enzymolysis products, adopts the method of 'breeding the frog and taking the eggs', ensures that adult female frogs can be continuously utilized, produces original ecological products, changes the traditional method of 'killing the frog and taking oil', protects ecological balance, increases both production and income, and promotes the sustainable development of standing.
Description
Technical Field
The invention relates to a preparation method of wood frog (Chinese forest frog) egg glue peptide, in particular to a method for preparing wood frog egg glue peptide without an enzymolysis process.
Background
The wood frog, which is commonly referred to as "Rana dybowski" is a tailless amphibian of Ranidae and Rana genus. The main products of northeast mountain areas of China, including Changbai mountain, major part of lesser Khingan mountain and abdomen of Zhangguangdi mountain, also called forest frog and forest frog, are rare species integrating medicinal, dietetic and cosmetic functions, and the products have the functions of delaying senility and enhancing immunity, so the forest frog breeding industry has become an important industry for the development of northeast mountain areas of China. In the process of industrial development, hundreds of millions of wood frogs are killed to obtain wood frog oil (oviducts) every year in order to obtain wood frog oil products, but byproducts such as ova rich in nutrition are discarded, so that a great deal of resource waste is caused, and meanwhile, huge threats are brought to ecological environment and germplasm resource protection.
In order to fully develop and utilize wood frog resources, people begin to carry out all-round research work on wood frogs. The intensive research on the action mechanism of peptides in living bodies finds that peptide substances are small molecular substances polymerized from a plurality of amino acid molecules, protein is decomposed into peptide substances with low molecular weight, the peptide substances are easier to digest and absorb by human bodies than the protein, and the peptide substances also contain polypeptide with special functions, so that a plurality of production methods related to the preparation of peptides such as wood frog oil, meat, skin and the like are researched, for example, a method for preparing the wood frog oil peptide by using the wood frog oil is used, and fresh wood frog oil or dried wood frog oil is taken and added with protease to prepare the wood frog oil peptide through enzymolysis.
The preparation methods of the oviductus ranae peptide disclosed by the existing literature documents are more, the relevant data of the oviductus ranae peptide is not reported, and the existing preparation process of the peptide has many problems: the peptide product is obtained by adopting a method of carrying out enzymolysis on protein by using protease such as trypsin, papain and the like, but the product can generate peculiar smell after the protease is used for carrying out enzymolysis on the protein, so that the taste is influenced; meanwhile, as the hydrolysis degree is deepened, the content of free estradiol with active ingredients in the rana japonica oil peptide is gradually increased, so that the content of estrogen in the rana japonica oil peptide is increased, and the rana japonica oil peptide is not beneficial to most people to eat, so that the eating population and popularization range of the rana japonica oil are limited; enzyme deactivation treatment is needed after enzymolysis, so the preparation process is complicated in procedure and high in production cost; the wood frog needs to be killed to obtain the wood frog oil raw material, so that the sustainability is poor, and the industrial development and the resource protection are not facilitated. In view of the above, there is a need to provide a low-cost preparation method of a wood frog peptide, which can continuously utilize wood frog resources, reduce resource waste, and does not need to add protease, and is suitable for all ages and both sexes, and to promote the healthy development of wood frog resources.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for preparing wood frog egg glue peptide by an enzymolysis-free process, which comprises the following steps:
(1) spawning frog selection:
in the eastern mountain area of China, 4-5 months every year, after the wood frogs hibernate, uniformly selecting good varieties according to a seed reserving standard, reserving the good varieties for reproduction, and screening unqualified male and female wood frogs for spawning collagen peptide;
(2) pretreatment before spawning:
separating and washing the screened female and male wood frogs for producing the spawning glue peptide, and respectively entering adjacent pre-heating ponds for pre-heating, wherein the water temperature in the pre-heating ponds is 18-22 ℃, the natural illumination is carried out, and the density of the frogs is less than 500 per square meter;
(3) spawning preparation:
