CN102960559B - Method for Feed for artificially raising Dastarcus helophoroides in large scale - Google Patents
Method for Feed for artificially raising Dastarcus helophoroides in large scale Download PDFInfo
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- CN102960559B CN102960559B CN 201210474799 CN201210474799A CN102960559B CN 102960559 B CN102960559 B CN 102960559B CN 201210474799 CN201210474799 CN 201210474799 CN 201210474799 A CN201210474799 A CN 201210474799A CN 102960559 B CN102960559 B CN 102960559B
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Abstract
The invention provides a feed for artificially raising Dastarcus helophoroides in a large scale and a matched breeding technology of the feed. The invention discloses the feed for artificially raising the Dastarcus helophoroides in the large scale. The feed is prepared by taking yellow mealworm larva dry powder, barley pest larva dry powder and sterile water as raw materials and matching with various amino acids, linoleic acid and glucose; and the preparation manner is simple, raw materials are cheap and are easily available, and the feed has high economic value. Meanwhile, the invention further discloses the matched breeding technology of the feed for artificially raising the Dastarcus helophoroides in the large scale; the feed is matched with a preservation box and de-greased cotton to carry out hatching operation of the Dastarcus helophoroides; an artificial weather box is matched to obtain a shorter breeding period when being compared with the prior art; and meanwhile, high maturelarva survival rate can be obtained.
Description
Technical field
The present invention relates to artificial feed preparation and the extensive raising technology of coleopteron larva, relate in particular to a kind of artificial feed prescription and raising technology of extensive raising dastarcus helophoroides.
Background technology
In China's agricultural and forestry production, longicorn is the very serious insect of class harm, comprising: the frequent specieses such as Monochamus alternatus, anoplophora glabripennis, mountain longicorn, batocera horsfieldi, mulberry longicorn.Their adult supplements the nutrients mainly with gnawing trees spray skin, causes the host weak; Larva is bored the moth trunk in a large number, causes that trees roll in flakes, death.In addition, the adult of some kind or the communication media of pine forest destructive disease are exactly the main communication media of pine nematode such as Monochamus alternatus, and in a single day pine forest infects pine nematode, will cause the destructive Large Scale Death of pine forest.Because longicorn class pest life is very hidden, all one's life, most of time was lived in trunk, therefore in actual production extremely difficulty adopt an effective measure and prevent and treat.
The natural enemy insects such as release dastarcus helophoroides can solve the problem of preventing and treating of longicorn preferably.Show according to documents and materials: the longicorn beetle dastarcus helophoroides can reach more than 80% to the control efficiency of anoplophora glabripennis; The piebald dastarcus helophoroides is about 90% to the parasitic rate of luring Monochamus alternatus in the wood; The mallambyx raddei dastarcus helophoroides to the control efficiency of mountain longicorn generally more than 85%.This shows, the harm effect that discharges dastarcus helophoroides control agricultural longicorn is remarkable.And add the generation of natural enemy environmentally safe and energy Sustainable Control insect, therefore breed on a large scale dastarcus helophoroides control longicorn class pest China's agricultural and forestry production is had safely very important realistic meaning.
The artificial feed of research natural enemy insect are the bases of extensive raising technology.Artificial feed need not raised the Hosts live bodies with a large amount of human and material resources, are not subjected to seasonal restriction, do not need every day and increase or change the host.Can in less space, produce a large amount of products, can reduce because of the impaired courses of infection that causes of Hosts live body and corruption.And use artificial feed to raise the result who draws, data information is detailed, and purpose is strong, can provide the food of joining with strong points according to the insect with different qualities, can be used as and analyzes the foundation that insect grows the desired nutritional composition.