after pretreatment is carried out for 2-3 days, affected by frogming and temperature, when female frogs enter a spawning period, respectively fishing out and washing the female frogs and the male frogs from a prewarming pool, mixing the female frogs and the male frogs according to the proportion of 1: 1.2-1.5 of the number of the female frogs and the male frogs, putting the male frogs and the female frogs into a spawning pool, wherein the water temperature of the spawning pool is 18-22 ℃, natural illumination is carried out, and the density of the frogs is less than 40 per square meter;
(4) fertilized egg pretreatment:
taking out the eggs by using a string bag after the eggs are laid for 15-30 minutes (fully fertilized), cleaning the surfaces of the egg masses by using purified water or mineral water, putting the eggs into an egg development room with the ambient temperature of 22-24 ℃ and the relative humidity of 70-85 percent, placing the eggs on a development plate, placing the eggs in a single layer, exposing the eggs in the air, spraying water to the egg masses at proper time, keeping the surfaces of the egg masses moist, and enabling the fertilized eggs to normally develop by using the light supplement illumination of 3000 plus 4000 luxes;
(5) preparing the egg glue peptide:
and when the fertilized eggs are developed for 12-18 hours, terminating the development, grinding the mixture of the fertilized eggs and the egg glue at a high speed by a colloid mill to the fineness of more than 100 meshes to obtain viscous porridge-like fluid, adding 20-50% of purified water for dilution, and then feeding the mixture into a homogenizer for homogenizing for 10-15 minutes. After homogenizing, the mixture enters a centrifuge, the mixture is centrifuged for 5-10 minutes at the rotation speed of 4000-;
(6) drying to prepare the lecithin peptide:
and (3) drying the supernatant in a freeze dryer, wherein the drying parameters are as follows: the prefreezing temperature is-30 ℃ to-33 ℃, the material thickness is less than 1.5cm, the vacuum degree is 60Pa to 120Pa, the drying time is 25 to 35 hours, and the snowflake solid lecithin peptide product with the water content of less than 15 percent is obtained after drying, and can be directly applied to solid drinks, tablets, granules and food additives.
The seed reserving standard is a Chinese forest frog Changbai mountain subspecies with a back with a herringbone black line mark; the frog is more than three years old; male body weight > 22g, female body weight > 35 g; there was no visible disease.
Preferably, the depth of the preheating tank is 0.7-0.8 m, the width of the preheating tank is 1.5-2.5 m, the length of the preheating tank is not less than 2m, and the tank wall is vertical; and (4) putting the river water of the wood frog producing area into the preheating pool, wherein the water depth is 0.3-0.4 m.
Preferably, the material of the spawning pond is 304 stainless steel or food-grade plastic, the height of the spawning pond is 0.4-0.5 m, the width of the spawning pond is 0.5-1 m, the length of the spawning pond is not less than 1m, and the walls of the spawning pond are vertical; edible purified water is put into the spawning pond, and the water depth is 0.1-0.15 m.
Preferably, the female frogs are timely fished out after laying eggs in the step (3), placed into a breeding farm for the postpartum frogs within 5 hours, and bred conventionally after reproduction dormancy, and multiple layers of overlapping and high temperature are avoided in the transportation process of the postpartum frogs; the male frogs are fished out to be used as commercial frogs.
The working principle of the invention is as follows:
according to the principles of wood frog embryo development, the invention discovers that after the wood frog is held by arms to lay eggs, oosperm which grows for less than 12 hours still exists in a form of taking protein as a main form, and belongs to the category of modified wood frog oil; when the fertilized eggs develop for more than 12 hours, the effective components of the fertilized eggs (including the oosperm membrane) are activated mostly, and the activated gene expression changes the original attributes of the raw materials forming the framework of the living body so as to adapt to the requirements of a new living body, for example, estrogen finishes the historical mission, is attenuated and is exhausted, and releases various life active substances, such as growth hormone, juvenile hormone and the like; meanwhile, the protein is gradually decomposed into polypeptide, oligopeptide and amino acid under the action of an enzyme system, and a material basis is laid for the construction and growth of new life bodies. With the development of the embryo, the rate of decomposing the protein into polypeptide and oligopeptide is closely related to the development time, temperature and illumination, the content percentage of the polypeptide and the oligopeptide is in direct proportion to the development time of the embryo, the estrogen content is in inverse proportion to the development time of the embryo, and the content of hormones such as estradiol, estriol, estrone, testosterone, diethylstilbestrol and the like in the wood frog embryo after the fertilized egg develops for 12-18 hours is 0. Therefore, the key of the technology is to control the processing for preparing the egg collagen peptide before the metabolic products do not appear after the embryo is developed to the blastocyst stage and when the peptide content is rich, and stop the development of the wood frog embryo.
The invention has the beneficial effects that:
the method combines the breeding of the excellent variety of the rana chensinensis, fully develops the utilization value of the commercial rana chensinensis while protecting the germplasm resources of the rana chensinensis, adopts natural embracing of the commercial rana chensinensis in the breeding period to lay eggs, utilizes the self-regulation and decomposition mechanism of the protease system of the embryo of the rana chensinensis in the embryo development process to decompose the egg collagen in the embryo of the rana chensinensis into polypeptide and oligopeptide, avoids the peculiar smell generated by the protease added manually on the egg collagen peptide and influences the taste, reduces the production cost, solves the problems of peculiar smell, high cost and complicated operation procedures of the artificial enzymolysis product, adopts the method of 'breeding rana chensinensis and taking eggs', ensures the sustainable utilization of the adult female rana chensinensis, produces original ecological products, changes the traditional method of 'killing and taking oil', protects the ecological balance, increases the yield and income, and promotes the sustainable development of standing.
Detailed Description
The first embodiment,
The embodiment provides a method for preparing wood frog egg glue peptide by an enzymolysis-free process, which comprises the following steps:
(1) spawning frog selection:
in the eastern mountain area of China, 4-5 months every year, after the wood frogs hibernate, uniformly selecting excellent varieties according to a seed reserving standard, wherein the seed reserving standard is a Chinese wood frog Changbai mountain subspecies with a character-shaped black line mark at the back; the frog is more than three years old; male body weight > 22g, female body weight > 35 g; there was no visible disease. The fine variety is reserved for propagation, and unqualified male and female wood frogs are screened out to be used for generating egg glue peptide;
(2) pretreatment before spawning:
separating and washing the screened female and male wood frogs for producing spawning glue peptide, and respectively entering adjacent pre-heating ponds for pre-heating, wherein the depth of each pre-heating pond is 0.7-0.8 m, the width of each pre-heating pond is 1.5-2.5 m, the length of each pre-heating pond is not less than 2m, and the walls of the pre-heating ponds are vertical; putting river water of a wood frog producing area into the preheating pool, wherein the water depth is 0.3-0.4 m; the water temperature in the pre-heating pool is 18-22 ℃, natural illumination is carried out, and the density of frogs is less than 500 per square meter;
(3) spawning preparation:
after pretreatment is carried out for 2-3 days, affected by frogming and temperature, when female frogs enter a spawning period, respectively fishing out and washing the female frogs and the male frogs from a preheating pond, mixing the female frogs and the male frogs according to the proportion of 1: 1.2-1.5 of the number of the female frogs and the male frogs, putting the male frogs and the female frogs into a spawning pond, wherein the spawning pond is made of 304 stainless steel or food-grade plastics, the height of the spawning pond is 0.4-0.5 m, the width of the spawning pond is 0.5-1 m, the length of the spawning pond is not less than 1m, and the walls of the spawning pond are vertical; edible purified water is put into the spawning pond, and the water depth is 0.1-0.15 m; the water temperature of the spawning pond is 18-22 ℃, natural illumination is carried out, and the density of the frogs is less than 40 per square meter;
timely fishing out the female frogs after laying the eggs, putting the female frogs into a postpartum frog breeding farm within 5 hours after delivery, carrying out conventional breeding after reproduction dormancy, and avoiding multilayer overlapping and high temperature in the transportation process of the female frogs; the male frogs are fished out to be used as commercial frogs.
(4) Fertilized egg pretreatment:
taking out the eggs by using a string bag after the eggs are laid for 15-30 minutes (fully fertilized), cleaning the surfaces of the egg masses by using purified water or mineral water, putting the eggs into an egg development room with the ambient temperature of 22-24 ℃ and the relative humidity of 70-85 percent, placing the eggs on a development plate, placing the eggs in a single layer, exposing the eggs in the air, spraying water to the egg masses at proper time, keeping the surfaces of the egg masses moist, and enabling the fertilized eggs to normally develop by using the light supplement illumination of 3000 plus 4000 luxes;
(5) preparing the egg glue peptide:
when fertilized eggs develop for 12 hours, terminating development, grinding the mixture of the fertilized eggs and egg glue at a high speed by a colloid mill to the fineness of more than 100 meshes to obtain viscous porridge-like fluid, adding 20-50% of purified water, diluting, then feeding the mixture into a homogenizer, homogenizing for 10 minutes, after the homogenization, feeding the mixture into a centrifuge, centrifuging at the rotation speed of 4000-; (6) drying to prepare the lecithin peptide:
and (3) drying the supernatant in a freeze dryer, wherein the drying parameters are as follows: the prefreezing temperature is-30 ℃ to-33 ℃, the material thickness is less than 1.5cm, the vacuum degree is 60Pa to 120Pa, the drying time is 25 to 35 hours, and the snowflake-shaped egg glue peptide product with the water content of less than 15 percent is obtained after drying, and can be directly applied to solid drinks, tablets, granules and food additives.