At present, the major area that dastarcus helophoroides is bred by China concentrates on Beijing, Hunan, Chongqing, zhejiang and other places, year breed dastarcus helophoroides imago quantity to be no more than 3,000,000, and nearly 6,000,000 yuan of the expenses such as artificial, the energy that drops into, material.Be that every dastarcus helophoroides imago production cost reaches about 2 yuan.Production cost is too high, be since now domestic all be to adopt traditional raising technology, namely utilizing the Hosts artificial infection to breed.The operation of this method generally is: Hosts is cultivated---artificial infection (selecting one by one with writing brush)---and is packed cultivation.Under this pattern, in Hosts cultivation, artificial infection and packaging process, need to consume a large amount of energy and artificial, operating efficiency is extremely low.Therefore, expensive must causing is difficult to be accepted at the conceited Under the market economy condition that loses of winning of current self-employed tree cultivator.Studying a kind of operation, simple, with low cost to be applicable to artificial feed and raising technology that all dastarcus helophoroides breed on a large scale necessary.
Summary of the invention
The object of the present invention is to provide a kind of artificial extensive feed and supporting raising technology thereof of raising dastarcus helophoroides, under this technical conditions, operational sequence is simple, cost is low, be suitable for raising on a large scale dastarcus helophoroides.Simultaneously, artificial feed of the present invention are not only applicable to the raising and research in laboratory, more are applicable to batch production large-scale production dastarcus helophoroides.
The present invention at first provides a kind of artificial extensive feed of raising dastarcus helophoroides, and this feed formula comprises following several component (in weight portion):
Yellow meal worm larva dry powder: 20% ~ 30%;
Barley worm larva dry powder: 10%～15%;
Sterile water: 60%～70%;
Proline: 0.01%～0.05%;
Hydroxyamino: 0.01%～0.05%;
Serine: 0.01%～0.05%;
Cystine: 0.05%～0.1%;
Glucose: 2%～5%;
Linoleic acid: 2%～5%.
Above-mentioned 8 kinds of components are that artificial feed of the present invention prescription is necessary, the technical staff can add according to actual needs in sorbic acid, Sodium Benzoate, the natamycin one or more carry out preservative treatment;
The artificial feed of dastarcus helophoroides of the present invention make as follows:
A), Yellow meal worm larva dry powder and barley worm larva dry powder blend is even;
B), the mixture of step a adds sterile water under the condition that stirs, this solution left standstill 12h for preparing is for subsequent use;
C), in the solution of step b, add amino acid (comprising above-mentioned proline, hydroxyamino, serine, cystine) and linoleic acid, mix the artificial feed that can obtain dastarcus helophoroides;
The extensive raising technology of dastarcus helophoroides of the present invention is based on that artificial extensive feed of raising dastarcus helophoroides realizes, it is as follows that it comprises step:
1., above-mentioned artificial feed are poured in the crisper that is covered with one deck absorbent cotton, make this solution of the even absorption of absorbent cotton;
2., the dastarcus helophoroides ovum card that will soon hatch, evenly be placed in the crisper that 1. step is made, cover the lid of crisper after, make shading treatment with black cloth parcel crisper;
3., the crisper of will be 2. having put into the dastarcus helophoroides ovum in the step is placed on climatic cabinate and cultivated 7-10 days, can obtain the mature larva of a large amount of dastarcus helophoroides;
4., in 3. step in the crisper the certain thickness absorbent cotton of cultured dastarcus helophoroides mature larva upper berth one deck dastarcus helophoroides is cocoond pupate;
5., will be 4. the crisper of step be placed on to cultivate in the climatic cabinate and can obtain a large amount of dastarcus helophoroides imagos in 40-60 days.
According to the solution of the present invention, described step is the long 20 ~ 25cm of middle crisper 1., wide 8～10cm, and high 5～8cm, spreading the absorbent cotton thickness that enters is 0.3～0.5cm, the artificial feed that prepare in (1) step of evenly pouring in each crisper are 60～80g.
According to the solution of the present invention, the dastarcus helophoroides ovum quantity that is about to hatching that described step is put in 2. is 200～400, has one of ovum to face up, and is placed on the absorbent cotton.
According to the solution of the present invention, described step 3. middle climatic cabinate temperature setting is set to 24～28 ℃, and humidity is set to 65%～80%, and illuminance is 0, and incubation time is 7-10 days.
According to the solution of the present invention, the described step 4. absorbent cotton thickness that enters of middle berth is 0.5～1cm, all the dastarcus helophoroides mature larva is covered.
According to the solution of the present invention, described step 5. in the climatic cabinate temperature setting be set to 24～28 ℃, humidity is set to 75%～80%, illuminance is 0, incubation time is can obtain 200～400 dastarcus helophoroides imagos in 40～60 days.
Advantage of the present invention and beneficial effect are:
(1) dastarcus helophoroides artificial feed comprehensive nutrition of the present invention, and raw material sources are easy, cheap.Prepare 1 ton of its cost of material of dastarcus helophoroides artificial feed and be roughly equal to 16000 yuan of RMB.And artificial feed conservative estimation per ton can be bred 3,000,000 of dastarcus helophoroides, the equal highly significant of economic benefit and social benefit.
(2) dastarcus helophoroides artificial feed compound method of the present invention is easy, breeds operation simple.It is 90% artificial that relatively existing operation can be saved, and saved and bred Hosts, selected fresh Substitute Host Pupae, manually the process such as packed with writing brush inoculation, artificial tubulature.
(3) use artificial feed of the present invention and raising technology efficient very high.1 those skilled in the art can prevent and treat approximately 60,000 mu of longicorn hazard areas in the situation that equipment and material conditions being possessed approximately can be bred 3,000,000 of dastarcus helophoroides imagos in 1 year, and this can solve the now low problem of dastarcus helophoroides production efficiency greatly.
(4) the present invention not only is applicable to the dastarcus helophoroides artificial breeding, can be used for breeding the multiple coleoptera natural enemy insects such as ladybug, ground beetle, tiger beetle on the basis of adding other auxiliary material such as pork liver powder, agar.
(5) feed of the present invention is stable in order to the dastarcus helophoroides sex character of continuous raising, does not find more than 15 generations that degradation phenomena appears in dastarcus helophoroides.
Embodiment
The below gives an actual example and describes the present invention.
Embodiment 1:
At first, with Yellow meal worm larva dry powder 2500g, 1250g is even in stirrer for mixing for barley worm larva dry powder, adds the 7000g sterile water in agitator, and this solution left standstill 12h for preparing is for subsequent use; In mixed liquor, add while stirring proline 2.5g behind the 12h, hydroxyamino 2.5g, serine 2.5g, cystine 50g, glucose 350g, linoleic acid 350g mixes all components.
Then, prepare 100 long 25cm, wide 10cm, the crisper of high 8cm at the absorbent cotton of each crisper middle berth one deck 0.3cm, is poured the artificial feed that 80g prepares in each crisper, make that absorbent cotton evenly adsorbs the artificial feed of preparation in the crisper; With 400 dastarcus helophoroides ovum cards that are about to hatching, evenly be placed in the crisper of making again, cover the lid of crisper after, make shading treatment with black cloth parcel crisper; Then crisper is placed on temperature and is 25 ℃, humidity and be 65%, illuminance is to cultivate 10 days in 0 the climatic cabinate, obtains at last 32547 of the mature larvas of dastarcus helophoroides.Impelling dastarcus helophoroides to cocoon at the thick absorbent cotton of dastarcus helophoroides mature larva upper berth one deck 0.5cm subsequently pupates, again this crisper is put into temperature and is 25 ℃, humidity and be 75%, illuminance is that 0 climatic cabinate is cultivated 31786 of the healthy adults of last bred dastarcus helophoroides.