Example II,
The embodiment provides a method for preparing wood frog egg glue peptide by an enzymolysis-free process, which comprises the following steps:
(1) spawning frog selection:
in the eastern mountain area of China, 4-5 months every year, after the wood frogs hibernate, uniformly selecting excellent varieties according to a seed reserving standard, wherein the seed reserving standard is a Chinese wood frog Changbai mountain subspecies with a character-shaped black line mark at the back; the frog is more than three years old; male body weight > 22g, female body weight > 35 g; the good varieties without visible diseases are reserved for propagation, and unqualified male and female wood frogs are screened out to be used for generating egg glue peptides;
(2) pretreatment before spawning:
separating and washing the screened female and male wood frogs for producing spawning glue peptide, and respectively entering adjacent pre-heating ponds for pre-heating, wherein the depth of each pre-heating pond is 0.7-0.8 m, the width of each pre-heating pond is 1.5-2.5 m, the length of each pre-heating pond is not less than 2m, and the walls of the pre-heating ponds are vertical; putting river water of a wood frog producing area into the preheating pool, wherein the water depth is 0.3-0.4 m; the water temperature in the pre-heating pool is 18-22 ℃, natural illumination is carried out, and the density of frogs is less than 500 per square meter;
(3) spawning preparation:
after pretreatment is carried out for 2-3 days, affected by frogming and temperature, when female frogs enter a spawning period, respectively fishing out and washing the female frogs and the male frogs from a preheating pond, mixing the female frogs and the male frogs according to the proportion of 1: 1.2-1.5 of the number of the female frogs and the male frogs, putting the male frogs and the female frogs into a spawning pond, wherein the spawning pond is made of 304 stainless steel or food-grade plastics, the height of the spawning pond is 0.4-0.5 m, the width of the spawning pond is 0.5-1 m, the length of the spawning pond is not less than 1m, and the walls of the spawning pond are vertical; edible purified water is put into the spawning pond, and the water depth is 0.1-0.15 m; the water temperature of the spawning pond is 18-22 ℃, natural illumination is carried out, and the density of the frogs is less than 40 per square meter;
timely fishing out the female frogs after laying the eggs, putting the female frogs into a postpartum frog breeding farm within 5 hours after delivery, carrying out conventional breeding after reproduction dormancy, and avoiding multilayer overlapping and high temperature in the transportation process of the female frogs; the male frogs are fished out to be used as commercial frogs.
(4) Fertilized egg pretreatment:
taking out the eggs by using a string bag after the eggs are laid for 15-30 minutes (fully fertilized), cleaning the surfaces of the egg masses by using purified water or mineral water, putting the eggs into an egg development room with the ambient temperature of 22-24 ℃ and the relative humidity of 70-85 percent, placing the eggs on a development plate, placing the eggs in a single layer, exposing the eggs in the air, spraying water to the egg masses at proper time, keeping the surfaces of the egg masses moist, and enabling the fertilized eggs to normally develop by using the light supplement illumination of 3000 plus 4000 luxes;
(5) preparing the egg glue peptide:
stopping development when fertilized eggs develop for 18 hours, grinding the mixture of the fertilized eggs and the egg glue at a high speed by a colloid mill to the fineness of more than 100 meshes to obtain viscous porridge-like fluid, adding 20-50% of purified water, diluting, then feeding the mixture into a homogenizer, homogenizing for 10 minutes, feeding the homogenized mixture into a centrifuge, centrifuging at the rotation speed of 4000-;
(6) drying to prepare the lecithin peptide:
and (3) drying the supernatant in a freeze dryer, wherein the drying parameters are as follows: the prefreezing temperature is-30 ℃ to-33 ℃, the material thickness is less than 1.5cm, the vacuum degree is 60Pa to 120Pa, the drying time is 25 to 35 hours, and the product of the egg glue peptide with the water content of less than 15 percent is obtained after drying, and can be directly applied to solid drinks, tablets, granules and food additives.