Raise dastarcus helophoroides with this artificial feed, larva is on average gone through 8.5 days phases, and pupa is on average gone through 45.9 days phases, and it is 81.37% that larvae development becomes the success rate of mature larva, mature larva grow to the success rate of adult be 97.67%.
Embodiment 2:
At first, with Yellow meal worm larva dry powder 6000g, 3000g is even in stirrer for mixing for barley worm larva dry powder, adds the 14000g sterile water in agitator, and this solution left standstill 12h for preparing is for subsequent use; In mixed liquor, add while stirring proline 8g behind the 12h, hydroxyamino 8g, serine 8g, cystine 120g, glucose 800g, linoleic acid 800g receives his mould malicious 10g, and all components is mixed.
Then, prepare 200 long 20cm, wide 8cm, the crisper of high 5cm at the absorbent cotton of each crisper middle berth one deck 0.3cm, is poured the artificial feed that 80g prepares in each crisper, make that absorbent cotton evenly adsorbs the artificial feed of preparation in the crisper; With 380 dastarcus helophoroides ovum cards that are about to hatching, evenly be placed in the crisper of making again, cover the lid of crisper after, make shading treatment with black cloth parcel crisper; Then crisper is placed on temperature and is 25 ℃, humidity and be 70%, illuminance is to cultivate 10 days in 0 the climatic cabinate, obtains at last 62852 of the mature larvas of dastarcus helophoroides.Impelling dastarcus helophoroides to cocoon at the thick absorbent cotton of dastarcus helophoroides mature larva upper berth one deck 0.5cm subsequently pupates, again this crisper is put into temperature and is 26 ℃, humidity and be 80%, illuminance is that 0 climatic cabinate is cultivated 57912 of the healthy adults of last bred dastarcus helophoroides.
Raise dastarcus helophoroides with this artificial feed, larva is on average gone through 8.4 days phases, and pupa is on average gone through 44.8 days phases, and it is 82.70% that larvae development becomes the success rate of mature larva, mature larva grow to the success rate of adult be 92.14%.Feed is without corrupt, long miscellaneous bacteria phenomenon.
Above demonstration and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (7)
1. an artificial extensive method of raising dastarcus helophoroides is characterized in that, feeding process comprises following operating procedure:
1., previously prepared artificial extensive feed of raising dastarcus helophoroides is poured in the crisper that is covered with one deck absorbent cotton, make this solution of the even absorption of absorbent cotton;
2., the dastarcus helophoroides ovum card that will soon hatch, evenly be placed in the crisper that 1. step is made, cover the lid of crisper after, make shading treatment with black cloth parcel crisper;
3., the crisper of will be 2. having put into the dastarcus helophoroides ovum in the step is placed on climatic cabinate and cultivated 7-10 days, can obtain the mature larva of a large amount of dastarcus helophoroides;
4., in 3. step in the crisper cultured dastarcus helophoroides mature larva upper berth one deck absorbent cotton dastarcus helophoroides is cocoond pupate;
5., will be 4. the crisper of step be placed on to cultivate in the climatic cabinate and can obtain a large amount of dastarcus helophoroides imagos in 40-60 days;
And in above-mentioned steps, described artificial extensive feed of raising dastarcus helophoroides is made by the raw material of following each percentage by weight component:
Yellow meal worm larva dry powder: 20% ~ 30%; Barley worm larva dry powder: 10%～15%;
Sterile water: 60%～70%; Proline: 0.01%～0.05%;
Hydroxyamino: 0.01%～0.05%; Serine: 0.01%～0.05%;
Cystine: 0.05%～0.1%; Glucose: 2%～5%;
Linoleic acid: 2%～5%;
Wherein, selecting the gross weight of above-mentioned each raw material is 100%;
Its concrete making step comprises:
A), Yellow meal worm larva dry powder and barley worm larva dry powder blend is even;
B), the mixture of step a adds sterile water under the condition that stirs, this solution left standstill 12h for preparing is for subsequent use;
C), in the solution of step b, add proline, hydroxyamino, serine, cystine, linoleic acid and glucose, mix and to obtain the artificial feed finished product.
2. a kind of artificial extensive method of raising dastarcus helophoroides according to claim 1 is characterized in that, described artificial feed finished product carries out preservative treatment by in sorbic acid, Sodium Benzoate, the natamycin one or more.
3. a kind of artificial extensive method of raising dastarcus helophoroides according to claim 1, it is characterized in that, long 20 ~ the 25cm of described crisper, wide 8～10cm, high 5～8cm, spreading the absorbent cotton thickness that enters is 0.3～0.5cm, and the artificial feed amount for preparing of evenly pouring in each crisper is 60～80g.
4. a kind of artificial extensive method of raising dastarcus helophoroides according to claim 1 is characterized in that, the dastarcus helophoroides ovum quantity that is about to hatching on the dastarcus helophoroides ovum card of described hatching is 200～400, has one of ovum to face up, and is placed on the absorbent cotton.
5. a kind of artificial extensive method of raising dastarcus helophoroides according to claim 1 is characterized in that, the temperature setting of the climatic cabinate of described placement dastarcus helophoroides ovum card is set to 24～28 ℃, and humidity is set to 65%～80%, and illuminance is 0.
6. a kind of artificial extensive method of raising dastarcus helophoroides according to claim 1 is characterized in that, the described thickness that is layered on the absorbent cotton on the dastarcus helophoroides mature larva is 0.5～1cm, and needs all the dastarcus helophoroides mature larva to be covered.
7. a kind of artificial extensive method of raising dastarcus helophoroides according to claim 1 is characterized in that, described climatic cabinate temperature setting for placing mature larva is set to 24～28 ℃, and humidity is set to 75%～80%, and illuminance is 0.
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| CN105918713A (en) * | 2016-05-16 | 2016-09-07 | 遵义市林业科学研究所 | Dastarcus helophoroides feed and preparation method thereof |
| CN106616070B (en) * | 2016-09-19 | 2020-06-05 | 云南农业大学 | Artificial feed for Chinese chestnut weevil, breeding method and application of aged larva or pupa |
| CN110612946A (en) * | 2019-10-23 | 2019-12-27 | 广西壮族自治区林业科学研究院 | Method and device for breeding dastarcus helophoroides larvae |
| CN111771819B (en) * | 2020-07-09 | 2022-08-05 | 武汉市园林科学研究院 | Application of galleria mellonella larva as intermediate host in artificial breeding of dastarcus helophoroides |
| CN112400811A (en) * | 2020-11-25 | 2021-02-26 | 江苏省林业科学研究院 | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius |
| CN112913785B (en) * | 2021-01-29 | 2022-08-02 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN113180009B (en) * | 2021-04-01 | 2024-01-30 | 江苏省林业科学研究院 | Method for raising and preserving artificial habitat of adult Dastarcus helophoroides |
| CN115119805B (en) * | 2022-06-01 | 2023-05-12 | 中国林业科学研究院森林生态环境与自然保护研究所 | Efficient breeding method for dastarcus helophoroides |
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| JP4523856B2 (en) * | 2005-02-24 | 2010-08-11 | ヤシマ産業株式会社 | Control material and control method for piercing pests |
| CN101543265B (en) * | 2008-03-28 | 2012-11-14 | 李孟楼 | Dastarcus longulus sharp larva artificial feed |
| CN101574075A (en) * | 2008-05-05 | 2009-11-11 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial propagation technology of mallambyx raddei dastarcus helophoroides and technology for applying dastarcus helophoroides for controlling mallambyx raddei |
| CN101589696B (en) * | 2008-05-28 | 2013-06-05 | 中国林业科学研究院森林生态环境与保护研究所 | Technique for artificial propagation of apriona dastarcus helophoroides and preventive treatment of apriona swainsoni hope |
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