Through detection, the contents of the glue peptide and the estrogen of the fertilized eggs (embryos) of the wood frogs are as follows:
Claims (4)
1. a method for preparing wood frog egg glue peptide without enzymolysis procedure is characterized in that: the method comprises the following steps:
(1) spawning frog selection:
4-5 months per year, after the wood frogs hibernate, uniformly selecting good varieties according to a seed reserving standard, reserving the good varieties for reproduction, and screening unqualified male and female wood frogs for producing egg glue peptides;
(2) pretreatment before spawning:
separating and washing the screened female and male wood frogs for producing the spawning glue peptide, and respectively entering adjacent pre-heating ponds for pre-heating, wherein the water temperature in the pre-heating ponds is 18-22 ℃, the natural illumination is carried out, and the density of the frogs is less than 500 per square meter;
(3) spawning preparation:
after pretreatment is carried out for 2-3 days, affected by frogming and temperature, when female frogs enter a spawning period, respectively fishing out and washing the female frogs and the male frogs from a prewarming pool, mixing the female frogs and the male frogs according to the proportion of 1: 1.2-1.5 of the number of the female frogs and the male frogs, putting the male frogs and the female frogs into a spawning pool, wherein the water temperature of the spawning pool is 18-22 ℃, natural illumination is carried out, and the density is less than 40 per square meter;
(4) fertilized egg pretreatment:
taking out the eggs after the eggs are laid for 15-30 minutes, cleaning the surfaces of the eggs by using purified water or mineral water, entering an egg development room with the ambient temperature of 22-24 ℃ and the relative humidity of 70-85%, placing the eggs on a development board in a single-layer mode, exposing the eggs in the air, spraying water to the eggs at proper time, keeping the surfaces of the eggs moist, and enabling the fertilized eggs to normally develop under the condition of 3000-plus-one-4000 lux light;
(5) preparing the egg glue peptide:
stopping development when fertilized eggs develop for 12-18 hours, grinding the mixture of the fertilized eggs and the egg glue at a high speed by a colloid mill to the fineness of more than 100 meshes to obtain viscous porridge-like fluid, adding 20-50% of purified water for dilution, then feeding the diluted mixture into a homogenizer for homogenizing for 10-15 minutes, feeding the homogenized mixture into a centrifuge at the rotation speed of 4000-;
(6) drying to prepare the lecithin peptide:
and (3) drying the supernatant in a freeze dryer, wherein the drying parameters are as follows: the prefreezing temperature is-30 ℃ to-33 ℃, the material thickness is less than 1.5cm, the vacuum degree is 60Pa to 120Pa, the drying time is 25 to 35 hours, and the ovopeptine snowflake solid product with the water content of less than 15 percent is obtained after drying.
2. The method for preparing the wood frog egg glue peptide by an enzymolysis-free process according to claim 1, which comprises the following steps: the depth of the preheating tank is 0.7-0.8 m, the width of the preheating tank is 1.5-2.5 m, the length of the preheating tank is not less than 2m, and the tank wall is vertical; and (4) putting the river water of the wood frog producing area into the preheating pool, wherein the water depth is 0.3-0.4 m.
3. The method for preparing the wood frog egg glue peptide by an enzymolysis-free process according to claim 1, which comprises the following steps: the spawning pond is made of 304 stainless steel or food-grade plastic, the height of the spawning pond is 0.4-0.5 m, the width of the spawning pond is 0.5-1 m, the length of the spawning pond is not less than 1m, and the walls of the spawning pond are vertical; edible purified water is put into the spawning pond, and the water depth is 0.1-0.15 m.
4. The method for preparing the wood frog egg glue peptide by an enzymolysis-free process according to claim 1, which comprises the following steps: timely fishing out the female frogs after laying the eggs, putting the female frogs into a postpartum frog breeding farm within 5 hours after delivery, carrying out conventional breeding after reproduction dormancy, and avoiding multilayer overlapping and high temperature in the transportation process of the postpartum frogs; the male frogs are fished out to be used as commercial frogs.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113698981A (en) * | 2021-08-31 | 2021-11-26 | 吉林省蛙王生物工程有限公司 | Production method of modified wood frog oil |
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| WO1982004443A1 (en) * | 1981-06-12 | 1982-12-23 | Ohio Univ | Genetic transformation of zygotes |
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| CN113698981A (en) * | 2021-08-31 | 2021-11-26 | 吉林省蛙王生物工程有限公司 | Production method of modified wood frog oil |
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| WO1982004443A1 (en) * | 1981-06-12 | 1982-12-23 | Ohio Univ | Genetic transformation of zygotes |
| CN1820644A (en) * | 2006-03-24 | 2006-08-23 | 白秀娟 | Process for preparing frog embryo health food |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113698981A (en) * | 2021-08-31 | 2021-11-26 | 吉林省蛙王生物工程有限公司 | Production method of modified wood frog oil |
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